A temperature control device for cell culture

Abstract

The present invention discloses a temperature control device for cell culture, which relates to the field of cell culture technology, including: a base, a culture box, an operating component, a temperature control component and a culture component, wherein when the working end of the operating component is limited in the limit end of the culture component, the culture component rotates; when the working end of the operating component is limited in the limit end of the temperature control component, the working end of the temperature control component rises and falls. A temperature control device for cell culture according to an embodiment of the present invention performs zone control on the temperature control component and the culture component by setting an operating component, so that on the one hand, the heating temperature of the culture dish can be uniform, thereby effectively reducing the deviation during detection, and on the other hand, the storage shell that is farther from the opening can be gradually rotated to a position closer to the opening, so as to facilitate the storage and retrieval of the culture dish, and has great practicality.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture, and in particular to a temperature control device for cell culture. Background Art

[0002] Cell culture technology refers to the simulation of the in vivo environment in vitro, including sterility, suitable temperature, pH and certain nutritional conditions, so that cells can survive, grow, reproduce and maintain their main structure and function in the in vitro environment. Cell culture is also called cell cloning technology. In biology, the formal term is cell culture technology. During the cell culture process, the temperature of the cell culture environment needs to be controlled.

[0003] When storing and accessing culture dishes in existing cell culture cabinets, the access slots closer to the cabinet door are convenient for access, but the internal access slots farther from the cabinet door are not convenient for relevant staff to access. In addition, when controlling the temperature of cells in existing culture cabinets, the heating temperature of culture dishes close to or far from the heating source may be different, which may easily lead to deviations when testing cells.

[0004] Therefore, it is necessary to improve the existing temperature control device for cell culture to solve the above-mentioned related problems as much as possible. Summary of the invention

[0005] The present invention aims to solve one of the technical problems in the above-mentioned technology at least to some extent.

[0006] To achieve the above-mentioned purpose, the first aspect of the present invention proposes a temperature control device for cell culture, comprising: a base, an incubator, an operating component, a temperature control component and a culture component, wherein the incubator is arranged on the base, and a partition is arranged in the incubator to divide the incubator into an operating chamber and a working chamber, and a storage groove is opened on the inner bottom side of the incubator and is located in the working chamber; the operating component and the temperature control component are respectively arranged in the operating chamber, and the working end of the operating component and the working end of the temperature control component are respectively located in the working chamber; the culture component is rotatably arranged in the storage groove and is located directly below the working end of the operating component and the working end of the temperature control component; wherein, when the working end of the operating component is limited in the limit end of the culture component, the culture component rotates; when the working end of the operating component is limited in the limit end of the temperature control component, the working end of the temperature control component rises and falls.

[0007] In addition, the temperature control device for cell culture proposed in the present invention may also have the following additional technical features:

[0008] Furthermore, the operating component includes: a driving motor, a guide rod, an adjusting device, a first driving cylinder and a limiting ring, wherein the driving motor is arranged on the inner top side of the incubator through a fixing frame, and is located in the operating chamber, and the driving end of the driving motor extends downward; the guide rod is arranged on the driving end of the driving motor, and a first limiting groove is provided at the bottom of the guide rod; the adjusting device is lifted and lowered in the first limiting groove; the first driving cylinder is arranged on the partition through a fixing frame; the limiting ring is arranged on the driving end of the first driving cylinder, and the adjusting end of the adjusting device is limited at the bottom of the limiting ring.

[0009] Furthermore, a first through hole is formed on the partition plate and is coaxial with the guide rod, and the working end of the adjusting device passes through and extends from the first through hole.

[0010] Further, the adjusting device includes: a working rod, a connecting plate, a spring and an adjusting plate, wherein the working rod is lifted and lowered in the first through hole, and a first limit block is provided at the bottom of the working rod; the connecting plate is provided at the top of the working rod, and a second limit block is provided at the top of the connecting plate and is located in the first limit groove; the spring is sleeved on the working rod, and the two ends of the spring are respectively connected to the connecting plate and the partition; one end of the adjusting plate is provided on the connecting plate, and the other end is limited at the bottom of the limit ring.

[0011] Furthermore, the temperature control component includes: a lead screw, a slider, a controller, a heating element, a guide shell and a temperature sensor, wherein the lead screw is axially connected to the bottom of the partition and is coaxial with the first through hole, and a second through hole is provided inside the lead screw to penetrate to both ends, a second limit groove is provided at the bottom end of the lead screw, the bottom end of the working rod extends into the second through hole, and the first limit block is located in the second limit groove; the slider is arranged on the lead screw; the controller is arranged in the operating cavity; the heating element is arranged on the outer wall of the slider through a mounting frame; the guide shell is sleeved on the heating element, and the heating end of the heating element faces downward; the temperature sensor is arranged in the guide shell, and is electrically connected to the heating element and the controller respectively.

[0012] Furthermore, the culture component includes: a support rod, a connecting disk, a storage shell and a limit seat, wherein the support rod is axially connected in the storage groove; the connecting disk is arranged on the top of the support rod; multiple storage shells are respectively arranged on the connecting disk; the limit seat is arranged on the connecting disk and is coaxial with the working rod, and a third limit slot is opened on the top of the limit seat, and the first limit block cooperates with the third limit slot.

[0013] Furthermore, an opening is provided on one side of the incubator and is located at the working chamber, and a fourth limiting groove is provided on the inner wall of the opening, and a sealing structure is provided on the incubator.

[0014] Further, the sealing structure includes: a second driving cylinder, a cabin door and a sealing gasket, wherein the second driving cylinder is arranged on one side of the incubator through a fixing frame; the cabin door is arranged on the driving end of the second driving cylinder; the sealing gasket is arranged on one side of the cabin door and is located in the opening and the fourth limiting groove.

[0015] Furthermore, a humidity control device is provided in the operating chamber, and a humidity control end of the humidity control device extends into the working chamber.

[0016] According to a temperature control device for cell culture of the present invention, an operating component is set to control the temperature control component and the culture component in a zoned manner, so that on the one hand, the temperature of the culture dish can be evenly heated, thereby effectively reducing the deviation during detection; on the other hand, the storage shell that is farther away from the opening can be gradually rotated to a position closer to the opening, thereby facilitating the storage and retrieval of the culture dish, and the practicability is high. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the following description of the embodiments in conjunction with the accompanying drawings, in which:

[0018] Figure 1 is a schematic diagram of the three-dimensional structure of a temperature control device for cell culture according to one embodiment of the present invention;

[0019] Figure 2 is a schematic diagram of a three-dimensional structure of a sealing structure and a culture box of a temperature control device for cell culture according to an embodiment of the present invention;

[0020] Figure 3 is a schematic cross-sectional view of a three-dimensional structure of an incubator of a temperature control device for cell culture according to an embodiment of the present invention;

[0021] Figure 4 is a schematic diagram of the internal three-dimensional structure of a temperature control device for cell culture according to one embodiment of the present invention;

[0022] Figure 5 It is a schematic diagram of the three-dimensional structure decomposition of an operating component, a temperature control component and a culture component of a temperature control device for cell culture according to one embodiment of the present invention;

[0023] Figure 6 A temperature control device for cell culture according to an embodiment of the present invention Figure 5 A schematic diagram of the enlarged structure of the local A in the middle;

[0024] Figure 7 is a schematic diagram of the exploded three-dimensional structure of an operating component, a temperature control component and a culture component of another temperature control device for cell culture according to one embodiment of the present invention;

[0025] Figure 8 A temperature control device for cell culture according to an embodiment of the present invention Figure 7 A schematic diagram of the enlarged structure of the local B in the middle;

[0026] Fig. 9 A temperature control device for cell culture according to an embodiment of the present invention Figure 7 Schematic diagram of the enlarged structure of the local C in the middle;

[0027] As shown in the figure:

[0028] 1. Base; 2. Incubator; 21. Partition; 211. First through hole; 22. Operation chamber; 221. Humidity control device; 23. Working chamber; 24. Storage slot; 25. Opening; 251. Fourth limit slot; 26. Sealing structure; 261. Second drive cylinder; 262. Hatch door; 263. Sealing pad; 3. Operation assembly; 31. Drive motor; 32. Guide rod; 321. First limit slot; 33. Adjustment device; 331. Working rod; 3311. First limit block; 332, connecting plate; 3321, second limiting block; 333, spring; 334, adjusting plate; 34, first driving cylinder; 35, limiting ring; 4, temperature control component; 41, lead screw; 411, second through hole; 412, second limiting groove; 42, slider; 43, controller; 44, heating element; 45, guide shell; 46, temperature sensor; 5, culture component; 51, support rod; 52, connecting plate; 53, storage shell; 54, limiting seat; 541, third limiting groove. DETAILED DESCRIPTION

[0029] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and intended to be used to explain the present invention, but should not be understood as limiting the present invention.

[0030] A temperature control device for cell culture according to an embodiment of the present invention will be described below with reference to the accompanying drawings.

[0031] like Figures 1 to 4 As shown, a temperature control device for cell culture according to an embodiment of the present invention may include a base 1, a culture box 2, an operating component 3, a temperature control component 4 and a culture component 5.

[0032] Among them, the incubator 2 is arranged on the base 1, and a partition 21 is arranged in the incubator 2, and the incubator 2 is divided into an operation chamber 22 and a working chamber 23. A storage groove 24 is opened on the inner bottom side of the incubator 2 and is located in the working chamber 23. The operating component 3 and the temperature control component 4 are respectively arranged in the operating chamber 22, and the working end of the operating component 3 and the working end of the temperature control component 4 are respectively located in the working chamber 23. The culture component 5 is rotatably arranged in the storage groove 24 and is located directly below the working end of the operating component 3 and the working end of the temperature control component 4. When the working end of the operating component 3 is limited in the limit end of the culture component 5, the culture component 5 rotates; when the working end of the operating component 3 is limited in the limit end of the temperature control component 4, the working end of the temperature control component 4 rises and falls.

[0033] As a possible situation, according to another embodiment of the present invention, visual sensors (not marked in the figure) are respectively provided in the operating chamber 22 and the working chamber 23, and a camera screen (not marked in the figure) is provided on the incubator 2. The camera screen is electrically connected to the visual sensor, so that relevant staff can effectively monitor and inspect the interior through the camera screen.

[0034] like Figure 3 As shown, it should be noted that a first through hole 211 is opened on the partition 21 and is coaxial with the guide rod 32, and the working end of the adjustment device 33 passes through the first through hole 211 and extends, so as to limit the working rod 331 and avoid tilting or shaking.

[0035] like Figure 4 As shown, it should be noted that a humidity control device 221 is provided in the operating chamber 22, and the humidity control end of the humidity control device 221 extends into the working chamber 23, so that relevant staff can effectively adjust the air humidity in the working chamber 23 to maintain an ideal cell culture environment.

[0036] like Figure 4 As shown, it should be noted that the above-mentioned humidity control device 221 includes: a water tank, a humidifying disk, and an evaporation box.

[0037] like Figure 4 As shown, preferably, a carbon dioxide emission device and an ethylene oxide emission device are respectively provided in the operating chamber 22, and the emission interfaces of the carbon dioxide emission device and the ethylene oxide emission device are respectively located in the working chamber 23. On the one hand, by providing the carbon dioxide emission device, the carbon dioxide concentration required for cell culture is effectively maintained. On the other hand, by providing the ethylene oxide emission device, the interior of the working chamber 23 is effectively sterilized and disinfected.

[0038] As a possible situation, according to another embodiment of the present invention, an electrically controlled fan (not shown in the figure) is provided in the working chamber 23 to effectively ensure positive pressure in the working chamber 23 and uniform distribution and degradation efficiency of ethylene oxide.

[0039] Preferably, a UV lamp (not shown in the figure) is provided in the working chamber 23 to effectively perform preliminary surface disinfection therein.

[0040] As a possible situation, according to another embodiment of the present invention, a backup power supply (not shown in the figure) is provided on the incubator 2, which can effectively ensure the continuous normal operation of the equipment and avoid data loss on the one hand, and effectively improve the working efficiency and stabilize the voltage on the other hand, thereby preventing data damage.

[0041] like Figure 1 As shown, preferably, a master control platform is provided on the incubator 2, and the master control platform is electrically connected to the humidity control device 221, the second driving cylinder 261, the driving motor 31, the first driving cylinder 34, the controller 43, the carbon dioxide emission device, the ethylene oxide emission device, the electric-controlled fan, the UV lamp and the backup power supply, so that relevant staff can operate the relevant processes through the master control platform.

[0042] like Figure 2 and Figure 3 As shown, it should be noted that an opening 25 is provided on one side of the incubator 2 and is located at the working chamber 23, and a fourth limiting groove 251 is provided on the inner wall of the opening 25. A sealing structure 26 is provided on the incubator 2, which, to a certain extent, makes the working chamber 23 for storing cells relatively in a sealed state.

[0043] like Figure 1 and Figure 2 As shown, in one embodiment of the present invention, the sealing structure 26 includes: a second driving cylinder 261 , a hatch 262 and a sealing gasket 263 .

[0044] Among them, the second driving cylinder 261 is set on one side of the incubator 2 through a fixing frame, the hatch 262 is set on the driving end of the second driving cylinder 261, and the sealing gasket 263 is set on one side of the hatch 262 and is located in the opening 25 and the fourth limiting groove 251.

[0045] It should be noted that the sealing gasket 263 is made of rubber, so that the sealing gasket 263 itself can be expanded and contracted by external force, so that the hatch 262 fits the opening 25 more closely during the locking process.

[0046] like Figure 5 , Figure 7 and Figure 8As shown, in one embodiment of the present invention, the operating assembly 3 includes: a driving motor 31 , a guide rod 32 , an adjusting device 33 , a first driving cylinder 34 and a limiting ring 35 .

[0047] Among them, the driving motor 31 is arranged on the inner top side of the incubator 2 through a fixing frame and is located in the operating chamber 22, and the driving end of the driving motor 31 extends downward, the guide rod 32 is arranged on the driving end of the driving motor 31, and a first limiting groove 321 is opened at the bottom of the guide rod 32, the adjusting device 33 is lifted and lowered in the first limiting groove 321, the first driving cylinder 34 is arranged on the partition 21 through a fixing frame, the limiting ring 35 is arranged on the driving end of the first driving cylinder 34, and the adjusting end of the adjusting device 33 is limited at the bottom of the limiting ring 35.

[0048] like Figure 5 and Figure 6 As shown, to clearly illustrate the previous embodiment, in one embodiment of the present invention, the adjusting device 33 includes: a working rod 331 , a connecting plate 332 , a spring 333 and an adjusting plate 334 .

[0049] Among them, the working rod 331 is lifted and lowered in the first through hole 211, and a first limit block 3311 is arranged at the bottom of the working rod 331, the connecting plate 332 is arranged at the top of the working rod 331, and a second limit block 3321 is arranged on the top of the connecting plate 332 and is located in the first limit groove 321, the spring 333 is sleeved on the working rod 331, and the two ends of the spring 333 are respectively connected to the connecting plate 332 and the partition 21, one end of the adjusting plate 334 is arranged on the connecting plate 332, and the other end is limited at the bottom of the limit ring 35.

[0050] like Figure 5 , Figure 7 and Fig. 9 As shown, in one embodiment of the present invention, the temperature control assembly 4 includes: a lead screw 41 , a slider 42 , a controller 43 , a heating element 44 , a guide shell 45 and a temperature sensor 46 .

[0051] Among them, the lead screw 41 is axially connected to the bottom of the partition 21 and is coaxial with the first through hole 211, and a second through hole 411 is opened inside the lead screw 41 to penetrate to both ends, and a second limiting groove 412 is opened at the bottom end of the lead screw 41, and the bottom end of the working rod 331 extends into the second through hole 411, and the first limiting block 3311 is located in the second limiting groove 412, the slider 42 is set on the lead screw 41, and the controller 43 is set in the operating chamber 22, the heating element 44 is set on the outer wall of the slider 42 through the mounting frame, the guide shell 45 is sleeved on the heating element 44, and the heating end of the heating element 44 faces downward, and the temperature sensor 46 is set in the guide shell 45, and is electrically connected with the heating element 44 and the controller 43 respectively.

[0052] like Figure 5 and Figure 7 As shown, in one embodiment of the present invention, the culture component 5 includes: a support rod 51, a connecting plate 52, a storage shell 53 and a limiting seat 54.

[0053] Among them, the support rod 51 is axially connected to the storage groove 24, the connecting plate 52 is arranged on the top of the support rod 51, and multiple storage shells 53 are respectively arranged on the connecting plate 52. The limit seat 54 is arranged on the connecting plate 52 and is coaxial with the working rod 331, and a third limit groove 541 is opened on the top of the limit seat 54, and the first limit block 3311 cooperates with the third limit groove 541.

[0054] As a possible situation, according to another embodiment of the present invention, the first limiting groove 321, the second limiting groove 412, and the third limiting groove 541 can also be cross-shaped grooves, and the first limiting block 3311 and the second limiting block 3321 can also be cross-shaped blocks to facilitate mutual limiting thereof.

[0055] It should be noted that the size of the connecting plate 52 is equal to the size of the guide shell 45, which effectively allows multiple cell culture dishes to be within the heating source.

[0056] Specifically, when the relevant staff needs to use a temperature control device for cell culture according to an embodiment of the present invention, they first turn on the second driving cylinder 261 to open the hatch 262 , and place the cell culture dishes containing cells into the multiple storage shells 53 .

[0057] When the worker cannot place the cell culture dish in the storage shell 53 which is far away from the opening 25 , the first driving cylinder 34 is turned on through the master control platform.

[0058] The first driving cylinder 34 works, and the working end of the first driving cylinder 34 drives the limiting ring 35 to descend, thereby pressing the adjusting plate 334, and then driving the connecting plate 332 to descend, thereby driving the working rod 331 to descend along the first through hole 211 and the second through hole 411 in sequence, and then driving the first limiting block 3311 to gradually disengage from the second limiting groove 412 and insert into the third limiting groove 541. At the same time, the connecting plate 332 drives the second limiting block 3321 to descend, and the spring 333 is compressed.

[0059] It should be noted that when the first limiting block 3311 is inserted into the third limiting groove 541 and the second limiting block 3321 is in the first limiting groove 321 , the first driving cylinder 34 is turned off and the driving motor 31 is turned on.

[0060] The driving motor 31 works, and the driving end of the driving motor 31 drives the guide rod 32 to rotate, which in turn drives the second limit block 3321, the connecting plate 332, the adjusting plate 334, the working rod 331 and the first limit block 3311 to rotate, and then drives the limit seat 54 to rotate, thereby driving the connecting disk 52 and the cell culture dishes in the multiple storage shells 53 to rotate in turn.

[0061] When the farther storage shell 53 rotates to be closer to the exit 25, the relevant staff turns off the driving motor 31 and places the cell culture dish therein.

[0062] When the relevant staff needs to heat the cells evenly, they turn on the first driving cylinder 34 through the main control platform to drive the limit ring 35 to rise. At this time, due to the force of the spring 333 itself, the connecting plate 332, the adjustment plate 334, the working rod 331 and the first limit block 3311 are driven to rise in turn, so that the first limit block 3311 gradually disengages from the third limit groove 541 and is inserted into the second limit groove 412. When the rising work is completed, the relevant staff closes the first driving cylinder 34 and starts the drive motor 31.

[0063] The drive motor 31 works, and the driving end of the drive motor 31 drives the first limit block 3311 to rotate, thereby driving the lead screw 41 to rotate, thereby driving the slider 42 to descend, and then driving the heating element 44, the guide shell 45 and the temperature sensor 46 to descend in turn, so that the heating source gradually approaches the cell culture dish. At this time, the drive motor 31 is turned off and the controller 43 is turned on through the main control platform, so that the heating element 44 works and the temperature of the cells can be controlled.

[0064] In summary, according to a temperature control device for cell culture according to an embodiment of the present invention, an operating component 3 is provided to perform zone control on the temperature control component 4 and the culture component 5, so that on the one hand, the heating temperature of the culture dish can be uniform, thereby effectively reducing the deviation during detection; on the other hand, the storage shell 53 that is farther away from the opening 25 can be gradually rotated to a position closer to the opening 25, thereby facilitating the storage and retrieval of the culture dish, and the practicability is high.

[0065] In the description of this specification, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of this application, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0066] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0067] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations on the present application. Ordinary technicians in this field can change, modify, replace and modify the above embodiments within the scope of the present application.

Claims

1. A temperature control device for cell culture, characterized in that: include: A base (1), an incubator (2), an operating component (3), a temperature control component (4) and a culture component (5), wherein: The incubator (2) is arranged on the base (1), and a partition (21) is arranged inside the incubator (2) to divide the incubator (2) into an operation chamber (22) and a working chamber (23); a storage groove (24) is provided on the bottom side of the interior of the incubator (2) and is located inside the working chamber (23); The operating component (3) and the temperature control component (4) are respectively arranged in the operating chamber (22), and the working end of the operating component (3) and the working end of the temperature control component (4) are respectively located in the working chamber (23); The culture component (5) is rotatably disposed in the storage tank (24) and is located directly below the working end of the operating component (3) and the working end of the temperature control component (4); Wherein, when the working end of the operating component (3) is limited within the limiting end of the culture component (5), the culture component (5) rotates; when the working end of the operating component (3) is limited within the limiting end of the temperature control component (4), the working end of the temperature control component (4) rises and falls; The operating assembly (3) comprises: a driving motor (31), a guide rod (32), an adjusting device (33), a first driving cylinder (34) and a limiting ring (35), wherein: The drive motor (31) is arranged on the inner top side of the incubator (2) via a fixing frame and is located in the operating chamber (22), and the drive end of the drive motor (31) extends downward; The guide rod (32) is arranged on the driving end of the driving motor (31), and a first limiting groove (321) is provided at the bottom of the guide rod (32); The adjusting device (33) is arranged in the first limiting groove (321) for lifting; The first driving cylinder (34) is arranged on the partition (21) via a fixing frame; The limiting ring (35) is arranged on the driving end of the first driving cylinder (34), and the adjusting end of the adjusting device (33) is limited at the bottom of the limiting ring (35); The partition plate (21) is provided with a first through hole (211) which is coaxial with the guide rod (32), and the working end of the adjustment device (33) passes through the first through hole (211) and extends; The adjusting device (33) comprises: a working rod (331), a connecting plate (332), a spring (333) and an adjusting plate (334), wherein: The working rod (331) is lifted and lowered in the first through hole (211), and a first limiting block (3311) is provided at the bottom of the working rod (331); The connecting plate (332) is arranged on the top of the working rod (331), and a second limiting block (3321) is arranged on the top of the connecting plate (332) and is located in the first limiting groove (321); The spring (333) is sleeved on the working rod (331), and two ends of the spring (333) are respectively connected to the connecting plate (332) and the partition plate (21); One end of the adjustment plate (334) is arranged on the connection plate (332), and the other end is limited at the bottom of the limiting ring (35); The temperature control component (4) comprises: a lead screw (41), a slider (42), a controller (43), a heating element (44), a guide shell (45) and a temperature sensor (46), wherein: The lead screw (41) is axially connected to the bottom of the partition (21) and is coaxial with the first through hole (211), and a second through hole (411) is provided inside the lead screw (41) and penetrates to both ends, a second limiting groove (412) is provided at the bottom end of the lead screw (41), the bottom end of the working rod (331) extends into the second through hole (411), and the first limiting block (3311) is located in the second limiting groove (412); The sliding block (42) is arranged on the lead screw (41); The controller (43) is arranged in the operating chamber (22); The heating element (44) is arranged on the outer wall of the sliding block (42) via a mounting frame; The guide shell (45) is sleeved on the heating element (44), and the heating end of the heating element (44) faces downward; The temperature sensor (46) is disposed in the guide shell (45), and is electrically connected to the heating element (44) and the controller (43) respectively; The culture assembly (5) comprises: a support rod (51), a connection plate (52), a storage shell (53) and a limiting seat (54), wherein: The support rod (51) is axially connected and disposed in the storage groove (24); The connecting plate (52) is arranged on the top of the supporting rod (51); The plurality of storage shells (53) are respectively arranged on the connection plate (52); The limiting seat (54) is arranged on the connecting disk (52) and is coaxial with the working rod (331), and a third limiting groove (541) is provided on the top of the limiting seat (54), and the first limiting block (3311) cooperates with the third limiting groove (541).

2. A temperature control device for cell culture according to claim 1, characterized in that: An opening (25) is provided on one side of the incubator (2) and is located at the working chamber (23), and a fourth limiting groove (251) is provided on the inner wall of the opening (25). A sealing structure (26) is provided on the incubator (2).

3. A temperature control device for cell culture according to claim 2, characterized in that: The sealing structure (26) comprises: a second driving cylinder (261), a cabin door (262) and a sealing gasket (263), wherein: The second driving cylinder (261) is arranged on one side of the incubator (2) via a fixing frame; The cabin door (262) is arranged on the driving end of the second driving cylinder (261); The sealing gasket (263) is arranged on one side of the cabin door (262) and is located within the opening (25) and the fourth limiting groove (251).

4. A temperature control device for cell culture according to claim 1, characterized in that: A humidity control device (221) is provided in the operating chamber (22), and a humidity control end of the humidity control device (221) extends into the working chamber (23).

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