Body acne-removing composition containing guaiacol extract and application of body acne-removing composition

Abstract

The invention discloses a body acne-removing composition containing guaiacol extract and application of the body acne-removing composition, and relates to the technical field of cosmetics. The invention provides an acne removing composition. The acne removing composition is prepared from the following components in parts by weight: 0.01 to 0.1 part of saponin A, 0.1 to 1 part of saponin B, 5 to 15 parts of buckwheat extract, 1 to 10 parts of cassia occidentalis seed extract and 1 to 10 parts of guaiacol extract. The saponin A comprises asperuloside and aucubin, the saponin B is ziyuglycoside, and the ziyuglycoside comprises ziyuglycoside I and ziyuglycoside II. When the saponin B and the saponin A cooperate with the buckwheat extract, the guaiacol extract and the cassia seed extract, on one hand, the whole acne-removing composition has excellent effects of removing heat, detoxifying and slowing down heat sensation, and can effectively prevent acne from growing on the skin; on the other hand, permeation of active substances in a skin barrier is promoted, dry skin is relieved, healing and repairing of existing acnes are accelerated, and the grease amount of the skin and the surface roughness of the skin are further improved.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, in particular to a body acne-removing composition containing a guaiac extract and application thereof. Background Art

[0002] Acne is one of the most common skin diseases worldwide, affecting 85% of the population. At the pathophysiological level, two factors, sebaceous glands and Propionibacterium acnes, play a crucial role. Acne commonly occurs on the face, chest, shoulders and upper back. Scars caused by acne (especially severe acne) can last a lifetime and have long-term psychosocial effects. Therefore, there are a large number of local and systemic treatments for acne, but compared with facial treatments, there is a lack of effective and gentle improvement options for the body, especially the back.

[0003] Regarding the improvement of back acne, there are the following problems: (1) The skin on the back is not as easy to clean as the face, and the stratum corneum is thicker and the metabolism is slower than that on the face. Excess oil in the body is blocked in the hair follicles and cannot be eliminated from the body, which makes it easier to form acne. (2) The back is the reflex area of ​​the lungs. If the lungs are hot or the meridians are blocked, it is easy for dampness to accumulate in the body and form toxins, causing acne on the back. A constitution prone to dryness and heat or endocrine disorders can easily cause acne. At present, most existing technologies use acidic substances to promote skin exfoliation, which is irritating and easily damages the skin barrier, causing dullness or acne pits after acne. Substances that work on the face are not ideal for improving the acne situation on the back skin, and are expensive and of poor practicality. In addition, most facial acne-removing ingredients do not have the effect of removing heat and detoxification, and the effect of acne removal on the back is not obvious.

[0004] Therefore, an acne-removing composition that has the effects of removing heat and detoxifying, reducing heat sensation, and can effectively prevent skin acne while accelerating the healing and repair of existing acne and improving acne pits and acne marks has broad application prospects. Summary of the invention

[0005] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a body acne-removing composition containing guaiac extract and its application, which has the effects of expelling heat and detoxifying, and alleviating heat sensation, can effectively prevent skin acne, and can accelerate the healing and repair of existing acne, and improve acne pits and acne marks.

[0006] To achieve the above object, the technical scheme adopted by the present invention is: an anti-acne composition, comprising the following components in parts by weight: 0.01-0.1 parts of saponin A, 0.1-1 parts of saponin B, 5-15 parts of buckwheat extract, 1-10 parts of Cassia seed extract, and 1-10 parts of guaiac extract; the saponin A comprises calendula and aucubin, the structural formula of calendula is shown in formula (I), and the structural formula of aucubin is shown in formula (II); the saponin B is sanguisorba saponins, the sanguisorba saponins comprise sanguisorba saponin I and sanguisorba saponin II, the structural formula of sanguisorba saponin I is shown in formula (III), and the structural formula of sanguisorba saponin II is shown in formula (IV);

[0007]

[0008] Sanguisorba officinalis, as a member of the Rosaceae family, has a variety of pharmacological properties, such as anti-oxidation. As the main active compound of Sanguisorba officinalis, Sanguisorba saponins have a variety of biological activities. Sanguisorba saponins can be divided into Sanguisorba saponin I and Sanguisorba saponin II. Sanguisorba saponin I has the ability to resist inflammation and promote cell survival in vitro. Sanguisorba saponin II is the metabolite of Sanguisorba saponin I and plays a role in inhibiting tumors. Eucommia ulmoides contains more than 100 active ingredients such as lignans, iridoids, phenylpropanoids, flavonoids and polysaccharides, which have multiple pharmacological effects such as lowering blood pressure, blood lipids and blood sugar, as well as antibacterial, anti-inflammatory, antioxidant and liver and kidney protection. Buckwheat extract is mainly derived from buckwheat seeds, leaves and flowers. The dietary fiber and flavonoids in buckwheat extract not only have antioxidant effects, but also help repair the skin barrier, especially in improving skin hydration and enhancing the skin's barrier function; Guaiac wood extract has the effects of antibacterial and anti-inflammatory, promoting tissue regeneration and repairing the skin barrier.

[0009] The inventors of the present application found in the actual experimental process that when saponin B (including sanguisorba officinalis saponin I and sanguisorba officinalis saponin II) and saponin A (including scutellaria baicalensis and aucubin) work together, the structural similarity gives them affinity for targets in the organism, and the mechanism of action of saponin B and saponin A can complement each other, enhancing the overall effect of the anti-acne composition in heat removal and detoxification, and mitigating heat sensation, thereby effectively preventing skin acne; and the sugar part of the saponin compound helps the substance to penetrate the skin barrier. The present invention can further improve the effect of heat removal and detoxification, and mitigating heat sensation without increasing side effects by rationally allocating the weight portions of saponin B and saponin A. The rational allocation of saponin B and saponin A helps the penetration of active substances in the skin barrier, and is suitable for back skin. The inventors of the present application found in the actual experimental process that the combination of buckwheat extract, guaiac extract, and cassia seed extract can further relieve skin allergies and redness, improve skin tolerance, and reduce allergic reactions. Therefore, when saponin B (including sanguisorbaside I and sanguisorbaside II) and saponin A (including calendulaside and aucubin) are used in synergy with buckwheat extract, guaiac extract, and cassia seed extract, on the one hand, the anti-acne composition as a whole has excellent effects in heat removal and detoxification, and reducing heat sensation, and can effectively prevent skin acne; on the other hand, it promotes the penetration of active substances in the skin barrier, relieves dry skin, accelerates the healing and repair of existing acne, and further improves the amount of skin oil and skin surface roughness. The above components work together to improve the acne microenvironment, acne pits and acne marks.

[0010] Preferably, the anti-acne composition comprises the following components in parts by weight: 0.05-0.08 parts of saponin A, 0.3-0.5 parts of saponin B, 8-12 parts of buckwheat extract, 6-8 parts of Cassia seed extract, and 5-8 parts of Guaiac wood extract.

[0011] Preferably, in the anti-acne composition, the mass percentage of saponin A is 0.25-0.28%.

[0012] Preferably, in the anti-acne composition, the mass percentage of saponin B is 1.3-1.8%.

[0013] Preferably, in the anti-acne composition, the total mass percentage of saponin A and saponin B is 1.6-2.1%.

[0014] The inventors of the present application found in the actual experimental process that the weight parts of saponin A, saponin B, buckwheat extract, cassia seed extract, and guaiac extract will affect the performance of the product. When the weight parts of the components are further selected to be within the above-mentioned range, the obtained product has a better effect of removing heat and detoxifying, reducing heat sensation and preventing skin acne, and further promotes the penetration of active substances in the skin barrier, relieves skin allergies and redness and swelling, and enhances the repair effect on existing acne.

[0015] Preferably, the weight ratio of aucubin to aucubin is 1:(1.8-4.2); and / or the weight ratio of sanguisorbaside I to sanguisorbaside II is (11-14):1.

[0016] Further preferably, the weight ratio of the aucubin to the aucubin is 1:(2-2.5); and / or the weight ratio of the sanguisorbaside I to the sanguisorbaside II is (12-13):1.

[0017] The inventors of the present application found in the actual experimental process that when the saponin A includes saponin and aucubin, and saponin and aucubin are in the above-mentioned specific weight ratio, the obtained product has better heat removal and detoxification effects, reduces heat sensation and prevents skin acne, and further promotes the penetration of active substances in the skin barrier, relieves skin allergies and redness and swelling, and enhances the repair effect on existing acne.

[0018] The inventors of the present application found in the actual experimental process that when the saponin B includes Sanguisorbaside I and Sanguisorbaside II, and Sanguisorbaside I and Sanguisorbaside II are in the above-mentioned specific weight ratio, the obtained product has better heat removal and detoxification effects, reduces heat sensation and prevents skin acne, and further promotes the penetration of active substances in the skin barrier, relieves dry skin, accelerates the healing and repair of existing acne, and further improves the amount of skin oil and skin surface roughness.

[0019] In addition, the present invention provides application of the anti-acne composition in preparing skin products.

[0020] Furthermore, the present invention provides a skin care product comprising the acne-removing composition. Optionally, the skin care product comprises at least one of a toner, a lotion, a cream, a mask, an essence, a body lotion, and a spray.

[0021] Optionally, the present invention provides a skin product comprising the following components in percentage by mass: 1-5% of an anti-acne composition, 5-40% of a cosmetic base, and the remainder being water; the cosmetic base comprising at least one of a moisturizer, a thickener, an emulsifier, a skin conditioner, an antioxidant, and a moisturizer.

[0022] Optionally, the present invention provides an anti-acne body lotion comprising the following components in percentage by weight: 1-5% of an anti-acne composition, 1-10% of a moisturizer, 0.1-0.5% of a thickener, 0.5-5% of an emulsifier, 0.05-1% of a skin conditioner, 0.5-1% of an antioxidant, 2-12% of an emollient, and the balance being deionized water.

[0023] Exemplarily, the moisturizer includes at least one of glycerin, butylene glycol, low molecular weight sodium hyaluronate, and 1,2-hexanediol; the thickener includes at least one of xanthan gum, carbomer 980, high molecular weight cellulose, and polyacrylate cross-linked polymer-6; the emulsifier includes at least one of C12-20 alkyl glucoside, C14-22 alcohol, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, polyglyceryl-6 distearate, and a mixture of arachidyl alcohol / behenyl alcohol / arachidyl glucoside; the skin conditioner includes at least one of trehalose, allantoin, and hyaluronic acid; the antioxidant includes at least one of tocopheryl acetate, p-hydroxyacetophenone, and ascorbic acid; the emollient includes at least one of polydimethylsiloxane, avocado tree (BUTYROSPERMUM PARKII) fruit fat, caprylic / capric triglyceride, petrolatum, and stearyl alcohol;

[0024] Compared with the prior art, the beneficial effects of the present invention are as follows: when saponin B (including sanguisorbaside I and sanguisorbaside II) and saponin A (including calendulaside and aucubin) are used in synergy with buckwheat extract, guaiac extract, and cassia seed extract, on the one hand, the anti-acne composition as a whole has excellent effects in heat removal and detoxification, and in reducing heat sensation, and can effectively prevent acne on the skin; on the other hand, it promotes the penetration of active substances in the skin barrier, relieves dry skin, accelerates the healing and repair of existing acne, and further improves the amount of skin oil and the roughness of the skin surface. The above components work together to improve the acne microenvironment, acne pits and acne marks. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 Figure 1 is a diagram of the swimming trajectory of zebrafish embryos in the thermal test; wherein, Figure (a) is a diagram of the swimming trajectory of zebrafish embryos in Example 6, and Figure (b) is a diagram of the swimming trajectory of zebrafish embryos in Comparative Example 1; Figure (c) is a diagram of the swimming trajectory of zebrafish embryos in the positive control group; and Figure (d) is a diagram of the swimming trajectory of zebrafish embryos in the model control group.

[0026] Figure 2 This is a comparison of the papules on the back skin before and after 28 days of use in Application Example 6;

[0027] Figure 3 This is a comparison of acne on the back skin before and after 28 days of use of Application Example 6. DETAILED DESCRIPTION

[0028] In order to better illustrate the purpose, technical scheme and advantages of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are the same batch of raw materials.

[0029] The raw materials used in the present invention are further described:

[0030] Synechoside: purchased from Bailingwei Technology Co., Ltd.;

[0031] Aucubin: purchased from Bailingwei Technology Co., Ltd.;

[0032] Sanguisorba officinalis saponin I: purchased from Shanghai Yien Chemical Technology Co., Ltd.;

[0033] Sanguisorba officinalis saponin II: purchased from Bailingwei Technology Co., Ltd.;

[0034] Buckwheat extract: product number SXHLWWZTQW, purchased from Shaanxi Hengling Natural Biological Products Co., Ltd.;

[0035] Cassia seed extract: product number L1-CY1139, purchased from Zhenghe (Guangzhou) Biotechnology Co., Ltd.;

[0036] Guaiac wood extract: Product No. L2-DZ-SCB3675-X, purchased from Zhenghe (Guangzhou) Biotechnology Co., Ltd.

[0037] Barley extract: Product No. FDGS-009, purchased from Xi'an Feida Biotechnology Co., Ltd.;

[0038] Redwood seed extract: product number HMZ, purchased from Xi'an Pris Bioengineering Co., Ltd.;

[0039] Milo wood extract: item number 000001, purchased from Shanxi Yirun Biotechnology Co., Ltd.

[0040] Commercially available Eucommia ulmoides: purchased from Shanghai Yuanye Biotechnology Co., Ltd. (The ratio of aucubin to aucubin is 1:5.5)

[0041] Commercially available Sanguisorba officinalis: purchased from Haozhou Jinlin Pharmaceutical Co., Ltd. (The ratio of Sanguisorba officinalis saponin I and Sanguisorba officinalis saponin II is 19:1)

[0042] The content ratio of aucubin and calophylloside in commercially available Eucommia ulmoides was detected by HPLC.

[0043] The method is as follows: add an appropriate amount of Eucommia ulmoides powder to a round-bottom flask, add 25 times 50% methanol, extract by ultrasonic for 40 minutes, filter with a 0.22μ microporous filter membrane, and save the filtrate. HPLC conditions are as follows: chromatographic column: Thermo Hypersil GoldC18 (250mm×4.6mm×5μm); mobile phase: methanol (A)-0.5% phosphoric acid solution (B), gradient elution (0~30min, A: 5%→10%, B: 95%→90%; 30~70min, A: 10%→25%, B: 90%→75%); detection wavelength is 206nm (0-15min), 236nm (15-55min), 360nm (55-120min); injection volume: 5μL; flow rate 1.0mL / min. The results show that the ratio of aucubin to aucubin is 1:5.5.

[0044] The content ratio of sanguisorbaside I and sanguisorbaside II in commercially available sanguisorba officinalis was detected by HPLC.

[0045] The method is as follows: take an appropriate amount of Sanguisorba officinalis powder, add 100 times 80% methanol, ultrasonically treat for 40 minutes, filter with a 0.22μ microporous filter membrane, and save the filtrate. HPLC conditions are: chromatographic column: Thermo Synoronis C18 column (4.6mm×250mm, 5μm); mobile phase: 0.1% phosphoric acid water as mobile phase A, acetonitrile as mobile phase B, gradient elution, 0-13min, 65% A; 13-22min, 65%→50% A; 22-40min, 50% A. Flow rate: 1.0mL / min; detection wavelength: 203nm; column temperature: 35℃; injection volume: 10μL. The results showed that the ratio of Sanguisorba officinalis saponin I and Sanguisorba officinalis saponin II was 19:1.

[0046] Examples and Comparative Examples

[0047] Examples 1-16

[0048] The present invention provides an anti-acne composition, the components and weight portions of the anti-acne composition are shown in Table 1, and the preparation method of the anti-acne composition comprises the following steps:

[0049] The saponin B is dissolved in 1.2 times the amount of glycerin and ultrasonically dissolved; the saponin A, buckwheat extract, Cassia seed extract, and Guaiac extract are dissolved in 2 times the amount of distilled water and ultrasonically dissolved; the above substances are ultrasonically mixed to obtain the acne-removing composition.

[0050] Embodiment 17

[0051] Provided is an anti-acne composition, comprising the following components in parts by weight: 3 parts of commercially available Eucommia ulmoides, 7.5 parts of commercially available Sanguisorba officinalis, 10 parts of buckwheat extract, 8 parts of Cassia tora seed extract, and 7 parts of Guaiacum officinale extract;

[0052] The preparation method is as follows: weigh eucommia bark, sanguisorba officinalis, buckwheat extract, cassia seed extract and guaiac extract according to weight parts, and mix them evenly to obtain the anti-acne composition.

[0053] Table 1

[0054]

[0055]

[0056] Comparative Examples 1-10

[0057] The comparative example of the present invention provides an acne-removing composition, the components and weight portions of the acne-removing composition are shown in Table 2, and the preparation method of the acne-removing composition comprises the following steps:

[0058] The saponin B is dissolved in 1.2 times the amount of glycerin and ultrasonically dissolved; the saponin A, buckwheat extract, Cassia seed extract, and Guaiac extract are dissolved in 2 times the amount of distilled water and ultrasonically dissolved; the above substances are ultrasonically mixed to obtain the acne-removing composition.

[0059] If there is no relevant component, just do not add it. If there is a replacement component, the preparation method is the same.

[0060] Comparative Example 1

[0061] The present invention provides an anti-acne composition. Compared with Example 6, only the selection of saponin A is different, and aucubin is not included. Specifically, the composition comprises the following components in parts by weight: 0.07 parts of calendula officinalis, 0.35 parts of saponin B (Sanguisorba officinalis saponin I:Sanguisorba officinalis saponin II=13:1), 10 parts of buckwheat extract, 8 parts of Cassia tora seed extract, and 7 parts of guaiac extract.

[0062] Comparative Example 2

[0063] The present invention provides an anti-acne composition. Compared with Example 6, only the selection of saponin A is different, and it does not contain calendulaside. Specifically, it comprises the following components in parts by weight: 0.07 parts of aucubin, 0.35 parts of saponin B (Sanguisorba officinalis saponin I:Sanguisorba officinalis saponin II=13:1), 10 parts of buckwheat extract, 8 parts of Cassia tora seed extract, and 7 parts of guaiac extract.

[0064] Comparative Example 3

[0065] The present invention provides an acne-removing composition, compared with Example 6, only the selection of sanguisorba officinalis saponin is different, and sanguisorba officinalis saponin II is not included. Specifically, the composition comprises the following components in parts by weight: 0.07 parts of saponin A (calendula officinalis:aucubin=1:2.1), 0.35 parts of sanguisorba officinalis saponin I, 10 parts of buckwheat extract, 8 parts of cassia seed extract, and 7 parts of guaiac extract.

[0066] Comparative Example 4

[0067] The present invention provides an acne-removing composition. Compared with Example 6, only the selection of sanguisorba officinalis saponin is different, and sanguisorba officinalis saponin I is not included. Specifically, the composition comprises the following components in parts by weight: 0.07 parts of saponin A (calendula officinalis saponin:aucubin=1:2.1), 0.35 parts of sanguisorba officinalis saponin II, 10 parts of buckwheat extract, 8 parts of cassia seed extract, and 7 parts of guaiac extract.

[0068] Table 2

[0069]

[0070]

[0071] Application Examples

[0072] The application examples of the present invention provide a body lotion, the components (mass percentage) of the body lotion are shown in Table 3; wherein the compositions in application examples 1-17 respectively use the anti-acne compositions prepared in embodiments 1-17; the anti-acne compositions in comparative application examples 1-10 respectively use the anti-acne compositions prepared in comparative examples 1-10; the blank application example does not contain only the anti-acne composition; for example, the anti-acne composition used in application example 1 is the anti-acne composition in embodiment 1, the anti-acne composition used in application example 5 is the anti-acne composition in embodiment 5, the anti-acne composition used in application example 15 is the anti-acne composition in embodiment 15, and the like; the anti-acne composition used in application example 18 is the anti-acne composition prepared in embodiment 6.

[0073] Table 3

[0074]

[0075]

[0076] The preparation method of the body lotion comprises the following steps:

[0077] (1) Component A and water are mixed and stirred, heated to 80° C., homogenized at a speed of 1200 rpm for 5 minutes, and kept warm for later use to obtain prefabricated component A;

[0078] (2) Mixing component B, heating to 80° C., homogenizing at 1200 rpm for 5 min, and keeping warm for later use to obtain prefabricated component B;

[0079] (3) mixing the C components and heating them to 60° C. to melt them to obtain a prefabricated C component;

[0080] (4) The preformed component A is heated to 80° C., and the preformed component B is added at a rotation speed of 300 rpm, and stirred and mixed. Then, the temperature is lowered to 60° C., and the preformed component C is added at a rotation speed of 300 rpm, and stirred and mixed. Then, the temperature is lowered to below 45° C., and the anti-acne composition is added and stirred for 5 minutes. The stirring can be stopped, and the material is discharged to obtain an emulsion.

[0081] Performance Test-1 Acne-related Pathogen Inhibition Test

[0082] This effect example explores the inhibitory effect of the acne-removing composition prepared by Examples 1-17 and Comparative Examples 1-10 on Propionibacterium acnes. The standard strain of Propionibacterium acnes freeze-dried strain ATCC 6919 was purchased from Guangdong Microbial Culture Collection Center. Using the 96-well plate crystal violet staining method, the acne-removing composition was added to a certain concentration of bacterial solution so that its final dosing mass concentration was 100 μg / mL, and cultured under anaerobic conditions at 37°C for 7 days, the upper floating cells were discarded, washed with sterile water, and formaldehyde was added to fix for 10 minutes, removed and air-dried for 5 minutes, and the biofilm was stained with 0.1% crystal violet for 20 minutes. After removing the dye, 95% ethanol was added for decolorization for 15 minutes, and 100 μL of the decolorizing solution was taken in a blank well plate. The absorbance was measured at a wavelength of 570 nm using an ELISA instrument. The biofilm of the control group without adding the composition was 100%, and the percentage of biofilm formation in each experimental group was calculated. The results are shown in Table 4.

[0083] Table 4

[0084]

[0085]

[0086] Note: Compared with the blank control group, the significance is indicated by *, P value < 0.05 is indicated by *, P value < 0.01 is indicated by **, P value < 0.001 is indicated by ***, and P value > 0.05 is indicated by ns.

[0087] Propionibacterium acnes, the main pathogenic bacteria of acne, plays a key role in pathogenicity. Inhibiting or killing Propionibacterium acnes is of great significance for the early prevention and treatment of acne. This experiment tests the inhibition rate of Propionibacterium acnes biofilm to evaluate the antibacterial ability of the acne-removing composition. Figure 2-3It can be seen that the biofilm formation rate of the acne-removing composition prepared in the embodiment of the present invention is below 70%, indicating that the acne-removing composition of the present invention has a significant inhibitory effect on Propionibacterium acnes. Figure 2 This is a comparison of the papules on the back skin before and after 28 days of use in Application Example 6; Figure 3 This is a comparison of acne on the back skin before and after 28 days of use of Application Example 6.

[0088] From the comparison of Examples 1-6, it can be seen that the weight parts of saponin A, saponin B, buckwheat extract, cassia seed extract, and guaiac extract will affect the performance of the product. When the weight parts of the components are further selected to be within the range of "saponin A 0.05-0.08 parts, saponin B 0.3-0.5 parts, buckwheat extract 8-12 parts, cassia seed extract 6-8 parts, guaiac extract 5-8 parts", the obtained product has a better inhibitory effect on Propionibacterium acnes.

[0089] By comparing Example 1 with Examples 7-11, it can be seen that when the saponin A includes aucubin and aucubin, and the weight ratio of aucubin and aucubin is "the weight ratio of aucubin to aucubin is 1: (2-2.5)", the obtained product has a better inhibitory effect on Propionibacterium acnes.

[0090] From the comparison between Example 1 and Examples 12-16, it can be seen that saponin B includes sanguisorbaside I and sanguisorbaside II. When sanguisorbaside I and sanguisorbaside II are in a specific weight ratio of "the weight ratio of sanguisorbaside I to sanguisorbaside II is (12-13):1", the obtained product has a better inhibitory effect on Propionibacterium acnes.

[0091] It can be seen from the comparison between the embodiments and the comparative examples that the saponin B and saponin A of the present invention, as well as the buckwheat extract, the Cassia seed extract and the Guaiac extract act synergistically and none of them can be missing.

[0092] Performance Test-2 Thermal Test

[0093] Acne can cause facial flushing or long-term redness, and internal dampness and heat are also one of the important factors that cause acne on the body. This effect example explores the improvement effect of the anti-acne composition prepared by Examples 1-17 and Comparative Examples 1-10 on thermal sensation. Zebrafish have a capsaicin pathway and can also be activated at high temperatures, reaching a peak at 37°C or above, which is manifested as a significant increase in swimming speed. The application software measures the movement distance of zebrafish embryos, compares the changes in the movement distance of zebrafish embryos in the test substance treatment group and the model control group, and calculates the movement distance shortening rate to evaluate the effect of the anti-acne composition in reducing thermal sensation. Using a reliable source (China National Zebrafish Resource Center) wild-type AB strain zebrafish (Danio rerio) spawning test, the breeding water temperature should be controlled at 26-28.5°C, and the indoor temperature is recommended to be controlled at 20-25°C. After the fish eggs were taken out from the egg collection box, the fish embryos were cleaned with fish embryo culture medium, healthy zebrafish embryos were selected, and cultured at 28° C. at a density of no more than 1 fish embryo in 200 μL fish embryo culture medium until 5 days after fertilization.

[0094] The anti-acne composition and dimethyl sulfoxide were mixed at a ratio of 1:1 (weight g: volume mL), ultrasonically treated for 10 minutes, added to fish embryo culture medium to make a concentration of 0.13%, oscillated for 30 seconds, and centrifuged at 6500 r / min for 10 minutes. The supernatant was taken for testing.

[0095] The test was divided into groups: a model control group (fish embryo culture medium), a positive control group (0.05 g / L 4-tert-butyl cyclohexanol solution prepared with fish embryo culture medium) and a test substance group (acne-removing composition).

[0096] Model control group: 12 fish embryos were randomly selected and transferred to a 96-well plate, with each well containing one fish embryo and 0.2 mL of fish embryo culture medium.

[0097] Positive control group: 12 fish embryos were randomly selected and transferred to a 96-well plate, with each well containing one fish embryo and 0.2 mL of 4-tert-butyl cyclohexanol solution;

[0098] Test group: 12 fish embryos were randomly selected and transferred to a 96-well plate, with each well containing one fish embryo and 0.2 mL of the acne-removing composition.

[0099] Each group was placed in a 28±1℃ incubator for 2h. Then, the cells were placed in a zebrafish motion recorder (DanioVision, Noldus Information Technology) preheated to 38℃, and the infrared tracking software EthoVision XT was used to record the swimming trajectory of each zebrafish embryo within 5min in a dark environment. The 0.18mL solution in each well was replaced with fish embryo culture medium, and then placed in a 28℃ incubator for 24h to calculate the survival rate of the fish embryos. The pixel intensity of each well was measured as the movement distance of each fish embryo.

[0100] Calculate the shortening rate of movement distance: shortening rate = (CT) / C × 100%; where: T is the average movement distance of fish embryos in the test substance treatment group; C is the average movement distance of fish embryos in the model control group; perform a two-tailed T test on the average movement distance of fish embryos in the test substance treatment group and the average movement distance of the model control group to obtain the P value.

[0101] The results are shown in Table 5.

[0102] Table 5

[0103]

[0104]

[0105] Note: Compared with the model control group, significance is indicated by *, P value < 0.05 is indicated by *, P value < 0.01 is indicated by **, P value < 0.001 is indicated by ***, and P value > 0.05 is indicated by ns.

[0106] Figure 1 Figure 1 is a diagram of the swimming trajectory of zebrafish embryos in the thermal test; wherein, Figure (a) is a diagram of the swimming trajectory of zebrafish embryos in Example 6, and Figure (b) is a diagram of the swimming trajectory of zebrafish embryos in Comparative Example 1; Figure (c) is a diagram of the swimming trajectory of zebrafish embryos in the positive control group; and Figure (d) is a diagram of the swimming trajectory of zebrafish embryos in the model control group.

[0107] From the experimental results in Table 5, it can be seen that the movement distance reduction rate of the acne-removing composition prepared in the embodiment of the present invention is above 65%, indicating that the acne-removing composition of the present invention has a better effect on improving thermal sensation.

[0108] From the comparison of Examples 1-6, it can be seen that the weight parts of saponin A, saponin B, buckwheat extract, cassia seed extract, and guaiac extract will affect the performance of the product. When the weight parts of the components are further selected within the range of "saponin A 0.05-0.08 parts, saponin B 0.3-0.5 parts, buckwheat extract 8-12 parts, cassia seed extract 6-8 parts, guaiac extract 5-8 parts", the obtained product has a better effect on improving the thermal sensation.

[0109] By comparing Example 1 with Examples 7-11, it can be seen that when the saponin A includes aucubin and aucubin, and the weight ratio of aucubin and aucubin is "1:(2-2.5)", the obtained product has a better effect on improving the thermal sensation.

[0110] By comparing Example 1 with Examples 12-16, it can be seen that saponin B includes sanguisorbaside I and sanguisorbaside II. When sanguisorbaside I and sanguisorbaside II are in a specific weight ratio of "the weight ratio of sanguisorbaside I to sanguisorbaside II is (12-13):1", the obtained product has a better effect on improving the thermal sensation.

[0111] It can be seen from the comparison of the embodiments and comparative examples that saponin B, saponin A, buckwheat extract, Cassia seed extract and Guaiac extract of the present invention act synergistically and none of them can be missing.

[0112] Performance test-3 Detection of back skin acne count, oiliness, and smoothness

[0113] The effect examples of the present invention verify the efficacy of application examples 1-18, comparative application examples 1-10 and blank application examples when applied to human bodies, specifically including the following methods: according to the "Technical Specifications for Safety of Cosmetics" (2015), subjects aged 18-60 were selected. Under normal circumstances, the subjects were subjected to a random test, with 8 people in each group, for a total of 29 groups, and continued to use for 28 days. Instrumental detection and clinical evaluation of skin indicators were performed on the 0th, 7th, 14th and 28th days of the test.

[0114] The specific steps of instrument measurement are as follows: After bathing every night, the subject takes 1.5mL of body lotion and applies it to the acne area on the back, and massages until absorbed. On the day of the subject's visit, the subject cleans with clean water without applying any products, and sits quietly in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 20 minutes. Instruments are used to quantify and photograph the skin condition, including VC20Plus to measure the roughness, Sebumeter SM 815 to test the amount of oil, and acne counts (papules, comedones) evaluated by doctors. Record the data and calculate the index improvement rate on the 28th day, index improvement rate = (value before use - value after use) / value before use × 100%.

[0115] The results are shown in Table 6.

[0116] Table 6

[0117]

[0118]

[0119] Note: Compared with the blank control group, the significance is indicated by *, P value < 0.05 is indicated by *, and P value > 0.05 is indicated by ns.

[0120] From the experimental results in Table 6, it can be seen that the anti-acne composition prepared in the embodiment of the present invention has a significant improvement effect on papules, acne, oil and skin roughness of the back skin, indicating that the body lotion prepared in the present invention plays an obvious role in improving acne, preventing acne and smoothing the skin. The above components work together to improve the acne microenvironment, improve acne pits and acne marks.

[0121] From the comparison of Examples 1-6, it can be seen that the weight parts of saponin A, saponin B, buckwheat extract, cassia seed extract, and guaiac extract will affect the performance of the product. When the weight parts of the components are further selected within the range of "saponin A 0.05-0.08 parts, saponin B 0.3-0.5 parts, buckwheat extract 8-12 parts, cassia seed extract 6-8 parts, guaiac extract 5-8 parts", the obtained body lotion has a better effect on improving papules, acne, oil and skin roughness on the back skin.

[0122] From the comparison between Example 1 and Examples 7-11, it can be seen that when the saponin A includes aucubin and aucubin, and the weight ratio of aucubin and aucubin is "the weight ratio of aucubin to aucubin is 1: (2-2.5)", the obtained body lotion has a better effect on improving papules, acne, oil and skin roughness of the back skin.

[0123] By comparing Example 1 and Examples 12-16, it can be seen that saponin B includes sanguisorbaside I and sanguisorbaside II. When sanguisorbaside I and sanguisorbaside II are in a specific weight ratio of "the weight ratio of sanguisorbaside I to sanguisorbaside II is (12-13):1", the resulting body lotion has a better effect on improving papules, acne, oil and skin roughness of the back skin.

[0124] It can be seen from the comparison of the embodiments and comparative examples that saponin B, saponin A, buckwheat extract, Cassia seed extract and Guaiac extract of the present invention act synergistically and none of them can be missing.

[0125] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. An anti-acne composition, characterized in that: The invention comprises the following components in parts by weight: 0.01-0.1 part of saponin A, 0.1-1 part of saponin B, 5-15 parts of buckwheat extract, 1-10 parts of cassia seed extract and 1-10 parts of guaiac extract; the saponin A comprises calendulaside and aucubin, the saponin B is sanguisorba officinalis saponin, and the sanguisorba officinalis saponin comprises sanguisorba officinalis saponin I and sanguisorba officinalis saponin II.

2. The anti-acne composition according to claim 1, characterized in that The invention comprises the following components in parts by weight: 0.05-0.08 parts of saponin A, 0.3-0.5 parts of saponin B, 8-12 parts of buckwheat extract, 6-8 parts of cassia seed extract and 5-8 parts of guaiac extract.

3. The anti-acne composition according to claim 1, characterized in that: In the anti-acne composition, the mass percentage of saponin A is 0.25-0.28%.

4. The anti-acne composition according to claim 1, characterized in that: In the anti-acne composition, the mass percentage of saponin B is 1.3-1.8%.

5. The anti-acne composition according to claim 1, characterized in that: In the anti-acne composition, the total mass percentage content of saponin A and saponin B is 1.6-2.1%.

6. The anti-acne composition according to claim 1, characterized in that: The weight ratio of the aucubin to the aucubin is 1:(1.8-4.2); and / or the weight ratio of the sanguisorbaside I to the sanguisorbaside II is (11-14):

1.

7. The anti-acne composition according to claim 6, characterized in that: The weight ratio of the aucubin to the aucubin is 1:(2-2.5); and / or the weight ratio of the sanguisorbaside I to the sanguisorbaside II is (12-13):

1.

8. Use of the anti-acne composition according to any one of claims 1 to 7 in the preparation of skin products.

9. A skin care product, characterized in that: The skin product comprises the anti-acne composition according to any one of claims 1 to 7.

10. A skin care product, characterized in that: The skin care product comprises the following components in percentage by weight: 1-5% of the anti-acne composition according to any one of claims 1 to 7, 5-40% of a cosmetic base, and the balance water; the cosmetic base comprises at least one of a moisturizer, a thickener, an emulsifier, a skin conditioner, an antioxidant, and a moisturizer.

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