A composition for accelerating fat decomposition and its preparation method and application

Abstract

The present invention discloses a composition for accelerating fat decomposition, a preparation method and an application thereof, and belongs to the technical field of weight management. The composition comprises raw materials by weight: 5-15 parts of L-carnitine, 1-5 parts of citrus fiber, 0.5-5 parts of honeysuckle pollen, 0.1-3 parts of cassia seeds and 2-6 parts of white kidney bean extract. The preparation method comprises crushing cassia seeds and citrus fiber, mixing with L-carnitine, honeysuckle pollen and white kidney bean extract, and sieving. The present invention utilizes the compounding of L-carnitine, citrus fiber, honeysuckle pollen, cassia seeds and white kidney bean extract to play a synergistic effect in the decomposition of subcutaneous fat and visceral fat, can effectively reduce fat accumulation, and has a better fat reduction effect.

Description

Technical Field

[0001] The present invention relates to the technical field of weight control, and in particular to a composition for accelerating fat decomposition, and a preparation method and application thereof. Background Art

[0002] With the development of economy, people's living standards are constantly improving. Overnutrition and high-fat food intake have led to obesity in some people, and the incidence of obesity has increased year by year. The obesity rate has reached an alarming level in both developed and developing countries. The World Health Organization defines obesity as a disease, not just overweight. Obesity is a state caused by excessive accumulation of body fat, especially triglycerides, and is a multi-factorial chronic metabolic disease. Obesity not only brings troubles to people's appearance, but also poses a huge threat to human health. Studies have found that obesity is closely related to coronary heart disease, stroke, cerebral thrombosis, hypertension, hyperlipidemia, hyperglycemia and other diseases, which are harmful to human health.

[0003] CN116210904A discloses a fat-clearing and detoxifying composition, comprising the following raw materials: cassia seed extract, hemp seed extract, Cordyceps militaris extract, kelp extract, Sterculia lychnophora extract, licorice extract, houttuynia extract, Prunus mume extract, polygonatum extract, wolfberry extract, prune juice concentrate, blueberry juice concentrate, acerola fruit powder, lotus leaf extract, honeysuckle pollen, honey, Poria extract, ginseng extract, scutellaria extract, apple fruit powder, apricot fruit powder, red date extract, carrot extract, lemon powder, celery extract, blood orange extract, inulin and probiotics. The invention comprehensively plays the role of nourishing the body and qi, nourishing the spleen, promoting qi and blood circulation, and finally plays the role of transporting water and dampness, reducing body fat, detoxifying and beautifying, and celery and inulin contain a lot of dietary fiber, which can make the body feel full easily.

[0004] CN117547570A discloses a composition containing probiotics, which comprises, by weight, 5-10 parts of a fermentation composition, 1-3 parts of Lactobacillus acidophilus, 1-2 parts of Bifidobacterium lactis, 1-2 parts of Lactobacillus rhamnosus, 1-2 parts of Bacillus coagulans, 1-2 parts of stachyose, 1-2 parts of mannitol, 1-2 parts of isomaltooligosaccharide, 3-7 parts of noni fruit extract, 30-60 parts of distilled water, and 1-2 parts of tea oil. The fermentation composition comprises the following ingredients: The invention comprises 10-45 parts of L-carnitine, 8-40 parts of green coffee bean extract, 8-35 parts of tea polyphenols, 0.5-10 parts of Gynostemma pentaphyllum, 1-20 parts of lotus leaf, 3-30 parts of Poria cocos, 3-30 parts of Cassia seed, 5-20 parts of hawthorn, 3-20 parts of citron and 2-15 parts of chrysanthemum. The invention can significantly inhibit the weight gain induced by a high-fat diet and reduce the weight of subcutaneous fat and visceral fat.

[0005] Although there are many weight loss products on the market, their efficacy is far from satisfactory. Therefore, it is still very challenging to develop a weight loss product with clear efficacy and better effect. Summary of the invention

[0006] The purpose of the present invention is to provide a composition for accelerating fat decomposition, a preparation method and application thereof, wherein the composition has a synergistic effect in the decomposition of subcutaneous fat and visceral fat, can effectively reduce fat accumulation, and has a better fat reduction effect.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a composition for accelerating fat decomposition, which is composed of L-carnitine, citrus fiber, honeysuckle pollen, cassia seed and white kidney bean extract.

[0009] L-carnitine is an amino acid substance with multiple physiological functions in the body. It plays an important role in regulating the body's plasma carnitine content, muscle enzyme activity, and more importantly, lipid metabolism. Therefore, supplementing L-carnitine can promote acyl transport, thereby promoting fat metabolism, reducing the amount of fat in various tissues, and achieving the purpose of fat loss.

[0010] As a dietary fiber, citrus fiber has a very high water-holding capacity and can improve food quality and prevent intestinal diseases. Studies have shown that citrus fiber can increase gastrointestinal motility, further improve bowel movement speed and frequency; heptane in citrus fiber can inhibit amylase from forming neutral fat and reduce plasma cholesterol and triglyceride levels.

[0011] Honeysuckle has been praised as a good medicine for clearing heat and detoxifying since ancient times, and honeysuckle tea has a unique weight loss function.

[0012] Cassia seed is sweet, bitter, salty and slightly cold in nature. It has the effects of clearing the liver and improving eyesight, moistening the intestines and promoting bowel movements. Pharmacological studies have shown that Cassia seed has the effects of lowering blood lipids and blood pressure. Its active ingredient for lowering blood lipids is Cassia seed anthraquinone compounds.

[0013] White kidney bean extract contains highly active α-amylase inhibitors. At present, a white kidney bean essence product has been developed in China, which can inhibit the action of α-amylase, block starch decomposition, and reduce glucose absorption, thereby reducing postprandial blood sugar, reducing insulin secretion, and reducing fat synthesis.

[0014] The invention utilizes the combination of L-carnitine, citrus fiber, honeysuckle pollen, cassia seed and white kidney bean extract to play a synergistic role in the decomposition of subcutaneous fat and visceral fat, can effectively reduce fat accumulation, and has a better fat-reducing effect.

[0015] In some specific embodiments, the composition for accelerating fat decomposition comprises the following raw materials by weight: 5-15 parts of L-carnitine, 1-5 parts of citrus fiber, 0.5-5 parts of honeysuckle pollen, 0.1-3 parts of cassia seeds and 2-6 parts of white kidney bean extract.

[0016] In some specific embodiments, the composition for accelerating fat decomposition comprises the following raw materials by weight: 8-12 parts of L-carnitine, 2-4 parts of citrus fiber, 2-4 parts of honeysuckle pollen, 1-2 parts of cassia seed and 3-5 parts of white kidney bean extract.

[0017] In some specific embodiments, the composition for accelerating fat decomposition comprises the following raw materials by weight: 10 parts of L-carnitine, 3 parts of citrus fiber, 3 parts of honeysuckle pollen, 1.5 parts of cassia seeds and 4 parts of white kidney bean extract.

[0018] In a second aspect, the present invention provides a method for preparing the above composition for accelerating fat decomposition, comprising the following steps:

[0019] Crush cassia seed and citrus fiber, mix with L-carnitine, honeysuckle powder and white kidney bean extract, and sieve.

[0020] In a third aspect, the present invention provides use of a composition for accelerating fat decomposition in the preparation of a weight loss product.

[0021] In some specific embodiments, the weight loss product is a food or a medicine.

[0022] In some specific embodiments, the fat is subcutaneous fat and visceral fat.

[0023] In some specific embodiments, the fat is visceral fat.

[0024] In a fourth aspect, the present invention provides a weight loss product comprising the above composition for accelerating fat decomposition and auxiliary materials.

[0025] In some specific embodiments, the product of the present invention is in the form of granules, powder or blocks.

[0026] The beneficial effects of the present invention are:

[0027] The present invention utilizes the compound of L-carnitine, citrus fiber, honeysuckle pollen, cassia seed and white kidney bean extract to play a synergistic role in the decomposition of subcutaneous fat and visceral fat, can effectively reduce fat accumulation, and has a better fat-reducing effect. DETAILED DESCRIPTION

[0028] The following examples are only used to help understand the method of the present invention and its core idea. It should be noted that for those of ordinary skill in the art, without departing from the principles of the present invention, the present invention may also be subjected to several improvements and modifications, which also fall within the scope of protection of the claims of the present invention. The following description of the disclosed embodiments enables professionals and technicians in this field to implement or use the present invention. It will be apparent to professionals and technicians in this field that various modifications to these embodiments, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown herein, but may be applied to a wider range consistent with the principles and novel features disclosed herein. Unless otherwise defined, all technical and scientific terms used herein have the same meanings as those generally understood by those of ordinary skill in the art to which the present invention belongs.

[0029] The "composition for accelerating fat decomposition" and "product" described in the present invention are generally compositions comprising several compositions for accelerating fat decomposition (referred to as active ingredients) and acceptable excipients. In a specific embodiment, the active ingredients described in the present invention are provided in the composition in an effective amount (e.g., a therapeutically effective amount).

[0030] The "acceptable excipients" described in the present invention include inert diluents, dispersants and / or granulating agents, surfactants and / or emulsifiers, disintegrants, binders, preservatives, buffers, lubricants and / or oils. Excipients, colorants, coating agents, sweeteners and flavoring agents may also be present in the composition.

[0031] The composition of the present invention can be prepared by any method known in pharmacy. Generally speaking, these preparation methods include associating the active ingredient with a carrier or excipient and / or one or more other auxiliary ingredients, and then if necessary and / or desired, shaping and / or packaging the product into a desired single dose or multiple dose unit.

[0032] The composition of the present invention can be prepared according to known methods, such as the methods described in the general rules for preparation of the 16th edition of the Japanese Pharmacopoeia, the United States Pharmacopoeia, and the 9th edition of the European Pharmacopoeia. Depending on the dosage form, the composition of the present invention can be appropriately administered to a patient. For example, the composition of the present invention can be in the form of granules, powders, or blocks.

[0033] The active ingredient, excipients in the compositions herein will vary depending on the identity, size and / or condition of the subject being treated and further on the route of administration of the composition. The composition may contain between 0.1% and 100% (w / w) active ingredient.

[0034] As used herein, "treating" unless otherwise indicated, means reversing, alleviating, inhibiting the progression of, or preventing the condition or disorder, or one or more symptoms of such condition or disorder, to which the term applies.

[0035] The "composition for accelerating fat decomposition" and "product" of the present invention can also be used as raw materials in food and medicine. When the "composition for accelerating fat decomposition" and "product" of the present invention are used as raw materials, the preferred content ratio of the "composition for accelerating fat decomposition" and "product" is 10wt%-90wt%.

[0036] Hereinafter, examples of the present invention and comparative examples are shown to explain the composition for accelerating fat decomposition of the present invention in more detail, but the present invention is not limited thereto.

[0037] The present invention does not limit the sources of the raw materials used. Unless otherwise specified, the raw materials used in the present invention are all common commercial products in the technical field. Unless otherwise specified, the solvents used in the preparation of the test substance in the present invention are all deionized water.

[0038] The purchase information of raw materials is shown in Table 1.

[0039] Table 1 Raw material information

[0040]

[0041] Compositions for Accelerating Fat Decomposition in Examples 1-5 and Comparative Examples 1-4

[0042] The compositions for accelerating fat decomposition of different embodiments and comparative examples were prepared according to Table 2 and the following preparation methods.

[0043] Preparation method: Crush cassia seed and citrus fiber, pass through a 100-mesh sieve, mix with L-carnitine, honeysuckle pollen and white kidney bean extract, and pass through an 80-mesh sieve.

[0044] Table 2 Composition formula of the embodiment and comparative example (unit: weight part)

[0045]

[0046] Note: “-” in the table means not added.

[0047] Test Example 1: Inhibitory effect of the composition on fatty acid synthase

[0048] 1. Test materials

[0049] The compositions for accelerating fat decomposition prepared in Examples 1-5 and the compositions for accelerating fat decomposition prepared in Comparative Examples 1-4.

[0050] 2. Test methods

[0051] (1) Fresh duck liver → rinse → chop → homogenize → centrifuge at low temperature and low speed → centrifuge at low temperature and high speed → extract. Take fresh duck liver, cut off the fat and white tendons and fat on the liver with scissors; rinse with frozen physiological saline three times to remove blood and dirt on the surface; chop with scissors and add frozen phosphate buffer at a weight ratio of 1:1.8, and homogenize in a tissue homogenizer (stop for 20 seconds after homogenization for 10 seconds, otherwise it will easily heat up); put it into a centrifuge tube and centrifuge it at 8000r / min at 4℃ for 15 minutes to remove fat particles and cell nuclei; take the supernatant and centrifuge it at 12000r / min at 4℃ for 30 minutes. Take the supernatant and store it below -40℃ for later use.

[0052] (2) Take 100 μL of the test substance of different concentrations, add 200 μL of 1 mmoL / LEDTA, 0.9 mL of 0.1 mol / L potassium phosphate buffer at pH = 7 and substrate (400 μL of 6 μmoL / L acetyl CoA, 200 μL of 12 mol / L malonyl CoA, 150 μL of 37.5 μmoL / L NADPH), keep the temperature at 37°C in a water bath for 5 min, add 50 μL of supernatant to start the enzyme reaction, and investigate the activity characteristics of fatty acid synthase. The inhibitory effect of the test substance on fatty acid synthase can be calculated by comparing the absorbance value at 340 nm with that of the control group after 60 s of termination of the reaction. The control group is 1 mL of 0.1 mol / L potassium phosphate buffer.

[0053] According to the enzyme activity calculation formula, the fatty acid synthase inhibition rate formula under the same dilution conditions can be simplified as: inhibition rate (%) = [(A1-A2) / A1]*100%, where A1 and A2 are the absorbance values ​​of the control group and the test group, respectively.

[0054] 3. Test results

[0055] Table 3 Effect of different concentrations of the composition on the inhibition rate of fatty acid synthase

[0056]

[0057] As shown in Table 3, the composition prepared in Example 1 was prepared into test objects with different concentration gradients (0, 20, 40, 60, 80, 100 mg / mL), and the test objects were used to perform the fatty acid synthase inhibition test. The results showed that when the concentration of the composition was low, the fatty acid synthase inhibition rate was low, and as the concentration of the composition increased, the fatty acid synthase inhibition effect was better.

[0058] Table 4 Effect of the compositions of Examples and Comparative Examples on the inhibition rate of fatty acid synthase

[0059]

[0060] As shown in Table 4, the fatty acid synthase inhibition rate of the compositions prepared in Examples 1-5 is 70-85%, and the fatty acid synthase inhibition rate of the compositions prepared in Comparative Examples 1-4 is 48-66%, indicating that the compositions prepared in Examples 1-5 have significantly better inhibitory effects on fatty acid synthase than Comparative Examples 1-4, and the composition prepared in Example 1 has the best inhibitory effect on fatty acid synthase.

[0061] And by comparing the compositions obtained in Example 1 with those obtained in Comparative Examples 3 and 4, it can be seen that the combination of L-carnitine, citrus fiber, honeysuckle pollen, cassia seed and white kidney bean extract of the present invention has a synergistic effect in inhibiting fatty acid synthase.

[0062] Test Example 2: Method for quantitative detection of triglycerides

[0063] 1. Test materials

[0064] The compositions for accelerating fat decomposition prepared in Examples 1-5 and the compositions for accelerating fat decomposition prepared in Comparative Examples 1-4.

[0065] 2. Test methods

[0066] In order to quantitatively detect the effect of the composition on fat accumulation, the intracellular triglyceride content was determined using a triglyceride kit. 3T3-L1 adipocytes were incubated with the test substance in a cell culture incubator at 37°C, 5% CO2 for 24 hours. The supernatant was discarded and the cells were digested with 0.25% trypsin-EDTA. The accumulated triglyceride level in the cells was measured according to the instructions of the triglyceride assay kit (A110-1) to determine the degree of fat accumulation.

[0067] 3. Test results

[0068] Table 5 Effects of different concentrations of the composition on lipid accumulation inhibition rate

[0069]

[0070] As shown in Table 5, the composition prepared in Example 1 was prepared into test substances with different concentration gradients (0, 12.5, 25 μM), and the test substances were used to act on 3T3-L1 adipocytes. The results showed that the composition prepared in Example 1 inhibited lipid accumulation in a dose-dependent manner.

[0071] When the concentration of the composition prepared in Example 1 is 25 μM, compared with the concentration of the composition of 12.5 μM, the lipid accumulation of the composition prepared in Example 1 is reduced by 27.2%, which can significantly inhibit lipid accumulation.

[0072] Table 6 Effect of the compositions of Examples and Comparative Examples on the lipid accumulation inhibition rate

[0073]

[0074] As shown in Table 6, the lipid accumulation inhibition rate of the composition prepared in Examples 1-5 is 59-66%, and the lipid accumulation inhibition rate of the composition prepared in Comparative Examples 1-4 is 83-92%, indicating that the composition prepared in Examples 1-5 inhibits lipid accumulation better than Comparative Examples 1-4.

[0075] And by comparing the compositions obtained in Example 1 with those obtained in Comparative Examples 3 and 4, it can be seen that the combination of L-carnitine, citrus fiber, honeysuckle pollen, cassia seed and white kidney bean extract of the present invention has a synergistic effect in inhibiting lipid accumulation.

[0076] Test Example 3: Method for quantitative detection of glycerol

[0077] 1. Test materials

[0078] The compositions for accelerating fat decomposition prepared in Examples 1-5 and the compositions for accelerating fat decomposition prepared in Comparative Examples 1-4.

[0079] 2. Test methods

[0080] In order to quantitatively detect the effect of the composition on the cellular lipid decomposition, a glycerol kit was used for determination. 3T3-L1 adipocytes and the test substance were incubated in a cell culture incubator at 37°C and 5% CO2 for 24 hours. The culture medium used DMEM without serum and phenol red (serum and phenol red in the culture medium will affect the determination of the results). The supernatant was collected and centrifuged at 12000×g for 5 minutes. The glycerol content in the supernatant was measured according to the instructions of the glycerol enzyme assay kit (E1002) to determine the extent of lipid decomposition.

[0081] 3. Test results

[0082] Table 7 Effect of different concentrations of the composition on the glycerol release

[0083]

[0084] As shown in Table 7, the composition prepared in Example 1 was formulated into test substances with different concentration gradients (0, 12.5, 25 μM), and the test substances were used to act on 3T3-L1 adipocytes. The results showed that when the concentration of the composition was low, the glycerol content was low. As the concentration of the composition increased, the effect of lipid decomposition became better. The composition prepared in Example 1 was able to significantly increase the release of glycerol, and this result was consistent with the result of inhibiting lipid accumulation, indicating that the composition prepared in Example 1 showed a significant ability to promote lipid decomposition.

[0085] Table 8 Effect of the compositions of Examples and Comparative Examples on the release of glycerol

[0086]

[0087] As shown in Table 8, the glycerol release amount of the composition prepared in Examples 1-5 was 15.7-16.5 μM, and the glycerol release amount of the composition prepared in Comparative Examples 1-4 was 8.9-9.9 μM, indicating that the ability of the composition prepared in Examples 1-5 to promote lipid decomposition is better than that of Comparative Examples 1-4.

[0088] And by comparing the compositions obtained in Example 1 with those obtained in Comparative Examples 3 and 4, it can be seen that the combination of L-carnitine, citrus fiber, honeysuckle pollen, cassia seed and white kidney bean extract of the present invention plays a synergistic role in promoting lipid decomposition.

[0089] The above is a further description of the present invention in conjunction with specific embodiments, but these embodiments are merely exemplary and do not constitute any limitation on the scope of the present invention. It should be understood by those skilled in the art that the details and forms of the technical solution of the present invention may be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the scope of protection of the present invention.

Claims

1. A composition for accelerating fat decomposition, characterized in that: The raw materials are as follows: 5-15 parts of L-carnitine, 1-5 parts of citrus fiber, 0.5-5 parts of honeysuckle pollen, 0.1-3 parts of cassia seeds and 2-6 parts of white kidney bean extract by weight.

2. The composition for accelerating fat decomposition according to claim 1, characterized in that: The raw materials are as follows by weight: 8-12 parts of L-carnitine, 2-4 parts of citrus fiber, 2-4 parts of honeysuckle pollen, 1-2 parts of cassia seeds and 3-5 parts of white kidney bean extract.

3. The composition for accelerating fat decomposition according to claim 2, characterized in that: The raw materials are as follows by weight: 10 parts of L-carnitine, 3 parts of citrus fiber, 3 parts of honeysuckle pollen, 1.5 parts of cassia seeds and 4 parts of white kidney bean extract.

4. The method for preparing the composition for accelerating fat decomposition according to any one of claims 1 to 3, characterized in that: The method comprises the following steps: crushing cassia seed and citrus fiber, mixing with L-carnitine, honeysuckle powder and white kidney bean extract, and sieving.

5. Use of the composition for accelerating fat decomposition according to any one of claims 1 to 3 in the preparation of a weight loss product, wherein the product is a food.

6. The use according to claim 5, characterized in that: The fat includes subcutaneous fat and visceral fat.

7. The use according to claim 6, characterized in that: The fat is visceral fat.

8. A weight loss product, characterized in that: The product is a food, comprising the composition for accelerating fat decomposition according to any one of claims 1 to 3 and auxiliary materials.

9. The product according to claim 8, characterized in that The product is in the form of granules, powder or blocks.

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