A traditional Chinese medicine composition for relieving alcohol intoxication, protecting the liver and preventing hangover and a preparation method thereof

Abstract

The present invention provides a traditional Chinese medicine composition for relieving hangover, protecting the liver and preventing hangovers and a preparation method thereof. The present invention prepares the traditional Chinese medicine composition by means of microbial fermentation, namely the traditional Chinese medicine material Jiuhu paste. The ingredients mainly adopt traditional Chinese medicine materials that are both medicine and food, and the main components include Scutellaria baicalensis, Alisma orientale, Puerariae flos, Hovenia dulcis, etc. The insoluble polysaccharide of Scutellaria baicalensis adopted in the present invention is beneficial to preventing alcoholic liver injury and its alcoholic metabolic disorders, and the crude extract of puerarin is used to treat ethanol-induced metabolic abnormalities and liver injury, and can prevent and treat alcohol poisoning. The preparation process includes processes such as soaking, decocting and boiling, and a biological fermentation process is added. The finally prepared traditional Chinese medicine material Jiuhu paste not only has the functions of relieving hangover and preventing hangovers, but also has the ability to reduce alcohol-induced hepatocyte injury and repair hepatocyte injury and promote hepatocyte regeneration. In particular, the traditional Chinese medicine material obtained when the weight ratio of the crude extract of puerarin to the insoluble polysaccharide of Scutellaria baicalensis in the present invention is 10:1 has the ability to significantly reduce the degree of hepatocyte lesions.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicines for relieving hangover, and relates to a traditional Chinese medicine composition for relieving hangover, protecting the liver and preventing hangover, and particularly relates to a traditional Chinese medicine composition for relieving hangover, protecting the liver and preventing hangover and a preparation method thereof. Background Art

[0002] China has a long history of wine culture. With the continuous acceleration of the modern life rhythm, factors such as great work pressure and many business entertainments have led to an increasing number of drinkers and per capita alcohol consumption in China year by year. The damage caused by excessive drinking to the body has become a common concern of the whole society. Long-term excessive drinking can cause chronic alcoholism, leading to diseases such as hepatitis, fatty liver, and liver cirrhosis, seriously endangering human health. In order to cope with and solve the health problems brought about by excessive drinking, many hangover and liver-protecting products have emerged on the market, such as hangover drugs, traditional Chinese medicine preparations, and health products. Although the above products can play a role in accelerating alcohol metabolism to a certain extent, while the drugs relieve hangover, they will cause different degrees of harm to the liver and kidneys due to the metabolic burden. At the same time, through investigation, there is currently no hangover and liver-protecting related product that is completely made of raw materials of both medicine and food and is formed by probiotic fermentation and transformation. Raw materials of both medicine and food not only have primary metabolites such as carbohydrates, proteins, and vitamins, but also have secondary metabolites such as flavonoids, tannins, terpenoids, steroids and their glycosides, and alkaloids. The secondary metabolites in the raw materials of both medicine and food play a similar regulatory function in regulating physiological functions as that in drugs, but the content is not high, and there are no toxic and side effects after long-term use. Therefore, it is of great significance to select raw materials of both medicine and food as the research object and develop hangover and liver-protecting products by combining biological enzymolysis and microbial fermentation technologies to relieve the damage caused by excessive drinking to the liver.

[0003] Alcoholic liver injury is a common chronic progressive liver disease including five types: alcoholic fatty liver, alcoholic hepatitis, alcoholic liver fibrosis, and alcoholic liver cirrhosis. The main pathogenic factor of alcoholic liver injury is long-term heavy drinking, but its pathogenesis is relatively complex and not yet fully understood, and it may be related to multiple factors such as the toxic effects of alcohol and its metabolites on the liver, oxidative stress, etc. In recent years, the incidence of alcoholic liver injury has been increasing year by year, and it has become the second largest liver disease after viral hepatitis, becoming one of the common diseases seriously endangering public health. Since alcoholic liver injury can be reversed and recovered through appropriate intervention in the early stage, therefore, finding effective methods to improve alcoholic liver injury to prevent the occurrence of alcohol-related liver diseases has become a global consensus. At present, although the clinical experience and treatment research on alcoholic liver injury are increasing continuously, the clinical western medicine treatment still mainly focuses on abstinence from alcohol, nutritional support, and symptomatic treatment, and there is no ideal prevention and treatment drug. Therefore, it is of great significance to study and explore drugs with a protective effect on alcoholic liver injury.

[0004] In view of the need to meet the requirement of accelerating alcohol metabolism to a certain extent, while relieving a hangover without causing different degrees of harm to the liver due to metabolic burden, the present invention provides a traditional Chinese medicine composition for relieving a hangover, protecting the liver and preventing a hangover, namely, Jiu Hu Gao. Summary of the Invention

[0005] In view of the above problems, the present invention provides a traditional Chinese medicine composition for relieving a hangover, protecting the liver and preventing a hangover. The insoluble baicalin polysaccharide adopted by the present invention is beneficial to preventing alcoholic liver injury and its alcoholic metabolic disorders, and the pueraria root extract is used to treat ethanol-induced metabolic abnormalities and liver injury, and can prevent alcohol poisoning. The present invention provides a traditional Chinese medicine composition for relieving a hangover, protecting the liver and preventing a hangover and a preparation method thereof. The specific preparation steps are as follows:

[0006] S1. Streak inoculate Aspergillus niger CICC2160 on a PDA slant, and culture it at 28 °C for 65-75 h until the slant is covered with black spores. Under sterile conditions, pick 1 loop of spores and inoculate them into 10 mL of sterilized 0.85% physiological saline containing 0.1% Tween-80, and shake well to obtain an Aspergillus niger suspension with a cell suspension concentration of 2×10 7 CFU / mL to 6×10 7 CFU / mL; weigh 9-10 g of pueraria root residue with a particle size of 0.4-0.5 mm and 5-6 g of wheat bran, add 10-14 mL of distilled water to obtain an initial solid fermentation medium, and then sterilize the medium in a high-pressure sterilizer at 120-130 °C for 30-40 min for standby. Take 10-12 g of the prepared solid-state fermentation medium in a conical flask, and after sterilization, inoculate 1 mL of the Aspergillus niger suspension. Oscillate the conical flask to make the suspension evenly distributed in the medium as much as possible. After irradiating with an ultraviolet lamp (K = 266 nm, 40 W) for 8 min, ferment at 28 °C in an aerobic environment for 24 h. After the fermentation is completed, take out half of the fermented material and place it in another conical flask. Add 90-100 mL of 70% ethanol solution to each of the two conical flasks containing the fermented material, shake well, and place them in a constant temperature water bath at 80 °C for reflux extraction for 180-200 min. During this period, shake the conical flask every 30 min. Finally, filter the materials in the flask by suction, and the combined filtrate is the crude puerarin extract; in this step, Aspergillus niger ferments pueraria root under the irradiation of an ultraviolet lamp. The puerarin extract obtained after fermentation can accelerate alcohol metabolism and protect the liver, and the puerarin content extracted after fermenting for 24 h after irradiating with an ultraviolet lamp for 8 min is the highest.

[0007] S2. Add absolute ethanol to the Scutellaria baicalensis powder to submerge it, soak and stir for 12 - 14 h, repeat the operation 2 - 4 times, and dry at 40 - 60 °C to obtain defatted Scutellaria baicalensis powder; weigh the dried defatted Scutellaria baicalensis powder, add ultrapure water according to the solid - liquid ratio of 1∶20 (g / mL), extract in a boiling water bath for 2 - 5 h, filter, discard the filtrate, and collect the residue for extraction 2 more times; add 0.5 mol / L NaOH solution to the filter residue according to the solid - liquid ratio of 1∶30 (g / mL), let it stand in a 4 °C refrigerator for 12 - 14 h, centrifuge at 6000 - 8000 r / min for 20 - 30 min, collect the supernatant, add glacial acetic acid to the supernatant to adjust the pH value to 7, centrifuge and collect the colloidal precipitate, wash the precipitate with ultrapure water, and vacuum freeze - dry to obtain insoluble Scutellaria baicalensis polysaccharide; the insoluble Scutellaria baicalensis polysaccharide extracted in this step shortens the hangover time and at the same time helps to reduce the damage of alcohol - induced hepatocytes.

[0008] S3. Inoculate Lactobacillus plantarum on MRS solid medium for slant activation, the culture temperature is 37 °C, and incubate at a constant temperature for 24 h for activation. Then transfer the activated strain to LB medium and perform shaking culture under the same conditions until the middle and late logarithmic growth phase of the strain to obtain Lactobacillus plantarum liquid with a concentration of 10 8 CFU / mL for standby; weigh 100 - 120 parts of each of the Chinese medicinal materials Alisma orientale, stir - fried Atractylodes macrocephala, Talc, Lotus leaf, Pueraria flower, Dark plum, and Tremella fuciformis by weight respectively, and grind them into powdery particles with a size of 0.4 mm - 0.005 mm. After mixing, add 8 - 10 times the weight of water and decoct for 1 - 2 h, then filter. Add 5 times the weight of water to the filter residue and decoct for 1 - 2 h, filter, combine the two filtrates and heat - boil to 1 / 2 of the original volume, which is recorded as 100 parts by weight. Then add 1 - 3 parts of Coptis chinensis, 1 - 3 parts of Codonopsis pilosula, 1 - 3 parts of Pinellia ternata processed with ginger, 0.1 - 3 parts of Glycyrrhiza uralensis, 0.1 - 3 parts of Medicated leaven, 0.5 - 2 parts of Germinated barley, 0.5 - 2 parts of Hovenia dulcis, 0.1 - 2 parts of Dry ginger, 0.1 - 2 parts of Pericarpium citri reticulatae viride, 0.1 - 2 parts of Pericarpium citri reticulatae, 0.1 - 2 parts of Angelica sinensis, 0.1 - 2 parts of melted Yellowish - brown gelatin, 0.1 - 2 parts of Colla corii asini, and 0.1 - 2 parts of isomaltooligosaccharide by weight to the above - decocted liquid with a volume of 1 / 2, add 30 - 35 parts of water and steam for 3 - 4 h, and then inoculate 8 - 10 parts of Lactobacillus plantarum liquid with a concentration of 10 8The Lactobacillus plantarum bacterial liquid at CFU / mL was fermented for 4 to 7 days under aerobic conditions at a temperature of 37°C. The obtained fermentation broth was filtered to remove the supernatant. 30 to 40 parts of the crude puerarin extract prepared in step S1 and 3 to 4 parts of the insoluble scutellaria polysaccharide prepared in step S2 were added to 90 parts of the supernatant and mixed evenly, then heated at 65 to 75°C for 10 h, and then transferred to a container and slowly simmered over low heat for 10 to 12 h until it became solid, which was the traditional Chinese medicine composition for relieving alcohol, protecting the liver and preventing hangover, also called Jiu Hu Gao. The Chinese medicinal materials obtained when the weight ratio of the crude puerarin extract prepared in step S1 to the insoluble scutellaria polysaccharide prepared in step S2 was 10:1 had the ability to significantly reduce the degree of hepatocyte lesions. Preferably, Lactobacillus plantarum CICC22697 was selected.

[0009] Among them, Aspergillus niger CICC2160 was purchased from Beijing Baocang Biotechnology Co., Ltd.; Lactobacillus plantarum CICC22697 was purchased from China National Research Institute of Food and Fermentation Industries Co., Ltd.; MRS solid medium was purchased from Shanghai Chunshi Biotechnology Co., Ltd.; LB medium was purchased from Beijing BioLab Technology Co., Ltd.; melted yellow gelatin was purchased from Shandong Dong'e E-Jiao Co., Ltd.; donkey-hide gelatin was purchased from Shandong Dong'e E-Jiao Co., Ltd.; isomaltooligosaccharide was purchased from Chengdu Wanxiang Hongrun Biotechnology Co., Ltd.

[0010] Preferably: In step S1, 9 g of kudzu root residue with a particle size of 0.4 mm and 5 g of wheat bran were weighed and 10 mL of distilled water was added to obtain the initial solid fermentation medium.

[0011] Preferably: In step S1, the culture medium was sterilized at 120°C for 30 min in a high-pressure sterilizer;

[0012] Preferably: In step S2, anhydrous ethanol was added to the scutellaria powder to immerse the scutellaria powder, soaked and stirred for 12 h, the operation was repeated 2 times, and dried at 40°C;

[0013] Preferably: In step S2, centrifuged at a speed of 6000 r / min for 20 min;

[0014] Preferably: In step S3, the particle size was 0.4 mm;

[0015] Preferably: In step S3, 100 parts of each of the Chinese medicinal materials alisma orientale, stir-fried atractylodes macrocephala, talc, lotus leaf, kudzu flower, dark plum and tremella were weighed by weight;

[0016] Preferably: The Chinese medicinal materials alisma orientale, stir-fried atractylodes macrocephala, talc, lotus leaf, kudzu flower, dark plum, tremella, coptis chinensis, codonopsis pilosula, pinellia ternata, licorice, stir-fried medicated leaven, stir-fried malt, hovenia dulcis, dried ginger, green tangerine peel, dried tangerine peel and angelica sinensis in step S3 were purchased from Haozhou Jinqian Chinese Medicinal Materials Seedlings Co., Ltd.;

[0017] Due to the adoption of the above technical solution, the technical progress achieved by the present invention is as follows:

[0018] 1. The Aspergillus niger of the present invention ferments kudzu root under the irradiation of an ultraviolet lamp. The puerarin extract obtained after fermentation can accelerate alcohol metabolism and protect the liver. After 8 minutes of irradiation with the ultraviolet lamp, the content of fermented puerarin is the highest. The puerarin extract can be used to treat ethanol-induced metabolic abnormalities and liver damage, and can prevent and treat alcohol poisoning.

[0019] 2. The insoluble scutellaria polysaccharide extracted by the present invention shortens the hangover time and helps to reduce the damage of alcohol-induced hepatocytes. The insoluble scutellaria polysaccharide is beneficial to preventing alcoholic liver damage and its alcoholic metabolic disorders.

[0020] 3. The present invention provides a traditional Chinese medicine composition for relieving hangover, protecting the liver and preventing hangovers and its preparation method. The preparation process includes processes such as soaking, decocting and boiling, and a biological fermentation process is added. The finally prepared traditional Chinese medicine materials not only have the effects of relieving hangover and preventing hangovers, but also have the ability to reduce alcohol-induced hepatocyte damage, repair hepatocyte damage and promote hepatocyte regeneration. In addition, the traditional Chinese medicine materials obtained when the weight ratio of the crude puerarin extract prepared by the present invention to the insoluble scutellaria polysaccharide is 10:1 have the ability to significantly reduce the degree of hepatocyte lesions. Description of the Drawings

[0021] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following will briefly introduce the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0022] Figure 1 It is a diagram showing the influence of the fermentation time of the present invention's Example 1 and Comparative Examples 1-6 under the irradiation of an ultraviolet lamp on the extraction of puerarin.

[0023] Figure 2 It is a diagram of the pathological results of the liver tissues of the mice in Group 4 of the embodiments of the present invention.

[0024] Figure 3 It is a diagram of the pathological results of the liver tissues of the mice in Group 19 of the embodiments of the present invention.

[0025] Figure 4 It is a diagram of the pathological results of the liver tissues of the mice in Group 20 of the comparative examples of the present invention.

[0026] Figure 5 It is a diagram of the pathological results of the liver tissues of the mice in Group 21 of the comparative examples of the present invention.

[0027] Figure 6It is the case result diagram of the liver tissues of 22 groups of mice in Comparative Example 2 of the present invention. Detailed implementation manners

[0028] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention clearer and more understandable, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the content of the present invention, and are not used to limit the present invention.

[0029] Example 1

[0030] S1. Streak inoculate Aspergillus niger CICC2160 on a PDA slant. After culturing at 28°C for 65 h, the slant is covered with black spores. Under sterile conditions, pick 1 loop of spores and inoculate them into 10 mL of sterilized 0.85% physiological saline containing 0.1% Tween-80 and shake well to obtain an Aspergillus niger spore suspension with a concentration of 2×10 7 CFU / mL; weigh 9 g of Pueraria lobata residue with a particle size of 0.4 mm and 5 g of wheat bran, add 10 mL of distilled water to obtain the initial solid fermentation medium. Then sterilize the medium in an autoclave at 120°C for 30 min and set aside. Take 10 g of the prepared solid fermentation medium in a conical flask. After sterilization, inoculate 1 mL of the Aspergillus niger spore suspension, shake the conical flask to make the spore suspension evenly distributed in the medium as much as possible. After irradiating with an ultraviolet lamp (K = 266 nm, 40 W) for 8 min, ferment at 28°C in an aerobic environment for 24 h. After fermentation, take out half of the fermented material and place it in another conical flask. Add 90 mL of 70% ethanol solution to each of the two conical flasks containing the fermented material, shake well, and place them in a constant temperature water bath at 80°C for reflux extraction for 180 min. Shake the conical flask every 30 min during this period. Finally, filter the materials in the flask by suction, and the combined filtrate is the crude extract of puerarin; in this step, Aspergillus niger ferments Pueraria lobata under the irradiation of an ultraviolet lamp, and the puerarin extract obtained after fermentation can accelerate alcohol metabolism and protect the liver, and the puerarin content extracted after fermenting for 24 h after irradiating with an ultraviolet lamp for 8 min is the highest.

[0031] S2. Add anhydrous ethanol to the scutellaria powder to immerse the scutellaria powder, soak and stir for 12 hours, repeat the operation twice, and dry at 40°C to obtain defatted scutellaria powder; weigh the dried defatted scutellaria powder, add ultrapure water at a solid-liquid ratio of 1:20 (g / mL), extract in a boiling water bath for 2 hours, filter, discard the filtrate, collect the residue and repeat the extraction twice; add 0.5 mol / L NaOH solution to the filter residue at a solid-liquid ratio of 1:30 (g / mL), let it stand in a 4°C refrigerator for 12 hours, centrifuge at 6000 r / min for 20 minutes, collect the supernatant, add glacial acetic acid to the supernatant, adjust the pH to 7, centrifuge and collect the colloidal precipitate, wash the precipitate with ultrapure water, and vacuum freeze-dry to obtain insoluble scutellaria polysaccharide; the insoluble scutellaria polysaccharide extracted in this step shortens the sobering time and helps to reduce alcohol-induced liver cell damage.

[0032] S3, Lactobacillus plantarum CICC22697 was inoculated into MRS solid medium for slant activation, the culture temperature was 37°C, and the activation was carried out at constant temperature for 24 h. The activated strains were inoculated into LB medium again and shaken under the same conditions until the middle and late logarithmic growth phase of the strains to obtain a concentration of 10 8 CFU / mL of Lactobacillus plantarum CICC22697 bacterial liquid was set aside; 100 parts of each of the Chinese medicinal materials, namely, oriental rhizome, stir-fried atractylodes macrocephala, talc, lotus leaf, kudzu flower, ebony plum and tremella, were weighed by weight, and ground into 0.4 mm powder particles respectively, mixed, added with 8 times the weight of water, and boiled for 1 hour, filtered, the filter residue was added with 5 times the weight of water, boiled for 1 hour, filtered, the two filtrates were combined and heated and boiled to 1 / 2 of the volume, and recorded as 100 parts by weight. Then, 1 part of coptis root, 1 part of codonopsis pilosula, 1 part of pinellia tuber, 0.1 part of liquorice, 0.1 part of scorched shenqu, 0.5 part of scorched malt, 0.5 part of hovenia dulcis, 0.1 part of dried ginger, 0.1 part of green peel, 0.1 part of dried tangerine peel, 0.1 part of angelica sinensis, 0.1 part of melted yellow gelatin (purchased from Shandong Dong'e Ejiao Co., Ltd.), 0.1 part of donkey-hide gelatin (purchased from Shandong Dong'e Ejiao Co., Ltd.) and 0.1 part of isomaltooligosaccharide (purchased from Chengdu Wanxiang Hongrun Biotechnology Co., Ltd.) were added to the above-mentioned medicinal solution boiled to 1 / 2 volume, and 30 parts of water were added and steamed for 3 hours, and then 8 parts of 10% concentration of sesame seed were added. 8CFU / mL Lactobacillus plantarum CICC 22697 bacterial liquid, and ferment for 4 days under aerobic conditions at a temperature of 37°C. Filter the obtained fermentation broth to remove the supernatant. Add 30 parts of the crude puerarin extract prepared in step S1 and 3 parts of the insoluble baicalin polysaccharide prepared in step S2 to 90 parts of the supernatant, mix evenly, heat at 65°C for 10 h, and then transfer it to a container and slowly simmer over low heat for 10 h until it becomes solid, which is the traditional Chinese medicine composition for relieving alcohol, protecting the liver and preventing hangover, also called Jiu Hu Gao. The traditional Chinese medicine obtained when the weight ratio of the crude puerarin extract prepared in step S1 to the insoluble baicalin polysaccharide prepared in step S2 is 10:1 has the ability to significantly reduce the degree of hepatocyte lesions.

[0033] Comparative Example 1: Except that in step S1, it is irradiated with ultraviolet light for 2 min, the rest are the same as in Example 1.

[0034] Comparative Example 2: Except that in step S1, it is irradiated with ultraviolet light for 4 min, the rest are the same as in Example 1.

[0035] Comparative Example 3: Except that in step S1, it is fermented under ultraviolet light irradiation for 6 min, the rest are the same as in Example 1.

[0036] Comparative Example 4: Except that in step S1, it is fermented under ultraviolet light irradiation for 10 min, the rest are the same as in Example 1.

[0037] Comparative Example 5: Except that in step S1, it is fermented under ultraviolet light irradiation for 12 min, the rest are the same as in Example 1.

[0038] Comparative Example 6: Except that in step S1, the fermentation under ultraviolet light irradiation is changed to direct fermentation without prior ultraviolet light irradiation, the rest are the same as in Example 1.

[0039] Figure 1 This is the influence of the fermentation duration under ultraviolet light irradiation in Example 1 of the present invention and Comparative Examples 1 - 6 on the extraction of puerarin. Under the condition that other conditions remain unchanged, the content of puerarin is measured under the conditions of ultraviolet light irradiation for 2 min, 4 min, 6 min, 8 min, 10 min, 12 min and 0 min respectively. The results are shown in Figure 1 , as can be seen from Figure 1 that with the increase of ultraviolet light irradiation, the extracted puerarin content will gradually increase and then decrease. When the irradiation time in Example 1 of the present invention is 8 min, the content is the highest. When the fermentation time is greater than 8 min, the extraction amount of puerarin decreases. However, when directly fermenting without ultraviolet light irradiation, the obtained puerarin content decreases significantly, only 5 mg / L. Therefore, only when using ultraviolet light irradiation for 8 min and then fermenting for 24 h after irradiation can the best puerarin content be obtained.

[0040] Example 2

[0041] S1. Streak inoculate Aspergillus niger CICC2160 on a PDA slant. After culturing at 28 °C for 70 h, the slant is covered with black spores. Under aseptic conditions, pick 1 loop of spores and inoculate them into 10 mL of sterilized 0.85% physiological saline containing 0.1% Tween-80, and shake well to obtain an Aspergillus niger spore suspension with a concentration of 3×10 7 CFU / mL; Weigh 9.2 g of Pueraria lobata residue with a particle size of 0.42 mm and 5.2 g of wheat bran, add 11 mL of distilled water to obtain the initial solid fermentation medium. Then, sterilize the medium in an autoclave at 122 °C for 32 min and set aside. Take 11 g of the prepared solid fermentation medium in a conical flask. After sterilization, inoculate 1 mL of the Aspergillus niger spore suspension, shake the conical flask to make the spore suspension evenly distributed in the medium as much as possible. After irradiating with an ultraviolet lamp (K = 266 nm, 40 W) for 8 min, ferment at 28 °C in an aerobic environment for 24 h. After fermentation, take out half of the fermented material and place it in another conical flask. Add 92 mL of 70% ethanol solution to each of the two conical flasks containing the fermented material, shake well, and place them in a constant temperature water bath at 80 °C for reflux extraction for 185 min. During this period, shake the conical flask every 30 min. Finally, filter the material in the flask, and the combined filtrate is the crude extract of puerarin; in this step, Aspergillus niger ferments Pueraria lobata under the irradiation of an ultraviolet lamp, and the puerarin extract obtained after fermentation can accelerate alcohol metabolism and protect the liver, and the puerarin content extracted after 24 h of fermentation after 8 min of ultraviolet lamp irradiation is the highest.

[0042] S2. Add anhydrous ethanol to the Scutellaria baicalensis powder to submerge the powder, soak and stir for 13 h, repeat the operation 3 times, and dry at 45 °C to obtain defatted Scutellaria baicalensis powder; Weigh the dried defatted Scutellaria baicalensis powder, add ultrapure water according to the solid-liquid ratio of 1:20 (g / mL), extract in a boiling water bath for 3 h, filter, discard the filtrate, and collect the residue and repeat the extraction 2 times; Add 0.5 mol / L NaOH solution to the filter residue according to the solid-liquid ratio of 1:30 (g / mL), let it stand in a 4 °C refrigerator for 13 h, centrifuge at 6500 r / min for 23 min, collect the supernatant, add glacial acetic acid to the supernatant to adjust the pH value to 7, centrifuge and collect the colloidal precipitate, wash the precipitate with ultrapure water, and vacuum freeze-dry to obtain insoluble Scutellaria baicalensis polysaccharide; the insoluble Scutellaria baicalensis polysaccharide extracted in this step shortens the hangover time and helps to reduce alcohol-induced liver cell damage.

[0043] S3. Inoculate Lactobacillus plantarum CICC22697 on an MRS solid medium for slant activation. The culture temperature is 37 °C, and incubate at a constant temperature for 24 h. Transfer the activated strain to an LB medium and perform shaking culture under the same conditions until the middle and late logarithmic growth phase of the strain is obtained, and a concentration of 10 8CFU / mL of lactobacillus liquid was set aside; 105 parts each of the Chinese medicinal materials Alisma orientalis, stir-fried Atractylodes macrocephala, talc, lotus leaf, kudzu flower, black plum and Tremella fuciformis were weighed by weight, and ground into 0.3 mm powder particles respectively, mixed, added with 9 times the weight of water, boiled for 1.5 hours, filtered, added 5 times the weight of water to the residue, boiled for 1.5 hours, filtered, combined the two filtrates and heated and boiled to 1 / 2 of the volume, recorded as 100 parts by weight. Then, 2 parts of coptis root, 2 parts of coptis chinensis, 2 parts of pinellia tuber, 0.5 parts of licorice, 0.5 parts of scorched shenqu, 1 part of scorched malt, 1 part of huoyizi, 0.5 parts of dried ginger, 0.5 parts of green peel, 0.5 parts of dried tangerine peel, 0.5 parts of angelica, 0.5 parts of melted yellow gelatin (purchased from Shandong Dong'e Ejiao Co., Ltd.), 0.5 parts of donkey-hide gelatin (purchased from Shandong Dong'e Ejiao Co., Ltd.) and 0.5 parts of isomaltooligosaccharide (purchased from Chengdu Wanxiang Hongrun Biotechnology Co., Ltd.) were added to the above-mentioned medicinal solution boiled to 1 / 2 volume, and 31 parts of water were added and steamed for 3 hours, and then 9 parts of 10% concentration were added. 8 CFU / mL of plant lactobacillus CICC22697 bacterial liquid, and fermented it under aerobic conditions at a temperature of 37°C for 5 days, the obtained fermented liquid was filtered to obtain the supernatant, 32 parts of the puerarin crude extract prepared in step S1 and 3.2 parts of the insoluble scutellaria polysaccharide prepared in step S2 were added to 90 parts of the supernatant, and then mixed evenly and heated at 75°C for 10 hours, and then transferred to a container and slowly simmered over low heat for 11 hours to a solid state, which is a Chinese medicine composition for sobering up, protecting the liver and preventing hangovers, also called Jiuhu Gao. In this step, the Chinese medicinal material obtained when the weight ratio of the puerarin crude extract prepared in step S1 to the insoluble scutellaria polysaccharide prepared in step S2 is 10:1 has the ability to significantly reduce the degree of liver cell lesions.

[0044] Comparative Example 7: Except that the fermentation under ultraviolet light in step S1 is changed to direct fermentation without first using ultraviolet light, the rest is the same as Example 2.

[0045] Comparative Example 8: Except that the puerarin crude extract in step S3 is replaced by pueraria root residue, the rest is the same as Example 2.

[0046] Comparative Example 9: Except that the insoluble Scutellaria baicalensis polysaccharide in step S3 is replaced by Scutellaria baicalensis, the rest is the same as Example 2.

[0047] Comparative Example 10: Except that after boiling for 3 h in step S3, Lactobacillus plantarum CICC22697 was added for fermentation for 5 days, it was replaced by boiling for 3 h, filtering directly without fermentation, retaining 90 parts of the supernatant, adding 32 parts of the crude puerarin extract prepared in step S1 and 3.2 parts of the insoluble Scutellaria baicalensis polysaccharide prepared in step S2, mixing evenly, and heating at 75°C for 10 h, the rest was the same as Example 2.

[0048] Comparative Example 11: Except that Aspergillus niger CICC2160 in step S1 was replaced with Aspergillus niger CICC40554, the rest was the same as in Example 2. Aspergillus niger CICC40554 was purchased from Beijing Yuwei Technology Co., Ltd.

[0049] Comparative Example 12: Except that Aspergillus niger CICC2160 in step S1 was replaced with Aspergillus niger ATCC10864, the rest was the same as in Example 2. Aspergillus niger ATCC10864 was purchased from Shandong Top Biological Engineering Co., Ltd.

[0050] Determination of hangover and hangover time:

[0051] 170 male KM mice (purchased from Shanghai Model Organisms Center, Inc.) with a body weight of 23 - 25 g were gavaged with 56-degree Red Star Erguotou at a volume of 14 mL / kg·bw. Mice with a drunkenness time greater than 6 h or less than 10 min were excluded (a total of 10 mice were excluded). If a mouse maintained a dorsal-down posture for more than 30 s, it was considered that the righting reflex disappeared, indicating drunkenness. The remaining 160 mice were randomly divided into a normal group, an Example 2 group, a Comparative Example 7 group, a Comparative Example 8 group, a Comparative Example 9 group, a Comparative Example 10 group, a Comparative Example 11 group, and a Comparative Example 12 group, a total of 8 groups, with 20 mice in each group. Each group was gavaged with alcohol at a volume of 14 mL / kg·bw, and the drunkenness and hangover times of the mice before drug administration were measured, as shown in Table 1 and Table 2. According to the results of preliminary pre-experiments, when continuously gavaged with alcohol at a volume of 14 mL / kg·bw for a long time, the mortality rate of mice was relatively high. To reduce the mortality rate of mice, the subsequent alcohol gavage volume was reduced to 12 mL / kg·bw. Each group of mice was gavaged with alcohol at a volume of 12 mL / kg·bw every day. 30 min after gavage, the normal group was given water at a volume of 10 mL / kg, and the Example 2 and Comparative Example 7 - 12 groups were respectively gavaged with a suspension prepared from each traditional Chinese medicine composition of the same volume plus distilled water. The dosage of each traditional Chinese medicine composition was 20 mg / kg. After continuous administration for 10 d, the mice were gavaged with alcohol at a volume of 14 mL / kg·bw again, and the drunkenness and hangover times of the mice after drug administration were observed and recorded, as shown in Table 1 and Table 2.

[0052] Table 1

[0053] Group Drunkenness time before administration / min Drunkenness time after administration / min Normal group 35.31±3.45 34.14±4.31 Example 2 33.56±7.21 64.37±2.31 Comparative Example 7 36.90±6.95 41.01±3.97 Comparative Example 8 37.61±5.26 54.23±4.36 Comparative Example 9 37.84±6.58 59.46±2.74 Comparative Example 10 36.97±6.76 42.33±4.58 Comparative Example 11 34.25±4.28 40.21±1.89 Comparative Example 12 35.46±1.32 42.13±2.65 Table 2

[0054] Group Sobering-up time before administration / min Sobering-up time after administration / min Normal group 139.64±9.54 134.75±6.32 Example 2 135.21±9.31 33.23±5.29 Comparative Example 7 130.09±12.20 92.31±6.78 Comparative Example 8 141.12±12.76 101.20±6.84 Comparative Example 9 145.13±10.70 98.74±5.35 Comparative Example 10 124.25±8.02 104.23±6.44 Comparative Example 11 140.65±7.65 99.36±8.99 Comparative Example 12 138.78±9.42 97.27±9.62 Table 1 shows the comparison of the drunkenness time of the normal group, Example 2, Comparative Example 7, Comparative Example 8, Comparative Example 9, Comparative Example 10, Comparative Example 11, and Comparative Example 12 in mice before and after administration. The sobering-up time of the normal group did not change after giving water. However, compared with before administration, the drunkenness time of the groups of Example 2, Comparative Example 7, Comparative Example 8, Comparative Example 9, Comparative Example 10, Comparative Example 11, and Comparative Example 12 after administration was prolonged by 91.80%, 11.13%, 44.19%, 57.14%, 14.50%, 17.40%, and 18.81% respectively, indicating that the traditional Chinese medicine in Example 2 of the present invention has an obvious anti-hangover effect.

[0055] Table 2 shows the comparison of the sobering-up time of the normal group, Example 2, Comparative Example 7, Comparative Example 8, Comparative Example 9, Comparative Example 10, Comparative Example 11, and Comparative Example 12 in mice before and after administration. When only water was given to the normal group, there was little difference in the drunkenness time. However, compared with before administration, the sobering-up time of the groups of Example 2, Comparative Example 7, Comparative Example 8, Comparative Example 9, Comparative Example 10, Comparative Example 11, and Comparative Example 12 after administration was shortened by 75.42%, 29.04%, 28.29%, 31.96%, 16.11%, 29.36%, and 29.91% respectively.

[0056] The results of Table 1 and Table 2 show that ultraviolet lamp irradiation for 8 min, crude puerarin extract, insoluble baicalin polysaccharide, traditional Chinese medicine fermentation, and the type of Aspergillus niger all have certain effects on anti-hangover and sobering-up. However, when they exist simultaneously, the traditional Chinese medicine prepared in Example 2 of the present invention has an obvious anti-hangover effect of slowing down the drunkenness process and can significantly shorten the sobering-up time. Therefore, the traditional Chinese medicine composition prepared by the present invention has the effects of relieving alcohol and anti-hangover.

[0057] Example 3

[0058] S1. Streak inoculate Aspergillus niger CICC2160 on a PDA slant and culture it at 28 °C for 70 h until the slant is covered with black spores. Under sterile conditions, pick 1 loop of spores and inoculate them into 10 mL of sterilized 0.85% physiological saline containing 0.1% Tween-80 and shake well to obtain a bacterial suspension with a concentration of 5×10 7Aspergillus niger spore suspension at CFU / mL; Weigh 9.8 g of Pueraria lobata residue with a particle size of 0.45 mm and 5.8 g of wheat bran, add 13 mL of distilled water to obtain the initial solid fermentation medium. Then, sterilize the medium in an autoclave at 128 °C for 38 min and set aside. Take 11 g of the prepared solid fermentation medium in a conical flask, and after sterilization, inoculate 1 mL of Aspergillus niger spore suspension. Oscillate the conical flask to make the spore suspension evenly distributed in the medium as much as possible. After irradiating with an ultraviolet lamp (K = 266 nm, 40 W) for 8 min, ferment at 28 °C in an aerobic environment for 24 h. After fermentation, take out half of the fermented material and place it in another conical flask. Add 98 mL of 70% ethanol solution to each of the two conical flasks with the fermented material, shake well, and place them in a constant temperature water bath at 80 °C for reflux extraction for 190 min. During this period, shake the conical flask every 30 min. Finally, filter the material in the flask, and the combined filtrate is the crude extract of puerarin; in this step, Aspergillus niger ferments Pueraria lobata under the irradiation of an ultraviolet lamp, and the puerarin extract obtained after fermentation can accelerate alcohol metabolism and protect the liver. And after irradiating with an ultraviolet lamp for 8 min, the puerarin content extracted after 24 h of fermentation is the highest.

[0059] S2. Add anhydrous ethanol to the Scutellaria baicalensis powder to submerge the powder, soak and stir for 13 h, repeat the operation 3 times, and dry at 50 °C to obtain defatted Scutellaria baicalensis powder; Weigh the dried defatted Scutellaria baicalensis powder, add ultrapure water according to the solid-liquid ratio of 1:20 (g / mL), extract in a boiling water bath for 4 h, filter, discard the filtrate, and collect the residue for extraction 2 times; Add 0.5 mol / L NaOH solution to the filter residue according to the solid-liquid ratio of 1:30 (g / mL), let it stand in a 4 °C refrigerator for 13 h, centrifuge at 7500 r / min for 28 min, collect the supernatant, add glacial acetic acid to the supernatant to adjust the pH value to 7, centrifuge and collect the colloidal precipitate, wash the precipitate with ultrapure water, and vacuum freeze-dry to obtain insoluble Scutellaria baicalensis polysaccharide; the insoluble Scutellaria baicalensis polysaccharide extracted in this step shortens the hangover time and helps to reduce the damage of alcohol-induced hepatocytes.

[0060] S3. Inoculate Lactobacillus plantarum CICC22697 on MRS solid medium for slant activation, the culture temperature is 37 °C, and incubate at a constant temperature for 24 h for activation. Then, transfer the activated strain to LB medium and perform shaking culture under the same conditions until the mid-late logarithmic growth phase of the strain to obtain a concentration of 10 8Lactobacillus bacterial liquid at CFU / mL was prepared for later use. Weigh 110 parts of traditional Chinese medicinal materials Alisma orientale, stir-fried Atractylodes macrocephala, Talc, Lotus leaf, Flos Puerariae, Fructus Mume, and Tremella fuciformis by weight respectively, and grind them into powdery particles with a size of 0.1 mm. After mixing, add 9 times the weight of water and boil for 1.5 h, then filter. Add 5 times the weight of water to the filter residue and boil for 1.5 h, then filter. Combine the two filtrates and heat and boil until the volume is reduced to 1 / 2, which is recorded as 100 parts by weight. Then, add 3 parts of Coptis chinensis, 3 parts of Codonopsis pilosula, 3 parts of Pinellia ternata processed with ginger, 2 parts of Glycyrrhiza uralensis, 2 parts of stir-fried Medicago sativa, 1.5 parts of stir-fried Hordeum vulgare, 1.5 parts of Hovenia dulcis, 1.5 parts of dried ginger, 1.5 parts of Pericarpium Citri Reticulatae Viride, 1.5 parts of Pericarpium Citri Reticulatae, 1.5 parts of Angelica sinensis, 1.5 parts of melted Yellowish gelatin (purchased from Shandong Dong'e E-Jiao Co., Ltd.), 1.5 parts of E-Jiao (purchased from Shandong Dong'e E-Jiao Co., Ltd.), and 1.5 parts of isomaltooligosaccharide (purchased from Chengdu Wanxiang Hongrun Biotechnology Co., Ltd.) to the above-mentioned decoction with a volume reduced to 1 / 2 by weight, and add 34 parts of water and cook for 4 h. Then, inoculate 9 parts of Lactobacillus plantarum CICC22697 bacterial liquid with a concentration of 10 8 CFU / mL, and ferment for 6 days under aerobic conditions at a temperature of 37 °C. Filter the obtained fermentation broth to obtain the supernatant. Add 38 parts of the crude puerarin extract prepared in step S1 and 3.8 parts of the insoluble Scutellaria baicalensis polysaccharide prepared in step S2 to 90 parts of the supernatant, mix well, heat at 70 °C for 10 h, and then transfer to a container and slowly simmer over low heat for 11 h until it becomes solid, which is the traditional Chinese medicine composition for relieving alcohol, protecting the liver, and preventing hangover, also known as Jiu Hu Gao. In this step, the traditional Chinese medicine materials obtained when the weight ratio of the crude puerarin extract prepared in step S1 to the insoluble Scutellaria baicalensis polysaccharide prepared in step S2 is 10:1 have the ability to significantly reduce the degree of hepatocyte lesions.

[0061] Comparative Example 13: Except that the fermentation under ultraviolet light irradiation in step S1 was changed to direct fermentation without using ultraviolet light, the rest was the same as in Example 3.

[0062] Comparative Example 14: Except that the crude puerarin extract in step S3 was replaced with puerarin residue, the rest was the same as in Example 3.

[0063] Comparative Example 15: Except that the insoluble Scutellaria baicalensis polysaccharide in step S3 was replaced with Scutellaria baicalensis, the rest was the same as in Example 3.

[0064] Comparative Example 16: Except that after cooking for 4 h in step S3, adding Lactobacillus plantarum CICC22697 and fermenting for 6 days was replaced with directly filtering after cooking for 4 h without fermentation, leaving 90 parts of the supernatant, adding 38 parts of the crude puerarin extract prepared in step S1 and 3.8 parts of the insoluble Scutellaria baicalensis polysaccharide prepared in step S2, mixing well, and heating at 70 °C for 10 h, the rest was the same as in Example 3.

[0065] Comparative Example 17: Except that Aspergillus niger CICC 2160 in step S1 was replaced with Aspergillus niger CICC 40554, the rest was the same as in Example 3.

[0066] Comparative Example 18: Except that Aspergillus niger CICC 2160 in step S1 was replaced with Aspergillus niger ATCC 10864, the rest was the same as in Example 3.

[0067] Determination of liver function:

[0068] 170 male KM mice (purchased from Shanghai Model Organisms Center, Inc.) with a body weight of 23 - 25 g were gavaged with 56-degree Hongxing Erguotou at a volume of 14 mL / kg·bw. Mice with a drunkenness time greater than 6 h or less than 10 min were excluded (a total of 10 mice were excluded). If a mouse maintained a dorsal-down posture for more than 30 s, it was considered that the righting reflex disappeared, indicating drunkenness. The remaining 160 mice were randomly divided into a normal group, an Example 3 group, a Comparative Example 13 group, a Comparative Example 14 group, a Comparative Example 15 group, a Comparative Example 16 group, a Comparative Example 17 group, and a Comparative Example 18 group, a total of 8 groups, with 20 mice in each group. Each group was gavaged with alcohol at a volume of 14 mL / kg·bw. 30 min after gavage, the normal group was given water at a volume of 10 mL / kg, and the Example 3 group and the Comparative Example 13 - 18 groups were respectively given a suspension prepared by adding each traditional Chinese medicine composition and distilled water at the same volume. The dosage of each traditional Chinese medicine composition was 20 mg / kg. After fasting for 12 h, blood was collected by eye enucleation, centrifuged at 4000 r / min for 10 min, and the serum was separated. The levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the serum were detected using an automatic biochemical analyzer. The results are shown in Table 3.

[0069] Table 3

[0070] Group ALT / (IU / L) AST / (IU / L) Normal group 61.25±5.26 133.79±6.88 Example 3 38.47±1.87 94.58±4.70 Comparative Example 13 54.28±4.63 131.11±7.44 Comparative Example 14 49.88±2.46 131.82±7.96 Comparative Example 15 47.54±2.34 130.67±5.14 Comparative Example 16 57.29±3.61 132.69±2.65 Comparative Example 17 50.21±3.89 132.45±5.92 Comparative Example 18 49.81±5.23 133.09±6.47 ALT and AST are the most sensitive biochemical indicators directly reflecting hepatocyte damage. ALT is mainly distributed in the cytoplasm of hepatocytes, and even slight damage to hepatocytes can be reflected by ALT. Therefore, ALT is the most sensitive indicator of hepatocyte damage. Table 3 shows the liver function test results of the normal group, the Example 3 group, the Comparative Example 13 group, the Comparative Example 14 group, the Comparative Example 15 group, the Comparative Example 16 group, the Comparative Example 17 group, and the Comparative Example 18 group of mice after administration. As can be seen from the table, the serum ALT levels in the Example 3 group, the Comparative Example 13 - 18 groups decreased by 37.19%, 11.37%, 18.56%, 22.38%, 6.47%, 18.01%, and 18.68% respectively. Compared with Example 3, the effects of Comparative Example 13, Comparative Example 14, Comparative Example 15, Comparative Example 16, Comparative Example 17, and Comparative Example 18 in reducing alcohol-induced hepatocyte damage were poorer, indicating that the traditional Chinese medicine composition in the Example 3 group of the present invention can effectively reduce alcohol-induced hepatocyte damage.

[0071] When hepatocytes are severely damaged and necrotic, the serum AST level rises. Therefore, the serum AST level indicates severe hepatocyte damage. However, as hepatocytes repair and regenerate, the serum AST level decreases and gradually returns to normal. Compared with the normal group, there was almost no change in the serum AST levels of Comparative Examples 13 to 18, only a slight decrease occurred. However, the serum AST in Example 3 decreased by 29.31%, indicating that Example 3 of the present invention has a certain effect of repairing hepatocyte damage and promoting hepatocyte regeneration, while Comparative Examples 13 to 18 can hardly promote hepatocyte regeneration. Therefore, the traditional Chinese medicine prepared by the present invention not only has the effect of reducing alcohol-induced hepatocyte damage, but also can repair hepatocyte damage and promote hepatocyte regeneration.

[0072] Example 4

[0073] S1. Streak inoculate Aspergillus niger CICC2160 on a PDA slant and culture it at 28 °C for 75 h until the slant is covered with black spores. Under sterile conditions, pick 1 loop of spores and inoculate them into 10 mL of sterilized 0.85% physiological saline containing 0.1% Tween-80 and shake well to obtain an Aspergillus niger spore suspension with a concentration of 6×10 7 CFU / mL; Weigh 10 g of Pueraria lobata residue with a particle size of 0.5 mm and 6 g of wheat bran, add 14 mL of distilled water to obtain the initial solid fermentation medium. Then, sterilize the medium in an autoclave at 130 °C for 40 min and set aside. Take 12 g of the prepared solid fermentation medium in a conical flask, inoculate 1 mL of the Aspergillus niger spore suspension after sterilization, shake the conical flask to make the spore suspension evenly distributed in the medium as much as possible, and ferment at 24 °C for 24 h in an aerobic environment after irradiating with an ultraviolet lamp (K = 266 nm, 40 W) for 8 min. After the fermentation is completed, take out half of the fermented material and place it in another conical flask. Add 100 mL of 70% ethanol solution to each of the two conical flasks containing the fermented material, shake well, and place them in a constant temperature water bath at 80 °C for reflux extraction for 200 min. Shake the conical flask every 30 min during this period. Finally, filter the material in the flask, and the combined filtrate is the crude puerarin extract; in this step, Aspergillus niger ferments Pueraria lobata under the irradiation of an ultraviolet lamp, and the puerarin extract obtained after fermentation can accelerate alcohol metabolism and protect the liver, and the puerarin content extracted after fermenting for 24 h after irradiating with an ultraviolet lamp for 8 min is the highest.

[0074] S2. Add anhydrous ethanol to the Scutellaria baicalensis powder to submerge it, soak and stir for 14 h, repeat the operation 4 times, dry at 60 °C to obtain defatted Scutellaria baicalensis powder; weigh the dried defatted Scutellaria baicalensis powder, add ultrapure water according to the solid-liquid ratio of 1:20 (g / mL), extract in a boiling water bath for 5 h, filter, discard the filtrate, collect the residue and repeat the extraction 2 times; add 0.5 mol / L NaOH solution to the filter residue according to the solid-liquid ratio of 1:30 (g / mL), let it stand in a 4 °C refrigerator for 14 h, centrifuge at 8000 r / min for 30 min, collect the supernatant, add glacial acetic acid to the supernatant to adjust the pH value to 7, centrifuge and collect the colloidal precipitate, wash the precipitate with ultrapure water, and vacuum freeze-dry to obtain insoluble Scutellaria baicalensis polysaccharide; the insoluble Scutellaria baicalensis polysaccharide extracted in this step shortens the hangover time and helps to reduce the damage of alcohol-induced hepatocytes.

[0075] S3. Inoculate Lactobacillus plantarum CICC22697 on MRS solid medium for slant activation, the culture temperature is 37 °C, incubate at a constant temperature for 24 h for activation, transfer the activated strain to LB medium again, and perform shaking culture under the same conditions until the mid to late logarithmic growth phase of the strain to obtain a Lactobacillus plantarum bacterium solution with a concentration of 10 8 CFU / mL for standby; weigh 120 parts each of the Chinese medicinal materials Alisma orientale, stir-fried Atractylodes macrocephala, Talc, Lotus leaf, Flos Puerariae lobatae, Dark plum, and Tremella fuciformis by weight, and grind them into powdery particles with a size of 0.005 mm respectively. After mixing, add 10 times the weight of water and boil for 2 h, then filter. Add 5 times the weight of water to the filter residue and boil for 2 h, filter, combine the two filtrates and heat and boil to 1 / 2 of the volume, which is recorded as 100 parts by weight. Then add 3 parts of Coptis chinensis, 3 parts of Codonopsis pilosula, 3 parts of Pinellia ternata processed with ginger, 3 parts of Glycyrrhiza uralensis, 3 parts of stir-fried Medicago sativa, 2 parts of stir-fried Hordeum vulgare, 2 parts of Hovenia dulcis, 2 parts of dried ginger, 2 parts of Pericarpium Citri Reticulatae Viride, 2 parts of Pericarpium Citri Reticulatae, 2 parts of Angelica sinensis, 2 parts of melted and well-prepared yellow gelatin (purchased from Shandong Dong'e E-Jiao Co., Ltd.), 2 parts of donkey-hide gelatin (purchased from Shandong Dong'e E-Jiao Co., Ltd.), and 2 parts of isomaltooligosaccharide (purchased from Chengdu Wanxiang Hongrun Biotechnology Co., Ltd.) to the above-mentioned decoction boiled to 1 / 2 volume by weight, add 35 parts of water and steam for 4 h, then inoculate 10 parts of Lactobacillus plantarum CICC22697 bacterium solution with a concentration of 10 8 CFU / mL, and ferment for 7 days under aerobic conditions at 37 °C. After filtering the obtained fermentation broth, take the supernatant, add 40 parts of the crude puerarin extract prepared in step S1 and 4 parts of the insoluble Scutellaria baicalensis polysaccharide prepared in step S2 to 100 parts of the supernatant, mix evenly, heat at 75 °C for 10 h, and then transfer to a container and slowly simmer over low heat for 12 h until it becomes solid, which is the traditional Chinese medicine composition for relieving hangover, protecting the liver and preventing hangovers, also known as Jiuhu Gao. The traditional Chinese medicine materials obtained when the weight ratio of the crude puerarin extract prepared in step S1 to the insoluble Scutellaria baicalensis polysaccharide prepared in step S2 is 10:1 have the ability to significantly reduce the degree of hepatocyte lesions.

[0076] Comparative Example 19: Except adding 40 parts of crude puerarin extract and 3 parts of insoluble scutellaria baicalensis polysaccharide (13.3:1) by weight in step S4, the rest are the same as in Example 4.

[0077] Comparative Example 20: Except adding 40 parts of crude puerarin extract and 5 parts of insoluble scutellaria baicalensis polysaccharide (8:1) by weight in step S4, the rest are the same as in Example 4.

[0078] Comparative Example 21: Except adding 50 parts of crude puerarin extract and 4 parts of insoluble scutellaria baicalensis polysaccharide (12.5:1) by weight in step S4, the rest are the same as in Example 4.

[0079] Comparative Example 22: Except adding 30 parts of crude puerarin extract and 4 parts of insoluble scutellaria baicalensis polysaccharide (7.5:1) by weight in step S4, the rest are the same as in Example 4.

[0080] Test for liver cases:

[0081] 170 male KM mice (purchased from Shanghai Model Organisms Center, Inc.) with a body weight of 23 - 25 g. These 170 mice were intragastrically administered 56-degree Hongxing Erguotou at a volume of 14 mL / kg·bw. Mice with a drunken time greater than 6 h or less than 10 min were excluded (a total of 10 mice were excluded). If a mouse maintained a dorsal-down posture for more than 30 s, it was considered that the righting reflex disappeared, indicating drunkenness. The remaining 160 mice were randomly divided into Example 4 group, Comparative Example 19 group, Comparative Example 20 group, Comparative Example 21 group, and Comparative Example 22 group, a total of 5 groups, with 32 mice in each group. Each group was intragastrically administered alcohol at a volume of 14 mL / kg·bw. 30 min after alcohol administration, the normal group was intragastrically administered water at a volume of 10 mL / kg, and the Example 4 and Comparative Example 19 - 22 groups were respectively intragastrically administered a suspension made of each traditional Chinese medicine composition and distilled water with the same volume. The dosage of each traditional Chinese medicine composition was 20 mg / kg. After fasting for 12 h, blood was collected by eye enucleation, centrifuged at 4000 r / min for 10 min to separate serum. After collecting the serum, the mice were sacrificed by cervical dislocation, the livers were removed, and liver tissues with a size of about 0.5×0.5 cm were taken, fixed with 10% formalin, and embedded in paraffin. After sectioning, routine HE staining was performed to observe the pathological changes of mouse liver tissues.

[0082] Figures 2 - 6 They are respectively the pathological results diagrams of the liver tissues of the mice in Example 4 and Comparative Examples 19 - 22 of the present invention. As Figure 2 shown, under light microscopy, it shows that the hepatic cell cords in the normal group are radial, arranged regularly and evenly, and the hepatocytes have no edema, degeneration, or necrosis; as Figures 3 - 6As shown, stem cell lesions occurred in the mice of Comparative Examples 19 to 22, specifically manifested as obvious hepatocyte swelling, rupture, large areas of necrosis, nuclear condensation, and vacuolar degeneration of different sizes in the cells. The above phenomena indicate that Step S3 of the traditional Chinese medicine prepared in Example 4 of the present invention is extremely crucial. The Chinese medicinal material Jiuhu Gao obtained when the weight ratio of puerarin crude extract to insoluble baicalin polysaccharide in this step is only 10:1 has the ability to significantly reduce the degree of hepatocyte lesions and is also helpful for reducing alcohol-induced hepatocyte damage.

[0083] The above-described embodiments merely describe the preparation process of the present invention and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, various deformations and improvements made by those of ordinary skill in the art to the technical solutions of the present invention shall fall within the protection scope determined by the claims of the present invention.

Claims

1. A method for preparing a Chinese medicine composition for sobering up, protecting the liver and preventing hangover, characterized in that: The specific preparation steps are as follows: S1. Inoculate Aspergillus niger CICC2160 on a PDA slant and culture at 28°C for 70 h. Under sterile conditions, take 1 ring of spores and inoculate them into 10 mL of sterilized 0.85% saline containing 0.1% Tween-80 and shake well to obtain a concentration of 3×10 7 CFU / mL of Aspergillus niger suspension; weigh 9.2g of kudzu root residue with a particle size of 0.4-0.5 mm and 5.2g of wheat bran, and add 11mL of distilled water to obtain a solid fermentation medium, sterilize at 120-130℃ for 30-40min, take 11g of the prepared solid fermentation medium and inoculate 1mL of Aspergillus niger suspension, shake to make the bacterial suspension evenly distributed, irradiate with ultraviolet light for 8min, and ferment at 28℃ for 24h in an aerobic environment. After the fermentation is completed, divide the fermentation into two fermentation materials, add 92mL of 70% ethanol solution to each of the two fermentation materials, shake well, and place in a constant temperature water bath at 80℃ for reflux extraction for 180-200min, shake once every 30min, and finally filter the two materials, and combine the filtrates to obtain a crude puerarin extract; S2. Add anhydrous ethanol to the scutellaria powder, immerse and stir for 12-14 h, repeat the operation 2-4 times, and dry at 40-60°C to obtain defatted scutellaria powder; use ultrapure water at a solid-liquid ratio of 1:20 (g / mL) to extract the defatted scutellaria powder in a boiling water bath for 2-5 h, filter, discard the filtrate, and repeat the extraction twice; add 0.5 mol / L NaOH solution to the filter residue at a solid-liquid ratio of 1:30 (g / mL), let it stand in a refrigerator at 4°C for 12-14 h, centrifuge at 6000-8000 r / min for 20-30 min, collect the supernatant, adjust the pH to 7, centrifuge, collect the colloidal precipitate, wash and freeze-dry to obtain insoluble scutellaria polysaccharide; S3, plant lactobacillus CICC22697 was inoculated into MRS solid culture medium and cultured at 37°C for 24 hours, and then inoculated into LB medium and cultured until the middle and late logarithmic growth phase to obtain a concentration of 10 8 CFU / mL of plant lactobacillus liquid; weigh 105 parts of oriental rhizome, stir-fried atractylodes macrocephala, talcum powder, lotus leaf, kudzu flower, black plum and white fungus respectively, and grind them into 0.4 mm~0.005 mm powder particles, add 9 times the weight of water after mixing and boil for 1.5h, filter, add 5 times the weight of water to the filter residue and boil for 1.5h, filter, combine the filtrate for 2 times and heat and boil to 1 / 2 volume, record it as 100 parts by weight, then add 2 parts of coptis root, 2 parts of codonopsis pilosula, 2 parts of ginger pinellia, 0.5 parts of licorice, 0.5 parts of scorched Shenqu, 1 part of scorched malt, 1 part of Hovenia dulcis, 0.5 parts of dried ginger, 0.5 parts of green peel, 0.5 parts of tangerine peel, 0.5 parts of angelica, 0.5 parts of melted yellow gelatin, 0.5 parts of donkey-hide gelatin and 0.5 parts of oligosaccharide, add 31 parts of water and steam for 3h, then access 9 parts of 10 8 CFU / mL of plant lactobacillus liquid is prepared, and fermented under aerobic conditions at a temperature of 37°C for 5 days. The obtained fermented liquid is filtered to obtain the supernatant, and 32 parts of the puerarin crude extract prepared in step S1 and 3.2 parts of the insoluble scutellaria polysaccharide prepared in step S2 are added to 90 parts of the supernatant, and then the mixture is evenly mixed and heated at 65-75°C for 10 h, and then transferred to a container and slowly simmered over low heat for 10-12 h until it is solid, thereby obtaining a traditional Chinese medicine composition for sobering up, protecting the liver and preventing hangover.

2. The method for preparing a Chinese medicine composition for sobering up, protecting the liver and preventing hangover according to claim 1, characterized in that: In the step S1, Pueraria root residue with a particle size of 0.4 mm is taken.

3. The method for preparing a Chinese medicine composition for sobering up, protecting the liver and preventing hangover according to claim 2, characterized in that: In the step S1, the culture medium is sterilized in an autoclave at 120° C. for 30 min.

4. A Chinese medicine composition for sobering up, protecting the liver and preventing hangover according to claim 3, characterized in that: In the step S2, anhydrous ethanol is added to the scutellaria powder to immerse the scutellaria powder, and the immersion and stirring are performed for 12 hours, and the operation is repeated twice, and the powder is dried at 40°C.

5. The method for preparing a Chinese medicine composition for sobering up, protecting the liver and preventing hangover according to claim 4, characterized in that: In the step S2, centrifugation is performed at a speed of 6000 r / min for 20 min.

6. The method for preparing a Chinese medicine composition for sobering up, protecting the liver and preventing hangover according to claim 5, characterized in that: The particle size in step S3 is 0.4 mm.

7. A Chinese medicinal composition for sobering up, protecting the liver and preventing hangovers obtained by the method for preparing the Chinese medicinal composition for sobering up, protecting the liver and preventing hangovers according to any one of claims 1 to 6.

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