An apparatus for collecting greenhouse gases in anaerobic soil culture and its usage method

Abstract

The present invention discloses a greenhouse gas collection device for soil anaerobic cultivation, which relates to the technical field of soil environment, and includes: a screw-mouth glass bottle with a screw-mouth bottle cap; a reducing straight hose joint embedded in the screw-mouth bottle cap, the reducing straight hose joint having a small-diameter end and a large-diameter end that are connected and communicated, and the small-diameter end extending into the screw-mouth glass bottle; an aluminum foil gas collection bag hermetically connected to the large-diameter end. The present invention can create a strict anaerobic environment, ensure the accuracy and reliability of anaerobic experimental conditions, and improve the precision of experimental results. The use of a reducing straight hose joint effectively amplifies the headspace volume, avoids the risk of gas leakage caused by excessive gas pressure, improves the safety of the experiment, and ensures the integrity and effectiveness of experimental data.

Description

Technical Field

[0001] The present invention relates to the technical field of soil environment, and particularly relates to a greenhouse gas collection device for anaerobic cultivation of soil and a method for using the same. Background Art

[0002] Rising temperatures may stimulate soil microbial activity, disrupt nutrient cycling, and ultimately transform soil from a carbon sink to a carbon source, increasing the production of greenhouse gases and exacerbating global warming. As one of the largest carbon pools on Earth, even minor changes in soil organic matter can significantly affect the production of greenhouse gases.

[0003] Soil respiration is a comprehensive manifestation of biological activity and chemical reaction processes in soil. It not only includes the respiration of plant roots but also encompasses the energy-yielding metabolic processes of soil microorganisms. In this complex ecosystem, changes in the metabolic pathways of microorganisms under anaerobic conditions can lead to the production of different types and amounts of greenhouse gases, such as methane, carbon dioxide, nitrous oxide, etc. The production of greenhouse gases is particularly evident in flooded paddy fields, wetlands, and landfill soils. Therefore, it is of great significance to study the production of greenhouse gases in anaerobic soils.

[0004] Traditional greenhouse gas collection devices for anaerobic cultivation of soil have the following limitations:

[0005] 1. A conduit is provided on the bottle cap of the experimental device and sealed with a spring clip. The anaerobic environment created has poor airtightness, and with the pressure changes caused by the production of greenhouse gases, gas leakage easily occurs, seriously affecting the integrity and validity of the data;

[0006] 2. Some experimental devices place the experimental soil in a container, add overlying water for sealing, and use a needle connector to extract gas samples and inject them into a vacuum bag. This method is only applicable to anaerobic conditions in a broad sense and is difficult to create a real and stable anaerobic environment, resulting in relatively low accuracy of experimental results;

[0007] 3. There are also some experimental devices that use a strictly sealed jaw glass bottle to cultivate soil. Although a strict anaerobic condition is constructed, the bottle stopper of the jaw glass bottle can only be used once, causing great waste of resources and making it difficult to achieve long-term and large-scale research use.

[0008] In view of this, how to provide a greenhouse gas collection device for anaerobic cultivation of soil with good airtightness, accurate measurement results, and suitable for long-term and large-scale research use is an urgent problem to be solved by those skilled in the art. Summary of the Invention

[0009] The object of the present invention is to provide a greenhouse gas collection device for soil anaerobic cultivation, so as to solve the problems existing in the prior art, and provide a greenhouse gas collection device for soil anaerobic cultivation with good sealing performance, accurate measurement results, and can be used for long-term and large-scale research.

[0010] To achieve the above object, the present invention provides the following solutions: The present invention provides a screw-mouth glass bottle, and the screw-mouth glass bottle has a screw-mouth bottle cap;

[0011] A reducing straight hose joint, the reducing straight hose joint includes: a disc body, a needle valve hole is opened through the upper and lower surfaces of the screw-mouth bottle cap, the disc body is fixedly arranged in the needle valve hole, and a sealing glue is used for sealing between the disc body and the inner hole wall of the needle valve hole; the inside of the disc body is hollow, the reducing straight hose joint has a small-diameter end and a large-diameter end which are communicated with each other, the large-diameter end and the small-diameter end are respectively arranged on the upper and lower sides of the disc body and both the large-diameter end and the small-diameter end are communicated with the disc body;

[0012] An aluminum foil gas collection bag, the aluminum foil gas collection bag is hermetically communicated with the large-diameter end;

[0013] A rubber inner plug, the rubber inner plug is arranged in the needle valve hole and close to the lower edge of the needle valve hole, a sealing hole is opened through the upper and lower surfaces of the rubber inner plug, the small-diameter end passes through the sealing hole from top to bottom and extends into the screw-mouth glass bottle, and a sealing glue is used for sealing between the small-diameter end and the sealing hole.

[0014] Furthermore, it further includes:

[0015] A rubber tube, one end of the rubber tube is hermetically communicated with the large-diameter end, and the other end is hermetically communicated with the air inlet of the aluminum foil gas collection bag.

[0016] Furthermore, an air inlet valve is arranged at the air inlet of the aluminum foil gas collection bag.

[0017] Furthermore, one end of the rubber tube and the large-diameter end are sealed by vacuum silicone grease, and the other end of the rubber tube and the air inlet of the aluminum foil gas collection bag are sealed by vacuum silicone grease.

[0018] Furthermore, the aperture of the sealing hole is 2 mm.

[0019] The present invention also provides a usage method of a greenhouse gas collection device for soil anaerobic cultivation. Applying the greenhouse gas collection device for soil anaerobic cultivation includes the following steps:

[0020] S1: Assemble the reducing straight hose joint with the aluminum foil gas collection bag and the rubber inner plug respectively, and assemble the screw-mouth bottle cap with the rubber inner plug;

[0021] S2: Add the experimental soil into the screw - mouth glass bottle, rinse the screw - mouth glass bottle with an inert gas until an anaerobic environment is formed inside the screw - mouth glass bottle, and then assemble the screw - mouth bottle cap assembled in S1 to the mouth of the screw - mouth glass bottle.

[0022] S3: Cultivate the experimental soil in the screw - mouth glass bottle for a preset time. Open the intake valve of the aluminum foil gas collection bag, shake the screw - mouth glass bottle, and collect the gas generated inside the screw - mouth glass bottle into the aluminum foil gas collection bag.

[0023] Further, during the soil cultivation process, when it is necessary to introduce a preset experimental gas into the screw - mouth glass bottle, tighten the rubber tube, separate the rubber tube from the intake port of the aluminum foil gas collection bag, connect the other end of the rubber tube to a syringe, suck an inert gas with a volume equal to the volume of the preset experimental gas to be introduced, and then inject the preset experimental gas through the syringe after sucking the inert gas. After injecting the preset experimental gas, connect the other end of the rubber tube to the intake port of the aluminum foil gas collection bag again and seal it.

[0024] Further, when destructive sampling is required, take out the experimental soil in the screw - mouth glass bottle for detection after S3; when it is necessary to continue collecting the gas in the screw - mouth glass bottle, connect another aluminum foil gas collection bag to the reducer straight hose connector, and repeat S2 and S3.

[0025] The present invention discloses the following technical effects:

[0026] 1. It can create a strict anaerobic environment, ensure the accuracy and reliability of anaerobic experimental conditions, and improve the precision of experimental results.

[0027] 2. The use of the reducer straight hose connector effectively amplifies the headspace volume, avoids the risk of gas leakage caused by excessive gas pressure, improves the safety of the experiment, and ensures the integrity and validity of experimental data.

[0028] 3. The gas collection efficiency is high, the operation is simple, it can meet the experimental and research requirements of long - term cultivation and large - scale gas collection, and is conducive to sampling and subsequent analysis.

[0029] 4. The device has a simple structure, low cost and can be reused, with a wide range of applications and good application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0031] Figure 1 Structural schematic diagram of the present invention;

[0032] Figure 2 Schematic diagram of the connection between the screw cap of the present invention and the reducer straight hose joint.

[0033] Among them, 1, screw-threaded glass bottle; 2, rubber inner plug; 3, screw cap; 4, reducer straight hose joint; 5, rubber hose; 6, aluminum foil gas collection bag; 7, experimental soil. Specific implementation manners

[0034] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0035] To make the above objects, features, and advantages of the present invention more obvious and understandable, the present invention will be further described in detail below in conjunction with the accompanying drawings and specific implementation manners.

[0036] Embodiment 1

[0037] As Figure 1 and Figure 2 shown, the embodiment of the present invention provides a greenhouse gas collection device for anaerobic cultivation of soil, including: a screw-threaded glass bottle 1, the screw-threaded glass bottle 1 having a screw cap 3; a reducer straight hose joint 4, the reducer straight hose joint 4 including: a disc main body, the screw cap 3 is provided with a needle valve hole through the upper and lower surfaces, the disc main body is fixedly arranged in the needle valve hole, and the disc main body and the inner hole wall of the needle valve hole are sealed by a sealing glue; the inside of the disc main body is hollow, the reducer straight hose joint 4 has a small-diameter end and a large-diameter end connected to each other, the large-diameter end and the small-diameter end are respectively arranged on the upper and lower sides of the disc main body and both the large-diameter end and the small-diameter end are communicated with the disc main body; an aluminum foil gas collection bag 6, the aluminum foil gas collection bag 6 is hermetically communicated with the large-diameter end; a rubber inner plug 2, the rubber inner plug 2 is arranged in the needle valve hole and close to the lower edge of the needle valve hole, the rubber inner plug 2 is provided with a sealing hole through the upper and lower surfaces, the small-diameter end passes through the sealing hole from top to bottom and extends into the screw-threaded glass bottle 1, and the small-diameter end and the sealing hole are sealed by a sealing glue.

[0038] Both the disc main body and the large-diameter end play a role in buffering gas and expanding the headspace volume, avoiding the risk of gas leakage caused by excessive gas pressure, improving the safety of the experiment, and ensuring the integrity and effectiveness of the experimental data. The aperture of the sealing hole is 2 mm, slightly smaller than the outer diameter of the small-diameter end, and the sealing hole is punched by a puncher. The rubber inner plug 2 is made of butyl rubber.

[0039] In this embodiment, it further includes: a rubber hose 5, one end of the rubber hose 5 is hermetically connected to the large-diameter end, and the other end is hermetically connected to the air inlet of the aluminum foil gas collection bag 6. An air inlet valve is provided at the air inlet of the aluminum foil gas collection bag 6. One end of the rubber hose 5 and the large-diameter end are sealed with vacuum silicone grease, and the other end of the rubber hose 5 and the air inlet of the aluminum foil gas collection bag 6 are sealed with vacuum silicone grease.

[0040] The following describes the usage method of the greenhouse gas collection device for soil anaerobic cultivation in combination with the above embodiments, including the following steps:

[0041] S1: Assemble the variable-diameter straight hose connector 4 with the aluminum foil gas collection bag 6 and the rubber inner plug 2 respectively, and assemble the screw cap 3 with the rubber inner plug 2.

[0042] S2: Add experimental soil 7 to the screw-mouth glass bottle 1, and at the same time add the required drugs, water, etc. for the experiment, and mix the soil with other substances; flush the screw-mouth glass bottle 1 with an inert gas until an anaerobic environment is formed in the screw-mouth glass bottle 1, and assemble the screw cap 3 assembled in S1 to the bottle mouth of the screw-mouth glass bottle 1.

[0043] S3: Cultivate the experimental soil 7 in the screw-mouth glass bottle 1 for a preset time. During the soil cultivation process, it is necessary to introduce a preset experimental gas (isotope gas in this embodiment) into the screw-mouth glass bottle 1. Tighten the rubber hose 5, separate the rubber hose 5 from the air inlet of the aluminum foil gas collection bag 6, connect the other end of the rubber hose 5 to a syringe, use the syringe to suck an inert gas, and the sucked volume is the same as the volume of the preset experimental gas to be introduced. After sucking the inert gas, inject the preset experimental gas through the syringe; after injecting the preset experimental gas, connect the other end of the rubber hose 5 to the air inlet of the aluminum foil gas collection bag 6 again and seal it. Open the air inlet valve of the aluminum foil gas collection bag 6, shake the screw-mouth glass bottle 1, and collect the gas generated in the screw-mouth glass bottle 1 into the aluminum foil gas collection bag 6.

[0044] The gas collected by the aluminum foil gas collection bag 6 is detected by a gas chromatograph (Agilent 7890B) to detect the greenhouse gas concentration; when destructive sampling is required, take out the experimental soil 7 in the screw-mouth glass bottle 1 for detection after S3 ends. When it is necessary to continue collecting the gas in the screw-mouth glass bottle 1, connect another aluminum foil gas collection bag 6 to the variable-diameter straight hose connector 4, and repeat S2 and S3.

[0045] After 90 days of experiment, the collected gas in the experiment can be subjected to machine detection, and the data is accurate and good. The airtightness of the device is good and it can be reused.

[0046] Embodiment 2

[0047] The difference between this embodiment and Embodiment 1 is that each structure is directly assembled. At the same time, an inert gas inlet port and an inert gas outlet port are opened on the screw-mouth glass bottle 1, and electric control valves are provided for both gas ports. After assembly, inert gas is introduced to discharge the original air in the screw-mouth glass bottle 1, and then the electric control valves of the two gas ports are closed. Compared with Embodiment 1, a more stringent anaerobic environment can be created to avoid air residue after inert gas flushing. In some other embodiments, the functions of inert gas inlet and outlet can also be achieved by installing a three-way valve on the screw-mouth glass bottle 1.

[0048] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention.

[0049] The embodiments described above are only for describing the preferred mode of the present invention, and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, various deformations and improvements made by those of ordinary skill in the art to the technical solutions of the present invention shall fall within the protection scope determined by the claims of the present invention.

Claims

1. A greenhouse gas collection device for anaerobic cultivation of soil, characterized in that, Comprising: A screw - mouth glass bottle (1) with a screw - mouth bottle cap (3); A reduced - diameter straight - through hose connector (4), which includes: a disc - shaped main body. The screw - mouth bottle cap (3) is provided with a needle valve hole penetrating through the upper and lower surfaces. The disc - shaped main body is fixedly arranged in the needle valve hole, and the disc - shaped main body and the inner hole wall of the needle valve hole are sealed with sealant; the inside of the disc - shaped main body is hollow. The reduced - diameter straight - through hose connector (4) has a small - diameter end and a large - diameter end that are connected and communicate with each other. The large - diameter end and the small - diameter end are respectively arranged on the upper and lower sides of the disc - shaped main body, and both the large - diameter end and the small - diameter end communicate with the disc - shaped main body; An aluminum - foil gas collection bag (6), which is hermetically connected to the large - diameter end; A rubber inner plug (2), which is arranged in the needle valve hole and close to the lower edge of the needle valve hole. The rubber inner plug (2) is provided with a sealing hole penetrating through the upper and lower surfaces. The small - diameter end passes through the sealing hole from top to bottom and extends into the screw - mouth glass bottle (1), and the small - diameter end and the sealing hole are sealed with sealant; A rubber tube (5), one end of which is hermetically connected to the large - diameter end, and the other end is hermetically connected to the air inlet of the aluminum - foil gas collection bag (6). The air inlet of the aluminum - foil gas collection bag (6) is provided with an air inlet valve.

2. The greenhouse gas collection device for anaerobic soil cultivation according to claim 1, characterized in that, One end of the rubber tube (5) and the large - diameter end are sealed with vacuum grease, and the other end of the rubber tube (5) and the air inlet of the aluminum - foil gas collection bag (6) are sealed with vacuum grease.

3. A method for using a greenhouse gas collection device for anaerobic cultivation of soil, characterized in that, Applying the greenhouse gas collection device for anaerobic cultivation of soil according to claim 2, comprising the following steps: S1: Assemble the reduced - diameter straight - through hose connector (4) with the aluminum - foil gas collection bag (6) and the rubber inner plug (2) respectively, and assemble the screw - mouth bottle cap (3) with the rubber inner plug (2); S2: Add experimental soil (7) into the screw - mouth glass bottle (1), flush the screw - mouth glass bottle (1) with inert gas until an anaerobic environment is formed in the screw - mouth glass bottle (1), and then assemble the screw - mouth bottle cap (3) assembled in S1 to the bottle mouth of the screw - mouth glass bottle (1); S3: Cultivate the experimental soil (7) in the screw - mouth glass bottle (1) for a preset time, open the air inlet valve of the aluminum - foil gas collection bag (6), shake the screw - mouth glass bottle (1), and collect the gas generated in the screw - mouth glass bottle (1) into the aluminum - foil gas collection bag (6).

4. The method of using a greenhouse gas collection device for anaerobic soil cultivation according to claim 3, characterized in that, During the soil cultivation process, when it is necessary to introduce a preset experimental gas into the screw - mouth glass bottle (1), press the rubber tube (5) tightly, separate the rubber tube (5) from the air inlet of the aluminum - foil gas collection bag (6), connect the other end of the rubber tube (5) to a syringe, use the syringe to suck inert gas, and the suction volume is the same as the volume of the preset experimental gas to be introduced. After sucking the inert gas, inject the preset experimental gas through the syringe; after injecting the preset experimental gas, connect the other end of the rubber tube (5) to the air inlet of the aluminum - foil gas collection bag (6) again and seal it.

5. The method for using a greenhouse gas collection device for anaerobic cultivation of soil according to claim 3, characterized in that, When destructive sampling is required, the experimental soil (7) in the screw-mouth glass bottle (1) is taken out for detection after S3; when continuous collection of the gas in the screw-mouth glass bottle (1) is needed, another aluminum foil gas collection bag (6) is connected to the reducer straight hose joint (4), and S2 and S3 are repeated.

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