Fermented compositions with mild exfoliating effects, their preparation methods and applications
The fermented composition, formed by fermenting plant extracts such as milk thistle extract and specific bacterial strains, solves the problems of high irritation and poor stability of existing exfoliation methods, achieving a gentle yet highly effective exfoliation effect, and is suitable for use in skin care products.
Patent Information
- Application Number
- CN202411057684.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-02
- Publication Date
- 2025-10-28
- Estimated Expiration
- 2044-08-02
AI Technical Summary
Existing exfoliation methods suffer from problems such as high irritation, poor stability, and high risk of skin damage. In particular, physical methods may cause fine scratches, chemical methods are prone to allergic reactions, and biological methods have unstable effects.
A compound extract of milk thistle extract, kudzu root extract, sophora flower extract, anemarrhena extract, echinacea extract, wolfberry fruit extract, and mucor extract is combined with fermentation by Lactobacillus reuteri, Streptococcus thermophilus, Bifidobacterium animalis, Bacillus licheniformis, and Bifidobacterium bifidum, and papain and preservatives are added to form a mild exfoliating fermentation composition.
It achieves a gentle and stable exfoliating effect, reduces skin irritation, improves product stability and safety, and is suitable for addition to skin care products.
Smart Images
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Abstract
Description
Technical Field
[0001] This invention relates to the field of skincare products, and in particular to a fermented composition with gentle exfoliating properties, its preparation method, and its application. Background Technology
[0002] With the fast pace of life and changes in the living environment, people face increasing pressure and challenges in their daily lives, including but not limited to increased stress, insufficient sleep, irregular schedules, and unbalanced diets. These factors lead to a decline in the skin's self-regulating ability, thereby triggering a series of skin problems.
[0003] Under normal circumstances, the stratum corneum cells naturally renew themselves every 28 days, and the stratum corneum sheds naturally with each metabolism. However, due to skin aging, stress, lack of sleep, diet, and other factors, this metabolic rate often slows down. If old cells don't shed in time, they accumulate and form dead skin cells, leading to various skin problems. For example, it can prevent sebaceous glands from properly excreting, causing oil to clog pores. Dust and bacteria can then mix with the oil in the pores, resulting in inflammation and redness. Therefore, proper exfoliation is essential.
[0004] Currently, there are three main types of exfoliation methods on the market: physical methods, chemical methods, and biological methods. Physical methods primarily involve using various abrasive particles to scrub the skin surface to achieve an exfoliating effect. The effectiveness of these abrasive particles is affected by the particle size and the massage pressure; however, the massage process may cause minor scratches, especially around the eyes and on sensitive skin with visible redness, or in areas with a thin stratum corneum, potentially damaging the skin or even causing inflammation. Chemical methods mainly refer to alpha-hydroxy acids (AHAs) and beta-hydroxy acids (Salicylic acid). AHAs have a small molecular weight and are water-soluble, requiring a high concentration to achieve the desired effect. They are also highly irritating and prone to causing allergic reactions. Salicylic acid has a relatively large molecular weight and is a lipid-soluble acid, allowing it to penetrate more easily into oily pores. It also dissolves lipids in the stratum corneum, breaking down the stratum corneum structure, weakening the skin's defenses, and potentially causing skin allergies, dryness, peeling, redness, and stinging. Biological exfoliation methods often use enzymes, which hydrolyze proteins and specific proteases disrupt cell-cell interactions, accelerating exfoliation. However, in practical applications, these methods suffer from low stability and poor effectiveness. Therefore, there is still room for improvement. Summary of the Invention
[0005] In order to make exfoliating products both gentle, stable and effective at removing dead skin cells, this application provides a fermented composition with gentle exfoliating effect, its preparation method and its application.
[0006] In a first aspect, this application provides a method for preparing a fermented composition with mild exfoliating effects, using the following technical solution:
[0007] A method for preparing a fermented composition with mild exfoliating properties includes the following steps:
[0008] Step 1) Mix milk thistle extract, kudzu root extract, sophora flower extract, anemarrhena extract, echinacea extract, wolfberry fruit extract, mucor extract, and solvent evenly to obtain a compound extract.
[0009] Step 2): Add compound bacteria to the compound extract, ferment, sterilize, and obtain fermentation product;
[0010] Step 3) Add papain and preservative to the fermentation product, mix well, and obtain a fermentation composition with mild exfoliating effect.
[0011] The compound bacteria are a combination of Lactobacillus reuteri, Streptococcus thermophilus, Bifidobacterium animalis, Bacillus licheniformis, and Bifidobacterium bifidum.
[0012] By adopting the above technical solution, a compound extract is formed by combining milk thistle extract, kudzu root extract, sophora japonica flower extract, anemarrhena asphodeloides extract, echinacea extract, wolfberry fruit extract, and *Mucor* extract. Fermentation is then carried out using *Lactobacillus reuteri*, *Streptococcus thermophilus*, *Bifidobacterium animalis*, *Bacillus licheniformis*, and *Bifidobacterium bifidum*. This allows the active ingredients in each extract, the secretions of each bacterium, and papain to work synergistically, effectively promoting the shedding of the stratum corneum and achieving a good exfoliating effect with minimal skin irritation. The exfoliation is very gentle, and the synergistic effect of various active ingredients and secretions also improves the stability of the fermented composition, resulting in a high-quality product.
[0013] Preferably, the mass ratio of milk thistle extract, kudzu root extract, sophora japonica flower extract, anemarrhena asphodeloides extract, echinacea extract, wolfberry fruit extract, mucor extract, and solvent is 10-12:3-5:7-9:2-4:13-15:8-10:14-16:150-160.
[0014] By adopting the above technical solution and specifically selecting the mass ratio of milk thistle extract, kudzu root extract, sophora japonica flower extract, anemarrhena asphodeloides extract, echinacea extract, wolfberry fruit extract, and mucor extract, the active ingredients in each composition work together more effectively, thus achieving a better and more efficient exfoliation effect.
[0015] Preferably, the mass ratio of Lactobacillus reuteri, Streptococcus thermophilus, Bifidobacterium animalis, Bacillus licheniformis, and Bifidobacterium bifidum is 1-2:4-6:1-2:3-4:2-3.
[0016] By adopting the above technical solution and specifically selecting the mass ratio of Lactobacillus reuteri, Streptococcus thermophilus, Bifidobacterium animalis, Bacillus licheniformis, and Bifidobacterium bifidum, the activity of each bacterium is higher, the fermentation efficiency is higher, and it can better decompose irritating substances, making the fermentation composition milder, less likely to cause skin damage, and safer.
[0017] Preferably, the mass ratio of the compound extract to the compound bacteria is 207-231:0.0011-0.0017.
[0018] By adopting the above technical solution and specifically selecting the mass ratio of compound extract and compound bacteria, the fermentation effect is better, while the cost can be effectively controlled, resulting in high economic value.
[0019] Preferably, the mass ratio of the compound extract to papain is 207-231:6-8.
[0020] By adopting the above technical solution and specifically selecting the mass ratio of compound extract to papain, the exfoliation effect is improved.
[0021] Preferably, in step 2), the fermentation temperature is 36-38℃.
[0022] By adopting the above technical solution and specifically selecting the fermentation temperature, the fermentation efficiency is higher, irritating substances are better removed, and the fermentation composition has a better effect on removing dead skin and is less irritating.
[0023] Preferably, in step 2), the fermentation time is 6-8 days.
[0024] By adopting the above technical solution and specifically selecting the fermentation time, the fermentation is more complete, resulting in higher product quality.
[0025] Preferably, in step 2), sterilization is performed by filtration through a 0.22-micron filter membrane.
[0026] By adopting the above technical solution, the sterilization effect is better, and the stability and safety of the fermentation composition are both high.
[0027] Preferably, the preservative is a compound of p-hydroxyacetophenone and hexanediol, and the mass ratio of the compound extract to p-hydroxyacetophenone and hexanediol is 207-231:0.3-0.5:0.4-0.6.
[0028] By adopting the above technical solutions, the product quality is better and the storage period is longer.
[0029] Secondly, this application provides a fermented composition with mild exfoliating effect, using the following technical solution:
[0030] A fermented composition with mild exfoliating effect is prepared by the above-described method for preparing a fermented composition with mild exfoliating effect.
[0031] By adopting the above technical solution, the fermented composition with gentle exfoliation effect can achieve rapid exfoliation, and the product has low irritation, is gentle on the skin, has high safety, and is of good quality.
[0032] Thirdly, this application provides an application of a fermented composition with mild exfoliating properties, employing the following technical solution:
[0033] An application of the above-mentioned fermented composition with mild exfoliating effect, wherein the fermented composition with mild exfoliating effect is added to a skin care product to prepare a skin care product with mild exfoliating effect, wherein the fermented composition with mild exfoliating effect has a mass percentage content of 2-10% in the skin care product.
[0034] By adopting the above technical solution, the fermented composition with gentle exfoliating effect can be added to skin care products, which can make the skin care products have a good exfoliating effect, and are gentle and non-irritating to the skin with high safety.
[0035] In summary, this application has the following beneficial effects:
[0036] 1. This application uses milk thistle extract, kudzu root extract, sophora japonica flower extract, anemarrhena asphodeloides extract, echinacea extract, wolfberry fruit extract, and Mucor extract to form a compound extract. Through fermentation with Lactobacillus reuteri, Streptococcus thermophilus, Bifidobacterium animalis, Bacillus licheniformis, and Bifidobacterium bifidum, the active ingredients in each extract, the secretions of each bacterium, and papain work synergistically to effectively promote the shedding of the stratum corneum, resulting in a good exfoliating effect with minimal skin irritation. The exfoliation is very gentle, and the synergistic effect of various active ingredients and secretions also improves the stability of the fermented composition, leading to better product quality.
[0037] 2. In this application, the preferred method is to select the specific mass ratio of milk thistle extract, kudzu root extract, sophora japonica flower extract, anemarrhena asphodeloides extract, echinacea extract, wolfberry fruit extract, and mucor extract to make the active ingredients in each composition work together better and achieve a more efficient exfoliation effect.
[0038] 3. In this application, the preferred method is to select the specific mass ratio of Lactobacillus reuteri, Streptococcus thermophilus, Bifidobacterium animalis, Bacillus licheniformis, and Bifidobacterium bifidum, so that the activity of each bacterium is higher, the fermentation efficiency is higher, and the decomposition of irritating substances is better, making the fermentation composition milder, less likely to cause damage to the skin, and safer. Detailed Implementation
[0039] The present application will be further described in detail below with reference to the embodiments.
[0040] Example 1
[0041] A method for preparing a fermented composition with mild exfoliating properties includes the following steps:
[0042] Step 1): Add 10kg milk thistle extract, 3kg kudzu root extract, 7kg sophora japonica flower extract, 2kg anemarrhena asphodeloides extract, 13kg echinacea extract, 8kg wolfberry fruit extract, 14kg Mucor miltiorrhiza extract, and 150kg solvent into a stirring vessel. Stir at 120r / min for 30min until the mixture is homogeneous to obtain the compound extract.
[0043] Step 2): Add 10 mg of Lactobacillus reuteri, 40 mg of Streptococcus thermophilus, 10 mg of Bifidobacterium animalis, 30 mg of Bacillus licheniformis, and 20 mg of Bifidobacterium bifidum to the compound extract. Stir continuously at 10 r / min, maintain a constant temperature of 36℃, and ferment for 8 days. Filter through a 0.22 micron filter membrane to complete sterilization and obtain the fermentation product.
[0044] Step 3): Add 6 kg of papain, 0.5 kg of p-hydroxyacetophenone, and 1.2 kg of hexanediol to the fermentation product. Stir at 120 r / min for 10 min until well mixed to obtain a fermentation composition with mild exfoliating effect.
[0045] Milk thistle extract was purchased from Hebei Hongtao Bioengineering Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0046] The kudzu root extract was purchased from Shandong Pingju Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0047] The Sophora japonica flower extract was purchased from Lanzhou Waterles Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0048] The Anemarrhena asphodeloides extract was purchased from Shaanxi Xintianyu Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0049] The echinacea extract was purchased from Shandong Pingju Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0050] The wolfberry fruit extract was purchased from Lanzhou Waterles Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0051] The extract of *Mucor* was purchased from Liptek Corporation in the United States.
[0052] Lactobacillus reuteri was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-67295.
[0053] The thermophilic streptococcus was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-72768.
[0054] Bifidobacterium animalis was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-67241.
[0055] Bacillus licheniformis was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-67637.
[0056] Bifidobacterium bifidum was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-67244.
[0057] Papain was purchased from Zhengzhou Kema Biotechnology Co., Ltd. as packaged papain.
[0058] p-Hydroxyacetophenone is commercially available, CAS No.: 99-93-4.
[0059] Hexanediol is commercially available, CAS No.: 629-11-8.
[0060] Example 2
[0061] A method for preparing a fermented composition with mild exfoliating properties includes the following steps:
[0062] Step 1): Add 11kg milk thistle extract, 4kg kudzu root extract, 8kg sophora japonica flower extract, 3kg anemarrhena asphodeloides extract, 14kg echinacea extract, 9kg wolfberry fruit extract, 15kg Mucor miltiorrhiza extract, and 155kg solvent into a stirring vessel. Stir at 120r / min for 30min until the mixture is homogeneous to obtain the compound extract.
[0063] Step 2): Add 15mg of Lactobacillus reuteri, 50mg of Streptococcus thermophilus, 15mg of Bifidobacterium animalis, 35mg of Bacillus licheniformis, and 25mg of Bifidobacterium bifidum to the compound extract, stir continuously at 10r / min, keep the temperature at 37℃, ferment for 7 days, filter through a 0.22-micron filter membrane to complete sterilization, and obtain the fermentation product.
[0064] Step 3): Add 7 kg of papain, 0.6 kg of p-hydroxyacetophenone, and 1.5 kg of hexanediol to the fermentation product. Stir at 120 r / min for 10 min until well mixed to obtain a fermentation composition with mild exfoliating effect.
[0065] Milk thistle extract was purchased from Hebei Hongtao Bioengineering Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0066] The kudzu root extract was purchased from Shandong Pingju Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0067] The Sophora japonica flower extract was purchased from Lanzhou Waterles Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0068] The Anemarrhena asphodeloides extract was purchased from Shaanxi Xintianyu Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0069] The echinacea extract was purchased from Shandong Pingju Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0070] The wolfberry fruit extract was purchased from Lanzhou Waterles Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0071] The extract of *Mucor* was purchased from Liptek Corporation in the United States.
[0072] Lactobacillus reuteri was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-67295.
[0073] The thermophilic streptococcus was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-72768.
[0074] Bifidobacterium animalis was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-67241.
[0075] Bacillus licheniformis was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-67637.
[0076] Bifidobacterium bifidum was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-67244.
[0077] Papain was purchased from Zhengzhou Kema Biotechnology Co., Ltd. as packaged papain.
[0078] p-Hydroxyacetophenone is commercially available, CAS No.: 99-93-4.
[0079] Hexanediol is commercially available, CAS No.: 629-11-8.
[0080] Example 3
[0081] A method for preparing a fermented composition with mild exfoliating properties includes the following steps:
[0082] Step 1): Add 12kg milk thistle extract, 5kg kudzu root extract, 9kg sophora japonica flower extract, 4kg anemarrhena asphodeloides extract, 15kg echinacea extract, 10kg wolfberry fruit extract, 16kg Mucor miltiorrhiza extract, and 160kg solvent into a stirring vessel. Stir at 120r / min for 30min until the mixture is homogeneous to obtain the compound extract.
[0083] Step 2): Add 20 mg of Lactobacillus reuteri, 60 mg of Streptococcus thermophilus, 20 mg of Bifidobacterium animalis, 10 mg of Bacillus licheniformis, and 30 mg of Bifidobacterium bifidum to the compound extract. Stir continuously at 10 r / min, keep the temperature at 38℃, and ferment for 6 days. Filter through a 0.22 micron filter membrane to complete sterilization and obtain the fermentation product.
[0084] Step 3): Add 8 kg of papain, 0.5 kg of p-hydroxyacetophenone, and 1.8 kg of hexanediol to the fermentation product. Stir at 120 r / min for 10 min until well mixed to obtain a fermentation composition with mild exfoliating effect.
[0085] Milk thistle extract was purchased from Hebei Hongtao Bioengineering Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0086] The kudzu root extract was purchased from Shandong Pingju Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0087] The Sophora japonica flower extract was purchased from Lanzhou Waterles Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0088] The Anemarrhena asphodeloides extract was purchased from Shaanxi Xintianyu Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0089] The echinacea extract was purchased from Shandong Pingju Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0090] The wolfberry fruit extract was purchased from Lanzhou Waterles Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0091] The extract of *Mucor* was purchased from Liptek Corporation in the United States.
[0092] Lactobacillus reuteri was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-67295.
[0093] The thermophilic streptococcus was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-72768.
[0094] Bifidobacterium animalis was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-67241.
[0095] Bacillus licheniformis was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-67637.
[0096] Bifidobacterium bifidum was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-67244.
[0097] Papain was purchased from Zhengzhou Kema Biotechnology Co., Ltd. as packaged papain.
[0098] p-Hydroxyacetophenone is commercially available, CAS No.: 99-93-4.
[0099] Hexanediol is commercially available, CAS No.: 629-11-8.
[0100] Comparative Example 1
[0101] A method for preparing a fermented composition with mild exfoliating properties, differing from Example 2 only in that:
[0102] In the compound extract, milk thistle extract was replaced with an equal amount of dew grass extract.
[0103] The dewdrop extract was purchased from Xi'an Green Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0104] Comparative Example 2
[0105] A method for preparing a fermented composition with mild exfoliating properties, differing from Example 2 only in that:
[0106] In the compound extract, sugarcane extract was used to replace kudzu root extract in an equal amount.
[0107] The sugarcane extract was purchased from Xi'an Ruierli Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0108] Comparative Example 3
[0109] A method for preparing a fermented composition with mild exfoliating properties, differing from Example 2 only in that:
[0110] In the compound extract, tea tree flower extract was used to replace sophora flower extract in an equal amount.
[0111] The tea tree flower extract was purchased from Shanyang Lianfeng Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0112] Comparative Example 4
[0113] A method for preparing a fermented composition with mild exfoliating properties, differing from Example 2 only in that:
[0114] In the compound extract, olive extract was used to replace anemarrhena extract in an equal amount.
[0115] Olive oil extract was purchased from Shanyang Lianfeng Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0116] Comparative Example 5
[0117] A method for preparing a fermented composition with mild exfoliating properties, differing from Example 2 only in that:
[0118] In the compound extract, an equal amount of Echinacea extract was replaced with Spatholobus suberectus extract.
[0119] The extract of *Symplocos buergeriana* was purchased from Lanzhou Waterles Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0120] Comparative Example 6
[0121] A method for preparing a fermented composition with mild exfoliating properties, differing from Example 2 only in that:
[0122] In the compound extract, fig extract was used to replace wolfberry extract in an equal amount.
[0123] The fig extract was purchased from Weinan Dongjiang Tiancheng Industrial Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0124] Comparative Example 7
[0125] A method for preparing a fermented composition with mild exfoliating properties, differing from Example 2 only in that:
[0126] In the compound extract, apple fruit extract was used to replace the Mucor extract in an equal amount.
[0127] Apple fruit extract was purchased from Hubei Xinyuhong Biomedical Technology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0128] Comparative Example 8
[0129] A method for preparing a fermented composition with mild exfoliating properties, differing from Example 2 only in that:
[0130] In the compound extract, milk thistle extract was replaced in equal amounts with dew grass extract, kudzu root extract was replaced in equal amounts with sugarcane extract, sophora japonica flower extract was replaced in equal amounts with tea tree flower extract, anemarrhena asphodeloides extract was replaced in equal amounts with olive extract, echinacea extract was replaced in equal amounts with pearly blue ear grass extract, wolfberry fruit extract was replaced in equal amounts with fig extract, and mucor extract was replaced in equal amounts with apple fruit extract.
[0131] The dewdrop extract was purchased from Xi'an Green Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0132] The sugarcane extract was purchased from Xi'an Ruierli Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0133] The tea tree flower extract was purchased from Shanyang Lianfeng Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0134] Olive oil extract was purchased from Shanyang Lianfeng Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0135] The extract of *Symplocos buergeriana* was purchased from Lanzhou Waterles Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0136] The fig extract was purchased from Weinan Dongjiang Tiancheng Industrial Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0137] Apple fruit extract was purchased from Hubei Xinyuhong Biomedical Technology Co., Ltd. It is a water-soluble extract with an extraction ratio of 10:1.
[0138] Comparative Example 9
[0139] A method for preparing a fermented composition with mild exfoliating properties, differing from Example 2 only in that:
[0140] In the compound bacteria, Lactobacillus acidophilus was used to replace Lactobacillus reuteri in an equal amount.
[0141] Lactobacillus acidophilus was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-52717.
[0142] Comparative Example 10
[0143] A method for preparing a fermented composition with mild exfoliating properties, differing from Example 2 only in that:
[0144] In the compound bacteria, Saccharomyces cerevisiae is used to replace Streptococcus thermophilus in an equal amount.
[0145] The brewing yeast was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-53342.
[0146] Comparative Example 11
[0147] A method for preparing a fermented composition with mild exfoliating properties, differing from Example 2 only in that:
[0148] In the compound bacteria, Clostridium butyricum is used to replace Bifidobacterium animalis in an equal amount.
[0149] Clostridium butyricum was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-85310.
[0150] Comparative Example 12
[0151] A method for preparing a fermented composition with mild exfoliating properties, differing from Example 2 only in that:
[0152] In the compound bacteria, Bacillus licheniformis was replaced in an equal amount with Fermentation Cellularis.
[0153] Fermentation cytosporum was purchased from Beijing Bio-Biobio Technology Co., Ltd., platform number: Bio-81907.
[0154] Comparative Example 13
[0155] A method for preparing a fermented composition with mild exfoliating properties, differing from Example 2 only in that:
[0156] In the compound bacteria, Aspergillus niger is used to replace Bifidobacterium bifidum in an equal amount.
[0157] Aspergillus niger was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-52178.
[0158] Comparative Example 14
[0159] A method for preparing a fermented composition with mild exfoliating properties, differing from Example 2 only in that:
[0160] In the compound bacteria, Lactobacillus acidophilus replaced Lactobacillus reuteri in equal amounts, Saccharomyces cerevisiae replaced Streptococcus thermophilus in equal amounts, Clostridium butyricum replaced Bifidobacterium animalis in equal amounts, Fibromosomalemia fermentum replaced Bacillus licheniformis in equal amounts, and Aspergillus niger replaced Bifidobacterium bifidum in equal amounts.
[0161] Lactobacillus acidophilus was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-52717.
[0162] The brewing yeast was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-53342.
[0163] Clostridium butyricum was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-85310.
[0164] Fermentation cytosporum was purchased from Beijing Bio-Biobio Technology Co., Ltd., platform number: Bio-81907.
[0165] Aspergillus niger was purchased from Beijing Bio-Bio Biotechnology Co., Ltd., platform number: Bio-52178.
[0166] Experiment 1
[0167] Stability test
[0168] The fermented compositions with mild exfoliating effects of each embodiment and comparative example were prepared into test samples and tested according to the following test requirements. The test results are shown in Table 1.
[0169] The samples at room temperature were left to stand at 25°C for comparative testing.
[0170] Cold resistance test: -18℃, placed for 1 month.
[0171] Thermal cycling: -18℃; 25℃; 42℃, 24 hours at each temperature, for a total of 5 cycles.
[0172] High-temperature acceleration: The test conditions were 42℃ ± 1℃ for 1 month.
[0173] Test samples: fermented compositions with mild exfoliating effects in each embodiment and comparative example.
[0174] Table 1
[0175]
[0176]
[0177] According to the data in Table 1, the stability of each embodiment was normal when subjected to high and low temperature cycling, heat resistance, cold resistance and room temperature tests, while the heat resistance stability of each comparative example was poor. It can be seen that the stability of each embodiment is significantly better than that of each comparative example.
[0178] Experiment 2
[0179] In vitro exfoliation efficacy test (3D skin model to determine the amount of protein washed off)
[0180] Normal 3D skin models were transferred to 6-well plates pre-filled with 0.9 mL of EpiGrowth medium. The plates were divided into: a blank control group and test samples (each example and comparative example), with a sample concentration of 4%. 25 μL of the test sample was added to the surface of each test model, spread evenly, and incubated in a CO2 incubator (37℃, 5% CO2) for 24 h. After incubation, the model surface was cleaned with sterile PBS solution to remove any remaining test material. Sterile cotton swabs were used to wipe away any residual liquid inside and outside the model. The 3D skin model was then rubbed for 2 minutes using a syringe rubber sieve to simulate the shedding of the surface keratin. The model was then cleaned again, and the cleansing solution (0.1% Triton X-100) was collected. The total protein content was determined according to the instructions of the BCA protein assay kit. Measuring the amount of eluted protein reflects the sample's ability to exfoliate the surface keratin.
[0181] Test sample: 4g of fermented composition with mild exfoliating effect, 96g of deionized water, mixed evenly.
[0182] The experimental data are shown in Table 2.
[0183] Table 2
[0184]
[0185]
[0186] According to the data comparison in Table 2, the total protein content exfoliated in each embodiment is significantly higher than that in each pair of proportions. Therefore, the exfoliation efficacy of each embodiment is significantly better than that of each pair of proportions.
[0187] Experiment 3
[0188] Mildness test - human patch test
[0189] Human skin patch test, the trial method is based on: "Cosmetic Safety Technical Specifications" (2015 edition).
[0190] General testing method: Select a suitable patch tester and use a closed patch test method. Select at least 30 qualified volunteers to participate in the test. Add the test substance and control sample to the square chamber of the patch tester. Apply the patch tester containing the test substance and control sample to the inner forearm of the subject, and gently press it with the palm of your hand to make it evenly applied to the skin. After 24 hours, remove the patch tester and observe the skin reaction according to the standards in Table 3 at 30 minutes (after the indentation disappears), 24 hours, and 48 hours, and record the observation results.
[0191] Following the general method described above, 34 qualified volunteers were selected to participate in the test. The test samples prepared from the fermented compositions with mild exfoliating effects of each embodiment and comparative example were used as test substances, while sterile deionized water was used as the control sample. The patch test apparatus containing the test substance and control sample was applied to the inner forearm of the subjects and removed after 24 hours. Skin reactions were observed 30 minutes, 24 hours, and 48 hours after removal of the patch test apparatus, according to the grading criteria for closed patch tests in Table 3. The observation results are recorded as shown in Table 4.
[0192] Test sample: 10g of fermented composition with mild exfoliating effect, 90g of deionized water, mixed evenly to obtain the test sample.
[0193] Table 3
[0194]
[0195]
[0196] Table 4
[0197]
[0198]
[0199] According to the data in Table 4, all examples were negative, while all comparative examples showed grade 1-2 reactions. It can be seen that the fermentation compositions of the comparative examples were somewhat irritating, while the examples were basically non-irritating and milder.
[0200] In summary, the various embodiments involve mixing milk thistle extract, kudzu root extract, sophora japonica bud extract, anemarrhena asphodeloides root extract, purple coneflower extract, Ningxia wolfberry fruit extract, and *Mucor* extract, followed by fermentation with *Lactobacillus reuteri*, *Streptococcus thermophilus*, *Bifidobacterium licheniformis*, and *Bifidobacterium bifidum*. The active ingredients and fermentation products, combined with papain, promote the shedding of the stratum corneum, resulting in rapid exfoliation. Furthermore, the combination of specific bacteria effectively reduces irritants during fermentation, leading to a lower irritation level in the fermented composition. This ensures better stability of the various components, maintaining product quality over a long period and achieving a gentle yet highly effective exfoliation. The product exhibits high stability and safety.
[0201] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.
Claims
1. A method for preparing a fermented composition with mild exfoliating properties, characterized in that: Includes the following steps: Step 1) Mix milk thistle extract, kudzu root extract, sophora flower extract, anemarrhena extract, echinacea extract, wolfberry fruit extract, mucor extract, and solvent evenly to obtain a compound extract; Step 2), add compound bacteria to the compound extract, ferment, sterilize, and obtain fermentation product; Step 3) Add papain and preservative to the fermentation product, mix well, and obtain a fermentation composition with mild exfoliating effect; The compound bacteria are a combination of Lactobacillus reuteri, Streptococcus thermophilus, Bifidobacterium animalis, Bacillus licheniformis, and Bifidobacterium bifidum. The mass ratio of milk thistle extract, kudzu root extract, sophora japonica flower extract, anemarrhena asphodeloides extract, echinacea extract, wolfberry fruit extract, mucor extract, and solvent is 10-12:3-5:7-9:2-4:13-15:8-10:14-16:150-160. The mass ratio of Lactobacillus reuteri, Streptococcus thermophilus, Bifidobacterium animalis, Bacillus licheniformis, and Bifidobacterium bifidum is 1-2:4-6:1-2:3-4:2-3; The mass ratio of the compound extract to the compound bacteria is 207-231:0.0011-0.0017; The mass ratio of the compound extract to papain is 207-231:6-8.
2. The method for preparing a fermented composition with mild exfoliating effect according to claim 1, characterized in that: In step 2), the fermentation temperature is 36-38℃.
3. The method for preparing a fermented composition with mild exfoliating effect according to claim 1, characterized in that: In step 2), the fermentation time is 6-8 days.
4. The method for preparing a fermented composition with mild exfoliating effect according to claim 1, characterized in that: In step 2), sterilization is performed by filtering through a 0.22-micron filter membrane.
5. A fermented composition with mild exfoliating properties, characterized in that: It is prepared by the method of any one of claims 1-4 of the fermented composition having a mild exfoliating effect.
6. The application of a fermented composition with mild exfoliating effect according to claim 5, characterized in that: The fermented composition with mild exfoliating effect is added to a skin care product to prepare a skin care product with mild exfoliating effect, wherein the fermented composition with mild exfoliating effect has a mass percentage content of 2-10% in the skin care product.
Citation Information
Patent Citations
Cosmetic use of microorganisms
WO2009031099A2