A earthworm protein composition capable of dissolving blood clots and repairing blood vessels and a preparation method thereof

Abstract

The present invention provides a thrombus-dissolving and blood vessel-repairing earthworm protein composition and a preparation method thereof. The present invention modifies earthworm protein, the main raw material of the earthworm protein composition, and simultaneously adds a quercetin complex and a curcumin complex, so that the three synergistically exert their effects, so that after taking the earthworm protein composition, the consumer can achieve a better thrombus-dissolving effect and an improved blood vessel-repairing effect to a certain extent.

Description

Technical Field

[0001] The invention belongs to the field of earthworm protein compositions, and in particular relates to an earthworm protein composition capable of dissolving thrombus and repairing blood vessels and a preparation method thereof. Background Art

[0002] Earthworms, also known as earthworms, are the dried bodies of the earthworms of the family Lumbricidae. They are cold in nature, salty in taste, and have anti-inflammatory, analgesic, blood circulation and blood stasis effects. Their active ingredient, earthworm protein, is an active protein containing nucleic acids, fibrinolytic enzymes and other components. Earthworm protein has a wide range of effects on the body's coagulation and fibrinolytic systems, and can significantly reduce platelet adhesion, increase cerebral blood flow, and reduce cerebral vascular resistance.

[0003] Thrombosis refers to a solid mass formed by coagulation in the living vascular system with the participation of red blood cells, platelets and fibrin and other components due to the slowing of blood flow or the generation of vortices, changes in blood properties and damage to vascular endothelial cells; thrombus can block blood flow to various organs and tissues in the body, and is one of the causes of cardiovascular disease, leading to myocardial infarction, stroke and pulmonary embolism, thereby increasing mortality. The existing earthworm protein composition has a low efficacy only by its own action, has poor thrombus-dissolving effect, and has a weak ability to repair blood vessels, which cannot meet the current demand for earthworms for vascular diseases.

[0004] Therefore, how to develop a composite earthworm protein composition for improving thrombosis, which can both dissolve thrombus and efficiently repair blood vessels, is a problem that needs to be solved urgently. Summary of the invention

[0005] In view of the deficiencies in the prior art, the purpose of the present invention is to provide a earthworm protein composition that can dissolve blood clots and repair blood vessels and a preparation method thereof, aiming to solve the problems that the existing earthworm protein composition has low therapeutic effect only by relying on its own effect, has poor effect on dissolving blood clots, and has weak ability to repair blood vessels.

[0006] The present invention modifies the earthworm protein, which is the main raw material of the earthworm protein composition, and adds a quercetin complex and a curcumin complex at the same time. Through the synergistic effect of the three, after taking the earthworm protein composition, the consumer can achieve better thrombus dissolving effect and enhanced blood vessel repair effect to a certain extent.

[0007] The technical solution of the present invention is as follows:

[0008] The first aspect of the present invention provides a method for preparing a composition of earthworm protein capable of dissolving thrombus and repairing blood vessels, characterized in that the method comprises the following steps:

[0009] Step S1: weighing raw materials according to weight parts: 30-40 parts of earthworm protein, 20-30 parts of isomalt, 20-30 parts of maltodextrin, 15-25 parts of natto powder, 20-25 parts of quercetin complex, 15-20 parts of curcumin complex, and 7-14 parts of food additives;

[0010] Step S2: drying, crushing and passing the raw materials in step S1 through an 80-mesh sieve to obtain fine powder;

[0011] Step S3: Mix the fine powder in step S2 in a high-speed mixer for 10-20 minutes, add deionized water to the uniformly mixed powder, perform wet granulation, and pass through a 20-mesh sieve to obtain wet granules;

[0012] Step S4: placing the wet granules in step S3 into a dryer and drying until the moisture content of the granules is less than 4%, taking out the granules, cooling them to room temperature, and passing them through a 20-mesh sieve to obtain dry granules;

[0013] Step S5: Add 3-6 parts of magnesium stearate to the dry particles in step S4, mix for 0.5 hours, and compress into tablets to prepare a earthworm protein composition that can dissolve blood clots and repair blood vessels.

[0014] The following are preferred technical solutions of the present invention, but are not intended to limit the technical solutions provided by the present invention. Through the following preferred technical solutions, the objectives and beneficial effects of the present invention can be better achieved and realized.

[0015] As a preferred technical solution of the present invention, the earthworm protein is a modified earthworm protein; the preparation of the modified earthworm protein includes the following steps: 10 parts of earthworm protein, 0.5 parts of bromelain, and 100 parts of deionized water are mixed evenly, and hydrolyzed at a temperature of 45°C for 1 hour to obtain a hydrolyzate; then 4 parts of streptokinase and 6 parts of oligofructose are added and mixed evenly, and the mixture is reacted at a temperature of 100°C for 0.5 hour to obtain a reaction solution; after the reaction solution is naturally cooled to room temperature, it is reduced pressure concentrated at a vacuum degree of 0.09 MPa and a temperature of 65°C for 5 hours to obtain a concentrated solution; the concentrated solution is vacuum freeze-dried to obtain the modified earthworm protein.

[0016] Preferably, the pre-freezing temperature of the vacuum freeze drying is set to -26 to -24°C, and when the temperature drops to the pre-freezing temperature, it is maintained for 1.5 to 2.5 hours, the sublimation temperature is set to 8 to 12°C, the decomposition temperature is set to 25 to 35°C, the vacuum degree is 10 to 20 Pa, and the drying time is 18 to 22 hours.

[0017] Earthworm protein is rich in fibrinolytic enzymes, which can act on the fibrin in the blood and degrade it into small soluble fragments. These fragments are easier to decompose and clear in the blood circulation, thereby achieving the thrombolytic function; the plasminogen activator streptokinase can combine with free fibrinogen to form compounds, which activate the formation of plasminogen activators to exert their effects. Earthworm protein and the activator work together to achieve the effect of dissolving blood clots.

[0018] As a preferred technical solution of the present invention, the preparation of the quercetin complex includes the following steps: mixing 10 to 15 parts of quercetin and 2 to 4 parts of zein, stirring at 1200 to 1500 r / min for 0.5 to 1 hour, then adding 2 to 4 parts of tanshinone and 1 to 3 parts of polyethylene glycol, ultrasonically treating for 1 hour, and obtaining the quercetin complex after drying.

[0019] Quercetin is a protein disulfide isomerase inhibitor that can inhibit collagen-induced platelet aggregation, reduce platelet activity, delay thrombus growth and dissolve thrombus by blocking the exchange reaction of disulfide bonds and thiol groups of proteins inside and outside cells. Danshensu can improve cell proliferation, migration, differentiation and adhesion functions, promote the secretion of cytokines, and thus promote the repair of endothelium and blood vessels.

[0020] As a preferred technical solution of the present invention, the preparation of the curcumin complex includes the following steps: adding 4 to 6 parts of konjac flour, 2 to 4 parts of egg white protein, and 2 to 4 parts of rutin to 300 parts of deionized water, stirring evenly, and then adding 5 to 10 parts of curcumin, reacting for 0.5 to 1 hour at a stirring rate of 100-300 rpm, and spray drying to obtain the curcumin complex.

[0021] Curcumin can act on cells, promote the secretion of cytokines such as vascular endothelial growth factor, promote angiogenesis in ischemic tissues, and participate in the re-endothelialization and vascular repair of damaged blood vessels; rutin, as an anti-thrombotic compound, has an inhibitory effect on platelet accumulation and fibrin production during thrombosis, and can treat platelet-rich and fibrin-rich thrombi to achieve the effect of dissolving thrombi.

[0022] Preferably, the food additives include: 3-6 parts of microcrystalline cellulose, 1-2 parts of vitamin C, 3-6 parts of sodium citrate, and 3-6 parts of magnesium stearate.

[0023] Preferably, the drying temperature in step S4 is 50-60° C., and the drying time is 40-50 min.

[0024] In a second aspect, the present invention provides a composition of earthworm protein that can dissolve blood clots and repair blood vessels and is prepared by the method described in the first aspect.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] (1) The present invention modifies earthworm protein and introduces a fibrinolytic enzyme activator. On the basis of the original fibrinolytic enzyme in the earthworm protein, the activator combines with free fibrinogen to form a compound, thereby increasing the amount of fibrinolytic enzyme and achieving the purpose of efficient activation. The earthworm protein and the activator in the modified earthworm protein synergistically achieve the effect of dissolving blood clots.

[0027] (2) The present invention adds quercetin complex and curcumin complex to the earthworm protein composition, and works together with the modified earthworm protein to reduce platelet activity, delay thrombus growth and dissolve thrombus, and participate in re-endothelialization and vascular repair of damaged blood vessels, thereby synergistically achieving the technical effects of dissolving thrombus and efficiently repairing blood vessels. DETAILED DESCRIPTION

[0028] For the convenience of understanding the present invention, the present invention lists the following embodiments. It should be understood by those skilled in the art that the embodiments are only to help understand the present invention and should not be regarded as specific limitations of the present invention.

[0029] The sources of some components in the embodiments and comparative examples are as follows:

[0030] Earthworm protein: purchased from Runfulai Biotechnology Nanjing Co., Ltd.;

[0031] Isomalt: CAS number is 64519-82-0;

[0032] Maltodextrin: CAS number is 9050-36-6;

[0033] Natto powder: purchased from Xi'an Anao Biotechnology Co., Ltd.;

[0034] Food additives: The CAS number of microcrystalline cellulose is 9004-34-6, the CAS number of vitamin C is 50-81-7, and the CAS number of sodium citrate is 68-04-2;

[0035] Magnesium stearate: CAS number is 557-04-0.

[0036] Preparation of modified earthworm protein: 10 parts of earthworm protein (purchased from Runfulai Biotechnology Nanjing Co., Ltd.), 0.5 parts of bromelain (CAS No. 9001-00-7), and 100 parts of deionized water were mixed evenly, and hydrolyzed at a temperature of 45°C for 1 hour to obtain a hydrolyzate; then 4 parts of streptokinase (CAS No. 9002-01-1) and 6 parts of oligofructose (CAS No. 308066-66-2) were added and mixed evenly, and reacted at a temperature of 100°C for 0.5 hour to obtain a reaction solution; after the reaction solution was naturally cooled to room temperature, it was concentrated under reduced pressure at a vacuum degree of 0.09 MPa and a temperature of 65°C for 5 hours to obtain a concentrated solution; the concentrated solution was vacuum freeze-dried, the pre-freezing temperature was set to -24°C, and when the temperature dropped to the pre-freezing temperature, it was kept for 1.5 hours, the sublimation temperature was set to 8°C, the decomposition temperature was set to 25°C, the vacuum degree was 10Pa, and the drying time was 22 hours to obtain modified earthworm protein.

[0037] Preparation of quercetin complex: 15 parts of quercetin (CAS No. 117-39-5) and 4 parts of zein (CAS No. 9010-66-6) were mixed, stirred at 1500 r / min for 1 hour, and then 4 parts of tanshinone (CAS No. 76822-21-4) and 3 parts of polyethylene glycol (CAS No. 25322-68-3) were added, ultrasonically treated for 1 hour, and dried to obtain the quercetin complex.

[0038] Preparation of curcumin complex: 4 parts of konjac flour (CAS No. 37220-17-0), 2 parts of egg white protein (CAS No. 37220-17-0), and 4 parts of rutin (CAS No. 153-18-4) were added to 300 parts of deionized water, stirred evenly, and then 10 parts of curcumin (CAS No. 458-37-7) were added, and the mixture was reacted at a stirring rate of 300 rpm for 1 hour, and the curcumin complex was obtained after spray drying.

[0039] Example 1

[0040] This embodiment provides a method for preparing a pithworm protein composition capable of dissolving thrombus and repairing blood vessels, the preparation method comprising the following steps:

[0041] Step S1: weighing raw materials according to weight parts: 35 parts of modified earthworm protein, 25 parts of isomalt, 25 parts of maltodextrin, 20 parts of natto powder, 22 parts of quercetin complex, 18 parts of curcumin complex, 5 parts of microcrystalline cellulose, 1.5 parts of vitamin C, and 5 parts of sodium citrate;

[0042] Step S2: drying, crushing and passing the raw materials in step S1 through an 80-mesh sieve to obtain fine powder;

[0043] Step S3: Mix the fine powder in step S2 in a high-speed mixer for 15 minutes, add deionized water to the uniformly mixed powder, perform wet granulation, and pass through a 20-mesh sieve to obtain wet granules;

[0044] Step S4: placing the wet granules in step S3 into a dryer and drying until the moisture content of the granules is 3.8%, taking out the granules, cooling them to room temperature, and passing them through a 20-mesh sieve to obtain dry granules;

[0045] Step S5: Add 5 parts of magnesium stearate to the dry particles in step S4, mix for 0.5 hours, and tablet, to prepare a earthworm protein composition capable of dissolving blood clots and repairing blood vessels.

[0046] Example 2

[0047] This embodiment provides a method for preparing a pithworm protein composition capable of dissolving thrombus and repairing blood vessels, the preparation method comprising the following steps:

[0048] Step S1: weighing raw materials according to weight parts: 30 parts of modified earthworm protein, 20 parts of isomalt, 20 parts of maltodextrin, 15 parts of natto powder, 20 parts of quercetin complex, 15 parts of curcumin complex, 3 parts of microcrystalline cellulose, 1 part of vitamin C, and 3 parts of sodium citrate;

[0049] Step S2: drying, crushing and passing the raw materials in step S1 through an 80-mesh sieve to obtain fine powder;

[0050] Step S3: Mix the fine powder in step S2 in a high-speed mixer for 10 minutes, add deionized water to the uniformly mixed powder, perform wet granulation, and pass through a 20-mesh sieve to obtain wet granules;

[0051] Step S4: placing the wet granules in step S3 into a dryer and drying until the moisture content of the granules is 3.6%, taking out the granules, cooling them to room temperature, and passing them through a 20-mesh sieve to obtain dry granules;

[0052] Step S5: Add 3 parts of magnesium stearate to the dry particles in step S4, mix for 0.5 hours, and tablet, to prepare a earthworm protein composition capable of dissolving blood clots and repairing blood vessels.

[0053] Example 3

[0054] This embodiment provides a method for preparing a pithworm protein composition capable of dissolving thrombus and repairing blood vessels, the preparation method comprising the following steps:

[0055] Step S1: weighing raw materials in parts by weight according to 40 parts of modified earthworm protein, 30 parts of isomalt, 30 parts of maltodextrin, 25 parts of natto powder, 25 parts of quercetin complex, 20 parts of curcumin complex, 6 parts of microcrystalline cellulose, 2 parts of vitamin C, and 6 parts of sodium citrate;

[0056] Step S2: drying, crushing and passing the raw materials in step S1 through an 80-mesh sieve to obtain fine powder;

[0057] Step S3: Mix the fine powder in step S2 in a high-speed mixer for 20 minutes, add deionized water to the uniformly mixed powder, perform wet granulation, and pass through a 20-mesh sieve to obtain wet granules;

[0058] Step S4: placing the wet granules in step S3 into a dryer and drying until the moisture content of the granules is 3.9%, taking out the granules, cooling them to room temperature, and passing them through a 20-mesh sieve to obtain dry granules;

[0059] Step S5: Add 6 parts of magnesium stearate to the dry particles in step S4, mix for 0.5 hours, and compress into tablets to prepare a earthworm protein composition that can dissolve blood clots and repair blood vessels.

[0060] Example 4

[0061] This embodiment provides a method for preparing a composition of earthworm protein that can dissolve blood clots and repair blood vessels. The only difference from Example 1 is that this embodiment replaces the modified earthworm protein with earthworm protein (purchased from Runfulai Biotechnology Nanjing Co., Ltd.), and the rest is the same as Example 1.

[0062] Example 5

[0063] This embodiment provides a method for preparing a composition of earthworm protein that can dissolve blood clots and repair blood vessels. The only difference from Example 1 is that no quercetin complex is added to this embodiment, and the rest is the same as Example 1.

[0064] Example 6

[0065] This embodiment provides a method for preparing a pith protein composition that can dissolve blood clots and repair blood vessels. The only difference from Example 1 is that no curcumin complex is added in this embodiment, and the rest is the same as Example 1.

[0066] Comparative Example 1

[0067] This comparative example provides a method for preparing a ground dragon protein composition, the preparation method comprising the following steps:

[0068] Step S1: weighing raw materials in parts by weight according to 35 parts of earthworm protein, 25 parts of isomalt, 25 parts of maltodextrin, 20 parts of natto powder, 5 parts of microcrystalline cellulose, 1.5 parts of vitamin C, and 5 parts of sodium citrate;

[0069] Step S2: drying, crushing and passing the raw materials in step S1 through an 80-mesh sieve to obtain fine powder;

[0070] Step S3: Mix the fine powder in step S2 in a high-speed mixer for 15 minutes, add deionized water to the uniformly mixed powder, perform wet granulation, and pass through a 20-mesh sieve to obtain wet granules;

[0071] Step S4: placing the wet granules in step S3 into a dryer and drying until the moisture content of the granules is 3.8%, taking out the granules, cooling them to room temperature, and passing them through a 20-mesh sieve to obtain dry granules;

[0072] Step S5: Add 5 parts of magnesium stearate to the dry particles in step S4, mix for 0.5 hours, and tablet, to prepare a earthworm protein composition.

[0073] Comparative Example 2

[0074] This comparative example provides a method for preparing a ground dragon protein composition, which is different from Comparative Example 1 only in that the ground dragon protein (purchased from Runfulai Biotechnology Nanjing Co., Ltd.) is replaced by modified ground dragon protein in this comparative example.

[0075] Comparative Example 3

[0076] This comparative example provides a method for preparing a groundworm protein composition, which is different from comparative example 1 only in that 22 portions of quercetin complex are added in this comparative example.

[0077] Comparative Example 4

[0078] This comparative example provides a method for preparing a ground dragon protein composition, which is different from comparative example 1 only in that 18 portions of curcumin complex are added in this comparative example.

[0079] The effects of the earthworm protein compositions provided in the above embodiments and comparative examples were tested as follows:

[0080] Efficacy test of rat carotid artery model: SD rats were divided into 8 groups, 8 rats in each group. Fasting for 12h, intraperitoneal injection of 10% chloral hydrate 350mg / kg anesthesia, neck hair removed, iodine tincture fully disinfected, and skin incision of about 3cm long. Separate the carotid artery and fully expose it. Soak 0.5×0.5cm filter paper in 30% ferric chloride solution for 30s and wrap the carotid artery. Place a tin foil pad under the filter paper to separate the filter paper from the surrounding tissue to prevent ferric chloride from damaging the surrounding tissue. The sham operation group was treated with filter paper soaked in saline. After 3min, the filter paper was removed and the wrapped artery was rinsed twice with saline, the wound was sutured, and the iodine tincture was disinfected. One day after the operation, 1.5g of the earthworm protein composition provided in the embodiment and the comparative example was weighed, dissolved in saline and gavaged with rats, and the control group was gavaged with an equal amount of saline, once a day, for 4 consecutive weeks.

[0081] 1. Thrombolysis test

[0082] One day after intragastric administration, 0.5 cm of the carotid artery at the surgical site was cut, and excess water was absorbed by filter paper and weighed to evaluate the thrombolytic effect. The test results are shown in Table 1.

[0083] Table 1 Thrombolysis test results

[0084] Thrombus weight (g) Sham operation group 4.86±0.52 Control group 13.72±1.26 Experimental Group 1 (Example 1) 6.95±0.44 Experimental Group 2 (Example 2) 6.98±0.48 Experimental Group 3 (Example 3) 6.91±0.42 Experimental Group 4 (Example 4) 8.34±0.75 Experimental Group 5 (Example 5) 8.64±0.91 Experimental Group 6 (Example 6) 8.71±0.92 Experimental group 7 (comparative example 1) 11.72±1.12 Experimental Group 8 (Comparative Example 2) 9.57±1.03 Experimental Group 9 (Comparative Example 3) 9.82±1.06 Experimental group 10 (comparative example 4) 9.89±1.08

[0085] 2. Vascular repair testing

[0086] One day after the end of intragastric administration, the carotid artery at the surgical site was cut to a length of 2 cm, fixed with 4% paraformaldehyde neutral PBS buffer, dehydrated, embedded in paraffin, and sliced. Each slice was about 5 μm thick. SABC method CD31 immunohistochemical staining was performed according to the instructions of the immunohistochemical kit; DAB color development. All single endothelial cells or endothelial cell clusters stained brown were counted as a blood vessel, and all blood vessels with a lumen larger than 8 red blood cells and a thicker muscle layer were not counted. The blood vessel count was performed according to the Weidner method. First, the high blood vessel density area was found under the low-power (×40) field of view, and then the microvessel count was performed under the high-power (×400) field of view. Ten high-power fields were randomly selected for each slice, and the average value was finally taken as the microvessel density (MVD) value of the specimen. The image analysis was performed using a fully automatic color image processing system to determine the vascular field area ratio (positive target area / total statistical field area). The test results are shown in Table 2.

[0087] Table 2 Vascular repair test results

[0088] Vascular field area (‰) Sham operation group 24.4±2.7 Control group 25.2±2.3 Experimental Group 1 (Example 1) 38.3±2.2 Experimental Group 2 (Example 2) 38.1±2.1 Experimental Group 3 (Example 3) 38.4±2.2 Experimental Group 4 (Example 4) 37.7±2.4 Experimental Group 5 (Example 5) 33.7±2.5 Experimental Group 6 (Example 6) 33.4±2.4 Experimental group 7 (comparative example 1) 25.4±2.2 Experimental group 8 (comparative example 2) 25.5±2.1 Experimental Group 9 (Comparative Example 3) 29.8±2.3 Experimental group 10 (comparative example 4) 29.5±2.4

[0089] From the above content, it can be seen that the present invention modifies the main raw material of the earthworm protein composition, and adds quercetin complex and curcumin complex at the same time, and the three work together to exert the effect. The weight of thrombus in experimental group 1 was 6.95±0.44g, and the vascular field area was 38.3±2.2‰; the weight of thrombus in experimental group 2 was 6.98±0.48g, and the vascular field area was 38.1±2.1‰; the weight of thrombus in experimental group 3 was 6.91±0.42 g, and the vascular field area was 38.4±2.2‰.

[0090] Compared with Example 1, when the modified earthworm protein was replaced with earthworm protein (purchased from Runfulai Biotechnology Nanjing Co., Ltd.), the thrombolytic effect became worse (experimental group 4); compared with Example 1, if the quercetin complex was not added, the thrombolytic effect became worse and the vascular repair effect was not good (experimental group 5); compared with Example 1, if the curcumin complex was not added, the thrombolytic effect became worse and the vascular repair effect was not good (experimental group 6).

[0091] Comparative Example 1 did not adopt the technical innovation of the present invention, and the effective thrombus weight was 11.72±1.12g (experimental group 7); compared with Comparative Example 1, if modified earthworm protein was used, the thrombus dissolving effect was better (experimental group 8); compared with Comparative Example 1, if quercetin complex was added, the thrombus dissolving effect was better and the vascular repair effect was improved (experimental group 9); compared with Comparative Example 1, if curcumin complex was added, the thrombus dissolving effect was better and the vascular repair effect was improved (experimental group 10).

[0092] In summary, the present invention modifies the earthworm protein, the main raw material of the earthworm protein composition, and adds a quercetin complex and a curcumin complex at the same time. Through the synergistic effect of the three, the consumer who takes the earthworm protein composition can achieve better thrombus dissolving effect and enhanced vascular repair effect to a certain extent.

[0093] The applicant declares that the present invention illustrates the detailed process flow of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned detailed process flow, that is, it does not mean that the present invention must rely on the above-mentioned detailed process flow to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

Claims

1. A method for preparing a composition of earthworm protein capable of dissolving blood clots and repairing blood vessels, characterized in that: The following steps are involved: Step S1: weighing raw materials according to weight parts: 30-40 parts of earthworm protein, 20-30 parts of isomalt, 20-30 parts of maltodextrin, 15-25 parts of natto powder, 20-25 parts of quercetin complex, 15-20 parts of curcumin complex, and 7-14 parts of food additives; Step S2: drying, crushing and passing the raw materials in step S1 through an 80-mesh sieve to obtain fine powder; Step S3: Mix the fine powder in step S2 in a high-speed mixer for 10-20 minutes, add deionized water to the uniformly mixed powder, perform wet granulation, and pass through a 20-mesh sieve to obtain wet granules; Step S4: placing the wet granules in step S3 into a dryer and drying until the moisture content of the granules is less than 4%, taking out the granules, cooling them to room temperature, and passing them through a 20-mesh sieve to obtain dry granules; Step S5: adding 3-6 parts of magnesium stearate to the dry particles in step S4, mixing for 0.5 hours, and tableting to prepare a earthworm protein composition capable of dissolving blood clots and repairing blood vessels; The earthworm protein is a modified earthworm protein; The preparation of the modified earthworm protein comprises the following steps: 10 parts of earthworm protein, 0.5 parts of bromelain and 100 parts of deionized water are mixed evenly, and hydrolyzed at a temperature of 45°C for 1 hour to obtain a hydrolyzate; then 4 parts of streptokinase and 6 parts of oligofructose are added and mixed evenly, and reacted at a temperature of 100°C for 0.5 hour to obtain a reaction solution; after the reaction solution is naturally cooled to room temperature, it is reduced pressure concentrated at a vacuum degree of 0.09MPa and a temperature of 65°C for 5 hours to obtain a concentrated solution; the concentrated solution is vacuum freeze-dried to obtain the modified earthworm protein; The preparation of the quercetin complex comprises the following steps: 10-15 parts of quercetin and 2-4 parts of zein are mixed, stirred at 1200-1500 r / min for 0.5-1 h, then 2-4 parts of danshensu and 1-3 parts of polyethylene glycol are added, ultrasonically treated for 1 h, and dried to obtain the quercetin complex; The preparation of the curcumin complex comprises the following steps: adding 4 to 6 parts of konjac flour, 2 to 4 parts of egg white protein and 2 to 4 parts of rutin into 300 parts of deionized water, stirring evenly, adding 5 to 10 parts of curcumin, reacting for 0.5 to 1 hour at a stirring rate of 100 to 300 rpm, and spray drying to obtain the curcumin complex.

2. The method for preparing a earthworm protein composition capable of dissolving thrombus and repairing blood vessels according to claim 1, characterized in that: The food additives include: 3-6 parts of microcrystalline cellulose, 1-2 parts of vitamin C, 3-6 parts of sodium citrate, and 3-6 parts of magnesium stearate.

3. The method for preparing a earthworm protein composition capable of dissolving thrombus and repairing blood vessels according to claim 1, characterized in that: The drying temperature in step S4 is 50-60° C., and the drying time is 40-50 min.

4. A composition of earthworm protein capable of dissolving blood clots and repairing blood vessels, characterized in that: Prepared according to the method according to any one of claims 1 to 3.