Cell sorting device and method based on biological culture chip

Abstract

The present application relates to a cell sorting device and cell sorting method based on a biological culture chip. The cell sorting device includes a main body; a loading mechanism including a first loading member for loading the biological culture chip; a chip fixing assembly, which can be placed on the loading mechanism and includes a chip holding member for holding the biological culture chip; an imaging mechanism for acquiring image information of cells to be sorted in the biological culture chip; a display mechanism for displaying the image information of cells to be sorted acquired by the imaging mechanism; and a picking mechanism for extracting cells that meet preset requirements from the biological culture chip. The biological culture chip is first placed in the chip holding member, and then the chip holding member is placed on the loading mechanism, so that the cell sorting device can use the biological culture chip to sort cells, thereby improving the sorting efficiency and accuracy of cell sorting.

Description

Technical Field

[0001] The present application relates to the field of cell sorting, and in particular to a cell sorting device and a cell sorting method based on a biological culture chip. Background Art

[0002] With the development of the biomedical field, cell sorting technology has emerged. It can separate and process a specific cell subpopulation from a mixed cell sample according to the characteristics of the cells, so as to achieve functional analysis of specific cells, especially the sorting and extraction of single cells.

[0003] Bio-culture chips make full use of microscopy or nanotechnology to achieve precise control of cell capture, fixation, and culture on the chip. Through miniaturized chemical analysis methods, they can achieve high-throughput, multi-parameter, in-situ signal detection of cell samples and physical and chemical analysis of cell components, and can realize the fixation and culture of single cells.

[0004] Related technologies include single-cell sorting methods such as mouth pipette technology, limiting dilution technology, and fluorescence flow cytometry. However, single-cell sorting on bio-culture chips using mouth pipette technology and limiting dilution methods suffers from low sorting throughput, high labor intensity, and low sorting efficiency. Furthermore, fluorescence flow cytometry is difficult to integrate with bio-culture chips. Summary of the Invention

[0005] Based on this, it is necessary to provide a cell sorting device and cell sorting method based on a biological culture chip to address the above technical problems, so as to perform cell sorting through the biological culture chip and improve the efficiency and accuracy of cell sorting.

[0006] On the one hand, the present application provides a cell sorting device based on a biological culture chip. The biological culture chip is formed with a holding cavity for accommodating cells to be sorted, and the holding cavities are provided in a plurality and are discretely distributed. The cell sorting device includes: a main body; a loading mechanism, the loading mechanism being configured to be mounted on the main body, the loading mechanism including a first loading member for carrying the biological culture chip, the first loading member moving relative to the main body so that the biological culture chip moves relative to the main body to a preset area; a chip fixing assembly, the chip fixing assembly being capable of being placed on the loading mechanism, the chip fixing assembly including a chip holding member for accommodating the biological culture chip; an imaging mechanism for acquiring image information of cells to be sorted in the biological culture chip, the imaging mechanism being mounted on the main body, the imaging mechanism being configured to image the cells to be sorted in a preset area; a display mechanism for displaying image information of the cells to be sorted acquired by the imaging mechanism; and a selection mechanism for aspirating cells that meet preset requirements in the biological culture chip, the selection mechanism being configured to be mounted on the main body, the selection mechanism including a suction and release member that moves relative to the main body, the suction and release member being configured to aspirate and release cells that meet preset requirements.

[0007] Because the material of the biological culture chip is relatively soft or brittle, the biological culture chip is first placed in a chip container, and then the chip container is placed on a loading mechanism, so that the cell sorting device uses the biological culture chip to sort cells, thereby improving the sorting efficiency and accuracy of cell sorting.

[0008] In one embodiment, the loading mechanism includes a loading assembly and a loading and moving assembly. The loading assembly includes a first loading member for loading the chip holder. The first loading member is mounted on the loading and moving assembly. The loading and moving assembly is capable of driving the loading assembly to move the biological culture chip between the sorting mechanism and the imaging mechanism. By driving the loading and moving assembly, cells to be sorted in different predetermined areas of the cell to be sorted can be observed and aspirated, thereby improving cell sorting efficiency.

[0009] In one embodiment, the mounting mechanism further includes a mounting stop assembly disposed on the mounting assembly, which secures the chip holder to the mounting assembly. The positioning of the mounting stop assembly secures the chip holder to the mounting assembly, thereby enabling the biological culture chip to move with the mounting assembly, thereby enabling the biological culture chip to be used in a cell sorting device.

[0010] In one embodiment, the carrier assembly includes a storage unit positioned on a carrier moving assembly. The storage unit is used to recover cells that meet preset requirements. The imaging mechanism includes an objective lens configured to image the cells to be sorted in a preset area. The carrier moving assembly is capable of driving the storage unit to move so that the storage unit is positioned between the suction and placement member and the objective lens. The arrangement of the storage unit enables the recovery and cultivation of cells that meet preset requirements.

[0011] In one embodiment, the imaging mechanism includes an imaging lighting unit, an optical path component, a detection component, and a fluorescence component. The imaging lighting unit is connected to the selection mechanism and is used to provide illumination for the biological culture chip and the optical path component. The optical path component is used to image the cells to be sorted and transmit image information of the cells to be sorted to the detection component. The fluorescence component includes an excitation light source capable of causing the cells to be sorted to emit fluorescence. The detection component is connected to the display mechanism and is capable of obtaining brightfield image information and fluorescence image information of the cells to be sorted obtained by the optical path component and transmitting the obtained brightfield image information and fluorescence image information of the cells to be sorted to the display mechanism. The imaging mechanism can obtain brightfield image information and fluorescence image information of the cells to be sorted, thereby finding cells that meet preset requirements.

[0012] In one embodiment, the selection mechanism further includes a mounting assembly mounted on the main body, a selection movement assembly, and a recovery assembly. The mounting assembly is used to mount the suction and placement member. The selection movement assembly can drive the suction and placement member to move toward or away from the loading mechanism. The recovery assembly is connected to the suction and placement member and can cause the suction and placement member to aspirate cells that meet preset requirements. The selection mechanism is configured to aspirate cells that meet the preset requirements, thereby achieving cell sorting.

[0013] In one embodiment, the picking mechanism further includes a picking illumination unit connected to the mounting assembly and located at an end of the pick-and-place member away from the loading platform. Light emitted by the picking illumination unit can pass through the pick-and-place member. The provision of the picking illumination unit facilitates calibration of the pick-and-place member, enabling the pick-and-place member to more accurately pick up cells that meet preset requirements.

[0014] In one embodiment, the chip fixing assembly includes a pressure member capable of applying pressure to the biological culture chip to secure the biological culture chip to the chip holder. The pressure member secures the biological culture chip to the chip holder, ensuring that the biological culture chip can move with the chip holder, thereby enabling the biological culture chip to be used in a cell sorting device.

[0015] In one embodiment, the pressure member includes a pressure portion and a retrieval portion. The pressure portion is housed in the chip holder and is capable of applying pressure to the biological culture chip. The retrieval portion is configured to extend circumferentially along an end of the pressure member distal from the biological culture chip and protrude from the chip holder. The retrieval portion facilitates retrieval of the pressure member to facilitate recovery of the biological culture chip.

[0016] Another aspect of the present application provides a cell sorting method, comprising: placing cells to be sorted within a bio-culture chip; placing the bio-culture chip on a loading mechanism; acquiring image information of the cells to be sorted; selecting cells that meet preset requirements based on the image information of the cells to be sorted; and extracting the cells that meet the preset requirements from the bio-culture chip. This cell sorting method utilizes the bio-culture chip for cell sorting, which is beneficial for improving cell sorting efficiency and enabling single-cell sorting. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 Schematic diagram of a cell sorting device in some embodiments of the present application.

[0018] Figure 2 This is an axonometric view of the cell sorting device in some embodiments of the present application after removing the housing assembly.

[0019] Figure 3 for Figure 2 Front view of the cell sorting apparatus with the mechanism removed to show the mechanism.

[0020] Figure 4 for Figure 3 An enlarged view of the selection mechanism in .

[0021] Figure 5 for Figure 4 Axonometric drawing of the selection mechanism in .

[0022] Figure 6 for Figure 4 Right view of the selection mechanism in.

[0023] Figure 7 for Figure 2 Schematic diagram of the suction and placement parts.

[0024] Figure 8 This is an axonometric view of the mounting assembly and lighting assembly of the cell sorting device in some embodiments of the present application.

[0025] Figure 9 This is a front full cross-sectional view of the mounting assembly and the lighting assembly of the cell sorting device in some embodiments of the present application.

[0026] Figure 10 This is a top, full-section view of a mounting assembly and a lighting assembly in some embodiments of the present application.

[0027] Figure 11 This is an axonometric view of the clamping unit of the suction and placement component in some embodiments of the present application.

[0028] Figure 12 This is a cross-sectional view of a clamping unit of a suction and placement component in some embodiments of the present application.

[0029] Figure 13 for Figure 11 Axonometric view of the first clamping drive element of the clamping unit in FIG.

[0030] Figure 14 for Figure 11 Axonometric view of the clamping body of the clamping unit in FIG.

[0031] Figure 15 for Figure 11 A cross-sectional view of the clamping body of the clamping unit in FIG.

[0032] Figure 16 for Figure 11 Axonometric view of the second clamping drive element of the clamping unit in FIG.

[0033] Figure 17 for Figure 8 A top view of a receiving piece for receiving a clamping unit.

[0034] Figure 18 for Figure 8 A sectional view of a receiving piece for receiving a clamping unit.

[0035] Figure 19 This is an axonometric view of the loading mechanism in some embodiments of the present application.

[0036] Figure 20 for Figure 19 Front view of the loading mechanism in.

[0037] Figure 21 for Figure 19 Axonometric view of the loading assembly in the loading mechanism.

[0038] Figure 22 for Figure 19 A front view of the first carrier in the carrier mechanism.

[0039] Figure 23 for Figure 19 Axonometric view of the second loading member and the first loading moving unit in the loading mechanism.

[0040] Figure 24 for Figure 19 Axonometric view of the third loading member and the second loading moving unit in the loading mechanism.

[0041] Figure 25 This is an axonometric view of a storage unit of a cell sorting device in some embodiments of the present application.

[0042] Figure 26 for Figure 25 Axonometric view of the reaction tube in the storage unit.

[0043] Figure 27This is an axonometric diagram of the imaging mechanism of the cell sorting device in some embodiments of the present application.

[0044] Figure 28 Schematic diagram of the imaging mechanism of the cell sorting device in some embodiments of the present application.

[0045] Figure 29 This is a schematic diagram of the control mechanism of the cell sorting device in some embodiments of the present application.

[0046] Figure 30 Schematic diagram of the biological culture chip in some embodiments of the present application.

[0047] Figure 31 Schematic diagram of the receiving chamber of the biological culture chip in some embodiments of the present application.

[0048] Figure 32 Schematic diagram of a receiving cavity of a biological culture chip in some embodiments of the present application, wherein the opening diameter of the receiving cavity is smaller than the bottom diameter of the receiving cavity.

[0049] Figure 33 Schematic diagram of a chip fixing component of a cell sorting device in some embodiments of the present application.

[0050] Figure 34 Schematic diagram of a pressure member in some embodiments of the present application.

[0051] Figure 35 This is a cross-sectional view of the clamping unit in some embodiments of the present application, wherein the spacer, the second clamping drive component and the clamping body are integrally formed.

[0052] Figure 36 This is a top cross-sectional view of a mounting assembly in some embodiments of the present application, wherein the fixing unit includes a fixing member.

[0053] Figure 37 This is a schematic diagram of the operation of the projection mapping of the suction and placement component and the projection marking of the suction and placement component projection identification part in some embodiments of the present application. DETAILED DESCRIPTION

[0054] To make the above-mentioned objects, features, and advantages of the present application more clearly understood, the specific embodiments of the present application are described in detail below with reference to the accompanying drawings. The following description sets forth many specific details to facilitate a full understanding of the present application. However, the present application can be implemented in many other ways than those described herein, and those skilled in the art can make similar improvements without violating the scope of the present application. Therefore, the present application is not limited to the specific embodiments disclosed below.

[0055] In the description of this application, it should be understood that if the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. appear, the orientation or position relationship indicated by these terms is based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing this application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on this application.

[0056] In addition, if the terms "first" or "second" appear, these terms are used for descriptive purposes only and should not be understood to indicate or imply relative importance or implicitly specify the number of technical features indicated. Therefore, a feature specified as "first" or "second" may explicitly or implicitly include at least one of such features. In the description of this application, if the term "plurality" appears, "plurality" means at least two, for example, two, three, etc., unless otherwise specifically defined.

[0057] In this application, unless otherwise specified or limited, the terms "mounted," "connected," "connected," "fixed," etc., should be interpreted broadly. For example, these terms may refer to fixed connections, removable connections, or integration; mechanical connections or electrical connections; direct connections or indirect connections through an intermediary; and internal communication between two components or interaction between two components, unless otherwise specified. Those skilled in the art will understand the specific meanings of these terms in this application based on the specific circumstances.

[0058] In this application, unless otherwise expressly specified or limited, if a first feature is described as being "above" or "below" a second feature, or similar descriptions, this may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediate medium. Furthermore, when a first feature is described as being "above," "above," or "above" a second feature, it may mean that the first feature is directly above or diagonally above the second feature, or simply means that the first feature is at a higher level than the second feature. When a first feature is described as being "below," "below," or "below" a second feature, it may mean that the first feature is directly below or diagonally below the second feature, or simply means that the first feature is at a lower level than the second feature.

[0059] It should be noted that if an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be an intermediate element. If an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be an intermediate element. If any, the terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used in this application are for illustrative purposes only and do not represent the only embodiment.

[0060] Cell sorting is a technique that separates cells that meet predetermined requirements from the cells to be sorted based on their characteristics. When functional analysis of cells that meet certain requirements is required, such as analyzing cell culture supernatant to detect cellular molecules or examining cell function through co-culture, obtaining high-purity cells that meet the predetermined requirements is a prerequisite.

[0061] The cell sorting device is used to automatically select cells that meet preset criteria from the cells to be sorted, separating and purifying them. It offers advantages such as multi-species, high-throughput, multiple sorting, short sorting cycles, and high sorting accuracy. Furthermore, compared to traditional manual sorting, sorting cells using the cell sorting device can reduce human interference, ensure cell consistency during the sorting process, accurately obtain morphologically intact, highly active single cells, improve sorting efficiency, and achieve high-throughput single cell sorting.

[0062] The cell sorting device in some embodiments of the present application is introduced in detail below.

[0063] See Figures 1 to 3 , which shows a schematic diagram of a cell sorting device 100 in some embodiments of the present invention. For ease of comparison and description, the definition Figure 3 The direction perpendicular to the paper surface is a first direction S1, the left-right direction is a second direction S2, and the up-down direction is a third direction S3. The first direction S1, the second direction S2 and the third direction S3 are perpendicular to each other.

[0064] See Figure 1 and Figure 2 The main body 10 is the main frame of the cell sorting device 100, which is used to support and accommodate the main components of the cell sorting device 100. The main body 10 includes a shell component 11 and a support component 12. The shell component 11 forms the storage space of the cell sorting device 100, and the support component 12 supports the mechanism set in the storage space. Figure 2 and Figure 3In some embodiments, the cell sorting device 100 includes a main body 10, a selection mechanism 20, a loading mechanism 30, an imaging mechanism 40, a display mechanism 50, and a control mechanism 60. The main body 10 is used to support and accommodate the other mechanisms in the cell sorting device 100, forming a storage space for the selection mechanism 20, the loading mechanism 30, the imaging mechanism 40, and the control mechanism 60. The loading mechanism 30 is used to load the object to be sorted, and the object to be sorted contains cells to be sorted; the imaging mechanism 40 is used to image the cells to be sorted and obtain image information of the cells to be sorted; the display mechanism 50 is used to display the image information of the cells to be sorted obtained by the imaging mechanism 40, and the image information of the cells to be sorted is processed to select cells that meet the preset requirements in the object to be sorted; the selection mechanism 20 is used to absorb and recover cells that meet the preset requirements in the object to be sorted, thereby achieving the absorption and recovery of cells that meet the preset requirements and completing the cell sorting.

[0065] The shell assembly 11 is mainly used to form a storage space for the internal mechanism of the cell sorting device 100, prevent external light, foreign matter, etc. from entering the storage space, provide a sterile environment for the cell sorting process, prevent cell contamination, and ensure cell activity and normal cell growth.

[0066] The housing assembly 11 is generally a hollow structure, housing the selection mechanism 20, the placement mechanism 30, the imaging mechanism 40, and the control mechanism 60. Typically, the housing assembly 11 is constructed of a rigid material, such as sheet metal, glass, or rigid plastic. The specific material and light transmittance of the housing assembly 11 are determined based on the housing's location and desired functionality. For example, a housing providing overall protection may be constructed of sheet metal, while a light-blocking material may be used to prevent light from entering.

[0067] In some embodiments, see Figure 1 The housing assembly 11 includes a first housing 111 and a second housing 112. The first housing 111 is movable relative to the second housing 112 in a second direction S2. By moving the first housing 111, the objects to be sorted can be placed on the loading mechanism 30 and removed from the cell sorting apparatus 100.

[0068] The first housing 111 is formed of a light-transmitting material. During use, the operation of the cell sorting apparatus 100 is observed through the first housing 111. To prevent external light from interfering with the image information of the cells being sorted obtained by the cell sorting apparatus 100 and to simultaneously observe the internal operation of the cell sorting apparatus 100, the first housing 111 is made of glass or plastic with low light transmittance, such as acrylic.

[0069] The second shell 112 is mainly used to protect the internal components of the cell sorting device 100, and is made of an opaque metal plate. In some embodiments, the second shell 112 in contact with the first shell 111 is made of plastic. On the one hand, it prevents the second shell 112 from causing damage to the first shell 111 when the first shell 111 is opened, and on the other hand, it prevents collision damage to the to-be-sorted parts and the user when taking them out. Furthermore, the second shell 112 is also provided with heat dissipation holes for heat dissipation of the cell sorting device 100. The heat dissipation holes dissipate the heat generated during the operation of the cell sorting device 100, thereby preventing the heat from affecting the activity of the cells to be sorted, which is beneficial to the long-term use of the cell sorting device 100 and realizes high-throughput sorting of cells.

[0070] See again Figure 2 and Figure 3 The support assembly 12 is used to support the main components of the cell sorting device 100 and includes a base 121 and a support base 122. The base 121 is used to carry the main components of the cell sorting device 100. The support base 122 is connected to the base 121 along the third direction S3 to connect and support the main components of the cell sorting device 100.

[0071] The base 121 includes a bottom plate 1211 and legs 1212. The legs 1212 are connected to the bottom plate 1211 along the third direction S3. Adjusting the relative position of the legs 1212 relative to the floor 1211 in the third direction S3 adjusts the overall levelness of the cell sorting apparatus 100, allowing the cell sorting apparatus 100 to be used on various tabletops and platforms. Furthermore, the legs 1212 provide shock absorption and anti-slip properties.

[0072] The support base 122 includes a support plate 1221 and ribs 1222. The support plate 1221 is connected to the base plate 1211 along the third direction S3. To maximize the storage space, the support plate 1221 is positioned on one side of the base plate 1211 in the first direction S1. The support plate 1221 provides connection and support for the main components of the cell sorting apparatus 100 in the third direction S3.

[0073] The ribs 1222 provide auxiliary support for the support plate 1221 and are generally in the shape of a right triangle. One right-angled side extends along the third direction S3 and connects to the support plate 1221 along the first direction S1. The other right-angled side extends along the first direction S1 and connects to the base plate 1211 along the third direction S3. In some embodiments, the support base 122 includes a predetermined number of ribs 1222. In one embodiment, two ribs 1222 are disposed on opposite sides of the base plate 1211 in the second direction S2. The provision of the ribs 1222 further stabilizes the overall frame of the cell sorting apparatus 100.

[0074] To facilitate the assembly and replacement of the support plate 1221, the bottom plate 1211, and the ribs 1222, the support plate 1221, the bottom plate 1211, and the ribs 1222 are all detachably connected, such as by fasteners. Generally, detachable connections include, but are not limited to, threaded connections, screw connections, snap-on connections, clamp connections, flange connections, pin-on connections, and socket connections. It should be noted that the connection method between the support plate 1221, the bottom plate 1211, and the ribs 1222 is not limited. For example, the ribs 1222 can be fixedly connected to the bottom plate 1211 and the support plate 1221 by welding.

[0075] See Figures 4 to 6 , shows the selection mechanism in some embodiments of the present application. The selection mechanism 20 includes a suction and release component 21, a mounting assembly 22, a selection movement assembly 23, a selection lighting unit 24, a cleaning assembly 25, and a recovery assembly 26. The selection mechanism 20 is used to select cells that meet preset requirements from the objects to be sorted. The automated setting of the selection mechanism 20 enables automatic cell suction, improves the efficiency of cell sorting, avoids damage to cells caused by manual operation, facilitates high-throughput cell sorting, and further enables single cell sorting.

[0076] Combine Figure 7 , shows the suction and release parts in some embodiments of the present application. The suction and release part 21 is used to absorb and release cells that meet preset requirements. The suction and release part 21 is provided with a hollow pipe 211 to enable cells to enter the suction and release part 21 and allow the absorbed cells to be stored in the suction and release part 21. The size of at least part of the suction and release part 21 gradually decreases along the radial direction of the suction and release part 21 to form a suction and release port 212 at one end of the suction and release part 21, and the size of the suction and release port 212 matches the size of the cells to be absorbed. During absorption, the cells enter the hollow pipe 211 through the suction and release port 212, and during release, the cells leave the suction and release port 212 through the hollow pipe 211. The design of the suction and release port 212 facilitates accurate and effective absorption of cells, thereby improving the sorting efficiency of the cell sorting device 100. The material and size of the suction and release part 21 can be selected according to the size of the cells to be sorted, such as a 1 mm glass capillary tube is selected for the suction and release part 21.

[0077] See Figures 8 to 10 , shows the mounting assembly and lighting assembly of the cell sorting device in some embodiments of the present application. The mounting assembly 22 is used to install and fix the suction and release member 21 to prevent the suction and release member 21 from falling off during use. Accordingly, the mounting assembly 22 can also be used to replace the suction and release member 21. The mounting assembly 22 includes a clamping unit 221, a fixing unit 222, and a receiving member 223.

[0078] See Figure 11 and Figure 12, shows the clamping unit of the suction and release member in some embodiments of the present application. The clamping unit 221 is used to clamp and release the suction and release member 21 and is connected to the suction and release member 21 along the third direction S3. Figure 7 One end of the suction and placement member 21 is used to absorb and release cells to be sorted and is formed with a suction and placement opening 212. The other end of the suction and placement member 21 is clamped or released by a clamping unit 221. The clamping unit 221 clamps and releases the suction and placement member 21, ensuring stable use. The clamping unit 221 releases the suction and placement member 21, allowing it to be replaced. The clamping unit 221 is provided to secure and release the suction and placement member 21 without damaging it.

[0079] Furthermore, the clamping unit 221 includes at least one clamping member that moves between a clamping position and a release position. The clamping member is used to clamp the other end of the suction and release member 21. In the clamping position, the clamping member clamps the other end of the suction and release member 21 to achieve installation and fixation of the suction and release member 21; in the release position, the clamping member releases the other end of the suction and release member 21 to complete the recovery of the suction and release member 21.

[0080] In some embodiments, the clamping unit 221 includes a clamping body 2211 and a first clamping driver 2212. The first clamping driver 2212 is used to drive the clamping member to move between a clamping position and a release position. The clamping body 2211 is used to support the first clamping driver 2212. The first clamping driver 2212 is also provided with an opening for passing through the suction and placement member 21, and the opening diameter of at least part of the first clamping driver 2212 is slightly larger than the outer diameter of the suction and placement member 21, so that the suction and placement member 21 passes through the first clamping driver 2212.

[0081] In some embodiments, the clamping body 2211 and the first clamping driver 2212 are detachably connected to facilitate installation and replacement of the suction and release member 21. Specifically, the clamping body 2211 and the first clamping driver 2212 can be threadedly connected, with the clamping body 2211 having internal threads and the first clamping driver 2212 having external threads. The connection between the internal and external threads enables the connection between the first clamping driver 2212 and the clamping body 2211. The threaded connection is convenient and reliable, and facilitates installation and removal of the first clamping driver 2212.

[0082] In other embodiments, the clamping body 2211 and the first clamping driver 2212 may be in an interference fit. The interference fit improves the stability of the assembly of the clamping body 2211 and the first clamping driver 2212 and facilitates the clamping member to fix the suction and release member 21. The outer diameter of at least a portion of the first clamping driver 2212 is not less than the inner diameter of the clamping body 2211 to achieve an interference fit between the first clamping driver 2212 and the clamping body 2211.

[0083] In some embodiments, at least a portion of the first clamping driver 2212 is capable of elastic deformation. When the first clamping driver 2212 is connected to the clamping body 2211, the first clamping driver 2212 moves from a release position to a clamping position, and the outer wall of the first clamping driver 2212 contacts and squeezes the inner wall of the clamping body 2211. The first clamping driver 2212 elastically deforms, causing the inner wall of the first clamping driver 2212 to contact and squeeze the outer wall of the suction and placement member 21, thereby clamping and fixing the suction and placement member 21 in the clamping unit 221. In this embodiment, the first clamping driver 2212 is configured as a clamping member.

[0084] In other embodiments, at least a portion of the inner wall size of the first clamping driver 2212 can be changed, such as by configuring the inner wall of the first clamping driver 2212 as a cam surface. When the first clamping driver 2212 is connected to the clamping body 2211, the first clamping driver 2212 moves from the release position to the clamping position, and the inner wall size of the first clamping driver 2212 changes, causing the inner wall of the first clamping driver 2212 to contact and squeeze the outer wall of the suction and placement member 21, thereby securing the suction and placement member 21 in the clamping unit 221. In this embodiment, the first clamping driver 2212 is configured as a clamping member.

[0085] In some embodiments, the clamping unit 221 includes a first clamping drive member 2212, a second clamping drive member 2213, and a clamping body 2211. The second clamping drive member 2213 is used to pass through the suction and placement member 21. The inner diameter of at least part of the second clamping drive member 2213 is slightly larger than the outer diameter of the suction and placement member 21 to accommodate the suction and placement member 21. The second clamping drive member 2213 is arranged between the first clamping drive member 2212 and the clamping body 2211 along the third direction S3. The arrangement of the first clamping drive member 2212 and the second clamping drive member 2213 allows at least two parts of the suction and placement member 21 to be accommodated, thereby improving the installation stability of the suction and placement member 21.

[0086] In some embodiments, the second clamping drive member 2213 is fixedly connected to the clamping body 2211, such as the second clamping drive member 2213 is welded to the clamping body 2211 or the second clamping drive member 2213 and the clamping body 2211 are integrally formed. In other embodiments, the second clamping drive member 2213 and the clamping body 2211 can be combined in a manner that is one or more of clearance, interference, elastic, and surface-bonded. The second clamping drive member 2213 and the clamping body 2211 can also be threadedly connected. It is understood that as long as the second clamping drive member 2213 can be accommodated and placed, the connection method between the second clamping drive member 2213 and the clamping body 2211 is not limited.

[0087] In some embodiments, one end of the second clamping driver 2213 in the third direction S3 is accommodated in the first clamping driver 2212, and the inner diameter of at least a portion of the first clamping driver 2212 is slightly larger than the outer diameter of the second clamping driver 2213. The other end of the second clamping driver 2213 in the third direction S3 is accommodated in the clamping body 2211, and the inner diameter of at least a portion of the clamping body 2211 is slightly larger than the outer diameter of the second clamping driver 2213. Accommodating and securing at least two portions of the second clamping driver 2213 facilitates stable installation of the second clamping driver 2213.

[0088] In some embodiments, at least a portion of the second clamping drive member 2213 is capable of elastic deformation. When the second clamping drive member 2213 is connected to the first clamping drive member 2212, the outer wall of the second clamping drive member 2213 contacts and squeezes the inner wall of the first clamping drive member 2212, causing the second clamping drive member 2213 to elastically deform. The inner wall of the second clamping drive member 2213 contacts and squeezes the outer wall of the suction and placement member 21, thereby securing the suction and placement member 21 to the second clamping drive member 2213. In this embodiment, the second clamping drive member is configured as a clamping member.

[0089] In some embodiments, at least a portion of the inner wall size of the second clamping drive member 2213 can be changed, such as the inner wall of the second clamping drive member 2213 is configured as a cam surface. When the second clamping drive member 2213 is connected to the first clamping drive member 2212, the inner wall size of the second clamping drive member 2213 changes, causing the inner wall of the second clamping drive member 2213 to contact and squeeze the outer wall of the suction and placement member 21, thereby achieving the fixation of the suction and placement member 21 in the clamping unit 221. In this embodiment, the second clamping drive member 2213 is configured as a clamping member.

[0090] In some embodiments, the clamping unit 221 includes a first clamping driver 2212, a clamping body 2211, and a first elastic member 2214. The first elastic member 2214 is configured between the first clamping driver 2212 and the clamping body 2211 in the third direction S3. The inner diameter of the first elastic member 2214 in a natural state is slightly larger than the outer diameter of the suction and release member 21. In this embodiment, the first elastic member 2214 is configured as a clamping member. The elastic deformation of the first elastic member 2214 in the radial direction is changed by adjusting the displacement between the first clamping driver 2212 and the clamping body 2211, thereby enabling the first elastic member 2214 to clamp or release the suction and release member 21. Specifically, the first clamping driver 2212 is provided with an external thread, and the clamping body 2211 is provided with an internal thread. The elastic deformation of the first elastic member 2214 in the radial direction is changed by adjusting the tightening or loosening of the external thread and the internal thread, thereby enabling the first elastic member 2214 to clamp or release the suction and release member 21.

[0091] In some embodiments, the clamping unit 221 includes a first clamping driver 2212, a clamping body 2211, a second clamping driver 2213 and a first elastic member 2214. The first elastic member 2214 is configured between the first clamping driver 2212 and the second clamping driver 2213 along the third direction S3. When the clamping unit 221 is installed, the first elastic member 2214 is elastically deformed in the radial direction by adjusting the displacement between the first clamping driver 2212 and the second clamping driver 2213. The first elastic member 2214 is configured as a first clamping member. Specifically, the first clamping driver 2212 and the second clamping driver 2213 co-extrudes the first elastic member 2214 on the opposite end faces in the third direction S3, causing the first elastic member 2214 to deform in the radial direction, thereby clamping and fixing the suction and release member 21.

[0092] In this embodiment, the clamping body 2211 or the second clamping drive 2213 is configured as a first hollow structure for accommodating the suction and release member 21, and the first clamping drive 2212 is configured as a second hollow structure for accommodating the suction and release member 21. The combined action of the first hollow structure, the second hollow structure and the first clamping member 2214 enables the suction and release member 21 to be supported and clamped.

[0093] Furthermore, the clamping unit 221 also includes a spacer 2215 disposed between the second clamping driver 2213 and the first elastic member 2214 in the third direction S3. The spacer 2215 is used to pass through the suction and placement member 21. That is, the inner diameter of the spacer 2215 is slightly larger than the outer diameter of the suction and placement member 21. The provision of the spacer 2215 allows at least three portions of the suction and placement member 21 to be accommodated, allowing the second clamping driver 2213 to be adapted for the installation of different suction and placement members 21.

[0094] In some embodiments, see Figure 35 The spacer 2215, the second clamping driver 2213, and the clamping body 2211 are integrally formed. By adjusting the displacement between the first clamping driver 2212 and the clamping body, the displacement between the spacer 2215 and the first elastic member 2214 is changed, and the elastic deformation of the first elastic member 2214 in the radial direction is changed, thereby achieving the clamping or release of the suction and release member 21 by the first clamping member 2214.

[0095] Furthermore, the clamping unit 221 is provided with a first elastic member 2214 and a second elastic member 2216 spaced apart along the third direction S3. The first elastic member 2214 and the second elastic member 2214 are arranged at both ends of the spacer 2215 along the third direction S3. Specifically, the first elastic member 2214 is arranged to be located between the first clamping driver 2212 and the spacer 2215, and the second elastic member 2216 is arranged to be located between the second clamping driver 2213 and the spacer 2215. The elastic deformation of the first elastic member 2214 in the radial direction is changed by adjusting the displacement between the first clamping driver 2212 and the spacer 2215, and the elastic deformation of the second elastic member 2216 in the radial direction is changed by adjusting the displacement between the second clamping driver 2213 and the spacer 2215, so that the first elastic member 2214 and the second elastic member 2216 can clamp or release the suction and release member 21. In this embodiment, the first elastic member 2214 is configured as a first clamping member, and the second elastic member 2216 is configured as a second clamping member. The arrangement of the first elastic member 2214 and the second elastic member 2216 clamps at least two parts of the suction and placement member 21, ensuring the installation stability of the suction and placement member 21.

[0096] Specifically, the first clamping drive member 2212 is provided with an external thread, and the clamping body 2211 is provided with an internal thread. By adjusting the tightening or loosening of the external thread and the internal thread, the radial elastic deformation of the first clamping member 2214 and the second clamping member 2216 is changed, thereby realizing the clamping or release of the suction and release member by the first clamping member 2214 and the second clamping member 2216.

[0097] The second clamping drive member 2213 is configured as a first hollow structure for accommodating the suction and release member 21, the first clamping drive member 2212 is configured as a second hollow structure for accommodating the suction and release member 21, and the spacer 2215 is configured as a third hollow structure for accommodating the suction and release member 21. The combined action of the first hollow structure, the second hollow structure, the third hollow structure, the first clamping member 2214 and the second clamping member 2216 supports and clamps the suction and release member 21.

[0098] For specific embodiments, see Figure 13, shows the first clamping drive member of the clamping unit in some embodiments of the present application. The first clamping drive member 2212 includes a first clamping drive portion 2212a, an operating portion 2212b and a second clamping drive portion 2212c. The first clamping drive portion 2212a is provided with a through hole for accommodating at least part of the second clamping drive member 2213, the first elastic member 2214, the spacer 2215 and the second elastic member 2216. The first clamping drive portion 2212a is provided with an external thread, and the first clamping drive member 2212 is connected to the internal thread of the clamping body 2211 through the external thread of the first clamping drive portion 2212a. The inner diameter of the first clamping drive portion 2212a is slightly larger than the outer diameter of at least part of the second clamping drive member 2213, and the outer diameter of at least part of the first clamping drive portion 2212a is slightly larger than the inner diameter of at least part of the clamping body 2211. The second clamping driving portion 2212 c is used to accommodate the suction and placement member 21 , and the inner diameter of the second clamping driving portion 2212 c is slightly larger than the outer diameter of the suction and placement member 21 .

[0099] The operating portion 2212b is radially convexly arranged on the first clamping drive portion 2212a and the second clamping drive portion 2212c, and is configured between the first clamping drive portion 2212a and the second clamping drive portion 2212c. The operating portion 2212b inner diameter is slightly larger than the outer diameter of the suction and release member 21. The operating portion 2212b is used to cooperate with external equipment, and realizes the connection of the first clamping drive portion 2212 and the clamping body 2211 through the operating portion 2212b. Specifically, the outer peripheral surface of the operating portion 2212b comprises a relatively arranged circular arc portion and a relatively arranged flat portion, and the flat portion is used to cooperate with external equipment such as a wrench, so that the external thread of the first clamping drive portion 2212 is connected to the internal thread of the clamping body 2211, and realizes the clamping of the suction and release member 21.

[0100] In some embodiments, see Figure 14 and Figure 15, shows the clamping body of the clamping unit in some embodiments of the present application. The clamping body 2211 includes a first clamping body portion 2211a and a second clamping body portion 2211b connected to the first clamping body portion 2211a. The first clamping body portion 2211a is provided to connect the hollow pipe 211 of the suction and placement member 21 with the recovery assembly 26, so as to achieve the cleaning of the suction and placement member 21 and the absorption and release of cells that meet the preset requirements. The first clamping body portion 2211a is provided with a first accommodating cavity 2211c. On the one hand, it is used to accommodate at least part of the second clamping drive member 2213 to achieve communication between the first accommodating cavity 2211c and the hollow pipe 211 of the suction and placement member 21, and on the other hand, it is used to connect with at least part of the selection lighting unit 24 to achieve illumination of the hollow pipe 211 of the suction and placement member 21. Specifically, the first accommodating cavity 2211c includes a joint portion, which is stepped and is used to connect with the selection lighting unit 24 to achieve illumination of the suction and placement member 21. The central axis of the coupling portion coincides with the central axis of the suction and placement member 21 to ensure that the selection lighting unit 24 provides an illuminating effect on the suction and placement member 21. It is understood that the shape of the coupling portion can be adjusted as needed, and is not limited to a stepped shape, as long as it can be connected to the selection lighting unit 24. Furthermore, the first clamping body portion 2211a is provided with a connecting hole in the radial direction, the connecting hole being in communication with the first accommodating cavity 2211c, and the connecting hole being used for connection to the recovery assembly 26.

[0101] The second clamping body portion 2211b is provided with a second accommodating cavity 2211d in communication with the first accommodating cavity 2211c, for connecting the first clamping driver 2212 and accommodating the second clamping driver 2213. The first clamping driver 2212 is configured to at least partially extend into the second accommodating cavity 2211d, so that at least a portion of the first clamping driver 2212 can be accommodated and connected to the clamping body 2211. The first clamping driver 2212 is configured to at least partially extend beyond the second accommodating cavity 2211d to form an operating portion 2212b that facilitates the movement of the first clamping driver 2212. The surface of at least a portion of the second clamping body portion 2211b gradually changes along the third direction S3 to form a tapered surface. This arrangement facilitates the fixing and adjustment of the clamping unit 221. The inner diameter of at least part of the second clamping body 2211b is slightly larger than the outer diameter of the first clamping drive part 2212a. Specifically, the second clamping body 2211b is provided with an internal thread, and the second clamping drive part 2212a is provided with an external thread, and the internal thread is connected to the external thread.

[0102] In some embodiments, see Figure 16, shows the second clamping drive member of the clamping unit in some embodiments of the present application. The second clamping drive member 2213 is a hollow structure, which is used for the penetration of the suction and release member 21 to achieve the installation and accommodation of the suction and release member 21. The second clamping drive member 2213 includes a fourth clamping drive portion 2213a, a second flange portion 2213b and a fifth clamping drive portion 2213c. The inner diameters of the fourth clamping drive portion 2213a, the second flange portion 2213b and the fifth clamping drive portion 2213c are slightly larger than the outer diameter of the suction and release member 21 to achieve the penetration and accommodation of the suction and release member 21. Combined Figure 12 and Figure 15 At least part of the fourth clamping drive part 2213a is accommodated in the first clamping main body part 2211a along the third direction S3 to achieve the combination of the second clamping drive part 2213 and the clamping main body 2211. The outer diameter of the fourth clamping drive part 2213a is slightly smaller than the inner diameter of at least part of the first clamping main body part 2211a. Figure 12 and Figure 13 At least part of the fifth clamping drive portion 2213c is accommodated in the first clamping drive portion 2212a to achieve the combination of the second clamping drive member 2213 and the first clamping drive member 2212. The outer diameter of the fifth clamping drive portion 2213c is slightly smaller than the inner diameter of the first clamping drive portion 2212a.

[0103] The second flange portion 2213b is protruded from the fourth clamping drive portion 2213a and the fifth clamping drive portion 2213c in the third direction S3 and is disposed between the fourth clamping drive portion 2213a and the fifth clamping drive portion 2213c. Figure 12 and Figure 15 , the clamping unit 221 also includes a sealing member 2217 for fixing the second clamping drive member 2213. The sealing member 2217 is configured between the second flange portion 2213b and the first clamping body portion 2211a in the third direction S3. The shape and size of the inner diameter of the sealing member 2217 match the shape and size of the outer diameter of the fourth clamping drive portion 2213a. In this embodiment, the sealing member 2217 is accommodated in the second accommodating chamber 2211d and supported by the second flange portion 2213b. When the clamping unit 221 is installed, the sealing member 2217 prevents the communication between the first accommodating chamber 2211c and the second accommodating chamber 2211d, so that the suction and release member 21 for the cells to be sorted passes through the first accommodating chamber 2211c and directly passes through the suction and release member 21 without passing through the second accommodating chamber 2211d, so as to avoid the second accommodating chamber 2211d affecting the suction and release member 21 for the cells to be sorted. In other embodiments, the sealing member 2217 may also be accommodated in the first accommodating cavity 2211c to prevent the first accommodating cavity 2211c from communicating with the second accommodating cavity 2211d.

[0104] The first elastic member 2214, the second elastic member 2216 and the sealing member 2217 are all annular elastic elements, such as O-rings. It should be noted that the shape, size, number and type of the first elastic member 2214, the second elastic member 2216 and the sealing member 2217 are not limited.

[0105] Furthermore, in order to facilitate the installation and replacement of the suction and release member 21 and avoid damage to the suction and release member 21 during installation, the central axes of the first clamping drive member 2212, the first elastic member 2214, the spacer 2215, the second elastic member 2216 and the second clamping drive member 2213 in the third direction S3 all coincide with the central axis of the suction and release member 21 in the third direction S3.

[0106] When the clamping unit 221 is installed, the first elastic member 2214, the spacer 2215, the second elastic member 2216 and the second clamping drive member 2213 are placed into the first clamping drive member 2212 in sequence along the third direction S3, and one end of the second clamping drive member 2213 away from the second elastic member 2216 is accommodated in the first accommodating cavity 2211c of the first clamping main body 2211a along the third direction S3, and the other end abutting the second elastic member 2216 is accommodated in the accommodating cavity of the first clamping drive member 2212 along the third direction S3.

[0107] When the suction and release member 21 is installed, the suction and release member 21 passes through the through holes formed by the first clamping drive member 2212, the first clamping member 2214, the spacer 2215, the second clamping member 2216 and the second clamping drive member 2213 in sequence along the third direction S3 until the end face of the suction and release member 21 is flush with the end face of the second clamping drive member 2213 accommodated in the clamping body 2211. Then, the first clamping drive member 2212 is tightened to cause the first clamping member 2214 and the second clamping member 2216 to undergo radial elastic deformation to fix the suction and release member 21, and to cause the sealing member 2217 to undergo radial deformation to fix the second clamping drive member 2213 and block the first accommodating cavity 2211c and the second accommodating cavity 2211d. When the suction and placement member 21 is recovered or replaced, the first clamping driving member 2212 is released to restore the first clamping member 2214 and the second clamping member 2216 to their original states, and then the suction and placement member 21 is taken out in a direction opposite to the installation direction.

[0108] In some embodiments, reference Figure 11The first clamping driver 2212 is provided with a clamping mark 2212d. The clamping mark 2212d is provided on the surface of the first clamping driver 2212. The clamping mark 2212d is used to rotate the first clamping driver 2212 to a preset position to clamp the suction and release member 21. The preset position is the clamping position. Because the suction and release member adopts a glass capillary design, the provision of the clamping mark 2212d effectively prevents damage to the glass capillary due to overtightening of the first clamping driver 2212. In other embodiments, the clamping mark 2212d corresponds to a mark on the clamping body 2211 or located on other components. When the two are aligned, it is the clamping position.

[0109] See again Figures 8 to 10 In some embodiments, a fixing unit 222 is provided along the radial direction of the clamping unit 221 for fixing and adjusting the clamping unit 221. The fixing unit 222 includes a first fixing member 2221 and a second fixing member 2222. The first fixing member 2221 and the second fixing member 2222 are spaced apart along the circumference of the clamping unit 221 and extend along the radial direction of the clamping unit 221. The provision of the first fixing member 2221 and the second fixing member 2222 secures at least two parts of the clamping unit 221.

[0110] Combine Figure 12 and Figure 15 The first fixing member 2221 and the second fixing member 2222 are spaced apart along the circumference of the second clamping body portion 2211b of the clamping body 2211. Specifically, when the clamping unit 221 is fixed, one radial end of the first fixing member 2221 in the second clamping body portion 2211b abuts against the tapered surface of the fifth clamping drive portion 2213c, and one radial end of the second fixing member 2222 in the fifth clamping drive portion 2213c also abuts against the tapered surface of the fifth clamping drive portion 2213c.

[0111] Furthermore, the first fixing member 2221 and the second fixing member 2222 are fine-tuning members, both of which are configured as micrometer structures, for fixing the clamping unit 221 and fine-tuning the clamping unit 221 so that the suction and release member 21 can be in a preset position. Typically, the micrometer structure includes a screw, a fixed sleeve, a differential cylinder and a knob. When fixing, tighten the first fixing member 2221 and the second fixing member 2222 to achieve the fixing of the clamping unit 221. When fine-tuning, rotate the knob and differential cylinder of the first fixing member 2221 and the knob and differential cylinder of the second fixing member 2222 until the suction and release member 21 is in a preset position.

[0112] In some embodiments, the fixing unit 222 further includes a third fixing member 2223. The third fixing member 2223 is arranged along the circumference of the clamping unit 221 and extends along the radial direction of the clamping unit 221. The arrangement of the third fixing member 2223 supports and fixes the clamping unit 221 in at least three parts.

[0113] In some embodiments, the fixing unit further includes an elastic biasing element 2224 for biasing the third fixing member 2223. One end of the elastic biasing element 2224 is connected to the third fixing member 2223. The third fixing member 2223 and the elastic biasing element 2224 can be, for example, a combination of a spring seat and a spring. The third fixing member 2223 abuts against the clamping unit 221, overcoming the biasing force of the elastic biasing element 2224 to move the first fixing member 2221 or the second fixing member 2222, thereby adjusting the position of the clamping unit 221. During fixation, the third fixing member 2223 and the elastic biasing element 2224 can provide elastic support for the clamping unit 221, ensuring the installation stability of the clamping unit 221. Furthermore, the third fixing member 2223 is arranged along the first direction S1, and the first fixing member 2221 and the second fixing member 2222 are symmetrically arranged on either side of the third fixing member 2223 in the first direction S1. It can be understood that as long as the suction and release member 21 can be fixed, the number and type of the fixing units 222 can be set according to actual needs and are not limited here.

[0114] See Figures 17 to 18 , Figure 17 and Figure 18 Schematic diagram of the container in some embodiments of the present application is shown. Figures 8 to 10 The accommodating member 223 is a hollow structure for accommodating at least part of the clamping unit 221. A first opening 2231 and a second opening 2232 are respectively provided on the two end surfaces of the accommodating member 223 in the third direction S3. The clamping unit 221 passes through the first opening 2231 and the second opening 2232 in sequence along the third direction S3 to place the clamping unit 221. The clamping unit 221 passes through the second opening 2232 and the first opening 2231 in sequence to remove the clamping unit 221. The size of the first opening 2231 is larger than that of the clamping unit 221. The position of the clamping unit 221 relative to the first opening 2231 can be adjusted by moving the first fixing member 2221, and the position of the clamping unit 221 relative to the first opening 2231 can also be adjusted by moving the second fixing member 2222.

[0115] Combine Figure 15 The size of the first opening 2231 is smaller than the outer diameter of the first clamping body 2211a and larger than the outer diameter of the second clamping body 2211b, that is, at least part of the clamping body 2211 protrudes from the first opening 2231 in the third direction S3. Figure 13The diameter of the second opening 2232 is smaller than the outer diameter of the operating portion 2212b and larger than the outer diameter of the second clamping drive portion 2212c, so as to allow the suction and placement member 21 and the first clamping drive member 2212 to pass through. The clamping unit 221 is installed and accommodated in the accommodating member 223 through the first opening 2231 and the second opening 2232.

[0116] The receiving member 223 is further provided with a first mounting hole 2233, a second mounting hole 2234 and a third mounting hole 2235 for mounting the fixing unit 222. The first mounting hole 2233, the second mounting hole 2234 and the third mounting hole 2235 are all arranged along the radial direction of the clamping unit 221. Figure 10 The first mounting hole 2233, the second mounting hole 2234, and the third mounting hole 2235 are radially arranged along the tapered surface of the second clamping body 2211b. At least a portion of the first fixing member 2221 passes through the first mounting hole 2233 and enters the first opening 2231, abutting against the clamping unit 221. At least a portion of the second fixing member 2222 passes through the second mounting hole 2234 and enters the first opening 2231, abutting against the clamping unit 221. At least a portion of the third fixing member 2223 passes through the third mounting hole 2235 and enters the first opening 2231, abutting against the clamping unit 221. The elastic biasing element 2224 is located in the third mounting hole 2235 and biases the third fixing member 2223 toward the center of the first opening 2231.

[0117] In order to achieve the fixation of the first fixing member 2221 and the second fixing member 2222, the container 223 is further provided with a first fixing hole 2236 and a second fixing hole 2237. The fastener fixes the first fixing member 2221 to the container 223 through the first fixing hole 2236, and fixes the second fixing member 2222 to the container 223 through the second fixing hole 2237. Figure 18 The first fixing holes 2236 and the second fixing holes 2237 are spaced apart along the second direction S2 on the receiving member 223. Fasteners are screwed into the fixing holes and abut against the first fixing member 2221 and the second fixing member 2222, thereby securing the first fixing member 2221 and the second fixing member 2222 to the receiving member 223. It should be noted that the structure of the receiving member 223 can be adjusted according to actual design and is not limited to the above structure.

[0118] When installing the clamping unit 221, loosen the first fixing member 2221 and the second fixing member 2222, remove the clamping unit 221 from the receiving member 223 along the third direction S3, install the suction and release member 21 into the clamping unit 221, and repeat the above process of installing the suction and release member 21 into the clamping unit 221, so that the suction and release member 21 is fixedly installed in the clamping unit 221. Thereafter, the clamping unit 221 equipped with the suction and release member 21 is placed in the receiving member 223 along the third direction S3, and the first fixing member 2221 and the second fixing member 2222 are tightened. It is necessary to overcome the biasing force applied to the clamping unit by the third fixing member 2223 and the elastic biasing element 2224 to achieve the installation of the clamping unit 221.

[0119] It should be noted that the fixing unit 222 only needs to include one of the above fixing members. Figure 36 The fixing unit 222 includes a third fixing member 2223 and an elastic biasing element 2224. One end of the elastic biasing element 2224 is connected to the third fixing member 2223. The third fixing member 2223 abuts against the clamping unit 221, so that the biasing force of the elastic biasing element 2224 is overcome to fix the clamping unit 221 to the accommodating member 223, thereby realizing the installation and fixation of the clamping unit 221.

[0120] See again Figures 4 to 6 In some embodiments, the selection moving component 23 is used to drive the suction and release component 21 to move relative to the main body 10 along the third direction S3 to achieve cell suction and release. The selection moving component 23 includes a selection driving component 231, a selection transmission component 232 and a selection moving component 233. Under the control of the control mechanism 60, the selection driving component 231 drives the selection transmission component 232 to rotate and thereby achieve the movement of the selection moving component 233 in the third direction S3. Figure 8 The accommodating member 223 is connected to the selection movable member 233 along the first direction S1. Under the drive of the selection driving member 231 and the rotation of the selection transmission member 232, the selection movable member 233 drives the suction and placement member 21 to move along the third direction S3. In the specific embodiment, the selection driving member 231 is a stepping motor, which realizes the quantitative movement of the suction and placement member 21 and ensures the movement accuracy of the suction and placement member 21. The displacement accuracy of the suction and placement member 21 in this embodiment is 2 microns. The selection transmission member 232 is a lead screw extending along the third direction S3 to realize the movement of the suction and placement member 21 along the third direction S3.

[0121] The receiving member 223 is detachably connected to the selection and movement member 233 via fasteners. To facilitate installation of the receiving member 223, the selection and movement member 233 is provided with a coupling groove. This coupling groove facilitates the positioning and installation of the receiving member 223 on the selection and movement member 233. It should be understood that the coupling method between the selection and movement member 233 and the installation assembly 22 is not limited, as long as the selection and movement member 23 can drive the installation assembly 22.

[0122] In some embodiments, the picking assembly 23 further includes a picking guide 234 and a picking slide 235. The picking guide 234 extends along the third direction S3. The picking slide 235 is disposed on the picking guide 234 and connected to the picking member 233, enabling movement of the picking member 233 in the third direction S3. The arrangement of the picking guide 234 and the picking slide 235 facilitates movement of the picking member 233. It should be noted that the extension lengths of the picking guide 234 and the picking transmission member 232 are determined by the travel range of the suction member and are not limited herein.

[0123] In some embodiments, the picking movement assembly 23 further includes a picking displacement sensor 236 and a picking stopper 237. The picking displacement sensor 236 is disposed on one side of the picking guide 234 in the second direction S2. The provision of the picking displacement sensor 236 facilitates the acquisition of the movement distance of the suction and placement member 21, thereby assisting the control mechanism 60 in controlling the movement of the suction and placement member 21. It should be noted that the type and installation location of the picking displacement sensor 236 are not limited as long as the transmission of the displacement signal can be achieved.

[0124] The selection limiter 237 extends along the third direction S3 and is connected to the selection movable member 233. The selection limiter 237 is disposed on one side of the selection guide member 234 in the second direction S2. The selection limiter 237 and the selection displacement sensor 236 can be disposed on the same side of the selection guide member 234, or they can be disposed on either side of the selection guide member 234. The selection limiter 237 limits the movement distance of the suction and placement member 21 in the third direction S3. The selection displacement sensor 236 can determine the highest and lowest positions of the suction and placement member 21, thereby preventing the suction and placement member 21 from colliding with the support mechanism 30 during movement and causing damage.

[0125] Under the control of the control mechanism 60, the selection driving member 231 drives the selection moving member 233 to move along the selection guide member 234 in the third direction S3, driving the suction and release member 21 connected to the selection moving member 233 to move in the third direction S3, thereby enabling the suction and release member 21 to approach and move away from the cells to be sorted and enabling the suction and release member 21 to absorb and release the cells to be sorted.

[0126] See again Figure 8 and Figure 9 The selection lighting unit 24 is connected to the mounting assembly 22 and is located at one end of the suction and placement member 21 away from the carrier mechanism 30. The selection lighting unit 24 includes a selection light source 241 connected to the control mechanism 60. The selection light source 241 and the suction and placement member 21 are arranged along the third direction S3. The light emitted by the selection light source 241 can pass through the hollow pipe 211 of the suction and placement member 21 to form a projection of the suction and placement member 21, thereby facilitating the positioning of the suction and placement member 21. Figure 15, the light source 241 is selected and disposed in the first accommodating cavity 2211 c of the clamping body 2211 .

[0127] In order to ensure that the suction and release part 21 can absorb and release cells, a sealing connection is required between the selection light source 241 and the first accommodating chamber 2211c to achieve the blocking of the first accommodating chamber 2211c and the external environment. In one embodiment, the selection light source 241 is fixed in the first accommodating chamber 2211c of the first clamping body portion 2211a by gluing. The gluing connection is easy to operate and can ensure the sealing between the selection light source 241 and the clamping body 2211 so that the suction and release part 21 can achieve the absorption of cells under the drive of the recovery component 26. It is understandable that as long as the fixing of the selection light source 241 is achieved, the connection mode of the selection light source 241 and the clamping body 2211 is not limited.

[0128] In some embodiments, the selected light source 241 is an LED light source, which is fixedly connected to the clamping body 2211. The central axis of the LED light source is parallel to or aligned with the central axis of the suction and placement member 21, so that the light emitted by the LED light source can pass through the hollow tube 211 of the suction and placement member 21. The LED light source has high lighting efficiency, long service life, and is easy to install, ensuring the long-term use of the cell sorting device 100. It should be noted that the position of the selected light source 241 is set according to the position of the suction and placement member 21. As long as it can emit light, the type of the selected light source 241 is not limited to an LED light source.

[0129] In a feasible embodiment, a sealing element is further provided between the selection light source 241 and the first clamping body portion 2211a. The sealing element needs to allow light to pass through the hollow pipe 211 of the suction and placement member 21. The provision of the sealing element provides a buffer for the selection light source 241 and further ensures the sealing between the suction and placement member 21 and the clamping body 2211.

[0130] In some embodiments, combined Figure 37 As shown, the cell sorting device 100 includes a suction and placement member projection recognition unit, which is used to identify the suction and placement member projection and form a suction and placement member projection map. A projection mark is provided within the suction and placement member projection map. The suction and placement member projection can be adjusted by the first fixing member 2221 and the second fixing member 2223, thereby changing the position of the suction and placement member projection map relative to the projection mark, so that the projection mark is located at a preset position of the suction and placement member projection map to complete the calibration of the suction and placement member 21.

[0131] During use, the control mechanism 60 controls the selected light source 241 to emit light, and the light passes through the hollow pipe 211 of the suction and release member 21 and passes through the suction and release member 21 through the suction and release port 212. The suction and release member projection is obtained according to the light passing through the suction and release port 212, and the image information (such as a circular aperture) of the suction and release member projection is obtained through the imaging mechanism 40. Based on the image information and the projection mark (such as a cross cursor) in the image information, the first fixing member 2221 or the second fixing member 2223 is moved to adjust the position of the cross cursor in the circular aperture, such as moving the cross cursor to the middle position of the circular aperture. After marking the position of the suction and release member projection, this position is used as a reference to facilitate the subsequent control mechanism 60 to control the selection moving component 23 to drive the suction and release member 21 to move in the third direction S3, so as to achieve the absorption of cells that meet the preset requirements.

[0132] See again Figure 4 and Figure 5 The cleaning component 25 is used to clean the suction and release part 21 before use, so that the activity of cells can be guaranteed during the cell absorption and release process without damaging the normal state of cells. The cleaning component 25 includes a driving unit 251 and a cleaning liquid unit 254. The driving unit 251 is connected to the hollow pipe 211 of the suction and release part 21 through the connecting piece 252, which can change the air pressure of the hollow pipe 211 of the suction and release part 21, thereby realizing the cleaning liquid entering the hollow pipe 211 of the suction and release part 21, thereby realizing the cleaning of the suction and release part 21. Combined Figure 2 The driving unit 251 is disposed on the support plate 1221 along the first direction S1. To facilitate the absorption of the cleaning liquid, the driving unit 251 is connected to the suction and release member 21 along the third direction S3.

[0133] In some embodiments, the driving unit 251 uses a pump, which makes the diaphragm inside the pump do reciprocating motion through a mechanical device, thereby compressing and stretching the air in the pump chamber to form a negative pressure, so that the suction and discharge port 212 of the suction and discharge component 21 produces a pressure difference with the external atmospheric pressure. Under the action of the pressure difference, the cleaning liquid is sucked into the suction and discharge component 21. Figure 4 、 Figure 12 and Figure 15 The driving unit 251 is connected to the connecting hole of the clamping body 2211 , and the clamping body 2211 and the suction and release member 21 form a cleaning chamber for cleaning the hollow pipe 211 of the suction and release member 21 .

[0134] In some embodiments, see Figure 4 and Figure 5 To facilitate the connection between the drive unit 251 and the clamping body 2211, the cleaning assembly 25 further includes a connecting piece 252. One end of the connecting piece 252 is connected to the drive unit 251, and the other end is connected to the connection hole of the clamping body 2211. This connects to the hollow conduit 211 of the suction and discharge member 21, creating a pressure differential at the suction and discharge port 212. Specifically, the connecting piece 252 can be a plastic hose.

[0135] Furthermore, to facilitate the connection between the clamping body 2211 and the connecting piece 252, the cleaning assembly 25 also includes a passage connector 253. One end of the passage connector 253 is connected to the clamping body 2211 through a connection hole, and the other end allows the connecting piece 252 to be sleeved onto the passage connector 253. The passage connector 253 can be a pagoda connector, with one end threadedly connected to the clamping body 2211 and the other end sleeved with the connecting piece 252, ensuring a good seal between the connecting piece 252 and the connecting piece 252.

[0136] The passage connecting piece 253 is a hollow structure, forms passage together with the communicating piece 252 and the suction and release part 21. During cleaning, cleaning fluid flows through the inwall of the suction and release part 21, the inwall of the clamping body 2211 and the inwall of the passage connecting piece 253, cleans. Be threadedly connected between the passage connecting piece 253 and the clamping body 2211. It will be appreciated that as long as the connection of the passage connecting piece 253 and the clamping body 2211 can be realized, the connection mode of the passage connecting piece 253 and the clamping body 2211 is not limited, as the passage connecting piece 253 and the clamping body 2211 can be integrally formed.

[0137] In some embodiments, to facilitate the placement of the cleaning liquid, the cleaning assembly 25 further includes a holding unit 255 for holding the cleaning liquid unit 254, and the holding unit 255 is located on the loading mechanism 30 of the cell sorting device 100. The cleaning liquid unit 254 includes a first cleaning liquid 2541 and a second cleaning liquid 2542, and the first cleaning liquid 2541 and the second cleaning liquid 2542 are different from each other. The holding unit 255 includes a first holding part 2551 and a second holding part 2552, the first holding part 2551 is used to hold the first cleaning liquid 2541, and the second holding part 2552 is used to hold the second cleaning liquid 2542. The driving unit 251 draws the first cleaning liquid 2541 into the hollow pipe 211 to perform a first preset number of cleanings on the suction and placement member 21 and draws the second cleaning liquid 2542 into the hollow pipe 211 to perform a second preset number of cleanings on the suction and placement member 21.

[0138] Specifically, in this embodiment, the first cleaning liquid 2541 is ethanol, the second cleaning liquid 2542 is phosphate-buffered saline (PBS), and both the first holding member 2551 and the second holding member 2552 are biological culture dishes. Ethanol is used to sterilize and disinfect the suction and placement member 21, and the PBS buffer is used to provide an internal environment suitable for cell culture, thereby ensuring that the interior of the suction and placement member 21 does not affect cell activity. It is understood that the type of cleaning liquid is not limited as long as the suction and placement member 21 can be cleaned. For example, the second cleaning liquid 2542 can also be HEPES (hydroxyethylpiperazine ethanesulfonic acid) buffer.

[0139] Furthermore, the first cleaning solution 2541 uses 75% ethanol, and the second cleaning solution 2542 uses 0.01M PBS buffer. 75% ethanol can effectively eliminate live bacteria and viruses. It should be noted that the first cleaning solution 2541 and the second cleaning solution 2542 can be configured to obtain the desired concentration, or the first cleaning solution 2541 and the second cleaning solution 2542 can be used directly. Accordingly, the concentration of the cleaning solution can be set according to actual needs and is not limited to the above concentration.

[0140] Preferably, in order to ensure a sterile environment during the cell sorting process, the first cleaning fluid 2541 and the second cleaning fluid 2542 need to be filtered before use. Specifically, both ethanol and PBS buffer are filtered through 0.45 microns before use. The filtration size requirement can be set according to the experimental needs and is not limited to 0.45 microns, such as 0.22 micron filtration. In a feasible embodiment, before cleaning, the holding part assembly and the cleaning fluid assembly are sterilized, such as by sterilization in an autoclave. As long as the sterilization and disinfection of the holding part and the cleaning fluid can be achieved, the specific sterilization method is not limited.

[0141] Before the suction and placement member 21 absorbs cells, the driving unit 251 and the suction and placement member 21 are first connected, and then the first holding member 2551 is taken, and an appropriate amount of the first cleaning liquid 2541 is added. The first holding member 2551 with the first cleaning liquid 2541 is spaced apart from the suction and placement member 21 along the third direction S3. Under the control of the control mechanism 60, the selection moving assembly 23 drives the suction and placement port 212 of the suction and placement member 21 to move into the first cleaning liquid 2541. The driving unit 251 drives the suction and placement member 21 to absorb the first cleaning liquid 2541 into the hollow pipe 211 of the suction and placement member 21 to clean the suction and placement member 21. The suction and placement member 21 is driven to release the first cleaning liquid 2541 into the first holding member 2551. The action of absorbing and releasing the first cleaning liquid 2541 can be one or more times, and the specific number of times is determined according to a preset number of times to complete the first cleaning step and disinfect the hollow pipe 211 of the suction and placement member 21.

[0142] Afterwards, take the second holding member 2552, add an appropriate amount of the second cleaning fluid 2542, and place the second holding member 2552 with the second cleaning fluid 2542 and the suction and release member 21 at intervals along the third direction S3. Under the control of the control mechanism 60, select the mobile assembly 23 to drive the suction and release port 21 to move into the second cleaning fluid 2542, the drive unit 251 drives the suction and release member 21 to absorb the second cleaning fluid 2542 into the hollow pipe 211 of the suction and release member 21 to clean the suction and release member 21, drive the suction and release member 21 to release the second cleaning fluid 2542 into the second holding member 2552, and the action of absorbing and releasing the second cleaning fluid 2542 can be 1 or more times, and the specific number of times is determined according to the preset number of times to complete the second step of cleaning. The second step of cleaning cleans the first cleaning fluid 2541 on the one hand, and provides a relatively stable ionic environment and pH buffering capacity to facilitate the survival of cells in the suction and release member 21 on the other hand.

[0143] After the suction and placement member 21 absorbs cells, the specific steps are opposite to those before the suction and placement member absorbs cells. Specifically, first take the second holding member 2552, add an appropriate amount of the second cleaning fluid 2542, and place the second holding member 2552 with the second cleaning fluid 2542 and the suction and placement member 21 at intervals along the third direction S3. Under the control of the control mechanism 60, the selection moving assembly 23 drives the suction and placement port 21 to move into the second cleaning fluid 2542, the driving unit 251 drives the suction and placement member 21 to absorb the second cleaning fluid 2542 into the hollow pipe 211 of the suction and placement member 21 to clean the suction and placement member 21, and drives the suction and placement member 21 to release the second cleaning fluid 2542 into the second holding member 2552. The action of absorbing and releasing the second cleaning fluid 2542 can be one or more times, and the specific number of times is determined according to the preset number of times to complete the first step of cleaning, and the residual cells or cell buffer in the suction and placement member 21 are cleaned.

[0144] Afterwards, the first holding member 2551 is taken, and an appropriate amount of the first cleaning liquid 2541 is added, and the first holding member 2551 with the first cleaning liquid 2541 is spaced apart from the suction and placement member 21 along the third direction S3. Under the control of the control mechanism 60, the moving assembly 23 is selected to drive the suction and placement port 212 of the suction and placement member 21 to move into the first cleaning liquid 2541, and the driving unit 251 drives the suction and placement member 21 to absorb the first cleaning liquid 2541 into the hollow pipe 211 of the suction and placement member 21 to clean the suction and placement member 21, and drives the suction and placement member 21 to release the first cleaning liquid 2541 into the first holding member 2551. The action of absorbing and releasing the first cleaning liquid 2541 can be one or more times, and the specific number of times is determined according to the preset number of times to complete the second step of cleaning, on the one hand, cleaning the second cleaning liquid 2542, and on the other hand, disinfecting and sterilizing the hollow pipe 211 of the suction and placement member 21.

[0145] In some embodiments, the control mechanism 60 includes a cleaning control unit 61, which is used to control the driving unit 251 to draw a first cleaning liquid 2541 into the hollow tube 211 to clean the suction and placement member 21 a first preset number of times, and to control the driving unit 252 to draw a second cleaning liquid 2542 into the hollow tube 211 to clean the suction and placement member 21 a second preset number of times. The cleaning control unit 61 can control the driving unit 251 to allow the drawn first cleaning liquid 2541 to reciprocate along the third direction S3 in the hollow tube 211 for multiple cleanings, and then release it into the first receiving member 2551; and control the driving unit 251 to allow the drawn second cleaning liquid 2542 to reciprocate along the third direction S3 in the hollow tube 211 for multiple cleanings, and then release it into the second receiving member 2552.

[0146] See again Figure 4 and Figure 5 Recovery assembly 26 is used to absorb and release cells that meet preset requirements. Recovery assembly 26 includes a drive unit 251, a connecting member 252, and a passage connector 253. The specific functional and positional relationships are described in conjunction with cleaning assembly 25 and will not be repeated here. Under the control of control mechanism 60, recovery assembly 26 facilitates cell sorting and release, facilitating the cell sorting process and improving cell sorting efficiency.

[0147] When the recovery assembly 26 is in use, the cells that meet the preset requirements are moved to be spaced apart from the suction and placement member 21 along the third direction S3. Under the control of the control mechanism 60, the selection movement assembly 23 drives the suction and placement port 212 of the suction and placement member 21 to move to a preset position. At this time, there is a preset distance between the suction and placement port 212 and the cells that meet the preset requirements. The control mechanism 60 controls the drive unit 251 to generate a pressure difference at the suction and placement port 212 of the suction and placement member 21, thereby sucking the cells that meet the preset requirements into the hollow conduit 211, completing the suction of the cells that meet the preset requirements. The preset distance is the distance that enables the suction and placement member 21 to suck the cells that meet the preset requirements into the hollow conduit 211.

[0148] To facilitate the installation of the selected mobile assembly 23, refer to Figure 4The selection mechanism 20 further includes a mounting bracket 27. The mounting bracket 27 is provided to securely mount the selection moving assembly 23. The selection mechanism 20 is mounted on the main body 10 via the mounting bracket 27. In some embodiments, the mounting bracket 27 is connected to the imaging mechanism 40 along the third direction S3, and the imaging mechanism 40 is mounted on the main body 10. It should be noted that the mounting bracket 27 can be configured as needed. For example, the mounting bracket 27 can be connected to the support plate 1221 along the first direction S1 to secure the selection mechanism 20 on the main body 10. In some embodiments, the mounting bracket 27 is provided with a first mounting seat 271 and a second mounting seat 272. The first mounting seat 271 is provided on the mounting bracket 27 along the third direction S3 and is used to securely mount the selection guide member 234. The second mounting seat 272 is provided on the mounting bracket 27 along the first direction S1, perpendicular to the first mounting seat 271, and is used to position and secure the selection drive member 231.

[0149] When the selection mechanism 20 is in use, the installation component 22 and the selection moving component 23 are connected, the suction and placement component 21 is installed and fixed on the installation component 22, the selection moving component 23 drives the suction and placement component 21 to move, and the suction and placement component 21 is cleaned by the cleaning component 25. Then, the selection moving component 23 drives the suction and placement component 21 to move, and the recovery component 26 makes the suction and placement component 21 absorb and recover cells that meet the preset requirements at a preset position. Finally, after the absorption is completed, the selection moving component 23 drives the suction and placement component 21 to move, and the suction and placement component 21 is cleaned by the cleaning component 25.

[0150] In some embodiments, see Figure 19 and Figure 20 , shows the loading mechanism in some embodiments of the present application. The loading mechanism 30 is used to carry and place the objects to be sorted, and the objects to be sorted contain cells to be sorted. Figure 2 The loading mechanism 30 and the selecting mechanism 20 are spaced apart along the third direction S3 and are disposed between the imaging mechanism 40 and the selecting mechanism 20 .

[0151] The carrying mechanism 30 is configured to be mounted on the main body 10. In some embodiments, the carrying mechanism 30 is connected to the support base 122 and is directly mounted on the main body 10. In some embodiments, the carrying mechanism 30 is connected to the imaging mechanism, which is mounted on the main body 10, thereby achieving indirect mounting of the carrying mechanism 30 on the main body 10.

[0152] The carrying mechanism 30 can move the parts to be sorted to a preset position so that the cells that meet the preset requirements can be sucked and recovered under the action of the selection mechanism 20. The carrying mechanism 30 includes a carrying assembly 31 and a carrying and moving assembly 32. The carrying assembly 31 is used to carry the parts to be sorted, and the carrying assembly 31 is placed on the carrying and moving assembly 32. The carrying and moving assembly 32 can drive the carrying assembly 31 to move so that the cells that meet the preset requirements in the parts to be sorted are spaced apart from the suction and release member 21 along the third direction S3. The setting of the carrying mechanism 30 realizes the placement and movement of the parts to be sorted, so that the cells that meet the preset requirements can be sucked and recovered, thereby improving the efficiency of cell sorting.

[0153] In some embodiments, see Figure 21 , illustrates the carrying assembly of the carrying mechanism 30 in some embodiments of the present application. The carrying assembly 31 includes a first carrying member 311 and a second carrying member 312. The first carrying member 311 and the second carrying member 312 are spaced apart along a third direction S3. The first carrying member 311 is movably connected to the second carrying member 312. The first carrying member 311 moves relative to the second carrying member 312 along the first direction S1, and the second carrying member 312 drives the first carrying member 311 to move along the second direction S2.

[0154] The first carrier 311 is used to accommodate the placement of the to-be-sorted items containing cells to be sorted. The first carrier 311 can be driven by the carrier moving assembly 32 to move relative to the base 121 of the main body 10 in a first direction S1. The second carrier 312 is used to place the first carrier 311. The second carrier 312 can be driven by the carrier moving assembly 32 to move relative to the base 121 of the main body 10 in a second direction S2, so that the to-be-sorted items are moved relative to the base 121 of the main body 10 to a preset area, thereby achieving the aspiration and recovery of cells that meet preset requirements in the preset area of ​​the to-be-sorted items. It is understood that the movement directions of the first carrier 311 and the second carrier 312 can be set according to actual needs and are not limited here. For example, the first carrier 311 can be driven by the carrier moving assembly 32 to move in the second direction S2, and the second carrier 312 can be driven by the carrier moving assembly 32 to move in the first direction S1.

[0155] See Figure 2 and Figure 22, shows the first carrier of some embodiments of the present application. The first carrier 311 is provided with a carrier portion 3111. On the one hand, the carrier portion 3111 can be used to place the objects to be sorted. On the other hand, the imaging mechanism 40 can use the carrier portion 3111 to image the cells to be sorted placed in the objects to be sorted to obtain image information of the cells to be sorted. In order to make full use of the space of the first carrier 311 and improve the sorting efficiency of the cells to be sorted, the first carrier 311 is provided with a preset number of carrier portions 3111 to place a preset number of objects to be sorted. For example, the first carrier 311 is provided with three carrier portions 3111 spaced apart along the second direction S2 to sort the cells to be sorted placed in the three objects to be sorted.

[0156] In some embodiments, see Figure 21 The carrier assembly 31 further includes a third carrier 313 for carrying the first carrier 311 and the second carrier 312 and is spaced apart from the second carrier 312 along the third direction S3. The second carrier 312 moves relative to the third carrier 313 along the second direction S2.

[0157] In some embodiments, see Figure 21 The carrier assembly 31 further includes a storage unit 314 disposed on the first carrier 311. The storage unit 314 is used to store cells that meet the preset requirements, so as to facilitate the subsequent cultivation of cells that meet the preset requirements. Figure 2 The cells that meet the preset requirements can be placed in the storage unit 314 after being sucked by the selection mechanism 20, and then the storage unit 314 is transferred to the incubator to carry out the next step of culturing the cells that meet the preset requirements.

[0158] The storage unit 314 is generally a porous structure. The porous structure allows for the recovery and placement of multiple cells, improving the efficiency of cell sorting and increasing the success rate and accuracy of cell sorting. For example, the storage unit 314 may be configured with 96 wells. It should be noted that the number of wells in the storage unit 314 can be set based on actual needs and is not limited herein.

[0159] See Figure 25 , Figure 25The storage unit in some embodiments of the present application is shown. The storage unit 314 includes a support frame 3141 and a reaction tube 3142. The support frame 3141 can be reused, and the reaction tubes 3142 are arranged on the support frame 3141 at intervals along the second direction S2, and can be recovered and cultured separately. Preferably, the distances between the reaction tubes 3142 are the same. For example, the support frame 3141 has 8 reaction tubes 3142 equidistantly arranged along the first direction S1, and 12 reaction tubes 3142 equidistantly arranged along the second direction S2. It should be noted that as long as the recovery and culture of cells can be achieved, the material of the storage unit 314 is not limited, and different materials can be selected according to actual needs. For example, the materials of the support frame 3141 and the storage portion 3142a component can be the same or different.

[0160] The support frame 3141 is provided with a through hole, and the reaction tube 3142 passes through the through hole to be combined with the support frame 3141. In order to facilitate the placement of the support frame 3141 and the determination of the position of the storage portion 3142a component, one of the corners of the support frame 3141 is set as a positioning chamfer. The other corners of the support frame 3141 except the positioning chamfer are set as rounded corners. The setting of the rounded corners can prevent the corners of the support frame 3141 from being sharp, which is convenient for the recovery and cultivation of the storage unit 314. The support frame 3141 is circumferentially provided with a skirt structure extending along the third direction S3. The setting of the skirt structure can strengthen the structural strength of the support frame 3141 itself and prevent the support frame 3141 from twisting and deforming. Furthermore, the skirt structure is stepped to facilitate the placement of the support frame 3141.

[0161] See Figure 26 , Figure 26 Schematic diagram of the reaction tube 3142 in some embodiments of the present application is shown. The reaction tube 3142 includes a receiving portion 3142a and a placement platform 3142b for placing the receiving portion 3142a. The placement platform 3142b is provided with a placement hole corresponding to the receiving portion 3142a. The setting of the receiving portion 3142a provides a place for accommodating and culturing cells in accordance with preset requirements. Figure 24 The placement holes on the placement platform 3142b and the through holes of the support frame 3141 all correspond to the receiving portions 3142a one by one. The number of the receiving portions 3142a matches the number of the through holes.

[0162] One end of the receiving portion 3142a extends along the third direction S3, and the size of at least a portion of the tube gradually increases along the third direction S3, forming a conical shape. The other end is open to accommodate cells that meet the preset requirements. The conical design facilitates cell recovery. In some embodiments, the receiving portion 3142a is transparent, facilitating subsequent observation of cells that meet the preset requirements.

[0163] The placement platform 3142b is in the shape of a square sheet. Positioning holes are provided at both ends of the placement platform 3142b along the first direction S1, but not on the same axis. This arrangement facilitates determining the positions of the different receiving portions 3142a. In some embodiments, the placement platform 3142b and receiving portions 3142a are injection molded separately and then thermally bonded to form the reaction tube 3142.

[0164] Specifically in the embodiment, each reaction tube 3142 includes a preset number of storage portions 3142a arranged in the first direction S1. The number of reaction tubes 3142 to be installed can be selected according to specific usage requirements, which is conducive to improving the flexibility of use of the storage unit 314, and is also conducive to further reducing consumables and avoiding waste. For example, the support frame 3141 has 96 mounting holes. If a certain experiment requires the use of 32 storage portions 3142a, only 4 reaction tubes 3142 can be installed in the support frame 3141. It should be noted that the preset number of storage portions 3142a in each reaction tube 3142 can also be set to other arrangements, such as a plurality of storage portions 3142a array distribution or a plurality of storage portions 3142a rectangular distribution, etc.

[0165] See Figures 19 to 22 , a placement groove 3112 for placing the storage unit 314 is provided on the first carrier 311. The storage unit 314 is placed on the first carrier 311 through the placement groove 3112, so that the storage unit 314 is placed and fixed. Specifically, the storage unit 314 is placed on the first carrier 311 by cooperating with the placement groove 3112 through the step skirt structure of the support frame 3141. The shape and size of the placement groove 3112 match the shape and size of the support frame 3141. Because the storage unit 314 and the parts to be sorted are spaced apart along the first direction S1, correspondingly, the placement groove 3112 provided on the first carrier 311 is spaced apart from the carrier 3111 along the first direction S1. It can be understood that as long as the absorption and recovery of cells that meet the preset requirements can be achieved, the positions of the parts to be sorted and the storage unit 314 are not limited, such as the parts to be sorted and the storage unit 314 are arranged at intervals along the second direction S2 or the storage unit 314 is arranged at intervals along the circumference of the parts to be sorted.

[0166] Furthermore, in order to better fix the first carrier 311, the first carrier 311 is further provided with an elastic buffer 3113, which is provided at at least one corner of the placement groove 3112, and the storage unit 314 is fixed to the first carrier 311 through the elastic buffer 3113. Figure 25 and Figure 26To prevent the storage portion 3142a from contacting the first carrier 311 and affecting the recovery of cells that meet the preset requirements, the first carrier 311 is provided with a through hole that communicates with the placement groove 3112. The through hole is smaller than the placement groove 3112, forming a step between the through hole and the placement groove 3112 to facilitate the placement of the storage unit 314. The size of the through hole is not limited as long as the storage portion 3142a can be properly placed.

[0167] See again Figure 19 and Figure 20 The loading and moving component 32 can drive the loading component 31 to move, thereby driving the to-be-sorted parts to move, so that the cells that meet the preset requirements in the to-be-sorted parts can be sucked, and then driving the storage unit 314 to move, so that the sucked cells that meet the preset requirements can be released into the storage unit 314. Figure 2 The control mechanism 60 can carry the moving component 32 to move and control the selection mechanism 20 to absorb and release, thereby achieving the absorption and release of cells that meet the preset requirements.

[0168] In some embodiments, the carrying and moving assembly 32 includes a first carrying and moving unit 321 and a second carrying and moving unit 322. The first carrying and moving unit 321 is used to drive the carrying assembly 31 to move along the first direction S1, and the second carrying and moving unit 322 is used to drive the carrying assembly 31 to move along the second direction S2. Specifically, the first carrying and moving unit 321 is used to drive the first carrier 311 to move along the first direction S1, and the second carrying and moving unit 322 is used to drive the second carrier 312 to move along the second direction S2.

[0169] In some embodiments, the first carrying and moving unit 321 includes a first carrying driving member 3211, a first carrying transmission member 3212, and a first carrying and moving member 3213. The first carrying driving member 3211 can drive the first carrying transmission member 3212 to rotate, thereby enabling the first carrying and moving member 3213 to move in the first direction S1. The first carrying and moving member 3213 is connected to the first carrying member 311, and the first carrying transmission member 3212 extends along the first direction S1. Driven by the first carrying driving member 3211, the first carrying and moving member 3213 drives the first carrying member 311 to move along the first direction S1.

[0170] Specifically, the first carrier driver 3211 can be a stepper motor to achieve quantitative movement of the first carrier 311, thereby controlling the first carrier 311 and the to-be-sorted components and the storage unit 314 disposed on the first carrier 311 to move a predetermined distance in the first direction S1. It will be appreciated that the type of the first carrier driver 3211 is not limited as long as it can achieve movement of the carrier assembly 31 along the first direction S1.

[0171] Preferably, the first carrier drive member 3211 utilizes a five-phase stepper motor. This motor enables high-precision positioning of the first carrier member 311. The static angle error during microstepping control is essentially the same as that achieved with full-step drive, resulting in high precision. Compared to other stepper motors, five-phase stepper motors also offer low vibration and low noise. This facilitates high-precision automated movement of the carrier assembly 31.

[0172] In some embodiments, to facilitate movement of the first carrier 311 along the first direction S1, the first carrier movement unit 321 further includes a first carrier guide 3214. The first carrier guide 3214 extends along the first direction S1 to enable movement of the first carrier 311 along the first direction S1. The first carrier guides 3214 are spaced apart on either side of the second carrier 312 along the second direction S2. The spaced apart first carrier guides 3214 ensure smooth movement of the first carrier 311 along the first direction S1. It should be noted that the extension lengths of the first carrier transmission member 3212 and the first carrier guide 3214 are determined by the travel range of the first carrier 311.

[0173] To facilitate the installation of the first loading guide 3214, refer to Figure 23 , illustrates the second carrier and first carrier movement unit in some embodiments of the present application. The second carrier 312 is provided with a first carrier guide groove 3121, and a first carrier guide 3214 is disposed within the first carrier guide groove 3121 to ensure smooth movement of the first carrier 311. It is understood that the first carrier guide 3214 can also be disposed on the surface of the second carrier 312.

[0174] In some embodiments, the first carrying and moving unit 321 further includes a first carrying slide 3215. The first carrying slide 3215 is disposed on the first carrying guide 3214 and is connected to the first carrying member 311. The first carrying slide 3215 slides on the first carrying guide 3214, driving the first carrying member 311 to move along the first direction S1. The provision of the first carrying slide 3215 reduces the loss during the movement of the first carrying member 311, and helps to improve the movement stability and displacement accuracy of the first carrying member 311. Furthermore, each first carrying guide 3214 is provided with a preset number of first carrying slides 3215, and the first carrying slides 3215 are arranged at intervals on the first carrying guide 3214 along the first direction S1. The preset number of first carrying slides 3215 further ensures the stability of the first carrying member 311 during movement.

[0175] In some embodiments, the first loading and moving unit 321 further includes a displacement sensor (not shown). The provision of the displacement sensor can obtain positional information of the items to be sorted and the storage unit 314, enabling the control mechanism 60 to automatically control the movement of the loading assembly 31, thereby improving cell sorting efficiency. Specifically, the displacement sensor is a grating displacement sensor. Grating displacement sensors have high measurement accuracy and resolution, good stability, strong anti-interference capabilities, are easy to interface with a microcomputer, and are suitable for long-distance transmission. It will be understood that the type of displacement sensor is not limited as long as it can obtain positional information of the loading assembly 31.

[0176] In some embodiments, see Figure 19 and Figure 20 The second carrying and moving unit 322 includes a second carrying driving member 3221, a second carrying transmission member 3222, and a second carrying and moving member 3223. The second carrying driving member 3221 is capable of driving the second carrying transmission member 3222 to rotate, thereby realizing the movement of the second carrying and moving member 3223 in the second direction S2. The second carrying and moving member 3223 is connected to the second carrying member 312, and the second carrying transmission member 3222 extends along the second direction S2. Under the drive of the second carrying driving member 3221, the second carrying member 312 is realized to move along the second direction S2.

[0177] Specifically, the second carrier drive member 3221 is a stepper motor, which realizes the quantitative movement of the second carrier member 312, thereby controlling the movement of the parts to be sorted and the storage unit 314 in the second direction S2 by a preset distance. It can be understood that as long as the movement of the carrier component 31 along the second direction S2 can be achieved, the type of the second carrier drive member 3221 is not limited. Preferably, the second carrier drive member 3221 is also a five-phase stepper motor. The setting of the five-phase stepper motor is more conducive to achieving high-precision automatic movement of the carrier component 31. The movement accuracy of the carrier component 31 must meet the movement requirements of the parts to be sorted, that is, the absorption of adjacent cells that meet the preset requirements is not interfered with, such as the movement accuracy of the carrier component 31 is 2 microns.

[0178] In some embodiments, the second carrier movement unit 322 further includes second carrier guides 3224. The second carrier guides 3224 extend along the second direction S2 and are spaced apart along the first direction S1 on the third carrier 313. The second carrier guides 3224 facilitate movement of the second carrier 312. It should be noted that the extension lengths of the second carrier transmission member 3222 and the second carrier guides 3224 are determined by the travel range of the second carrier 312.

[0179] See Figure 24, illustrates the third carrier and second carrier movement unit in some embodiments of the present application. The third carrier 313 is provided with a second carrier guide groove 3131, and the second carrier guide 3224 is disposed within the second carrier guide groove 3131 to ensure smooth movement of the second carrier 312. It is understood that the second carrier guide 3224 and the second carrier guide groove 3131 can also be disposed in reverse, i.e., the second carrier guide 3224 is disposed on the third carrier 313, and the second carrier guide groove 3131 is disposed on the second carrier.

[0180] In some embodiments, the second carrier movement unit 322 further includes a second carrier slide 3225. The second carrier slide 3225 is disposed on the second carrier guide 3224 and connected to the second carrier 312. The second carrier slide 3225 slides on the second carrier guide 3224, thereby driving the second carrier 312 to move, reducing wear on the second carrier 312 during movement and improving the stability and positioning accuracy of the second carrier 312 during movement. Furthermore, each second carrier guide 3224 is provided with a preset number of second carrier slides 3225, which are spaced apart along the first direction S1 on the second carrier guide 3224. The preset number of second carrier slides 3225 further ensures the stability of the second carrier 312 during movement.

[0181] In some embodiments, the second carrier-moving unit 322 further includes a displacement sensor. The displacement sensor is a proximity displacement sensor. Proximity displacement sensors have a simple structure, are stable and reliable, and offer high measurement accuracy and sensitivity. It should be understood that the type and installation location of the displacement sensor are not limited, as long as it can obtain displacement information of the second carrier 312.

[0182] See Figure 23 and Figure 24 The first loading and moving unit 321 is disposed on one side of the second loading member 312 in the second direction S2, and the second loading and moving unit 322 is disposed on one side of the third loading member 313 in the first direction S1. It should be noted that the installation positions of the first loading and moving unit 321 and the second loading and moving unit 322 are not limited as long as the first loading member 311 can move in the first direction S1 and the second loading member 312 can move in the second direction S2.

[0183] See again Figure 19 The loading mechanism 30 further includes a loading limit assembly 33. The loading limit assembly 33 is provided on the loading assembly 31 and is used to fix the object to be sorted on the loading assembly 31. The loading limit assembly 33 includes an elastic member 331 and a biasing connecting member 332. The elastic member 331 is configured to surround the surface of the object to be sorted and bias the object to be sorted in the radial direction of the object to be sorted. Figure 20The biasing connectors 332 are spaced apart on the first carrier 311 along the second direction S2, and the elastic member 331 is sleeved onto the biasing connectors 332. Specifically, in this embodiment, the biasing connectors 332 are arranged circumferentially around the receiving portion 3111 of the first carrier 311, so that the elastic member 331 can bias and limit the position of the object to be sorted placed on the first carrier 311. After the object to be sorted is placed on the first carrier 311, the elastic member 331 is pulled from one side of the receiving portion 3111 to the other side of the receiving portion 3111, biasing the object to be sorted so that it abuts against the receiving portion 3111, ensuring stability during placement and subsequent movement of the object to be sorted.

[0184] In some embodiments, the loading and limiting assembly 33 further includes a limiting member 333. The positioning of the limiting member 333 allows various sizes of items to be sorted to be fixed within the loading portion 3111. The shape and size of the inner diameter of the limiting member 333 are substantially consistent with the shape and size of the outer diameter of the item to be sorted, so that the outer wall of the item to be sorted fits against the inner wall of the limiting member 333. The shape and size of the outer diameter of the limiting member 333 are substantially consistent with the shape and size of the limiting groove in the loading portion 3111. The outer wall of the limiting member 333 fits into the limiting groove in the loading portion 3111, thereby limiting the position of the item to be sorted on the first loading portion 311.

[0185] Further to the embodiment, combined with Figure 22 The stopper 333 can be made of silicone. The stopper 333 increases friction between the object to be sorted and the receiving portion 3111, making the object more stable. The elastic member 331 can bias the stopper, providing a cushion for the object to be sorted, preventing or reducing damage to the object by the elastic member 331 and ensuring normal use of the object to be sorted. It is understood that the material, shape, and size of the stopper 333 can be set according to actual needs and are not limited here.

[0186] In other embodiments, the limiting member 333 may also be a pressing member, and a preset number of pressing members are spaced apart on the limiting groove of the loading portion 3111. By setting the pressing members, the position of the parts to be sorted in the loading portion 3111 is limited. The pressing members may be springs, spring pins, etc. When the parts to be sorted are placed in the loading portion 3111, the parts to be sorted press the pressing members in the loading portion 3111, and the pressing members are compressed and apply pressure on the parts to be sorted, thereby fixing the parts to be sorted in the loading portion 3111. It can be understood that the number and position of the pressing members can be set according to actual needs and are not limited here.

[0187] In some feasible embodiments, the position-limiting member 333 may be a fastener, which is provided on the inner wall of the receiving portion 3111 to secure the object to be sorted in the receiving portion 3111. The position-limiting member may also be a clamping member, which is provided on the circumference of the receiving portion 3111 to secure the object to be sorted in the receiving portion 3111. It is understood that the combination of the position-limiting member and the object to be sorted is not limited as long as the object to be sorted can be secured in the receiving portion 3111.

[0188] When the loading mechanism 30 is working, the parts to be sorted are placed on the loading component 31, and the parts to be sorted are limited by the loading limit component 33. The loading moving component 32 drives the parts to be sorted to move to achieve the absorption of cells that meet the preset requirements, and then drives the storage unit 314 to move to achieve the recovery and cultivation of cells that meet the requirements.

[0189] Specifically, when aspirating cells that meet the preset requirements, under the control of the control mechanism 60, the first loading and moving unit 321 drives the objects to be sorted to move in the first direction S1, and the second loading and moving unit 322 drives the objects to be sorted to move in the second direction S2, until the cells that meet the preset requirements are spaced apart from the suction and placement member 21 in the third direction S3. The selection moving assembly 23 drives the suction and placement member 21 to move to a preset position, and the drive unit 251 drives the suction and placement member 21 to aspirate the cells that meet the preset requirements into the hollow tube 211, completing the aspiration of the cells that meet the preset requirements.

[0190] Afterwards, the selection movement assembly 23 drives the suction and placement opening 212 of the suction and placement member 21 upward, the first loading movement unit 321 drives the storage unit 314 to move in the first direction S1, and the second loading movement unit 322 drives the storage unit 314 to move in the second direction S2, until the corresponding storage portion 3142a is spaced apart from the suction and placement member 21 in the third direction S3. The selection movement assembly 23 drives the suction and placement member 21 to move to a preset position, and the drive unit 251 drives the suction and placement member 21 to release cells that meet the preset requirements into the storage unit 314, completing the recovery and cultivation of cells that meet the preset requirements.

[0191] In some embodiments, see Figure 27 and Figure 28 , illustrates an imaging mechanism in some embodiments of the present application. Imaging mechanism 40 is used to image cells to be sorted and obtain image information of the cells to be sorted, thereby selecting cells that meet preset requirements. Imaging mechanism 40 includes an imaging illumination unit 41, an optical path assembly 42, a fluorescence assembly 43, and a detection assembly 44.

[0192] The imaging lighting unit 41 provides a bright field illumination source for the cells to be sorted and the optical path component 42, irradiating the cells to be sorted with transmitted light, which then passes through the optical path component 42 to the detection component 44, and the image information of the cells to be sorted is obtained through the detection component 44. Figure 2 and Figure 9 The imaging lighting unit 41 is spaced apart from the optical path component 42 along the third direction S3, and the parts to be sorted are arranged between the imaging lighting unit 41 and the optical path component 42 along the third direction S3, so that the transmitted light emitted by the imaging lighting unit 41 enters the optical path component 42 after passing through the parts to be sorted, thereby realizing the imaging of the cells to be sorted.

[0193] See again Figure 8 and Figure 9 The imaging lighting unit 41 is used to illuminate the cells to be sorted, providing a certain brightness for observation of the cells to be sorted. The imaging lighting unit 41 is connected to the mounting assembly 22 along the third direction S3. Figure 2 and Figure 9 , the imaging lighting unit 41 is connected to the accommodating member 223 along the third direction S3. The imaging lighting unit 41 is closer to the suction and placement port 212 of the suction and placement member 21 than the selection lighting unit 24. The imaging lighting unit 41 and the selection lighting unit 24 are spaced apart along the axial direction of the suction and placement member 21 and the third direction S3.

[0194] In some embodiments, the imaging lighting unit 41 includes an imaging light source 411, a light shielding member 412, and a light transmitting member 413. The light emitted by the imaging light source 411 passes through the light shielding member 412 and the light transmitting member 413 to the cells to be sorted, and illuminates the cells to be sorted along the periphery of the suction and release member 21. The imaging light source 411 is connected to the mounting assembly 22 along the third direction S3 so that the light can be transmitted to the imaging mechanism 40 to meet the lighting requirements of the imaging mechanism 40. It should be noted that the position of the imaging light source 411 is set according to actual conditions.

[0195] The imaging light source 411 is provided with a central hole for the suction and placement member 21 to pass through, so that the suction and placement member 21 can pass through the imaging light source 411. Figure 12 and Figure 13 The first clamping and driving member 2212 is connected to the imaging light source 411 through a central hole. The diameter of the central hole is slightly larger than the outer diameter of the second clamping and driving portion 2212c of the first clamping and driving member 2212. The central axis of the imaging light source 411 in the third direction S3 is aligned with or parallel to the central axis of the suction and placement member 21 in the third direction S3, facilitating illumination by the imaging light source 411.

[0196] In some embodiments, the imaging light source 411 is a ring light source that provides bright and uniform illumination for a large field of view. Further, the imaging light source 411 is an LED light source. The ring LED light source can be a whole ring light source, or it can be obtained by arranging multiple light sources evenly spaced circumferentially, so that the imaging light source 411 illuminates the cell area to be sorted from the radial periphery of the suction and placement member 21. It should be noted that the type of ring light source is not limited to an LED light source.

[0197] Further to some embodiments, see Figure 9 The imaging light source 411 and the receiving member 223 are detachably connected. This detachable connection facilitates installation and replacement of the suction and placement member 21, preventing the suction and placement member 21 from contacting the imaging light source 411 during installation and replacement, thereby damaging the suction and placement member 21. Furthermore, it facilitates installation and replacement of the imaging light source 411.

[0198] In some embodiments, when the cell sorting device 100 is used, the suction and placement component 21 is first installed on the clamping unit 221, and then the clamping unit 221 is placed in the receiving component 223, and finally the imaging lighting unit 41 is passed through the suction and placement component 21 along the third direction S3 and connected to the receiving component 223 to avoid the suction and placement component 21 hitting the imaging lighting unit 41 during installation, causing damage to the suction and placement component 21.

[0199] Specifically in the embodiment, the imaging light source 411 and the container 223 are magnetically connected. In one embodiment, a magnet is provided on the end face of the imaging light source 411, and the container 223 is made of metal. The light source cover and the container 223 are magnetically connected by magnets adsorbing the metal material. In a feasible embodiment, the container 223 is made of non-metallic material, and a magnetic metal part is provided on the container 223. The magnetic connection between the light source cover and the container 223 is achieved by magnets adsorbing the magnetic metal part. It can be understood that the positions of the magnets and the magnetic metal parts can be interchanged and the specific setting positions are not limited. For example, the light source cover is provided with a magnetic metal part, and the container 223 is provided with a magnet to achieve the connection between the light source cover and the container 223. The magnetic connection makes it easy to disassemble the connection between the mounting assembly 22 and the lighting assembly 24, and facilitates the installation and replacement of the suction and release part 21. At the same time, the magnetic connection does not destroy the structure of the mounting assembly 22 and the lighting assembly 24. It is reliable, stable, convenient and fast, and does not require precise alignment.

[0200] In an embodiment (not shown), the imaging light source 411 is provided with a groove, and the receiving member 223 is provided with a protrusion that mates with the groove. The combination of the groove and the protrusion achieves a detachable connection between the mounting assembly 22 and the lighting assembly 24. It should be understood that the connection between the imaging light source 411 and the receiving member 223 is not limited to a detachable connection, as long as the connection between the imaging light source 411 and the receiving member 223 is achieved. For example, the imaging light source 411 may be glued to the receiving member 223.

[0201] In some embodiments, the light-transmitting member 413 and the light-shielding member 412 jointly limit the scattering of light from the imaging light source 411. The light-shielding member 412 is arranged between the imaging light source 411 and the light-transmitting member 413 along the third direction S3. The light-shielding member 412 is a hollow cylindrical member as a whole and extends along the third direction S3 to enable the insertion of the suction and release member 21. The outer diameter of the light-shielding member 412 matches the outer diameter of the imaging light source 411. The light-transmitting member 413 is a transparent ring as a whole and is provided with a center hole. The outer diameter of the light-transmitting member 413 matches the outer diameter of the imaging light source 411. The shape and size of the center hole match the outer diameter shape and size of the suction and release member 21 for the insertion of the suction and release member 21. The light-transmitting member 413 can be made of an acrylic plate. It can be understood that as long as the scattering of light and the transmission of light can be limited, the shape, size and material of the light-shielding member 412 and the light-transmitting member 413 are not limited.

[0202] In some embodiments, the light-transmitting member 413, the light-blocking member 412, and the imaging light source 411 are detachably connected. The detachable connection facilitates maintenance and replacement. Specifically, the light-transmitting member 413, the light-blocking member 412, and the imaging light source 411 are all coaxially provided with threaded holes in the third direction S3, and the light-transmitting member 413, the opaque member 413, and the imaging light source 411 are connected by fasteners through the threaded holes. It should be noted that the imaging light source 411, the light-blocking member 412, and the light-transmitting member 413 are not limited to detachable connections, and the connection between the imaging light source 411, the light-blocking member 412, and the light-transmitting member 413 can also be achieved by gluing.

[0203] When imaging light source 411 is a transmitted light source, any light source that emits light containing wavelengths that are absorbed or reflected by the cells to be sorted can be used. Various lamps, such as halogen lamps, light-emitting diodes, and various lasers can be used as imaging light source 411. Images of the cells to be sorted can be brightfield or darkfield images. Furthermore, in some embodiments, the brightness of imaging light source 411 can be adjusted, making it applicable to a wider range of situations and facilitating brightness adjustment of image information.

[0204] In some embodiments, see Figure 28The optical path assembly 42 is used to form the optical path of the imaging mechanism 40. It is equipped with optical elements for forming a transmissive light path to achieve imaging of the cells to be sorted. The optical path assembly 42 includes an objective lens 421 and a first imaging lens 422. The objective lens 421 has a certain focal length. The preset area of ​​the cells to be sorted and the objective lens 421 are spaced apart along the third direction S3. The objective lens 421 images the cells to be sorted in the preset area. The objective lens 421 and the first imaging lens 422 are spaced apart along the third direction S3 to form the transmissive light path of the imaging mechanism 40. The transmissive light emitted by the imaging light source 411 passes through the cells to be sorted, the objective lens 421, and the first imaging lens 422 in sequence to the detection assembly 44. Specifically, the objective lens 421 can be a 10x objective lens 421. It should be noted that the magnification of the objective lens 421 can be set according to actual needs and is not limited here. In other embodiments, the optical path assembly 42 may include multiple objective lenses 421 to obtain image information of the cells to be sorted at different magnifications. Since the objective lens 421 and the first imaging lens 422 are arranged on one side of the cells to be sorted in the third direction S3, the light generated by the imaging light source 411 at the cells to be sorted may not be transmitted light but reflected light.

[0205] In some embodiments, see Figure 27 and Figure 28 The optical path assembly 42 further includes an objective lens moving unit 423. The objective lens moving unit 423 is used to focus the objective lens 421 and change the focal length between the objective lens 421 and the object to be sorted, thereby providing a clear image of the cells to be sorted and enabling the imaging mechanism 40 to obtain clear image information of the cells to be sorted. The objective lens moving unit 423 can drive the objective lens 421 to move relative to the object to be sorted in a third direction S3 to provide a clear image information of the cells to be sorted.

[0206] In some embodiments, the objective lens moving unit 423 includes a first rotating member 4231 and a second rotating member (not shown). The first rotating member 4231 and the second rotating member are arranged relative to each other along the second direction S2. The first rotating member 4231 and the second rotating member are both capable of moving the objective lens 421 along the third direction S3 to achieve focusing of the objective lens 421. The second rotating member can automatically move the objective lens 421 under the control of the control mechanism 60, while the first rotating member 4231 is not controlled by the control mechanism 60 and can move the objective lens 421 under the action of an external force. The arrangement of the first rotating member 4231 and the second rotating member allows the objective lens 421 to be focused both automatically and manually, thereby improving the applicability of the cell sorting apparatus 100.

[0207] In some embodiments, the objective lens movement unit 423 further includes an objective lens driver 4232. The objective lens driver 4232 is connected to the control mechanism 60 and is capable of driving the second rotating member to rotate, thereby moving the objective lens 421 along the third direction S3. This adjusts the distance between the objective lens 421 and the cells to be sorted to achieve focusing of the objective lens 421 and obtain high-definition image information of the cells to be sorted. The objective lens driver 4232 can utilize a stepping motor to precisely control the movement distance of the objective lens 421, enabling coarse and fine adjustments during the focusing process of the objective lens 421.

[0208] In some embodiments, the objective lens moving unit 423 further includes a displacement sensor (not shown). The provision of the displacement sensor facilitates control of the displacement of the objective lens 421, thereby facilitating automatic focusing of the objective lens 421. In this embodiment, the displacement sensor is a grating displacement sensor. It is understood that the type of displacement sensor is not limited as long as it can obtain position information of the carrier assembly 31.

[0209] In some embodiments, the objective lens moving unit 423 also includes an objective lens limiter not shown in the figure. The setting of the objective lens limiter limits the moving stroke of the objective lens 421. Combined with the displacement sensor, the highest position and the lowest position of the objective lens 421 can be obtained to prevent the objective lens 421 from colliding with the loading mechanism 30 and damaging the objective lens 421, thereby facilitating the automatic movement of the objective lens 421 to achieve focusing.

[0210] Combine Figures 19 to 22 The first carrier 311 is configured as a stepped structure in the third direction S3. On the one hand, it prevents the second carrier 312 from colliding with the storage unit 314. On the other hand, it provides a moving distance for the objective lens 421 to focus, preventing the objective lens 421 from colliding with the first carrier 311 during the focusing process, thereby damaging the objective lens 421. Furthermore, the mirror surface of the objective lens 421 is configured between the first carrier 311 and the second carrier 312 in the third direction S3 to better achieve the focusing of the objective lens 421 and ensure that the image information of the cells to be sorted is clear. The third carrier 313 is provided with an observation hole 3132 to facilitate the focusing of the objective lens 421 and prevent the objective lens 421 from colliding with the third carrier 313. The second carrier 312 is provided with a through hole for the focusing of the objective lens 421 to pass through. The size of the through hole should ensure that the movement of the second carrier 312 will not collide with the objective lens 421, thereby ensuring the normal use of the cell sorting device 100.

[0211] Combine Figure 2 In some embodiments, the objective lens 421 is aligned with or parallel to the central axis of the suction and placement member 21 in the third direction S3. The alignment of the objective lens 421 with or parallel to the central axis of the suction and placement member 21 is beneficial for improving the accuracy of the suction and placement member 21 in sucking cells that meet preset requirements, thereby improving the sorting efficiency of the cell sorting device 100.

[0212] See again Figure 27 and Figure 28 The fluorescent component 43 is used to make the cells to be sorted emit fluorescence, and the cells that meet the preset requirements are found through the fluorescence image information of the cells to be sorted. The fluorescent component 43 and the optical path component 42 are arranged on the same side of the cells to be sorted. The fluorescent component 43 includes an excitation light source 431 and a filter unit 432. The excitation light source 431 is used to irradiate the cells to be sorted and make the cells to be sorted emit fluorescence. The excitation light source 431 can be any light source that can make the cells to be sorted emit fluorescence. As the excitation light source 431, lamps such as xenon lamps, mercury lamps, various lasers such as argon lasers, and light-emitting diodes can be used.

[0213] The filter unit 432 is a core component of the imaging mechanism 40 for achieving fluorescence imaging. In some embodiments, the filter unit 432 is positioned between the objective lens 421 and the first imaging lens 422 along the third direction S3 and includes a first beam splitter 4321. The first beam splitter 4321 is capable of reflecting light emitted by the excitation light source 431 while allowing light and fluorescence transmitted through the cells to be sorted to pass through, thereby enabling the detection assembly 44 to obtain image information of the cells to be sorted. The first beam splitter 4321 can separate the wavelength of the imaging light source 411 from the wavelength of fluorescence generated by the cells to be sorted, such as by using a dichroic mirror or a dichroic mirror. The first beam splitter 4321 is positioned between the objective lens 421 and the first imaging lens 422 along the third direction S3. The first beam splitter 4321 is angled relative to the third direction S3 and spaced apart from the excitation light source 431 along the second direction S2 to reflect light from the excitation light source 431 toward the cells to be sorted, thereby causing the cells to emit fluorescence.

[0214] In some embodiments, the filter unit 432 further includes a first filter 4322. The first filter 4322 is disposed along the second direction S2 between the first beam splitter 4321 and the excitation light source 431. The first filter 4322 is configured to filter the excitation light source 431 so that light in a wavelength band that causes the cells to be sorted to emit fluorescence reaches the first beam splitter 4321.

[0215] The observation light path in the imaging mechanism 40 includes an illumination light path obtained by the light emitted by the imaging light source 411 passing through the cells to be sorted, a guiding light path that guides the light emitted by the excitation light source 431 to the cells to be sorted, and a fluorescence light path generated by the cells to be sorted.

[0216] In some embodiments, the fluorescence assembly 43 further includes a filter switching unit 433. The filter switching unit 433 is used to switch between different filter units 432. After passing through different filter units 432, excitation light of different wavelengths is obtained, causing the cells to be sorted to emit different fluorescence. This allows the imaging mechanism 40 to meet the culture requirements of the cells to be sorted in various situations, thereby increasing the applicability of the cell sorting device 100.

[0217] In some embodiments, cells to be sorted are specifically screened by adding culture medium containing predetermined fluorescent dyes. Accordingly, different fluorescent dyes can produce different fluorescence under the action of excitation light. For example, cells to be sorted are cultured with three fluorescent dyes. After the excitation light passes through the first filter unit 432, the cells to be sorted emit a first fluorescence. After the excitation light passes through the second filter unit 432, the cells to be sorted emit a second fluorescence. After the excitation light passes through the third filter unit 432, the cells to be sorted emit a third fluorescence.

[0218] In a specific embodiment, cells to be sorted are specifically screened by adding culture medium containing a fluorescent dye capable of producing blue fluorescence, a fluorescent dye capable of producing green fluorescence, and a fluorescent dye capable of producing red fluorescence. Short-wavelength light excites the production of long-wavelength light. Therefore, ultraviolet excitation produces blue light, blue light excitation produces green light, and green light excitation produces red light. Cells to be sorted that have been cultured with a fluorescent dye capable of producing blue fluorescence use an ultraviolet filter unit 432, where the excitation light causes the cells to emit blue fluorescence. Cells to be sorted that have been cultured with a fluorescent dye capable of producing green fluorescence use a blue light filter unit 432, where the excitation light causes the cells to emit green fluorescence. Cells to be sorted that have been cultured with a fluorescent dye capable of producing red fluorescence use a green light filter unit 432, where the excitation light causes the cells to emit red fluorescence. Specific screening of cells to be sorted using predetermined fluorescent dyes facilitates more accurate selection of cells that meet predetermined requirements. It is understood that different filter units 432 can be selected based on different fluorescent dyes, and the types of fluorescent dyes and filter units 432 are not limited.

[0219] In some embodiments, the filter switching unit 433 includes a switching driver 4331. The switching driver 4331 is connected to the filter unit 432 and can drive the filter unit 432 to move, thereby switching between different filter units 432. The switching driver 4331 is arranged on one side of the objective lens 421 along the second direction S2. The filter units 432 connected to the switching driver 4331 can be moved to the observation light path of the imaging mechanism 40, such as between the objective lens 421 and the first imaging lens 422 in the third direction S3. Figure 2 The control mechanism 60 can control the filter switching unit 433 to switch different filter units 432 to excite different fluorescent dyes to produce different fluorescence.

[0220] In some embodiments, the filter unit 432 is configured with a first filter 4322, a second filter, and a third filter. The first filter 4322, the second filter, and the third filter are different from each other. The filter switching unit 433 switches between the first filter 4322, the second filter, and the third filter. When the filter switching unit 433 switches to the first filter 4322, the light emitted by the excitation light source 431 passes through the first filter 4322, causing the cells to emit a first fluorescence. When the filter switching unit 433 switches to the second filter, the light emitted by the excitation light source 431 passes through the second filter, causing the cells to emit a second fluorescence. When the filter switching unit 433 switches to the third filter, the light emitted by the excitation light source 431 passes through the third filter, causing the cells to emit a third fluorescence. It should be noted that the number of filters can be set based on the desired fluorescence emitted by the cells to be sorted.

[0221] In some embodiments, the filter switching unit 433 includes a switching driver 4331 connected to the control mechanism 60. The switching driver 4331 is connected to the excitation light source 431, and the cells to be sorted can emit different fluorescence by switching the excitation light source 431. For example, three groups of excitation light sources 431 that can excite different fluorescence can be switched into the observation light path, and different excitation light sources 431 emit excitation light of different wavelengths, such as ultraviolet LED light sources, blue LED light sources, green LED light sources, etc., so that the cells to be sorted can emit different fluorescence. It should be noted that the filter switching unit 433 can also switch different light sources in and out of the light path, switch different filter units 432, or switch different filters in and out of the light path. It can also be switched manually or automatically. The specific setting method can be set according to actual needs, and the switching method of the excitation light source 431 is not limited here.

[0222] In some embodiments, the filter unit 432 further includes a fourth filter 4323. The fourth filter 4323 is disposed along the third direction S3 between the first beam splitter 4321 and the first imaging lens 422. The fourth filter 4323 is configured to filter the fluorescence emitted by the cells to be sorted so that the fluorescence image information of the cells to be sorted reaching the detection component 44 is not affected by other light.

[0223] The filter unit 432 plays a crucial role in fluorescence imaging in the imaging mechanism 40. Light emitted from the excitation light source 431 strikes the first filter 4322, which selects a specific wavelength band of the excitation light source 431 to excite the sample and blocks light of other wavelengths. The light passing through the first filter 4322 passes through the first beam splitter 4321, which reflects the filtered excitation light. After reflection, the light is focused by the objective lens 421 and illuminates the cells to be sorted, causing them to emit corresponding fluorescence. The fluorescence is collected by the objective lens 421, passes through the first beam splitter 4321, and reaches the fourth filter 4323. When the first beam splitter 4321 is tilted 45 degrees relative to the excitation light source 431, the filtered excitation light is directed perpendicularly to the objective lens 421, which then acts as a direct condenser. In a feasible embodiment, the first filter element 4322 , the fourth filter element 4323 and the first beam splitter 4321 are integrated to obtain the filter unit 432 .

[0224] See Figure 28 The detection component 44 is connected to a display mechanism 50 for image observation and processing. The detection component 44 is used to detect the image information formed by the transmitted light and fluorescence of the cells to be sorted emitted by the optical path component 42, and process the obtained image information, and transmit the obtained image information to the display mechanism 50. In one embodiment, the detection component 44 includes a CCD sensor 441. In another embodiment, the detection component 44 includes a CMOS sensor 441. The CMOS sensor 441 or the CCD sensor 441 can both obtain bright field image information and fluorescence image information of the cells to be sorted. This facilitates subsequent processing and screening. Furthermore, the detection component 44 also includes a cooling unit (not shown), which cools the CMOS sensor 441 or the CCD sensor 441 by using a cooling unit such as liquid nitrogen, a Peltier element, etc., so as to improve the signal-to-noise ratio and reduce the noise of the image information.

[0225] In some embodiments, the detection assembly 44 includes a brightfield image information detector 442 and a fluorescence image information detector 443. The brightfield image information detector 442 is used to detect image information based on the transmitted light and reflected light of the imaging light source 411 passing through the cells to be sorted. The fluorescence image information detector 443 is used to detect fluorescence image information emitted by the cells to be sorted. Both the brightfield image information detector 442 and the fluorescence image information detector 443 are connected to the CMOS sensor 441 or the CCD sensor 441.

[0226] In some embodiments, the optical path assembly 42 is provided with a second beam splitter 424 and a second imaging lens 425. The second beam splitter 424 is spaced apart from the first beam splitter 4321 along the third direction S3 and is disposed between the first beam splitter 4321 and the first imaging lens 422. Transmitted light from the microscopic cells that has passed through the first beam splitter 4321 passes through the second beam splitter 424 and the first imaging lens 422, and is then emitted to the bright-field image information detector 442. Furthermore, a filter (not shown) is disposed between the first imaging lens 422 and the bright-field image information detector 442 to prevent the transmitted light from interfering with the excitation light source 431 or fluorescence and thereby entering the bright-field image information detector 442.

[0227] The second imaging lens 425 is disposed between the second beam splitter 424 and the fluorescence image information detector 443. Fluorescence from the cells to be sorted, which has passed through the first beam splitter 4321, is reflected by the second beam splitter 424 and passes through the second imaging lens 425 before being emitted to the fluorescence image information detector 443. Furthermore, a filter (not shown) is disposed between the imaging lens and the fluorescence image information detector 443 to prevent the fluorescence from interfering with the excitation light source 431 or the transmitted light and entering the fluorescence image information detector 443.

[0228] The first imaging lens 422 used by the brightfield image information detector 442 and the second imaging lens 425 used by the fluorescence image information detector 443 can be the same lens or different lenses. For example, the magnification of the first imaging lens 422 used by the brightfield image information detector 442 can be reduced, while the magnification of the second imaging lens 425 used by the fluorescence image information detector 443 can be increased. In this case, the field of view of the transmitted light image acquired by the brightfield image information detector 442 can be expanded, and a more enlarged fluorescence image of the cells to be sorted can be obtained by the fluorescence image information detector 443.

[0229] In some embodiments, combined Figure 2 and Figure 27 The imaging mechanism 40 also includes a protective housing 45, which protects the internal components of the imaging mechanism 40 and protects the optical path of the imaging mechanism 40 from external light, ensuring accurate and clear images of the cells to be sorted. The protective housing 45 is connected to the base plate 1211 via fasteners. The base plate 1211 is also provided with fixing bumps for securing the protective housing 45. The protective housing 45 is restrained by multiple fixing bumps, ensuring stable placement of the imaging mechanism 40.

[0230] Before using the imaging mechanism 40, the imaging illumination unit 41, the cells to be sorted, and the objective lens 421 are arranged along the third direction S3. During use, brightfield image information of the cells to be sorted is obtained by the following specific operations: the imaging illumination unit 41 is turned on, and light emitted by the imaging light source 411 passes through the cells to be sorted, the objective lens 421, the filter unit 432, and the first imaging lens 422 and enters the brightfield image information detector 442. The brightfield image information detector 442 transmits the acquired image information of the cells to be sorted to the display mechanism 50. Based on the image information displayed by the display mechanism 50, the objective lens movement unit 423 is controlled to move the objective lens 421 along the third direction S3 to make the image information of the cells to be sorted clear. The image information is then recorded by the CMOS sensor 441 or a CMOS sensor for subsequent processing.

[0231] Obtaining fluorescent image information of the cells to be sorted is performed as follows: Turn off the imaging light source 411 and turn on the excitation light source 431. Light emitted by the excitation light source 431 passes through the filter unit 432 and the objective lens 421 to the cells to be sorted, causing the cells to be sorted, which have been specifically screened for fluorescent dyes, to emit fluorescence. The fluorescence passes through the objective lens 421, the filter unit 432, and the second imaging lens 425 and enters the fluorescence image information detector 443. The fluorescence image information detector 443 transmits the fluorescence image information of the cells to be sorted to the display mechanism 50. The image information obtained by the display mechanism 50 is used to control the objective lens moving unit 423 to move the position of the objective lens 421 to make the image information clear. The image information is then recorded by the CCD sensor 441 or CMOS sensor. A different filter unit 432 is replaced by the filter switching unit 433. The above operation is repeated to transmit different fluorescence image information of the cells to be sorted to the display mechanism 50. By analyzing the acquired brightfield image information and fluorescence image information of the cells to be sorted, cells that meet the preset requirements are identified.

[0232] It should be noted that the switching between the imaging light source 411 and the excitation light source 431 can be achieved through the control mechanism 60 or through manual switching.

[0233] The cells to be sorted can be automatically moved under the control of the control mechanism 60. The fluorescent assembly 43 is equipped with different filter units 432, which are automatically switched by the filter switching unit 433. The objective lens movement unit 423 achieves automatic focusing, enabling automated scanning by the imaging mechanism 40. This allows for automatic scanning and imaging of large numbers of cells to be sorted, greatly improving the efficiency of the cell sorting apparatus 100 and expanding its application range. Furthermore, by comparing and analyzing multiple fluorescent image information and brightfield image information before and after sorting, more accurate cell sorting can be achieved.

[0234] See Figure 29In some embodiments, the control mechanism 60 is used to control the cell sorting device 100 to achieve automatic cell sorting, improve the efficiency of cell sorting, and achieve high-throughput sorting. The control mechanism 60 can control the selection drive 231, thereby controlling the movement and stop of the suction and release member 21 in the third direction S3; the control mechanism 60 can control the opening and closing of the selection light source 241 to achieve observation and alignment of the suction and release member 21; the control mechanism 60 can control the drive unit 251 to achieve cleaning of the suction and release member 21 and control the suction and release member 21 to absorb or release cells that meet preset requirements; the control mechanism 60 can control the first carrying drive 3211 and the second carrying drive 3221, thereby controlling the movement and stop of the carrying assembly 31; controlling The mechanism 60 can control the on and off of the imaging light source 411, provide a transmitted light source for the cells to be sorted, and obtain bright field image information of the cells to be sorted; the control mechanism 60 can control the on and off of the excitation light source 431, provide an excitation light source 431 for the cells to be sorted, and obtain fluorescence image information of the cells to be sorted; the control mechanism 60 can control the objective lens driver 4232 to achieve the focusing of the objective lens 421 and obtain clear image information of the cells to be sorted; the control mechanism 60 can control the switching driver 4331 to excite the cells to be sorted to emit different fluorescence.

[0235] In some embodiments, the control mechanism 60 is disposed on the main body 10 and includes a power module, a switch module, a switching module, a voltage module, etc. (not shown). It is understood that any component used to control the functional components of the cell sorting apparatus 100 is included in the control mechanism 60 . The control mechanism 60 can select corresponding control elements based on the configuration of the components of the cell sorting apparatus 100 .

[0236] In some embodiments, the cell sorting apparatus 100 further includes an illumination assembly. This illumination assembly is used to illuminate the hollow conduit 211 of the suction and placement member 21 and the imaging mechanism 40, thereby enabling observation and alignment of the suction and placement member 21 on the display mechanism 50. The illumination assembly includes one or both of the aforementioned sorting illumination unit 24 and imaging illumination unit 41.

[0237] In some embodiments, the method for operating the cell sorting device 100 includes the following steps:

[0238] Install the suction and release member 21 to the installation assembly 22. Specifically, first move the first shell 111 and open the cell sorting device 100. Loosen the first fixing member 2221 and the second fixing member 2222, remove the clamping unit 221, and loosen the first clamping drive member 2212. Be careful not to completely separate the first clamping drive member 2212 from the clamping body 2211. Place the end of the suction and release member 21 that is opposite to the suction and release port 212 into the clamping unit 221, tighten the first clamping drive member 2212 to the clamping mark 2218, and do not tighten it forcefully to cause damage to the suction and release member 21. Put the clamping unit 221 to which the suction and release member 21 is fixed back into the accommodating member 223. Tighten the first fixing member 2221 and the second fixing member 2222, fix the clamping unit 221, and complete the installation of the suction and release member 21 to the installation assembly 22.

[0239] Demarcate the position of the suction and release member 21. Specifically, under the control of the control mechanism 60, turn on the selection light source 241, select the mobile assembly 23 to drive the suction and release member 21 to move along the third direction S3, until the image information of the suction and release member projection is clearly presented on the display mechanism 50. Use the first fixing member 2221 and the second fixing member 2222 to fine-tune the suction and release member 21 so that the projection mark is located at the preset position of the suction and release member projection mapping. Afterwards, the to-be-sorted member that is not provided with the cells to be sorted is placed in the fixing groove of the first carrier 311. Select the mobile assembly 23 to drive the suction and release member 21 to move along the third direction S3, until the image information of the suction and release port 212 is clearly presented on the display mechanism 50. Record the position information of the suction and release member 21 at this time, and complete the position calibration of the suction and release member 21.

[0240] Clean the suction and release component 21 for the first time. Specifically, first take the first holding component 2551, add an appropriate amount of the first cleaning liquid 2541, and place the first holding component 2551 with the first cleaning liquid 2541 on the first carrier 311, so that the first holding component 2551 and the suction and release component 21 are spaced apart along the third direction S3. Under the control of the control mechanism 60, the driving component 231 is selected to drive the suction and release port 212 of the suction and release component 21 to move into the first cleaning liquid 2541, and the driving unit 251 drives the suction and release component 21 to absorb the first cleaning liquid 2541 into the hollow pipe 211 of the suction and release component 21 for cleaning, and then drives the suction and release component 21 to release the first cleaning liquid 2541 into the first holding component 2551, and repeats several times to complete the first step of cleaning for the first preset number of times. Then take the second holding component 2552, add an appropriate amount of second cleaning liquid 2542, place the second holding component 2552 with the second cleaning liquid 2542 on the first supporting component 311, so that the second holding component 2552 and the suction and release component 21 are placed at intervals along the third direction S3, select the driving component 231 to drive the suction and release component 21 to move into the second cleaning liquid 2542, the driving unit 251 drives the suction and release component 21 to absorb the second cleaning liquid 2542 into the hollow pipe 211 of the suction and release component 21 for cleaning, and then drive the suction and release component 21 to release the second cleaning liquid 2542 into the second holding component 2552, repeat several times, and complete the second step of cleaning for the second preset number of times.

[0241] Place the objects to be sorted and the storage unit 314. Place the objects containing the cells to be sorted in the loading section 3111 of the first loading member 311. Pull the elastic member 331 on one side of the loading section 3111 to the other side of the loading section 3111, biasing the elastic member 331 against the objects to be sorted, thereby placing and securing the objects to be sorted on the first loading member 311. Place the storage unit 314, containing an appropriate amount of culture medium, in the placement slot 3112 of the first loading member 311. The cells to be sorted undergo specific screening using a culture medium containing a fluorescent dye.

[0242] Find cells that meet the preset requirements. Under the control of the control mechanism 60, the imaging light source 411 is turned on, the first loading and moving unit 321 and the second loading and moving unit 322 drive the cells to be sorted to be observed in the sorted part to be arranged along the third direction S3 with the second light source and the objective lens 421, and the objective lens moving unit 423 drives the objective lens 421 to move until the image information of the cells to be sorted to be observed is clearly displayed on the display mechanism 50, and the bright field image information of the cells to be sorted is recorded. The imaging light source 411 is turned off, the excitation light source 431 is turned on, and the light emitted by the excitation light source 431 passes through the first filter unit 432, causing the cells to be sorted to emit a first fluorescence, and the first fluorescence image information of the cells to be sorted is recorded. The filter switching unit 433 is controlled to switch to the second filter unit 432, causing the cells to be sorted to emit a second fluorescence, and the second fluorescence image information of the cells to be sorted is recorded. The filter switching unit 433 is controlled to switch to the third filter unit 432, causing the cells to be sorted to emit a third fluorescence, and the third fluorescence image information of the cells to be sorted is recorded. Then, the excitation light source 431 is turned off and the imaging light source 411 is turned on. The first and second carrier-moving units 321 and 322 drive the cells to be sorted to a predetermined area, where the cells to be sorted are replaced. The above process is repeated, recording brightfield and fluorescence images of the cells to be sorted in different areas. The acquired brightfield and fluorescence images of the cells to be sorted are processed and analyzed to identify cells that meet the predetermined criteria.

[0243] Absorb and recover cells that meet the preset requirements. Under the control of the control mechanism 60, the selection moving component 23 drives the suction and release component 21 to move along the third direction S3 until the suction and release component 21 reaches a position where it can absorb cells. The position is marked as the preset position, and pre-absorption is completed. The second loading moving unit 322 drives the second loading component 312 to move in the second direction S2, and the first loading moving unit 321 drives the first loading component 311 to move in the first direction S1 until the cells that meet the preset requirements are spaced apart from the suction and release component 21 along the third direction S3. The selection moving component 23 drives the suction and release component 21 to move to the preset position in the third direction S3. The driving unit 251 generates an appropriate negative pressure in the hollow pipe 211 of the suction and release component 21 to absorb cells into the hollow pipe 211 of the suction and release component 21. The selection moving component 23 drives the suction and release component 21 to move in the direction away from the loading component 31. The second loading and moving unit 322 drives the second loading member 312 to move in the second direction S2, and the first loading and moving unit 321 drives the first loading member 311 to move in the first direction S1 until the storage portion 3142a for recovering and culturing cells that meet the preset requirements is spaced apart from the suction and release member 21 along the third direction S3. The selection moving assembly 23 drives the suction and release member 21 to move in the third direction S3 so that there is a preset distance between the suction and release port 212 and the storage portion 3142a. The preset distance is the distance that satisfies the suction and release member 21 to release the cells that meet the preset requirements into the storage portion 3142a. The first driving unit drives the suction and release member 21 to release the cells that meet the preset requirements sucked into the hollow tube 211 into the storage portion 3142a. The selection moving assembly 23 drives the suction and release member 21 to move in a direction away from the loading assembly 31. The above process is repeated to aspirate and release different cells that meet the preset requirements into different storage units 3142a until all cells that meet the preset requirements in the sample to be sorted are aspirated and released. The sample to be sorted is recovered and stored, and the storage unit 314 is transferred to an incubator for further incubation.

[0244] Clean the suction and placement member 21 for the second time. Specifically, first take the second holding member 2552, add an appropriate amount of the second cleaning liquid 2542, place the second holding member 2552 with the second cleaning liquid 2542 on the first carrier 311, so that the second holding member 2552 and the suction and placement member 21 are spaced apart along the third direction S3, select the driving member 231 to drive the suction and placement member 21 to move into the second cleaning liquid 2542, the driving unit 251 drives the suction and placement member 21 to absorb the second cleaning liquid 2542 into the hollow pipe 211 of the suction and placement member 21 for cleaning, and then drive the suction and placement member 21 to release the second cleaning liquid 2542 into the second holding member 2552, repeat several times, and complete the first step of cleaning for a preset number of times. Then, the first holding member 2551 is taken, and an appropriate amount of the first cleaning liquid 2541 is added. The first holding member 2551 with the first cleaning liquid 2541 is placed on the first carrier 311 so that the first holding member 2551 and the suction and placement member 21 are spaced apart along the third direction S3. Under the control of the control mechanism 60, the selection driving member 231 drives the suction and placement port 212 of the suction and placement member 21 to move into the first cleaning liquid 2541. The driving unit 251 drives the suction and placement member 21 to absorb the first cleaning liquid 2541 into the hollow pipe 211 of the suction and placement member 21 for cleaning. The suction and placement member 21 is then driven to release the first cleaning liquid 2541 into the first holding member 2551. This is repeated several times to complete the second step of cleaning for a preset number of times.

[0245] Furthermore, the operating method of the cell sorting device 100 further includes:

[0246] Obtain the bright field image information of the cell to be sorted after absorbing. Specifically, before the suction and discharge part 21 is cleaned for the second time, after absorbing and reclaiming the cell that meets the preset requirements, under the control of the control mechanism 60, open the imaging luminous source 411, the first loading mobile unit 321 and the second loading mobile unit 322 drive the cell to be sorted that needs to be observed in the part to be sorted and the second luminous source and object lens 421 are arranged along the third direction S3, the object lens moving unit 423 drives the object lens 421 to move until the image information of the cell to be sorted of required observation is clearly presented on the display mechanism 50, records the bright field image information of the cell to be sorted. The first loading mobile unit 321 and the second loading mobile unit 322 drive the part to be sorted to move to the preset area, change the cell to be sorted that needs to be observed. Repeat the above process until the bright field image information of the cell to be sorted in different areas all obtains and records. Contrast the bright field image information of the cell to be sorted before and after absorbing, draw the acquisition situation of the cell that meets the preset requirements.

[0247] The cell sorting device 100 selectively absorbs and releases cells that meet the preset requirements from the items to be sorted into the corresponding storage portion 3142a of the storage unit 314. This process is automatically completed under the control of the control mechanism 60, thereby improving the efficiency of cell sorting, avoiding damage to the aspiration and release component 21 and cells that meet the preset requirements due to manual operation, ensuring the biological activity of cells that meet the preset requirements, and realizing high-throughput cell sorting.

[0248] The bio-culture chip 200 makes full use of microscopy or nanotechnology to achieve precise control of cell capture, fixation, and culture on the chip, and through miniaturized chemical analysis methods, it achieves research goals such as high-throughput, multi-parameter, in-situ signal detection of cell samples and physical and chemical analysis of cell components.

[0249] The biological culture chip 200 is introduced in detail below.

[0250] Figures 30 to 32 A schematic diagram of a biological culture chip in some embodiments of the present application is shown.

[0251] In some embodiments, see Figures 30 to 32 The bio-culture chip 200 includes a matrix 201 and a cavity 202. The matrix 201 is formed of a matrix material. The cavity 202 is formed on the surface of the matrix 201 and is open to the surface of the matrix 201. The cavity 202 defines a bio-culture space for culturing cellular biomaterials. The structure and size of the cavity 202 are not particularly limited, as long as they can provide a space for culturing or growing single cells. The cavity 202 can have a rectangular parallelepiped, a cube, a cylinder, or other structures.

[0252] In some embodiments, the matrix material is a microporous soft matrix with good biocompatibility, cell culture medium compatibility, and mechanical tension, which can more efficiently capture and culture cells (including single cells and multi-cells aggregated in a controlled number). The soft matrix is ​​easy to be affinity and fused with different extracellular matrix environments, providing cells with a uniform and fused three-dimensional culture space, thereby creating a microenvironment for the cells in the two-dimensional array that is conducive to survival, proliferation, migration, protein expression and secretion, stem cell differentiation, etc., as well as conducive to the detection of biological functions of array cells. It should be noted that the specific type of matrix material that can be used is not particularly limited, and hard matrix materials such as glass, silicon wafers, plastics, etc. are also within the scope of protection of this application.

[0253] In some embodiments, see Figure 32, the opening diameter of the accommodating cavity 202 is smaller than the bottom diameter of the accommodating cavity 202. In this way, single cells can be easily captured for culture without the occurrence of cell escape during the culture process. Specifically, the opening diameter of the accommodating cavity 202 is at most 80% of the bottom diameter of the accommodating cavity 202, preferably 50%. The term "diameter" used is defined as the diameter of the largest area circle that the opening or bottom can accommodate. The term "depth" of the accommodating cavity 202 used refers to the shortest distance between the opening cross-section and the bottom cross-section of the accommodating cavity 202. In a feasible embodiment, the opening diameter of the accommodating cavity 202 is 8-25 microns and the depth is 15-35 microns. It should be noted that different opening diameters can be set for different cells. For example, if the biomaterial is B cells, the opening diameter is 8-12 microns; if the biomaterial is hybridoma cells or tumor cells, the opening diameter is 15-25 microns.

[0254] In some embodiments, the biological culture chip 200 may include multiple cavities 202, and the cavities 202 are discretely distributed. The cavities 202 form a predetermined pattern, and the distance between two adjacent cavities 202 is 10-100 microns. This allows for batch processing of multiple single cells. When the distance between the cavities 202 is 10-100 microns, the cells cultured in the cavities 202 will not interfere with each other, thereby improving batch processing uniformity, improving the accuracy and efficiency of cell biological function detection, and facilitating high-throughput collection and analysis of cell signaling data. The cavities 202 on the biological culture chip 200 can form an array, for example, with tens of thousands of cavities 202, or even hundreds of thousands or more cavities 202 on a single chip 200.

[0255] In some embodiments, positioning marks may be further provided on the bio-culture chip 200, and the positioning marks are formed on the matrix 201. Thus, the multiple cavities 202 can be positioned using these positioning marks. The form of the positioning marks is not particularly limited. According to the embodiments of the present application, positioning marks made of fluorescent materials can be provided to enable automatic and precise positioning, or coordinate lines can be set to position each cavity 202. When cells meeting the preset requirements are observed, each cavity 202 containing cells meeting the preset requirements can be quickly located and recorded. This further facilitates the subsequent separation of cells or other components meeting the preset requirements in the cavities 202.

[0256] In some embodiments, the method for using the biological culture chip 200 is as follows:

[0257] Place the chip 200 in the chip holder 71. Because the biological culture chip 200 needs to be stored in a low-temperature environment, such as an environment of 0-5°C. Therefore, before use, the biological culture chip 200 needs to be left at room temperature for a period of time to allow the temperature to return to room temperature. Because the biological culture chip 200 is thin, soft and brittle, it is usually placed in the chip holder 71. After the outside of the chip holder 71 containing the biological culture chip 200 is disinfected, it is transferred to a sterile environment, the chip preservation liquid is removed with a pipette, and it is washed several times with sterile 0.01M PBS buffer. It should be noted that do not touch the biological culture chip 200 during the cleaning process.

[0258] Add cells. Add the pre-prepared cells to be sorted to the chip holder 71 containing the bio-culture chip 200. Preferably, to ensure optimal single-cell array efficiency, the ratio of cell number to chip throughput is between 5:1 and 10:1, and the cell suspension volume is 1.5 ml. If the chip throughput is 100,000, a cell suspension containing 500,000 to 1,000,000 cells is used for arraying. Allow the cells to stand in a cell culture incubator for a predetermined time, such as 15 minutes, to form an array on the bio-culture chip 200. The holding chamber 202 of the bio-culture chip 200 is configured as an array.

[0259] First separation: Use a pipette to recover the arrayed cell suspension and then wash it multiple times with 0.01M PBS buffer to remove any remaining cells. The chip holder 71 can be tilted appropriately for washing. Gentle handling is required throughout the entire process to separate any cells that have not formed an array. After washing, observe the array on the cell arrayed chip 200 under a microscope.

[0260] Prepare screening medium. Prepare complete cell culture medium. Add the desired fluorescent dyes to the complete cell culture medium at the desired dilution factor. Vortex at low speed to mix thoroughly. If needed, add markers, such as pre-labeled fluorescent dye antigens, to the thoroughly mixed complete culture medium at a specific dilution ratio. Vortex at low speed to mix thoroughly. This will produce screening medium.

[0261] Add screening culture medium. Add pre-configured screening culture medium to the chip 200 behind the cell array, and then place the chip 200 behind the cell array in a cell incubator for incubation. The substances in the screening culture medium are specifically bound to the secretory substances of the cells to be sorted after the array is completed. The incubation time is set according to different projects, such as 3-8 hours for cell line development projects and no less than 8 hours for antibody discovery projects. In antibody discovery projects, antigens pre-labeled with fluorescent dyes are added to the screening culture medium for specific binding to the antibodies secreted by the cells, and the antigens pre-labeled with fluorescent dyes are used as markers. In cell line development projects, second antibodies pre-labeled with fluorescent dyes are added to the screening culture medium for specific binding to the antibodies secreted by the cells, and the second antibodies pre-labeled with fluorescent dyes are used as markers.

[0262] Second separation: After the incubation is completed, the screening culture medium is recovered and the chip is washed 3-5 times with 0.01M PBS buffer until all suspended cells are substantially removed, and the cells to be sorted after being cultured with fluorescent dye are obtained in the biological culture chip 200 .

[0263] Figure 33 FIG2 shows a schematic diagram of a chip fixing assembly in some embodiments of the present application. Figure 34 A schematic diagram of a pressure member in some embodiments of the present application is shown.

[0264] In some embodiments, see Figure 19 and Figure 33 Because the biological culture chip 200 is thin, soft, and brittle, it is difficult to directly fix it in the cell sorting apparatus 100. Therefore, the cell sorting apparatus 100 further includes a chip fixing assembly 70. The chip fixing assembly 70 can be placed on the loading mechanism 30. The chip fixing assembly 70 includes a chip holder 71, which is used to hold the biological culture chip 200. The biological culture chip 200 is first fixed in the chip holder 71, and then the chip holder 71 is placed on the loading assembly 31 of the cell sorting apparatus 100.

[0265] In some embodiments, see Figure 33The chip fixing assembly 70 is used to fix the biological culture chip 200 to the chip holder 71. The chip fixing assembly 70 also includes a pressure member 72 supported by the chip holder 71. The pressure member 72 can apply pressure to the biological culture chip 200 along the third direction S3, ensuring that the biological culture chip 200 is stably placed in the chip holder 71. The pressure member 72 is generally cylindrical with two open ends. It applies pressure to the corners of the biological culture chip 200 to ensure that the biological culture chip 200 is stably placed in the chip holder 71. Applying pressure to the corners of the biological culture chip 200 can increase the number of effective cavities 202 in the biological culture chip 200, thereby increasing the number of cells to be sorted. It is understood that the pressure member 72 is not limited to the position of the pressure member 72 as long as the biological culture chip 200 is fixed. For example, the pressure member 72 can apply pressure to the edge of the biological culture chip 200. Furthermore, to better fix the organism culture chip 200 in the chip container 71223 , the corners of the organism culture chip 200 abut against the inner wall of the chip container 71 .

[0266] To facilitate the placement and removal of the pressure member 72 and the biological culture chip 200, refer to Figure 34 The pressure member 72 includes a pressure portion 721 and a take-up portion 722. Figure 33 The pressure-applying portion 721 is accommodated in the chip holder 71 and is used to apply pressure to the biological culture chip 200. The shape and size of the pressure-applying portion 721 match those of the chip holder 71. The pick-up portion 722 is used to pick up the pressure-applying portion 722 and is used for placement and removal of the pressure-applying portion 722. The end of the pressure-applying portion 721 away from the biological culture chip 200 extends circumferentially to form the pick-up portion 722. The pick-up portion 722 protrudes from the chip holder 71 along the circumference of the chip holder 71, that is, at least a portion of the pick-up portion 722 is larger than the chip holder 71, facilitating removal of the pressure-applying portion 722.

[0267] In some embodiments, the pressure member 72 can apply pressure to the biological culture chip 200 through the pressure member 72 body. Specifically, the pressure member 72 is made of a metal material that is heavier than the biological culture chip 200, such as copper or aluminum. The pressure member 72 applies pressure to the biological culture chip 200 through its gravity. The weight of the pressure member 72 can be set based on actual needs and is not limited herein. The material of the pressure member 72 should ensure that it will not contaminate the biological chip, and the pressure member 72 must be disinfected before and after use.

[0268] In some embodiments, the pressure member 72 can apply pressure to the biological culture chip 200 by transmitting external pressure. Specifically, the pressure member 72 is made of a lightweight material and is provided with a snap-fitting portion. The chip holder 71 is provided with a snap-fitting portion. The snap-fitting portion and the snap-fitting portion engage to connect the pressure member 72 to the chip holder 71. The snap-fitting portion generates pressure on the biological culture chip 200, thereby securing the biological culture chip 200 in the chip holder 71. It is understood that the pressure member 72's pressure application method is not limited as long as it can secure the biological culture chip 200 in the chip holder 71.

[0269] In some embodiments, to ensure normal use of the biological culture chip 200 in the cell sorting apparatus 100, the cell sorting apparatus 100 further includes a fixing mechanism comprising a chip fixing assembly 70 and a loading and limiting assembly 33. The chip fixing assembly 70 is used to fix the biological culture chip 200 in the chip holder 71, and the loading and limiting assembly 33 is used to fix the chip holder 71 to the loading mechanism 30. The use of the biological culture chip 200 improves the sorting efficiency and accuracy of the cell sorting apparatus 100.

[0270] In some embodiments, when the biological culture chip 200 that has undergone cell-specific screening is used as a to-be-sorted part, the biological culture chip 200 is first fixed, the metal pressure member 72 is disinfected and sterilized, and the metal pressure member 72 is placed in the chip holder 71, so that the pre-prepared biological culture chip 200 with cells to be sorted is fixed in the chip holder 71. The chip holder 71 is then secured and placed into the receiving portion 3111 of the first carrier 311. The receiving portion 3111 is formed with a through hole and a retaining wall surrounding the through hole. An elastic member 331 is disposed on the outer surface of the chip holder 71. The elastic member 331 on one side of the receiving portion 3111 is pulled to the other side of the receiving portion 3111, biasing the elastic member 331 against the outer surface of the chip holder 71. The outer surface of the chip holder 71 is biased and retained against the retaining wall, thereby securing the chip holder 71 to the first carrier 311 and the biological culture chip 200 to the cell sorting apparatus 100. The above-described method for operating the cell sorting apparatus 100 is then repeated to achieve cell sorting.

[0271] In one feasible embodiment, the placement and limiting assembly further includes a limiting member 333, such as a silicone sleeve. The chip container 71 is placed in the limiting member 333, and then the limiting member 333 is placed in the placement portion 3111. The elastic member 331 biases against the outer surface of the limiting member 333 to secure the chip container 71 on the first placement portion 311. It is understood that the limiting member 333 can also be placed in the placement portion 3111 first, and then the chip container 71 is placed in the limiting member 333.

[0272] During aspiration, the loading and moving assembly 32 drives the receiving chamber 202 of the biological culture chip 200, containing cells meeting the preset requirements, to move until the receiving chamber 202 and the suction and placement member 21 are spaced apart along the third direction S3. The selecting and moving assembly 23 drives the suction and placement member 21 to move along the third direction S3 until a preset distance is established between the suction and placement opening 212 and the receiving chamber 202. This preset distance enables the driving unit 251 to drive the suction and placement member 21 to aspirate the cells meeting the preset requirements into the hollow conduit 211 of the suction and placement member 21. Preferably, the cells meeting the preset requirements are single cells disposed in the receiving chamber 202 of the biological culture chip 200.

[0273] The technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0274] The above-described embodiments merely represent several implementation methods of the present application. While the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent application. It should be noted that a person of ordinary skill in the art may make various modifications and improvements without departing from the spirit of the present application, and these modifications and improvements fall within the scope of protection of the present application. Therefore, the scope of protection of the present patent application shall be determined by the appended claims.

Claims

1. A cell sorting device based on a biological culture chip, characterized in that: The biological culture chip is formed with a receiving cavity for receiving cells to be sorted, wherein the receiving cavity is provided in a plurality and is discretely distributed. The cell sorting device includes: main body; a loading mechanism, the loading mechanism being configured to be mounted on the main body, the loading mechanism comprising a first loading member for loading the biological culture chip, the first loading member being movable relative to the main body so that the biological culture chip moves relative to the main body to a preset area; a chip fixing assembly, the chip fixing assembly being capable of being placed on the loading mechanism, the chip fixing assembly comprising a chip receiving member for receiving the biological culture chip; An imaging mechanism, for acquiring image information of the cells to be sorted in the biological culture chip, wherein the imaging mechanism is mounted on the main body and configured to image the cells to be sorted in the preset area; A display mechanism, configured to display the image information of the cells to be sorted acquired by the imaging mechanism; and a selection mechanism for sucking cells meeting preset requirements from the biological culture chip, the selection mechanism being configured to be mounted on the main body, the selection mechanism comprising a suction and release member movable relative to the main body, the suction and release member being configured to suck and release the cells meeting the preset requirements; The loading mechanism includes a loading assembly and a loading moving assembly. The loading assembly is provided with a first loading member for loading the chip container. The first loading member is mounted on the loading moving assembly. The loading moving assembly can drive the loading assembly to move so that the biological culture chip is placed between the selection mechanism and the imaging mechanism. The selection mechanism further includes a mounting assembly, a selection movement assembly, and a recovery assembly mounted on the main body. The mounting assembly is used to mount the suction and placement member. The selection movement assembly can drive the suction and placement member to move so that the suction and placement member approaches or moves away from the carrier mechanism. The recovery assembly is connected to the suction and placement member and can enable the suction and placement member to absorb the cells that meet the preset requirements. The selection mechanism further includes a selection lighting unit, which is connected to the mounting assembly and located at an end of the suction and placement member away from the carrier mechanism. The light emitted by the selection lighting unit can pass through the suction and placement member.

2. The cell sorting device according to claim 1, characterized in that The carrying mechanism further includes a carrying limiting assembly, which is disposed on the carrying assembly and can fix the chip receiving member to the carrying assembly.

3. The cell sorting device according to claim 2, characterized in that The carrying component includes a storage unit placed on the carrying movable component, and the storage unit is used to recover the cells that meet the preset requirements. The imaging mechanism includes an objective lens, and the objective lens is configured to image the cells to be sorted in the preset area. The carrying movable component can drive the storage unit to move so that the storage unit is placed between the suction and release component and the objective lens.

4. The cell sorting device according to claim 1, characterized in that The imaging mechanism includes an imaging lighting unit, an optical path component, a detection component and a fluorescence component. The imaging lighting unit is connected to the selection mechanism and is used to provide illumination for the biological culture chip and the optical path component. The optical path component is used to image the cells to be sorted and transmit the image information of the cells to be sorted to the detection component. The fluorescence component includes an excitation light source capable of causing the cells to be sorted to emit fluorescence. The detection component is connected to the display mechanism and can obtain bright field image information and fluorescence image information of the cells to be sorted obtained by the optical path component and transmit the obtained bright field image information and fluorescence image information of the cells to be sorted to the display mechanism.

5. The cell sorting device according to claim 1, characterized in that The chip fixing assembly includes a pressure member, which can apply pressure to the biological culture chip to fix the biological culture chip to the chip receiving member.

6. The cell sorting device according to claim 5, characterized in that The pressure member includes a pressure portion and a pick-up portion. The pressure portion is accommodated in the chip container and can apply pressure to the biological culture chip. The pick-up portion is configured to extend circumferentially along one end of the pressure portion away from the biological culture chip. The pick-up portion protrudes from the chip container.

7. A cell sorting method, characterized in that: The cell sorting device according to any one of claims 1 to 6, wherein the method comprises: Placing cells to be sorted in the bio-culture chip; Placing the biological culture chip on the loading mechanism; Acquiring image information of the cells to be sorted; Select cells that meet preset requirements based on image information of the cells to be sorted; and The cells meeting the preset requirements in the biological culture chip are aspirated.

Images