A chicken bile extract taurochenodeoxycholic acid composition and its application in preparing an anti-stress drug for improving the egg-laying period of rutin chickens

Abstract

The invention discloses a chicken bile extract taurochenodeoxycholic acid composition and application thereof in preparing an anti-stress drug for improving the laying period of rutin chickens, belonging to the technical field of animal feed additives. The chicken bile extract taurochenodeoxycholic acid composition consists of the following components by weight: 60-65 parts of a bile acid mixture and 35-40 parts of a composite additive; a preparation method of the bile acid mixture consists of the following steps: adsorbing bile acid, adsorbing curcumin, preparing a coating solution, and coating; a preparation method of the composite additive consists of the following steps: adsorbing a water-soluble additive, adsorbing soy protein, adsorbing a fat-soluble additive, preparing a coating solution, and coating; the chicken bile extract taurochenodeoxycholic acid composition can improve the anti-stress ability and feed intake of rutin chickens during the laying period, has strong storage stability, and is easy to be evenly mixed with feed.

Description

Technical Field

[0001] The present invention relates to the technical field of animal feed additives, and in particular to a chicken bile extract taurochenodeoxycholic acid composition and an application thereof in preparing an anti-stress drug for improving the laying period of rutin chickens. Background Art

[0002] Stress is the physiological response of the animal body to stressors. In intensive farming models, stress in poultry is unavoidable and long-term. In modern poultry production, stress is one of the main reasons for the deterioration of poultry health and decreased production performance.

[0003] Rudina fowl are adorable small chickens with a petite body and docile temperament, making them popular family pets. The nutritional value of Rudina eggs, the ornamental value of Rudina fowl, and their value as companions for children are outstanding. In the breeding process of Rudina fowl, the main stressors include environmental factors, feeding and management factors, disease factors, genetic factors, nutritional factors, and medication factors.

[0004] When rudin chickens encounter stressors, they will cause nonspecific reactions in the hypothalamus, pituitary gland, and adrenal cortex, stimulating the body to avoid self-harm through neurohumoral regulation, and ultimately adapt to and dilute such stressors. However, if the stimulation of the stressor is too strong and the stimulation lasts too long, far exceeding the body's tolerance limit, the rudin chickens will lose their ability to cope and produce a stress response.

[0005] The stress response of rudin chickens has a greater impact on rudin chickens in the laying period. Specifically, it will reduce the fertilization ability and productivity of female rudin chickens, reduce the mating times of male rudin chickens, and thus lead to a decrease in egg production rate and lower quality of fertilized eggs; at the same time, it will also manifest as a decrease in the body's immunity and susceptibility to infection, which will lead to an increase in the incidence rate.

[0006] Therefore, people are paying more and more attention to improving the anti-stress ability of rudin chickens during the breeding process. At present, the methods used to improve the anti-stress ability of rudin chickens include improving feeding management, adjusting the egg picking time, reducing the number of manure cleaning times, improving breeding cages, and using silent equipment. In addition, some breeding companies add feed additives with vitamins, trace elements, minerals, proteins, γ-aminobutyric acid, betaine, Chinese herbal medicine extracts, etc. as main ingredients to the feed of rudin chickens to reduce the harm of stressors to rudin chickens and improve the anti-stress ability of rudin chickens.

[0007] Bile in the gallbladder has extremely high medicinal value. Bile acids, as an important component of bile, are divided into free bile acids and conjugated bile acids. Free bile acids mainly include cholic acid, deoxycholic acid, chenodeoxycholic acid, and lithocholic acid, while conjugated bile acids mainly include glycocholic acid, glycochenodeoxycholic acid, taurocholic acid, and taurochenodeoxycholic acid. The inventors have discovered that taurochenodeoxycholic acid, a bile acid found in chickens, can improve the stress resistance of roosters during their egg-laying period. Therefore, in the breeding process of roosters, an increasing number of breeding companies are using bile acids as feed additives for roosters during their egg-laying period.

[0008] Most broiler chicken gallbladders are discarded as waste and have an extremely low utilization rate. Therefore, the inventors used bile from broiler chicken gallbladders as raw material, extracted and purified it, and obtained bile acid with a mass content of 5-10% taurochenodeoxycholic acid, and used this bile acid as a feed additive for roosters during the laying period. However, there are the following problems in use: first, due to the low mass content of taurochenodeoxycholic acid in bile acid, a large amount of bile acid needs to be added to the feed to achieve good stress resistance. The addition of a large amount of bile acid will reduce the rooster's absorption of vitamins, trace elements, minerals, and protein, resulting in a decrease in egg production. Second, bile acid tastes slightly bitter and has poor palatability, which will reduce the rooster's feed intake. Third, bile acid easily absorbs moisture, resulting in poor storage stability. Fourth, bile acid is a brown powder, and rooster feed is generally granular. Directly mixing bile acid with feed will result in uneven mixing.

[0009] To address these issues, the inventors have attempted to prepare a granular feed additive by compounding bile acid with vitamins, trace elements, minerals, proteins, flavoring agents, and moisture-proofing agents. The combined effects of bile acid, vitamins, trace elements, minerals, and proteins reduce the dosage of taurochenodeoxycholic acid and improve the stress resistance of roe fowl. The flavoring agent increases the roe fowl's feed intake. The moisture-proofing agent reduces the hygroscopicity of the granular feed additive. By processing the granular feed additive to the same particle size as the feed, the granular feed additive improves mixing uniformity with the feed. However, the following problems still exist: First, when the prepared granular feed additive is used for rudin chickens during their laying period, bile acid is simply mixed with vitamins, trace elements, minerals, and proteins. There is a mutual influence between bile acid and vitamins, trace elements, minerals, and proteins, resulting in a poor effect on improving the anti-stress ability of rudin chickens; Second, there is still the problem that the addition of bile acid will reduce the absorption of vitamins, trace elements, minerals, and proteins by rudin chickens, resulting in a decrease in the egg-laying ability of rudin chickens; Third, the desiccant has an adsorption effect on the components in the granular feed additive, which will affect the absorption of rudin chickens and result in a decrease in the egg-laying ability of rudin chickens. Summary of the Invention

[0010] In response to the shortcomings of the existing technology, the present invention provides a chicken bile extract taurochenodeoxycholic acid composition and its use in the preparation of an anti-stress drug for improving the laying period of rutin chickens. The chicken bile extract taurochenodeoxycholic acid composition of the present invention can improve the anti-stress ability and feed intake of rutin chickens during the laying period, and has strong storage stability and is easy to mix evenly with feed.

[0011] In order to solve the above technical problems, the technical solutions adopted by the present invention are as follows:

[0012] A chicken bile extract taurochenodeoxycholic acid composition, comprising the following components by weight: 60-65 parts of a bile acid mixture and 35-40 parts of a composite additive;

[0013] The preparation method of the bile acid mixture comprises the following steps: adsorbing bile acid, adsorbing curcumin, preparing a coating solution, and coating;

[0014] The bile acid adsorption comprises mixing bile acid and anhydrous ethanol, stirring at room temperature at a stirring speed of 100-150 rpm for 2-3 hours, adding light calcium carbonate, stirring for 24-26 hours, filtering, collecting the filter residue, and drying the filter residue to obtain calcium carbonate adsorbing bile acid;

[0015] In the adsorbed bile acid, the mass ratio of bile acid, anhydrous ethanol, and light calcium carbonate is 23-25:1000-1100:85-90;

[0016] The mass content of taurochenodeoxycholic acid in the bile acid is 6%;

[0017] The particle size of the light calcium carbonate is 80 μm;

[0018] The curcumin adsorption process comprises mixing curcumin and anhydrous ethanol, stirring the mixture at room temperature at a stirring speed of 100-150 rpm for 4-5 hours, adding calcium carbonate adsorbing bile acid, stirring the mixture for 16-18 hours, filtering, collecting the filter residue, and drying the filter residue to obtain calcium carbonate adsorbing curcumin;

[0019] In the adsorbed curcumin, the mass ratio of curcumin, anhydrous ethanol, and calcium carbonate for adsorbing bile acid is 13-15:3200-3500:105-110;

[0020] In the method for preparing the bile acid mixture, a coating solution is prepared by mixing hypromellose, polyethylene glycol 6000, sodium carboxymethyl cellulose, magnesium stearate, calcium chloride, anhydrous ethanol, and deionized water, and stirring the mixture at room temperature at a stirring speed of 300-400 rpm for 40-50 minutes to obtain a coating solution;

[0021] In the preparation method of the bile acid mixture, in the coating solution, the mass ratio of hydropropyl methylcellulose, polyethylene glycol 400, sodium carboxymethyl cellulose, magnesium stearate, calcium chloride, anhydrous ethanol, and deionized water is 30-35:12-15:2-2.5:10-14:1-1.2:70-75:255-270;

[0022] The particle size of the magnesium stearate is 5 μm;

[0023] The coating in the preparation method of the bile acid mixture comprises adding the curcumin-adsorbed calcium carbonate into a coating machine, coating with a coating liquid, controlling the flow rate of the coating liquid to be 0.7-0.8 L / min, the inlet air temperature to be 45-50° C., and the outlet air temperature to be room temperature, to obtain the bile acid mixture;

[0024] In the coating of the preparation method of the bile acid mixture, the mass ratio of calcium carbonate adsorbing curcumin to the coating solution is 90:27-29;

[0025] The preparation method of the composite additive comprises the following steps: adsorbing a water-soluble additive, adsorbing soy protein, adsorbing a fat-soluble additive, preparing a coating solution, and coating;

[0026] The water-soluble additive adsorption method comprises mixing vitamin C, vitamin B1, vitamin B2, vitamin B5, gamma-aminobutyric acid, and deionized water, stirring the mixture at a stirring speed of 100-150 rpm for 40-50 minutes at room temperature, adding light calcium carbonate, stirring the mixture for 9-10 hours, filtering, collecting the filter residue, and drying the filter residue to obtain calcium carbonate adsorbing the water-soluble additive;

[0027] In the adsorption water-soluble additive, the mass ratio of vitamin C, vitamin B1, vitamin B2, vitamin B5, gamma-aminobutyric acid, deionized water, and light calcium carbonate is 2-3:1-1.3:1-1.3:2-2.5:47-50:2000-2400:180-200;

[0028] The particle size of the light calcium carbonate is 80 μm;

[0029] The soy protein adsorption comprises mixing soy protein and deionized water, stirring at a stirring speed of 200-400 rpm at room temperature for 3-4 hours, stirring at a stirring speed of 100-200 rpm at 4-5° C. for 7-8 hours, centrifuging, taking the supernatant, mixing the supernatant with calcium carbonate adsorbed with a water-soluble additive, stirring at a stirring speed of 100-150 rpm at room temperature for 17-20 hours, filtering, taking the filter residue, and drying the filter residue to obtain calcium carbonate adsorbed with soy protein;

[0030] In the adsorbed soy protein, the mass ratio of soy protein, deionized water, and calcium carbonate for adsorbing water-soluble additives is 40-42:2300-2500:230-240;

[0031] The fat-soluble additive adsorption method comprises mixing vitamin A, vitamin D, vitamin E, curcumin, and anhydrous ethanol, stirring the mixture at a stirring speed of 200-300 rpm at room temperature for 4-5 hours, adding calcium carbonate adsorbed with soy protein, stirring the mixture for 17-20 hours, filtering, collecting the filter residue, and drying the filter residue to obtain calcium carbonate adsorbed with fat-soluble additives;

[0032] In the adsorbed fat-soluble additive, the mass ratio of vitamin A, vitamin D, vitamin E, curcumin, anhydrous ethanol, and calcium carbonate adsorbed with soy protein is 0.5-0.6:1-1.3:3-3.5:10-11:3000-3200:255-270;

[0033] The coating solution in the preparation method of the composite additive comprises mixing magnesium stearate, L-leucine, and a first portion of deionized water, stirring at a stirring speed of 200-300 rpm at room temperature for 3-4 hours, filtering, collecting the filter residue, mixing the filter residue with hypromellose, polyethylene glycol 6000, calcium chloride, anhydrous ethanol, and a second portion of deionized water, and stirring at a stirring speed of 300-400 rpm at room temperature for 40-50 minutes to obtain a coating solution;

[0034] In the coating solution prepared in the method for preparing the composite additive, the mass ratio of magnesium stearate, L-leucine, the first portion of deionized water, hydroxypropyl methylcellulose, polyethylene glycol 6000, calcium chloride, anhydrous ethanol, and the second portion of deionized water is 10-14:1.5-2:200-230:35-38:10-13:1-1.2:70-75:250-270;

[0035] The particle size of the magnesium stearate is 5 μm;

[0036] The coating in the preparation method of the composite additive comprises adding the calcium carbonate adsorbed with the fat-soluble additive into a coating machine, coating with a coating liquid, controlling the flow rate of the coating liquid to be 0.6-0.7 L / min, the inlet air temperature to be 45-50° C., and the outlet air temperature to be room temperature, to obtain the composite additive;

[0037] In the coating of the preparation method of the composite additive, the mass ratio of calcium carbonate adsorbing the fat-soluble additive to the coating solution is 90:30-32.

[0038] A method for preparing the aforementioned chicken bile extract taurochenodeoxycholic acid composition comprises mixing a bile acid mixture and a composite additive in prescribed parts by weight, and stirring the mixture at room temperature at a stirring speed of 30-50 rpm for 30-40 minutes to obtain the chicken bile extract taurochenodeoxycholic acid composition.

[0039] The invention discloses an application of the chicken bile extract taurochenodeoxycholic acid composition in the preparation of an anti-stress drug for improving the laying period of rutin chickens.

[0040] Compared with the prior art, the present invention has the following beneficial effects:

[0041] (1) The chicken bile extract taurochenodeoxycholic acid composition of the present invention can improve the stress resistance of rutin chickens during the laying period. The chicken bile extract taurochenodeoxycholic acid composition of the present invention is applied to the feeding of rutin chickens for 5 consecutive days, and a stressor is set on the second day of feeding. The total egg production, egg production rate, average daily feed intake per rutin chicken, and average daily water intake per rutin chicken on the third day did not decrease significantly compared with those on the second day.

[0042] (2) The chicken bile extract taurochenodeoxycholic acid composition of the present invention can increase the feed intake of rutin chickens during the laying period. The chicken bile extract taurochenodeoxycholic acid composition of the present invention is applied to the feeding of rutin chickens for 5 consecutive days, and a stressor is set on the second day of feeding. The average daily feed intake of each rutin chicken on the first day of feeding is 11.2-11.6 g, the average daily feed intake of each rutin chicken on the third day of feeding is 10.9-11.2 g, the average daily feed intake of each rutin chicken on the fourth day of feeding is 11.7-11.9 g, and the average daily feed intake of each rutin chicken on the fifth day of feeding is 11.8-11.9 g.

[0043] (3) The chicken bile extract taurochenodeoxycholic acid composition of the present invention has strong storage stability. After being placed in an environment with a temperature of 25°C and a relative humidity of 80% for 30 days, no moisture problem occurs;

[0044] (4) The chicken bile extract taurochenodeoxycholic acid composition of the present invention is easy to mix evenly with feed. After mixing 7 g of the chicken bile extract taurochenodeoxycholic acid composition of the present invention with 2 kg of Zhengda 900s Rutin chicken quail laying feed, stirring at room temperature at a stirring speed of 30 rpm for 10 minutes, the mixture can be evenly mixed. DETAILED DESCRIPTION

[0045] In order to have a clearer understanding of the technical features, purposes and effects of the present invention, the specific embodiments of the present invention are now described.

[0046] Example 1

[0047] A chicken bile extract taurochenodeoxycholic acid composition, comprising the following components by weight: 60 parts of a bile acid mixture and 40 parts of a composite additive;

[0048] The preparation method of the bile acid mixture is:

[0049] (1) Bile acid adsorption: Bile acid and anhydrous ethanol were mixed, stirred at room temperature at a stirring speed of 100 rpm for 2 h, light calcium carbonate was added, stirred for 24 h, filtered, and the filter residue was collected and dried to obtain calcium carbonate adsorbed with bile acid;

[0050] The mass ratio of bile acid, anhydrous ethanol, and light calcium carbonate is 23:1000:85;

[0051] The mass content of taurochenodeoxycholic acid in the bile acid is 6%;

[0052] The particle size of the light calcium carbonate is 80 μm;

[0053] (2) Adsorption of curcumin: Curcumin and anhydrous ethanol were mixed, stirred at room temperature at a stirring speed of 100 rpm for 4 h, calcium carbonate adsorbed with bile acid was added, stirred for 16 h, filtered, and the filter residue was collected and dried to obtain calcium carbonate adsorbed with curcumin;

[0054] The mass ratio of curcumin, anhydrous ethanol, and calcium carbonate for adsorbing bile acid is 13:3200:105;

[0055] (3) Preparing a coating solution: mixing hydropropyl methylcellulose, polyethylene glycol 6000, sodium carboxymethyl cellulose, magnesium stearate, calcium chloride, anhydrous ethanol, and deionized water, and stirring at room temperature at a stirring speed of 300 rpm for 40 minutes to obtain a coating solution;

[0056] The mass ratio of hypromellose, polyethylene glycol 400, sodium carboxymethyl cellulose, magnesium stearate, calcium chloride, anhydrous ethanol, and deionized water is 30:12:2:10:1:70:255;

[0057] The particle size of the magnesium stearate is 5 μm;

[0058] (4) Coating: Add the curcumin-adsorbed calcium carbonate into a coating machine and coat with a coating solution. The flow rate of the coating solution is controlled at 0.7 L / min, the inlet air temperature is 45°C, and the outlet air temperature is room temperature to obtain a bile acid mixture.

[0059] The mass ratio of calcium carbonate adsorbing curcumin to the coating solution was 90:27;

[0060] The preparation method of the composite additive is:

[0061] (1) Adsorption of water-soluble additives: Vitamin C, vitamin B1, vitamin B2, vitamin B5, γ-aminobutyric acid, and deionized water were mixed, stirred at room temperature at a stirring speed of 100 rpm for 40 minutes, light calcium carbonate was added, stirred for 9 hours, filtered, and the filter residue was collected and dried to obtain calcium carbonate for adsorption of water-soluble additives;

[0062] The mass ratio of vitamin C, vitamin B1, vitamin B2, vitamin B5, γ-aminobutyric acid, deionized water, and light calcium carbonate is 2:1:1:2:47:2000:180;

[0063] The particle size of the light calcium carbonate is 80 μm;

[0064] (2) Adsorption of soy protein: Soy protein and deionized water were mixed, stirred at a stirring speed of 200 rpm at room temperature for 3 h, and at a stirring speed of 100 rpm at 4°C for 7 h, centrifuged, and the supernatant was collected. The supernatant was mixed with calcium carbonate adsorbed with water-soluble additives, and stirred at a stirring speed of 100 rpm at room temperature for 17 h. The supernatant was filtered, and the filter residue was collected and dried to obtain calcium carbonate adsorbed with soy protein;

[0065] The mass ratio of soy protein, deionized water, and calcium carbonate adsorbed with water-soluble additives is 40:2300:230;

[0066] (3) Adsorption of fat-soluble additives: Vitamin A, vitamin D, vitamin E, curcumin, and anhydrous ethanol were mixed and stirred at 200 rpm for 4 h at room temperature. Calcium carbonate adsorbed with soy protein was added and stirred for 17 h. The mixture was filtered and the residue was dried to obtain calcium carbonate adsorbed with fat-soluble additives.

[0067] The mass ratio of vitamin A, vitamin D, vitamin E, curcumin, anhydrous ethanol, and calcium carbonate adsorbed with soy protein is 0.5:1:3:10:3000:255;

[0068] (4) Preparation of coating solution: Mix magnesium stearate, L-leucine, and the first portion of deionized water, stir at room temperature at a stirring speed of 200 rpm for 3 h, filter, collect the filter residue, mix the filter residue with hydropropyl methylcellulose, polyethylene glycol 6000, calcium chloride, anhydrous ethanol, and the second portion of deionized water, and stir at room temperature at a stirring speed of 300 rpm for 40 min to obtain a coating solution;

[0069] The mass ratio of magnesium stearate, L-leucine, the first portion of deionized water, hypromellose, polyethylene glycol 6000, calcium chloride, anhydrous ethanol, and the second portion of deionized water is 10:1.5:200:35:10:1:70:250;

[0070] The particle size of the magnesium stearate is 5 μm;

[0071] (5) Coating: Add the calcium carbonate adsorbed with the fat-soluble additive into the coating machine, and coat with the coating liquid. Control the flow rate of the coating liquid to be 0.6 L / min, the inlet air temperature to be 45°C, and the outlet air temperature to be room temperature to obtain the composite additive.

[0072] The mass ratio of calcium carbonate for adsorbing fat-soluble additives to the coating solution is 90:30.

[0073] A method for preparing the aforementioned chicken bile extract taurochenodeoxycholic acid composition comprises the following steps: mixing a bile acid mixture and a composite additive in prescribed parts by weight, and stirring the mixture at room temperature at a stirring speed of 30 rpm for 30 minutes to obtain the chicken bile extract taurochenodeoxycholic acid composition.

[0074] This embodiment also provides a use of the aforementioned chicken bile extract taurochenodeoxycholic acid composition in the preparation of an anti-stress drug for improving the laying period of rutin chickens.

[0075] Example 2

[0076] A chicken bile extract taurochenodeoxycholic acid composition, comprising the following components by weight: 62 parts of a bile acid mixture and 38 parts of a composite additive;

[0077] The preparation method of the bile acid mixture is:

[0078] (1) Bile acid adsorption: Bile acid and anhydrous ethanol were mixed, stirred at room temperature at a stirring speed of 120 rpm for 2.5 h, light calcium carbonate was added, stirred for 25 h, filtered, and the filter residue was collected and dried to obtain calcium carbonate adsorbed with bile acid;

[0079] The mass ratio of bile acid, anhydrous ethanol, and light calcium carbonate is 24:1050:88;

[0080] The mass content of taurochenodeoxycholic acid in the bile acid is 6%;

[0081] The particle size of the light calcium carbonate is 80 μm;

[0082] (2) Adsorption of curcumin: Curcumin and anhydrous ethanol were mixed and stirred at room temperature at a stirring speed of 120 rpm for 4-5 hours, and calcium carbonate adsorbed with bile acid was added and stirred for 17 hours. The mixture was filtered and the residue was collected and dried to obtain calcium carbonate adsorbed with curcumin.

[0083] The mass ratio of curcumin, anhydrous ethanol, and calcium carbonate for adsorbing bile acid is 14:3300:108;

[0084] (3) Preparation of coating solution: Hypromellose, polyethylene glycol 6000, sodium carboxymethyl cellulose, magnesium stearate, calcium chloride, anhydrous ethanol, and deionized water were mixed, and stirred at room temperature at a stirring speed of 350 rpm for 45 min to obtain a coating solution;

[0085] The mass ratio of hypromellose, polyethylene glycol 400, sodium carboxymethyl cellulose, magnesium stearate, calcium chloride, anhydrous ethanol, and deionized water is 32:13:2.2:12:1.1:72:265;

[0086] The particle size of the magnesium stearate is 5 μm;

[0087] (4) Coating: Add the curcumin-adsorbed calcium carbonate into a coating machine and coat with a coating solution. Control the flow rate of the coating solution to 0.7 L / min, the inlet air temperature to 48°C, and the outlet air temperature to room temperature to obtain a bile acid mixture.

[0088] The mass ratio of calcium carbonate adsorbing curcumin to the coating solution was 90:28;

[0089] The preparation method of the composite additive is:

[0090] (1) Adsorption of water-soluble additives: Vitamin C, vitamin B1, vitamin B2, vitamin B5, γ-aminobutyric acid, and deionized water were mixed, stirred at room temperature at a stirring speed of 120 rpm for 45 min, light calcium carbonate was added, stirred for 9.5 h, filtered, and the filter residue was collected and dried to obtain calcium carbonate for adsorption of water-soluble additives;

[0091] The mass ratio of vitamin C, vitamin B1, vitamin B2, vitamin B5, γ-aminobutyric acid, deionized water, and light calcium carbonate is 2.5:1.1:1.1:2.2:48:2200:190;

[0092] The particle size of the light calcium carbonate is 80 μm;

[0093] (2) Adsorption of soy protein: Soy protein and deionized water were mixed, stirred at a stirring speed of 300 rpm at room temperature for 3.5 h, and at a stirring speed of 150 rpm at 4°C for 7.5 h, centrifuged, and the supernatant was collected. The supernatant was mixed with calcium carbonate adsorbed with water-soluble additives, and stirred at a stirring speed of 120 rpm at room temperature for 18 h. The mixture was filtered, and the filter residue was collected and dried to obtain calcium carbonate adsorbed with soy protein.

[0094] The mass ratio of soy protein, deionized water, and calcium carbonate adsorbed with water-soluble additives is 41:2400:235;

[0095] (3) Adsorption of fat-soluble additives: Vitamin A, vitamin D, vitamin E, curcumin, and anhydrous ethanol were mixed and stirred at room temperature at a stirring speed of 250 rpm for 4.5 h. Calcium carbonate adsorbed with soy protein was added and stirred for 18 h. The mixture was filtered and the filter residue was dried to obtain calcium carbonate adsorbed with fat-soluble additives.

[0096] The mass ratio of vitamin A, vitamin D, vitamin E, curcumin, anhydrous ethanol, and calcium carbonate adsorbed with soy protein is 0.5:1.2:3.2:10.5:3100:265;

[0097] (4) Preparation of coating solution: Mix magnesium stearate, L-leucine, and the first portion of deionized water, stir at room temperature at a stirring speed of 250 rpm for 3.5 h, filter, take the filter residue, mix the filter residue with hydropropyl methylcellulose, polyethylene glycol 6000, calcium chloride, anhydrous ethanol, and the second portion of deionized water, and stir at room temperature at a stirring speed of 350 rpm for 45 min to obtain a coating solution;

[0098] The mass ratio of magnesium stearate, L-leucine, the first portion of deionized water, hypromellose, polyethylene glycol 6000, calcium chloride, anhydrous ethanol, and the second portion of deionized water is 12:1.8:220:36:12:1.1:72:260;

[0099] The particle size of the magnesium stearate is 5 μm;

[0100] (5) Coating: Add the calcium carbonate adsorbed with the fat-soluble additive into a coating machine, and coat with a coating solution. Control the flow rate of the coating solution to 0.6 L / min, the inlet air temperature to 48°C, and the outlet air temperature to room temperature to obtain a composite additive.

[0101] The mass ratio of calcium carbonate for adsorbing fat-soluble additives to the coating solution is 90:31.

[0102] A method for preparing the aforementioned chicken bile extract taurochenodeoxycholic acid composition comprises the following steps: mixing a bile acid mixture and a composite additive in prescribed parts by weight, and stirring the mixture at a stirring speed of 40 rpm for 35 minutes at room temperature to obtain the chicken bile extract taurochenodeoxycholic acid composition.

[0103] This embodiment also provides a use of the aforementioned chicken bile extract taurochenodeoxycholic acid composition in the preparation of an anti-stress drug for improving the laying period of rutin chickens.

[0104] Example 3

[0105] A chicken bile extract taurochenodeoxycholic acid composition, comprising the following components by weight: 65 parts of a bile acid mixture and 40 parts of a composite additive;

[0106] The preparation method of the bile acid mixture is:

[0107] (1) Bile acid adsorption: Bile acid and anhydrous ethanol were mixed, stirred at room temperature at a stirring speed of 150 rpm for 3 h, light calcium carbonate was added, stirred for 26 h, filtered, and the filter residue was collected and dried to obtain calcium carbonate adsorbed with bile acid;

[0108] The mass ratio of bile acid, anhydrous ethanol, and light calcium carbonate is 25:1100:90;

[0109] The mass content of taurochenodeoxycholic acid in the bile acid is 6%;

[0110] The particle size of the light calcium carbonate is 80 μm;

[0111] (2) Adsorption of curcumin: Curcumin and anhydrous ethanol were mixed and stirred at room temperature at a stirring speed of 150 rpm for 5 h, calcium carbonate adsorbed with bile acid was added, and stirred for 18 h. The mixture was filtered, and the filter residue was collected and dried to obtain calcium carbonate adsorbed with curcumin;

[0112] The mass ratio of curcumin, anhydrous ethanol, and calcium carbonate for adsorbing bile acid is 15:3500:110;

[0113] (3) Preparing a coating solution: mixing hydropropyl methylcellulose, polyethylene glycol 6000, sodium carboxymethyl cellulose, magnesium stearate, calcium chloride, anhydrous ethanol, and deionized water, and stirring at room temperature at a stirring speed of 400 rpm for 50 min to obtain a coating solution;

[0114] The mass ratio of hypromellose, polyethylene glycol 400, sodium carboxymethyl cellulose, magnesium stearate, calcium chloride, anhydrous ethanol, and deionized water is 35:15:2.5:14:1.2:75:270;

[0115] The particle size of the magnesium stearate is 5 μm;

[0116] (4) Coating: Add the curcumin-adsorbed calcium carbonate into a coating machine and coat with a coating solution. Control the flow rate of the coating solution to 0.8 L / min, the inlet air temperature to 50°C, and the outlet air temperature to room temperature to obtain a bile acid mixture.

[0117] The mass ratio of calcium carbonate adsorbed with curcumin to the coating solution was 90:29;

[0118] The preparation method of the composite additive is:

[0119] (1) Adsorption of water-soluble additives: Mix vitamin C, vitamin B1, vitamin B2, vitamin B5, γ-aminobutyric acid, and deionized water, stir at room temperature at a stirring speed of 150 rpm for 50 minutes, add light calcium carbonate, stir for 10 hours, filter, take the filter residue, and dry the filter residue to obtain calcium carbonate for adsorption of water-soluble additives;

[0120] The mass ratio of vitamin C, vitamin B1, vitamin B2, vitamin B5, γ-aminobutyric acid, deionized water, and light calcium carbonate is 3:1.3:1.3:2.5:50:2400:200;

[0121] The particle size of the light calcium carbonate is 80 μm;

[0122] (2) Adsorption of soy protein: Soy protein and deionized water were mixed, stirred at a stirring speed of 400 rpm at room temperature for 4 h, and at a stirring speed of 200 rpm at 5°C for 8 h, centrifuged, and the supernatant was collected. The supernatant was mixed with calcium carbonate adsorbed with water-soluble additives, and stirred at a stirring speed of 150 rpm at room temperature for 20 h. The mixture was filtered, and the filter residue was collected and dried to obtain calcium carbonate adsorbed with soy protein.

[0123] The mass ratio of soy protein, deionized water, and calcium carbonate adsorbed with water-soluble additives is 42:2500:240;

[0124] (3) Adsorption of fat-soluble additives: Vitamin A, vitamin D, vitamin E, curcumin, and anhydrous ethanol were mixed and stirred at 300 rpm at room temperature for 5 h. Calcium carbonate adsorbed with soy protein was added and stirred for 20 h. The mixture was filtered and the residue was dried to obtain calcium carbonate adsorbed with fat-soluble additives.

[0125] The mass ratio of vitamin A, vitamin D, vitamin E, curcumin, anhydrous ethanol, and calcium carbonate adsorbed with soy protein is 0.6:1.3:3.5:11:3200:270;

[0126] (4) Preparation of coating solution: Mix magnesium stearate, L-leucine, and the first portion of deionized water, stir at room temperature at a stirring speed of 300 rpm for 4 h, filter, collect the filter residue, mix the filter residue with hydropropyl methylcellulose, polyethylene glycol 6000, calcium chloride, anhydrous ethanol, and the second portion of deionized water, and stir at room temperature at a stirring speed of 400 rpm for 50 min to obtain a coating solution;

[0127] The mass ratio of magnesium stearate, L-leucine, the first portion of deionized water, hypromellose, polyethylene glycol 6000, calcium chloride, anhydrous ethanol, and the second portion of deionized water is 14:2:230:38:13:1.2:75:270;

[0128] The particle size of the magnesium stearate is 5 μm;

[0129] (5) Coating: Add the calcium carbonate adsorbed with the fat-soluble additive into a coating machine, and coat with a coating solution. Control the flow rate of the coating solution to 0.7 L / min, the inlet air temperature to 50°C, and the outlet air temperature to room temperature to obtain a composite additive.

[0130] The mass ratio of calcium carbonate for adsorbing fat-soluble additives to the coating solution is 90:32.

[0131] A method for preparing the aforementioned chicken bile extract taurochenodeoxycholic acid composition comprises the following steps: mixing a bile acid mixture and a composite additive in prescribed parts by weight, and stirring the mixture at a stirring speed of 50 rpm at room temperature for 40 minutes to obtain the chicken bile extract taurochenodeoxycholic acid composition.

[0132] This embodiment also provides a use of the aforementioned chicken bile extract taurochenodeoxycholic acid composition in the preparation of an anti-stress drug for improving the laying period of rutin chickens.

[0133] Comparative Example 1

[0134] Based on Example 1, adjustments were made. The specific adjustments were as follows: in the preparation of the bile acid mixture, the curcumin adsorption step (2) was omitted; in the preparation of the coating solution step (3), the addition of sodium carboxymethyl cellulose was omitted; and in the preparation of the composite additive, the addition of curcumin was omitted in the adsorption step (3) of the fat-soluble additive.

[0135] The rest is the same as Example 1.

[0136] Comparative Example 2

[0137] On the basis of Example 1, adjustments were made. Specifically, in the preparation of the composite additive, the soybean protein adsorption step (2) was omitted; and in the preparation of the coating solution step (4), the addition of L-leucine was omitted. Specifically, the preparation of the coating solution step (4) was changed to:

[0138] Mix magnesium stearate, hypromellose, polyethylene glycol 6000, calcium chloride, anhydrous ethanol, and a second portion of deionized water, and stir at room temperature at a stirring speed of 300 rpm for 40 minutes to obtain a coating solution;

[0139] The mass ratio of magnesium stearate, hypromellose, polyethylene glycol 6000, calcium chloride, anhydrous ethanol, and the second portion of deionized water is 10:35:10:1:70:250;

[0140] The particle size of the magnesium stearate is 5 μm.

[0141] The rest is the same as Example 1.

[0142] Test Example 1

[0143] 360 115-120-day-old rudin chickens at the peak of egg-laying period were selected from a rudin chicken farm in Weifang City, Shandong Province. Among them, 120 male rudin chickens and 240 female rudin chickens were randomly divided into 6 groups, respectively labeled as Group 1, Group 2, Group 3, Group 4, Group 5, and Group 6, with 60 chickens in each group, ensuring that each group had 20 male rudin chickens and 40 female rudin chickens, and the male and female rudin chickens in each group were numbered to facilitate differentiation;

[0144] Groups of 1-6 roosters were housed in the same henhouse, with one male and two females per cage. All roosters within the same cage were kept from the same group. The temperature in the roosterhouse was maintained at 25°C and the relative humidity at 65%. The roosters were fed ad libitum with drinking water that met the GB 5749-2022 standard for drinking water hygiene. The feed used was Zhengda 900s rooster and quail laying feed.

[0145] The chicken bile extract taurochenodeoxycholic acid compositions of Examples 1-3 and Comparative Examples 1-2 were added to the feed of Groups 1-5, respectively, and stirred evenly. The addition amount was 3.5 g / kg for each group. Group 6 served as a blank control, without adding any feed additives.

[0146] The broilers were fed for 5 consecutive days, with the first day being a normal feeding day. After the first day, the fecal tray in the cage of each broiler was cleaned once, and eggs were picked up once in the cage of each female broiler. The total egg production, number of laying hens, total feed intake, and total water intake of each group of broilers on the first day were recorded, and the egg production rate, average daily feed intake, and average daily water intake of each broiler were calculated. At 8 a.m. on the second day of feeding, an artificial stressor was set up, specifically, each broiler was grabbed once every two hours, the fecal tray in the cage of each broiler was cleaned once every two hours, and eggs were picked up once in the simulated cage of each female broiler every two hours. After 5 p.m., no artificial stressor was set up, and the egg production, feed intake, and water drinking data for the second day were not recorded.

[0147] The third, fourth, and fifth days of feeding were normal feeding. At the end of each day, the fecal tray in each hen's experimental cage was cleaned once, and eggs were collected once in each hen's experimental cage. The total egg production, number of laying hens, total feed intake, and total water intake of each group of hen were recorded. The egg production rate, average daily feed intake per hen, and average daily water intake per hen were calculated for each group.

[0148] Among them, egg production rate = number of laying hens / 40 × 100%;

[0149] The records and calculation results are as follows:

[0150]

[0151] Test Example 2

[0152] 100 g of the chicken bile extract taurochenodeoxycholic acid composition of Examples 1-3 and Comparative Examples 1-2 were respectively taken and placed in a 100 mL beaker. After being left open for 30 days in an environment at a temperature of 25 ° C. and a relative humidity of 80%, the chicken bile extract taurochenodeoxycholic acid composition was observed for moisture problems, specifically, whether the surface of the chicken bile extract taurochenodeoxycholic acid composition was wet and sticky. The observation results are as follows:

[0153]

[0154] Test Example 3

[0155] 7 g of the chicken bile extract taurochenodeoxycholic acid composition of Examples 1-3 and Comparative Examples 1-2 were respectively mixed with 2 kg of Zhengda 900s rutin chicken and quail laying feed, and stirred at room temperature at a stirring speed of 30 rpm for 10 minutes to observe whether the mixture was uniform. The observation results are as follows:

[0156]

[0157] From the above results, it can be seen that in Comparative Example 1, the curcumin adsorption step was omitted in the preparation of the bile acid mixture, the addition of sodium carboxymethyl cellulose was omitted in the preparation of the coating solution, and the addition of curcumin was omitted in the adsorption of the fat-soluble additive in the preparation of the composite additive. As a result, the chicken bile extract taurochenodeoxycholic acid composition of Comparative Example 1 had poor anti-stress ability and ability to increase feed intake in rutin chickens during the laying period, and poor storage stability;

[0158] In the preparation of the composite additive in Comparative Example 2, the soy protein adsorption step was omitted, and the addition of L-leucine was omitted in the coating solution preparation step. As a result, the chicken bile extract taurochenodeoxycholic acid composition of Comparative Example 2 had poor anti-stress ability and feed intake improvement ability for rutin chickens during the laying period, good storage stability, and was not easy to mix evenly with the feed.

[0159] The invention relates to a method for preparing a bile acid mixture, wherein a curcumin adsorption step is added, specifically, curcumin is added to the calcium carbonate adsorbing bile acid. Curcumin can be adsorbed on the surface of the calcium carbonate adsorbing bile acid, thereby achieving the following effects: first, bile acid can be encapsulated and fixed to prevent bile acid loss; second, bile acid can be encapsulated before coating, thereby protecting bile acid and preventing water vapor from penetrating the coating film and affecting bile acid during long-term storage; third, curcumin acts as a flavoring agent, thereby increasing the feed intake of roe deer, while avoiding the problem of flavoring agent being easily ineffective when directly added to the coating solution; and fourth, during the digestion of roe deer, curcumin can promote the absorption of bile acid by roe deer.

[0160] Curcumin is added to the adsorbable fat-soluble additive in the preparation of the composite additive, thereby achieving the following effects: first, curcumin acts as a flavoring agent, which can increase the feed intake of rutin chickens, while avoiding the problem of flavoring agents being easily ineffective when directly added to the coating solution; second, curcumin can adsorb other fat-soluble additives and promote contact with bile acids.

[0161] In the preparation of the composite additive, light calcium carbonate is first used to adsorb the water-soluble additive, and the water-soluble additive is adsorbed on the surface of the light calcium carbonate. Then, soybean protein is adsorbed. The soybean protein contains hydrophilic groups and hydrophobic groups. The hydrophilic groups and the water-soluble additives generate intermolecular forces and are combined with the surface of the water-soluble additive. Then, the fat-soluble additive is adsorbed. The fat-soluble additive and the hydrophobic groups of the soybean groups generate intermolecular forces and are combined with the surface of the soybean protein. Finally, coating is performed, thereby achieving the following effects: First, the adsorption force and adsorption amount of the fat-soluble additive are improved, the coating difficulty is reduced, the coating efficiency is improved, and thus the composite additive is improved. Secondly, during the digestion of the rooster, the bile acid in the bile acid mixture is digested first. Due to the difference in the composition of the coating solution for the bile acid mixture and the composite additive, the composite additive disintegrates more slowly than the bile acid mixture. After the bile acid is released, the fat-soluble additive in the composite additive is released, and bile acid can promote the absorption of the fat-soluble additive. Thirdly, soy protein can slow the release of water-soluble additives. Most of the water-soluble additives are released in the small intestine of the rooster, avoiding excessively fast release that may cause interaction with bile acid.

[0162] In the coating step of the preparation of the composite additive, the magnesium stearate added to the coating solution is magnesium stearate pretreated with L-leucine, thereby achieving the following effects: first, the hydrophilicity and dispersibility of the magnesium stearate are improved, and the toughness and stability of the coating film are improved; second, after disintegration, the pretreated magnesium stearate can be combined with soy protein, and further, through the combination of soy protein and water-soluble additives, the pretreated magnesium stearate can be adsorbed in the small intestine of rutin chickens, thereby prolonging the digestion time of the water-soluble additive and improving the utilization rate of the water-soluble additive.

[0163] Unless otherwise specified, all percentages used in the present invention are by mass.

[0164] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art will be able to modify the technical solutions described in the aforementioned embodiments or substitute equivalents for some of the technical features. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A chicken bile extract taurochenodeoxycholic acid composition, characterized in that: The composition is composed of the following components by weight: 60-65 parts of bile acid mixture, 35-40 parts of composite additives; The method for preparing the bile acid mixture comprises the following steps: adsorbing bile acid, adsorbing curcumin, preparing a coating solution, and coating; The bile acid adsorption comprises mixing bile acid and anhydrous ethanol, stirring at room temperature, adding light calcium carbonate, stirring, filtering, taking filter residue, and drying the filter residue to obtain calcium carbonate adsorbing bile acid; The curcumin adsorption comprises mixing curcumin and anhydrous ethanol, stirring at room temperature, adding calcium carbonate adsorbing bile acid, stirring, filtering, taking filter residue, and drying the filter residue to obtain calcium carbonate adsorbing curcumin; In the method for preparing the bile acid mixture, a coating solution is prepared by mixing hydroxypropyl methylcellulose, polyethylene glycol 6000, sodium carboxymethyl cellulose, magnesium stearate, calcium chloride, anhydrous ethanol and deionized water, and stirring at room temperature to obtain a coating solution; The coating in the preparation method of the bile acid mixture comprises adding the calcium carbonate adsorbed with curcumin into a coating machine and coating with a coating liquid to obtain the bile acid mixture; The preparation method of the composite additive comprises the following steps: adsorbing water-soluble additives, adsorbing soybean protein, adsorbing fat-soluble additives, preparing coating liquid, and coating; The water-soluble additive adsorbing agent comprises mixing vitamin C, vitamin B1, vitamin B2, vitamin B5, γ-aminobutyric acid and deionized water, stirring at room temperature, adding light calcium carbonate, stirring, filtering, taking the filter residue, and drying the filter residue to obtain calcium carbonate adsorbing the water-soluble additive; The soy protein adsorption comprises mixing soy protein and deionized water, stirring at room temperature, stirring at 4-5°C, centrifuging, taking the supernatant, mixing the supernatant with calcium carbonate adsorbing water-soluble additives, stirring at room temperature, filtering, taking the filter residue, and drying the filter residue to obtain calcium carbonate adsorbing soy protein; The fat-soluble additive adsorption comprises mixing vitamin A, vitamin D, vitamin E, curcumin and anhydrous ethanol, stirring at room temperature, adding calcium carbonate adsorbing soy protein, stirring, filtering, taking the filter residue, and drying the filter residue to obtain calcium carbonate adsorbing the fat-soluble additive; In the method for preparing the composite additive, the coating solution is prepared by mixing magnesium stearate, L-leucine, and the first portion of deionized water, stirring at room temperature, filtering, taking the filter residue, mixing the filter residue with hydroxypropyl methylcellulose, polyethylene glycol 6000, calcium chloride, anhydrous ethanol, and the second portion of deionized water, and stirring at room temperature to obtain a coating solution; The coating in the preparation method of the composite additive is to add the calcium carbonate adsorbed with the fat-soluble additive into a coating machine and coat it with a coating liquid to obtain the composite additive; The preparation method of the chicken bile extract taurochenodeoxycholic acid composition comprises the following steps: mixing a bile acid mixture and a composite additive according to a specified weight ratio, and stirring the mixture at room temperature to obtain the chicken bile extract taurochenodeoxycholic acid composition.

2. The chicken bile extract taurochenodeoxycholic acid composition according to claim 1, characterized in that: In the adsorbed bile acid, the mass ratio of bile acid, anhydrous ethanol and light calcium carbonate is 23-25:1000-1100:85-90; The mass content of taurochenodeoxycholic acid in the bile acid is 6%.

3. The chicken bile extract taurochenodeoxycholic acid composition according to claim 1, characterized in that: In the adsorbed curcumin, the mass ratio of curcumin, anhydrous ethanol and calcium carbonate for adsorbing bile acid is 13-15:3200-3500:105-110.

4. The chicken bile extract taurochenodeoxycholic acid composition according to claim 1, characterized in that: In the preparation method of the bile acid mixture, in the coating solution, the mass ratio of hydroxypropyl methylcellulose, polyethylene glycol 400, sodium carboxymethyl cellulose, magnesium stearate, calcium chloride, anhydrous ethanol, and deionized water is 30-35:12-15:2-2.5:10-14:1-1.2:70-75:255-270; In the coating of the preparation method of the bile acid mixture, the mass ratio of calcium carbonate adsorbing curcumin to the coating liquid is 90:27-29; The flow rate of the coating liquid in the coating is 0.7-0.8 L / min, the air inlet temperature is 45-50° C., and the air outlet temperature is room temperature.

5. The chicken bile extract taurochenodeoxycholic acid composition according to claim 1, characterized in that: In the adsorbable water-soluble additive, the mass ratio of vitamin C, vitamin B1, vitamin B2, vitamin B5, γ-aminobutyric acid, deionized water and light calcium carbonate is 2-3:1-1.3:1-1.3:2-2.5:47-50:2000-2400:180-200.

6. The chicken bile extract taurochenodeoxycholic acid composition according to claim 1, characterized in that: In the adsorbed soybean protein, the mass ratio of soybean protein, deionized water and calcium carbonate for adsorbing water-soluble additives is 40-42:2300-2500:230-240.

7. The chicken bile extract taurochenodeoxycholic acid composition according to claim 1, characterized in that: In the adsorbed fat-soluble additive, the mass ratio of vitamin A, vitamin D, vitamin E, curcumin, anhydrous ethanol, and calcium carbonate adsorbed with soybean protein is 0.5-0.6:1-1.3:3-3.5:10-11:3000-3200:255-270.

8. The chicken bile extract taurochenodeoxycholic acid composition according to claim 1, characterized in that: In the coating solution prepared in the method for preparing the composite additive, the mass ratio of magnesium stearate, L-leucine, the first portion of deionized water, hydroxypropyl methylcellulose, polyethylene glycol 6000, calcium chloride, anhydrous ethanol, and the second portion of deionized water is 10-14:1.5-2:200-230:35-38:10-13:1-1.2:70-75:250-270; In the coating of the preparation method of the composite additive, the mass ratio of calcium carbonate adsorbing the fat-soluble additive to the coating liquid is 90:30-32; The flow rate of the coating liquid in the coating is 0.6-0.7 L / min, the air inlet temperature is 45-50° C., and the air outlet temperature is room temperature.

9. Use of the chicken bile extract taurochenodeoxycholic acid composition according to any one of claims 1 to 8 in the preparation of an anti-stress drug for improving the egg-laying period of rutin chickens.

Images