A medicine for invigorating qi, tonifying lungs and relieving asthma, preparation method and application thereof

Abstract

The present application relates to the technical field of traditional Chinese medicine, and in particular to a kind of medicine for invigorating qi, tonifying lung and relieving asthma, preparation method and application. Its preparation method is step S1. grinding honeysuckle, chrysanthemum, ophiopogon japonicus and momordica grosvenori into powder respectively, mixing by mass ratio and sieving to obtain mixed powder, adding cellulase for enzymolysis; step S2. extinguishing the cellulase, then continuing to decoct, filtering to remove the filter residue, and standing at room temperature; step S3. grinding Cordyceps militaris into powder and sieving to obtain Cordyceps militaris powder, adding alkaline protease and papain for enzymolysis, heating up to extinguish alkaline protease and papain, and standing at room temperature; step S4. the liquid of step S2 and step S3 is mixed with deer lung peptide powder to obtain the medicine of the present application, and the components of the medicine in the present application act synergistically to exert the best curative effect of the medicine.

Description

Technical Field

[0001] The present application relates to the technical field of traditional Chinese medicine, and in particular to a medicine for invigorating qi, tonifying lungs and relieving asthma, a preparation method and application thereof. Background Art

[0002] The lungs are the place for gas exchange between the body and the outside world. The human body inhales oxygen from the air and transports it to various tissues and organs throughout the body, while expelling carbon dioxide produced by metabolism in the body to maintain normal life activities of the human body. Traditional Chinese medicine emphasizes that food and medicine are of the same origin to nourish the lungs. Therefore, qi-invigorating, lung-tonifying and asthma-relieving drugs occupy an important position in the field of traditional Chinese medicine. These drugs effectively relieve and treat symptoms such as asthma and cough caused by lung qi deficiency and lung and kidney deficiency through mechanisms such as invigorating lung qi and relieving cough and asthma. Most of these drugs are mainly Chinese patent medicines, such as qi-invigorating, lung-tonifying and asthma-relieving capsules and lung-tonifying pills. They use the compatibility of traditional Chinese medicines to comprehensively regulate human functions and achieve the purpose of treatment. In clinical applications, qi-invigorating, lung-tonifying and asthma-relieving drugs have achieved significant therapeutic effects and are widely used in the treatment of respiratory diseases such as chronic bronchitis, bronchial asthma, and emphysema. These drugs can not only relieve patients' symptoms such as dyspnea, cough, and sputum, but also improve the quality of life of patients and reduce the recurrence of diseases. In addition, with the continuous deepening of traditional Chinese medicine research, the research and development of qi-invigorating, lung-tonifying and asthma-relieving drugs is also constantly improving. Researchers have used modern scientific and technological means to extract and purify the effective ingredients of traditional Chinese medicine, improving the efficacy and safety of the medicine. At the same time, they have also developed a variety of dosage forms and specifications of medicines to meet clinical needs based on the conditions and constitutions of different patients.

[0003] Although the drugs for replenishing qi, tonifying lung and relieving asthma have achieved remarkable curative effects in clinical application, they still have some disadvantages and limitations: most of the drugs for replenishing qi, tonifying lung and relieving asthma are compound preparations of traditional Chinese medicine with complex ingredients. When used in combination with other drugs, there may be incompatibility taboos, which may affect the efficacy of the drugs or cause adverse reactions. Therefore, it is necessary to pay attention to the interactions and incompatibility taboos between drugs during medication.

[0004] In summary, the existing Qi-invigorating, lung-tonifying and asthma-relieving drugs still have some deficiencies in their formulations. Therefore, it is urgent to propose a Qi-invigorating, lung-tonifying and asthma-relieving drug with a more reasonable formulation to solve the problems existing in the current formulations. Summary of the invention

[0005] In view of the above technical problems, the present application proposes a Qi-invigorating, lung-tonifying and asthma-relieving drug, a preparation method and an application thereof. The technical solution of the present application is as follows:

[0006] In a first aspect, the present application provides a medicine for invigorating qi, tonifying lungs and relieving asthma.

[0007] The drug is prepared from raw materials in the following weight ratio: 43-55 parts of honeysuckle, 12-25 parts of chrysanthemum, 12-20 parts of ophiopogon japonicus, 8-15 parts of momordica grosvenori, 2-6 parts of cordyceps militaris and 2-6 parts of deer lung peptide powder.

[0008] Preferably, it is prepared from the following raw materials in the following weight ratio: 45-52 parts of honeysuckle, 15-22 parts of chrysanthemum, 12-18 parts of ophiopogon japonicus, 9-12 parts of momordica grosvenori, 2-4 parts of cordyceps militaris and 2-4 parts of deer lung peptide powder.

[0009] Preferably, it is prepared from the following raw materials in the following weight ratio: 50 parts of honeysuckle, 20 parts of chrysanthemum, 15 parts of ophiopogon japonicus, 10 parts of momordica grosvenori, 2 parts of cordyceps militaris and 3 parts of deer lung peptide powder.

[0010] The honeysuckle is a traditional Chinese herbal medicine for clearing heat and detoxifying, which is cold in nature, sweet in taste, and enters the lung, heart, stomach, and large intestine meridians. It clears heat and detoxifies, cools and disperses wind-heat, and is used for the prevention and treatment of fever caused by febrile diseases, laryngeal paralysis, wind-heat cold, sore throat, mumps, biliary tract infection, acute and chronic inflammation, erysipelas, etc. Honeysuckle contains pharmacologically active ingredients such as chlorogenic acid and luteolin glycosides, has a broad-spectrum antibacterial effect, and has a strong inhibitory effect on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Shigella dysenteriae, Streptococcus, Pneumococcus, etc.

[0011] The chrysanthemum is pungent, sweet, bitter, and slightly yellow. It enters the lung and liver meridians, has the effects of dispersing wind-heat, clearing the liver and improving eyesight. It is mainly used to treat wind-heat exterior syndrome, early onset of febrile disease, red and swollen eyes, dark eyes, and dizziness.

[0012] The Ophiopogon japonicus is sweet and bitter in nature, slightly cold and enters the heart, lungs and stomach; its main effects are nourishing yin and moistening the lungs, clearing the lungs and resolving phlegm; it is mainly used to treat cough, thirst and constipation.

[0013] The monk fruit is sweet in taste and cool in nature, and enters the lung meridian and spleen meridian, and has the effects of clearing the lung and relieving throat, resolving phlegm and relieving cough, and moistening the intestine and relieving constipation.

[0014] The Cordyceps militaris is sweet and flat in nature, and is suitable for the lung and kidney meridians. It has the effects of tonifying the kidney and lung, stopping bleeding and resolving phlegm, and is suitable for treating hemoptysis or blood in sputum, cough, shortness of breath, night sweats and other symptoms caused by deficiency of lung qi and deficiency of both lung and kidney, pulmonary tuberculosis, etc. The main components of Cordyceps militaris include cordycepin, cordycepic acid, adenosine and polysaccharides, etc., which have good regulatory effects on the endocrine system and nervous system of the human body. Cordycepin can inhibit the growth of certain pathogens and has anticancer active substances. Cordycepic acid can change the microcirculation of the human body, and has the effects of lowering blood lipids and relieving cough and removing phlegm. Cordyceps polysaccharides are immunomodulators that can enhance the body's resistance to viruses and parasites.

[0015] The deer lung peptide powder is a bioactive peptide extracted from deer lungs, has a warm nature, has the effect of moistening the lungs, can relieve coughs, and improve respiratory system symptoms.

[0016] In a second aspect, the present application provides a method for preparing a medicine for invigorating qi, tonifying lungs and relieving asthma, comprising the following steps:

[0017] Step S1. Grind honeysuckle, chrysanthemum, ophiopogon japonicus and monk fruit into powder respectively, mix them according to the mass ratio and sieve them to obtain a mixed powder, add water to the mixed powder and stir to swell it, then add cellulase to hydrolyze it to obtain an enzymatic solution a;

[0018] Step S2. Boil the enzymatic hydrolyzate a obtained in step S1 for 10 to 15 minutes to extinguish the cellulase, then continue to boil for 1 to 2 hours, filter to remove the residue, and stand at room temperature to obtain enzymatic hydrolyzate b;

[0019] Step S3. Grind Cordyceps militaris into powder and sieve to obtain Cordyceps militaris powder, add water to the Cordyceps militaris powder and stir to swell, then add alkaline protease to perform a first enzymolysis, then add papain to perform a second enzymolysis, heat up to extinguish the alkaline protease and papain, and stand at room temperature to obtain enzymolysis solution c;

[0020] Step S4. The enzymatic hydrolysate b, the enzymatic hydrolysate c and the deer lung peptide powder are mixed to obtain a mixed solution d, and auxiliary materials are added to the mixed solution c to make any one of granules, capsules or tablets.

[0021] Preferably, water is added to the step S1 and stirred for 10 to 20 minutes, the mass ratio of the mixed powder, water and cellulase is 10 g: (30 to 50) g: (0.3 to 0.5) mL, the enzymatic hydrolysis temperature is 40 to 50° C., the enzymatic hydrolysis pH is 4.5 to 6.5, and the enzymatic hydrolysis time is 1 to 2 hours.

[0022] Preferably, in step S3, water is added and stirred for 10 to 20 minutes, the temperature of the first enzymolysis is 40 to 50°C, the pH of the first enzymolysis is 9 to 12, the time of the first enzymolysis is 1 to 2 hours, the temperature of the second enzymolysis is 60 to 65°C, the pH of the first enzymolysis is 5 to 8, the time of the second enzymolysis is 1 to 2 hours, and the heating temperature is 70 to 80°C.

[0023] Preferably, in step S3, the mass ratio of Cordyceps militaris powder, water, alkaline protease and papain is 10 g: (30-50) g: (0.2-0.4) mL: (0.2-0.4) mL.

[0024] On the third side, the present application provides an application of a Qi-invigorating, lung-tonifying and asthma-relieving drug, which is used for invigorating Qi-invigorating, lung-tonifying and asthma-relieving.

[0025] Beneficial effects of this application:

[0026] The honeysuckle of the present application is used as the main drug. Honeysuckle is cold in nature, sweet in taste, and enters the lung, heart, stomach, and large intestine meridians. It clears away heat and detoxifies, cools and disperses wind-heat, and is used for the prevention and treatment of febrile fever, throat paralysis, wind-heat cold, sore throat, mumps, biliary tract infection, acute and chronic inflammation, erysipelas, etc. Honeysuckle contains pharmacologically active ingredients such as chlorogenic acid and luteolin glycosides, has a broad-spectrum antibacterial effect, and has a strong inhibitory effect on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Shigella dysenteriae, Streptococcus, Pneumococcus, etc., and is the main drug in the prescription.

[0027] The Ophiopogon japonicus and Chrysanthemum in the present application are used as ministerial drugs together. Chrysanthemum assists Honeysuckle in strengthening the effect of clearing heat and detoxifying. At the same time, Chrysanthemum tastes spicy, sweet, bitter, and slightly yellow. It enters the lung and liver meridians and has the effects of dispersing wind-heat, clearing the liver and improving eyesight. Ophiopogon japonicus is sweet and bitter, slightly cold and enters the heart, lungs, and stomach; Ophiopogon japonicus nourishes yin and moistens the lungs, clears the lungs and resolves phlegm, and has an auxiliary therapeutic effect on symptoms such as cough, thirst, and constipation.

[0028] The Momordica grosvenori and Cordyceps militaris of the present application are used as adjuvants, and the Momordica grosvenori is sweet and cool in nature. It enters the lung meridian and spleen meridian, and has the effects of clearing the lungs and relieving throat, resolving phlegm and relieving cough, and moistening the intestines and relieving constipation. Momordica grosvenori clears the lungs and moistens the intestines, clears the throat, and has an auxiliary therapeutic effect on symptoms such as whooping cough, hypertension, and constipation. Cordyceps militaris is sweet and flat in nature and enters the lung and kidney meridians. It has the effects of tonifying the kidney and lung, stopping bleeding and resolving phlegm, and is suitable for treating hemoptysis or blood in sputum, cough, shortness of breath, night sweats and other symptoms caused by deficiency of lung qi and deficiency of both lung and kidney, pulmonary tuberculosis, etc. The main components of Cordyceps militaris include cordycepin, cordycepic acid, adenosine and polysaccharides, etc., which have a good regulating effect on the endocrine system and nervous system of the human body. Cordycepin can inhibit the growth of certain pathogens and has anti-cancer active substances. Cordycepic acid can change the microcirculation of the human body, and has the effects of lowering blood lipids and relieving cough and expectoration. Cordyceps polysaccharides are immunomodulators that can enhance the body's resistance to viruses and parasites.

[0029] The deer lung peptide powder of the present application is used as a medicine, and the bioactive peptide extracted from the deer lung is warm in nature, has the effect of moistening the lungs, can relieve cough, improve the symptoms of the respiratory system, and guide other drugs to reach the lungs directly, thereby enhancing the overall effect of the prescription. At the same time, the honeysuckle, chrysanthemum, ophiopogon and Momordica grosvenori of the present application are treated with cellulase, and the cellulose in the plant cell wall is decomposed, so that the effective ingredients in the cell are more easily extracted, and the extraction rate of the effective ingredients is improved. The Cordyceps militaris of the present application is enzymatically hydrolyzed, and the macromolecular proteins in the Cordyceps militaris can be decomposed into small molecule polypeptides, which are more easily digested and absorbed by the human body, thereby improving its bioavailability, and at the same time, it is helpful to release the active ingredients in the Cordyceps militaris, such as cordycepin, cordycepic acid, etc., so that these ingredients can play a role more effectively. DETAILED DESCRIPTION

[0030] In order to further explain the technical means and effects adopted by the present application to achieve the predetermined invention purpose, the specific implementation method, structure, characteristics and effects of the present application are described in detail below in combination with the preferred embodiments.

[0031] Example 1

[0032] The present application provides a method for preparing a medicine for replenishing qi, tonifying the lungs and relieving asthma, which is as follows:

[0033] Step S1. 50 g of honeysuckle, 20 g of chrysanthemum, 15 g of ophiopogon japonicus, and 10 g of momordica grosvenori were ground into powders respectively, mixed according to the mass ratio, and passed through a 100-mesh sieve to obtain a mixed powder, 300 g of water was added to the mixed powder and stirred for 10 to 20 min to swell, and then 3 mL of cellulase was added for enzymolysis to obtain an enzymolysis solution a, the enzymolysis temperature was 45 ° C, the enzymolysis pH was 5, and the enzymolysis time was 1.5 h;

[0034] Step S2. The enzymatic hydrolysate a obtained in step S1 was boiled for 13 min to extinguish the cellulase, and then the boiling was continued for 1.5 h, the residue was removed by filtration, and the solution was allowed to stand at room temperature to obtain an enzymatic hydrolysate b;

[0035] Step S3. Grind 2 g of Cordyceps militaris into powder and sieve to obtain Cordyceps militaris powder, add 8 g of water to the Cordyceps militaris powder and stir for 150 min to swell, then add 0.05 mL of alkaline protease for the first enzymolysis at 45 ° C, pH 10, and time for 1.5 h, then add 0.07 mL of papain for the second enzymolysis at 62 ° C, pH 7, and time for 1.5 h, heat to 75 ° C to extinguish alkaline protease and papain for 15 min, and stand at room temperature to obtain enzymolysis solution c;

[0036] Step S4. Mix the enzymatic hydrolysate b, the enzymatic hydrolysate c and 3 g of deer lung peptide powder to obtain a mixed solution d.

[0037] Example 2

[0038] The present application provides a method for preparing a medicine for replenishing qi, tonifying the lungs and relieving asthma, which is as follows:

[0039] Step S1. 45 g of honeysuckle, 20 g of chrysanthemum, 20 g of ophiopogon japonicus, and 10 g of momordica grosvenori were ground into powders respectively, mixed according to the mass ratio, and passed through a 100-mesh sieve to obtain a mixed powder, 475 g of water was added to the mixed powder and stirred for 20 min to swell, and then 2.85 mL of cellulase was added for enzymolysis to obtain an enzymolysis solution a, the enzymolysis temperature was 50 ° C, the enzymolysis pH was 4.5, and the enzymolysis time was 2 h;

[0040] Step S2. The enzymatic hydrolysate a obtained in step S1 is boiled for 15 min to extinguish the cellulase, and then the boiling is continued for 1 h, the residue is removed by filtration, and the solution is allowed to stand at room temperature to obtain an enzymatic hydrolysate b;

[0041] Step S3. Grind 3 g of Cordyceps militaris into powder and sieve to obtain Cordyceps militaris powder, add 15 g of water to the Cordyceps militaris powder and stir for 10 min to swell, then add 0.10 mL of alkaline protease for the first enzymolysis at 40 ° C, pH 9, and time for 1 h, then add 0.12 mL of papain for the second enzymolysis at 60 ° C, pH 5, and time for 2 h, heat to 70 ° C to extinguish the alkaline protease and papain, and stand at room temperature to obtain enzymolysis solution c;

[0042] Step S4. The enzymatic hydrolysate b, the enzymatic hydrolysate c and 2 g of deer lung peptide powder are mixed to obtain a mixed solution d.

[0043] Example 3

[0044] The present application provides a method for preparing a medicine for replenishing qi, tonifying the lungs and relieving asthma, which is as follows:

[0045] Step S1. 50 g of honeysuckle, 15 g of chrysanthemum, 15 g of ophiopogon japonicus, and 15 g of momordica grosvenori were ground into powders respectively, mixed according to the mass ratio, and passed through a 100-mesh sieve to obtain a mixed powder, 285 g of water was added to the mixed powder and stirred for 10 min to swell, and then 4.75 mL of cellulase was added for enzymolysis to obtain an enzymolysis solution a, the enzymolysis temperature was 40 ° C, the enzymolysis pH was 6.5, and the enzymolysis time was 1 h;

[0046] Step S2. Boil the enzymatic hydrolyzate a obtained in step S1 for 10-15 minutes to extinguish the cellulase, then continue boiling for 2 hours, filter to remove the residue, and stand at room temperature to obtain enzymatic hydrolyzate b;

[0047] Step S3. Grind 2 g of Cordyceps militaris into powder and sieve to obtain Cordyceps militaris powder, add 10 g of water to the Cordyceps militaris powder and stir for 20 min to swell, then add 0.08 mL of alkaline protease for the first enzymolysis at a temperature of 40 to 50 ° C, a pH value of 9 to 12, and a time of 1 to 2 h, then add 0.04 mL of papain for the second enzymolysis at a temperature of 65 ° C, a pH value of 8, and a time of 1 h, heat to 80 ° C to extinguish the alkaline protease and papain, and stand at room temperature to obtain an enzymolysis solution;

[0048] Step S4. Mix the enzymatic hydrolysate b, the enzymatic hydrolysate c and 3 g of deer lung peptide powder to obtain a mixed solution d.

[0049] Example 4

[0050] The present application provides a method for preparing a medicine for replenishing qi, tonifying the lungs and relieving asthma, which is as follows:

[0051] Step S1. 43 g of honeysuckle, 25 g of chrysanthemum, 16 g of ophiopogon japonicus, and 8 g of momordica grosvenori were ground into powders respectively, mixed according to the mass ratio, and passed through a 100-mesh sieve to obtain a mixed powder, 400 g of water was added to the mixed powder and stirred for 13 min to swell, and then 4 mL of cellulase was added for enzymolysis to obtain an enzymolysis solution a, the enzymolysis temperature was 40 to 50 ° C, the enzymolysis pH was 4.5 to 6.5, and the enzymolysis time was 1 to 2 h;

[0052] Step S2. The enzymatic hydrolyzate a obtained in step S1 was boiled for 13 min to extinguish the cellulase, and then continued to boil for 1.6 h, filtered to remove the residue, and allowed to stand at room temperature to obtain enzymatic hydrolyzate b;

[0053] Step S3. Grind 4 g of Cordyceps militaris into powder and sieve to obtain Cordyceps militaris powder, add 1 g of water to the Cordyceps militaris powder and stir for 15 min to swell, then add 1 mL of alkaline protease for the first enzymolysis at a temperature of 50 ° C, a pH value of 11, and a time of 1.4 h, then add 0.16 mL of papain for the second enzymolysis at a temperature of 63 ° C, a pH value of 6, and a time of 1.8 h, heat to 75 ° C to extinguish the alkaline protease and papain, and stand at room temperature to obtain enzymolysis solution c;

[0054] Step S4. The enzymatic hydrolysate b, the enzymatic hydrolysate c and 4 g of deer lung peptide powder are mixed to obtain a mixed solution d.

[0055] Example 5

[0056] The present application provides a method for preparing a medicine for replenishing qi, tonifying the lungs and relieving asthma, which is as follows:

[0057] Step S1. 40 g of honeysuckle, 18 g of chrysanthemum, 19 g of ophiopogon japonicus, and 12 g of momordica grosvenori were ground into powders respectively, mixed according to the mass ratio, and passed through a 100-mesh sieve to obtain a mixed powder, 400 g of water was added to the mixed powder and stirred for 15 min to swell, and then 3 mL of cellulase was added for enzymolysis to obtain an enzymolysis solution a, the enzymolysis temperature was 46 ° C, the enzymolysis pH was 6, and the enzymolysis time was 1.5 h;

[0058] Step S2. Boil the enzymatic hydrolyzate a obtained in step S1 for 12 minutes to extinguish the cellulase, then continue to boil for 1.5 hours, filter to remove the residue, and stand at room temperature to obtain enzymatic hydrolyzate b;

[0059] Step S3. Grind 5 g of Cordyceps militaris into powder and sieve to obtain Cordyceps militaris powder, add 10 g of water to the Cordyceps militaris powder and stir for 10 to 20 min to swell, then add 0.15 mL of alkaline protease for the first enzymolysis at 45 ° C, pH 11, and time for 1.5 h, then add 0.2 mL of papain for the second enzymolysis at 60 ° C, pH 7, and time for 1.5 h, heat to 75 ° C to extinguish the alkaline protease and papain, and stand at room temperature to obtain enzymolysis solution c;

[0060] Step S4. The enzymatic hydrolysate b, the enzymatic hydrolysate c and 6 g of deer lung peptide powder are mixed to obtain a mixed solution d.

[0061] Example 6

[0062] The present application provides a method for preparing a medicine for replenishing qi, tonifying the lungs and relieving asthma, which is as follows:

[0063] Step S1. 55 g of honeysuckle, 12 g of chrysanthemum, 12 g of ophiopogon japonicus, and 12 g of momordica grosvenori were ground into powders respectively, mixed according to the mass ratio, and passed through a 100-mesh sieve to obtain a mixed powder, 400 g of water was added to the mixed powder and stirred for 15 min to swell, and then 4 mL of cellulase was added for enzymolysis to obtain an enzymolysis solution a, the enzymolysis temperature was 45 ° C, the enzymolysis pH was 5, and the enzymolysis time was 1.5 h;

[0064] Step S2. The enzymatic hydrolysate a obtained in step S1 is boiled for 10 min to extinguish the cellulase, and then the boiling is continued for 1.5 h, the residue is removed by filtration, and the solution is allowed to stand at room temperature to obtain an enzymatic hydrolysate b;

[0065] Step S3. Grind 3 g of Cordyceps militaris into powder and sieve to obtain Cordyceps militaris powder, add 15 g of water to the Cordyceps militaris powder and stir for 15 min to swell, then add 0.1 mL of alkaline protease for the first enzymolysis at 45 ° C, pH 10, and time 1.5 h, then add 0.1 mL of papain for the second enzymolysis at 65 ° C, pH 8, and time 1.5 h, heat to 75 ° C to extinguish the alkaline protease and papain, and stand at room temperature to obtain enzymolysis solution c;

[0066] Step S4. The enzymatic hydrolysate b, the enzymatic hydrolysate c and 6 g of deer lung peptide powder are mixed to obtain a mixed solution d.

[0067] Comparative Example 1

[0068] The difference between this comparative example and Example 1 is that the added amount of honeysuckle is 20g.

[0069] Comparative Example 2

[0070] The difference between this comparative example and Example 1 is that the added amount of honeysuckle is 70g.

[0071] Comparative Example 3

[0072] The difference between this comparative example and Example 1 is that no deer peptide powder is added.

[0073] Comparative Example 4

[0074] The difference between this comparative example and Example 1 is that the Cordyceps militaris has not been enzymatically hydrolyzed.

[0075] Comparative Example 5

[0076] The difference between this comparative example and Example 1 is that no chrysanthemum is added.

[0077] Comparative Example 6

[0078] The difference between this comparative example and Example 1 is that no Ophiopogon japonicus was added.

[0079] Mouse test example

[0080] The contents and procedures related to animal experiments in this experiment comply with the relevant laws and regulations on the use and management of experimental animals and the relevant regulations of the Institutional Animal Care and Use Committee (IACUC) of this center. 50 male mice were purchased with an average weight of 155g. They were raised normally in the laboratory environment for one week to adapt to the laboratory environment. 45 healthy male mice were selected and randomly divided into 9 groups. Each group was numbered. The first group was numbered F11, F12, F13, F14 and F15; the second group was numbered F21, F22, F23, F24 and F25; the third group was numbered F31, F32, F33, F34 and F35; the fourth group was numbered F41, F42, F43 , F44 and F45; the fifth group is numbered F51, F52, F53, F54 and F55; the sixth group is numbered F61, F62, F63, F64 and F65; the seventh group is numbered F71, F72, F73, F74 and F75; the eighth group is numbered F81, F82, F83, F84 and F85; the ninth group is numbered F91, F92, F93, F94 and F95. The cough-inducing method of ammonia water was used to observe the cough-relieving and asthma-relieving effects of the drug prepared by the present application. Ammonia water is a chemical irritant. After its aerosol is inhaled into the respiratory tract, it stimulates the receptors under the respiratory epithelium and can cause coughing.

[0081] The first group was gavaged with 0.15 ml / 10 g of normal saline, the second group was gavaged with 0.15 ml / 10 g of the mixed solution d prepared in Example 1, the third group was gavaged with 0.15 ml / 10 g of the mixed solution d prepared in Example 4, the fourth group was gavaged with 0.15 ml / 10 g of the mixed solution d prepared in Comparative Example 1, the fifth group was gavaged with 0.15 ml / 10 g of the mixed solution d prepared in Comparative Example 2, the sixth group was gavaged with 0.15 ml / 10 g of the mixed solution d prepared in Comparative Example 3, the seventh group was gavaged with 0.15 ml / 10 g of the mixed solution d prepared in Comparative Example 4, the eighth group was gavaged with 0.15 ml / 10 g of the mixed solution d prepared in Comparative Example 5, and the ninth group was gavaged with 0.15 ml / 10 g of the mixed solution d prepared in Comparative Example 6. The mice were placed in a 500 mL wide-mouth bottle and a cotton ball soaked in concentrated ammonia was placed in it. The coughing movements of mice were observed (abdominal muscle contraction, wide mouth opening, and sometimes coughing sounds), the latency period of coughing of mice was recorded (the time from the placement of cotton balls to the onset of coughing was the latency period), and the antitussive rate was calculated.

[0082] Antitussive rate = (cough latency of medication group - cough latency of normal saline control group) / cough latency of normal saline control group x 100%.

[0083] The test results are shown in Table 1.

[0084] Table 1

[0085]

[0086]

[0087] Case test example

[0088] The test cases are all from 47 male cases with a smoking age of more than 10 years from Nanjing Hongqiang Yupei Diet Therapy Technology Co., Ltd., and exclude patients with standard pulmonary tuberculosis, acute and chronic pharyngitis, various pneumonias and coughs caused by allergies; patients with severe primary diseases such as heart, brain, lung, kidney, liver and hematopoietic system; patients with coughs caused by malignant tumors; patients with severe complications; patients with body temperature>38.0℃; pregnant or lactating women. Take the mixed solution d prepared in Example 1 of this application according to the dosage, the daily dosage is 100ml, taken once in the morning and evening, and tracked for three consecutive weeks. The statistics are shown in Table 2.

[0089] Table 2

[0090] time cough Improved breathing Improved chest tightness Improvement of dry throat and itchy throat Week 1 25 22 28 30 Week 2 10 9 7 5 Week 3 4 5 9 10 Efficiency 83％ 77％ 94％ 96％

[0091] Through the above-mentioned mouse test examples and clinical trial examples, it can be seen that the prepared medicine of the present application has a good effect of relieving cough and relieving asthma, and the various components of the medicine of the present application work synergistically to exert the best therapeutic effect of the medicine. Honeysuckle: As the main medicine, honeysuckle has a significant effect on fever, throat paralysis, wind-heat cold and other symptoms of febrile diseases with its effect of clearing heat and detoxifying, and is the main medicine in the prescription. Chrysanthemum: As a ministerial medicine, chrysanthemum assists honeysuckle to strengthen the effect of clearing heat and detoxifying, and at the same time has the effect of evacuating wind-heat, clearing the liver and improving eyesight. Ophiopogon: Also as a ministerial medicine, Ophiopogon nourishes yin and moistens the lungs, clears the lungs and resolves phlegm, and has an auxiliary therapeutic effect on symptoms such as cough, thirst, and constipation. Momordica grosvenori: As an adjuvant, Momordica grosvenori clears the lungs and moistens the intestines, clears the throat, and has an auxiliary therapeutic effect on symptoms such as whooping cough, hypertension, and constipation. Cordyceps militaris: As an adjuvant, Cordyceps militaris nourishes the kidneys and lungs, stops bleeding and resolves phlegm, and has an auxiliary therapeutic effect on symptoms such as lung qi deficiency, lung and kidney deficiency, and tuberculosis. Deer lung peptide powder: As a guiding drug, deer lung peptide powder moistens the lungs and relieves cough, improves respiratory symptoms, and at the same time guides other drugs directly to the lungs, enhancing the overall effect of the prescription.

[0092] The above is only a preferred embodiment of the present application and does not constitute any form of limitation to the present application. Although the present application has been disclosed as a preferred embodiment as above, it is not intended to limit the present application. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present application. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present application without departing from the content of the technical solution of the present application are still within the scope of the technical solution of the present application.

Claims

1. A medicine for invigorating qi, tonifying the lungs and relieving asthma, characterized in that: The drug is prepared from the following raw materials in weight ratio: 43-55 parts of honeysuckle, 12-25 parts of chrysanthemum, 12-20 parts of ophiopogon japonicus, 8-15 parts of momordica grosvenori, 2-6 parts of cordyceps militaris and 2-6 parts of deer lung peptide powder; The preparation method of the medicine for replenishing qi, tonifying lungs and relieving asthma comprises the following steps: Step S1. Grind honeysuckle, chrysanthemum, ophiopogon japonicus and monk fruit into powder respectively, mix them according to the mass ratio and sieve them to obtain a mixed powder, add water to the mixed powder and stir to swell it, then add cellulase to hydrolyze it to obtain an enzymatic solution a; Step S2. Boil the enzymatic hydrolyzate a obtained in step S1 for 10-15 minutes to extinguish the cellulase, then continue to boil for 1-2 hours, filter to remove the residue, and stand at room temperature to obtain enzymatic hydrolyzate b; Step S3. Grind Cordyceps militaris into powder and sieve to obtain Cordyceps militaris powder, add water to the Cordyceps militaris powder and stir to swell, then add alkaline protease to perform a first enzymolysis, then add papain to perform a second enzymolysis, heat up to extinguish the alkaline protease and papain, and stand at room temperature to obtain enzymolysis solution c; In step S3, water is added and stirred for 10 to 20 minutes, the temperature of the first enzymolysis is 40 to 50 ° C, the pH of the first enzymolysis is 9 to 12, the time of the first enzymolysis is 1 to 2 hours, the temperature of the second enzymolysis is 60 to 65 ° C, the pH of the first enzymolysis is 5 to 8, the time of the second enzymolysis is 1 to 2 hours, and the temperature of the heating is 70 to 80 ° C; Step S4. The enzymatic hydrolysate b, the enzymatic hydrolysate c and the deer lung peptide powder are mixed to obtain a mixed solution d, and auxiliary ingredients are added to the mixed solution c to make any one of granules, capsules or tablets.

2. A Qi-invigorating, lung-tonifying and asthma-relieving drug according to claim 1, characterized in that: The drug is prepared from raw materials in the following weight ratio: 45-52 parts of honeysuckle, 15-22 parts of chrysanthemum, 12-18 parts of ophiopogon japonicus, 9-12 parts of momordica grosvenori, 2-4 parts of cordyceps militaris and 2-4 parts of deer lung peptide powder.

3. A Qi-invigorating, lung-tonifying and asthma-relieving drug according to claim 1, characterized in that: The drug is prepared from raw materials in the following weight ratio: 50 parts of honeysuckle, 20 parts of chrysanthemum, 15 parts of ophiopogon japonicus, 10 parts of momordica grosvenori, 2 parts of cordyceps militaris and 3 parts of deer lung peptide powder.

4. The medicine for invigorating qi, tonifying lungs and relieving asthma according to claim 1, characterized in that: In the step S1, water is added and stirred for 10-20 minutes. The mass ratio of the mixed powder, water and cellulase is 10 g: (30-50) g: (0.3-0.5) mL. The enzymolysis temperature is 40-50° C., the enzymolysis pH is 4.5-6.5, and the enzymolysis time is 1-2 hours.

5. The medicine for invigorating qi, tonifying lungs and relieving asthma according to claim 1, characterized in that: In the step S3, the mass ratio of Cordyceps militaris powder, water, alkaline protease and papain is 10 g: (30-50) g: (0.2-0.4) mL: (0.2-0.4) mL.

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