A method for domesticating mandarin fish using large-scale loach as transit bait

Abstract

The present invention provides a method for domesticating mandarin fish using large-scaled paraloach as transit bait, and belongs to the field of freshwater fish seed breeding. The mandarin fish domestication method provided by the present invention goes through four stages: starvation → live bait feeding → transit feeding → feed, and the transit feeding of mandarin fish involved includes: fresh dead loach coated with powder → fresh dead loach coated with powder + ice-fresh loach coated with powder → ice-fresh loach coated with powder + feed. The present invention uses loach as transit bait, which not only cleverly caters to the mandarin fish's unique visual response mechanism for feeding, but also significantly improves the feeding efficiency during the feed conversion process, makes full use of the loach's flexible body shape and ability to swim quickly in the water, and perfectly matches the mandarin fish seedlings' extremely high desire to prey on dynamic and active small aquatic organisms, opening up a new efficient and environmentally friendly way for the feed domestication of mandarin fish seedlings. The domestication procedure is highly replicable, with low loss of mandarin fish fry, high domestication rate, few diseases, fast growth of mandarin fish fry and excellent quality.

Description

Technical Field

[0001] The invention relates to the field of freshwater fish seed breeding, and in particular to a method for domesticating mandarin fish using large-scale loach as transit bait. Background Art

[0002] Mandarin fish is one of the freshwater aquaculture fish with the most promising market prospects in my country. It has a complete industrial chain, but the total output is not high, and the market has always been in a state of supply exceeding demand. At present, mandarin fish farming is mainly limited by the supporting farming of live bait fish. When it is artificially cultivated, it requires 4-5 times the area of ​​water to cultivate bait fish, which limits the large-scale intensive farming of mandarin fish.

[0003] At present, a small number of mandarin fish fry can be successfully domesticated in aquariums, but the survival rate of large-scale domestication of mandarin fish fry is not high, resulting in a serious shortage of edible mandarin fish fry. The output of edible mandarin fish accounts for less than 1% of the total output of mandarin fish, and the survival rate of domesticated fry is not high, and the size of individuals is uneven, which directly affects the later breeding production efficiency.

[0004] In addition, the following factors also contribute to the low survival rate of mandarin fish: (1) At present, mandarin fish is usually raised with fry of fish such as dace, silver carp, bighead carp, and red-eyed trout. These fry are frozen and inactivated, and a large amount of water loss during thawing causes them to rot and deteriorate very quickly. On the one hand, this causes the water quality for domestication to deteriorate, and on the other hand, the nutritional value of rotten and deteriorated fresh frozen fry is greatly reduced, causing the physical condition of the domesticated mandarin fish fry to deteriorate, grow slowly, and be prone to outbreaks of group diseases. This is also an important reason for the low survival rate of mandarin fish fry in current domestication. (2) Ordinary transition baits and mandarin fish feeds are very different in morphology. The transition process causes a large change in the gastric motility of mandarin fish, resulting in a slow feed adaptation process and easy to cause gastrointestinal histological damage to mandarin fish. Summary of the invention

[0005] In view of this, in order to improve the survival rate and seedling quality of large-scale domestication of mandarin fish fry and promote the development of the mandarin fish industry towards intensive development, the present invention adopts large-scale loach seedlings as re-export bait in the domestication of mandarin fish seedling feed, and can obtain high-quality mandarin fish seedlings for feeding in batches.

[0006] In order to achieve the above effects, the present invention adopts the following technical solutions:

[0007] The present invention provides a method for domesticating mandarin fish using large-scale loach as transit bait, and the method comprises the following steps:

[0008] S1. On the first day of acclimation, the mandarin fish fry were starved;

[0009] S2. On the 2nd to 4th day of acclimatization, live bait feeding is carried out: live loach is fed once in the morning and evening every day, and 0.05kg is fed for every kilogram of mandarin fish. The seedlings are screened, parasites are examined under a microscope, disinfected, and the feeding situation is checked. The seedlings with healthy physique and normal feeding are used for the next step. The focus of this link is to ensure that the mandarin fish seedlings are healthy and adapt to the cultivation environment;

[0010] S3, Transit feeding:

[0011] S3.1 On the 5th to 9th day of acclimation, feed fresh dead loach coated with powder once in the morning and evening, 0.05kg per kilogram of mandarin fish, cultivate feeding rhythm, microscopically examine parasites, monitor water quality, 90% of mandarin fish fry are full, and fry that do not eat dead bait are eliminated. The key points of this link are: screening and eliminating fry that do not eat dead bait, strengthening rhythm, promoting clustering for food, and preventing diseases;

[0012] S3.2 On the 10th to 12th day of domestication, feed fresh dead loach powder and ice fresh loach powder once in the morning and evening, 0.06kg per kilogram of mandarin fish, cultivate feeding rhythm, microscopically examine parasites, monitor water quality, and 90% of mandarin fish fry are full. Then, sieve and grade them for the next step of domestication. The key points of this link are: strengthening rhythm, grabbing food state, and adapting to the shape and smell of feed;

[0013] S3.3 On the 13th to 15th day of acclimation, feed fresh loach powder and feed once in the morning and evening. The base amount of feeding per kilogram of mandarin fish starts from 0.06kg of fresh loach powder. On the 13th, 14th and 15th days, the proportion of pellet feed is increased by 20%, 50% and 80% respectively. Cultivate feeding rhythm, examine parasites under a microscope, monitor water quality, and make mandarin fish fry 90% full. The key points of this link are: strengthening rhythm, grabbing food state, and adapting to feed;

[0014] S3.4 On the 16th to 21st day of domestication, feed is fed and the feeding amount is increased by 0.01kg of feed per kilogram of mandarin fish per day; feeding rhythm is cultivated, parasites are examined under a microscope, water quality is monitored, and when the satiety rate of feed intake reaches more than 85%, the finished feed mandarin fish can be cultivated. The key points of this link are: to strengthen and stabilize feed feeding, prevent the phenomenon of regurgitation, and prepare for the finished feed mandarin fish.

[0015] Furthermore, step S2 uses 0.3 mg / L povidone iodine for disinfection.

[0016] Furthermore, in step S3.2, the ratio of the fresh dead loach coated with flour to the chilled loach coated with flour is 1:1.

[0017] Furthermore, before the domestication, the relevant facilities are disinfected, a feeding platform is built to provide shade, and a feeding attractant lighting device is installed in the breeding cage.

[0018] Furthermore, one week before the start of domestication, the floating pond mouth is disinfected with quicklime, and the amount of quicklime is 100kg / mu; a plastic shed is set on the floating raft, and black shade nets are used on all sides for shading; each cage is provided with a duckbill water outlet, with an inner diameter of 16mm and a width of 7.55cm; a feed and storage platform is built; the cage is placed in the floating raft and fixed one week in advance. The cage is 3m long, 2m wide and 2m deep. A 15cm long blue oval lamp is placed on each side of the bottom of the cage as an attractant light device; the blue oval lamp is an attractant device, 15cm long, with an upper and lower radius of 3cm, and is turned on 10 minutes before each feeding.

[0019] Furthermore, the mandarin fish fry used for domestication in step S1 are pre-roughened to a size of 6±0.5 cm.

[0020] Furthermore, the mandarin fish fry described in step S1 are subjected to roughing treatment in the earthen pond for one month, and the caught mandarin fish have a body length of 6.44±0.59cm, a body weight of 11.12±2.10g, and a mouth fissure of 0.91±0.12cm. 20,000 to 22,000 mandarin fish can be harvested from every acre of pond; loach and water flower fry are placed in the earthen pond used for cultivating mandarin fish fry 20 days in advance, soybean milk is used to fertilize the water every other day, and loaches are fed at regular intervals and at fixed points after dark using a loach attractant to ensure sufficient bait fish after the mandarin fish fry are put into the pond.

[0021] Furthermore, the loach described in step S2 is Paraloach sphaerocephalus, whose unique long strip shape is highly similar to the shape of the feed.

[0022] Furthermore, the preparation process of the large-scaled loach seedlings is as follows: a 5-meter-long ground cage is placed every 10 meters in the loach breeding pond in the evening every day, and the loaches in the ground cages are collected at 5 o'clock the next morning; a sufficient amount of loaches with a body length of 3.7±0.25cm are caught as re-export bait fish for the domestication of mandarin fish, and placed in an indoor temporary holding pond, and an aeration device is added to the bottom; the aeration device is a 3-kilowatt impeller aerator, and one unit is equipped for every 3 mu.

[0023] Furthermore, the fresh dead loach coated with powder refers to the dead loach mixed with mandarin fish feed, and the mandarin fish feed is a special feed for Jie Da mandarin fish; mandarin fish feed No. 2, crude protein 52%, particle size 2.5mm, length 1.5-2cm; before each feeding, the following treatment is performed: soak in water for 7 minutes, dry for 3 minutes, rinse with water once, and vacuum process with an XT-400D single-chamber vacuum packaging machine.

[0024] After the treatment, a long strip of feed is obtained. The long strip of feed is in a floating state under the stimulation of water flow in the water body. After the water flow stops, the long strip of feed will be suspended in the water body without sinking, thereby prolonging the time for the mandarin fish to ingest the feed.

[0025] The powder used in the domestication process is Jie Da fish fry starter powder, which has a protein content of 45% and is in powder form, which can enhance the fish fry's ability to resist stress.

[0026] Furthermore, the purpose of coating the loach with powder is to make the mandarin fish familiar with the smell of feed in advance.

[0027] Furthermore, anti-stress treatment is performed during the domestication process, preferably using VC astaxanthin at a dosage of 30 g / mu.

[0028] Furthermore, the cultivation of feeding rhythm is specifically carried out after 10 minutes of water flow stimulation, the water flow rate is 0.8m / s, the nozzle is 30cm away from the water surface, and the water flow contacts the water surface at an angle of 45° during operation; when feeding, the mandarin fish fry gather at the water flow, forming a food-grabbing behavior, and each feeding time is 1.5 hours.

[0029] The mandarin fish domestication method provided by the invention goes through four stages: starvation→feeding with live bait→transshipment feeding→feed, and the involved transshipment feeding of mandarin fish includes: fresh dead loach coated with powder→fresh dead loach coated with powder+frozen loach coated with powder→frozen loach coated with powder+feed, and the mandarin fish that is difficult to domesticate is subjected to secondary domestication operation during the domestication process.

[0030] Mandarin fish that have been domesticated for three weeks can be fed using a feed feeding machine to save manpower; with the help of an automatic feeding machine, the finished product of feed mandarin fish can be cultivated, and the feed machine should be checked regularly to see if there is enough feed; the ammonia nitrogen content, dissolved oxygen and pH value of the water body should be tested every other day; a mandarin fish in poor condition should be examined under a microscopic microscope every day, and gill sections should be made for microscopic observation of parasites.

[0031] The feed acclimation of mandarin fish fry took a total of 21 days. The initial body length, weight, number and other indicators of mandarin fish fry were recorded before the start of each batch of acclimation. The indicators were measured again at the end of the acclimation and the acclimation rate was obtained.

[0032] Compared with the prior art, the present invention has the following beneficial effects:

[0033] (1) The feeding method provided by the present invention can prey on loach seedlings with a body length of 4 cm or less (including 4 cm), and the loach seedlings have less body water loss after being frozen and thawed, are not easy to rot, and are easy to be eaten by the mandarin fish. After being eaten, they form a curled state in the stomach of the mandarin fish, which is easy for the mandarin fish to digest; they are easy to suspend in water, have a high predation rate, and have a short cylindrical body shape similar to the shape of later granular feed, which has a good feeding effect. The nutritional content of loach is very high, so using large-scaled paraloach as a transit bait fish can significantly increase the survival rate of mandarin fish training to more than 85%, and the trained mandarin fish seedlings grow fast, have good physique, and have less disease. The method of the present invention significantly increases the feeding rate of mandarin fish seedlings, increases their growth rate, and enhances their disease resistance.

[0034] (2) The present invention utilizes the high adaptability of loach and mandarin fish in breeding time, and can provide transit bait fish for annual mandarin fish domestication, ensure the annual output of mandarin fish fry, and promote the development of facility-based and intelligent mandarin fish farming. Establishing a mandarin fish domestication mechanism using loach as transit bait can provide a large amount of transit bait fish, transition from ice-fresh bait to full feed, and stably ingest feed for a long time without regurgitation.

[0035] (3) The present invention regularly screens the domesticated mandarin fish fry to avoid cannibalism due to hunger; the domestication is carried out on a floating raft with a large water surface, which solves the problem of waste disposal during the domestication of mandarin fish; the floating raft can adjust the water level of the net cage according to the water level and always maintain it at a depth of 2m. After feeding, the mandarin fish returns to the bottom of the net cage, which satisfies the mandarin fish's deep-water living habits and avoids the stress of high water temperature in summer on the mandarin fish, thereby ensuring that the survival rate of the domesticated mandarin fish fry reaches more than 85%.

[0036] (4) After the mandarin fish has been tamed for 3 weeks through the taming procedure of the present invention, an automatic feeding device can be directly added to the net cage, which can be safely cultured for a long time, saving manpower and improving the efficiency of mandarin fish culture.

[0037] (5) The domestication procedure provided by the present invention using loach as re-export bait is highly reproducible, with low loss of mandarin fish seedlings, high domestication rate, few diseases, fast growth of mandarin fish seedlings, and excellent quality. DETAILED DESCRIPTION

[0038] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention.

[0039] The experimental methods or test methods described in the following examples are conventional methods unless otherwise specified; the raw materials and auxiliary agents are obtained from conventional commercial channels or prepared by conventional methods unless otherwise specified.

[0040] Example 1

[0041] (1) Preparation before floating raft acclimation

[0042] On August 23, 2023, the pond where the floating raft is located is disinfected and domesticated with quicklime one week in advance, with a dosage of 100kg / mu; a plastic greenhouse is set up on the floating raft to shade the cages, and black shade nets are used around the floating raft for shading; each cage is equipped with a water inlet with an inner diameter of 1.6cm and a width of 7.55cm; a feed and storage platform is built; the net is placed in the floating raft and fixed one week in advance. The cage is 3m long, 2m wide and 2m deep. A 15cm long blue oval lamp is placed on each side of the bottom of the cage as a light attractant.

[0043] (2) Preparation of Mandarin Fish Fry

[0044] The mandarin fish breeding pond is a rectangular earthen pond of 670m2, with a depth of 2.5-3.0m and equipped with bird-proof nets; the pond bottom is flat, slightly inclined toward the drain outlet, and the irrigation and drainage system is complete; on August 31, 2023, the mandarin fish were marked in the earthen pond for one month, and the length when entering the pond was 2.5±0.25cm. Mandarin fish with a body length of 6.44±0.59cm, a body weight of 11.1±2.10g, and a mouth gap of 0.91±0.12cm were caught. A mandarin fish with a body length of 6cm ate 2 loaches with a body length of 4cm, and a mandarin fish with a body length of 7cm ate 3 loaches with a body length of 4cm;

[0045] (3) Feed training method

[0046] 3.1 Preparation of domesticated bait fish

[0047] On August 31, 2023, the mandarin fish entered the cages and were starved for 2 days before being fed with fresh loach fry for 3 days. VC astaxanthin was sprayed on the fish just after entering the cages, 30g / mu, to strengthen the physique of the mandarin fish and enhance its stress resistance. The domestication procedure began after the mandarin fish showed obvious clustering phenomenon. Loach was used as live bait fish for mandarin fish domestication, and the mandarin fish was fed at a fixed time and place every day. A 5-meter-long fry was placed every 10m at the entrance of the pond dedicated to raising loaches. m of ground traps, and collect the loaches in the traps at 5 o'clock the next day; to domesticate 100,000 mandarin fish with a body length of 6.44±0.59cm, 300kg of loaches with a body length of 2.9±0.25cm must be captured, of which 250kg are placed in a -20℃ freezer, and chilled bait is prepared in advance, and the remaining 50kg is placed in an indoor temporary holding pond with an aerator at the bottom. The above bait fish are all waiting to be used as food for the domesticated mandarin fish.

[0048] 3.2 Feed processing

[0049] The mandarin fish feed uses Jie Da mandarin fish special feed, mandarin fish No. 1 feed, crude protein 46%, particle size 2.0mm, length 1.5-2.0cm, mandarin fish No. 2 feed, crude protein 52%, particle size 2.5mm, length 1.5-2cm; before each feeding, the following treatment is performed: soak in water for 7 minutes, dry for 3 minutes, soak in water again, and perform vacuum treatment.

[0050] 3.3 Domestication Procedure

[0051] From September 1 to September 22, 2023, the domestication of mandarin fish went through 6 stages: starvation → live loach → fresh dead loach coated with powder → fresh dead loach coated with powder + chilled loach coated with powder → chilled loach coated with powder + feed → feed. The food for mandarin fish involved includes: live bait, chilled bait, dead bait coated with powder, chilled bait coated with powder and feed. The chilled bait is loach seedlings frozen in a -20℃ refrigerator in advance. The loach coated with powder makes the mandarin fish familiar with the taste of the feed in advance and promotes the mandarin fish to eat the feed.

[0052] Phase 1: Feed Acclimation Day 1

[0053] Mandarin fish fry were starved for 1 day in the cage.

[0054] Stage 2: Day 2 to 4 of feed acclimatization

[0055] 1. Feeding live bait: Feed once at 8:00 am and 17:00 pm every day, feed 0.05kg live loach for every kilogram of mandarin fish; 2. Spray VC astaxanthin in advance; 3. Sieve the seedlings before entering the cage; 4. Check for parasites under a microscope; 5. Disinfect with 0.2mg / L iodine; 6. Check the feeding situation; 7. If the physique is healthy and the feeding is normal, proceed to the next step. The key point of this link is to ensure that the mandarin fish seedlings are healthy and adapt to the cage environment;

[0056] Stage 3: Feed acclimatization day 5 to 9

[0057] 1. Fresh dead baits are coated with powder and fed once at 8:00 am and 17:00 pm every day. 0.05 kg of dead loach coated with powder is fed to each kilogram of mandarin fish. 2. Cultivate the rhythm of feeding: turn on the water flow stimulation for 10 minutes before feeding, and the water flow speed is 0.8 m / s; 3. Microscopically inspect parasites and monitor water quality every day. Use VC astaxanthin in special weather; 4. Focus: Screen and eliminate fry that do not eat dead bait, strengthen rhythm, promote clustering for feeding, and prevent diseases;

[0058] Stage 4: Feed acclimatization day 10-12

[0059] 1. Feed fresh dead bait wrapped in powder + ice-fresh bait wrapped in powder (ratio 1:1), feed 0.03kg of dead bait wrapped in powder + 0.03kg of ice-fresh bait wrapped in powder per kilogram of mandarin fish; 2. Cultivate rhythm: feed 10 minutes after the water flow signal stimulation, time point: form a cluster to grab food phenomenon; 3. Microscopically inspect parasites daily, monitor water quality, and use VC astaxanthin in special weather; 4. Observe that 90% of the mandarin fish fry are full, sieve and grade them for the next step of domestication, with the focus on strengthening rhythm, grabbing food state, and familiarizing with the shape and smell of feed;

[0060] Stage 5: Feed acclimatization 13-15 days

[0061] 1. Chilled loach powder + feed: Feed chilled loach powder and feed once in the morning and evening. The feeding base of each kilogram of mandarin fish starts with 0.06kg of chilled loach powder. On the 13th, 14th and 15th days, the proportion of pellet feed is increased by 20%, 50% and 80%; 2. Cultivate rhythm: Feed after 10 minutes of running water stimulation, and the time point is to form a cluster feeding phenomenon; 3. Microscopically inspect parasites and monitor water quality every day. Use VC astaxanthin in special weather; 4. Observe that 90% of mandarin fish fry are full and eat, and the focus is to strengthen the rhythm and form a feeding state;

[0062] Stage 6: Feed acclimatization day 16 to 21

[0063] 1. Feeding: increase the feed amount by 0.01kg per kilogram of mandarin fish per day; 2. Cultivation rhythm: feed 10 minutes after water stimulation, time point: form a cluster to grab food phenomenon; 3. Microscopically inspect parasites and monitor water quality every day, use VC astaxanthin in special weather; 4. Only when the satiety rate of feed intake reaches more than 85%, can the finished feed mandarin fish be cultivated, and the focus is on strengthening and stabilizing feed feeding to prevent the phenomenon of returning to the mouth, and prepare for the finished feed mandarin fish.

[0064] (4) Domestication results

[0065] 4.1 Statistics on the domestication rate of mandarin fish

[0066] The feed acclimation technology for mandarin fish fry using loach as the re-export bait took a total of 21 days. The initial body length of the fry was 6.44±0.59cm, and the body weight was 11.1±2.10g, with a total of 20,000 fry. At the end of the acclimation, a body length of 9.71±0.33cm and a body weight of 17.88±0.71g were obtained, with a total of 17,000 mandarin fish fry that stably fed the feed, and the acclimation rate was 85%.

[0067] 4.2 Statistics of domesticated growth of mandarin fish

[0068] The weight of feed fed to the cages was recorded daily. At the end of feeding, samples were taken in time to observe the feeding situation of the mandarin fish and calculate the feeding rate of the mandarin fish in each cage. The mandarin fish that did not eat feed were screened out every 5 days and put into separate cages for re-acclimation. The body length, mouth crack, body height, body width, intestinal length, and body weight of the mandarin fish fry hatched in the same batch in the feed acclimation group and the mandarin fish fry grown under natural conditions were measured for 1 week and 3 weeks after acclimation. The results showed that the mandarin fish adapted to the feed grew fast, and the feeding of feed made up for the shortcomings of insufficient bait fish, slow growth, and weak disease resistance of mandarin fish in the natural state. At the same time, artificial feeding of mandarin fish through this acclimation process is an effective method to promote the growth and development of mandarin fish. After 1 week and 3 weeks of acclimation, the morphological differences of mandarin fish in the easy-to-acclimate group and the natural environment group were measured, as shown in Table 1 and Table 2:

[0069] Table 1 Effects of 1-week acclimation on the morphology of Siniperca chuatsi

[0070]

[0071] Table 2 Effects of 3-week acclimation on the morphology of Siniperca chuatsi

[0072]

[0073] The weight differences of the internal organs and empty shells of the mandarin fish in the diet acclimation group for 3 weeks and the mandarin fish in the natural environment were measured, see Table 3:

[0074] Table 3 The difference in weight of internal organs and empty shells between the acclimated group and the natural environment

[0075]

[0076] As shown in Table 3, the acclimated group of mandarin fish after 3 weeks of feed acclimation showed significant differences in multiple internal organs and empty shell weights compared to mandarin fish grown in a natural environment. This reflects that the acclimated group of mandarin fish has a better growth rate and physical condition under artificial breeding conditions, and can more effectively utilize feed resources and promote weight gain. At the same time, the digestive system of the acclimated group of mandarin fish is more developed, which can more effectively digest and absorb the nutrients in the feed, thereby supporting its faster growth rate.

[0077] (5) Management of finished feed mandarin fish breeding

[0078] Mandarin fish that have been domesticated for 3 weeks can be fed using a feed feeding machine to save manpower; regularly check whether there is enough feed in the feed machine; test the ammonia nitrogen content, dissolved oxygen, pH value and total water hardness in the water every other day; examine a mandarin fish in poor condition under a microscope every day, make gill slices for microscopic observation of parasites, and fill in the electronic breeding log on time.

[0079] Example 2

[0080] The artificial feeding of mandarin fish was carried out according to the model of Example 1. The volume of the net cage used in the feeding process was 12m 3 This example investigates the effect of feeding density of mandarin fish cages on the feeding habits of mandarin fish. The results are shown in Table 4.

[0081] Table 4 Effect of stocking density on the feeding habits of Siniperca chuatsi

[0082]

[0083] As shown in Table 4, the cage density has a certain effect on the feeding domestication of mandarin fish. When the feeding density is 278 fish / m3, the feeding domestication rate of mandarin fish reaches 85% after 3 weeks of feeding; when the density is 55.5 fish / m3, the feeding domestication rate is 78%. The higher the density, the better the feeding domestication effect of mandarin fish.

[0084] Table 5 shows the domestication and germination of mandarin fish in this embodiment.

[0085]

[0086] As shown in Table 5, after 3 weeks of domestication, the domestication success rate reached 85%. After domestication, 85,000 of the 100,000 mandarin fish fingerlings in the experiment were able to completely switch to artificial feed, with a body weight of 17.98±0.71g and a body length of 9.91±0.33cm.

[0087] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for domesticating mandarin fish using large-scale loach as transit bait, characterized in that: The domestication method comprises the following steps: S1. On the first day of acclimation, the mandarin fish fry were starved; S2. On the 2nd to 4th day of acclimation, live bait is fed: live loaches are fed once in the morning and evening every day, and 0.05 kg is fed for every kilogram of mandarin fish. The seedlings are screened, microscopically examined for parasites, disinfected, and checked for feeding conditions. The seedlings with healthy physique and normal feeding are used for the next step; S3, Transit feeding: S3.1 On the 5th to 9th day of acclimation, feed fresh dead loaches coated with powder once in the morning and evening, 0.05kg per kilogram of mandarin fish, cultivate feeding rhythm, examine parasites under a microscope, monitor water quality, and make sure that 90% of the mandarin fish fry are full, and eliminate those that do not eat the dead bait; S3.2 On the 10th to 12th day of domestication, feed fresh dead loach powder and ice fresh loach powder once in the morning and evening, 0.06 kg per kilogram of mandarin fish, cultivate feeding rhythm, microscopically examine parasites, monitor water quality, and when the mandarin fish fry are 90% full, sieve and grade them for the next step of domestication; S3.3 On the 13th to 15th day of acclimation, feed fresh loach powder and feed once in the morning and evening. On the 13th, 14th and 15th days, the proportion of pellet feed is increased by 20%, 50% and 80% to cultivate feeding rhythm, conduct microscopic examination for parasites, monitor water quality, and ensure that 90% of the mandarin fish fry are full; S3.4 On the 16th to 21st day of domestication, feed is given and the feeding amount is increased by 0.01kg of feed per kilogram of mandarin fish per day; feeding rhythm is cultivated, parasites are examined under a microscope, water quality is monitored, and when the satiety rate of feed intake reaches more than 85%, the finished feed mandarin fish can be cultivated.

2. The method for domesticating mandarin fish using large-scale loach as transit bait according to claim 1, characterized in that: In step S3.2, the ratio of fresh dead loach coated with flour to chilled fresh loach coated with flour is 1:

1.

3. The method for domesticating mandarin fish using large-scale loach as re-export bait according to claim 1, characterized in that: Before the domestication, the relevant facilities are disinfected, a feeding platform is built to provide shade, and a feeding attractant light device is installed in the breeding cage.

4. The method for domesticating mandarin fish using large-scale loach as re-export bait according to claim 1, characterized in that: The mandarin fish fry used for domestication in step S1 are pre-roughened.

5. The method for domesticating mandarin fish using large-scale loach as re-export bait according to claim 4, characterized in that: The rough specification is 6±0.5cm.

6. The method for domesticating mandarin fish using large-scale loach as re-export bait according to claim 1, characterized in that: The loach described in step S2 is Paraloach sphaerocephalus, whose unique long strip shape is highly similar to the shape of the feed.

7. The method for domesticating mandarin fish using large-scale loach as re-export bait according to claim 1, characterized in that: The powder coating of fresh dead loach refers to mixing dead loach with mandarin fish feed, wherein the mandarin fish feed is a special feed for Jie Da mandarin fish, mandarin fish feed No. 2, with 52% crude protein, 2.5mm particle size and 1.5-2cm length.

8. The method for domesticating mandarin fish using large-scale loach as transit bait according to claim 1, characterized in that: Anti-stress treatment is carried out during the domestication process.

9. The method for domesticating mandarin fish using large-scale loach as transit bait according to claim 1, characterized in that: The specific method of cultivating the feeding rhythm is to feed the rats after 10 minutes of water flow stimulation.

10. The method for domesticating mandarin fish using large-scale loach as transit bait according to claim 9, characterized in that: The water flow rate is 0.8 m / s, the nozzle is 30 cm away from the water surface, and the water flow contacts the water surface at an angle of 45° during operation.

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