Usage method of double-cannula continuous irrigation and negative pressure aspiration liquid culture medium set

Abstract

The present invention discloses a method for using a double cannula continuous flushing negative pressure aspiration liquid culture medium set, which includes preparation work, collection of drainage fluid from an aerobic culture bottle, and collection of drainage fluid from an anaerobic culture bottle. In the preparation work, the patient information is checked and negative pressure tools are prepared. For the collection of drainage fluid from the aerobic culture bottle, the drainage fluid is directly collected using negative pressure. For the collection of drainage fluid from the anaerobic culture bottle, the drainage fluid is first collected using negative pressure, and then the air in the drainage fluid is removed by an air removal component. Advantages: The culture bottle placement box is used to store the culture bottles containing two kinds of culture media, aerobic and anaerobic. The two culture bottles are combined and placed in one box. When sampling, they can be directly opened and used separately, which is simple to operate. Also, the negative pressure connector and the liquid inlet connector are directly connected to the negative pressure tube and the double cannula to achieve negative pressure sampling of the drainage fluid. The extraction can be completed at one time and can be operated by a single person, avoiding the contact between medical staff and the drainage fluid and also preventing the drainage fluid from contacting the outside world, thus preventing occupational exposure and secondary pollution.

Description

Technical Field

[0001] The present invention specifically relates to a method for using a double-tube continuous flushing negative pressure aspiration liquid culture medium set. Background Art

[0002] Infectious pancrealic necrosis (IPN) is a deep soft tissue abscess, formerly known as pancreatic abscess. Currently, with the continuous release of medical evidence, the minimally invasive "step-up" approach has become the preferred treatment strategy for IPN. Double-cannula continuous flushing and negative pressure aspiration is the second step of the "step-up" approach. IPN patients often have repeated high fever clinically. While taking cooling and fever reduction measures, it is necessary to find the cause of the fever. Clinically, blood culture and drainage fluid culture (aerobic and anaerobic) are often used to identify pathogenic bacteria. For the drug resistance of the bacteria cultured from the blood and drainage fluid, targeted antibiotic treatment is used, which is particularly important for controlling the patient's condition.

[0003] The front end of the double cannula is usually placed in one or more abscess cavities, and the tube is exposed for a long time. When the double cannula needs to be retained for drainage fluid culture, the following two methods are commonly used clinically:

[0004] 1. Use a syringe to directly draw out liquid from the end of the tube. However, due to pressure problems, it is difficult to draw out the liquid or the amount retained is far from meeting the standard. Repeated forceful suction not only causes local discomfort to the patient but is also prone to contamination.

[0005] 2. The pus drawn out by negative pressure suction during continuous flushing with double cannulas will be stored in a bag. When taking specimens, the nurse will assist the doctor in pausing flushing, separating the pipeline from the inner cyst of the suction bottle, and pouring the solution out from the entrance of the inner cyst. Two people must cooperate and pour it into a sterile cup. Then, pus is drawn from the sterile cup and injected into the blood oxygen and anaerobic blood culture bottles respectively. After the specimen is taken, the inner cyst is stuffed back into the drainage bottle or directly replaced with a new one. In this process, the operator may be splashed with pus if he is not careful, causing physical and mental discomfort. It also wastes time, human resources, and invisibly increases the cost of patients. In addition, the solution poured out from the inner cyst is kept outside for a long time, and secondary contamination cannot be ruled out.

[0006] In the Chinese patent: 201620258972.5, the name is: A VSD negative pressure suction device capable of instant sampling and detection, which records that: the drainage channel includes at least one double-set pipeline, the double-set pipeline has a drainage section and a negative pressure section; a plurality of drainage holes are opened on the wall of the drainage section, the VSD dressing wraps the drainage section, and the negative pressure section is connected to a negative pressure source;

[0007] At least one sampling tee is provided near the negative pressure section of the double - sleeve pipe. One end of the sampling tee is connected to the drainage section, one end is connected to the negative pressure section, and the other end is connected to a sampling interface; the sampling interface is hermetically connected to a color - developing culture dish and / or a liquid storage bottle.

[0008] As shown in the attached drawings of Patent No. 201620258972.5, this negative - pressure suction device mainly uses a negative - pressure drainage device that can instantaneously sample and detect to instantaneously detect the physiological parameters of wound secretions. This device mainly uses a three - way valve to control the suction of the drainage fluid. When the drainage fluid is sucked into the culture dish, air will enter the culture dish along with it. Therefore, this structure is only suitable for aerobic culture media and is not suitable for anaerobic culture media. Summary of the Invention

[0009] The technical problem to be solved by the present invention is that the existing method for collecting liquid samples of the drainage fluid in the double - sleeve pipe for continuous flushing and negative - pressure suction mentioned in the background technology is inconvenient, requires multiple people to operate, and is prone to exposure and secondary contamination.

[0010] To solve the above - mentioned technical problem, a method for using a double - sleeve pipe for continuous flushing and negative - pressure suction liquid culture medium set is proposed; it is realized through the following technical solutions: A method for using a double - sleeve pipe for continuous flushing and negative - pressure suction liquid culture medium set includes the following steps:

[0011] Step 1: Preparation work: Prepare the culture medium set to be used, confirm and fill in the patient information, paste the patient information on the side walls of the aerobic culture bottle and the anaerobic culture bottle respectively, and prepare a negative - pressure pipe and a negative - pressure gauge for negative - pressure suction.

[0012] Step 2: Collection of drainage fluid in the aerobic culture bottle:

[0013] S21. Take out the aerobic culture bottle from the culture bottle placement box, and then remove the sealing caps on the negative - pressure connector and the liquid inlet connector on the aerobic bottle cap of the aerobic culture bottle.

[0014] S22. Install the negative - pressure pipe connected to the negative - pressure gauge onto the negative - pressure connector, and connect the double - sleeve pipe connected to the patient to the liquid inlet connector.

[0015] S23. Open the negative - pressure gauge, and the negative - pressure gauge provides negative - pressure suction. Suck the drainage fluid in the double - sleeve pipe through the liquid inlet connector into the bottle body of the aerobic culture bottle, and observe the amount of the sucked drainage fluid through the scale on the bottle body. When the amount is sufficient, close the negative - pressure gauge.

[0016] S24. Remove the negative - pressure pipe on the negative - pressure connector and the double - sleeve pipe on the liquid inlet connector, and then cover the caps back onto the negative - pressure connector and the liquid inlet connector.

[0017] S25. After the collection of the drainage fluid in the aerobic culture bottle is completed, place the aerobic culture bottle into one of the culture bottle placement cavities in the culture bottle placement box;

[0018] Step 3: Collection of the drainage fluid in the anaerobic culture bottle:

[0019] S31. Take the anaerobic culture bottle from the culture bottle placement box, and then remove the caps for sealing on the negative pressure connector and the liquid inlet connector on the anaerobic bottle cap of the anaerobic culture bottle;

[0020] S32. Install the negative pressure tube connected to the negative pressure gauge onto the negative pressure connector, and connect the double cannula connected to the patient to the liquid inlet connector;

[0021] S33. Turn on the negative pressure gauge, and the negative pressure gauge provides negative pressure suction. Draw the drainage fluid in the double cannula through the liquid inlet connector into the syringe tube in the injection assembly. The drainage fluid accumulates at the bottom of the syringe tube, and the air drawn into the syringe tube along with the drainage fluid is discharged through the exhaust port until the syringe tube is filled with drainage fluid or the drainage fluid in the syringe tube reaches an appropriate value. Then turn off the negative pressure gauge and stop the negative pressure suction;

[0022] S34. Inject the drainage fluid in the syringe tube into the bottle body of the anaerobic culture bottle. When the syringe tube is filled with drainage fluid and there is no air, proceed to step S35. When there is air in the syringe tube, proceed to step S36;

[0023] S35. Push the syringe plunger in the injection assembly to inject the drainage fluid into the bottle body of the anaerobic culture bottle, and observe the injection volume of the drainage fluid through the scale;

[0024] S36. Push the syringe plunger in the injection assembly to use the air removal assembly to discharge the air in the syringe tube, and then push the syringe plunger to inject the drainage fluid into the bottle body of the anaerobic culture bottle, and observe the injection volume of the drainage fluid through the scale;

[0025] S37. Remove the negative pressure tube on the negative pressure connector and the double cannula on the liquid inlet connector, and then re-cover the caps on the negative pressure connector and the liquid inlet connector;

[0026] S38. After the collection of the drainage fluid in the anaerobic culture bottle is completed, place the anaerobic culture bottle into the other culture bottle placement cavity in the culture bottle placement box;

[0027] Step 4: After the collection of the drainage fluid in the aerobic culture bottle and the anaerobic culture bottle is completed, fill in the collection information on the culture bottle placement box, and send the collected aerobic culture bottle and anaerobic culture bottle for culturing.

[0028] For the optimization of the technical solution of the present invention, in step S33 of step three, the injection tube is communicated with the air removal assembly, and the overflowed drainage fluid in the injection tube is stored in the overflow liquid bin of the air removal assembly. Such a setting facilitates the discharge of air in the injection tube, so that no air enters the anaerobic culture bottle when injecting the drainage fluid into the anaerobic culture bottle, ensuring the accuracy of anaerobic culture.

[0029] For the optimization of the technical solution of the present invention, in steps S35 and S36 of step three, a push rod limit assembly is connected to the injection push rod. The push rod limit assembly restricts the injection of the injection push rod. The setting of the push rod limit assembly facilitates controlling the injection assembly to inject the drainage fluid into the anaerobic culture bottle, which is convenient to use.

[0030] For the optimization of the technical solution of the present invention, the culture medium set includes an aerobic culture bottle, an anaerobic culture bottle, and a culture bottle placement box. The aerobic culture bottle and the anaerobic culture bottle include a bottle body and a bottle cap. The bottle cap is screwed onto the bottle body to seal the bottle body. The aerobic culture bottle for storing the aerobic culture solution and the anaerobic culture bottle for storing the anaerobic culture solution are detachably placed in the culture bottle placement box.

[0031] For the optimization of the technical solution of the present invention, the anaerobic culture bottle includes an air removal assembly and an injection assembly. The injection assembly is connected to the liquid inlet joint on the anaerobic culture bottle, and the air removal assembly is connected to the negative pressure joint on the anaerobic culture bottle. The air removal assembly is communicated with the injection assembly. The air removal assembly discharges the air sucked into the injection assembly, and the injection assembly injects the air-free suction fluid into the bottle body storing the anaerobic bacteria culture solution. The settings of the air removal assembly and the injection assembly facilitate injecting the air-free drainage fluid into the bottle body of the anaerobic culture bottle, preventing air from entering the anaerobic culture bottle and affecting the anaerobic culture of the drainage fluid, ensuring the culture effect.

[0032] For the optimization of the technical solution of the present invention, the bottle body is made of a transparent material, and a scale for observing the quantity of the liquid in the bottle body is provided on the side wall of the bottle body. Such a setting facilitates observing the quantity of the drainage fluid in the culture bottle, which is convenient to use.

[0033] For the optimization of the technical solution of the present invention, the culture bottle placement box includes a partition board and an observation window. The partition board is arranged in the culture bottle placement box, dividing the culture bottle placement box into two culture bottle placement cavities. The observation window is opened on both sides of the culture bottle placement box. The quantity of the liquid in the bottle body is observed through the observation window. The observation window is opened on both sides of the culture bottle placement box, and the quantity of the liquid in the bottle body is observed through the observation window.

[0034] The beneficial effects of the present invention compared with the prior art are:

[0035] The technical solution of the present invention uses a culture bottle placement box to store culture bottles containing two kinds of culture media, aerobic and anaerobic. Two culture bottles are combined and placed in one box. When sampling, they can be directly opened and used respectively, meeting the clinical needs, with simple operation. Also, a negative pressure connector and a liquid inlet connector are directly connected to a negative pressure tube and a double sleeve tube to achieve negative pressure sampling of the drainage fluid. The extraction can be completed at one time, avoiding repeated extraction, with convenient operation and can be operated by a single person. It avoids the contact between medical staff and the drainage fluid, and at the same time, the drainage fluid does not contact the outside world, preventing both occupational exposure and secondary pollution. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 is a three-dimensional schematic diagram of the present invention;

[0037] Figure 2 is a three-dimensional schematic diagram of an aerobic culture bottle;

[0038] Figure 3 is an exploded view of an aerobic culture bottle;

[0039] Figure 4 is a partial cross-sectional view of an aerobic culture bottle;

[0040] Figure 5 is Figure 4 an enlarged view of part A in

[0041] Figure 6 is a three-dimensional schematic diagram of an anaerobic culture bottle;

[0042] Figure 7 is a three-dimensional schematic diagram of an air scavenging component and an injection component;

[0043] Figure 8 is a partial cross-sectional view of an air scavenging component and an injection component;

[0044] Figure 9 is Figure 8 an enlarged view of part B in

[0045] Figure 10 is Figure 8 an enlarged view of part C in

[0046] Figure 11 is a three-dimensional schematic diagram of a push rod limiting component;

[0047] Figure 12 is a partial cross-sectional view of an anaerobic culture bottle;

[0048] Figure 13 is a three-dimensional schematic diagram of a culture bottle placement box;

[0049] Description of reference numerals: 1 - aerobic culture bottle, 11 - bottle body, 12 - aerobic bottle cap, 13 - scale, 14 - hand support, 15 - screw joint, 16 - negative pressure joint, 17 - liquid inlet joint, 18 - gasket, 19 - capping, 2 - elastic sealing assembly, 21 - mounting rod, 22 - sealing cone, 23 - connecting rod, 24 - first spring, 25 - spring mounting block, 3 - anaerobic culture bottle, 31 - anaerobic bottle cap, 32 - functional hole, 4 - air removal assembly, 41 - overflow chamber, 42 - functional chamber, 43 - exhaust pipe, 5 - injection assembly, 501 - injection tube, 502 - injection push rod, 503 - exhaust port sealing rod, 504 - liquid inlet, 505 - injection port, 506 - first exhaust port, 507 - injection piston, 508 - second exhaust port, 509 - sealing head, 510 - second spring, 511 - limit ring, 512 - liquid inlet pipe, 513 - sealing piece, 6 - culture bottle placement box, 61 - partition, 62 - observation window, 63 - culture bottle placement cavity, 7 - push rod limit assembly, 71 - limit rod, 72 - limit groove, 73 - limit card. Detailed implementation mode

[0050] Next, the technical solutions in the embodiments of the present invention will be described in detail with reference to the accompanying drawings in the embodiments of the present invention. Figure 1-13 Embodiment

[0051] As Figure 1 shown, a double - sleeve continuous flushing negative - pressure suction liquid culture medium set includes an aerobic culture bottle 1, an anaerobic culture bottle 3, and a culture bottle placement box 6. The aerobic culture bottle 1 for storing aerobic culture solution is placed in the culture bottle placement box 6, and the anaerobic culture bottle 3 for storing anaerobic culture solution is placed in the culture bottle placement box 6.

[0052] The main function of the aerobic culture bottle 1 is to store aerobic culture solution. At the same time, a negative - pressure joint 16 and a liquid inlet joint 17 are also provided on the aerobic culture bottle 1. The negative - pressure joint 16 is connected to an external negative - pressure tube, and the liquid inlet joint 17 is connected to a double - sleeve on the patient. Through the negative - pressure tube, the drainage liquid in the double - sleeve can be sucked into the aerobic culture bottle 1 through the aerobic culture bottle 1 for culturing the drainage liquid.

[0053] The main function of the anaerobic culture bottle 3 is to store anaerobic culture solution. At the same time, a negative - pressure joint 16 and a liquid inlet joint 17 are also provided on the anaerobic culture bottle 3. The negative - pressure joint 16 is connected to an external negative - pressure tube, and the liquid inlet joint 17 is connected to a double - sleeve on the patient. An air removal assembly 4 and an injection assembly 5 are also provided in the anaerobic culture bottle 3. The air removal assembly 4 and the injection assembly 5 are connected, and the negative - pressure joint 16 and the liquid inlet joint 17 communicate with the air removal assembly 4 and the injection assembly 5.

[0054] The drainage fluid in the double cannula can be sucked into the injection assembly 5 through the negative pressure tube, and the air scavenging assembly 4 can discharge the air carried during the suction of the drainage fluid, so that only the drainage fluid remains in the injection tube 501 of the injection assembly 5, without air. The injection assembly 5 injects the drainage fluid after air scavenging into the anaerobic culture bottle 3 for culturing the drainage fluid.

[0055] The main function of the culture bottle placement box 6 is to serve as a carrier for storing the aerobic culture bottle 1 and the anaerobic culture bottle 3, facilitating the placement of the culture bottles, and also facilitating the medical staff to take and sample the drainage fluid.

[0056] As Figure 1 、 2 As shown in 10 and 3, the aerobic culture bottle 1 includes a bottle body 11, an aerobic bottle cap 12 and an elastic sealing assembly 2. The aerobic bottle cap 12 is detachably screwed onto the bottle body 11, and the elastic sealing assembly 2 is arranged on the aerobic bottle cap 12 to controllably open and close the opening on the aerobic bottle cap 12.

[0057] The bottle body 11 is a hollow rigid plastic bottle with a rectangular cross-section. In this embodiment, it is preferably made of PP plastic. Additionally, for easy observation, the bottle body 11 is transparent. To facilitate the installation of the aerobic bottle cap 12, a circular hole is opened on the upper end surface of the bottle body 11, and a thread is provided on the inner side wall of this circular hole. The through-hole with the thread is named the spiral interface 15, and the aerobic bottle cap 12 is screwed onto the bottle body 11 through the spiral interface 15.

[0058] To facilitate observing the amount of the drained fluid sucked into the bottle body 11 of the aerobic culture bottle 1, a scale 13 is also printed on the outer side wall of the bottle body 11. Through the scale 13, it is convenient to check the amount of the drained fluid sucked into the bottle body 11.

[0059] To facilitate the user to hold the bottle body 11, on one of the sides of the bottle body 11 where the scale 13 is not printed, a handrest 14 is fixedly arranged perpendicular to the side. The handrest 14 is made of flexible plastic, is semi-circular when unfolded, and is sheet-shaped when folded, fitting on the side wall of the bottle body 11. During sampling, the operator reversely buckles the hand into the handrest 14 (like putting on a ring), which can play a fixing role and facilitate the suction operation.

[0060] The aerobic bottle cap 12 is a plastic cap with a rectangular cross-section. On the end surface of the aerobic bottle cap 12, an annular tube protrudes perpendicularly to the end surface. The surface of the annular tube is provided with a thread, and through the thread, the aerobic bottle cap 12 can be screwed onto the spiral interface 15 of the bottle body 11, which is convenient for initially pouring the aerobic culture medium into the bottle body 11 and also for sampling and detecting the culture medium during the later cultivation stage, making it convenient to use.

[0061] To ensure the sealing performance of the connection between the aerobic bottle cap 12 and the bottle body 11, a sealing gasket 18 is placed on the end face of the aerobic bottle cap 12. The sealing gasket 18 is positioned between the aerobic bottle cap 12 and the bottle body 11. When the bottle body 11 and the aerobic bottle cap 12 are tightened, the sealing performance between the two can be improved through the sealing gasket 18.

[0062] To facilitate the entry of the drainage fluid into the aerobic culture bottle 1, a negative pressure connector 16 and a liquid inlet connector 17 are provided on the upper end face of the aerobic bottle cap 12. Both the negative pressure connector 16 and the liquid inlet connector 17 are circular tubular connectors. The negative pressure connector 16 and the liquid inlet connector 17 are fixedly connected to the aerobic bottle cap 12 and communicate with the bottle body 11.

[0063] External threads are provided on the outer side walls of the negative pressure connector 16 and the liquid inlet connector 17. Through the threads, the negative pressure tube connected to the outside world and the negative pressure gauge can be screwed onto the negative pressure connector 16 through a threaded joint. At the same time, the end of the double - tube connected to the patient can be connected to the liquid inlet connector 17 through a threaded joint.

[0064] The negative pressure tube and the negative pressure gauge are existing devices. The main function of the negative pressure gauge is to generate negative pressure. Through the negative pressure gauge, a negative pressure suction force can be generated on the aerobic culture bottle 1, and then the drainage fluid can be attracted into the aerobic culture bottle 1 through the double - catheter and the liquid inlet connector 17, which is convenient to use.

[0065] When not in use, to prevent external dust from falling into the aerobic culture bottle 1 and contaminating the culture solution, caps 19 are also screwed onto the negative pressure connector 16 and the liquid inlet connector 17. The caps 19 are connected to the negative pressure connector 16 or the liquid inlet connector 17 through a traction rope. When in use, unscrew the caps 19, and after use, screw the caps 19 back on to prevent external dust and debris from falling into the aerobic culture bottle 1.

[0066] As Figure 3 、 4 and shown in 5, to prevent the culture solution in the bottle body 11 from leaking from the negative pressure connector 16 and the liquid inlet connector 17 due to shaking during transportation, and at the same time to prevent dust from falling into the negative pressure connector 16 and the liquid inlet connector 17 when the caps 19 are opened and the negative pressure tube or the double - tube is not connected. Also, to further restrict the opening and closing of the negative pressure connector 16 and the liquid inlet connector 17 conveniently, an elastic sealing assembly 2 is provided inside the negative pressure connector 16 and the liquid inlet connector 17. The elastic sealing assembly 2 controls the opening and closing of the negative pressure connector 16 and the liquid inlet connector 17.

[0067] The elastic sealing assembly 2 includes a mounting rod 21, a sealing cone 22, and a first spring 24. The mounting rod 21 is a plastic rod with a rectangular cross - section. The mounting rod 21 is fixed on the inner wall of the negative pressure connector 16 or the liquid inlet connector 17. A circular through - hole penetrating the mounting rod 21 is perpendicularly opened in the middle of the mounting rod 21. The sealing cone 22 can be inserted into the circular through - hole on the mounting rod 21.

[0068] The sealing cone 22 is a truncated cone made of rubber. The diameter of the larger end of the sealing cone 22 is slightly larger than the inner diameters of the negative pressure connector 16 and the liquid inlet connector 17. The diameter of the smaller end of the sealing cone 22 is smaller than the outer diameters of the negative pressure connector 16 and the liquid inlet connector 17. A cylindrical connecting rod 23 is fixed on the smaller end face of the sealing cone 22. The connecting rod 23 can be inserted into the circular through hole on the mounting rod 21, and the connecting rod 23 can move up and down along this circular through hole.

[0069] A circular spring mounting block 25 is fixed at the other end of the connecting rod 23. The diameter of the spring mounting block 25 is smaller than the inner diameters of the negative pressure connector 16 and the liquid inlet connector 17. The first spring 24 is sleeved on the connecting rod 23. One end of the first spring 24 abuts against the spring mounting block 25, and the other end abuts against the mounting rod 21. When the sealing cone 22 is pulled, the connecting rod 23 moves along the circular through hole on the mounting rod 21. At this time, the first spring 24 is compressed under force. After releasing the sealing cone 22, the sealing cone 22 resets under the action of the first spring 24.

[0070] The elastic sealing assembly 2 is provided in both the negative pressure connector 16 and the liquid inlet connector 17. The elastic sealing assembly 2 provided in the negative pressure connector 16 is located at the upper end of the negative pressure connector 16. The sealing cone 22 in the elastic sealing assembly 2 here is stuck on the outer end face of the negative pressure connector 16.

[0071] The elastic sealing assembly 2 provided in the liquid inlet connector 17 is located at the lower end of the liquid inlet connector 17. The sealing cone 22 in the elastic sealing assembly 2 here is stuck on the inner end face of the liquid inlet connector 17.

[0072] Regarding the closing of the negative pressure connector 16 and the liquid inlet connector 17 by the elastic sealing assembly 2, when not in use, the sealing cone 22 is stuck at the port of the negative pressure connector 16 or the liquid inlet connector 17 under the action of the first spring 24. When negative pressure is applied, the sealing cone 22 in the elastic sealing assembly 2 at the negative pressure connector 16 is lifted upward under the action of the negative pressure. At this time, the first spring 24 is compressed under force. When continuous negative pressure is applied, the sealing cone 22 in the elastic sealing assembly 2 at the inner end of the liquid inlet connector 17 moves downward under the action of the negative pressure, opening the liquid inlet connector 17. At this time, the drainage liquid in the double-layer tube can flow into the bottle body 11 through the liquid inlet connector 17. After use, the negative pressure is closed. At this time, under the action of the first spring 24, the sealing cones 22 in the negative pressure connector 16 and the liquid inlet connector 17 reset, re-closing the negative pressure connector 16 and the liquid inlet connector 17.

[0073] Definition: In this embodiment, with the bottle body 11 as the reference, the direction from the bottle body 11 to the bottle cap is the upper direction, the direction from the bottle cap to the bottle body 11 is the lower direction, the direction from the outside to the bottle body 11 is the inner direction, and the direction from the bottle body 11 to the outside is the outer direction.

[0074] Such as Figure 1 、6 As shown in Figures 7, 8, 9, 10 and 12, the anaerobic culture bottle 3 includes a bottle body 11 and an anaerobic bottle cap 31, and the anaerobic bottle cap 31 is detachably screwed to the bottle body 11.

[0075] The bottle body 11 of the aerobic culture bottle 1 is exactly the same as the bottle body 11 of the anaerobic culture bottle 3, and the anaerobic bottle cap 31 is generally the same as the aerobic bottle cap 12, both including a negative pressure connector 16 and a liquid inlet connector 17. The difference between the anaerobic bottle cap 31 and the aerobic bottle cap 12 is that a functional hole 32 for assisting in installing the injection assembly 5 is provided on the anaerobic bottle cap 31, and at the same time, an air removal assembly 4 for removing air from the drainage fluid is also provided on the anaerobic bottle cap 31.

[0076] The main function of the air removal assembly 4 is to discharge the air in the drainage fluid attracted into the injection tube 501 of the injection assembly 5, and finally fill the injection tube 501 with air-free drainage fluid.

[0077] The main function of the injection assembly 5 is to inject the air-free drainage fluid into the anaerobic culture bottle 3 to facilitate the anaerobic culture of the drainage fluid.

[0078] The air removal assembly 4 includes an overflow chamber 41, a functional chamber 42 and an exhaust pipe 43. The functional chamber 42 is connected to the overflow chamber 41, one end of the exhaust pipe 43 is connected to the functional chamber 42, and the other end is connected to the negative pressure connector 16 on the anaerobic bottle cap 31.

[0079] The overflow chamber 41 is a circular tube made of plastic, with both the upper and lower ends of the overflow chamber 41 closed. The main function of the overflow chamber 41 is to store the drainage fluid overflowing from the injection tube 501.

[0080] In order to facilitate connection with the negative pressure connector 16 and thus achieve negative pressure suction, an arc-shaped chamber protrudes from the upper surface of the functional chamber 42, and this chamber is named the functional chamber 42. The lower end of the functional chamber 42 communicates with the overflow chamber 41. A circular connecting pipe is provided on the upper end surface of the functional chamber 42, and this connecting pipe is named the exhaust pipe 43. The exhaust pipe 43 covers the lower end of the negative pressure connector 16 on the anaerobic bottle cap 31, and a connection seal is made at the covering position. In this way, when the negative pressure pipe is connected to the negative pressure connector 16, the overflow chamber 41 will also be in a negative pressure state.

[0081] In order to facilitate the installation of the injection assembly 5, a circular through hole is provided on the upper end surface of the overflow chamber 41, and the injection tube 501 in the injection assembly 5 is inserted into this circular through hole, and a seal is made at the connection between the injection tube 501 and the overflow chamber 41.

[0082] The injection assembly 5 includes an injection tube 501, an injection push rod 502, and an exhaust port sealing rod 503. One end of the injection tube 501 is fixed on the anaerobic bottle cap 31 and covers the function hole 32, and the other end is inserted into the overflow chamber 41. And to ensure the sealing performance and prevent liquid leakage and air leakage at the contact points, the injection tube 501 is sealed at the connection points. The exhaust port sealing rod 503 is arranged in parallel with the injection push rod 502. The exhaust port sealing rod 503 passes through the push plate on the injection push rod 502 and is distributed in parallel with the injection push rod 502. The injection push rod 502 and the exhaust port sealing rod 503 pass through the anaerobic bottle cap 31 and are inserted into the injection tube 501.

[0083] The injection tube 501 is a cylindrical tube, similar in shape to a syringe. The upper end of the injection tube 501 is open, and the injection push rod 502 and the exhaust port sealing rod 503 are inserted into the injection tube 501 through this opening. There is an injection port 505 protruding outward at the lower end of the injection tube 501. The injection port 505 passes through the bottom surface of the overflow chamber 41 and communicates with the bottle body 11 of the anaerobic culture bottle 3. Through the injection port 505, air-free drainage fluid can be injected into the bottle body 11 of the anaerobic culture bottle 3.

[0084] The injection push rod 502 is similar to the push rod of a common syringe, including an injection piston 507 and a push plate. The injection piston 507 passes through the function hole 32 and is inserted into the injection tube 501. Through the injection piston 507, the drainage fluid in the injection tube 501 can be injected into the bottle body 11 of the anaerobic culture bottle 3.

[0085] To form a negative pressure suction path, a circular through-hole penetrating the injection piston 507 is opened on the surface of the injection piston 507, and this through-hole is named the second exhaust port 508. At the same time, on the side wall of the injection tube 501 near the upper end of the injection tube 501 and the function chamber 42, a circular hole penetrating the single side wall of the injection tube 501 is opened, and this circular hole is named the first exhaust port 506. At the corresponding position of the function chamber 42, a circular through-hole is also opened. Through this circular through-hole, the first exhaust port 506, and the second exhaust port 508, the overflow chamber 41 and the injection tube 501 can be communicated.

[0086] On the side wall of the injection tube 501 near the upper end, a circular hole penetrating the single side wall of the injection tube 501 is opened, and this circular hole is named the liquid inlet 504. The height of the liquid inlet 504 is slightly lower than the height of the first exhaust port 506. An "L"-shaped pipe is connected to the liquid inlet 504, and this pipe is named the liquid inlet pipe 512. One end of the liquid inlet pipe 512 is connected to the liquid inlet 504, and the other end covers the lower end surface of the anaerobic bottle cap 31 and covers the lower port of the liquid inlet joint 17. And to ensure that the sealing cone 22 in the elastic sealing assembly 2 in the liquid inlet joint 17 can be opened smoothly, the inner diameter of the liquid inlet pipe 512 is larger than the outer diameter of the sealing cone 22.

[0087] A passage is formed through the liquid inlet pipe 512, the liquid inlet 504, the second exhaust port 508, the first exhaust port 506 and the exhaust pipe 43. When negative pressure suction is applied, the drainage fluid in the double cannula can be sucked into the syringe 501 through the liquid inlet connector 17 and the liquid inlet pipe 512, and at the same time, the air sucked in together with the drainage fluid is discharged through the first exhaust port 506 and the second exhaust port 508.

[0088] When the syringe 501 is filled with drainage fluid and no air is contained, in order to prevent the drainage fluid from entering the overflow chamber 41 through the first exhaust port 506 and the second exhaust port 508 during the injection process, the second exhaust port 508 can be sealed by using the exhaust port sealing rod 503.

[0089] The exhaust port sealing rod 503 includes a sealing head 509. The sealing head 509 is made of rubber material, and the size of the sealing head 509 is slightly larger than the size of the second exhaust port 508. The sealing head 509 is fixed at one end of the exhaust port sealing rod 503. By pressing the exhaust port sealing rod 503, the sealing head 509 can be inserted into the second exhaust port 508 to seal the second exhaust port 508.

[0090] In order to make the sealing of the second exhaust port 508 controllable, an annular limiting ring 511 is fixed on the rod body of the exhaust port sealing rod 503. At the same time, a spring is sleeved outside the exhaust port sealing rod 503, named the second spring 510. One end of the second spring 510 abuts against the anaerobic bottle cap 31, and the other end abuts against the limiting ring 511. When the exhaust port sealing rod 503 does not seal the second exhaust port 508, the exhaust port sealing rod 503 is suspended under the action of the second spring 510, and the opening of the second exhaust port 508 is the initial state. In the initial state, the injection piston 507 is located between the liquid inlet 504 and the first exhaust port 506.

[0091] During negative pressure suction, in order to prevent the gas in the bottle body 11 of the anaerobic culture bottle 3 from being sucked away, a sealing piece 513 is hinged on the overflow chamber 41 through a hinge platform. One end of the sealing piece 513 is hinged on the hinge platform, and the other end covers the injection port 505 to seal the injection port 505. In order to make the sealing piece 513 also seal the injection port 505 under normal conditions, a torsion spring is sleeved on the hinge rod of the sealing piece 513. A force is applied to the sealing piece 513 through the torsion spring, so that the sealing piece 513 can seal the injection port 505 under normal conditions. When injecting the liquid in the syringe 501, the liquid in the syringe 501 squeezes the sealing piece 513, causing the sealing piece 513 to open along the hinge axis. When the injection stops, the sealing piece 513 closes the injection port 505 under the action of the torsion spring.

[0092] Such as Figure 6 、 11As shown in FIGS. 11 and 12, in order to make the injection of the injection push rod 502 controllable, a push rod limit assembly 7 is provided on the anaerobic bottle cap 31. The push rod limit assembly 7 includes a limit rod 71 and a limit card 73.

[0093] The limit rod 71 is a rectangular rod made of plastic. One end of the limit rod 71 is fixed on the upper surface of the anaerobic bottle cap 31, and the other end passes through the push plate on the injection push rod 502. The injection push rod 502 can move up and down along the limit rod 71.

[0094] On one side surface of the limit rod 71, a plurality of rectangular grooves are recessed perpendicular to the limit rod 71. This rectangular groove is named the limit groove 72, and a plurality of limit grooves 72 are arranged at intervals on the limit rod 71.

[0095] The limit card 73 is a card with a cross-section in the shape of "Z". The limit card 73 is hinged on the push plate in the injection push rod 502 through a hinge platform. One end of the limit card 73 can be stuck in the limit groove 72 to limit the injection push rod 502. In order to make the limit of the injection push rod 502 by the limit card 73 controllable, a torsion spring is sleeved on the hinge shaft of the limit card 73. Under the action of the torsion spring, one end of the limit card 73 is always stuck in the limit groove 72. Only by manually pressing the other end of the limit card 73 can the clamping relationship between the limit card 73 and the limit groove 72 be released, thereby realizing the controllable control of the injection of the injection push rod 502.

[0096] Regarding the process of sucking the drainage fluid into the anaerobic culture bottle 3 under negative pressure: When in use, first unscrew the seal cap 19 on the anaerobic bottle cap 31, then screw the negative pressure tube onto the negative pressure joint 16, connect the double tube to the liquid inlet joint 17, and then open the negative pressure gauge on the negative pressure tube. At this time, under the action of negative pressure, the drainage fluid in the double tube flows into the injection tube 501 through the liquid inlet joint 17 and the liquid inlet tube 512. Then continue to suck under negative pressure until the injection tube 501 is filled with drainage fluid. The overflowing drainage fluid flows into the overflow bin 41 through the first exhaust port 506 and the second exhaust port 508. At this time, the negative pressure suction can be stopped. Then first press the exhaust port sealing rod 503 to use the sealing head 509 on the exhaust port sealing rod 503 to seal the second exhaust port 508. Then press the limit card 73 on the push rod limit assembly 7 to release the restriction on the position of the injection push rod 502. Then push the injection push rod 502, and the drainage fluid in the injection tube 501 squeezes the sealing piece 513 at the injection port 505, and the sealing piece 513 opens, and the drainage fluid in the injection tube 501 can be injected into the bottle body 11 of the anaerobic culture bottle 3. After injecting an appropriate amount, stop pushing the injection push rod 502 and release the limit card 73. At this time, the limit card 73 is stuck in the limit groove 72 in the limit rod 71 to limit the position of the injection push rod 502;

[0097] When the drainage fluid in the double cannula is not enough to fill the syringe barrel 501, before pushing the syringe plunger 502, the second exhaust port 508 is not sealed first, and the cover 19 on the negative pressure connector 16 is opened. At this time, the gas in the syringe barrel 501 will be discharged from the negative pressure connector 16 first (here, the elastic force of the torsion spring in the sealing piece 513 needs to be greater than the elastic force of the first spring 24 in the negative pressure connector 16) until part of the drainage fluid flows into the overflow chamber 41, and then the second exhaust port 508 is sealed for injection, ensuring that the drainage fluid injected into the bottle body 11 of the anaerobic culture bottle 3 does not contain air, and ensuring the accuracy of anaerobic culture.

[0098] In addition, when the syringe plunger 502 injects into the bottle body 11, in order to facilitate the smooth entry of the drainage fluid in the syringe barrel 501 into the bottle body 11, a one-way valve can be installed on the bottle body 11 of the anaerobic culture bottle 3. During injection, part of the gas in the bottle body 11 (this gas is pre-filled inert gas, which is an existing technology) is discharged through the one-way valve. At this time, the drainage fluid in the syringe barrel 501 can be smoothly injected into the bottle body 11.

[0099] To facilitate observing the amount of the drainage fluid, both the syringe barrel 501 and the overflow chamber 41 are preferably made of transparent materials.

[0100] As Figure 13 shown, the culture bottle placement box 6 is a box with a rectangular cross-section, and the upper end of the culture bottle placement box 6 is open. The aerobic culture bottle 1 and the anaerobic culture bottle 3 can be placed in the culture bottle placement box 6.

[0101] In the middle of the culture bottle placement box 6, a rectangular partition 61 protrudes vertically from the bottom surface of the culture bottle placement box 6. The culture bottle placement box 6 is divided into two culture bottle placement cavities 63 of the same size by the partition 61. The aerobic culture bottle 1 and the anaerobic culture bottle 3 are respectively placed in the two culture bottle placement cavities 63.

[0102] To facilitate observing the aerobic culture bottle 1 and the anaerobic culture bottle 3 placed in the culture bottle placement cavity 63, a rectangular observation window 62 is respectively opened on the two side surfaces of the culture bottle placement box 6. Through the observation window 62, the aerobic culture bottle 1 and the anaerobic culture bottle 3 placed in the culture bottle placement cavity 63 can be observed.

[0103] The usage method of the double cannula continuous flushing negative pressure suction liquid culture medium set specifically includes the following steps:

[0104] Step 1: Preparation work: Prepare the culture medium set to be used, confirm and fill in the patient information, and paste the patient information on the side walls of the aerobic culture bottle 1 and the anaerobic culture bottle 3 respectively, paying attention not to cover the scale 13. Then prepare the negative pressure tube and negative pressure gauge for negative pressure suction for the preparation before connection.

[0105] Step 2: Collection of the drainage fluid of the aerobic culture bottle 1:

[0106] S21. Take out the aerobic culture bottle 1 from the culture bottle placement box 6, reverse the hand and put it into the hand support 14, and then unscrew the caps 19 for sealing on the negative pressure connector 16 and the liquid inlet connector 17 on the aerobic bottle cap 12 of the aerobic culture bottle 1.

[0107] S22. Screw the negative pressure tube connected to the negative pressure gauge onto the negative pressure connector 16, and screw the double - tube connected to the patient onto the liquid inlet connector 17.

[0108] S23. Open the negative pressure gauge, which provides negative pressure suction. Draw the drainage fluid in the double - tube through the liquid inlet connector 17 into the bottle body 11 of the aerobic culture bottle 1, and observe the amount of the drawn drainage fluid through the scale on the bottle body 11. When the amount is sufficient, close the negative pressure gauge.

[0109] S24. Remove the negative pressure tube on the negative pressure connector 16 and the double - tube on the liquid inlet connector 17, and then screw the cap 19 back onto the negative pressure connector 16 and the liquid inlet connector 17.

[0110] S25. After the drainage fluid collection of the aerobic culture bottle 1 is completed, put the aerobic culture bottle 1 into one of the culture bottle placement cavities 63 in the culture bottle placement box 6.

[0111] Step Three: Collection of drainage fluid from the anaerobic culture bottle 3:

[0112] S31. Take out the anaerobic culture bottle 3 from the culture bottle placement box 6, reverse the hand and put it into the hand support 14, and then unscrew the caps 19 for sealing on the negative pressure connector 16 and the liquid inlet connector 17 on the anaerobic bottle cap 31 of the anaerobic culture bottle 3.

[0113] S32. Install the negative pressure tube connected to the negative pressure gauge onto the negative pressure connector 16, and connect the double - tube connected to the patient to the liquid inlet connector 17.

[0114] S33. Open the negative pressure gauge, which provides negative pressure suction. Draw the drainage fluid in the double - tube through the liquid inlet connector 17 into the syringe tube 501 in the injection assembly 5. The drainage fluid accumulates at the bottom of the syringe tube 501, and the air in the syringe tube 501 is discharged through the exhaust port as the drainage fluid is drawn in. Stop the negative pressure suction until the syringe tube 501 is filled with drainage fluid or the drainage fluid in the syringe tube 501 reaches an appropriate value, and then close the negative pressure gauge.

[0115] S34. Inject the drainage fluid in the syringe tube 501 into the bottle body 11 of the anaerobic culture bottle 3. When the syringe tube 501 is filled with drainage fluid and there is no air, go to step S35; when there is air in the syringe tube 501, go to step S36.

[0116] S35. Press the limit card 73 to unlock the injection push rod 502, push the injection push rod 502 in the injection assembly 5 to inject the drainage fluid into the bottle body 11 of the anaerobic culture bottle 3, and observe the injection volume of the drainage fluid through the scale 13.

[0117] S36. Press the limit card 73 to unlock the injection push rod 502, push the injection push rod 502 in the injection assembly 5 to use the air scavenging assembly 4 to discharge the air in the injection tube 501, then push the injection push rod 502 to inject the drainage fluid into the bottle body 11 of the anaerobic culture bottle 3, and observe the injection volume of the drainage fluid through the scale 13.

[0118] S37. Remove the negative pressure tube from the negative pressure connector 16 and the double sleeve tube from the liquid inlet connector 17, and then cover the caps 19 back on the negative pressure connector 16 and the liquid inlet connector 17.

[0119] S38. After the drainage fluid collection of the anaerobic culture bottle 3 is completed, place the anaerobic culture bottle 3 into another culture bottle placement cavity 63 in the culture bottle placement box 6.

[0120] Step Four: After the drainage fluid collection of the aerobic culture bottle 1 and the anaerobic culture bottle 3 is completed, fill in the collection information on the culture bottle placement box 6, and send the collected aerobic culture bottle 1 and anaerobic culture bottle 3 for culturing.

[0121] The above embodiments are only used to illustrate the technical idea of the present invention, and the protection scope of the present invention cannot be limited thereby. Any modification made on the basis of the technical solution according to the technical idea proposed by the present invention falls within the protection scope of the present invention.

Claims

1. A double-cannula continuous flushing negative pressure suction liquid culture medium set, characterized in that: It includes an aerobic culture bottle (1), an anaerobic culture bottle (3) and a culture bottle placement box (6). The aerobic culture bottle (1) storing aerobic culture medium and the anaerobic culture bottle (3) storing anaerobic culture medium can be detachably placed in the culture bottle placement box (6). Both the aerobic culture bottle (1) and the anaerobic culture bottle (3) are provided with a negative pressure connector (16) and a liquid inlet connector (17). The negative pressure connector (16) is connected to an external negative pressure tube, and the liquid inlet connector (17) is connected to a double cannula on the patient's body. The anaerobic culture bottle (3) further includes an air removal component (4) and an injection component (5). The air removal component (4) is communicated with the injection component (5). The negative pressure connector (16) and the liquid inlet connector (17) on the anaerobic culture bottle (3) are respectively communicated with the air removal component (4) and the injection component (5). The anaerobic culture bottle (3) attracts the drainage fluid in the double cannula into the injection component (5) through a negative pressure tube. The air removal component (4) discharges the air carried by the drainage fluid attracted into the injection component (5), and the drainage fluid with air removed is injected into the anaerobic culture bottle (3) through the injection component (5). The air removal component (4) includes an overflow chamber (41), a functional chamber (42) and an exhaust pipe (43). The lower end of the functional chamber (42) is connected to the overflow chamber (41), and one end of the exhaust pipe (43) is connected to the upper end of the functional chamber (42), and the other end is connected to the negative pressure connector (16) on the anaerobic culture bottle (3). The injection component (5) includes an injection tube (501), an injection push rod (502) and an exhaust port sealing rod (503). One end of the injection tube (501) is fixed on the anaerobic culture bottle cap (31) of the anaerobic culture bottle (3), and the other end is inserted into the overflow chamber (41). The exhaust port sealing rod (503) is arranged in parallel with the injection push rod (502). The injection push rod (502) and the exhaust port sealing rod (503) pass through the anaerobic culture bottle cap (31) and are inserted into the injection tube (501). An "L"-shaped liquid inlet tube (512) is arranged at the upper end of the injection tube (501). One end of the liquid inlet tube (512) is connected to the liquid inlet connector (17), and the other end is connected to the injection tube (501). The height of the circular liquid inlet (504) at the connection of the liquid inlet tube (512) and the injection tube (501) is lower than the height of the first exhaust port (506). The lower end of the injection tube (501) protrudes outward with an injection port (505). The injection port (505) passes through the bottom surface of the overflow chamber (41) and is communicated with the bottle body (11) of the anaerobic culture bottle (3). A circular second exhaust port (508) is provided on the injection piston (507) of the injection push rod (502). The exhaust port sealing rod (503) can be used to seal the second exhaust port (508). A circular first exhaust port (506) is opened on the injection tube (501) near the upper end of the injection tube (501) and on the side wall of the function bin (42). A circular through hole is also opened at the corresponding position of the function bin (42). The overflow bin (41) is communicated with the injection tube (501) through the circular through hole, the first exhaust port (506) and the second exhaust port (508). The air and the overflowing drainage liquid in the injection tube (501) enter the overflow bin (41); the liquid inlet tube (512), the liquid inlet port (504), the second exhaust port (508), the first exhaust port (506) and the exhaust pipe (43) form a passage.

2. The double-cannula continuous flushing negative pressure suction liquid culture medium set according to claim 1, characterized in that: An annular limit ring (511) is fixed on the rod body of the exhaust port sealing rod (503). At the same time, a second spring (510) is sleeved outside the exhaust port sealing rod (503). One end of the second spring (510) abuts against the anaerobic bottle cap (31), and the other end abuts against the limit ring (511). When the exhaust port sealing rod (503) does not seal the second exhaust port (508), the exhaust port sealing rod (503) is suspended under the action of the second spring (510).

3. The double-cannula continuous flushing negative pressure suction liquid culture medium set according to claim 1, characterized in that: A push rod limit assembly (7) is connected to the injection push rod (502), and the push rod limit assembly (7) restricts the injection of the injection push rod (502).

4. The double-cannula continuous flushing negative pressure aspiration liquid culture medium set according to claim 3, characterized in that: The push rod limit assembly (7) includes a limit rod (71) and a limit card (73). One end of the limit rod (71) is fixed on the upper surface of the bottle cap in the anaerobic culture bottle (3), and the other end passes through the push plate on the injection push rod (502). The injection push rod (502) can move up and down along the limit rod (71). The limit card (73) is hinged to the push plate in the injection push rod (502) through a hinge platform. A plurality of limit slots (72) are recessed on one side surface of the limit rod (71) perpendicular to the limit rod. The limit card (73) is stuck in the limit slots (72) in the limit rod (71) to limit the injection push rod (502).

5. The double-cannula continuous irrigation negative pressure suction liquid culture medium set according to claim 1, characterized in that: The bottle body (11) is made of a transparent material, and a scale (13) for observing the amount of liquid in the bottle body (11) is provided on the side wall of the bottle body (11).

6. The double-cannula continuous irrigation negative pressure aspiration liquid culture medium set according to claim 1, characterized in that: The culture bottle placement box (6) includes a partition plate (61) and an observation window (62). The partition plate (61) is arranged in the culture bottle placement box (6) and divides the culture bottle placement box (6) into two culture bottle placement cavities (63). The observation windows (62) are opened on both sides of the culture bottle placement box (6), and the amount of liquid in the bottle body (11) is observed through the observation windows (62).

Images