A protein separation and detection device

Abstract

The present invention belongs to the technical field of protein separation detection, and discloses a protein separation detection device, including a detector, a chromatography column is vertically arranged above the detector, the bottom liquid outlet of the chromatography column is connected to the liquid inlet port of the detector through a hose, and a material taking mechanism for taking out cotton balls and sand particles is arranged in the inner cavity of the chromatography column. The present invention realizes the rapid removal of agglomerated sand particles and expanded cotton balls through the coordinated design of the chromatography column and the material taking mechanism, shortens the material taking time, improves the efficiency, and avoids the problem of damage to the inner cavity of the chromatography column during removal, improves the service life of the chromatography column, avoids the problem that the traditional chromatography column is not convenient for the rapid removal of agglomerated sand particles and expanded cotton balls, reduces the efficiency of protein separation detection, increases friction, and easily causes damage to the inner wall of the chromatography column.

Description

Technical Field

[0001] The invention belongs to the technical field of protein separation and detection, in particular to a protein separation and detection device. Background Art

[0002] Protein is an important component of all cells and tissues in the human body. When protein is converted into fat in the body, the acidity of the blood increases. All important components of the body require the participation of protein. When studying protein in the fields of life sciences and medicine, it is usually necessary to use a protein separation and detection device to purify and detect the protein.

[0003] However, in the prior art, proteins are usually separated and purified by precipitation, centrifugation, electrophoresis, ultrafiltration, chromatography, etc., and the separated and purified proteins are detected by a detector.

[0004] When using chromatography to separate and purify proteins in the prior art, the chromatography column is first fixed on a rack vertically to the ground, a cotton ball is stuffed into the lower part of the inner cavity of the chromatography column, and the cotton ball is covered with sand with a thickness of 2-5 mm. Subsequently, an eluent and a protein mixture to be separated and tested are added to the chromatography column, and the mixture flows downward under the action of gravity to achieve chromatographic separation and purification, and flows from the outlet at the lower end of the chromatography column to a detector for detection.

[0005] However, there are still corresponding disadvantages in actual use: after the sand covers the cotton balls, the sand particles are easily embedded in the gaps of the cotton balls. After the sand and cotton balls in the chromatography column are soaked in liquid, the adsorption force is enhanced and it is easy to form lumps. Moreover, the cotton balls are prone to swell and the squeezing pressure on the inner wall of the chromatography column is increased after being soaked in liquid. When multiple groups of proteins are separated and tested in turn, the sand and cotton balls need to be taken out and refilled after each group of protein separation and testing is completed. The traditional chromatography column is not convenient for quickly removing the agglomerated sand and swollen cotton balls, which reduces the efficiency of protein separation and testing. In addition, the sand particles embedded in the cotton balls and the agglomerated sand blocks increase the friction with the inner wall when they are taken out of the chromatography column, which is easy to damage the inner wall of the chromatography column and reduces the service life of the chromatography column. Therefore, it needs to be improved. Summary of the invention

[0006] Technical issues solved

[0007] In order to solve the problems raised in the above-mentioned background technology, the present invention provides a protein separation and detection device, which has the advantages of convenient operation, improved protein separation and detection efficiency, and the ability to protect the chromatography column. Through the coordinated design of the chromatography column, the material taking mechanism and other structures, it is convenient to quickly remove the agglomerated sand and swollen cotton balls, shortening the material taking time and improving the efficiency of protein separation and detection. At the same time, it avoids the problem of increased friction between the sand blocks and the swollen cotton balls and the inner wall when they are taken out of the chromatography column, thereby damaging the inner cavity of the chromatography column when they are taken out, thereby improving the service life of the chromatography column.

[0008] Technical Solution

[0009] To achieve the above object, the present invention provides the following technical solution: a protein separation detection device, comprising a detector, a chromatography column is vertically arranged above the detector, the bottom liquid outlet of the chromatography column is connected to the liquid inlet port of the detector through a hose, and a material taking mechanism for taking out cotton balls and sand particles is arranged in the inner cavity of the chromatography column;

[0010] The material taking mechanism includes a supporting plate placed at the lower part of the inner cavity of the chromatography column for supporting the cotton ball, an annular mounting shell is movably installed in the inner cavity of the supporting plate through a bearing, a filter shell that can move up and down is arranged in the inner cavity of the mounting shell, an annular block is fixedly installed on the outer ring of the mounting shell through a bracket, a group of cotton rolling rods that can extend into the inner cavity of the mounting shell for winding the cotton ball are equidistantly arranged in the circumference of the inner cavity of the annular block, a cleaning mechanism for cleaning the inner cavity of the chromatography column is arranged at one end of the cotton rolling rod away from the mounting shell, a transmission mechanism is arranged below the cotton rolling rod, a collection shell is threadedly installed on the lower part of the outer surface of the mounting shell, the bottom surface of the collection shell is movably connected to the bottom surface of the inner cavity of the chromatography column, and a driving mechanism for driving the cotton rolling rod to rotate is arranged on the upper surface of the supporting plate;

[0011] Among them, the bottom surface of the filter shell is movably connected to the bottom surface of the inner cavity of the chromatography column, and a group of spring compression rods for driving the filter shell to move downward are circumferentially equidistantly arranged in the inner cavity of the mounting shell. When the filter shell moves downward to the lower part of the inner cavity of the mounting shell, the cotton rolling rod can be driven by the transmission mechanism to extend into the upper part of the inner cavity of the mounting shell, and a group of discharge channels are circumferentially equidistantly opened in the middle part of the inner cavity of the mounting shell.

[0012] In the above technical scheme, preferably, the driving mechanism includes a group of connecting shells vertically fixedly mounted on the upper surface of the supporting plate, the outer side wall of the connecting shell is movably connected to the inner cavity of the chromatography column, the top surface of the connecting shell is fixedly mounted with an annular connecting block, the bottom surface of the connecting block is located in the inner cavity of the connecting shell and is rotatably mounted with a driving rod for driving the cotton rolling stick to rotate, a group of annular assembly blocks are arranged on the outer side of the connecting shell, the bottom surface of the assembly block is movably connected to the upper surface of the chromatography column, a group of assembly blocks extending into the inner cavity of the connecting shell are equidistantly fixedly mounted on the inner ring bearing of the assembly shell, the assembly blocks are slidably connected to the inner cavity of the connecting shell, a guide rod is fixedly mounted on the outer surface of the assembly block, a spiral groove is circumferentially opened on the outer surface of the driving rod, and the guide rod is movably connected to the inner cavity of the spiral groove.

[0013] In the above technical solution, preferably, the driving mechanism also includes a driving gear fixedly sleeved on the bottom of the driving rod, a gear ring fixedly sleeved on the upper part of the outer surface of the annular block and meshing with the driving gear, and a roller movably installed on the inner ring of the annular block through a bearing; the inner ring of the roller is key-connected to the outer surface of the cotton rolling swab, and the outer ring of the roller is movably connected to the bottom surface of the support plate.

[0014] In the above technical solution, preferably, the driving mechanism also includes a group of mounting grooves equidistantly arranged in the circumferential direction on the bottom surface of the assembly block, and a splint movably connected to the outer surface of the chromatography column and a screw threadedly installed on the outer ring of the assembly block are laterally slidably installed in the inner cavity of the mounting groove; one end of the screw passes through the inner cavity of the connecting shell, and one end of the screw is movably connected to the outer surface of the driving rod, and the splint is movably connected to the screw via a bearing.

[0015] In the above technical scheme, preferably, the transmission mechanism includes an installation channel equidistantly arranged at the lower part of the installation shell, a pin rod hinged in the inner cavity of the installation channel, vertical grooves equidistantly arranged on the outer surface of the filter shell, and a pin shell movably sleeved on one side of the outer surface of the cotton rolling rod through a bearing; one end of the pin rod is movably connected to the top surface of the inner cavity of the vertical groove, the other end of the pin rod is movably connected to the inner cavity of the pin shell, and a first torsion spring for driving the pin rod to rotate is arranged between the pin rod and the inner cavity of the installation channel.

[0016] In the above technical solution, preferably, a group of grooves are equidistantly opened circumferentially on one side of the cotton rolling rod close to the mounting shell, a scraper rod is hinged in the inner cavity of the groove, and a second torsion spring for driving the scraper rod to rotate is arranged between the scraper rod and the inner cavity of the groove.

[0017] In the above technical scheme, preferably, the cleaning mechanism includes a mounting rod slidably mounted on the outer end of the cotton rolling swab, a group of the outer surfaces of the mounting rods are movably mounted with an annular liquid storage shell through a bearing, a group of cleaning plates for scraping and cleaning residues attached to the inner cavity of the chromatography column are fixedly mounted equidistantly on the outer ring of the liquid storage shell, and a group of pressure nozzles for spraying cleaning agent on the inner cavity of the chromatography column are arranged on the outer surface of the cleaning plate; the inner cavity of the pressure nozzle is connected to the inner cavity of the liquid storage shell, and a spiral sheet is fixedly mounted on the outer surface of the mounting rod, and when the mounting rod rotates, the spiral sheet is driven to rotate to spray the cleaning agent in the liquid storage shell through the pressure nozzle.

[0018] In the above technical solution, preferably, a ""-shaped bracket is vertically fixedly installed on the side wall of the detector, and the chromatography column is detachably connected to the upper right side of the bracket.

[0019] Beneficial Effects

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] The filter element of the present invention is a filter element which is convenient for filtering out particles and is easily removed from the filter element by the filter element when it is removed from the filter element.

[0022] 2. The present invention adopts the coordinated design of structures such as the cotton rolling swab and the cleaning mechanism. When the cotton rolling swab moves and rotates circumferentially, it can drive the mounting rod to move and rotate circumferentially. The mounting rod can drive the liquid storage shell to drive the cleaning plate to move circumferentially to scrape and clean the inner cavity of the chromatography column. At the same time, when the mounting rod rotates, under the action of the spiral blade, the cleaning agent in the liquid storage shell can be sprayed out through the pressure nozzle to the inner wall of the chromatography column for cleaning, so that the residue attached to the inner cavity of the chromatography column can be cleaned while taking the material, which is convenient and fast, further improving the efficiency of protein separation and detection, avoiding the need for the operator to dismantle and clean the traditional chromatography column after each protein separation and detection before performing the next set of protein separation and detection, reducing the protein separation and detection effect and the problem of high labor intensity for the operator. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic diagram of the structure of the present invention;

[0024] Figure 2 It is a schematic diagram of the front cross-sectional structure of the chromatography column of the present invention;

[0025] Figure 3 It is a structural schematic diagram of the material taking mechanism of the present invention;

[0026] Figure 4 It is a partial front cross-sectional structural schematic diagram of the chromatography column and the material taking mechanism of the present invention;

[0027] Figure 5 for Figure 4 An enlarged schematic diagram of part A is shown;

[0028] Figure 6 for Figure 4 An enlarged schematic diagram of portion B is shown;

[0029] Figure 7 It is a partial structural exploded schematic diagram of the supporting plate, the mounting shell and the filter shell of the present invention;

[0030] Figure 8 This is a partial structural exploded schematic diagram of the mounting housing, gear ring, and filter housing of the present invention;

[0031] Fig. 9 It is a partial cross-sectional structural schematic diagram of the installation shell, the filter shell, the annular block, the cotton winding stick, the roller, and the transmission mechanism of the present invention;

[0032] Fig.10 It is a structural schematic diagram of the driving mechanism of the present invention;

[0033] Fig.11 It is a partial cross-sectional structural schematic diagram of the driving structure of the present invention.

[0034] In the figure: 1. detector; 2. chromatography column; 3. material taking mechanism; 31. support plate; 32. mounting shell; 33. filter shell; 34. annular block; 35. cotton roll; 36. collection shell; 37. spring compression rod; 38. discharge channel; 4. cleaning mechanism; 41. mounting rod; 42. liquid storage shell; 43. cleaning plate; 44. pressure nozzle; 5. transmission mechanism; 51. pin rod; 52. vertical groove; 53. pin shell; 6. driving mechanism; 61. connecting shell; 62. connecting block; 63. driving rod; 64. assembly block; 65. guide rod; 66. spiral groove; 67. driving gear; 68. gear ring; 69. roller; 610. clamping plate; 611. screw; 7. scraper rod. DETAILED DESCRIPTION

[0035] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0036] like Figures 1 to 11 As shown, the present invention provides a protein separation detection device, including a detector 1, the detector 1 is a prior art, and its structure and principle are not repeated here, a chromatography column 2 is vertically arranged above the detector 1, the bottom liquid outlet of the chromatography column 2 is connected to the liquid inlet port of the detector 1 through a hose, and a material taking mechanism 3 for taking out cotton balls and sand particles is arranged in the inner cavity of the chromatography column 2;

[0037] A ""-shaped bracket is vertically fixedly installed on the side wall of the detector 1, and the chromatography column 2 is detachably connected to the upper right side of the bracket;

[0038] The material taking mechanism 3 includes a supporting plate 31 placed at the lower part of the inner cavity of the chromatography column 2 for supporting the cotton ball, an annular mounting shell 32 is movably mounted in the inner cavity of the supporting plate 31 through a bearing, a filter shell 33 which can move up and down is arranged in the inner cavity of the mounting shell 32, an annular block 34 is fixedly mounted on the outer ring of the mounting shell 32 through a bracket, a group of cotton rolling rods 35 which can extend into the inner cavity of the mounting shell 32 for winding the cotton ball are equidistantly arranged in the inner cavity of the annular block 34 in the circumferential direction, a cleaning mechanism 4 for cleaning the inner cavity of the chromatography column 2 is arranged at one end of the cotton rolling rod 35 away from the mounting shell 32, a transmission mechanism 5 is arranged below the cotton rolling rod 35, a material collecting shell 36 is threadedly mounted on the lower part of the outer surface of the mounting shell 32, the bottom surface of the material collecting shell 36 is movably connected to the bottom surface of the inner cavity of the chromatography column 2, and a driving mechanism 6 for driving the cotton rolling rod 35 to rotate is arranged on the upper surface of the supporting plate 31;

[0039] Among them, the bottom surface of the filter shell 33 is movably connected to the bottom surface of the inner cavity of the chromatography column 2, and a group of spring compression rods 37 for driving the filter shell 33 to move downward are equidistantly arranged in the inner cavity of the mounting shell 32. When the filter shell 33 moves downward to the lower part of the inner cavity of the mounting shell 32, the cotton rolling rod 35 can be driven by the transmission mechanism 5 to extend into the upper part of the inner cavity of the mounting shell 32, and a group of discharge channels 38 are equidistantly opened in the middle part of the inner cavity of the mounting shell 32.

[0040] When separating and detecting the protein, first insert the material taking mechanism 3 into the chromatography column 2 so that the bottom surface of the collection shell 36 fits with the bottom surface of the inner cavity of the chromatography column 2, and at the same time, the bottom surface of the filter shell 33 fits with the bottom surface of the inner cavity of the chromatography column 2, so that the upper surface of the filter shell 33 and the upper surface of the supporting plate 31 are at the same horizontal position, and the material taking mechanism 3 inserted into the chromatography column 2 can be limited and fixed by the driving mechanism 6, and then the filler is placed on the supporting plate 31 in the chromatography column 2, and then the eluent and the protein mixture to be separated and detected are added to the chromatography column 2, and the mixture flows downward under the action of gravity to realize chromatographic separation and purification, and flows from the outlet at the lower end of the chromatography column 2 to the detector 1 for detection.

[0041] When taking out the material, the operator takes out the material taking mechanism 3 from the inner cavity of the chromatography column 2. During the taking out process, the filter housing 33 is driven downward by the elastic force of the spring compression rod 37, so that the upper part of the filter housing 33 moves to the lower part of the inner cavity of the mounting housing 32. When the filter housing 33 moves, the transmission mechanism 5 drives the cotton rolling stick 35 to extend into the upper part of the inner cavity of the mounting housing 32. At the same time, under the action of the driving mechanism 6, the cotton rolling stick 35 rotates while moving in the circumferential direction. When the cotton rolling stick 35 is in operation, The cotton balls on the supporting plate 31 can be rolled up and collected, and the sand blocks above the cotton balls can be broken up, so that the sand particles fall into the collecting shell 36 through the discharge channel 38 for collection, so that the agglomerated sand particles and the expanded cotton balls can be quickly removed, thereby shortening the material removal time and improving the efficiency of protein separation and detection. At the same time, the problem of increased friction between the sand blocks and the expanded cotton balls and the inner wall when they are taken out of the chromatography column 2, which may damage the inner cavity of the chromatography column 2 when they are taken out, is avoided, thereby improving the service life of the chromatography column 2.

[0042] It should be noted that when the cotton swab 35 moves and rotates circumferentially, it can drive the cleaning mechanism 4 to operate. The cleaning mechanism 4 can clean the residues attached to the inner cavity of the chromatography column 2 while taking the material, thereby further improving the efficiency of protein separation and detection, and avoiding the problem that the operator needs to dismantle and clean the traditional chromatography column 2 after each protein separation and detection before performing the next set of protein separation and detection, which reduces the protein separation and detection effect and increases the labor intensity of the operator.

[0043] like Fig.10 and Fig.11As shown, the driving mechanism 6 includes a group of connecting shells 61 vertically fixedly mounted on the upper surface of the supporting plate 31, the outer wall of the connecting shell 61 is movably connected to the inner cavity of the chromatography column 2, an annular connecting block 62 is fixedly mounted on the top surface of the connecting shell 61, the bottom surface of the connecting block 62 is located in the inner cavity of the connecting shell 61 and is rotatably mounted with a driving rod 63 for driving the cotton rolling stick 35 to rotate, an annular assembly block 64 is arranged on the outer side of a group of connecting shells 61, the bottom surface of the assembly block 64 is movably connected to the upper surface of the chromatography column 2, a group of assembly blocks 64 extending into the inner cavity of the connecting shell 61 are equidistantly fixedly mounted on the inner ring bearing of the assembly block 64, the assembly block is slidably connected to the inner cavity of the connecting shell 61, a guide rod 65 is fixedly mounted on the outer surface of the assembly block 64, a spiral groove 66 is circumferentially opened on the outer surface of the driving rod 63, and the guide rod 65 is movably connected to the inner cavity of the spiral groove 66.

[0044] When in use, the operator presses the assembly block 64 to fit it with the upper surface of the chromatography column 2, and lifts the connecting block 62 upward. During the lifting process, the upper part of the filter shell 33 is moved to the lower part of the inner cavity of the mounting shell 32 under the drive of the spring compression rod 37. At the same time, the cotton rolling stick 35 is driven by the transmission mechanism 5 to extend into the upper part of the inner cavity of the mounting shell 32. Under the interaction of the spiral groove 66 and the guide rod 65, the driving rod 63 can be rotated. The rotation of the driving rod 63 can drive the cotton rolling stick 35 to perform circumferential motion and rotation to wind the cotton ball.

[0045] like Figure 5 and Fig.10 As shown, the driving mechanism 6 also includes a driving gear 67 fixedly sleeved on the bottom of the driving rod 63, a gear ring 68 fixedly sleeved on the upper part of the outer surface of the annular block 34 and meshing with the driving gear 67, and a roller 69 movably installed on the inner ring of the annular block 34 through a bearing; the inner ring of the roller 69 is key-connected with the outer surface of the cotton rolling rod 35, and the outer ring of the roller 69 is movably connected with the bottom surface of the supporting plate 31.

[0046] When in use, when the driving rod 63 rotates, the annular block 34 can be driven to rotate through the driving gear 67 and the ring gear 68. When the annular block 34 rotates, it can drive the cotton rolling rod 35 to move circumferentially. Under the interaction between the roller 69 and the bottom surface of the supporting plate 31, the cotton rolling rod 35 can rotate while moving circumferentially.

[0047] like Fig.11 As shown, the driving mechanism 6 also includes a group of mounting grooves equidistantly arranged on the bottom surface of the assembly block 64 in the circumferential direction, and a clamping plate 610 movably connected to the outer surface of the chromatography column 2 and a screw 611 threadedly installed on the outer ring of the assembly block 64 are installed in the inner cavity of the mounting groove for transverse sliding; one end of the screw 611 passes through the inner cavity of the connecting shell 61, and one end of the screw 611 is movably connected to the outer surface of the driving rod 63, and the clamping plate 610 is movably connected to the screw 611 through a bearing.

[0048] When in use, after the material taking mechanism 3 is inserted into the chromatography column 2, the clamping plate 610 can be driven to move and fit and squeeze the outer wall of the chromatography column 2 by rotating the screw 611. At the same time, one end of the screw 611 can squeeze the driving rod 63, so as to facilitate the limiting and fixing of the material taking mechanism 3 inserted into the chromatography column 2.

[0049] like Figure 4 and Fig. 9 As shown, the transmission mechanism 5 includes a mounting channel equidistantly arranged at the lower part of the mounting shell 32, a pin rod 51 hinged in the inner cavity of the mounting channel, vertical grooves 52 equidistantly arranged on the outer surface of the filter shell 33, and a pin shell 53 movably sleeved on one side of the outer surface of the cotton rolling rod 35 through a bearing; one end of the pin rod 51 is movably connected to the top surface of the inner cavity of the vertical groove 52, and the other end of the pin rod 51 is movably connected to the inner cavity of the pin shell 53, and a first torsion spring for driving the pin rod 51 to rotate is arranged between the pin rod 51 and the inner cavity of the mounting channel.

[0050] When in use, when the filter shell 33 moves downward, it can drive the top surface of the vertical groove 52 to act on one end of the pin rod 51, so that the pin rod 51 can rotate and drive the pin shell 53 to move. The movement of the pin shell 53 can drive the cotton rolling rod 35 to extend into the inner cavity of the mounting shell 32. When the filter shell 33 moves upward, under the drive of the elastic force of the first torsion spring, the pin rod 51 can drive the cotton rolling rod 35 to retract and reset.

[0051] like Fig. 9 As shown, a group of grooves are equidistantly provided on one side of the cotton rolling rod 35 close to the mounting shell 32, a scraper rod 7 is hinged in the inner cavity of the groove, and a second torsion spring for driving the scraper rod 7 to rotate is provided between the scraper rod 7 and the inner cavity of the groove.

[0052] When in use, after the cotton rolling rod 35 is inserted into the inner cavity of the mounting shell 32, the scraper rod 7 can be flipped and erected under the elastic force of the second torsion spring. When the cotton rolling rod 35 rotates, it drives the scraper rod 7 to move and roll the cotton ball, thereby improving the winding effect and efficiency.

[0053] like Figure 6 and Figure 8 As shown, the cleaning mechanism 4 includes a mounting rod 41 slidably mounted on the outer end of the cotton rolling swab 35, an annular liquid storage shell 42 is movably mounted on the outer surface of a group of mounting rods 41 through a bearing, a group of cleaning plates 43 for scraping and cleaning the residues attached to the inner cavity of the chromatography column 2 are fixedly mounted at equal intervals on the outer circle of the liquid storage shell 42, and a group of pressure nozzles 44 for spraying cleaning agent on the inner cavity of the chromatography column 2 are arranged on the outer surface of the cleaning plate 43; the inner cavity of the pressure nozzle 44 is connected to the inner cavity of the liquid storage shell 42, and a spiral sheet is fixedly sleeved on the outer surface of the mounting rod 41. When the mounting rod 41 rotates, the spiral sheet is driven to rotate to spray the cleaning agent in the liquid storage shell 42 through the pressure nozzle 44.

[0054] When in use, the inner cavity of the liquid storage shell 42 is filled with cleaning liquid. When the cotton swab 35 moves and rotates circumferentially, it can drive the mounting rod 41 to move and rotate circumferentially. The mounting rod 41 can drive the liquid storage shell 42 to drive the cleaning plate 43 to move circumferentially to scrape and clean the inner cavity of the chromatography column 2. At the same time, when the mounting rod 41 rotates, under the action of the spiral blade, the cleaning agent in the liquid storage shell 42 can be sprayed out through the pressure nozzle 44 to the inner wall of the chromatography column 2 for cleaning, so that the residue attached to the inner cavity of the chromatography column 2 can be cleaned while taking the material, which is convenient and quick.

[0055] The working principle and use process of the present invention:

[0056] When in use, the operator first presses the assembly block 64 to fit it with the upper surface of the chromatography column 2, and lifts the connecting block 62 upward. During the lifting process, the upper part of the filter shell 33 is moved to the lower part of the inner cavity of the mounting shell 32 under the drive of the spring compression rod 37. When the filter shell 33 moves downward, it can drive the top surface of the vertical groove 52 to act on one end of the pin rod 51, so that the pin rod 51 can rotate and drive the pin shell 53 to move. The movement of the pin shell 53 can drive the cotton rolling rod 35 to extend into the inner cavity of the mounting shell 32. After the cotton rolling rod 35 extends into the inner cavity of the mounting shell 32, the scraper rod 7 can be flipped and erected under the elastic force of the second torsion spring. At the same time, during the lifting process, the interaction between the spiral groove 66 and the guide rod 65 can make the driving rod 63 rotate. When the driving rod 63 rotates, the annular block 34 can be driven through the driving gear 67 and the gear ring 68. Rotation, when the annular block 34 rotates, it can drive the cotton rolling stick 35 to move circumferentially. Under the interaction between the roller 69 and the bottom surface of the supporting plate 31, the cotton rolling stick 35 can rotate while moving circumferentially, and can roll and collect the cotton balls on the supporting plate 31, and at the same time, break the sand blocks above the cotton balls, so that the sand particles fall into the collection shell 36 through the discharge channel 38 for collection, and when the cotton rolling stick 35 moves and rotates circumferentially, it can drive the mounting rod 41 to move and rotate circumferentially, and the mounting rod 41 can drive the liquid storage shell 42 to drive the cleaning plate 43 to move circumferentially to scrape and clean the inner cavity of the chromatography column 2. At the same time, when the mounting rod 41 rotates, under the action of the spiral blade, the cleaning agent in the liquid storage shell 42 can be sprayed out through the pressure nozzle 44 to the inner wall of the chromatography column 2 for cleaning, so as to clean the residues attached to the inner cavity of the chromatography column 2 while taking the material.

[0057] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A protein separation and detection device, characterized in that: The invention comprises a detector (1), wherein a chromatography column (2) is vertically arranged above the detector (1), a bottom liquid outlet of the chromatography column (2) is connected to a liquid inlet port of the detector (1) via a hose, and a material taking mechanism (3) for taking out cotton balls and sand particles is arranged in the inner cavity of the chromatography column (2); The material taking mechanism (3) comprises a supporting plate (31) placed at the lower part of the inner cavity of the chromatography column (2) for supporting the cotton balls, an annular mounting shell (32) is movably mounted in the inner cavity of the supporting plate (31) via a bearing, a filter shell (33) capable of moving up and down is arranged in the inner cavity of the mounting shell (32), an annular block (34) is fixedly mounted on the outer ring of the mounting shell (32) via a bracket, and a group of equidistant inner circumferences of the inner cavity of the annular block (34) are arranged capable of extending into the inner cavity of the mounting shell (32) for winding the cotton balls. A cotton rolling stick (35), wherein a cleaning mechanism (4) for cleaning the inner cavity of the chromatography column (2) is arranged at one end of the cotton rolling stick (35) away from the mounting shell (32), a transmission mechanism (5) is arranged below the cotton rolling stick (35), a collection shell (36) is threadedly mounted on the lower part of the outer surface of the mounting shell (32), the bottom surface of the collection shell (36) is movably connected to the bottom surface of the inner cavity of the chromatography column (2), and a driving mechanism (6) for driving the cotton rolling stick (35) to rotate is arranged on the upper surface of the supporting plate (31); The bottom surface of the filter shell (33) is movably connected to the bottom surface of the inner cavity of the chromatography column (2); a group of spring compression rods (37) for driving the filter shell (33) to move downward are equidistantly arranged in the inner cavity of the mounting shell (32); when the filter shell (33) moves downward to the lower part of the inner cavity of the mounting shell (32), the cotton rolling rod (35) can be driven by the transmission mechanism (5) to extend into the upper part of the inner cavity of the mounting shell (32); and a group of discharge channels (38) are equidistantly arranged in the middle part of the inner cavity of the mounting shell (32); the driving mechanism (6) comprises a group of connecting shells (61) vertically fixedly mounted on the upper surface of the supporting plate (31); ), the outer wall of the connecting shell (61) is movably connected to the inner cavity of the chromatography column (2), the top surface of the connecting shell (61) is fixedly installed with an annular connecting block (62), the bottom surface of the connecting block (62) is located in the inner cavity of the connecting shell (61) and is rotatably installed with a driving rod (63) for driving the cotton rolling stick (35) to rotate, a group of annular assembly blocks (64) are arranged on the outer side of the connecting shell (61), the bottom surface of the assembly block (64) is movably connected to the upper surface of the chromatography column (2), the inner ring bearing of the assembly block (64) is equidistantly fixedly installed with a group of assembly blocks (64) extending into the inner cavity of the connecting shell (61), the assembly blocks are rotatably connected to the The inner cavity of the connecting shell (61) is slidably connected, a guide rod (65) is fixedly installed on the outer surface of the assembly block (64), a spiral groove (66) is circumferentially opened on the outer surface of the driving rod (63), and the guide rod (65) is movably connected to the inner cavity of the spiral groove (66); the driving mechanism (6) also includes a driving gear (67) fixedly sleeved on the bottom of the driving rod (63), a gear ring (68) fixedly sleeved on the upper part of the outer surface of the annular block (34) and meshing with the driving gear (67), and a roller (69) movably installed on the inner ring of the annular block (34) through a bearing; the inner ring of the roller (69) is in contact with the outer surface of the cotton rolling stick (35). The outer ring of the roller (69) is movably connected to the bottom surface of the support plate (31); the driving mechanism (6) also includes a group of mounting grooves equidistantly arranged on the bottom surface of the assembly block (64) in the circumferential direction, and a clamping plate (610) movably connected to the outer surface of the chromatography column (2) and a screw (611) threadedly installed on the outer ring of the assembly block (64) are installed in a transverse sliding manner in the inner cavity of the mounting groove; one end of the screw (611) passes through the inner cavity of the connecting shell (61), and one end of the screw (611) is movably connected to the outer surface of the driving rod (63), and the clamping plate (610) is movably connected to the screw (611) through a bearing;The transmission mechanism (5) comprises an installation channel equidistantly arranged at the bottom of the installation shell (32), a pin rod (51) hinged in the inner cavity of the installation channel, vertical grooves (52) equidistantly arranged on the outer surface of the filter shell (33), and a pin shell (53) movably sleeved on one side of the outer surface of the cotton rolling rod (35) through a bearing; one end of the pin rod (51) is movably connected to the top surface of the inner cavity of the vertical groove (52), the other end of the pin rod (51) is movably connected to the inner cavity of the pin shell (53), and a first torsion spring for driving the pin rod (51) to rotate is arranged between the pin rod (51) and the inner cavity of the installation channel; a group of grooves equidistantly arranged on one side of the cotton rolling rod (35) close to the installation shell (32), a scraper rod (7) is hinged in the inner cavity of the groove, and a spring for driving the scraper rod (7) to rotate is arranged between the scraper rod (7) and the inner cavity of the groove. The second torsion spring for rotating the scraper rod (7); the cleaning mechanism (4) comprises a mounting rod (41) slidably mounted on the outer end of the cotton rolling rod (35); a ring-shaped liquid storage shell (42) is movably mounted on the outer surface of the mounting rod (41) through a bearing; a group of cleaning plates (43) for scraping and cleaning the residues attached to the inner cavity of the chromatography column (2) are fixedly mounted on the outer ring of the liquid storage shell (42) at equal intervals in the circumferential direction; a group of pressure nozzles (44) for spraying cleaning agent on the inner cavity of the chromatography column (2) are arranged on the outer surface of the cleaning plate (43); the inner cavity of the pressure nozzle (44) is connected to the inner cavity of the liquid storage shell (42); a spiral sheet is fixedly sleeved on the outer surface of the mounting rod (41); when the mounting rod (41) rotates, the spiral sheet is driven to rotate to spray the cleaning agent in the liquid storage shell (42) through the pressure nozzle (44). ; 2. A protein separation and detection device according to claim 1, characterized in that: A ""-shaped bracket is vertically fixedly mounted on the side wall of the detector (1), and the chromatography column (2) is detachably connected to the upper right side of the bracket.

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