A protein separation device

Abstract

The present invention relates to the technical field of protein separation, and specifically to a protein separation device, which includes a precipitation mixing tank. A rotary power mechanism is arranged between the top and the lower side of the precipitation mixing tank. The bottom of the precipitation mixing tank is rotatably communicated with a membrane separation tank. The membrane separation tank is stationary relative to the precipitation mixing tank and receives the protein processed inside the precipitation mixing tank. A vibrating multi-stage filter membrane mechanism is arranged inside the membrane separation tank. A circulating lifting assembly is arranged between the lower part of the precipitation mixing tank and the outer wall of the membrane separation tank. A simple refrigeration box is fixedly installed on the outer wall of the membrane separation tank. A simple refrigeration system is arranged inside the simple refrigeration box. The temperature and pressure component in the simple refrigeration system is connected to the circulating lifting assembly. During the separation process of this device, it controls the real-time and uniform transmission and diffusion of proteins, and adopts multi-stage vibrating membrane filtration separation to ensure the filtration purity. At the same time, it effectively converts and utilizes kinetic energy, improves energy utilization efficiency, and reduces processing costs.

Description

Technical Field

[0001] The present invention relates to the technical field of protein separation, and specifically to a protein separation device. Background Art

[0002] Protein separation is an important technique in biochemistry and molecular biology, used to isolate and purify specific proteins from complex biological samples. This process is crucial for studying the structure, function, and role of proteins in various biological processes. The following are some commonly used protein separation methods: Centrifugation: Utilizing the differences in protein molecular weight and density, different proteins are separated by centrifugal force.

[0003] Chromatography:

[0004] Gel filtration chromatography: Separates proteins according to molecular size;

[0005] Ion exchange chromatography: Separates based on the differences in protein surface charge;

[0006] Affinity chromatography: Separates by utilizing the specific binding of proteins to specific ligands;

[0007] Electrophoresis techniques:

[0008] SDS-PAGE (Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis): Separates according to protein molecular weight;

[0009] Isoelectric focusing: Separates based on the differences in protein isoelectric points;

[0010] Two-dimensional electrophoresis: Combines isoelectric focusing and SDS-PAGE, and can simultaneously separate complex protein mixtures. Precipitation method:

[0011] Uses methods such as salting out, organic solvents, or heat treatment to precipitate the target protein, thereby separating it from other components.

[0012] Membrane separation technology:

[0013] Such as ultrafiltration and microfiltration, which use membranes with different pore sizes to separate protein molecules of different sizes.

[0014] Chromatography - mass spectrometry coupling technology:

[0015] Combining high-performance liquid chromatography (HPLC) and mass spectrometry (MS) technologies can achieve highly sensitive and specific protein separation and identification.

[0016] Selecting the appropriate protein separation method depends on multiple factors, including the properties of the target protein, the complexity of the sample, the required purity, and the requirements of subsequent analysis, etc. Generally, in order to obtain a highly pure protein sample, it is necessary to combine multiple separation techniques.

[0017] The existing patent application number is: 2023112823015, which discloses a protein separation and purification device, including an outer barrel. Inside the outer barrel, a stirring barrel is installed. At the bottom of the stirring barrel, there is a first discharge port. Inside the first discharge port, a solenoid valve is arranged. A filter membrane plate is slidably connected inside the outer barrel. The filter membrane plate is located below the first discharge port. A connecting shaft is rotatably installed inside the outer barrel, and a water wheel is fixedly installed on the connecting shaft. After putting the material and the precipitant into the stirring barrel, the material and the precipitant are evenly stirred and mixed. Subsequently, the solenoid valve inside the first discharge port is opened, so that the precipitated protein will stay above the filter membrane plate, and the water liquid will flow downward through the filter membrane plate. Utilizing the gravity generated when the water liquid descends, the water wheel is driven to rotate, the connecting shaft is driven to rotate, and the cam is driven to rotate, which can collide with the filter membrane plate to make the filter membrane plate vibrate, facilitating the separation of the precipitated protein from the water liquid;

[0018] After analyzing the above patent, it can be seen that there are the following problems: First, when the protein and the precipitant are initially input, they are only simply put into the stirring barrel, and then the subsequent mixing is carried out evenly by using the stirring structure. That is, it cannot ensure that the protein and the precipitant put into the barrel are in a uniform state, which will affect the subsequent mixing speed and efficiency to a certain extent;

[0019] Secondly, when the protein is separated and processed, in order to maintain its activity, it needs to be placed in a low-temperature environment. However, for the temperature inside the above-mentioned stirring barrel and some existing separation devices, an additional power-driven refrigeration device is required to maintain real-time control, which will increase the operation cost of the equipment;

[0020] Finally, for the existing equipment, when multiple separation technologies are combined and used, for each piece of equipment involved in each separation technology, an additional corresponding power device or energy is required to drive the operation, and the energy-saving operation cannot be effectively controlled, resulting in a substantial increase in the processing cost;

[0021] Therefore, in view of the above existing problems, the present technical solution proposes a protein separation device. Summary of the Invention

[0022] The purpose of the present invention is to provide a protein separation device to solve the problems raised in the above background technology.

[0023] To achieve the above object, the present invention provides the following technical solutions: A protein separation device, comprising a feed channel and a precipitation mixing tank; The precipitation mixing tank is rotatably connected and installed at the bottom of the feed channel, and is used for uniformly mixing, precipitating and separating the protein and the precipitant input through the feed channel. A group of conical cloth cylinders are arranged in the middle of the precipitation mixing tank. The top of the conical cloth cylinder movably passes through the middle of the top of the precipitation mixing tank and is connected to the bottom end of the feed channel. The protein and the precipitant input through the feed channel are evenly sprayed radially outward through the conical cloth cylinder into the precipitation mixing tank, and then, in cooperation with the rotation of the precipitation mixing tank, when the protein and the precipitant are initially put in, they are input in a uniform form;

[0024] A rotation power mechanism is installed between the top of the precipitation mixing tank and the lower side of the feed channel, and is used to drive the precipitation mixing tank to rotate to mix and react and precipitate the input protein and precipitant. The top of the precipitation mixing tank is fixedly connected to the rotation power mechanism, and the precipitation mixing tank is driven by the rotation power mechanism to rotate synchronously. The bottom of the feed channel is rotationally connected to the rotation power mechanism, and the feed channel is stationary relative to the rotation power mechanism;

[0025] A membrane separation tank is rotatably connected and arranged at the bottom of the precipitation mixing tank. The membrane separation tank is stationary relative to the precipitation mixing tank and receives the protein processed inside the precipitation mixing tank, and performs multi-stage membrane filtration separation on it. A discharge port is opened at the bottom end of the membrane separation tank, and the protein after membrane separation treatment is output through the discharge port for subsequent process operations. A vibrating multi-stage filter membrane mechanism is arranged inside the membrane separation tank, and the multi-stage filter membrane mechanism is used for performing multiple vibrating filtration separation treatments on the protein. A uniform distribution component is arranged on the upper side inside the membrane separation tank, and the uniform distribution component, in cooperation with the gravity, uniformly diffuses and conveys the protein input from the precipitation mixing tank onto the multi-stage filter membrane mechanism, ensuring that the protein after mixing and precipitation fully falls on the vibrating multi-stage filter membrane mechanism for rapid and efficient filtration treatment;

[0026] A circulating lifting component is arranged between the lower part of the precipitation mixing tank and the outer side wall of the membrane separation tank. The circulating lifting component is synchronously circulated and lifted by the rotation power of the precipitation mixing tank. A vibrating connecting piece is connected to one side of the vibrating multi-stage filter membrane mechanism facing the circulating lifting component. Under the action of the vibrating connecting piece, the vibrating multi-stage filter membrane mechanism is synchronously driven to vibrate at the same frequency, and the protein placed on it is subjected to filter membrane filtration separation;

[0027] The simple refrigeration box is fixedly installed on the outer side wall of the membrane separation box. A simple refrigeration system is arranged inside the simple refrigeration box. The temperature and pressure components in the simple refrigeration system are connected to the circulating lifting component. By utilizing the kinetic energy of the circulating lifting component, the temperature and pressure of the gas are regulated by driving the temperature and pressure components. Then, it operates in cooperation with other components in the simple refrigeration system to regulate the internal temperatures of the feed channel, the precipitation mixing box, and the membrane separation box, keeping the internal temperatures of the feed channel, the precipitation mixing box, and the membrane separation box in a low-temperature state, that is, controlling the proteins to be in an active state for separation and processing. By effectively utilizing the kinetic energy of the rotary power mechanism, compared with the existing refrigeration system, the utilization rate of kinetic energy is improved to a certain extent, and the processing cost of this equipment is reduced.

[0028] Compared with the prior art, the beneficial effects of the present invention are as follows: By driving the precipitation mixing box to rotate and operate through the connection structure of a servo motor, bevel gear I, and bevel gear II, and then arranging a conical cloth cylinder and a discharge hole communicated with the feed channel inside the precipitation mixing box, it is realized that when proteins and precipitants are initially input, they are diffused into the precipitation mixing box in a uniform manner, ensuring the uniformity at the beginning of separation. And a cleaning component and a spiral output knife are arranged inside the precipitation mixing box. In cooperation with the rotation of the precipitation mixing box, the output of proteins is accelerated and the cleanliness inside the precipitation mixing box is improved.

[0029] By arranging a uniform distribution component at the top inside the membrane separation box, in cooperation with the structural distribution of the output pipe, the rotary lifting pressure ring, and the conical transmission pipe, it is controlled that the falling proteins can be evenly, quickly, and stably distributed on the first-stage filter membrane for membrane filtration separation. And through double separation by the first-stage filter membrane and the second-stage filter membrane, the filtration purity can be greatly improved.

[0030] By arranging a separate servo motor to drive the rotation of the precipitation mixing box, then arranging a rotary lifting pressure ring on the precipitation mixing box that rotates with the precipitation mixing box, and then cooperating with the circulating lifting component arranged on one side of the membrane separation box, the circulating lifting component is driven to move up and down by the rotary lifting pressure ring, and then the first-stage filter membrane and the second-stage filter membrane are driven for vibratory filtration separation. And under the connection of the piston rod, the simple compressor is driven to operate. Thus, the full conversion and utilization of the kinetic energy of the servo motor operation are realized, and the utilization rate of energy of this equipment is greatly improved to a certain extent, reducing the overall processing cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 is the first perspective structural schematic diagram of the present invention;

[0032] Figure 2 is the second perspective structural schematic diagram of the present invention;

[0033] Figure 3 is the front view structural schematic diagram of the present invention;

[0034] Figure 4 Schematic top view structure diagram of the present invention;

[0035] Figure 5 Schematic bottom view structure diagram of the present invention;

[0036] Figure 6 Schematic side view structure diagram of the present invention;

[0037] Figure 7 For the present invention's Figure 3 Enlarged structure diagram of A therein;

[0038] Figure 8 For the present invention's Figure 2 Enlarged structure diagram of B therein;

[0039] Figure 9 For the present invention's Figure 1 Enlarged structure diagram of C therein;

[0040] Figure 10 Schematic internal structure diagram of the simple refrigeration box of the present invention;

[0041] Figure 11 Schematic flow diagram of the simple refrigeration system of the present invention.

[0042] In the figure: feeding channel 10, precipitation mixing tank 11, membrane separation tank 12, servo motor 13, fixed bracket 14, simple refrigeration box 15, connecting cylinder 16, bevel gear I 17, bevel gear II 18, positioning collar 19, conical cloth cylinder 20, discharge hole 21, connecting rod 22, scraper 23, output pipe 24, spiral output knife 25, rotating lifting pressure ring 26, transition channel 27, conical transmission pipe 28, upper V-shaped diffusion mesh plate 29, lower V-shaped diffusion mesh plate 30, grade I filter membrane 31, grade II filter membrane 32, vibration tank 33, filter membrane end plate 34, lifting chute 35, lifting slider 36, telescopic cylinder ring 37, lower fixing plate 38, upper fixing plate 39, telescopic rod 40, sleeve spring 41, ball 43, lifting rod 44, piston rod 45, blanking port 46, one-way air inlet 47, evaporator 48, partition board 49, simple compressor 50, condenser 51, expansion valve 52, exhaust port 53, intake transmission pipe 54, refrigerant return pipe 55, control valve 56, intake valve port 57, heat conduction plate layer 58, refrigeration chamber 59. Specific embodiments

[0043] It should be noted that, without conflict, the embodiments in the present invention and the features in the embodiments can be combined with each other.

[0044] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by the terms "center", "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus cannot be construed as a limitation on the present invention. In addition, the terms "first", "second", etc. are only used for descriptive purposes and cannot be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first", "second", etc. may explicitly or implicitly include one or more of such features. In the description of the present invention, unless otherwise specified, the meaning of "a plurality" is two or more.

[0045] In the description of the present invention, it should be noted that unless otherwise clearly specified and defined, the terms "installed", "connected", "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, and can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood through specific situations.

[0046] The present invention will be described in detail below with reference to the drawings and in combination with embodiments.

[0047] Please refer to Figures 1 - 6 , a protein separation device, including a feed channel 10 and a precipitation mixing tank 11; the precipitation mixing tank 11 is rotatably and communicatively installed at the bottom of the feed channel 10, and is used for uniformly mixing and precipitating and separating the protein and the precipitant input through the feed channel 10. A group of conical cloth cylinders 20 are arranged in the middle of the precipitation mixing tank 11. The top of the conical cloth cylinder 20 movably passes through the middle of the top of the precipitation mixing tank 11 and communicates with the bottom end of the feed channel 10. The protein and the precipitant input through the feed channel 10 are evenly sprayed radially outward through the conical cloth cylinder 20 into the precipitation mixing tank 11, and then, in cooperation with the rotation of the precipitation mixing tank 11, when the protein and the precipitant are initially put in, they are input in a uniform form;

[0048] A rotation power mechanism is installed between the top of the precipitation mixing tank 11 and the lower side of the feed channel 10, and is used for driving the precipitation mixing tank 11 to rotate to mix and react and precipitate the input protein and precipitant. The top of the precipitation mixing tank 11 is fixedly connected to the rotation power mechanism, and the precipitation mixing tank 11 rotates synchronously under the drive of the rotation power mechanism. The bottom of the feed channel 10 is rotationally connected to the rotation power mechanism, and the feed channel 10 is stationary relative to the rotation power mechanism;

[0049] The membrane separation tank 12 is rotatably connected and arranged at the bottom of the precipitation mixing tank 11. The membrane separation tank 12 is stationary relative to the precipitation mixing tank 11 and receives the protein processed inside the precipitation mixing tank 11, and performs multi-stage membrane filtration separation on it. A discharge port 46 is opened at the bottom end of the membrane separation tank 12. The protein after membrane separation treatment is output through the discharge port 46 for subsequent process operations. A vibrating multi-stage filter membrane mechanism is arranged inside the membrane separation tank 12. The multi-stage filter membrane mechanism is used for performing multiple vibrating filtration separation treatments on the protein. A uniform distribution component is arranged on the upper side inside the membrane separation tank 12. The uniform distribution component cooperates with the gravity to uniformly diffuse and transport the protein input from the precipitation mixing tank 11 onto the multi-stage filter membrane mechanism, ensuring that the protein after mixing and precipitation fully falls on the vibrating multi-stage filter membrane mechanism for rapid and efficient filtration treatment;

[0050] The circulating lifting component is arranged between the lower part of the precipitation mixing tank 11 and the outer side wall of the membrane separation tank 12. The circulating lifting component is synchronously lifted and lowered by the rotating power of the precipitation mixing tank 11. A vibrating connecting piece is connected to one side of the vibrating multi-stage filter membrane mechanism facing the circulating lifting component. Under the action of the vibrating connecting piece, the vibrating multi-stage filter membrane mechanism is synchronously driven to vibrate at the same frequency, and the protein placed on it is subjected to filter membrane filtration separation;

[0051] The simple refrigeration box 15 is fixedly installed on the outer side wall of the membrane separation tank 12. A simple refrigeration system is arranged inside the simple refrigeration box 15. The temperature and pressure components in the simple refrigeration system are connected to the circulating lifting component. By utilizing the kinetic energy of the circulating lifting component, the temperature and pressure of the gas are driven to be adjusted, and then it is operated in cooperation with other components in the simple refrigeration system to adjust the internal temperatures of the feed channel 10, the precipitation mixing tank 11, and the membrane separation tank 12, keeping the internal temperatures of the feed channel 10, the precipitation mixing tank 11, and the membrane separation tank 12 in a low temperature state, that is, controlling the protein to be in an active state for separation and processing. By effectively utilizing the kinetic energy of the rotating power mechanism, compared with the existing refrigeration system, the utilization rate of the kinetic energy is improved to a certain extent, and the processing cost of this equipment is reduced.

[0052] In the embodiment of the present invention, the feed channel 10 and the precipitation mixing tank 11 are both arranged in a funnel-shaped structure, which is convenient for improving the stability and efficiency when the protein and the precipitant are input, and at the same time improving the centralized output speed;

[0053] A precipitant refers to a substance that can precipitate the target protein from the solution. These precipitants change the environment around the protein and reduce the solubility of the protein, thereby causing it to precipitate; commonly used precipitants include: neutral salts (salting out): ammonium sulfate, sodium sulfate, sodium chloride, etc.; organic solvents: methanol, ethanol, propanol, etc.; non-ionic polymers: polyethylene glycol, dextran; metal ions: zinc ions, copper ions, etc.;

[0054] Among them, after the protein that has undergone membrane filtration treatment inside the membrane separation tank 12 is output through the discharge port 46, in order to improve the purity of protein separation, it can be further purified according to requirements, and various process treatments such as concentration, buffer exchange, desalting, activity detection, and purity detection can be carried out. Which specific process to choose depends on multiple factors, specifically including the final use of the protein, the required purity and concentration, the stability and characteristics of the protein, available equipment and resources, time and cost considerations, etc. Details are not elaborated here;

[0055] Specifically, the rotary power mechanism, the feed channel 10, and the circulating lifting assembly are all positioned and installed on the side wall of the membrane separation tank 12 through the fixed bracket 14 to maintain the stable operation of the cooperation between the above structures.

[0056] In an example of the present invention, cleaning members are symmetrically installed on the outer wall of the lower side circumference of the conical cloth cylinder 20. In cooperation with the rotation of the precipitation mixing tank 11, the inner wall of the precipitation mixing tank 11 is scraped to prevent proteins, precipitants, etc. from adhering to the inner wall of the precipitation mixing tank 11;

[0057] Discharge holes 21 are uniformly arranged on the outer wall of the upper side of the conical cloth cylinder 20, and the proteins and precipitants inside the conical cloth cylinder 20 are uniformly sprayed out through the discharge holes 21;

[0058] The rotary power mechanism includes a bevel gear II 18 sleeved on the outside of the conical cloth cylinder 20 at the upper part of the precipitation mixing tank 11. A connecting cylinder 16 with the bottom end fixed on the top surface of the precipitation mixing tank 11 is connected outward at the bottom of the bevel gear II 18. The connecting cylinder sleeves the conical cloth cylinder 20. A bevel gear I 17 is vertically meshed on one side of the bevel gear II 18. A servo motor 13 is connected to the center of one side of the bevel gear I 17. The outer end of the servo motor 13 is fixed on the outer wall of the membrane separation tank 12 through the fixed bracket 14. That is, when the servo motor 13 is started, the bevel gear I 17 is driven to control the rotation of the bevel gear II 18. Then, under the connection of the connecting cylinder 16, the precipitation mixing tank 11 is driven to rotate. At this time, the feed channel 10, the conical cloth cylinder 20, and the cleaning member at the bottom end of the conical cloth cylinder 20 are all in a static state, thereby realizing the independent rotation of the precipitation mixing tank 11;

[0059] At the same time, a positioning collar 19 is positioned and sleeved outside the connecting cylinder 16. One side of the positioning collar 19 is fixed on the outer wall of the membrane separation tank 12 through the fixed bracket 14. Under the limitation of the fixed bracket 14 and the positioning collar 19, the precipitation mixing tank 11 is kept suspended and rotating above the membrane separation tank 12.

[0060] Specifically, refer to Figure 9, a discharge pipe 24 is connected to the bottom end of the precipitation mixing tank 11. A set of spiral discharge blades 25 is vertically arranged inside the discharge pipe 24. The top of the spiral discharge blades 25 is fixedly connected to the bottom end of the conical cloth cylinder 20. The bottom end of the conical cloth cylinder 20 is set to be closed. Under the rotation of the precipitation mixing tank 11, the discharge pipe 24 is controlled to rotate along the outer side of the spiral discharge blades 25. Then, by using the spiral structure of the spiral discharge blades 25, the output of the precipitated protein is accelerated. A solenoid valve is arranged on the lower side of the discharge pipe 24 for remotely controlling the output of the protein;

[0061] The cleaning member includes a fixing ring fixed to the lower side of the conical cloth cylinder 20. Two inclined downward connecting rods 22 are symmetrically installed on both sides of the fixing ring. A scraper 23 in contact with the inner wall of the precipitation mixing tank 11 is installed at the end of the connecting rod 22. Under the rotation of the precipitation mixing tank 11, the inner wall of the precipitation mixing tank 11 is controlled to continuously contact with the scraper 23, thereby scraping the inner wall of the precipitation mixing tank 11, that is, reducing the temperature of the protein adhering to the precipitation mixing tank 11.

[0062] As a preferred embodiment of the present invention, a transition channel 27 with an inverted funnel-shaped structure is rotatably connected to the bottom of the end of the discharge pipe 24. The bottom of the transition channel 27 is connected to a membrane separation tank 12 with a rectangular structure. The bottom end of the transition channel 27 is connected to a conical transmission pipe 28 arranged inside the top of the membrane separation tank 12. The bottom of the conical transmission pipe 28 is connected to a uniform distribution component. The protein is concentrated through the discharge pipe 24, diffused by the rotating lifting pressure ring 26, and concentrated by the conical transmission pipe 28, and is concentrated and diffused repeatedly, changing the force received during the flow of the protein, so that it can fall in a uniformly mixed manner;

[0063] The uniform distribution component uniformly receives the protein falling in the conical transmission pipe 28, and then uniformly falls on the vibrating multi-stage filter membrane mechanism in two times for filtration and separation processing;

[0064] The uniform distribution component includes an upper V-shaped diffusion mesh plate 29 distributed below the conical transmission pipe 28. The upper V-shaped diffusion mesh plate 29 diffuses the protein concentrated and falling in the conical transmission pipe 28 towards both sides. At the same time, by using the mesh structure of the upper V-shaped diffusion mesh plate 29, the protein uniformly flows downward along the upper V-shaped diffusion mesh plate 29. A set of lower V-shaped diffusion mesh plates 30 perpendicular to the upper V-shaped diffusion mesh plate 29 is arranged below the upper V-shaped diffusion mesh plate 29. The protein falling along the upper V-shaped diffusion mesh plate 29 is further diffused by the lower V-shaped diffusion mesh plate 30 and fully and uniformly falls on the vibrating multi-stage filter membrane mechanism for automatic filtration and separation.

[0065] As a preferred embodiment of the present invention, the vibrating multi-stage filter membrane mechanism includes two sets of stage-I filter membranes 31 and stage-II filter membranes 32 that vibrate vertically and circularly inside the membrane separation tank 12. The stage-I filter membranes 31 and the stage-II filter membranes 32 have the same structure and are both used for membrane filtration and separation processing of the falling and contacting protein;

[0066] Taking the Grade-I filter membrane 31 as an example, it is described as follows: Inside the Grade-I filter membrane 31, a filter membrane and a reverse osmosis membrane are sequentially arranged from top to bottom. The filter membrane further includes a microfiltration membrane, an ultrafiltration membrane, and a nanofiltration membrane distributed from top to bottom. Microfiltration membrane: The pore size is about 0.1 - 10 μm, used for separating large particles and bacteria. Ultrafiltration membrane: The pore size is about 1 - 100 nm, used for separating proteins and macromolecules. Nanofiltration membrane: The pore size is about 1 nm, capable of separating small molecules and divalent ions. Reverse osmosis membrane: The pore size < 1 nm, capable of separating monovalent ions and water. That is, by passing proteins through the above-mentioned filter membranes, they can be fully and accurately separated and processed.

[0067] As a preferred embodiment of the present invention, referring to Figure 8 , the vibration connecting member includes filter membrane end plates 34 installed on the outer edges of the Grade-I filter membrane 31 and the Grade-II filter membrane 32. Vibration grooves 33 are provided on the inner walls of the membrane separation tank 12 corresponding to the two filter membrane end plates 34 at both ends. The filter membrane end plates 34 move vertically along the vibration grooves 33. A lifting slider 36 is installed in the middle of one of the filter membrane end plates 34. An internally and externally penetrating lifting chute 35 is provided on the inner wall of the vibration groove 33 corresponding to the lifting slider 36. The lifting slider 36 moves up and down along the inside of the lifting chute 35. The outer end of the lifting slider 36 is connected to the circulating lifting assembly, thereby driving the Grade-I filter membrane 31 and the Grade-I filter membrane 31 to circulate and vibrate inside the membrane separation tank 12, that is, improving the filtration efficiency of proteins.

[0068] Specifically, a sealing plate is installed on the lifting slider 36 placed outside the lifting chute 35. The sealing plate is in sealed sliding contact with the outer wall of the membrane separation tank 12 outside the lifting chute 35. When the lifting slider 36 moves up and down, it synchronously drives the sealing plate to move along the outside of the lifting chute 35 and seals the lifting chute 35, preventing the proteins inside the membrane separation tank 12 from leaking out along the lifting chute 35.

[0069] As a preferred embodiment of the present invention, the cyclic lifting assembly includes lifting rods 44 fixedly connected to the outer parts of the ends of two lifting sliders 36. The bottom end of the lifting rod 44 is fixedly connected to the temperature and pressure member. The top end of the lifting rod 44 is fixedly connected with a telescopic rod 40. Both ends of the telescopic rod 40 movably pass through a lower fixing plate 38 and an upper fixing plate 39. At the positions corresponding to the telescopic rod 40 on the lower fixing plate 38 and the upper fixing plate 39, telescopic cylinder rings 37 are installed. The telescopic rod 40 is vertically slid inside the telescopic cylinder ring 37. A sleeve spring 41 is sleeved on the telescopic rod 40 between the lower fixing plate 38 and the upper fixing plate 39. A ball 43 is rotatably connected to the top end of the telescopic rod 40. The top end of the ball 43 is in rolling contact with a rotating lifting pressure ring 26. The inner side of the rotating lifting pressure ring 26 is fixedly installed on the outer wall of the lower part of the precipitation mixing tank 11. The rotating lifting pressure ring 26 rotates synchronously with the precipitation mixing tank 11. The bottom end of the rotating lifting pressure ring 26 has a chamfer structure. By using the elastic effect of the sleeve spring 41 and cooperating with the rolling connection between the ball 43 and the bottom end of the rotating lifting pressure ring 26, the telescopic rod 40 is controlled to cyclically lift between the lower fixing plate 38 and the upper fixing plate 39, and then drive the lifting rod 44 to move up and down. Furthermore, according to the connection of the lifting slider 36, the primary filter membrane 31 and the secondary filter membrane 32 are driven to vibrate and operate, and cyclic lifting power is provided for the temperature and pressure member to drive it to operate smoothly.

[0070] Specifically, one side of each of the lower fixing plate 38 and the upper fixing plate 39 is fixedly connected to the outer wall of the membrane separation tank 12 through a fixing bracket 14 to maintain the stable operation of the lower fixing plate 38 and the upper fixing plate 39.

[0071] As a preferred embodiment of the present invention, refer to Figure 7 、 Figure 10 、 Figure 11, the simple refrigeration system includes a partition plate 49 fixed inside the simple refrigeration box 15. On both sides inside the simple refrigeration box 15, a condenser 51 and an evaporator 48 are respectively installed. In the middle of the upper fixing plate 39 between the evaporator 48 and the condenser 51, a temperature and pressure component is installed and set as a simple compressor 50. Inside the simple compressor 50, a power component is provided. The top of the power component is connected to the bottom of the lifting rod 44 through a piston rod 45. By means of the cyclic lifting of the lifting rod 44 and the connection of the piston rod 45, the power component is driven to operate cyclically to control the operation of the simple compressor 50. Inside the partition plate 49 below the telescopic rod 40, a group of expansion valves 52 is provided. Both sides of the expansion valve 52 are respectively connected to the lower parts of the condenser 51 and the evaporator 48 through refrigerant return pipes 55, and control valves 56 are also provided on the refrigerant return pipes 55. The upper part of the side wall of the condenser 51 is communicated with an exhaust port 53 whose end extends to the outside of the simple refrigeration box 15. The top of the evaporator 48 is communicated with an intake transmission pipe 54. The end of the intake transmission pipe 54 is communicated with an intake valve port 57 arranged in a refrigeration chamber 59 opened in the side wall inside the membrane separation box 12. The bottom of the refrigeration chamber 59 is downwardly communicated with a one-way intake port 47 arranged at the bottom of the membrane separation box 12. The one-way intake port 47 is used for external gas to enter the refrigeration chamber 59 for use. The high-temperature gas inside the refrigeration chamber 59 is output through the intake transmission pipe 54, and then the temperature inside the refrigeration chamber 59 is gradually reduced. The outside of the refrigeration chamber 59 is connected with a heat conduction plate layer 58 arranged on the inner walls of the feed channel 10, the precipitation mixing box 11, and the membrane separation box 12. As the temperature inside the refrigeration chamber 59 decreases, the low-temperature transfer of the heat conduction plate layer 58 is utilized until the temperatures inside the feed channel 10, the precipitation mixing box 11, and the membrane separation box 12 are all reduced to the magnitude for safe protein separation. At the same time, under the continuous operation state of the precipitation mixing box 11, the inside of the refrigeration chamber 59 is continuously maintained, and the temperatures inside the feed channel 10, the precipitation mixing box 11, and the membrane separation box 12 are synchronously maintained. That is, by using the rotational kinetic energy of the precipitation mixing box 11 to drive the operation of the simple compressor 50, compared with the power required for the operation of the simple compressor 50 in the prior art, a certain degree of energy saving can be achieved;

[0072] Specifically, the power component includes a compression chamber opened inside the simple compressor 50. A piston rod is movably arranged inside the compression chamber. The top of the piston rod is connected to the bottom of the piston rod 45. On both sides of the bottom of the compression chamber, they extend outward and are connected to the evaporator 48 and the condenser 51 through connecting pipes. Control valves 56 are arranged on both the evaporator 48 and the condenser 51. The low-temperature and low-pressure liquid refrigerant is placed in the evaporator 48 for evaporation and heat absorption. Then, under the connection of the intake transmission pipe 54, heat absorption is carried out in the refrigeration chamber 59 to reduce its temperature. Then, the evaporated refrigerant is converted into low-temperature and low-pressure gaseous state, and then input into the compression chamber in the compressor for compression, and then forms high-temperature and high-pressure gaseous state. Then, it is transferred to the condenser 51 through the connecting pipe again for condensation, and is converted into high-temperature and high-pressure liquid state, and releases heat. At this time, the released heat is discharged out of the simple refrigerator 15 along the exhaust port 53. The high-temperature and high-pressure liquid refrigerant is transferred to the expansion valve 52 again for expansion to form low-temperature and low-pressure liquid state, and then flows back to the evaporator 48 through the refrigerant return pipe 55 for reuse. In this way, the continuous low-temperature control of the temperature inside the refrigeration chamber 59 is realized through repeated cycles;

[0073] It should be noted that the above-mentioned evaporator 48, simple compressor 50, condenser 51, and expansion valve 52 all adopt simple structures. At the same time, they are small in volume and are convenient for centralized and compact installation inside the simple refrigerator 15. At the same time, the structures cooperate closely to control the constancy of the low temperature inside the feed channel 10, precipitation mixing tank 11, and membrane separation tank 12 in real time;

[0074] Regarding the specific structures and operating principles of the evaporator 48, condenser 51, expansion valve 52, etc., they all belong to the prior art and will not be elaborated here.

[0075] The working principle of the present invention is as follows: At the idle position of the device, all the above-mentioned driving components, which refer to power components, electrical components and the adapted power supply, are connected by wires, and the electrical connection is completed according to the sequence of operation among the electrical components. The detailed connection means are well-known techniques in the art. The following mainly introduces the working principle and process, and will not explain the electrical control. During operation, the protein to be separated diffuses evenly along the feeding channel 10 through the discharge holes 21 on the conical cloth cylinder 20 into the sedimentation mixing tank 11. At this time, the servo motor 13 is started to drive the bevel gear I 17 and the bevel gear II 18 to rotate. Then, under the connection of the connecting cylinder 16, the sedimentation mixing tank 11 is driven to rotate. Then, the protein and the precipitant are mixed and reacted in the sedimentation mixing tank 11 to precipitate. After a certain reaction time, the solenoid valve on the output pipe 24 is opened, and the protein falls along the conical transfer pipe 28 onto the upper V-shaped diffusion mesh plate 29, and then is evenly distributed on the first-stage filter membrane 31 through the lower V-shaped diffusion mesh plate 30. The first-stage filter membrane 31 is used for membrane filtration separation. At this time, with the rotation of the sedimentation mixing tank 11, under the rolling of the rotary lifting pressure ring 26 and the ball 43, the telescopic rod 40 is controlled to cycle up and down. Then, under the connection of the lifting slider 36, the first-stage filter membrane 31 and the second-stage filter membrane 32 are driven to cycle and vibrate inside the membrane separation tank 12, so as to perform multi-stage vibration filtration separation on the falling protein. At the same time, with the cyclic up and down movement of the lifting rod 44, the piston rod inside the simple compressor 50 is driven to continuously move up and down, so as to compress the input gaseous refrigerant. Then, with the cooperation of the evaporator 48, the condenser 51 and the expansion valve 52 arranged inside the simple refrigeration box 15, the temperature inside the refrigeration chamber 59 is controlled to be in a low-temperature state, and through the transfer of the heat conduction plate layer 58, the feeding channel 10, the sedimentation mixing tank 11 and the membrane separation tank 12 are controlled to be in a low-temperature state for the safe processing of the protein in real time.

[0076] The above has made a detailed description of the preferred embodiment of the present patent. However, the present patent is not limited to the above embodiment. Within the scope of knowledge possessed by those of ordinary skill in the art, various changes can be made without departing from the purpose of the present patent.

Claims

1. A protein separation device, characterized in that, It includes a feed channel (10) and a precipitation mixing tank (11); the precipitation mixing tank (11) is rotatably and communicatively installed at the bottom of the feed channel (10). A group of conical cloth cylinders (20) are arranged in the middle of the precipitation mixing tank (11). The top of the conical cloth cylinder (20) movably passes through the middle of the top of the precipitation mixing tank (11) and is communicated with the bottom end of the feed channel (10). The protein and precipitant input through the feed channel (10) are evenly sprayed radially outward through the conical cloth cylinder (20) into the precipitation mixing tank (11). A rotary power mechanism is installed between the top of the precipitation mixing tank (11) and the lower side of the feed channel (10). The top of the precipitation mixing tank (11) is fixedly connected to the rotary power mechanism. The precipitation mixing tank (11) is driven by the rotary power mechanism to rotate synchronously. The bottom of the feed channel (10) is rotatably connected to the rotary power mechanism, and the feed channel (10) is stationary relative to the rotary power mechanism. A membrane separation tank (12) is rotatably and communicatively arranged at the bottom of the precipitation mixing tank (11). The membrane separation tank (12) is stationary relative to the precipitation mixing tank (11) and receives the protein processed inside the precipitation mixing tank (11). A discharge port (46) is opened at the bottom end of the membrane separation tank (12). A vibrating multi-stage filter membrane mechanism is arranged inside the membrane separation tank (12). The multi-stage filter membrane mechanism is used for performing multiple vibrating filtration separation treatments on the protein. A uniform distribution component is arranged on the upper side inside the membrane separation tank (12). The uniform distribution component cooperates with the gravity to evenly diffuse and transport the protein input from the precipitation mixing tank (11) onto the multi-stage filter membrane mechanism. A circulating lifting component is arranged between the lower part of the precipitation mixing tank (11) and the outer wall of the membrane separation tank (12). A vibrating connecting piece is connected to one side of the vibrating multi-stage filter membrane mechanism facing the circulating lifting component. A simple refrigeration box (15) is fixedly installed on the outer wall of the membrane separation tank (12). A simple refrigeration system is arranged inside the simple refrigeration box (15). The temperature and pressure component in the simple refrigeration system is connected to the circulating lifting component. The circulating lifting component includes a lifting rod (44) fixedly connected to the outside of the ends of two lifting sliders (36). The bottom end of the lifting rod (44) is fixedly connected to the temperature and pressure component. The top end of the lifting rod (44) is fixedly connected to a telescopic rod (40). The two ends of the telescopic rod (40) movably pass through a lower fixing plate (38) and an upper fixing plate (39). Telescopic cylinder rings (37) are installed at the positions corresponding to the telescopic rod (40) on the lower fixing plate (38) and the upper fixing plate (39). The telescopic rod (40) is vertically slidably arranged inside the telescopic cylinder rings (37). A sleeve spring (41) is sleeved on the telescopic rod (40) between the lower fixing plate (38) and the upper fixing plate (39). The top end of the telescopic rod (40) is rotatably connected to a ball (43). The top of the ball (43) is in rolling contact with a rotary lifting pressure ring (26). The inner side of the rotary lifting pressure ring (26) is fixedly installed on the outer wall of the lower part of the precipitation mixing tank (11). The bottom end of the rotary lifting pressure ring (26) is cut into an inclined surface structure.

2. The protein separation device according to claim 1, characterized in that, A cleaning member is symmetrically installed on the outer wall of the lower side of the conical fabric cylinder (20), and discharge holes (21) are evenly formed on the outer wall of the upper side of the conical fabric cylinder (20); The cleaning member includes a fixing ring fixed on the lower side of the conical fabric cylinder (20), and two inclined downward connecting rods (22) are symmetrically installed on both sides of the fixing ring. A scraper (23) is installed at the end of the connecting rod (22) and is in contact distribution along the inner wall of the sedimentation mixing tank (11).

3. The protein separation device according to claim 2, characterized in that, The rotary power mechanism includes a bevel gear II (18) sleeved on the outside of the conical fabric cylinder (20) at the upper part of the sedimentation mixing tank (11). A connecting cylinder (16) with the bottom end fixed on the top surface of the sedimentation mixing tank (11) is connected outward at the bottom of the bevel gear II (18). The connecting cylinder sleeves the conical fabric cylinder (20). A bevel gear I (17) is vertically engaged on one side of the bevel gear II (18). A servo motor (13) is connected to the center of one side of the bevel gear I (17). The outer end of the servo motor (13) is fixed on the outer wall of the membrane separation tank (12) through a fixing bracket (14); A positioning collar (19) is positioned and sleeved on the outside of the connecting cylinder (16), and one side of the positioning collar (19) is fixed on the outer wall of the membrane separation tank (12) through a fixing bracket (14).

4. A protein separation device according to claim 3, characterized in that, An output pipe (24) is communicated with the bottom end of the sedimentation mixing tank (11). A group of spiral output blades (25) are vertically arranged inside the output pipe (24). The top of the spiral output blade (25) is fixedly connected to the bottom end of the conical fabric cylinder (20), and the bottom end of the conical fabric cylinder (20) is set to be closed.

5. A protein separation device according to claim 4, characterized in that, The bottom of the output pipe (24) is rotatably communicated with a transition channel (27) in an inverted funnel shape. The bottom of the transition channel (27) is communicated with the membrane separation tank (12) in a rectangular structure. The bottom end of the transition channel (27) is communicated with a conical transmission pipe (28) arranged inside the top of the membrane separation tank (12). The bottom of the conical transmission pipe (28) is communicated with a uniform distribution component; The uniform distribution component includes an upper V-shaped diffusion mesh plate (29) distributed below the conical transmission pipe (28), and a group of lower V-shaped diffusion mesh plates (30) perpendicular to the upper V-shaped diffusion mesh plate (29) are arranged below the upper V-shaped diffusion mesh plate (29).

6. The protein separation device according to claim 5, characterized in that, The vibrating multi-stage filter membrane mechanism includes two groups of grade I filter membranes (31) and grade II filter membranes (32) that vibrate vertically and circularly inside the membrane separation tank (12). The grade I filter membrane (31) and the grade II filter membrane (32) have the same structure; A filter membrane and a reverse osmosis membrane are sequentially arranged from top to bottom inside the grade I filter membrane (31). The filter membrane further includes a microfiltration membrane, an ultrafiltration membrane, and a nanofiltration membrane distributed from top to bottom.

7. The protein separation device according to claim 6, characterized in that, The vibration connecting piece includes a filter membrane end plate (34) installed on the outer edges of the first-stage filter membrane (31) and the second-stage filter membrane (32). Vibration grooves (33) are provided on the inner walls of the membrane separation tank (12) corresponding to the filter membrane end plates (34) at both ends. The filter membrane end plate (34) moves vertically along the vibration grooves (33). A lifting slider (36) is installed in the middle of one of the filter membrane end plates (34). An internally and externally penetrating lifting chute (35) is provided on the inner wall of the vibration groove (33) corresponding to the lifting slider (36). The lifting slider (36) moves up and down inside the lifting chute (35), and the outer end of the lifting slider (36) is connected to a circulating lifting component.

8. A protein separation device according to claim 7, wherein The simple refrigeration system includes a partition plate (49) fixed inside the simple refrigeration box (15). A condenser (51) and an evaporator (48) are respectively installed on both sides inside the simple refrigeration box (15). A temperature and pressure component is installed in the middle of the upper fixing plate (39) between the evaporator (48) and the condenser (51). The temperature and pressure component is set as a simple compressor (50). A power component is provided inside the simple compressor (50). The top of the power component is connected to the bottom of the lifting rod (44) through a piston rod (45). A group of expansion valves (52) are provided inside the partition plate (49) below the telescopic rod (40). Both sides of the expansion valve (52) are respectively connected to the lower parts of the condenser (51) and the evaporator (48) through refrigerant return pipes (55). Control valves (56) are also provided on the refrigerant return pipes (55). An exhaust port (53) with an end extending outside the simple refrigeration box (15) is communicated with the upper part of the side wall of the condenser (51). An intake transmission pipe (54) is communicated with the top of the evaporator (48). The end of the intake transmission pipe (54) is communicated with an intake valve port (57) provided in a refrigeration chamber (59) opened in the side wall of the membrane separation tank (12). The bottom of the refrigeration chamber (59) is communicated downward with a one-way intake port (47) provided at the bottom of the membrane separation tank (12). A heat conduction plate layer (58) is connected to the outside of the refrigeration chamber (59) and is provided in the inner walls of the feed channel (10), the precipitation mixing tank (11), and the membrane separation tank (12).

9. A protein separation device according to claim 8, characterized in that, The power component includes a compression chamber opened inside the simple compressor (50). A piston rod is movably arranged inside the compression chamber. The top of the piston rod is connected to the bottom of the piston rod (45). Both sides of the bottom of the compression chamber are distributed outward and communicated with the evaporator (48) and the condenser (51) through connecting pipes. Control valves (56) are provided on both the evaporator (48) and the condenser (51).

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