Camellia oil-based diglyceride for weight loss and its preparation method

Abstract

This invention relates to the field of chemical preparation technology, and in particular to a camellia oil-based diglyceride for weight loss and its preparation method. The invention involves passing a raw material sample through a funnel at several preset temperatures, recording the time taken for the sample to pass through the funnel, calculating parameters characterizing the effect of temperature, conducting a glycerolysis reaction in the raw material to obtain a reaction solution, determining whether the reaction temperature needs adjustment, stirring the reaction solution at preset time intervals to obtain the stirring force value, and obtaining the color value of the reaction solution at preset time intervals, calculating parameters characterizing the reaction progress, and determining whether the reaction is complete. The product in the reaction solution is purified by molecular distillation to obtain camellia oil-based diglyceride. This invention improves the preparation efficiency of camellia oil-based diglyceride by adjusting the reaction temperature based on the influence of temperature on the flowability of the raw material and judging the reaction progress by obtaining changes in the viscosity and color value of the reaction solution.

Description

Technical Field

[0001] This invention relates to the field of chemical preparation technology, and in particular to a camellia oil-based diglyceride for weight loss and its preparation method. Background Technology

[0002] Camellia oil, extracted from the seeds of the camellia tree, has been found to have extremely high nutritional value. It is a unique edible oil in my country with a production history spanning thousands of years and can be used to prepare diglycerides. The preparation of diglycerides mostly uses animal and vegetable oils or glycerol and fatty acids as raw materials, and processes such as hydrolysis, esterification, transesterification, acid hydrolysis, and alcoholysis to produce products with different DAG contents. There are three main methods for preparing DAG: hydrolysis, glycerol hydrolysis, and esterification. Currently, glycerol hydrolysis is the most important method for preparing DAG. It uses vegetable oil and glycerol as substrates, and an enzymatic catalytic glycerol hydrolysis reaction yields a reaction product mainly composed of metaglycerides. After separation to remove monoglycerides and free fatty acids, a DAG-rich oil is obtained.

[0003] Chinese Patent Publication No. CN112322670A discloses a method for synthesizing diglycerides, comprising the following steps: (1) mixing oil, lipase, and solvent, and carrying out a hydrolysis reaction to obtain a hydrolysate; (2) under the action of diglyceride lipase, esterifying the hydrolysate obtained in step (1) with glycerol; wherein the lipase in step (1) is lipase MAS1 or MAS1-H108A. This invention's synthesis method can prepare high-purity diglycerides, and is simple to operate and low in cost.

[0004] However, the following problems still exist in the existing technology:

[0005] In practice, glycerolysis reactions involve a large amount of substrates such as glycerol, resulting in a high viscosity of the reaction system. This high viscosity can lead to reduced mass transfer efficiency, limiting effective contact between the substrate and the enzyme, and ultimately causing low reaction efficiency. Summary of the Invention

[0006] Therefore, the present invention provides a camellia oil-based diglyceride for weight loss and its preparation method, in order to overcome the problem that in the prior art, the glycerolysis reaction has a large amount of substrate such as glycerol, resulting in a high viscosity of the reaction system. The high viscosity of the system may lead to a decrease in mass transfer efficiency, which limits the effective contact between the substrate and the enzyme, resulting in a low reaction efficiency.

[0007] To achieve the above objectives, the present invention provides a method for preparing camellia oil-based diglycerides for weight loss.

[0008] Step S1: Extract raw material samples from the raw materials, pass the raw material samples through a funnel at several preset temperatures, and record the time it takes for the raw material samples to pass through the funnel at each preset temperature.

[0009] Step S2: Obtain the average time of the raw material sample passing through the funnel at each preset temperature and the fluctuation value of the time of the raw material sample passing through the funnel at each preset temperature, and calculate the temperature influence characterization parameter based on the average time and the fluctuation value of the time.

[0010] Step S3: Perform glycerolysis reaction in the raw materials to obtain a reaction solution. Determine whether to adjust the reaction temperature of the glycerolysis reaction based on the temperature influence characterization parameter, and adjust the reaction temperature of the glycerolysis reaction based on the temperature influence characterization parameter.

[0011] Step S4: Stir the reaction solution during the glycerol hydrolysis reaction at preset time intervals, obtain the stirring force value, and obtain the color value of the reaction solution at preset time intervals. Calculate the reaction progress characterization parameters by obtaining the change value of the stirring force value and the change value of the color value.

[0012] Step S5: Determine whether the reaction is complete based on the reaction progress characterization parameters;

[0013] Step S6: After the reaction is completed, the product in the reaction solution is purified by molecular distillation to obtain camellia oil-based diglyceride.

[0014] Further, in step S1, the process of obtaining the average time of the raw material sample passing through the funnel at each preset temperature and the fluctuation value of the time of the raw material sample passing through the funnel at each preset temperature includes,

[0015] The time it takes for a raw material sample to pass through a funnel at each preset temperature is obtained;

[0016] The average time is calculated based on the time it takes for the raw material sample to pass through the funnel at each preset temperature.

[0017] The fluctuation value of the time taken for the raw material sample to pass through the funnel at each preset temperature is calculated according to formula (1).

[0018]

[0019] In formula (1), tb represents the fluctuation value of time, ti represents the time of the i-th preset temperature, te represents the average time, n represents the number of preset temperatures, and n is an integer greater than 1.

[0020] Further, in step S2, the temperature influence characterization parameters are calculated according to formula (2).

[0021]

[0022] In formula (2), E represents the temperature influence parameter, tb0 represents the preset time fluctuation threshold, and te0 represents the preset average time threshold.

[0023] Furthermore, in step S3, the process of determining whether to adjust the reaction temperature of the glycerol hydrolysis reaction based on the temperature influence characterization parameter includes:

[0024] The temperature effect characterization parameters are compared with the preset temperature effect characterization parameter comparison threshold.

[0025] If the temperature influence characterization parameter is greater than the preset temperature influence characterization parameter comparison threshold, then it is determined that the reaction temperature of the glycerol hydrolysis reaction should be adjusted.

[0026] Furthermore, in step S3, the reaction temperature of the glycerol hydrolysis reaction is adjusted according to the temperature influence characterization parameter, wherein the temperature influence characterization parameter is positively correlated with the reaction temperature of the glycerol hydrolysis reaction.

[0027] Furthermore, in step S3, the glycerol hydrolysis reaction in the raw material includes adding lipase to the raw material, wherein the lipase is immobilized on a carrier.

[0028] Furthermore, in step S4, the process of obtaining the change value of the stirring force and the change value of the colorimetric value includes,

[0029] The stirring force value and color value are obtained at preset time intervals;

[0030] The difference between two consecutive stirring force values ​​is the change in stirring force value, and the difference between two consecutive chromaticity values ​​is the change in chromaticity value.

[0031] Further, in step S4, the reaction process characterization parameters are calculated according to formula (3).

[0032]

[0033] In formula (3), D represents the reaction process characterization parameter, S represents the color value change value, S0 represents the preset color value change value threshold, F represents the change value of stirring force, and F0 represents the preset change value of stirring force.

[0034] Further, in step S5, the process of determining whether the reaction is complete based on the reaction progress characterization parameters includes,

[0035] The reaction process characterization parameters are compared with the preset reaction process characterization parameter comparison thresholds;

[0036] If the reaction process characterization parameter is less than the preset reaction process characterization parameter comparison threshold, the reaction is determined to be complete.

[0037] Furthermore, a camellia oil-based diglyceride for weight loss is prepared according to the above-described method for preparing camellia oil-based diglyceride for weight loss.

[0038] Compared with existing technologies, this invention involves passing raw material samples through a funnel at several preset temperatures, recording the time it takes for the raw material samples to pass through the funnel, calculating the temperature-related parameters, conducting a glycerolysis reaction in the raw materials to obtain a reaction solution, determining whether the reaction temperature of the glycerolysis reaction needs to be adjusted, stirring the reaction solution during the glycerolysis reaction at preset time intervals, obtaining the stirring force value, and obtaining the color value of the reaction solution at preset time intervals, calculating the reaction progress parameters, determining whether the reaction is complete, and purifying the product in the reaction solution through molecular distillation to obtain camellia oil-based diglyceride. This invention improves the preparation efficiency of camellia oil-based diglyceride by adjusting the reaction temperature based on the influence of temperature on the flowability of the raw materials and judging the reaction progress by obtaining changes in the viscosity and color value of the reaction solution.

[0039] In particular, this invention obtains the average time of raw material samples passing through the funnel at each preset temperature and the fluctuation value of the time of raw material samples passing through the funnel at each preset temperature. The fluidity of the raw material is an important factor affecting the reaction efficiency. Obtaining the fluidity of the raw material and the change of the fluidity of the raw material with temperature can provide data support for subsequent processes and improve the efficiency of camellia oil-based diglyceride preparation.

[0040] In particular, the present invention determines whether to adjust the reaction temperature of the glycerolysis reaction based on the temperature influence characterization parameter. When the fluidity of the raw material is poor and the temperature has a significant impact on the fluidity, the temperature influence characterization parameter is large. Since the poor fluidity of the raw material will lead to low reaction efficiency, when the temperature has a significant impact on the fluidity, the fluidity of the raw material can be adjusted to a certain extent by adjusting the reaction temperature, thereby improving the preparation efficiency of camellia oil-based diglyceride.

[0041] In particular, the present invention determines whether the reaction is complete based on the reaction progress characterization parameters. Since the components in the reaction solution change continuously during the reaction process, the color value and viscosity of the reaction solution change with the changes in the components in the reaction solution. By obtaining the force value of the reaction solution being stirred, the viscosity of the reaction solution can be characterized to a certain extent. The change value of the stirring force value and the change value of the color value can be characterized to a certain extent. If the change value of the stirring force value and the change value of the color value are small, the reaction can be determined to be complete. By using the reaction progress characterization parameters, the reaction progress can be judged more reliably, thereby improving the preparation efficiency of camellia oil-based diglyceride while ensuring sufficient reaction. Attached Figure Description

[0042] Figure 1 This is a step diagram illustrating the preparation method of camellia oil-based diglyceride for weight loss according to an embodiment of the invention;

[0043] Figure 2 This is a logic diagram for determining whether to adjust the reaction temperature of the glycerol hydrolysis reaction based on temperature influence characterization parameters, as shown in an embodiment of the invention.

[0044] Figure 3 This is a logic diagram for determining whether a reaction is complete based on reaction process characterization parameters, as an embodiment of the invention.

[0045] Figure 4 A schematic diagram of a raw material sample passing through a funnel, as shown in an embodiment of the invention. Detailed Implementation

[0046] To make the objectives and advantages of the present invention clearer, the present invention will be further described below with reference to embodiments; it should be understood that the specific embodiments described herein are merely for explaining the present invention and are not intended to limit the present invention.

[0047] Preferred embodiments of the present invention will now be described with reference to the accompanying drawings. Those skilled in the art should understand that these embodiments are merely illustrative of the technical principles of the present invention and are not intended to limit the scope of protection of the present invention.

[0048] It should be noted that in the description of this invention, the terms "upper", "lower", "left", "right", "inner", "outer", etc., which indicate directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings. This is only for the convenience of description and is not intended to indicate or imply that the device or element must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation of this invention.

[0049] Furthermore, it should be noted that, in the description of this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0050] Please see Figures 1-4 As shown, Figure 1 This is a flowchart illustrating the steps of a method for preparing camellia oil-based diglyceride for weight loss, as described in an embodiment of the invention. Figure 2 This is a logic diagram illustrating how to determine whether to adjust the reaction temperature of the glycerol hydrolysis reaction based on temperature-related characterization parameters, as described in an embodiment of the invention. Figure 3 This is a logic diagram for determining whether a reaction is complete based on reaction process characterization parameters, as described in an embodiment of the invention. Figure 4 This is a schematic diagram of a raw material sample passing through a funnel, illustrating the method for preparing camellia oil-based diglyceride for weight loss according to the present invention, which includes:

[0051] Step S1: Extract raw material samples from the raw materials, pass the raw material samples through a funnel at several preset temperatures, and record the time it takes for the raw material samples to pass through the funnel at each preset temperature.

[0052] Step S2: Obtain the average time of the raw material sample passing through the funnel at each preset temperature and the fluctuation value of the time of the raw material sample passing through the funnel at each preset temperature, and calculate the temperature influence characterization parameter based on the average time and the fluctuation value of the time.

[0053] Step S3: Perform glycerolysis reaction in the raw materials to obtain a reaction solution. Determine whether to adjust the reaction temperature of the glycerolysis reaction based on the temperature influence characterization parameter, and adjust the reaction temperature of the glycerolysis reaction based on the temperature influence characterization parameter.

[0054] Step S4: Stir the reaction solution during the glycerol hydrolysis reaction at preset time intervals, obtain the stirring force value, and obtain the color value of the reaction solution at preset time intervals. Calculate the reaction progress characterization parameters by obtaining the change value of the stirring force value and the change value of the color value.

[0055] Step S5: Determine whether the reaction is complete based on the reaction progress characterization parameters;

[0056] Step S6: After the reaction is completed, the product in the reaction solution is purified by molecular distillation to obtain camellia oil-based diglyceride.

[0057] In this embodiment, the raw material is camellia oil.

[0058] like Figure 2 As shown, in this embodiment, 20ml of camellia oil was selected as a camellia oil sample. The camellia oil sample was poured into a funnel, and the time it took for all 20ml of camellia oil to pass through the funnel into the beaker was recorded.

[0059] In this embodiment, the preset temperature is a temperature range selected based on the optimal temperature of lipase. Optionally, the preset temperature is 36℃, 38℃, 40℃, 42℃, or 44℃.

[0060] In this embodiment, a force sensor is provided on the stirrer used to stir the reaction dissolution, which can acquire the force value during the stirring process and record the average force value during the stirring process as the stirring force value.

[0061] In this embodiment, an image of the reaction solution is acquired using an image acquisition device, and the colorimetric value of the reaction solution is obtained using image analysis software. Optionally, the image acquisition device can be an industrial CCD camera, and the image analysis software can be MATLAB.

[0062] Specifically, in step S1, the process of obtaining the average time of the raw material sample passing through the funnel at each preset temperature and the fluctuation value of the time of the raw material sample passing through the funnel at each preset temperature includes,

[0063] The time it takes for a raw material sample to pass through a funnel at each preset temperature is obtained;

[0064] The average time is calculated based on the time it takes for the raw material sample to pass through the funnel at each preset temperature.

[0065] The fluctuation value of the time taken for the raw material sample to pass through the funnel at each preset temperature is calculated according to formula (1).

[0066]

[0067] In formula (1), tb represents the fluctuation value of time, ti represents the time of the i-th preset temperature, te represents the average time, n represents the number of preset temperatures, and n is an integer greater than 1.

[0068] Specifically, this invention obtains the average time of raw material samples passing through the funnel at each preset temperature and the fluctuation value of the time of raw material samples passing through the funnel at each preset temperature. The fluidity of the raw material is an important factor affecting the reaction efficiency. Obtaining the fluidity of the raw material and the change of the fluidity of the raw material with temperature can provide data support for subsequent processes and improve the efficiency of camellia oil-based diglyceride preparation.

[0069] Specifically, in step S2, the temperature influence characterization parameters are calculated according to formula (2).

[0070]

[0071] In formula (2), E represents the temperature influence parameter, tb0 represents the preset time fluctuation threshold, and te0 represents the preset average time threshold.

[0072] In this embodiment, several camellia oils are tested in advance to obtain the time fluctuation value tbc and the average time tec of the camellia oil passing through the funnel at several preset temperatures. Let tb0 = a × tbc, where a is the time fluctuation value coefficient, 0.9 < a ​​< 1.1, and let te0 = b × tec, where b is the average time coefficient, 0.9 < b < 1.1.

[0073] Specifically, in step S3, the process of determining whether to adjust the reaction temperature of the glycerol hydrolysis reaction based on the temperature influence characterization parameters includes:

[0074] The temperature effect characterization parameters are compared with the preset temperature effect characterization parameter comparison threshold.

[0075] If the temperature influence characterization parameter is greater than the preset temperature influence characterization parameter comparison threshold, then it is determined that the reaction temperature of the glycerol hydrolysis reaction should be adjusted.

[0076] In this embodiment, the preset threshold E0 for comparing the temperature influence characterization parameters is selected within the range of [1.55, 1.60].

[0077] Specifically, the present invention determines whether to adjust the reaction temperature of the glycerolysis reaction based on the temperature influence characterization parameter. When the fluidity of the raw material is poor and the temperature has a significant impact on the fluidity, the temperature influence characterization parameter is large. Since the poor fluidity of the raw material will lead to low reaction efficiency, when the temperature has a significant impact on the fluidity, the fluidity of the raw material can be adjusted to a certain extent by adjusting the reaction temperature, thereby improving the preparation efficiency of camellia oil-based diglyceride.

[0078] Specifically, in step S3, the reaction temperature of the glycerol hydrolysis reaction is adjusted according to the temperature influence characterization parameter, wherein the temperature influence characterization parameter is positively correlated with the reaction temperature of the glycerol hydrolysis reaction.

[0079] In this embodiment, optionally,

[0080] E0 < E < 1.62, the reaction temperature of the glycerol hydrolysis reaction is T1 = T0 + ΔT1;

[0081] 1.62≤E, the reaction temperature of the glycerol hydrolysis reaction is T2=T0+△T2;

[0082] T0 is the initial reaction temperature of the glycerol hydrolysis reaction, which is the optimal temperature for lipase. In this embodiment, T0 is 40℃, ΔT1 is the first reaction temperature adjustment amount, ΔT2 is the second reaction temperature adjustment amount, and 0℃ < ΔT1 < ΔT2 < 2℃.

[0083] Specifically, in step S3, the glycerol hydrolysis reaction in the raw material includes adding lipase to the raw material, wherein the lipase is immobilized on a carrier.

[0084] In this embodiment, the carrier for lipase immobilization is activated carbon.

[0085] Specifically, in step S4, the process of obtaining the change value of the stirring force and the change value of the colorimetric value includes,

[0086] The stirring force value and color value are obtained at preset time intervals;

[0087] The difference between two consecutive stirring force values ​​is the change in stirring force value, and the difference between two consecutive chromaticity values ​​is the change in chromaticity value.

[0088] Specifically, in step S4, the reaction process characterization parameters are calculated according to formula (3).

[0089]

[0090] In formula (3), D represents the reaction process characterization parameter, S represents the color value change value, S0 represents the preset color value change value threshold, F represents the change value of stirring force, and F0 represents the preset change value of stirring force.

[0091] In this embodiment, the stirring force F1 and the color value S1 of the reaction solution during the initial stirring of the glycerol hydrolysis reaction are obtained. Let F0 = c × F1, where c is the force coefficient, 0.01 < c < 0.05. Let S0 = d × S1, where d is the color value coefficient, 0.01 < d < 0.05.

[0092] Specifically, in step S5, the process of determining whether the reaction is complete based on the reaction progress characterization parameters includes:

[0093] The reaction process characterization parameters are compared with the preset reaction process characterization parameter comparison thresholds;

[0094] If the reaction process characterization parameter is less than the preset reaction process characterization parameter comparison threshold, the reaction is determined to be complete.

[0095] In this embodiment, the preset threshold for comparing reaction process characterization parameters is selected within the range of [1.52, 1.55].

[0096] Specifically, this invention determines whether the reaction is complete based on the reaction progress characterization parameters. Since the components in the reaction solution change continuously during the reaction process, the color value and viscosity of the reaction solution change with the changes in the components in the reaction solution. By obtaining the force value of the reaction solution being stirred, the viscosity of the reaction solution can be characterized to a certain extent. The change value of the stirring force value and the change value of the color value can be characterized to a certain extent. If the change value of the stirring force value and the change value of the color value are small, the reaction can be determined to be complete. By using the reaction progress characterization parameters, the reaction progress can be judged more reliably, thereby improving the preparation efficiency of camellia oil-based diglyceride while ensuring sufficient reaction.

[0097] Specifically, a camellia oil-based diglyceride for weight loss is prepared according to the above-mentioned method for preparing camellia oil-based diglyceride for weight loss.

[0098] The technical solution of the present invention has been described above with reference to the preferred embodiments shown in the accompanying drawings. However, it will be readily understood by those skilled in the art that the scope of protection of the present invention is obviously not limited to these specific embodiments. Without departing from the principles of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will all fall within the scope of protection of the present invention.

Claims

1. A method for preparing camellia oil-based diglyceride for weight loss, characterized in that, include: Step S1: Extract raw material samples from the raw materials, pass the raw material samples through a funnel at several preset temperatures, and record the time it takes for the raw material samples to pass through the funnel at each preset temperature. Step S2: Obtain the average time of the raw material sample passing through the funnel at each preset temperature and the fluctuation value of the time of the raw material sample passing through the funnel at each preset temperature, and calculate the temperature influence characterization parameter based on the average time and the fluctuation value of the time. Step S3: Perform glycerolysis reaction in the raw materials to obtain a reaction solution. Determine whether to adjust the reaction temperature of the glycerolysis reaction based on the temperature influence characterization parameter, and adjust the reaction temperature of the glycerolysis reaction based on the temperature influence characterization parameter. Step S4: Stir the reaction solution during the glycerol hydrolysis reaction at preset time intervals, obtain the stirring force value, and obtain the color value of the reaction solution at preset time intervals. Calculate the reaction progress characterization parameters by obtaining the change value of the stirring force value and the change value of the color value. Step S5: Determine whether the reaction is complete based on the reaction progress characterization parameters; Step S6: After the reaction is completed, the product in the reaction solution is purified by molecular distillation to obtain camellia oil-based diglyceride. In step S1, the process of obtaining the average time of the raw material sample passing through the funnel at each preset temperature and the fluctuation value of the time of the raw material sample passing through the funnel at each preset temperature includes, The time it takes for a raw material sample to pass through a funnel at each preset temperature is obtained; The average time is calculated based on the time it takes for the raw material sample to pass through the funnel at each preset temperature. The fluctuation value of the time taken for the raw material sample to pass through the funnel at each preset temperature is calculated according to formula (1). （1） In formula (1), tb represents the fluctuation value of time, ti represents the time of the i-th preset temperature, te represents the average time, n represents the number of preset temperatures, and n is an integer greater than 1. In step S2, the parameters characterizing the effect of temperature are calculated according to formula (2). （2） In formula (2), E represents the temperature influence characterization parameter, tb0 represents the preset time fluctuation threshold, and te0 represents the preset average time threshold. In step S3, the reaction temperature of the glycerol hydrolysis reaction is adjusted according to the temperature influence characterization parameter, wherein the temperature influence characterization parameter is positively correlated with the reaction temperature of the glycerol hydrolysis reaction. If E0 < E < 1.62, the reaction temperature of the glycerol hydrolysis reaction is T1 = T0 + ΔT1; If 1.62≤E, the reaction temperature of the glycerol hydrolysis reaction is T2=T0+ΔT2; If T0 is the initial reaction temperature of the glycerol hydrolysis reaction, ΔT1 is the first reaction temperature adjustment amount, ΔT2 is the second reaction temperature adjustment amount, and 0℃ < ΔT1 < ΔT2 < 2℃; In step S4, the reaction process characterization parameters are calculated according to formula (3). （3） In formula (3), D represents the reaction process characterization parameter, S represents the color value change value, S0 represents the preset color value change value threshold, F represents the change value of stirring force, and F0 represents the preset change value of stirring force. In step S5, the process of determining whether the reaction is complete based on the reaction progress characterization parameters includes... The reaction process characterization parameters are compared with the preset reaction process characterization parameter comparison thresholds; If the reaction process characterization parameter is less than the preset reaction process characterization parameter comparison threshold, the reaction is determined to be complete. The preset threshold values ​​for comparing reaction process characterization parameters are selected within the range of [1.52, 1.55].

2. The method for preparing camellia oil-based diglyceride for weight loss according to claim 1, characterized in that, In step S3, the process of determining whether to adjust the reaction temperature of the glycerol hydrolysis reaction based on the temperature influence characterization parameters includes... The temperature effect characterization parameters are compared with the preset temperature effect characterization parameter comparison threshold. If the temperature influence characterization parameter is greater than the preset temperature influence characterization parameter comparison threshold, then it is determined that the reaction temperature of the glycerol hydrolysis reaction should be adjusted. The preset temperature influence characterization parameter comparison threshold E0 is selected in the range of [1.55, 1.60].

3. The method for preparing camellia oil-based diglyceride for weight loss according to claim 1, characterized in that, In step S3, the glycerol hydrolysis reaction is carried out in the raw material, including adding lipase to the raw material, wherein the lipase is immobilized on a carrier.

4. The method for preparing camellia oil-based diglyceride for weight loss according to claim 3, characterized in that, In step S4, the process of obtaining the change values ​​of the stirring force and colorimetric values ​​includes: The stirring force value and color value are obtained at preset time intervals; The difference between two consecutive stirring force values ​​is the change in stirring force value, and the difference between two consecutive chromaticity values ​​is the change in chromaticity value.

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