Bone scaffold composite material and preparation method thereof, bone scaffold

Abstract

The present invention relates to the technical field of bone scaffolds, and more specifically, to a bone scaffold composite material, a preparation method thereof, and a bone scaffold. The bone scaffold composite material prepared by the present invention has a layered structure, wherein the inner layer is a porous material loaded with a bone tissue repair-promoting drug, the middle layer is a polydopamine layer, and the outer layer is in situ grown Ag particles (antibacterial drugs). The material can be used to make a bone scaffold. In the prepared bone scaffold, the polydopamine layer plays a role in isolating the bone tissue repair-promoting drug and the antibacterial drug, thereby preventing the bone tissue repair-promoting drug and the antibacterial drug from reacting with each other and failing. Since the porous material loaded with the bone tissue repair-promoting drug is located in the inner layer, it can ensure that the bone tissue repair-promoting drug is released after the antibacterial drug is released. The bone scaffold provided by the present invention has an adjustable elastic modulus that can match the mechanical strength of human cancellous bone.

Description

Technical Field

[0001] The present invention relates to the technical field of bone scaffolds, and in particular to a bone scaffold composite material and a preparation method thereof, and a bone scaffold. Background Art

[0002] Bone is an integral part of the human body, maintaining bone mass and load-bearing properties through continuous self-repair. However, bone self-repair is subject to the extent of the defect and its physiological condition. Bone remodeling is a complex physiological process involving the interaction of multiple cell types and various physiological factors, requiring the synergistic effect of multiple parties to maintain the integrity of bone tissue. Although some drugs can relieve pain, improve function, and delay the course of the disease, and some scaffolds can prevent bone collapse, most of them have adverse reactions. Compared with traditional treatment methods, bone tissue engineering has become an effective strategy for treating bone defects.

[0003] When implanting an artificially designed bone scaffold, bone tissue engineering should also load bone tissue repair drugs (such as dexamethasone) on the bone scaffold. In addition, the bone scaffold also needs to carry an appropriate amount of antibacterial drugs to prevent infection at the implantation site in the human body. However, there is a possible chemical reaction between bone tissue repair drugs and antibacterial drugs, and different drugs act at different stages of bone repair. Therefore, carrying bone tissue repair drugs and antibacterial drugs on the bone scaffold may cause the drugs to react with each other and become ineffective. In addition, bone tissue repair drugs need to be released after the antibacterial drugs are released. Summary of the Invention

[0004] The technical problem solved by the present invention is how to prevent the bone tissue repair promoting drug and the antibacterial drug loaded on the bone scaffold from reacting with each other and becoming ineffective, and how to release the bone tissue repair promoting drug after the antibacterial drug is released.

[0005] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0006] A method for preparing a bone scaffold composite material, comprising:

[0007] Step S1, mixing porous calcium carbonate and eggshell powder in a mass ratio of 1: (0.9-1.1) to obtain a first mixed powder; ball-milling calcium hydroxyphosphate and calcium titanate in a mass ratio of 1: (0.9-1.1) to obtain a second mixed powder; mixing the first mixed powder and the second mixed powder in a mass ratio of (1.9-2.1): 1 to obtain a third mixed powder;

[0008] Step S2, adding the third mixed powder to water to obtain a first mixed solution; mixing the PBS solution containing the bone tissue repair promoting drug with the first mixed solution to obtain a second mixed solution;

[0009] Step S3, stirring the second mixed solution for the first time, centrifuging, collecting the precipitate, washing and drying to obtain nanoparticles loaded with the bone tissue repair promoting drug;

[0010] Step S4, adding the nanoparticles loaded with the bone tissue repair promoting drug to the dopamine solution to obtain a third mixed solution; stirring the third mixed solution for a second time, centrifuging, collecting the precipitate, washing and drying to obtain a PDA-modified powder loaded with the bone tissue repair promoting drug;

[0011] Step S5: adding the PDA-modified powder loaded with the bone tissue repair promoting drug into a silver nitrate solution, stirring for reaction, centrifuging, collecting the precipitate, washing and drying to obtain a bone scaffold composite material.

[0012] Preferably, in step S1, the method for preparing porous calcium carbonate includes:

[0013] Step S11, dissolving CaCl2 in NH4Cl solution to obtain a first solution; dissolving NH4HCO3 and H2O4 in NH4Cl solution to obtain a second solution;

[0014] Step S12: mixing the first solution and the second solution, stirring to react, filtering, and drying to obtain the porous calcium carbonate.

[0015] Preferably, in the first solution, the concentration of CaCl2 is 0.9-1.1 mol / L; in the second solution, the concentration of NH4HCO3 is 1.9-2.1 mol / L, and the concentration of H2O4 is 0.015-0.025 mol / L.

[0016] Preferably, in the second mixed solution, the concentration of the bone tissue repair promoting drug is 0.9-1.1 g / L, and the concentration of the third mixed powder is 2-3 g / L.

[0017] Preferably, in the third mixed solution, the concentration of the nanoparticles loaded with the bone tissue repair promoting drug is 2-3 g / L, and the concentration of dopamine is 2-3 g / L.

[0018] Preferably, the bone tissue repair promoting drug includes one of dexamethasone, Kartogenin, cucurbitacin B and bone morphogenetic protein.

[0019] The present invention also provides a bone scaffold composite material, which is prepared by the above-mentioned preparation method of the bone scaffold composite material.

[0020] Compared with the prior art, the bone scaffold composite material prepared by the method of the present invention has a layered structure, wherein the inner layer is a porous material loaded with a bone tissue repair-promoting drug, the middle layer is a polydopamine layer, and the outer layer is in situ grown Ag particles (antibacterial drugs). This material can be used to make bone scaffolds. In the prepared bone scaffold, the polydopamine layer plays a role in isolating the bone tissue repair-promoting drug and the antibacterial drug, thereby preventing the bone tissue repair-promoting drug and the antibacterial drug from reacting with each other and becoming ineffective. Since the porous material loaded with the bone tissue repair-promoting drug is located in the inner layer, it can ensure that the bone tissue repair-promoting drug is released after the antibacterial drug (Ag particles) is released. In addition, since the porous material loaded with bone tissue repair promoting drugs contains porous calcium carbonate, porous calcium carbonate has a large specific surface area, which is conducive to the adsorption and loading of bone tissue repair promoting drugs; since the porous material loaded with bone tissue repair promoting drugs contains eggshell powder, the components contained in the eggshell powder are close to bone tissue, which is more conducive to the recovery of bone tissue; since the porous material loaded with bone tissue repair promoting drugs contains hydroxy calcium phosphate and calcium titanate, calcium hydroxy phosphate is electrically active and can sensitively sense biocurrent stimulation, thereby promoting bone tissue regeneration, and calcium titanate can serve as a conductive phase to enhance the ability of hydroxy calcium phosphate to sense biocurrent stimulation, thereby further promoting bone tissue regeneration.

[0021] The present invention also provides a bone scaffold, which is made of a mixed material through 4D printing, and the mixed material is made by mixing the bone scaffold composite material as described above and a degradable shape memory material.

[0022] Compared with the prior art, the bone scaffold provided by the present invention has the above advantages. Since the material composition of the bone scaffold contains shape memory material, the bone scaffold has shape memory properties. The shape of the bone scaffold can be changed by external stimulation (such as thermal stimulation), which is conducive to the minimally invasive implantation of the bone scaffold.

[0023] Preferably, the bone scaffold is composed of a bone scaffold unit, which includes a CaF2 unit cell structure framework and a quadratic curve structure. The quadratic curve structure includes a quadratic curve body located on each bottom surface of the CaF2 unit cell structure framework. The CaF2 unit cell structure framework includes a cubic frame composed of hollow straight rods, and a hollow short rod is provided on each of the eight vertices of the cubic frame. The quadratic curve body is composed of hollow curved rods, the inner diameter of the hollow straight rod is 300-600 μm, and the aperture size of the hollow curved rod is 300-600 μm.

[0024] Preferably, the quadratic curve body is a structure composed of hollow curved rods and having a shape similar to a quadratic curve, and the quadratic curve includes one of an Archimedean spiral, a hyperbola, a parabola, a first function curve, a second function curve, and a third function curve; the first function curve satisfies the following functional relationship:

[0025] y=(0.09(5sin0.5x-2.5sinx) 2 -1) 4 ;

[0026] The second function curve satisfies the following functional relationship:

[0027] y=(0.09(5sin0.5x-2.5sinx) 2 -1.05) 4 ;

[0028] The third function curve satisfies the following functional relationship:

[0029] y=(0.08(5sin0.5x-2.5sinx) 2 -1) 4 ;

[0030] Among them, x is the horizontal coordinate and y is the vertical coordinate. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 Schematic diagram of the process for preparing the bone scaffold composite material according to an embodiment of the present invention;

[0032] Figure 2 Schematic diagram of the structure of the bone scaffold unit in an embodiment of the present invention;

[0033] Figure 3 A schematic diagram of the structure of the CaF2 unit cell structure framework in the embodiment of the invention;

[0034] Figure 4 This is one of the structural diagrams of a quadratic curve body in an embodiment of the invention;

[0035] Figure 5 This is the second structural diagram of the quadratic curve body in the embodiment of the invention.

[0036] Description of reference numerals:

[0037] 1. Imitation CaF2 unit cell structure framework, 2. Quadratic curve body. DETAILED DESCRIPTION

[0038] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, specific embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0039] It should be noted that, unless otherwise specified, the features of the embodiments of the present invention may be combined with each other. The terms "comprising," "including," "containing," and "having" are non-restrictive and may include other steps and other components that do not affect the results. The above terms encompass the terms "consisting of" and "consisting essentially of." Unless otherwise specified, materials, equipment, and reagents were commercially available.

[0040] like Figure 1 As shown, an embodiment of the present invention provides a method for preparing a bone scaffold composite material, comprising:

[0041] Step S1, mixing porous calcium carbonate and eggshell powder in a mass ratio of 1: (0.9-1.1) to obtain a first mixed powder; ball-milling calcium hydroxyphosphate and calcium titanate in a mass ratio of 1: (0.9-1.1) to obtain a second mixed powder; mixing the first mixed powder and the second mixed powder in a mass ratio of (1.9-2.1): 1 to obtain a third mixed powder;

[0042] Step S2, adding the third mixed powder to water to obtain a first mixed solution; mixing a PBS solution containing a bone tissue repair promoting drug with the first mixed solution to obtain a second mixed solution; the bone tissue repair promoting drug comprises one of dexamethasone, Kartogenin, cucurbitacin B, and bone morphogenetic protein;

[0043] Step S3, stirring the second mixed solution for the first time, centrifuging, collecting the precipitate, washing and drying to obtain nanoparticles loaded with the bone tissue repair promoting drug;

[0044] Step S4, adding the nanoparticles loaded with the bone tissue repair promoting drug to the dopamine solution to obtain a third mixed solution; stirring the third mixed solution for a second time, centrifuging, collecting the precipitate, washing and drying to obtain a PDA-modified powder loaded with the bone tissue repair promoting drug;

[0045] Step S5: adding the PDA-modified powder loaded with a bone tissue repair-promoting drug to a silver nitrate solution, stirring the reaction, centrifuging, collecting the precipitate, washing and drying to obtain a bone scaffold composite material, wherein the inner layer of the bone scaffold composite material is a porous material loaded with a bone tissue repair-promoting drug, the middle layer is a polydopamine layer, and the outer layer is in situ grown Ag particles (antibacterial drugs).

[0046] The bone repair process generally proceeds through three phases: a hematoma-inflammation phase 2-4 weeks after bone grafting, a primary callus formation phase 4-8 weeks, and a bone plate remodeling phase 8-14 weeks. The outer layer of Ag particles releases 60-70% of its content within one week of implantation, followed by a steady silver ion release rate of 0.05-0.15% / day over the next two weeks, effectively addressing bacterial infection during implantation. The polydopamine layer begins to release after being exposed, eliminating reactive oxygen species and shortening the inflammatory cycle. Initially after implantation, the PDA layer protects the bone tissue repair drug from release into the body. After 5-7 days, 10-15% of the drug is released from the porous material, followed by a continuous release of 70-75% over the next 3-5 weeks, promoting the differentiation of mesenchymal stem cells into callus.

[0047] Compared with the prior art, the bone scaffold composite material prepared by the method of the embodiment of the present invention has a layered structure, wherein the inner layer is a porous material loaded with a bone tissue repair-promoting drug, the middle layer is a polydopamine layer, and the outer layer is in situ grown Ag particles (antibacterial drugs). This material can be used to make bone scaffolds. In the prepared bone scaffold, the polydopamine layer plays a role in isolating the bone tissue repair-promoting drug and the antibacterial drug, thereby preventing the bone tissue repair-promoting drug and the antibacterial drug from reacting with each other and failing. Since the porous material loaded with the bone tissue repair-promoting drug is located in the inner layer, it can ensure that the bone tissue repair-promoting drug is released after the antibacterial drug (Ag particles) is released. In addition, since the porous material loaded with bone tissue repair promoting drugs contains porous calcium carbonate, porous calcium carbonate has a large specific surface area, which is conducive to the adsorption and loading of bone tissue repair promoting drugs; since the porous material loaded with bone tissue repair promoting drugs contains eggshell powder, the components contained in the eggshell powder are close to bone tissue, which is more conducive to the recovery of bone tissue; since the porous material loaded with bone tissue repair promoting drugs contains hydroxy calcium phosphate and calcium titanate, calcium hydroxy phosphate is electrically active and can sensitively sense biocurrent stimulation, thereby promoting bone tissue regeneration, and calcium titanate can serve as a conductive phase to enhance the ability of hydroxy calcium phosphate to sense biocurrent stimulation, thereby further promoting bone tissue regeneration.

[0048] In some embodiments of the present invention, in step S1, the method for preparing porous calcium carbonate includes:

[0049] Step S11, dissolving CaCl2 in NH4Cl solution to obtain a first solution; dissolving NH4HCO3 and H2O4 in NH4Cl solution to obtain a second solution;

[0050] Step S12: mixing the first solution and the second solution, stirring to react, filtering, and drying to obtain the porous calcium carbonate.

[0051] In some embodiments of the present invention, in the first solution, the concentration of CaCl2 is 0.9-1.1 mol / L; in the second solution, the concentration of NH4HCO3 is 1.9-2.1 mol / L, and the concentration of H2O4 is 0.015-0.025 mol / L.

[0052] In some embodiments of the present invention, in the second mixed solution, the concentration of the bone tissue repair promoting drug is 1.9-2.1 g / L, and the concentration of the third mixed powder is 4.5-5.5 g / L.

[0053] In some embodiments of the present invention, in the third mixed solution, the concentration of the nanoparticles loaded with the bone tissue repair promoting drug is 2-3 g / L, and the concentration of dopamine is 2-3 g / L.

[0054] In some embodiments of the present invention, the first stirring treatment lasts for 10-14 hours. The first stirring treatment lasts for 10-14 hours.

[0055] The present invention also provides a bone scaffold composite material, which is prepared by the above-mentioned preparation method of the bone scaffold composite material.

[0056] The present invention also provides a bone scaffold, which is made of a mixed material through 4D printing, and the mixed material is made by mixing the bone scaffold composite material as described above and a shape memory material.

[0057] Compared with the prior art, the bone scaffold provided by the present invention has the above advantages. Since the material composition of the bone scaffold contains degradable shape memory material, the bone scaffold has shape memory properties. The shape of the bone scaffold can be changed by external stimulation (such as thermal stimulation), which is conducive to the minimally invasive implantation of the bone scaffold. In addition, the bone scaffold is biodegradable, and the degradation rate is synchronized with the deposition rate of mineralized tissue. After 12 weeks of implantation, the weight loss rate of the bone scaffold is 10%-20%. At this time, the bone defect repair is in the bone plate formation and shaping stage, and the bone scaffold can provide sufficient mechanical support. After 12-24 months of implantation, the weight loss rate of the bone scaffold reaches 85%-95%, and the reduction in mechanical support caused by the degradation of the bone scaffold is compensated by the mechanical support provided by the newly formed tissue.

[0058] For example, in some embodiments of the present invention, the degradable shape memory material is polylactic acid (PLA), and in the mixed material, the mass ratio of the bone scaffold composite material to the degradable shape memory material is 1:2.

[0059] In some embodiments of the present invention, Figure 2 and Figure 3As shown, the bone scaffold is composed of a bone scaffold unit, each comprising a CaF2 unit cell structure framework 1 and a quadratic curve structure. The quadratic curve structure includes a quadratic curve body 2 located on each bottom surface of the CaF2 unit cell structure framework. The CaF2 unit cell structure framework 1 comprises a cubic framework composed of hollow straight rods, each of the eight vertices of the cubic framework having a short hollow rod. The quadratic curve body 2 comprises hollow curved rods, each having an inner diameter of 300-600 μm and an aperture of 300-600 μm. By varying the apertures of the hollow straight rods and hollow curved rods in the bone scaffold unit, the porosity of the bone scaffold can be controlled. The performance of the bone scaffold can also be modified by adjusting the shape of the quadratic curve body.

[0060] It should be noted that the quadratic curve body in the present invention is a structure composed of a hollow curved rod and having a shape similar to a quadratic curve. The quadratic curve includes one of an Archimedean spiral, a hyperbola, a parabola, a first function curve, a second function curve, and a third function curve; the first function curve satisfies the following functional relationship:

[0061] y=(0.09(5sin0.5x-2.5sinx) 2 -1) 4 ;

[0062] The second function curve satisfies the following functional relationship:

[0063] y=(0.09(5sin0.5x-2.5sinx) 2 -1.05) 4 ;

[0064] The third function curve satisfies the following functional relationship:

[0065] y=(0.08(5sin0.5x-2.5sinx) 2 -1) 4 ;

[0066] Among them, x is the horizontal coordinate and y is the vertical coordinate.

[0067] By adjusting the value of the independent variable x in the above function curve, the purpose of regulating the elastic modulus of the bone scaffold can be achieved. The adjustable range of the elastic modulus of the bone scaffold is 0.01-4.5GPa, which can match the mechanical strength of human cancellous bone (0.02-3.70GPa). It should be noted that the shape of the structure similar to a quadratic curve should be understood as the shape of the quadratic curve body being the same as the quadratic curve shape. For example, the shape of the structure similar to an Archimedean spiral should be understood as the shape of the quadratic curve body being the same as the Archimedean spiral.

[0068] Figure 4 and Figure 5 These are schematic diagrams of the structures of two types of quadratic curves. Figure 4 The secondary curve body is composed of four arc-shaped hollow tubes distributed in a spiral. Figure 5 The shape of the conic body is the same as the Archimedean spiral.

[0069] The present invention will be further described below with reference to specific examples. It should be understood that these examples are only intended to illustrate the present invention and are not intended to limit the scope of the present invention.

[0070] Example 1

[0071] 1.1. Dissolve CaCl2 in NH4Cl solution to obtain a first solution; dissolve NH4HCO3 and H2O4 in NH4Cl solution to obtain a second solution; in the first solution, the concentration of CaCl2 is 1 mol / L; in the second solution, the concentration of NH4HCO3 is 2 mol / L, and the concentration of H2O4 is 0.02 mol / L.

[0072] 1.2. Mix the first solution and the second solution, stir to react, filter, and dry to obtain the porous calcium carbonate.

[0073] 1.3. Mix porous calcium carbonate and eggshell powder in a mass ratio of 1:1 to obtain a first mixed powder; ball-mill calcium hydroxyphosphate and calcium titanate in a mass ratio of 1:1 to obtain a second mixed powder; mix the first mixed powder and the second mixed powder in a mass ratio of 2:1 to obtain a third mixed powder.

[0074] 1.4. Add the third mixed powder to water to obtain a first mixed solution; mix the PBS solution containing a bone tissue repair-promoting drug with the first mixed solution to obtain a second mixed solution; wherein the bone tissue repair-promoting drug is dexamethasone (Dex); in the second mixed solution, the concentration of the bone tissue repair-promoting drug is 1 g / L, and the concentration of the third mixed powder is 2.5 g / L.

[0075] 1.5. After the second mixed solution is subjected to a first stirring treatment, centrifugation is performed, and the precipitate is collected, washed, and dried to obtain nanoparticles loaded with the bone tissue repair-promoting drug; the first stirring treatment is performed for 12 hours.

[0076] 1.6. Add the nanoparticles loaded with the bone tissue repair drug to the dopamine solution to obtain a third mixed solution; after the third mixed solution is stirred for a second time, centrifugation is performed, the precipitate is collected, washed and dried to obtain a PDA-modified powder loaded with the bone tissue repair drug; in the third mixed solution, the concentration of the nanoparticles loaded with the bone tissue repair drug is 2.5 g / L, the concentration of the dopamine is 2.5 g / L, the second stirring treatment time is 12 h, and the dopamine solution is prepared by dissolving dopamine in Tris-HCl buffer.

[0077] 1.7. Add the PDA-modified powder loaded with a bone tissue repair drug to a silver nitrate solution, stir the reaction, centrifuge, collect the precipitate, wash, and dry to obtain a bone scaffold composite material; wherein the concentration of the silver nitrate solution is 1 mol / L, and the bone scaffold composite material has a layered structure, wherein the inner layer is a porous material loaded with a bone tissue repair drug, the middle layer is a polydopamine layer, and the outer layer is in situ grown Ag particles.

[0078] In addition, it should be noted that, although the present invention is disclosed as above, the scope of protection of the present invention is not limited thereto. Those skilled in the art may make various changes and modifications without departing from the spirit and scope of the present invention, and these changes and modifications will fall within the scope of protection of the present invention.

Claims

1. A method for preparing a bone scaffold composite material, characterized in that: include: Step S1, mixing porous calcium carbonate and eggshell powder in a mass ratio of 1: (0.9-1.1) to obtain a first mixed powder; ball-milling calcium hydroxyphosphate and calcium titanate in a mass ratio of 1: (0.9-1.1) to obtain a second mixed powder; mixing the first mixed powder and the second mixed powder in a mass ratio of (1.9-2.1): 1 to obtain a third mixed powder; Step S2, adding the third mixed powder to water to obtain a first mixed solution; mixing the PBS solution containing the bone tissue repair promoting drug with the first mixed solution to obtain a second mixed solution; Step S3, stirring the second mixed solution for the first time, centrifuging, collecting the precipitate, washing and drying to obtain nanoparticles loaded with the bone tissue repair promoting drug; Step S4, adding the nanoparticles loaded with the bone tissue repair promoting drug to the dopamine solution to obtain a third mixed solution; stirring the third mixed solution for a second time, centrifuging, collecting the precipitate, washing and drying to obtain a PDA-modified powder loaded with the bone tissue repair promoting drug; Step S5: adding the PDA-modified powder loaded with the bone tissue repair promoting drug into a silver nitrate solution, stirring for reaction, centrifuging, collecting the precipitate, washing and drying to obtain a bone scaffold composite material.

2. The method for preparing the bone scaffold composite material according to claim 1, wherein: In step S1, the method for preparing porous calcium carbonate includes: Step S11, dissolving CaCl2 in NH4Cl solution to obtain a first solution; dissolving NH4HCO3 and H2O4 in NH4Cl solution to obtain a second solution; Step S12: mixing the first solution and the second solution, stirring to react, filtering, and drying to obtain the porous calcium carbonate.

3. The method for preparing the bone scaffold composite material according to claim 2, characterized in that: In the first solution, the concentration of CaCl2 is 0.9-1.1 mol / L; in the second solution, the concentration of NH4HCO3 is 1.9-2.1 mol / L, and the concentration of H2O4 is 0.015-0.025 mol / L.

4. The method for preparing the bone scaffold composite material according to claim 1, characterized in that: In the second mixed solution, the concentration of the bone tissue repair promoting drug is 0.9-1.1 g / L, and the concentration of the third mixed powder is 2-3 g / L.

5. The method for preparing the bone scaffold composite material according to claim 1, characterized in that: In the third mixed solution, the concentration of the nanoparticles loaded with the bone tissue repair promoting drug is 2-3 g / L, and the concentration of dopamine is 2-3 g / L.

6. The method for preparing the bone scaffold composite material according to claim 1, characterized in that: The bone tissue repair promoting drug comprises one of dexamethasone, Kartogenin, cucurbitacin B and bone morphogenetic protein.

7. A bone scaffold composite material, characterized in that: The bone scaffold composite material is prepared by the preparation method according to any one of claims 1 to 6.

8. A bone scaffold, characterized in that: The bone scaffold is made of a mixed material through 4D printing, and the mixed material is made by mixing the bone scaffold composite material according to claim 7 and a degradable shape memory material.

9. The bone scaffold according to claim 8, characterized in that: The bone scaffold is composed of a bone scaffold unit, wherein the bone scaffold unit includes a CaF2-simulated unit cell structure frame (1) and a quadratic curve structure, wherein the quadratic curve structure includes a quadratic curve body (2) located on each bottom surface of the CaF2-simulated unit cell structure frame (1), wherein the CaF2-simulated unit cell structure frame (1) includes a cubic frame composed of hollow straight rods, wherein a hollow short rod is provided on each of the eight vertices of the cubic frame, and wherein the quadratic curve body (2) is composed of hollow curved rods, wherein the inner diameter of the hollow straight rod is 300-600 μm, and the aperture size of the hollow curved rod is 300-600 μm.

10. The bone scaffold according to claim 9, characterized in that: The quadratic curve body is a structure composed of hollow curved rods and having a shape similar to a quadratic curve. The quadratic curve includes one of an Archimedean spiral, a hyperbola, a parabola, a first function curve, a second function curve, and a third function curve. The first function curve satisfies the following functional relationship: y=(0.09(5sin0.5x-2.5sinx) 2 -1) 4 ; The second function curve satisfies the following functional relationship: y=(0.09(5sin0.5x-2.5sinx) 2 (1.05) 4 ; The third function curve satisfies the following functional relationship: y=(0.08(5sin0.5x-2.5sinx) 2 -1) 4 ; Among them, x is the horizontal coordinate and y is the vertical coordinate.

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