A method for breeding low-temperature resistant babylonia and a method for breeding babylonia at low temperature

Abstract

The present invention discloses a breeding method for low-temperature resistant babylonia. The breeding method, under the premise of using a low-temperature resistant adjuvant, obtains a first-generation low-temperature resistant basic population through three-stage domestication, and on this basis, the basic population is ripened and the offspring are cultivated to obtain a second-generation low-temperature resistant basic population, and the above basic population ripening and offspring cultivation are further repeated, and finally the babylonia with excellent low-temperature resistance is successfully cultivated. The low-temperature resistant babylonia still has an extremely high survival rate when cultivated in a low-temperature environment, and the specifications and yield of the babylonia are also relatively excellent.

Description

Technical Field

[0001] The present invention belongs to the technical field of snail cultivation, and more specifically, relates to a method for breeding low-temperature-resistant babylonia snails and a method for cultivating babylonia snails at low temperatures. Background Art

[0002] Dongfeng conch is commonly known as flower conch, prosperous conch, and yellow conch. In Taiwan, it is also known as phoenix conch. It belongs to the phylum Mollusca, class Gastropoda, subclass Prosobranchia, order Neogastropoda, family Buccinidae, and genus Babylonia. It is a carnivorous shallow-sea benthic shellfish with delicious taste. It is a kind of high-protein, low-fat precious seafood that meets modern nutritional needs.

[0003] Water temperature is one of the important factors that affect the reproduction, development, growth, metabolism, and geographical distribution of aquatic animals. At present, the Dongfeng snail is a tropical and subtropical shellfish. Its breeding area is mainly in the southeast coastal area and is distributed in tropical and subtropical areas. Dongfeng snail is not resistant to low temperatures, and its variable temperature characteristics make it very sensitive to changes in the surrounding temperature. The document "The Effect of Temperature and Salinity on Embryonic Development and Larval Growth of Square Spotted Dongfeng Snail" discloses that the suitable water temperature for the embryonic development of square spot Dongfeng snail is 23-31°C, and the optimal water temperature is 25-30°C. When the water temperature is lower than 20°C (14°C, 16°C, 18°C), the embryonic development of Dongfeng snail basically remains in the initial stage. When the water temperature is 20°C, the hatching rate of the egg sac is extremely low and the deformity rate is extremely high. In addition, temperature will greatly affect the growth rate of Dongfeng snail larvae. When the temperature is 23°C, the Dongfeng snail larvae grow slowly, and their growth rate is only 7.0μm·d -1 It can be seen that low temperature has an all-round impact on the snails. It not only affects the development of the snail embryos and the hatching of the egg sacs, but also affects the growth of the larvae. At present, it is not possible to directly breed snails in the winter in northern my country.

[0004] Therefore, the existing baby snails have many problems at low temperatures, and are difficult to adapt to low-temperature aquaculture, which seriously restricts the large-scale aquaculture development of baby snails. How to provide a breeding method for low-temperature resistant baby snails is crucial to stabilizing the baby snail aquaculture industry. Summary of the invention

[0005] In view of the above-mentioned existing technical problems, the primary purpose of the present invention is to provide a method for breeding low-temperature-resistant Babylonia snails. The Babylonia snails bred by the breeding method have excellent low-temperature resistance and still have a very high survival rate when cultured in a low-temperature environment. The specifications and yields of the Babylonia snails are also relatively excellent.

[0006] The second object of the present invention is to provide a method for cultivating Babylonia paniculata at low temperature.

[0007] In order to achieve the above object, the present invention is implemented by the following technical solutions:

[0008] The present invention claims protection for a method for breeding low-temperature resistant Babylonia snails, comprising the following steps:

[0009] S1. The first generation of low temperature tolerant basic population:

[0010] The first stage is cooling the larval stage: During the early, middle and late larval stages, the aquaculture water is cooled at least once to 16-18°C. Each cooling is maintained for 6-24 hours before being warmed up to normal temperature.

[0011] The second stage is cooling during the intermediate cultivation period: During the cultivation of juvenile Dongfeng snails, the temperature is lowered at least three times. The aquaculture water body is cooled to 16-18℃ for the first time, 14-16℃ for the second time, and 12-14℃ for the third and subsequent times. Each cooling is maintained for 12-24 hours before the temperature is raised to normal temperature.

[0012] The third stage is cooling during the grow-out period: During the grow-out period, the water temperature is cooled several times, starting from 16-18°C, and the water temperature gradually decreases. The last cooling is reduced to 8-10°C, and each cooling is maintained for 24-48 hours before the temperature is raised to normal temperature.

[0013] Feeding during the breeding period of the first generation of low-temperature tolerant basic population;

[0014] After the third stage of the growth period, when the temperature drops, the top 40-70% of the Babylonia snails are selected based on their weight to obtain the first generation of low-temperature tolerant basic population;

[0015] S2. Basic population mating: The first generation of low-temperature resistant basic population is selected for interbreeding and breeding until the gonads of the Babylonia snails mature, mating is performed, and egg sacs are collected;

[0016] S3. Progeny cultivation: After the oocysts in step S2 are disinfected, they are incubated and cultivated; during the cultivation period, the above step S1 is repeated to obtain a second generation breeding cold-resistant base population;

[0017] S4. After the second generation of low-temperature resistant basic population is bred, steps S2 and S3 are repeated, and the water temperature is maintained at 10 to 18°C ​​for breeding, so as to obtain low-temperature resistant Babylonia snails;

[0018] In the steps S1 to S4, a low-temperature resistant additive is added to the aquaculture water; the low-temperature resistant additive includes, by weight, 20 to 30 parts of trehalose, 20 to 30 parts of arachidonic acid, and 40 to 60 parts of proline.

[0019] Under the premise of using a low-temperature resistant adjuvant, the present invention obtains the first generation of low-temperature resistant basic population through three-stage domestication, and on this basis, the basic population is ripened and the offspring are cultivated to obtain the second generation of low-temperature resistant basic population, and the second generation of low-temperature resistant basic population is further repeated to ripen the basic population and cultivate the offspring, and finally successfully cultivates Babylonia snails with excellent low-temperature resistance. Specifically:

[0020] The present invention carries out the first stage of domestication in the planktonic larval stage of the baby snails, and respectively performs cooling in the early veliger larval stage, the middle veliger larval stage and the late veliger larval stage of the baby snails; then carries out the second stage of domestication in the juvenile baby snail cultivation stage, and gradually adapts the juvenile baby snails to the low temperature stress through multiple step-by-step cooling; finally, carries out the third stage of domestication in the adult baby snail cultivation stage, and further stimulates and domesticates the baby snails; through three domestications, the body weight is used as a screening index, and the best are screened and retained to obtain the first generation of low temperature resistant basic population for breeding.

[0021] Then the first generation of low-temperature resistant basic population is mixed and bred, the egg sacs are collected, and then the above-mentioned step-by-step cooling operation is repeated to obtain the second generation of breeding basic population. Then the second generation of breeding basic population continues to repeat steps S2 and S3, and finally the water temperature is maintained at 8-10°C for breeding, and the low-temperature resistant Babylonia snails can be obtained.

[0022] Through research, the inventors found that, on the one hand, under the above-mentioned specific low temperature stress and step-by-step cooling method, the content of various osmotic regulating substances in the body of the Dongfeng snail will change, thereby stimulating the Dongfeng snail to form a variety of osmotic regulating abilities, thereby improving the Dongfeng snail's resistance to low temperature; on the other hand, after multi-stage and multiple screening and fixation of low-temperature resistance traits, the Dongfeng snail has low-temperature immune memory.

[0023] Furthermore, the inventors found through research that under low temperature stress, the content of various osmotic regulating substances in the body of the baby snail will change, which will greatly affect the hatching, development, growth and survival of the baby snail. The inventors found through research that trehalose, arachidonic acid and proline are one of the important osmotic regulating substances in the body of the baby snail, and they are closely related to the cold resistance of the baby snail. During the breeding process, the inventors added low temperature resistant additives to the aquaculture water, thereby assisting in improving the adaptability of the baby snail to low temperatures.

[0024] The present invention uses the above-mentioned specific low-temperature breeding method and is supplemented with low-temperature resistant additives to finally breed low-temperature resistant Babylonia snails with excellent low-temperature resistance. The Babylonia snails still have a very high survival rate when cultivated in a low-temperature environment, and the specifications and yields of the Babylonia snails are also relatively excellent.

[0025] Preferably, in step S1, at least one of the following (a) to (c) is selected:

[0026] (a) When cooling the larvae in the first stage, the interval between each cooling is 2 to 6 days;

[0027] (b) During the second stage of the intermediate incubation period, the temperature is lowered every 6 to 12 days;

[0028] (c) When cooling during the third stage of growth, the interval between each cooling is 20 to 40 days

[0029] Preferably, in step S1, at least one of the following (d) to (f) is selected:

[0030] (d) When the temperature drops during the first stage of larval stage, the amount of low temperature resistant additive added is 0.5-3 ppm;

[0031] (e) During the second stage of the intermediate incubation period, when the temperature drops, the amount of low-temperature resistant additive added is 0.1 to 2 grams per kilogram of bait;

[0032] (f) When the temperature drops during the third stage of the culture period, the amount of cold-resistant additive added is 0.1 to 2 grams per kilogram of bait.

[0033] Preferably, when the temperature drops during the first stage larval stage, the cold-resistant additive is added once every two days; when the temperature drops during the second stage intermediate cultivation period, the bait is fed twice a day; when the temperature drops during the third stage rearing period, the bait is fed once every two days.

[0034] Preferably, the feeding amount of the bait accounts for 8-12% of the body weight of the Babylonia snail.

[0035] Preferably, in step S1, when cooling the water during the third stage of the cultivation period, lowering the water temperature twice constitutes one small cycle, and the water temperature is gradually lowered between each small cycle, which lasts for 4 to 6 small cycles.

[0036] More specifically, during the third stage of the cultivation period, the first and second cooling times can be 16-18°C; the third and fourth cooling times can be 14-16°C; the fifth and sixth cooling times can be 12-14°C; the seventh and eighth cooling times can be 10-12°C; and the ninth and tenth cooling times can be 8-10°C.

[0037] Preferably, in step S1, the bait is selected from one or more of clams, mussels, oysters, scallop meat, sardines, and blue round scad.

[0038] In step S3, when step S1 is repeated during the incubation period, at least one of the following (g) to (i) is selected:

[0039] (g) When cooling the larvae in the first stage, the interval between each cooling is 1 to 4 days;

[0040] (h) During the second stage of the intermediate incubation period, the temperature is lowered every 3 to 8 days;

[0041] (i) During the third stage of the growing period, the temperature is lowered with an interval of 5 to 20 days between each cooling.

[0042] In step S3, by shortening the acclimation intervals between the three stages (i.e., the cooling intervals), the baby snails can be further stimulated, and the baby snails can be better acclimated and adapted to low temperatures.

[0043] Preferably, in step S2, enhanced ripening is carried out during the breeding period to accelerate the development and maturation of the gonads of the Babylonia snails.

[0044] Preferably, the enhanced ripening process refers to: feeding crabs and oysters, or feeding oyster meat. More specifically, in step S2, feeding is continued for 3 to 4 days and then stopped for 1 day; the feeding amount of crabs and oysters accounts for 14 to 16% of the first-generation parent body weight of Dongfeng snails; or the feeding amount of oyster meat accounts for 8 to 12% of the first-generation parent body weight of Dongfeng snails.

[0045] Preferably, in step S3, unicellular algae are used for feeding and cultivating larvae. More specifically, unicellular algae conventionally used for cultivation of baby snails in the art can be used for feeding baby snails; unicellular algae such as shrimp pond algae, golden algae, flat algae or spirulina.

[0046] Preferably, in steps S1 to S4, the salinity is controlled at 28 to 30 ppt, the dissolved oxygen is ≥ 5 mg / L, and the pH is 7.9 to 8.1.

[0047] Preferably, in step S4, the baby snails are cultured until the size is above 50 heads / catties.

[0048] Preferably, in step S2, the ratio of the number of mating female Babylonia snails to the number of mating male Babylonia snails is 1:1-3.

[0049] Specifically, the specifications of the basic population of Dongfeng snails for breeding can be: shell height 4.0-6.05cm, 60-80 snails / kg, bright shell surface color, normal pattern, no damage to the shell, soft abdominal foot, not hardened front foot, normal vitality, soft and plump body.

[0050] Specifically, the babylonia is of the genus Babylonia (Babylonia), including but not limited to Babylonia areolata, Babylonia lutosa, Babylonia formosae, etc.

[0051] Specifically, in the present invention, normal temperature is the water temperature under natural conditions without human intervention; more specifically, normal temperature can be the conventional temperature for breeding of baby snails in this field; more specifically, normal temperature can be 20-31°C, and the normal temperature in the present invention can be 21°C, 23°C, 25°C, 27°C, 29°C, 31°C, etc., or an interval range formed by any of the above numerical values, and the present invention is not limited to this.

[0052] Furthermore, the present invention seeks to protect a method for breeding Babylonia sinensis under low temperatures, wherein a low-temperature resistant adjuvant is added during the breeding process of the low-temperature resistant Babylonia sinensis bred by the above breeding method.

[0053] Preferably, the amount of the low temperature resistant additive added is 0.01-0.02% of the total weight of the Babylonia snail. Further preferably, the amount of the low temperature resistant additive added is 0.015-0.018% of the total weight of the Babylonia snail.

[0054] Preferably, the temperature during the breeding process is 8 to 18° C. Further preferably, the temperature during the breeding process is 12 to 16° C. More preferably, the temperature during the breeding process is 14 to 16° C.

[0055] Preferably, feed is given at least once every two days.

[0056] Preferably, the stocking density of Babylonia snails is 800 to 1500 per m 2 .

[0057] Compared with the prior art, the present invention has the following beneficial effects:

[0058] The present invention uses the above-mentioned specific low-temperature breeding method and is supplemented with low-temperature resistant additives to finally breed low-temperature resistant Babylonia snails with excellent low-temperature resistance. The Babylonia snails still have a very high survival rate when cultivated in a low-temperature environment, and the specifications and yields of the Babylonia snails are also relatively excellent. DETAILED DESCRIPTION

[0059] The present invention is further described below with reference to specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0060] The phrase "early veliger larvae" refers to the developmental period in which two residual yolk masses are clearly visible in the body of the Babylonia snail larvae, and there are two oval veligers that are symmetrical on both sides and have densely ciliated edges.

[0061] The phrase "mid-stage veliger" refers to the developmental period after the disappearance of the main characteristic yolk mass in the body of the Babylonia snail larvae.

[0062] The phrase "late veliger larvae" refers to the developmental period when the veliger larvae open their veliger and swim like a butterfly, and develop features such as the proboscis, gill filaments, red pigment, and pigment spots.

[0063] The phrase "intermediate cultivation period" refers to the period from the metamorphosis of the planktonic larvae of the Babylonia snails to the cultivation of snail seedlings with a shell height of 0.5 to 1.5 cm.

[0064] The phrase "growth period" refers to the period from the time when snail seedlings with a shell height of 0.5 to 1.5 cm are cultivated to large snails of commercial size.

[0065] Example 1 A method for breeding low-temperature resistant Babylonia

[0066] (1) The first generation of low temperature tolerant basic population:

[0067] 1) Cooling of the larvae in the first stage: A plastic basket (Φ60m, 20cm high) was suspended in a cement pool (7.0m×5.0m×1.4m) as a hatching device for the egg capsules of Babylonia square. There are two dark sedimentation tanks outdoors with a total volume of 720m 3 After the seawater in the sea area is settled in the dark sedimentation tank for 24 hours, it flows into the sand filter tank. After two sand filtrations, it is pumped to the protein separator by a water pump and then enters the nursery room after ultraviolet disinfection. Incubation conditions: water temperature 28-30℃, salinity 28-29, pH 7.9-8.1, dissolved oxygen ≥5mg / L, continuous aeration, and the air stone in the nursery pool is maintained at 1 / m 2 ,, change the water once a day, the water change volume is 100%, observe the embryonic development every day, measure the water temperature, salinity, and pH value, and remove dead oocysts in time to avoid affecting the water quality.

[0068] When the baby snails hatch from the egg capsules, the density of the baby snails is controlled at 75,000 per cubic meter. During the culture of baby snails, the water is cooled at least once in the early, middle and late stages of the larvae to 17°C. Each cooling is maintained for 12 hours before being raised to normal temperature. Each cooling is separated by 5 days.

[0069] 2) Cooling during the second stage of the intermediate cultivation period: When the planktonic larvae of Dongfeng snails develop to the late veliger larvae, they are fed with benthic diatoms. When they metamorphose into crawling juvenile snails, they are collected and transferred to a pool covered with fine sand (2.0m×5.0m×0.8m). The stocking density is 15,000 per square meter. The top of the pool is shaded with a single layer of black sunshade nets, and Artemia larvae are fed. Feeding is done once in the morning and evening every day. The amount of feeding is based on the amount of residual bait. The experimental conditions are: water temperature 28-30℃, salinity 28-29, pH7.9-8.1, dissolved oxygen ≥5mg / L. Change the water 500%-800% every day.

[0070] During the cultivation of young snails, the water was cooled three times. The first cooling was to 17°C, the second cooling was to 15°C, and the third cooling was to 13°C. Each cooling was maintained for 12 hours before returning to normal temperature. Each cooling was separated by 8 days.

[0071] 3) Cooling during the third stage of the culture period: When the juvenile snails develop into adult snails, the temperature is lowered several times during the cultivation of adult snails, with 2 water temperature reductions as one small cycle, which lasts for 5 small cycles. The water temperature is gradually reduced between each small cycle. The water temperature is lowered to 9°C for the last time. The temperature is maintained for 36 hours each time and then raised to normal temperature. There is an interval of 30 days between each cooling. The specific cooling operations are: the first and second coolings are to 16-18°C; the third and fourth coolings are to 14-16°C; the fifth and sixth coolings are to 12-14°C; the seventh and eighth coolings are to 10-12°C; the ninth and tenth coolings are to 8-10°C.

[0072] Adult snails were cultured in pools with a length, width and height of 2.0m×5.0m×0.8m respectively. A 10cm high square plastic board was laid at the bottom of each cement pool, and a 60-mesh silk screen was hung on the plastic board. The thickness of the sand was 5cm. During the experiment, the water in the pool was sand filtered twice in advance, and the daily water change was more than 600%. The pool water was drained every half a month, and the sand was flushed with water to loosen and remove dirt. The light intensity was controlled between 500-1000lx. Before putting the snails, 100mg / L bleaching powder was mixed with water and sprinkled on the cement pool, and rinsed with fresh water after 24 hours. Sand layer disinfection: After the sand was laid and rinsed, water was added to make it 5cm higher than the sand layer. It was soaked in 100mg / L formaldehyde solution for 12 hours and then drained. It was rinsed with fresh water 3 times until it was clean. The water depth was 40cm.

[0073] During the breeding period, the first generation of selected and bred low-temperature resistant basic groups were fed with scallop meat and blue round scads as bait. The amount of bait fed accounted for 8-12% of the weight of the adult snails. The surviving individuals were selected, and the top 50% by weight with intact appearance and no individual damage were retained to obtain the first generation of selected and bred low-temperature resistant basic groups.

[0074] (2) Basic population mating promotion: salinity is controlled at 28-30, water temperature at 28-29℃, dissolved oxygen ≥5mg / L, pH 7.9-8.1, and the water level in the pond is maintained at 30-40cm. The first generation of low-temperature resistant basic population is selected for mixed mating (the ratio of female snails to male snails is 1:2), and during the breeding period, intensive mating promotion is carried out (feeding crabs and oysters, feeding for 3-4 consecutive days and stopping for 1 day; the feeding amount of crabs and oysters accounts for 15% of the body weight of the snails) until the snails' gonads mature. When the snails mature, they mate naturally. Female snails usually lay eggs at night and collect egg sacs.

[0075] (3) Offspring cultivation: the egg cysts obtained in step (2) are disinfected, hatched, and fed with shrimp pond algae to cultivate the planktonic larvae of Babylonia sinensis; the above step (1) is repeated during the cultivation period, wherein the interval between each cooling during the larval stage in the first stage is changed to 4 days, the interval between each cooling during the intermediate cultivation stage in the second stage is changed to 6 days, and the interval between each cooling during the growth stage in the third stage is changed to 15 days, thereby obtaining the second generation breeding basic population.

[0076] (4) Repeat steps (2) and (3) for the second generation breeding base population, and maintain the water temperature at 10-18°C for breeding to obtain low-temperature resistant Babylonia snails.

[0077] During the cultivation period, a low-temperature resistant additive is used in the aquaculture water body. The low-temperature resistant additive includes, by weight, 25 parts of trehalose, 25 parts of arachidonic acid, and 50 parts of proline.

[0078] Among them, when the temperature drops during the first stage larval stage, the amount of cold-resistant additives added is 2ppm (based on the breeding pond water), and it is added once every two days; when the temperature drops during the second stage intermediate cultivation period, the amount of cold-resistant additives added is 1.5 grams per kilogram of bait, and the bait is fed twice a day; when the temperature drops during the third stage growing period, the amount of cold-resistant additives added is 1.5 grams per kilogram of bait, and the bait is fed once every two days.

[0079] Example 2 A method for breeding low-temperature resistant Babylonia

[0080] The difference between this embodiment and embodiment 1 is that, in terms of mass percentage, the low-temperature resistant auxiliary agent includes: 20 parts of trehalose, 30 parts of arachidonic acid, and 40 parts of proline.

[0081] Example 3 A breeding method for low temperature resistant Babylonia

[0082] The difference between this embodiment and embodiment 1 is that, in terms of mass percentage, the low-temperature resistant auxiliary agent includes: 30 parts of trehalose, 20 parts of arachidonic acid, and 60 parts of proline.

[0083] Example 4 Cultivation of low temperature resistant Babylonia

[0084] The low-temperature-resistant Dongfeng snails selected and bred in Example 1 were used for semi-closed circulating water culture. The cement pool has a specification of 2.5 meters × 6.0 meters × 0.5 meters. The water inlet pipe and the air pipe are respectively set at the two ends of the upper part of the cement pool wall. At the same time, two polyethylene ropes are pulled between the two ends of the upper part of the pool wall as the purpose of fixing the air pipe. There are 9 air pipes on the bottom of the pool, each of which is 1.5 to 2.0 meters long, and the bottom of the pool is inclined toward the drain. The bottom structure of the pool is first paved with a layer of partitions with a height of 10 cm with a square plastic board 20 cm away from the bottom of the pool, and then 80 mesh sieve silk is spread on the board, and 6 cm thick fine sand disinfected with potassium permanganate is spread on it. An iron frame is set up 3 meters above the ground, and a sunshade net is laid on the top. The surroundings of the breeding site are surrounded by a sunshade net, and the height is closed with the top seam. The water for breeding is drawn from the sand well in the intertidal zone of the sea area. After settling for 24 hours, it is directly cultivated with running water. Each pool is separately provided with a circulation device, which can control the water temperature to be kept at 14 to 16 ° C. The stocking density of Babylonia snails is 1000 / m 2 , install a 60-mesh silk water inlet bag at the water inlet of the equipment to reduce the entry of harmful organisms into the breeding pond. Feed blue round scads once every two days, accounting for 10-12% of the weight of the snails, and add cold-resistant additives to the bait each time the bait is fed, with the dosage of 1.5 grams of cold-resistant additives per kilogram of bait.

[0085] After 13 months of breeding, the results were: the survival rate of the Dongfeng snails was 83.7%, the specifications of the Dongfeng snails were 45 to 55 heads per kilogram, and the output of Dongfeng snails was 236 kilograms.

[0086] Example 5 Cultivation of low temperature resistant Babylonia

[0087] The difference between this embodiment and embodiment 4 is that the low-temperature-resistant Babylonia snails bred in embodiment 2 are used.

[0088] After 13 months of breeding, the results were: the survival rate of the Dongfeng snails was 86.9%, the specifications of the Dongfeng snails were 45 to 55 heads per kilogram, and the output of Dongfeng snails was 245 kilograms.

[0089] Example 6 Cultivation of low temperature resistant Babylonia

[0090] The difference between this embodiment and embodiment 4 is that the low-temperature-resistant Babylonia snails bred in embodiment 3 are used.

[0091] After 13 months of breeding, the results were: the survival rate of the Dongfeng snails was 80.4%, the specifications of the Dongfeng snails were 45 to 55 heads per kilogram, and the output of Dongfeng snails was 231 kilograms.

[0092] Example 7

[0093] The difference between this embodiment and embodiment 4 is that no low temperature resistant additive is used in the breeding process.

[0094] After 13 months of breeding, the results were: the survival rate of the Dongfeng snails was 61.3%, the specifications of the Dongfeng snails were 50 to 60 heads per kilogram, and the output of Dongfeng snails was 162 kilograms.

[0095] Example 8

[0096] The difference between this embodiment and embodiment 4 is that no low-temperature resistant additive is used in the breeding process, and the low-temperature resistant Babylonia snails bred in embodiment 2 are used.

[0097] After 13 months of breeding, the results were: the survival rate of the Dongfeng snails was 57.4%, the specifications of the Dongfeng snails were 50 to 60 heads per kilogram, and the output of Dongfeng snails was 152 kilograms.

[0098] Example 9

[0099] The difference between this embodiment and embodiment 4 is that no low-temperature resistant additive is used in the breeding process, and the low-temperature resistant Babylonia snails bred in embodiment 3 are used.

[0100] After 13 months of breeding, the results were: the survival rate of the Dongfeng snails was 60.8%, the specifications of the Dongfeng snails were 50 to 60 heads per kilogram, and the output of Dongfeng snails was 157 kilograms.

[0101] Comparative Example 1

[0102] The difference between this comparative example and Example 4 is that the low-temperature-resistant Babylonia snails bred in Example 1 are replaced by the Babylonia snails obtained by breeding without using low-temperature-resistant additives during the breeding process in Example 1.

[0103] After 13 months of breeding, the result was: all the snails died.

[0104] Comparative Example 2

[0105] The difference between this embodiment and embodiment 4 is that the unbred Babylonia areolata in step (1) and 1) of embodiment 1 is used to replace the low-temperature-resistant Babylonia bred in embodiment 1.

[0106] After 13 months of breeding, the results were: the survival rate of the Dongfeng snails was 13.7%, the size of the Dongfeng snails was 70 to 80 heads per kilogram, and the output of Dongfeng snails was 24.9 kilograms.

[0107] Comparative Example 3

[0108] The difference between this comparative example and Example 4 is that no low-temperature resistant additive is used; the unbred Babylonia areolata in step (1) and 1) of Example 1 is used to replace the low-temperature resistant Babylonia bred in Example 1.

[0109] After 13 months of breeding, the results were: the survival rate of the Dongfeng snails was 5.2%, the size of the Dongfeng snails was 80 to 90 heads per kilogram, and the output of Dongfeng snails was 8.8 kilograms.

[0110] The foregoing examples are merely illustrative and are used to explain some features of the method of the present invention. The appended claims are intended to require the widest possible range that can be imagined, and the embodiments presented herein are demonstrated by the applicant's actual test results. Therefore, the applicant's intention is that the appended claims are not limited by the selection of examples that illustrate the features of the present invention. Some numerical ranges used in the claims also include sub-ranges therein, and changes in these ranges should also be interpreted as being covered by the appended claims where possible.

Claims

1. A method for breeding low-temperature resistant Babylonia natans, characterized in that: The following steps are involved: S1. The first generation of low temperature tolerant basic population: The first stage is cooling the larval stage: During the early, middle and late larval stages, the aquaculture water is cooled at least once to 16-18°C. Each cooling is maintained for 6-24 hours before being warmed up to normal temperature. The second stage is cooling during the intermediate cultivation period: During the cultivation of juvenile Dongfeng snails, the temperature is lowered at least three times. The aquaculture water body is cooled to 16-18℃ for the first time, 14-16℃ for the second time, and 12-14℃ for the third and subsequent times. Each cooling is maintained for 12-24 hours before the temperature is raised to normal temperature. The third stage is cooling during the grow-out period: During the grow-out period, the water temperature is cooled several times, starting from 16-18°C, and the water temperature gradually decreases. The last cooling is reduced to 8-10°C, and each cooling is maintained for 24-48 hours before the temperature is raised to normal temperature. Feed the first generation of low-temperature resistant basic population during the breeding period; after the third stage of the growth period, the top 40-70% of the Babylonia snails are selected based on body weight to obtain the first generation of low-temperature resistant basic population; S2. Basic population mating: The first generation of low-temperature resistant basic population is selected for interbreeding and breeding until the gonads of the Babylonia snails mature, mating is performed, and egg sacs are collected; S3. Progeny cultivation: After the oocysts in step S2 are disinfected, they are incubated and cultivated; during the cultivation period, the above step S1 is repeated to obtain a second generation breeding cold-resistant base population; S4. After the second generation of low-temperature resistant basic population is bred, steps S2 and S3 are repeated, and the water temperature is maintained at 10 to 18°C ​​for breeding, so as to obtain low-temperature resistant Babylonia snails; In the steps S1 to S4, a low-temperature resistant additive is added to the aquaculture water; Calculated by weight, the low-temperature resistant auxiliary agent includes: 20 to 30 parts of trehalose, 20 to 30 parts of arachidonic acid, and 40 to 60 parts of proline.

2. The breeding method according to claim 1, characterized in that: In step S1, at least one of the following (a) to (c) is selected: (a) When cooling the larvae in the first stage, the interval between each cooling is 2 to 6 days; (b) During the second stage of the intermediate incubation period, the temperature is lowered every 6 to 12 days; (c) During the third stage of the growing period, the temperature is lowered with an interval of 20 to 40 days between each cooling.

3. The breeding method according to claim 1, characterized in that: In step S1, at least one of the following (d) to (f) is selected: (d) When the temperature drops during the first stage of larval stage, the amount of low temperature resistant additive added is 0.5-3 ppm; (e) During the second stage of the intermediate incubation period, when the temperature drops, the amount of low-temperature resistant additive added is 0.1 to 2 grams per kilogram of bait; (f) When the temperature drops during the third stage of the culture period, the amount of cold-resistant additive added is 0.1 to 2 grams per kilogram of bait.

4. The breeding method according to claim 1, characterized in that: In the step S1, when cooling down during the third stage of the cultivation period, the water temperature is lowered twice as one small cycle, and the water temperature is gradually lowered between each small cycle, which lasts for 4 to 6 small cycles.

5. The breeding method according to claim 1, characterized in that: In step S1, the bait is selected from one or more of clams, mussels, oysters, scallop meat, sardines, and blue round trevally.

6. The breeding method according to claim 1, characterized in that: In step S3, when step S1 is repeated during the incubation period, at least one of the following (g) to (i) is selected: (g) When cooling the larvae in the first stage, the interval between each cooling is 1 to 4 days; (h) During the second stage of the intermediate incubation period, the temperature is lowered every 3 to 8 days; (i) During the third stage of the growing period, the temperature is lowered with an interval of 5 to 20 days between each cooling.

7. The breeding method according to claim 1, characterized in that: In step S2, enhanced ripening is carried out during the breeding period to accelerate the development and maturation of the gonads of the Babylonia snails.

8. The breeding method according to claim 1, characterized in that: In the steps S1 to S4, the salinity is controlled at 28 to 30 ppt, the dissolved oxygen is ≥ 5 mg / L, and the pH is 7.9 to 8.

1.

9. A method for cultivating Babylonia natans at low temperature, characterized in that: During the cultivation process of the low-temperature-resistant Babylonia snails bred by the breeding method according to any one of claims 1 to 8, a low-temperature-resistant additive is added.

10. The breeding method according to claim 9, characterized in that: The amount of the low temperature resistant additive added is 0.01-0.02% of the total weight of the babylonia.

11. The breeding method according to claim 9, characterized in that: The temperature during the breeding process is 8-18℃.