Gel with skin improvement and whitening efficacy and preparation method thereof

Abstract

The present application relates to a kind of gel with skin improvement and whitening effect and its preparation method, belong to cosmetic composition and its preparation method technical field.The gel with skin improvement and whitening effect in the present application, at least including: recombinant humanized Ⅲ type collagen 0.1%~0.5%, budding short stalk enzyme polysaccharide 0.2%~1%, nato glue 0.2%~0.6%, carbomer 0.1%~0.5%, triethanolamine 0.1%~0.5%, 1,2-pentanediol 1%~4%, glycerol 0.1%~3%, phenoxyethanol 0.1%~3%, the rest is water by mass percentage.The present application can not only improve skin by reasonable collocation the proportion of three components of recombinant humanized Ⅲ type collagen, nato glue and budding short stalk enzyme polysaccharide, but also reach the effect of whitening by inhibiting the activity of tyrosinase, realize the synergistic effect.

Description

TECHNICAL FIELD

[0001] The present application relates to a gel with skin improvement and whitening effect and a preparation method thereof, and belongs to the technical field of cosmetic compositions and preparation methods thereof. BACKGROUND

[0002] Collagen exists in the dermis of human beings, accounting for about 70% of the composition of skin tissue. Collagen is also known as "skin soft gold", which can moisturize the skin, supplement the required amino acids of the skin, and repair damaged skin. Adding collagen to cosmetics can provide essential nutrients for the skin, and the large number of hydrophilic groups contained therein have good moisturizing effect, and can prevent tyrosine in the skin from being converted into melanin, thereby having a whitening effect. In addition, collagen can also stabilize foam, regulate and stabilize pH, and reduce the irritation of surfactants, acids, bases and other substances to the skin and hair. Cosmetics containing recombinant collagen mainly include eye masks, facial masks, sprays, stock solutions, and freeze-dried powders. Studies have shown that applying skin care products containing collagen on the face can accelerate the synthesis of internal collagen in the skin, increase its activity, slow down the aging cycle of the skin, improve the tissue structure of the dermis and epidermis, and effectively alleviate fine lines and dryness on the face, making the skin more delicate and fair.

[0003] Skin care and beauty can help us protect the skin from pollutants and harmful substances in the daily environment, maintain the moisture of the skin, improve the elasticity and firmness of the skin, reduce the formation of fine lines and wrinkles, maintain the uniformity of skin pigmentation and reduce blemishes, and enhance the brightness and luster of the skin color. At present, the components of gel products for skin care are complex, most of which contain various preservatives, synthetic fragrances and other components, which may cause adverse reactions such as skin allergy and redness.

[0004] Most of the compositions on the market have single effect, which is difficult to achieve the expected effect. The present application aims to develop a composition with skin improvement and whitening effect. The components of the composition are reasonably matched to achieve synergistic effect. SUMMARY

[0005] In view of the deficiencies of the prior art, the present application provides a gel with skin improvement and whitening effect and a preparation method thereof. The gel comprises recombinant humanized type III collagen, short-spore polysaccharide and natto gum. Through methods such as adhesive tape stripping test, lactic acid stinging test, tyrosinase activity inhibition experiment and human open use spot-removing and whitening efficacy test, it is confirmed that the gel has good skin improvement and whitening effect.

[0006] The technical solution of the present application is as follows:

[0007] A composition with skin improvement and whitening effect, comprising, in mass percentage, 0.1-0.5% of recombinant humanized type III collagen, 0.2-1% of pullulan and 0.2-0.6% of Natrosol, and the balance being water.

[0008] According to the application, the amino acid sequence of the recombinant humanized type III collagen is shown in SEQ ID NO. 1.

[0009] A gel with skin improvement and whitening effect, comprising, in mass percentage, at least the above-mentioned composition with skin improvement and whitening effect.

[0010] According to the application, the gel with skin improvement and whitening effect further comprises, in mass percentage, 0.1-0.5% of carbomer.

[0011] According to the application, the gel with skin improvement and whitening effect further comprises, in mass percentage, 0.1-0.5% of triethanolamine.

[0012] According to the application, the gel with skin improvement and whitening effect further comprises, in mass percentage, 1-4% of 1,2-pentanediol.

[0013] According to the application, the gel with skin improvement and whitening effect further comprises, in mass percentage, 0.1-3% of glycerol.

[0014] According to the application, the gel with skin improvement and whitening effect further comprises, in mass percentage, 0.1-3% of phenoxyethanol.

[0015] Pullulan is a natural polysaccharide extracted from budding yeast. Budding yeast grows in the fermentation process of wine or beer, and its cell contains rich polysaccharide components. Pullulan has a large molecular weight and can form a moisturizing film on the skin surface to lock in skin moisture, thereby having good moisturizing effect.

[0016] Natrosol is a water-soluble polymer compound mainly made of ethenylated wood powder (usually cellulose) through oxyethylene reaction. In skin care products, Natrosol is often used as a thickening agent, moisturizing agent, emulsifying agent and other additives, which can improve the product texture, provide sufficient and long-lasting moisturizing effect for the skin, and has good skin permeability and skin texture improvement function. As a polysaccharide polymer, Natrosol can form a hydrogel and provide the skin with the required moisture, prevent water evaporation, and keep the skin moist. Studies have shown that products containing Natrosol have good moisturizing effect on dry skin.

[0017] The preparation method of the above-mentioned gel with skin improvement and whitening effects comprises the following steps:

[0018] (1) Take part of the prescription amount of purified water, slowly add the prescription amount of carbomer, glycerol and 1,2-pentanediol under stirring conditions, and dissolve at 40-60 DEG C under stirring;

[0019] (2) Add the prescription amount of triethanolamine to the solution of step (1), dissolve and stir, and wait for use;

[0020] (3) Add the prescription amount of natogel and pullulan to the remaining prescription amount of purified water, dissolve at 40-60 DEG C under stirring, and then add the prescription amount of recombinant humanized type III collagen, and continue stirring;

[0021] (4) Add the solution of step (3) to the solution of step (2), dissolve at 40-60 DEG C under stirring, add the prescription amount of phenoxyethanol, dissolve and stir, and prepare the gel.

[0022] According to the application, preferably, the part of the prescription amount of purified water in step (1) is 60-80% of the prescription amount of purified water.

[0023] According to the application, preferably, the time for stirring and dissolving in step (1) is 5-7 h.

[0024] According to the application, preferably, the time for stirring and dissolving in step (2) is 0.5-1 h.

[0025] According to the application, preferably, the time for stirring and dissolving in step (3) is 1-3 h.

[0026] According to the application, preferably, the time for continuing stirring in step (3) is 0.5-1 h.

[0027] According to the application, preferably, the time for stirring and dissolving at 40-60 DEG C in step (4) is 1-3 h.

[0028] According to the application, preferably, the time for stirring and dissolving after adding the prescription amount of phenoxyethanol in step (4) is 0.5-1.5 h.

[0029] The application accidentally found in experiments that the recombinant humanized type III collagen is added before triethanolamine, the gel becomes turbid, and the recombinant humanized type III collagen is added after triethanolamine, the gel is colorless and transparent, and the properties meet the expectations.

[0030] Beneficial effects:

[0031] 1、The present application can improve the skin, inhibit the activity of tyrosinase, achieve the effect of whitening and realize the synergistic effect by reasonably matching the proportion of three components of recombinant humanized type III collagen, natrosol and pullulan.

[0032] 2、The formula of the gel is simple, the natrosol in the gel can not only be used as an effective component, but also has a certain thickening effect, which can reduce the amount of thickening agent and avoid the side effects of too many components.

[0033] 3、The present application adjusts the adding sequence of recombinant humanized type III collagen, so that the structure of recombinant humanized type III collagen is not damaged, and the gel system is more stable.

[0034] 4、The raw material of recombinant humanized type III collagen is expensive, and the composition composed of recombinant humanized type III collagen, natrosol and pullulan reduces the proportion of recombinant humanized type III collagen under the condition that the total amount is basically unchanged, and the test results of skin improvement and whitening effect are similar. It is shown that the amount of natrosol and pullulan can be increased, and the amount of recombinant humanized type III collagen can be reduced to achieve similar effect, thereby saving the cost of raw materials. BRIEF DESCRIPTION OF DRAWINGS

[0035] Figure 1 The picture of the gel prepared for example 2 and comparative examples 7 and 8;

[0036] Figure 2 The difference value result of TEWL value after using the gel sample for 0.5h;

[0037] Figure 3 The difference value result of TEWL value after using the gel sample for 1.5h;

[0038] Figure 4 The difference value result of TEWL value after using the gel sample for 24h;

[0039] Figure 5 The lactic acid stinging score result after using the gel sample for 2.5min;

[0040] Figure 6 The lactic acid stinging score result after using the gel sample for 5min;

[0041] Figure 7 The change result of melanin content after using the gel sample for 2 weeks;

[0042] Figure 8 The change result of melanin content after using the gel sample for 4 weeks;

[0043] Figure 9 The change result of melanin content after using the gel sample for 8 weeks. Detailed Implementation

[0044] It should be noted that the following detailed descriptions are exemplary and intended to provide further explanation of the invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Unless otherwise specified, the raw materials involved in the embodiments are all commercially available products.

[0045] The amino acid sequence of recombinant humanized type III collagen is shown in SEQ ID NO.1 and is described in patent CN117886923A. It was prepared by high-density culture and purification as described in the examples.

[0046] Nystatin is available from Wuhan Huaxiang Kejie Biotechnology Co., Ltd., and is a premium grade product.

[0047] The budding short stalk enzyme polysaccharide can be obtained from Shandong Freda Biotechnology Co., Ltd.

[0048] The components and contents of the gels in the examples and comparative examples, in mass percentage, are shown in Table 1.

[0049] Table 1. Composition and content of gels in the examples and comparative examples

[0050]

[0051] Note: Comparative Example 5 replaces natto gum with an equal amount of guar gum.

[0052] Example 1

[0053] A gel with skin-improving and whitening effects, the raw material composition and content of which are shown in Table 1, and its preparation method includes the following steps:

[0054] (1) Take 80% of the prescribed amount of purified water, and slowly add the prescribed amount of carbomer, glycerol and 1,2-pentanediol under stirring conditions, and stir at 60°C for 5 hours to dissolve.

[0055] (2) Add the prescribed amount of triethanolamine to the solution from step (1), dissolve and stir for 1 hour, and set aside for use;

[0056] (3) Add the prescription amount of Natogum and budding short stem polysaccharide to the remaining 20% ​​of the purified water, stir and dissolve at 40°C for 3 hours, then add the prescription amount of recombinant humanized type III collagen and continue stirring for 0.5 hours.

[0057] (4) Add the solution from step (3) to the solution from step (2), stir and dissolve at 60°C for 1 hour, add the prescribed amount of phenoxyethanol and stir and dissolve for 1.5 hours to prepare a gel.

[0058] Example 2

[0059] A gel with skin improvement and whitening effect, the raw material composition and content are shown in Table 1, its preparation method comprises the following steps:

[0060] (1) 60% of the prescription amount of purified water is taken, and the prescription amount of carbomer, glycerol and 1,2-pentanediol is slowly added under stirring condition, and dissolved at 40℃ for 7h;

[0061] (2) The prescription amount of triethanolamine is added to the solution of step (1), and dissolved and stirred for 0.7h, and used;

[0062] (3) The prescription amount of natogel and pullulan is added to the remaining 40% of the prescription amount of purified water, and dissolved at 60℃ for 2h, and then the prescription amount of recombinant humanized type III collagen is added, and stirred for 0.7h;

[0063] (4) The solution of step (3) is added to the solution of step (2), and dissolved at 40℃ for 3h, and then the prescription amount of phenoxyethanol is added and dissolved and stirred for 0.5h, to prepare the gel.

[0064] Example 3

[0065] A gel with skin improvement and whitening effect, the raw material composition and content are shown in Table 1, its preparation method comprises the following steps:

[0066] (1) 60% of the prescription amount of purified water is taken, and the prescription amount of carbomer, glycerol and 1,2-pentanediol is slowly added under stirring condition, and dissolved at 40℃ for 6h;

[0067] (2) The prescription amount of triethanolamine is added to the solution of step (1), and dissolved and stirred for 0.5h, and used;

[0068] (3) The prescription amount of natogel and pullulan is added to the remaining 40% of the prescription amount of purified water, and dissolved at 60℃ for 1h, and then the prescription amount of recombinant humanized type III collagen is added, and stirred for 1h;

[0069] (4) The solution of step (3) is added to the solution of step (2), and dissolved at 40℃ for 3h, and then the prescription amount of phenoxyethanol is added and dissolved and stirred for 1h, to prepare the gel.

[0070] Example 4

[0071] A gel with skin improvement and whitening effect, the raw material composition and content are shown in Table 1, its preparation method comprises the following steps:

[0072] (1) 60% of the prescription amount of purified water is taken, and the prescription amount of carbomer, glycerol and 1,2-pentanediol is slowly added under stirring condition, and dissolved at 40℃ for 6h;

[0073] (2) To the solution of step (1), add the prescribed amount of triethanolamine, dissolve and stir for 0.5 h, and stand by;

[0074] (3) To the remaining 40% of the prescribed amount of purified water, add the prescribed amount of natolac gum and pullulan, stir and dissolve at 60°C for 1 h, then add the prescribed amount of recombinant humanized type III collagen, and continue to stir for 1 h;

[0075] (4) Add the solution of step (3) to the solution of step (2), stir and dissolve at 40°C for 3 h, add the prescribed amount of phenoxyethanol, stir and dissolve for 1 h, to prepare a gel.

[0076] Comparative Example 1

[0077] A gel. The raw material components and contents are shown in Table 1. It differs from Example 2 in that there is no natolac gum and pullulan. The preparation method comprises the following steps:

[0078] (1) Take 60% of the prescribed amount of purified water, slowly add the prescribed amount of carbomer, glycerol, and 1,2-pentanediol under stirring conditions, and stir and dissolve at 40°C for 7 h;

[0079] (2) To the solution of step (1), add the prescribed amount of triethanolamine, dissolve and stir for 0.7 h, and stand by;

[0080] (3) To the remaining 40% of the prescribed amount of purified water, add the prescribed amount of recombinant humanized type III collagen, and continue to stir for 0.7 h;

[0081] (4) Add the solution of step (3) to the solution of step (2), stir and dissolve at 40°C for 3 h, add the prescribed amount of phenoxyethanol, stir and dissolve for 0.5 h, to prepare a gel.

[0082] Comparative Example 2

[0083] A gel. The raw material components and contents are shown in Table 1. It differs from Example 2 in that there is no pullulan. The preparation method comprises the following steps:

[0084] (1) Take 60% of the prescribed amount of purified water, slowly add the prescribed amount of carbomer, glycerol, and 1,2-pentanediol under stirring conditions, and stir and dissolve at 40°C for 7 h;

[0085] (2) To the solution of step (1), add the prescribed amount of triethanolamine, dissolve and stir for 0.7 h, and stand by;

[0086] (3) To the remaining 40% of the prescribed amount of purified water, add the prescribed amount of natolac gum, stir and dissolve at 60°C for 2 h, then add the prescribed amount of recombinant humanized type III collagen, and continue to stir for 0.7 h;

[0087] (4) The solution of step (3) is added to the solution of step (2), stirred and dissolved at 40℃ for 3h, and the prescribed amount of phenoxyethanol is added and stirred and dissolved for 0.5h to prepare a gel.

[0088] Comparative Example 3

[0089] A gel, whose raw material composition and content are shown in Table 1, differs from Example 2 in that there is no natrosol, and its preparation method comprises the following steps:

[0090] (1) 60% of the prescribed amount of purified water is taken, and the prescribed amount of carbomer, glycerol, and 1,2-pentanediol is slowly added under stirring conditions, and stirred and dissolved at 40℃ for 7h;

[0091] (2) The prescribed amount of triethanolamine is added to the solution of step (1), stirred and dissolved for 0.7h, and reserved;

[0092] (3) The prescribed amount of pullulan is added to the remaining 40% of the prescribed amount of purified water, stirred and dissolved at 60℃ for 2h, and the prescribed amount of recombinant humanized type III collagen is added and stirred for 0.7h;

[0093] (4) The solution of step (3) is added to the solution of step (2), stirred and dissolved at 40℃ for 3h, and the prescribed amount of phenoxyethanol is added and stirred and dissolved for 0.5h to prepare a gel.

[0094] Comparative Example 4

[0095] A gel, whose raw material composition and content are shown in Table 1, differs from Example 2 in that there is no recombinant humanized type III collagen, and its preparation method comprises the following steps:

[0096] (1) 60% of the prescribed amount of purified water is taken, and the prescribed amount of carbomer, glycerol, and 1,2-pentanediol is slowly added under stirring conditions, and stirred and dissolved at 40℃ for 7h;

[0097] (2) The prescribed amount of triethanolamine is added to the solution of step (1), stirred and dissolved for 0.7h, and reserved;

[0098] (3) The prescribed amount of natrosol and pullulan is added to the remaining 40% of the prescribed amount of purified water, stirred and dissolved at 60℃ for 2h;

[0099] (4) The solution of step (3) is added to the solution of step (2), stirred and dissolved at 40℃ for 3h, and the prescribed amount of phenoxyethanol is added and stirred and dissolved for 0.5h to prepare a gel.

[0100] Comparative Example 5

[0101] A gel, raw material composition and content are shown in Table 1, which is different from Example 2 in that the Natrosol is replaced with an equal amount of guar gum, and its preparation method comprises the following steps:

[0102] (1) Take 60% of the prescription amount of purified water, slowly add the prescription amount of carbomer, glycerol, 1,2-pentanediol under stirring condition, and dissolve at 40℃ for 7h;

[0103] (2) Add the prescription amount of triethanolamine to the solution of step (1), dissolve and stir for 0.7h, and wait for use;

[0104] (3) Add the prescription amount of guar gum and pullulan to the remaining 40% of the prescription amount of purified water, dissolve at 60℃ for 2h, then add the prescription amount of recombinant humanized type III collagen, continue to stir for 0.7h;

[0105] (4) Add the solution of step (3) to the solution of step (2), dissolve at 40℃ for 3h, add the prescription amount of phenoxyethanol and stir for 0.5h to prepare the gel.

[0106] Comparative Example 6

[0107] A gel, raw material composition and content are shown in Table 1, which is different from Example 2 in that there is no recombinant humanized type III collagen and pullulan, and its preparation method comprises the following steps:

[0108] (1) Take 60% of the prescription amount of purified water, slowly add the prescription amount of carbomer, glycerol, 1,2-pentanediol under stirring condition, and dissolve at 40℃ for 7h;

[0109] (2) Add the prescription amount of triethanolamine to the solution of step (1), dissolve and stir for 0.7h, and wait for use;

[0110] (3) Add the prescription amount of Natrosol to the remaining 40% of the prescription amount of purified water, dissolve at 60℃ for 2h;

[0111] (4) Add the solution of step (3) to the solution of step (2), dissolve at 40℃ for 3h, add the prescription amount of phenoxyethanol and stir for 0.5h to prepare the gel.

[0112] Comparative Example 7

[0113] A gel, raw material composition and content are shown in Table 1, which is different from Example 2 in that there is no recombinant humanized type III collagen and pullulan, and its preparation method comprises the following steps:

[0114] (1) Take 60% of the prescription amount of purified water, slowly add the prescription amount of carbomer, glycerol, 1,2-pentanediol under stirring condition, and dissolve at 40℃ for 7h;

[0115] (2) To the solution of step (1), add the prescribed amount of recombinant humanized type III collagen, natrosol, pullulan, and stir to dissolve at 60°C for 2.7 h;

[0116] (3) To the solution of step (2), add the prescribed amount of triethanolamine, and stir to dissolve for 0.7 h, and stand by;

[0117] (4) To the solution of step (3), add the prescribed amount of phenoxyethanol, and stir to dissolve for 0.5 h, to prepare a gel.

[0118] Comparative Example 8

[0119] A gel, which has the same raw material components and contents as in Example 2, but has a different preparation method, the preparation method comprising the following steps:

[0120] (1) Take 60% of the prescribed amount of purified water, and slowly add the prescribed amounts of carbomer, glycerol, and 1,2-pentanediol under stirring conditions, and stir to dissolve at 40°C for 7 h;

[0121] (2) To the remaining 40% of the prescribed amount of purified water, add the prescribed amounts of natrosol and pullulan, and stir to dissolve at 60°C for 2 h, and then add the prescribed amount of recombinant humanized type III collagen, and continue stirring for 0.7 h;

[0122] (3) Add the solution of step (2) to the solution of step (1), and stir to dissolve at 40°C for 3 h;

[0123] (4) To the solution of step (3), add the prescribed amount of triethanolamine, and stir to dissolve for 0.7 h, and stand by;

[0124] (5) Add the solution of step (4) to the prescribed amount of phenoxyethanol, and stir to dissolve for 0.5 h, to prepare a gel.

[0125] Comparative Example 9

[0126] A gel, which has the same raw material components and contents as in Example 2, but has a different preparation method, the preparation method comprising the following steps:

[0127] (1) Take 60% of the prescribed amount of purified water, and slowly add the prescribed amounts of carbomer, glycerol, and 1,2-pentanediol under stirring conditions, and stir to dissolve at 40°C for 7 h;

[0128] (2) To the solution of step (1), add the prescribed amount of triethanolamine, and stir to dissolve for 0.7 h, and stand by;

[0129] (3) Add the prescription amount of budding short stem enzyme polysaccharide to the remaining 40% of the prescription amount of purified water, stir and dissolve at 60°C for 2 hours, then add the prescription amount of recombinant humanized type III collagen, and continue stirring for 0.7 hours;

[0130] (4) Add the solution from step (3) to the solution from step (2), stir and dissolve at 40°C for 3 hours, add the prescribed amount of phenoxyethanol and stir and dissolve for 0.5 hours to prepare a gel.

[0131] Comparative Example 10

[0132] A gel, the composition and content of its raw materials are shown in Table 1, differs from Example 2 in that the content of recombinant humanized type III collagen is increased, while the content of natopor and budding short stalk polysaccharide is decreased.

[0133] The preparation method of the gel described above is exactly the same as that in Example 2.

[0134] Experimental Example 1: Observation of Characteristics

[0135] The gels prepared in Example 2 and Comparative Examples 7 and 8 are as follows Figure 1 As shown, the gel prepared in Example 2 was colorless and transparent with good properties; the gels prepared in Comparative Examples 7 and 8 were cloudy with some precipitated substances. These results indicate that adding recombinant humanized type III collagen before triethanolamine during gel preparation leads to instability in the gel system. Adding recombinant humanized type III collagen after triethanolamine, especially after the neutralization of triethanolamine and carbomer, makes the gel system more stable.

[0136] Example 2: Viscosity Index Test

[0137] The gels prepared in Examples 1-3 and Comparative Examples 1-7 were tested according to Method 1 of General Chapter 0633 "Viscosity Determination" in Part IV of the Pharmacopoeia of the People's Republic of China (2020 Edition). The test results are shown in the table below:

[0138] Table 2. Kinematic viscosity of gels, unit: mm 2 / s

[0139]

[0140] Considering that the viscosity of the gel can affect the verification experiments on skin improvement and whitening efficacy, in order to reduce the existence of variables and ensure that the gel remains on the application sites of volunteers consistently, the kinematic viscosity of the gel system was kept as close as possible to 8000-10000 mmHg when designing the implementation and comparative examples by varying the amount of thickener. 2 Within the range of / s.

[0141] From the test results of Examples 1-4, it can be seen that the kinematic viscosity of the gel can be kept approximately the same by reducing the content of carbomer while increasing the content of natrosol. From the comparison of the test results of Comparative Example 3 and Example 2, it can be seen that when the gel does not contain natrosol in the raw material composition, the kinematic viscosity of the gel is significantly reduced. In combination with the test results of Comparative Example 9, it can be seen that when the raw material composition does not contain natrosol but the content of carbomer is increased, the kinematic viscosity of the gel can be increased. Or in combination with the test results of Comparative Example 5, it can be seen that the kinematic viscosity of the gel can also be restored by replacing natrosol with guar gum. In summary, carbomer, natrosol and guar gum can all increase the viscosity of the gel system, and all can be used as thickening agents.

[0142] Example 3: Tape stripping experiment

[0143] Experimental method: A total of 60 volunteers were recruited for the tape stripping experiment, 5 people in each group, a total of 12 groups. The gels prepared in Examples 1-4 and Comparative Examples 1-6 and Comparative Examples 9-10 were numbered as 1#-12# in turn, and were applied to 12 groups of volunteers for experiment.

[0144] The test area was the flexor side of the left and right forearms. Before the test, the test site was washed with clean water, and the test site was balanced for 30 min in the experimental environment (environmental temperature was 21±1℃, relative humidity was 50±1%RH); then the CorneofixR tape was pasted on the test area, pressed 3-10 times, and then quickly torn off to remove the cutin, and a tape stripping damage model was established. After the damage was generated, the TEWL value of the test area was measured. Randomly selected test and control areas, one side of the test area was applied with the gel sample (test group), and the gel sample was applied to the test area at a dosage of (2.0±0.1) mg / cm 2 The other side of the test area was not treated (control group). After the sample was applied, the timing started, and the TEWL value of the test area was measured again after 0.5 h, 1.5 h and 24 h of gel sample use. The change of TEWL value at different visit time points was used to represent the repair effect of the gel product, and the smaller the TEWL value, the smaller the trans-epidermal water loss of the skin, and the more obvious the improvement of the skin.

[0145] Specifically, the method for establishing the tape stripping damage model was as follows: the CorneofixR tape was pasted on the test area, pressed 3-10 times, and then quickly torn off to remove the cutin, and the trans-epidermal water loss (TEWL) was measured. This TEWL value was taken as the initial TEWL value. The difference in TEWL value was the difference between the visit time point TEWL value and the initial TEWL value.

[0146] Experimental results: The TEWL difference values of the test area at 0.5 h, 1.5 h and 24 h are as follows: Figure 2 , Figure 2 and Figure 4The data analysis showed that the TEWL difference of the gel samples of Examples 1-4 was significantly greater than that of Comparative Examples 1-6 and 9, indicating that the gel samples of Examples 1-4 had more obvious skin improvement effect. After using the gel samples of Examples 1-4, the skin condition could be quickly improved, the skin was quickly moisturized, and the skin of the test site had a sustained protection and improvement effect. After 24 hours, the skin of the test site using the gel samples of Examples 1-4 recovered better than that using the gel samples of Comparative Examples 1-6 and 9.

[0147] From the gel use results of Example 2 and Comparative Examples 1 / 2 / 3, and Example 2 and Comparative Examples 2 / 4 / 6, it was known that Natrosol and recombinant humanized type III collagen and short-spore enzyme polysaccharide had a synergistic effect on improving skin condition.

[0148] From the gel use results of Comparative Examples 3 / 5 / 9, it was known that the TEWL difference of the three was basically the same, indicating that guar gum and carbomer had no contribution to skin repair in the gel sample.

[0149] From the gel use results of Comparative Example 10, it was known that further increasing the content of recombinant humanized type III collagen did not show obvious improvement in skin repair effect, but increased the production cost of the gel.

[0150] Example 4: Lactic acid stinging test

[0151] Experimental method: A total of 60 volunteers were recruited for this lactic acid stinging test, and the recruitment standard was based on T / BDCA 0005-2023 Human Lactic Acid Stinging Test for Cosmetics Soothing Effect Test Method, with 5 people in each group, a total of 12 groups. The gels prepared from Examples 1-4 and Comparative Examples 1-6 and Comparative Examples 9-10 were numbered 1#-12# in turn, and were applied to 12 groups of volunteers for experiments.

[0152] Cleanse the face with cleansing honey and water, dry, and balance for 30 minutes in the experimental environment (environmental temperature is 21±1℃, relative humidity is 50±1%RH). At room temperature, 10% lactic acid solution about 50μL was applied to the nasolabial fold and one side of the cheek, with a test area of about 0.8mm×0.8mm. The side with the above lactic acid application was used as the gel sample application site, with a dosage of about 0.3g, and the other side was the control group without treatment. The subjects' subjective symptoms were inquired at 2.5min and 5min. The scoring was based on a 4-point scale (0 points for no stinging, 1 point for mild stinging, 2 points for moderate stinging, and 3 points for severe stinging), and ≥3 points were considered positive for lactic acid stinging reaction.

[0153] Experimental results: The lactic acid stinging scores at 2.5min and 5min were as follows: Figure 5 and Figure 6The pain feeling of the tested part of the gel samples of Examples 1-4 was obviously weaker than that of the tested part of the gel samples of Comparative Examples 1-6, 9-10 according to the statistical results of the inquiry. The gel products of Examples 1-4 had soothing effect on the skin irritation caused by lactic acid.

[0154] According to the statistical results of Comparative Examples 3 / 5 / 9, guar gum and carbomer had no contribution to soothing irritation of the gel samples. According to the statistical results of Comparative Example 10, further increasing the content of recombinant humanized type III collagen did not show obvious improvement in the effect of soothing irritation, but increased the production cost of the gel.

[0155] Experimental Example 5: Tyrosinase activity inhibition experiment

[0156] Experimental method: in a 96-well plate, the experiment was divided into two groups, experimental group: adding 40 μL of the sample solution to be tested (solvent: purified water) and 60 μL of 250 U / mL tyrosinase solution, control group: adding 40 μL of 80 mM PBS buffer (pH 6.8) and 60 μL of 250 U / mL tyrosinase solution; 3 replicate wells for each treatment; the 96-well plate was incubated at room temperature (20-25°C) for 10 min, then 100 μL of 6.0 mM tyrosine solution was added to each well, shaken and mixed, and then placed in a 37°C incubator for reaction for 30 min. The absorbance value at 475 nm wavelength was measured by using an enzyme marker.

[0157] Table 3. Components and contents in the sample to be tested and the corresponding absorbance values

[0158]

[0159]

[0160] Experimental results: the volume ratio of the sample solution, tyrosine solution and tyrosinase solution is 2:5:3, the inhibition effect of the sample on the activity of tyrosinase is negatively correlated with the color of the reaction system, that is, the darker the color, the worse the inhibition effect of the sample on the activity of tyrosinase, and the inhibition effect of the sample on the activity of tyrosinase is characterized by detecting the absorbance value at 475 nm. The experimental results are shown in Table 3. The absorbance value of the reaction solution containing only tyrosine and tyrosinase is 0.423. As shown in Table 3, the inhibition effect of the sample No. 6 on the activity of tyrosinase is obviously better than that of the samples No. 1-4, indicating that the inhibition effect of the combination of the three active substances on the activity of tyrosinase is obviously better than that of the combination of one or two active substances, and the three active substances have a synergistic effect on the inhibition of the activity of tyrosinase. At the same time, the inhibition effect of the samples No. 5-7 on the activity of tyrosinase is obviously better than that of the samples No. 1-4, further indicating that the active substances in the samples No. 5-7 have a synergistic effect.

[0161] Example 6: Whitening effect test

[0162] 48 volunteers aged 20-50 years old with healthy skin, no photosensitivity disease and no allergy history were selected for effect test. The gels prepared in Examples 1-4 and Comparative Examples 1-6 and Comparative Examples 9-10 were numbered as 1#-12# in turn, and were applied to the volunteers for experiment.

[0163] Experimental method: In a constant environment of room temperature 21±1℃ and relative humidity 50±10%, the relevant data were tested after the subjects sat still for 30 min, and the test results were averaged. The specific process: the volunteers were randomly divided into 6 groups, 8 people in each group, the first group applied 1# gel on the fixed position of the inner side of the left arm, and 2# gel on the same position of the right arm, the second group applied 3# gel on the fixed position of the inner side of the left arm, and 4# gel on the same position of the right arm, the third group applied 5# gel on the fixed position of the inner side of the left arm, and 6# gel on the same position of the right arm, the fourth group applied 7# gel on the fixed position of the inner side of the left arm, and 8# gel on the same position of the right arm, the fifth group applied 9# gel on the fixed position of the inner side of the left arm, and 10# gel on the same position of the right arm, and the sixth group applied 11# gel on the fixed position of the inner side of the left arm, and 12# gel on the same position of the right arm; twice a day, the amount of sample applied was 2.00±0.05 mg / cm 2 , the sample application area should not be less than 6 cm 2 , once in the morning and once in the evening, after application, moderate massage was performed, and continuous use was carried out for 8 weeks, during which the melanin content of the arm application position of the volunteers was tested every two weeks.

[0164] The method for testing the melanin content: the MI value of each test area was measured by the skin melanin detector, each test area was tested three times, and the results were recorded; the smaller the MI value, the lower the skin melanin content, and vice versa.

[0165] The melanin content change value (ΔM) = M0-M X , wherein M0 is the initial MI value, M X is the MI value after using the product X for days.

[0166] Experimental results: the melanin content change values at the 2nd week, the 4th week, and the 8th week are shown in Table 4 and Figure 7 , Figure 8 , Figure 9 .

[0167] Table 4. Statistical results of the melanin content change values after using the gel

[0168]

[0169] The melanin content change values of the volunteers using the gel samples of Examples 1-4 were significantly higher than those of the volunteers using the gel samples of Comparative Examples 1-6 and 9. Among them, the melanin content change values of the volunteers using the gel sample of Example 2 were significantly higher than those of the volunteers using the gel samples of Examples 1-4, and it can be seen from the results of using the gel samples of Example 2 and Comparative Examples 1 / 2 / 3 and Example 2 and Comparative Examples 2 / 4 / 6 that Natol gel and recombinant humanized type III collagen and short-spore polysaccharide exhibit a synergistic effect on whitening and freckle removal.

[0170] The melanin content change values of the volunteers using the gel sample of Comparative Example 5 were significantly smaller than those of the volunteers using the gel sample of Example 2, and were almost the same as those of the volunteers using the gel sample of Comparative Example 3, and the melanin content change values of the volunteers using the gel samples of Comparative Examples 3 and 9 were basically the same, indicating that guar gum and carbomer do not affect the change of melanin and have no contribution to the whitening and freckle removal of the gel sample.

[0171] The melanin content change values of the volunteers using the gel sample of Comparative Example 10 were not significantly different from those of the volunteers using the gel samples of Examples 1-4, indicating that after the content of recombinant humanized type III collagen exceeds 0.5%, further increasing the content of recombinant humanized type III collagen will not significantly reduce the melanin content. Therefore, when preparing the gel, the amount of recombinant humanized type III collagen can be reduced, and the amount of Natol gel and short-spore polysaccharide can be increased to achieve a similar effect, thereby reducing the production cost.

Claims

1. A gel having skin-improving and whitening effects, characterized by, The raw materials include, in percentage by mass: 0.1%-0.5% of recombinant humanized type III collagen, 0.2%-1% of pullulan, 0.2%-0.6% of Natrosol, 0.1%-0.5% of carbomer, 0.1%-0.5% of triethanolamine, 1%-4% of 1,2-pentanediol, 0.1%-3% of glycerol, 0.1%-3% of phenoxyethanol, and the balance of water; the amino acid sequence of the recombinant humanized type III collagen is shown as SEQ ID NO. 1; The gel with skin improvement and whitening effects is prepared according to the following steps: (1) A part of the prescription amount of purified water is slowly added to the prescription amount of carbomer, glycerol and 1,2-pentanediol under stirring condition, and dissolved at 40-60℃; (2) The prescription amount of triethanolamine is added to the solution of step (1) and dissolved under stirring, and used after stirring; (3) The prescription amount of Natrosol and pullulan is added to the remaining prescription amount of purified water, dissolved under stirring at 40-60℃, and then the prescription amount of recombinant humanized type III collagen is added and continuously stirred; (4) The solution of step (3) is added to the solution of step (2), dissolved under stirring at 40-60℃, and then the prescription amount of phenoxyethanol is added and dissolved under stirring to prepare the gel.

2. The gel with skin-improving and whitening effects as described in claim 1, characterized in that, The part of the prescription amount of purified water in step (1) is 60%-80% of the prescription amount of purified water.

3. The gel with skin-improving and whitening effects as described in claim 1, characterized in that, The time for stirring and dissolving in step (1) is 5-7h.

4. The gel with skin-improving and whitening effects as described in claim 1, characterized in that, The time for stirring and dissolving in step (2) is 0.5-1h.

5. The gel having skin improvement and whitening efficacy according to claim 1, wherein the gel is prepared by mixing the components (a) to (d) in the following order: (a) (b) (c) (d). The time for stirring and dissolving in step (3) is 1-3h.

6. The gel having skin improvement and whitening efficacy according to claim 1, wherein the gel is prepared by adding the following components to water and then stirring the mixture at 60°C for 1 hour to 2 hours. The time for continuously stirring in step (3) is 0.5-1h.

7. The gel with skin-improving and whitening effects as described in claim 1, characterized in that, The time for stirring and dissolving at 40-60℃ in step (4) is 1-3h.

8. The gel with skin-improving and whitening effects as described in claim 1, characterized in that, The time for stirring and dissolving after adding the prescription amount of phenoxyethanol in step (4) is 0.5-1.5h.

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