A yogurt fungal count detection device and detection method based on quantitative sampling

Abstract

The present invention relates to the technical field of yogurt fungal detection, and specifically relates to a yogurt fungal quantity detection device and detection method based on quantitative sampling. The device includes a sterile box, a turntable and a container arranged inside the sterile box. A moving mechanism is arranged on the top of the sterile box, and a monitoring component, a first suction and release component and a second suction and release component are arranged on the moving mechanism. Through the first suction and release component and the second suction and release component arranged on the moving mechanism of the present invention, they can respectively perform the operations of sucking and releasing the diluent and recovering the excess diluent. This design reduces the intervention of manual operation through automatic control, improves the accuracy of the entire detection process, realizes the automatic and precise dispensing of the diluent, thereby improving the accuracy and reliability of yogurt fungal detection. The monitoring component is used to monitor the dripping amount of the diluent in real time to ensure that the released diluent is equal to or exceeds the preset amount, ensuring the reliability and consistency of the fungal detection results.

Description

Technical Field

[0001] The present invention relates to the technical field of yogurt fungus detection, and particularly to a yogurt fungus quantity detection device and detection method based on quantitative sampling. Background Art

[0002] In the production process of yogurt, the detection of fungus content is an important step to ensure quality. In the prior art, the commonly used agar plate counting method requires a series of operations such as sampling, dilution, and coating to determine the fungus content in the sample. However, this method has some defects. For example, when using a spreader to coat the agar plate, the fungus is likely to adhere to the spreader, resulting in a reduction in the actual number of fungi in the sample, thus affecting the accuracy of the detection result. In addition, the manual operation of the spreader is also likely to cause uneven distribution of the dilution solution, and the colonies may be mixed together, affecting the subsequent fungus counting.

[0003] Chinese Patent Application CN118302512A discloses a yogurt fungus quantity detection device and detection method. This device includes components such as a sterile box, a sterilization lamp, a centrifugal component, and a blowing component. A sterilization lamp is provided inside the sterile box to create a sterile environment; the centrifugal component is fixed in the middle position of the sterile box, and the agar plate is located above the centrifugal component; the blowing component is movably arranged on the top of the sterile box. The whole device spreads the yogurt dilution solution evenly from the center of the agar plate to the outside through the centrifugal component, and the blowing component further helps the uniform distribution of the dilution solution through a blower. This patent uses centrifugal force through the centrifugal component to evenly spread the yogurt dilution solution on the agar plate and blows it through the blower to ensure that the dilution solution can quickly cover the entire plate, thus avoiding the aggregation of the dilution solution. The release process of the dilution solution is controlled by a valve, and a cleaning device is also provided inside the device to reduce contamination. However, this patent has the following defects: 1. Dilution solution drying problem: During the process of the blower blowing the dilution solution, it is easy to accelerate its drying, which may affect the generation of colonies and lead to inaccurate detection; 2. Uneven thickness of the dilution solution: The valve controls the release amount of the dilution solution, and it is difficult to accurately control the thickness of the dilution solution released each time, resulting in inconsistent distribution of the dilution solution on the plate. Summary of the Invention

[0004] In view of the above problems, a yogurt fungus quantity detection device and detection method based on quantitative sampling are provided. In the present invention, a first liquid suction and release component and a second liquid suction and release component are arranged on a moving mechanism, which can respectively perform the operations of sucking and releasing the dilution solution and recovering the excess dilution solution. This design reduces the intervention of manual operation through automatic control, improves the accuracy of the entire detection process, realizes the automatic and precise dosing of the dilution solution, thereby improving the accuracy and reliability of yogurt fungus detection. By monitoring the component to monitor the dropping amount of the dilution solution in real time, it is ensured that the released dilution solution is equal to or exceeds the preset amount to ensure the reliability and consistency of the fungus detection result.

[0005] To solve the problems of the existing technology, the present invention provides a yogurt fungal count detection device based on quantitative sampling, which includes a sterile box, a turntable and a plurality of containers arranged inside the sterile box. The turntable is used to drive the uniform spreading of the diluent, and the containers are used to hold the diluent. A moving mechanism capable of moving between the turntable and the diluent containers is arranged on the inner wall of the top of the sterile box. A monitoring component for monitoring the dropping amount of the diluent, a first suction and release component for sucking the diluent in the container and releasing it on the turntable, and a second suction and release component for sucking the excess diluent released on the turntable are arranged on the moving mechanism; the first suction and release component and the second suction and release component are respectively located at both ends of the moving mechanism, and the monitoring component is located between the first suction and release component and the second suction and release component.

[0006] Preferably, a driving box is arranged on the moving mechanism. A support shaft extending along the height direction of the sterile box is arranged inside the driving box. A support frame is arranged on the support shaft. The structures of the first suction and release component and the second suction and release component are completely the same. The first suction and release component and the second suction and release component are arranged at both ends of the support frame in a mirror-symmetrical state. Both the first suction and release component and the second suction and release component include a straw for sucking and releasing the diluent, an air pump for driving the straw to operate, and a support plate parallel to the axis of the support shaft. Two parallel connecting rods hinged to it are arranged at the top and bottom of the support plate. The ends of the two connecting rods far from the support plate are hinged to the support frame. A driving component for driving the movement of the support plate is arranged inside the driving box. The air pump is arranged inside the driving box. The straw is a telescopic structure. The straw is connected to the air pump and sleeved on the support plate.

[0007] Preferably, the support frame is rotatably arranged on the support shaft. A first gear is arranged on the top of the support frame. A second gear meshed with the first gear is arranged inside the driving box. A first rotary drive motor for driving its rotation is arranged on the second gear. The driving component is located on one side inside the driving box, and the driving component can only drive the support plate of one of the first suction and release component and the second suction and release component to move.

[0008] Preferably, a wing plate extending in the horizontal direction is arranged on the top of the support frame. A first slide rail is arranged on the wing plate. The driving component includes a telescopic rod arranged between the support plate and the wing plate. A first slider slidably matched with the first slide rail is arranged at the top of the telescopic rod. A spring is arranged between the first slider and the support frame. A driving block capable of sliding along the direction of the first slide rail is arranged inside the driving box. An avoidance groove for avoiding the wing plate is arranged on the driving block.

[0009] Preferably, the driving assembly includes a first lead screw and a second rotary driving motor. The first lead screw is horizontally arranged and rotatably disposed in the driving box. The driving block is sleeved on the first lead screw and is in threaded cooperation with it. The second rotary driving motor is located at one end of the first lead screw. The output shaft of the second rotary driving motor is coaxially arranged with the axis of the first lead screw, and the first lead screw is in transmission connection with the output shaft of the second rotary driving motor. A second slide rail extending along the axis of the first lead screw is arranged in the driving box, and the driving block is slidably disposed on the second slide rail.

[0010] Preferably, a cleaning box filled with cleaning liquid is arranged between the container and the turntable.

[0011] Preferably, the monitoring assembly includes a vision camera, and the vision camera is arranged inside the support shaft and the support frame.

[0012] Preferably, the moving mechanism includes a second lead screw and a third slide rail arranged on the top of the sterile box. The second lead screw is horizontally arranged and rotatably disposed on the top of the sterile box. The third slide rail extends along the axis of the second lead screw. A connection block in threaded cooperation with the second lead screw is arranged on the top of the driving box, and the connection block can slide on the third slide rail.

[0013] Preferably, an annular baffle is arranged on the periphery of the turntable, and the annular baffle is used to prevent the dilution liquid from overflowing.

[0014] A method for detecting the number of fungi in yogurt is applied to the above-mentioned yogurt fungi number detection device based on quantitative sampling, and includes the following steps:

[0015] S1. Place the yogurt sample to be detected in the container in the sterile box, ensure that the environment in the container remains sterile, and ensure that the turntable is in an idle state.

[0016] S2a. Start the moving mechanism, control the first suction and release assembly to move above the container, suck the dilution liquid from the container through the straw of the first suction and release assembly, and drive the first suction and release assembly to move above the turntable.

[0017] S2b. The moving mechanism drives the first suction and release assembly to move from above the container to above the turntable. Above the turntable, release the dilution liquid onto the turntable through the first suction and release assembly. The monitoring assembly continues to monitor the amount of the released dilution liquid to ensure that the dilution liquid is uniform and meets the predetermined amount. If the monitoring assembly detects that the released dilution liquid exceeds the preset amount, control the second suction and release assembly to start, and suck and recover the excess dilution liquid through the straw to ensure that the amount of the dilution liquid on the turntable reaches the preset value; if the dilution liquid does not reach the preset amount, the first suction and release assembly continues to drip the dilution liquid until it reaches the preset value.

[0018] S3. After the diluent is evenly distributed, the turntable stops rotating and the detection process begins. Through incubation for a predetermined period of time, the monitoring component or other subsequent detection equipment starts to detect and analyze the number of colonies on the turntable.

[0019] S4. Record the detection results. By taking and analyzing the colony images formed on the turntable through the monitoring component, transmit the detection data to a computing device for subsequent analysis to obtain a quantitative result of the number of fungi in the yogurt.

[0020] The beneficial effects of the present invention compared with the prior art are as follows:

[0021] 1. In the present invention, through the first suction and release component and the second suction and release component provided on the moving mechanism, they can respectively perform the operations of sucking and releasing the diluent and recovering the excess diluent. This design reduces the intervention of manual operation through automatic control, improves the accuracy of the entire detection process, realizes the automatic and precise dispensing of the diluent, thereby improving the accuracy and reliability of yogurt fungi detection. By the monitoring component, the dripping amount of the diluent is monitored in real time to ensure that the released diluent is equal to or exceeds the preset amount. During the detection process, when the monitoring component finds that the amount of diluent is lower than the preset value, the first suction and release component will continue to release the diluent until the requirement is met; if the amount of diluent is excessive, the second suction and release component is activated to recover the excess diluent. This method of automatic monitoring and adjustment greatly improves the accuracy of diluent dispensing and ensures the reliability and consistency of the fungi detection results.

[0022] 2. In the present invention, the flexible switching of the first suction and release component and the second suction and release component is realized through the rotating structure of the support frame. This rotating design not only improves the operation efficiency of the device, but also ensures that the components can quickly locate to the appropriate positions for sucking and releasing operations. Through the drive system cooperating with the first gear and the second gear, the movement of the support frame is more precise, reducing errors and unnecessary time waste during the operation process. The setting of the sensor further improves the positioning accuracy, enabling the device to automatically adjust the position of the components during diluent distribution and avoiding mistakes caused by human intervention.

[0023] 3. In the present invention, through the setting of the cleaning box, after each use, the suction pipes of the first suction and release component and the second suction and release component can be fully cleaned, avoiding cross-contamination of the diluent between different experiments. This design further ensures the sterility of the device operation and the accuracy of the experimental results. At the same time, the configuration of the visual camera enables real-time monitoring of the dripping and distribution of the diluent. The visual camera uses image recognition technology to detect the state of the diluent and feeds the data back to the control system, thereby achieving higher-precision control. Description of the Drawings

[0024] Figure 1It is a schematic cross-sectional structure diagram of a yogurt fungal quantity detection device based on quantitative sampling.

[0025] Figure 2 It is a schematic three-dimensional cross-sectional structure diagram of a yogurt fungal quantity detection device based on quantitative sampling.

[0026] Figure 3 It is a schematic three-dimensional structure diagram of a moving mechanism and a turntable in a yogurt fungal quantity detection device based on quantitative sampling.

[0027] Figure 4 It is a schematic three-dimensional structure diagram of a first suction and release component, a second suction and release component and a turntable in a yogurt fungal quantity detection device based on quantitative sampling.

[0028] Figure 5 It is a schematic cross-sectional structure diagram of a first suction and release component, a second suction and release component and a turntable in a yogurt fungal quantity detection device based on quantitative sampling.

[0029] Figure 6 It is a schematic three-dimensional structure diagram of a first suction and release component and a second suction and release component in a yogurt fungal quantity detection device based on quantitative sampling Figure 1 .

[0030] Figure 7 It is a schematic three-dimensional structure diagram of a first suction and release component and a second suction and release component in a yogurt fungal quantity detection device based on quantitative sampling Figure 2 .

[0031] Figure 8 It is a schematic three-dimensional structure diagram inside a drive box in a yogurt fungal quantity detection device based on quantitative sampling.

[0032] Figure 9 It is a schematic three-dimensional structure diagram of a drive component and a support frame in a yogurt fungal quantity detection device based on quantitative sampling.

[0033] Figure 10 It is a schematic three-dimensional structure diagram of a support frame in a yogurt fungal quantity detection device based on quantitative sampling.

[0034] Figure 11 It is a schematic three-dimensional structure diagram of a support frame during rotation in a yogurt fungal quantity detection device based on quantitative sampling.

[0035] The reference numerals in the figure are:

[0036] 1 - Sterile box; 11 - Turntable; 111 - Baffle; 12 - Container; 13 - Cleaning box; 2 - Moving mechanism; 21 - First suction and release component; 211 - Straw; 212 - Air pump; 213 - Support plate; 2131 - Connecting rod; 22 - Second suction and release component; 23 - Monitoring component; 231 - Visual camera; 24 - Driving box; 241 - Support shaft; 242 - Support frame; 2421 - First gear; 2422 - Wing plate; 24221 - First slide rail; 243 - Second gear; 2431 - First rotary drive motor; 244 - Driving component; 2441 - Telescopic rod; 24411 - First slider; 24412 - Spring; 2442 - Driving block; 24421 - Avoidance groove; 24422 - Second slide rail; 2443 - First lead screw; 24431 - Second rotary drive motor; 245 - Second lead screw; 246 - Third slide rail; 247 - Connecting block. Detailed implementation mode

[0037] To further understand the features, technical means, specific purposes, and functions achieved by the present invention, the present invention will be described in further detail below in conjunction with the accompanying drawings and specific implementation modes.

[0038] As Figures 1 to 5 and Figure 8 shown: A yogurt fungal count detection device based on quantitative sampling includes a sterile box 1, a turntable 11 and a plurality of containers 12 arranged inside the sterile box 1. The turntable 11 is used to drive the uniform spreading of the diluent, and the container 12 is used to hold the diluent. A moving mechanism 2 capable of moving between the turntable 11 and the diluent container 12 is arranged on the inner top wall of the sterile box 1. A monitoring component 23 for monitoring the dropping amount of the diluent, a first suction and release component 21 for sucking the diluent in the container 12 and releasing it on the turntable 11, and a second suction and release component 22 for sucking the excess diluent released on the turntable 11 are arranged on the moving mechanism 2; the first suction and release component 21 and the second suction and release component 22 are respectively located at both ends of the moving mechanism 2, and the monitoring component 23 is located between the first suction and release component 21 and the second suction and release component 22.

[0039] When it is necessary to detect the diluent, the moving mechanism 2 moves between the diluent container 12 and the turntable 11. The movement of the moving mechanism 2 drives the first liquid suction and discharge assembly 21 and the second liquid suction and discharge assembly 22 provided on the moving mechanism 2. The moving mechanism 2 first drives the first liquid suction and discharge assembly 21 to move above the container 12, and the first liquid suction and discharge assembly 21 extracts the diluent. Then, the moving mechanism 2 drives it to move above the turntable 11 for release. At this time, the monitoring assembly 23 monitors the release amount of the diluent to ensure that when the turntable 11 rotates to evenly spread the diluent, the diluent can be evenly distributed on the turntable 11. It should be noted that the second liquid suction and discharge assembly 22 is in an idle state at this time. The monitoring assembly 23 ensures that the dripping amount of the diluent of the first liquid suction and discharge assembly 21 is equal to or greater than the preset amount by monitoring it in real time. When the diluent is less than the preset value, the first liquid suction and discharge assembly 21 continues to release until it reaches the preset value; when the diluent exceeds the preset value, the second liquid suction and discharge assembly 22 is started, and the second liquid suction and discharge assembly 22 extracts the excess diluent to ensure the balance of the diluent amount on the turntable 11 and prevent it from affecting the subsequent detection of the fungal quantity. By setting the monitoring assembly 23, the dripping amount of the diluent can be monitored in real time to ensure that the diluent amount released each time is accurate, avoiding affecting the accuracy of the fungal detection due to insufficient or excessive diluent amount. By this method, the purpose of quantitatively controlling the diluent is achieved, thereby improving the consistency and reliability of the detection result of the fungal quantity in yogurt. The first liquid suction and discharge assembly 21 and the second liquid suction and discharge assembly 22 operate in the sterile box 1 through automatic control, reducing the chance of manual contact with the diluent, effectively reducing the risk of external contamination, ensuring the sterility of the entire detection process, and improving the reliability and precision of the detection. The setting of the second liquid suction and discharge assembly 22 can detect and timely suck the excess diluent when the diluent is released onto the turntable 11, avoiding the excessive diluent from affecting the even distribution of the fungi on the turntable 11. This design can automatically compensate for the dripping amount of the diluent and further improve the accuracy of the fungal detection.

[0040] Through the settings of the container 12, the first liquid suction and discharge assembly 21, and the second liquid suction and discharge assembly 22, compared with the prior art method of releasing liquid through a valve, it is more difficult to achieve quantitative delivery in this way, and the diluent is manually proportioned. Secondly, the liquid remaining at the valve port may cause interference problems during multiple detections. By directly placing the container 12 to be detected in the sterile box 1, the cleanliness of the diluent can be improved, which helps to improve the accuracy of the fungal quantity detection. At the same time, the first liquid suction and discharge assembly 21 can also proportion the yogurt and the diluent, so as to obtain a more accurate diluent and further improve the detection accuracy.

[0041] Such as Figures 4 to 11As shown in the figure: A driving box 24 is provided on the moving mechanism 2. A support shaft 241 extending in the height direction of the sterile box 1 is provided in the driving box 24. A support frame 242 is provided on the support shaft 241. The structures of the first suction and release assembly 21 and the second suction and release assembly 22 are exactly the same. The first suction and release assembly 21 and the second suction and release assembly 22 are arranged at both ends of the support frame 242 in a mirror-symmetrical state. Both the first suction and release assembly 21 and the second suction and release assembly 22 include a straw 211 for sucking and releasing the diluent, an air pump 212 for driving the straw 211 to operate, and a support plate 213 parallel to the axis of the support shaft 241. Two parallel connecting rods 2131 hinged to it are provided at the top and bottom of the support plate 213. One end of the two connecting rods 2131 far from the support plate 213 is hinged to the support frame 242. A driving component 244 for driving the movement of the support plate 213 is provided in the driving box 24. The air pump 212 is provided in the driving box 24. The straw 211 is a telescopic structure. The straw 211 is connected to the air pump 212. The straw 211 is sleeved on the support plate 213.

[0042] By starting the driving component 244, the movement of the support plate 213 can be driven. Since the support plate 213 is hinged to the support frame 242 through parallel connecting rods 2131, it can be ensured that the support plate 213 always remains parallel during the up and down movement. This design reduces the inclination or instability during the movement process, ensures the stable operation of the straw 211 when sucking and releasing the diluent, and enables the first suction and release assembly 21 and the second suction and release assembly 22 to be located above the turntable 11 during the movement process. Thus, when the diluent in the straw 211 moves to the turntable 11, it will not contact any components. At the same time, it can adjust its position on the turntable 11 and the distance between the straw 211 and the turntable 11 through movement. Therefore, it can improve the control effect of the quantitative delivery of the diluent and improve the accuracy of the detection process. The air pump 212 is provided in the driving box 24 and is connected to the straw 211 in a sleeved manner. This compact design helps to save the internal space of the device, simplifies the structural layout, and improves the working efficiency of the entire device. The straw 211 adopts a telescopic structure and can be automatically adjusted according to the different heights of the diluent container 12 or the agar plate, improving the adaptability of the device.

[0043] It should be noted that a fixed mounting head for operating the diluent is provided at the end of the straw 211. The mounting head is made of hard material and will not deform. The straw 211 is used to connect the mounting head and the air pump 212, so the straw 211 is a telescopic structure.

[0044] Such as Figures 4 to 11As shown in the figure: The support frame 242 is rotatably arranged on the support shaft 241. A first gear 2421 is arranged at the top of the support frame 242. A second gear 243 meshing with the first gear 2421 is arranged in the drive box 24. A first rotary drive motor 2431 for driving its rotation is arranged on the second gear 243. The drive assembly 244 is located on one side inside the drive box 24. The drive assembly 244 can only drive the support plate 213 of one of the first suction and release assembly 21 and the second suction and release assembly 22 to move.

[0045] Since the drive assembly 244 can only drive the support plate 213 of one of the first suction and release assembly 21 and the second suction and release assembly 22 to move, this method enables the first suction and release assembly 21 and the second suction and release assembly 22 to be independently controlled, so that the two alternately operate on the diluent dropped on the turntable 11, thereby improving the flexibility of the operation, reducing possible interaction interference, ensuring the accurate suction and release of the diluent. By arranging the support frame 242 rotatably on the support shaft 241 and cooperating with the drive structure of the first gear 2421 and the second gear 243, the support frame 242 can rotate flexibly, quickly aligning the first suction and release assembly 21 or the second suction and release assembly 22 that needs to be operated with the diluent container 12 or the turntable 11. This method helps to more quickly distribute the diluent or recover the excess diluent, improving the efficiency of the entire operation process. The second gear 243 is driven to rotate by the first rotary drive motor 2431. The rotation of the second gear 243 drives the rotation of the first gear 2421 meshing with it. The rotation of the first gear 2421 drives the rotation of the support frame 242. In order to make the entire moving mechanism 2 highly automated, sensors can be arranged on the support frame 242. Through the arrangement of the sensors, the movement positions of the first suction and release assembly 21 and the second suction and release assembly 22 can be accurately positioned. The automated precise movement reduces the need for human intervention, improves the consistency and repeatability of the operation, and helps to improve the accuracy of the detection results.

[0046] As Figures 3 to 11 shown in the figure: A wing plate 2422 extending horizontally in its direction is arranged at the top of the support frame 242. A first slide rail 24221 is arranged on the wing plate 2422. The drive assembly 244 includes a telescopic rod 2441 arranged between the support plate 213 and the wing plate 2422. A first slider 24411 slidably matched with the first slide rail 24221 is arranged at the top of the telescopic rod 2441. A spring 24412 is arranged between the first slider 24411 and the support frame 242. A drive block 2442 capable of sliding along the direction of the first slide rail 24221 is arranged in the drive box 24. An avoidance groove 24421 for avoiding the wing plate 2422 is arranged on the drive block 2442.

[0047] In the initial state, due to the elastic force of the spring 24412, the telescopic rod 2441 keeps a fixed distance between the first slider 24411 of the telescopic rod 2441 and the support frame 242. At this time, the telescopic rod 2441 is in a contracted state, and the two connecting rods 2131 are close to the horizontal state. At this time, the support plate 213 drives the suction pipe 211 to be located above the turntable 11. At this time, the device is in a contracted state, which is convenient for the moving mechanism 2 to drive the first suction and release assembly 21 and the second suction and release assembly 22 to move. When it is necessary to switch between the first suction and release assembly 21 and the second suction and release assembly 22, the first rotary drive motor 2431 drives the second gear 243 to rotate. The rotation of the second gear 243 drives the rotation of the first gear 2421. The rotation of the first gear 2421 drives the support frame 242 to rotate 180 degrees. The rotation of the support frame 242 can drive the rotation of the wing plate 2422. Thus, the support frame 242 can drive the first suction and release assembly 21 or the second suction and release assembly 22 to move to one side of the drive assembly 244. It should be noted that at this time, the drive block 2442 is located at the edge of the drive box 24, so that the wing plate 2422 will not be interfered by the drive block 2442 during the rotation process. When the positions of the first suction and release assembly 21 and the second suction and release assembly 22 are interchanged, the suction pipe 211 will be stretched, and when the positions of the two are reset, the suction pipe 211 will recover by contraction; after the movement of the support frame 242 is completed, the drive block 2442 is started. The drive block 2442 can slide along the direction of the first slide rail 24221. The drive block 2442 avoids the interference of the wing plate 2422 through the avoidance groove 24421, so that the drive block 2442 can abut against the first slider 24411 on the wing plate 2422, and gradually drives the first slider 24411 to move along the first slide rail 24221 towards the support frame 242. Since the movement of the first slider 24411 drives the movement of the telescopic rod 2441, the telescopic rod 2441 drives the movement of the support plate 213 through its own movement. Since there are two connecting rods 2131 on the support plate 213, at this time, the support plate 213 moves downward and towards the center side of the drive box 24. The telescopic rod 2441 extends to match the distance between its support frames 242, thereby driving the first suction and release assembly 21 or the second suction and release assembly 22 to operate, so that the first suction and release assembly 21 or the second suction and release assembly 22 can approach the turntable 11 or the container 12 to ensure the accurate suction and release of the diluent. After the drive block 2442 is reset, the first slider 24411 can drive the telescopic rod 2441 to contract under the action of the elastic reset of the spring 24412, so that the connecting rod 2131 drives the support plate 213 to move upward and away from the turntable 11 or the container 12.

[0048] Such as Figures 3 to 11As shown: The driving component 244 includes a first lead screw 2443 and a second rotary drive motor 24431. The first lead screw 2443 is horizontally and rotatably arranged in the drive box 24. The drive block 2442 is sleeved on the first lead screw 2443 and is in threaded cooperation with it. The second rotary drive motor 24431 is located at one end of the first lead screw 2443. The output shaft of the second rotary drive motor 24431 is coaxially arranged with the axis of the first lead screw 2443, and the first lead screw 2443 is drivingly connected to the output shaft of the second rotary drive motor 24431. A second slide rail 24422 extending along the axis of the first lead screw 2443 is arranged in the drive box 24. The drive block 2442 is slidably arranged on the second slide rail 24422.

[0049] By starting the second rotary drive motor 24431, the rotation of the output shaft of the second rotary drive motor 24431 drives the rotation of the first lead screw 2443 drivingly connected to it. The rotation of the first lead screw 2443 drives the movement of the drive block 2442 in threaded cooperation with it, so that the drive block 2442 can slide along the second slide rail 24422. Thus, the drive block 2442 can drive the movement of the first slider 24411, and then drive the movement of the telescopic rod 2441 through the first slider 24411. The movement of the telescopic rod 2441 drives the movement of the connecting rod 2131, thereby driving the movement of the support plate 213 of the first suction and release component 21 or the second suction and release component 22. The movement of the support plate 213 drives the movement of the suction pipe 211, so that the first suction and release component 21 or the second suction and release component 22 can approach or move away from the turntable 11 or the container 12, thus improving the flexibility of the operation, reducing possible interaction interference, and ensuring the accurate suction and release of the diluent.

[0050] Through the lead screw drive method, the device can control the first suction and release component 21 or the second suction and release component 22 more stably and precisely.

[0051] As Figure 1 and Figure 2 shown: A cleaning box 13 filled with cleaning liquid is arranged between the container 12 and the turntable 11.

[0052] Through the arrangement of the cleaning box 13, the suction pipe 211 of the first suction and release component 21 or the second suction and release component 22 after releasing the diluent can suck the cleaning liquid in the cleaning box 13, and then clean the inside of the suction pipe 211. Thus, it can be ensured that the first suction and release component 21 or the second suction and release component 22 will not contaminate the sample when extracting the subsequent diluent, thereby improving the detection accuracy of the device.

[0053] As Figures 2 to 8As shown: The monitoring component 23 includes a visual camera 231, and the visual camera 231 is arranged inside the support shaft 241 and the support frame 242.

[0054] Through the setting of the visual camera 231, the amount and distribution of the diluent dropped onto the turntable 11 can be monitored in real time, ensuring that the diluent can be accurately and evenly spread on the turntable 11. The visual camera 231 can detect the dropping amount and distribution state of the diluent through image recognition technology, and feed the data back to the control system, and then adjust the operation of the suction and release component according to the detection results to achieve a more precise control effect. This not only improves the accuracy of detection, but also reduces the possible errors in the operation process.

[0055] In order to further ensure the accuracy of the dropping of the diluent, a pressure sensor can also be arranged on the turntable 11 to measure the diluent dropped onto the turntable 11, which can cooperate with the visual camera 231 to improve the accuracy of the recognition of the diluent.

[0056] As Figures 1 to 5 shown: The moving mechanism 2 includes a second lead screw 245 and a third slide rail 246 arranged on the top of the sterile box 1. The second lead screw 245 is horizontally and rotatably arranged on the top of the sterile box 1, and the third slide rail 246 extends along the axis direction of the second lead screw 245. A connecting block 247 that is threadedly engaged with the second lead screw 245 is arranged on the top of the driving box 24, and the connecting block 247 can slide on the third slide rail 246.

[0057] By the rotation of the second lead screw 245, the movement of the connecting block 247 threadedly engaged with it is driven. By the connecting block 247 moving smoothly along the third slide rail 246, the driving box 24 can move horizontally along the top of the sterile box 1. The driving of the second lead screw 245 can be realized by a servo motor, thereby accurately controlling the position of the driving box 24, realizing the precise positioning and movement of the first suction and release component 21 or the second suction and release component 22 in the sterile box 1, and thus improving the operation flexibility of the device and the accuracy of the diluent distribution.

[0058] As Figures 1 to 5 shown: An annular baffle 111 is arranged on the periphery of the turntable 11, and the annular baffle 111 is used to block the overflow of the diluent.

[0059] Through the setting of the annular baffle 111, it is used to block the overflow of the diluent due to the centrifugal force when the turntable 11 rotates at a high speed. This setting can effectively prevent the diluent from scattering or splashing outside the turntable 11 during the rotation process, ensure that the diluent is evenly distributed on the surface of the turntable 11, guarantee the accuracy of the fungal quantity detection and the cleanliness of the operation environment. The annular baffle 111 not only improves the controllability of the experimental process, but also reduces the waste of the diluent and the burden of the cleaning work.

[0060] As Figures 1 to 5 and Figure 8 shown: A method for detecting the number of fungi in yogurt, which is applied to the above-mentioned yogurt fungi number detection device based on quantitative sampling, includes the following steps:

[0061] S1. Place the yogurt sample to be detected in container 12 in sterile box 1, ensure that the environment in container 12 remains sterile, and ensure that turntable 11 is in an idle state.

[0062] S2a. Start moving mechanism 2, control the first suction and release component 21 to move above container 12, suck the diluent from container 12 through the straw 211 of the first suction and release component 21, and drive the first suction and release component 21 to move above turntable 11.

[0063] S2b. Moving mechanism 2 drives the first suction and release component 21 to move from above container 12 to above turntable 11. Above turntable 11, release the diluent onto turntable 11 through the first suction and release component 21. Monitoring component 23 continues to monitor the amount of diluent released to ensure that the diluent is uniform and meets the predetermined amount. If monitoring component 23 detects that the released diluent exceeds the preset amount, control the second suction and release component 22 to start, and suck and recover the excess diluent through the straw 211 to ensure that the amount of diluent on turntable 11 reaches the preset value; if the diluent does not reach the preset amount, the first suction and release component 21 continues to drip the diluent until it reaches the preset value.

[0064] S3. After the diluent is evenly distributed, turntable 11 stops rotating, and the detection process starts. Through cultivation for a predetermined time, monitoring component 23 or other subsequent detection devices start to detect and analyze the number of colonies on turntable 11.

[0065] S4. Record the detection results, take and analyze the colony images formed on turntable 11 through monitoring component 23, and transmit the detection data to a computing device for subsequent analysis to obtain the quantitative result of the number of fungi in the yogurt.

[0066] The embodiments only express one or several implementation manners of the present invention, and their descriptions are relatively specific and detailed, but should not be construed as a limitation on the scope of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several deformations and improvements can still be made, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims

1. A yogurt fungus quantity detection device based on quantitative sampling, comprising a sterile box (1) and a turntable (11) and a plurality of containers (12) arranged inside the sterile box (1), wherein the turntable (11) is used to drive a diluent to be evenly spread, and the container (12) is used to contain the diluent. A moving mechanism (2) capable of moving between the turntable (11) and the diluent container (12) is arranged on the top inner wall of the sterile box (1), characterized in that: The moving mechanism (2) is provided with a monitoring component (23) for monitoring the amount of diluent dripped in, a first suction and release component (21) for sucking the diluent in the container (12) and releasing it on the rotating disk (11), and a second suction and release component (22) for sucking excess diluent released on the rotating disk (11); The first suction and placement component (21) and the second suction and placement component (22) are respectively located at two ends of the moving mechanism (2), and the monitoring component (23) is located between the first suction and placement component (21) and the second suction and placement component (22); The moving mechanism (2) is provided with a driving box (24), a supporting shaft (241) extending in the height direction of the sterile box (1) is provided in the driving box (24), a supporting frame (242) is provided on the supporting shaft (241), the first suction and placement assembly (21) and the second suction and placement assembly (22) are completely identical in structure, and the first suction and placement assembly (21) and the second suction and placement assembly (22) are provided at both ends of the supporting frame (242) in a mirror-symmetrical state; The monitoring component (23) comprises a visual camera (231), and the visual camera (231) is arranged inside the support shaft (241) and the support frame (242); If the amount of diluent dripped in monitored by the visual camera (231) exceeds a preset value, the second suction and placement component (22) is controlled to work to absorb the excess diluent released on the turntable (11) until the amount of diluent on the turntable (11) reaches a preset value; if the amount of diluent dripped in monitored by the visual camera (231) is less than the preset value, the first suction and placement component (21) is controlled to work to continue releasing the diluent on the turntable (11) until the amount of diluent on the turntable (11) reaches the preset value; The first suction and placement assembly (21) and the second suction and placement assembly (22) both comprise a suction pipe (211) for suctioning and placing a diluent, an air pump (212) for driving the suction pipe (211) to operate, and a support plate (213) parallel to the axis of the support shaft (241). A driving assembly (244) for driving the support plate (213) to move is arranged in the driving box (24). The driving assembly (244) can only drive the support plate (213) of one of the first suction and placement assembly (21) and the second suction and placement assembly (22) to move.

2. A yogurt fungus quantity detection device based on quantitative sampling according to claim 1, characterized in that: The top and bottom of the support plate (213) are both provided with two connecting rods (2131) which are parallel to each other and hinged thereto; one end of the two connecting rods (2131) which is away from the support plate (213) is hinged to the support frame (242); the air pump (212) is arranged in the drive box (24); the suction pipe (211) is a telescopic structure; the suction pipe (211) is connected to the air pump (212); and the suction pipe (211) is sleeved on the support plate (213).

3. A yogurt fungus quantity detection device based on quantitative sampling according to claim 2, characterized in that: The support frame (242) is rotatably arranged on the support shaft (241); a first gear (2421) is arranged on the top of the support frame (242); a second gear (243) meshingly connected with the first gear (2421) is arranged in the drive box (24); a first rotary drive motor (2431) for driving the second gear (243) to rotate is arranged on the second gear (243); and the drive assembly (244) is located on one side of the drive box (24).

4. A yogurt fungus quantity detection device based on quantitative sampling according to claim 3, characterized in that: A wing plate (2422) extending in a horizontal direction is arranged at the top of the support frame (242), and a first slide rail (24221) is arranged on the wing plate (2422). The driving assembly (244) comprises a telescopic rod (2441) arranged between the support plate (213) and the wing plate (2422), and a first slider (24411) slidably matched with the first slide rail (24221) is arranged at the top of the telescopic rod (2441), and a spring (24412) is arranged between the first slider (24411) and the support frame (242). A driving block (2442) capable of sliding along the direction of the first slide rail (24221) is arranged in the driving box (24), and an avoidance groove (24421) for avoiding the wing plate (2422) is arranged on the driving block (2442).

5. A yogurt fungus quantity detection device based on quantitative sampling according to claim 4, characterized in that: The driving assembly (244) comprises a first screw rod (2443) and a second rotation driving motor (24431); the first screw rod (2443) is rotatably arranged in a horizontal state in a driving box (24); the driving block (2442) is sleeved on the first screw rod (2443) and threadedly engaged with the first screw rod; the second rotation driving motor (24431) is located on one end of the first screw rod (2443); the output shaft of the second rotation driving motor (24431) is coaxially arranged with the axis of the first screw rod (2443); the first screw rod (2443) is drivingly connected to the output shaft of the second rotation driving motor (24431); a second slide rail (24422) extending along the axis direction of the first screw rod (2443) is arranged in the driving box (24); the driving block (2442) is slidably arranged on the second slide rail (24422).

6. A yogurt fungus quantity detection device based on quantitative sampling according to claim 2, characterized in that: A cleaning box (13) containing cleaning liquid is arranged between the container (12) and the rotating disk (11).

7. A yogurt fungus quantity detection device based on quantitative sampling according to claim 2, characterized in that: The moving mechanism (2) comprises a second screw rod (245) and a third slide rail (246) arranged at the top of the sterile box (1); the second screw rod (245) is rotatably arranged at the top of the sterile box (1) in a horizontal state; the third slide rail (246) extends along the axial direction of the second screw rod (245); a connecting block (247) threadedly matched with the second screw rod (245) is arranged at the top of the drive box (24); and the connecting block (247) can slide on the third slide rail (246).

8. The yogurt fungus quantity detection device based on quantitative sampling according to claim 1, characterized in that: An annular baffle (111) is provided on the circumference of the rotating disk (11), and the annular baffle (111) is used to prevent the diluent from overflowing.

9. A method for detecting the number of yogurt fungi, applied to a yogurt fungi number detection device based on quantitative sampling as claimed in any one of claims 1 to 8, characterized in that: The following steps are included: S1, placing a yogurt sample to be tested in a container (12) in a sterile box (1), ensuring that the environment in the container (12) remains sterile, and ensuring that the turntable (11) is in an idle state; S2a, starting the moving mechanism (2), controlling the first suction and placement component (21) to move to the top of the container (12), sucking the diluent from the container (12) through the suction pipe (211) of the first suction and placement component (21), and driving the first suction and placement component (21) to move to the top of the turntable (11); S2b, the moving mechanism (2) drives the first suction and placement component (21) to move from above the container (12) to above the turntable (11), and above the turntable (11), the diluent is released onto the turntable (11) by the first suction and placement component (21), and the monitoring component (23) continues to monitor the amount of the released diluent to ensure that the diluent is uniform and meets the predetermined amount. If the monitoring component (23) detects that the released diluent exceeds the preset amount, the second suction and placement component (22) is controlled to start, and the excess diluent is sucked and recovered through the suction pipe (211), so as to ensure that the amount of the diluent on the turntable (11) reaches the preset value; if the diluent does not reach the preset amount, the first suction and placement component (21) continues to drip the diluent until the preset value is reached; S3, after the diluent is evenly distributed, the turntable (11) stops rotating and the detection process begins. After a predetermined period of incubation, the monitoring component (23) or other subsequent detection equipment begins to detect and analyze the number of colonies on the turntable (11); S4, recording the detection results, photographing and analyzing the colony image formed on the turntable (11) through the monitoring component (23), transmitting the detection data to the computing device for subsequent analysis, so as to obtain the quantitative result of the fungus quantity in the yogurt.

Images