A device for preparing enzyme-catalyzed cartilage repair hydrogel

Abstract

The present invention discloses an enzyme-catalyzed cartilage repair hydrogel preparation device in the field of biomaterial preparation technology, including a shell, a storage chamber, a reaction chamber and a heating chamber are arranged inside the shell, a sampling assembly is installed in the storage chamber, a stirring assembly and a monitoring assembly are installed in the reaction chamber, and a filter assembly is installed between the reaction chamber and the heating chamber; the filter assembly includes a mounting shell, a connecting plate, a connecting rod and a fan blade, the fan blades all penetrate the side wall of the mounting shell, the fan blades penetrate one end of the mounting shell and are fixedly connected to the connecting plate, a connecting rod is hinged between adjacent connecting plates, a first power member is fixedly connected in the mounting chamber, the output shaft of the first power member is fixedly connected to one end of the fan blade, a slide groove is arranged on the top of the mounting shell, and a filter plate is slidably matched inside the slide groove. The present invention improves the preparation efficiency and quality of hydrogel and reduces the preparation cost through optimization of multiple aspects such as integrated design, precise material control and intelligent filtration.

Description

Technical Field

[0001] The invention belongs to the technical field of biomaterial preparation, and in particular is a device for preparing enzyme-catalyzed cartilage repair hydrogel. Background Art

[0002] Cartilage tissue is an important component of the human skeletal system and plays a vital role in the normal morphology and function of joints. However, due to the extremely low content of blood vessels and lymphatic tissue in cartilage tissue, its regeneration capacity is extremely limited. When cartilage tissue is damaged or missing, it is often difficult to recover through its own repair mechanism, which leads to the occurrence of various joint diseases and movement disorders. Therefore, exploring effective cartilage repair methods has always been a research hotspot in the field of biomedical engineering.

[0003] As a potential cartilage filling material, hydrogel has attracted much attention due to its unique properties. Hydrogel has the characteristics of easy molding, three-dimensional hydrophilic network and ideal biocompatibility, which makes it an ideal material for cartilage repair. Injectable hydrogels, in particular, have significant advantages in cartilage tissue engineering due to their unique sol-gel transition characteristics and adjustable mechanical properties.

[0004] However, the existing enzyme-catalyzed cartilage repair hydrogel preparation technology still has some defects:

[0005] 1. Instability of enzyme-catalyzed reactions: The rate and efficiency of enzyme-catalyzed reactions are affected by many factors, such as temperature, pH value, substrate concentration, enzyme activity and stability, etc. Fluctuations in these factors may lead to instability in the preparation process, thereby affecting the quality and performance of the hydrogel.

[0006] 2. Complexity of the preparation process: In the process of preparing hydrogels for enzyme-catalyzed cartilage repair, the steps of enzyme-catalyzed reaction, hydrogel formation and product drying are usually carried out in steps. The connection and switching between these steps usually require independent use of professional equipment and judgment based on experience, resulting in high costs for preparing hydrogels for enzyme-catalyzed cartilage repair, and the quality and performance of the hydrogels are difficult to guarantee.

[0007] Therefore, the present invention proposes an enzyme-catalyzed cartilage repair hydrogel preparation device to solve the above problems. Summary of the invention

[0008] The present invention proposes an enzyme-catalyzed cartilage repair hydrogel preparation device, which improves the preparation efficiency and quality of the hydrogel and reduces the preparation cost through optimization of multiple aspects such as integrated design, precise material control and intelligent filtration.

[0009] In order to achieve the above-mentioned object, the technical scheme of the present invention is as follows: an enzyme-catalyzed cartilage repair hydrogel preparation device, comprising a shell, wherein a plurality of storage chambers, a reaction chamber and a heating chamber are sequentially arranged inside the shell from top to bottom, a sampling assembly is installed in each storage chamber, the storage chamber is connected with the reaction chamber through the sampling assembly, a stirring assembly and a monitoring assembly are installed in the reaction chamber, a filtering assembly is installed between the reaction chamber and the heating chamber, and the reaction chamber and the heating chamber are connected through the filtering assembly;

[0010] The filter assembly includes a mounting shell, a plurality of connecting plates, a plurality of connecting rods and a plurality of fan blades. The mounting shell is fixedly connected to the inner wall of the outer shell, and a mounting chamber is provided between the mounting shell and the inner wall of the outer shell. The fan blades all penetrate the side wall of the mounting shell and are rotatably matched with the side wall of the mounting shell. The fan blades are distributed along the circumference of the inner wall of the mounting shell. One end of the fan blades penetrating the mounting shell is fixedly connected to the connecting plate. Connecting rods are hinged between adjacent connecting plates. A first power component is fixedly connected in the mounting chamber. The output shaft of the first power component is fixedly connected to one end of one of the fan blades penetrating the mounting shell. A slide groove is provided on the top of the mounting shell, and a filter plate is slidably matched inside the slide groove.

[0011] The following beneficial effects are achieved by adopting the above scheme:

[0012] 1. The device of the present invention is provided with a storage chamber, a reaction chamber and a heating chamber from top to bottom. This layout enables the material feeding, reaction and drying processes to be carried out sequentially, and the steps of enzyme catalysis reaction, hydrogel formation and product drying are seamlessly connected with monitoring of each link, thereby reducing material transfer, improving efficiency, ensuring the quality and performance of the hydrogel, and reducing pollution.

[0013] 2. The materials in the storage chamber are put into the reaction chamber in a preset proportion and sequence through the injection component, which ensures the accuracy of material delivery and the uniformity of mixing, and is conducive to improving the quality and performance of the hydrogel.

[0014] 3. The stirring component installed in the reaction chamber can ensure that the materials are evenly mixed during the reaction process, improving the reaction efficiency and the uniformity of the product. The monitoring component can monitor the reaction process in real time, providing a basis for the control of the reaction conditions. At the same time, the controller can automatically adjust the reaction conditions according to the monitoring data to ensure the stability and controllability of the reaction process.

[0015] 4. The design of the fan blades allows the first power member to quickly change state when needed, thereby controlling the lifting and lowering of the filter plate to achieve isolation or connection between the reaction chamber and the heating chamber. The combination of the fan blades, connecting plates and connecting rods enables all the fan blades to move synchronously, enhancing the stability and reliability of the operation. The design of the chute provides a stable sliding track for the filter plate, ensuring its stability and accuracy during the lifting process.

[0016] 5. The entire preparation process can be automatically controlled by the controller, reducing the workload of manual operation and improving production efficiency. The entire preparation process is carried out in a closed device, reducing the emission of harmful substances, and the recycling and reuse design also reduces energy consumption.

[0017] Furthermore, the side walls of the storage chamber are provided with a feed port and a cover body for closing the feed port, the bottom of the storage chamber is provided with a discharge port, the sampling assembly includes a plurality of second power parts, the second power part is fixedly connected to the inner side wall of the shell, a cam is fixedly connected to the output shaft of the second power part, the diameters of the cams are not equal, a circular hole is opened on the top wall of the storage chamber, a push rod is inserted in the circular hole, a mounting plate is fixedly connected to the top end of the push rod, a roller is installed on the mounting plate away from the push rod, a spring is sleeved on the push rod, one end of the spring is fixedly connected to the mounting plate, and the other end of the spring is fixedly connected to the top wall of the storage chamber, the push rod extends into the storage chamber and is fixedly connected to a push plate, the push plate is slidably matched with the inner wall of the storage chamber, a strain gauge is installed on the inner wall of the storage chamber, an electromagnetic valve is installed at the discharge port, the electromagnetic valve signal is connected to a controller, the controller is connected to the strain gauge and the second power part signal, and the controller controls the opening and closing of the electromagnetic valve and the second power part.

[0018] Beneficial effects: The rotation of the cam is driven by the second power member, and the contact and disengagement between the raised part of the cam and the roller is used to drive the push rod and the push plate to move up and down, thereby achieving accurate feeding of materials into the storage chamber. Since the diameters of the cams are not equal, the movement stroke of the push rod can be adjusted as needed to control the amount of material fed, thereby ensuring the accuracy and consistency of the reaction. The entire feeding process is automatically controlled through the cooperation of the controller, strain gauge and the second power member, without the need for manual intervention, which greatly improves the degree of automation and operating efficiency of the preparation process. The strain gauge can detect the movement state of the push plate in real time and transmit the data to the controller. The controller controls the opening and closing of the solenoid valve and the operation of the second power member according to the signal of the strain gauge, realizing real-time feedback and adjustment of the feeding process, further ensuring the accuracy and reliability of the feeding.

[0019] Furthermore, a condensation plate is fixedly connected to the top of the reaction chamber, a collection chamber is provided on the top of the reaction chamber, and the collection chamber is provided with a collection port, which is located below one side of the condensation plate.

[0020] Beneficial effects: The water vapor generated during the reaction will be quickly condensed into liquid water when it contacts the condensation plate, and will flow into the collection chamber through the preset collection port. This design effectively recovers water vapor, avoids the waste of water resources, and also helps to maintain the stability of the environment in the reaction chamber. By recovering water vapor by condensation, it is possible to avoid the accumulation of water vapor in the reaction chamber, thereby reducing the impact of water vapor on the reaction process and ensuring the quality and preparation effect of the hydrogel. The water recovered by condensation can be further utilized, such as being used in a water bath chamber to replenish the water volume in the water bath chamber and ensure the temperature conditions during the catalytic process, thereby improving energy utilization efficiency.

[0021] Furthermore, recovery chambers are provided on both sides of the heating chamber, the recovery chamber is connected with the heating chamber, and a one-way valve is installed at the connection point.

[0022] Beneficial effects: The water used to wash the hydrogel is recovered through the recovery chamber and reheated for reuse, thus realizing the recycling of water resources, greatly reducing the consumption of water resources, and also helping to reduce production costs. The temperature of the recovered and heated water can be effectively controlled, ensuring that the temperature conditions of the water meet the requirements in the subsequent preparation process, thereby helping to improve the quality and preparation efficiency of the hydrogel. Using a heating component to heat the water in the recovery chamber not only avoids the energy consumption of reheating when using new water, but also further improves energy efficiency by effectively utilizing the thermal energy of the recycled water.

[0023] Furthermore, a water bath chamber is provided on the outer side of the bottom of the reaction chamber, the top of the water bath chamber is connected with the collection chamber, a water pump is installed in the recovery chamber, the output end of the water pump is connected with the bottom of the water bath chamber, and electric valves are installed at the connection points between the water bath chamber and the collection chamber and the connection points with the water pump, and the electric valves are connected with the controller signal.

[0024] Beneficial effects: The water bath chamber provides a constant temperature environment for the reaction chamber by means of a water bath, which is conducive to the stable progress of the enzyme catalytic reaction. At the same time, due to the connection design between the water bath chamber and the collection chamber and the recovery chamber, the water bath temperature can be adjusted as needed, thereby achieving precise control of the reaction temperature. The water recovered in the recovery chamber for washing the hydrogel is re-injected into the water bath chamber through a water pump, realizing the recycling of water resources. This not only reduces the consumption of water resources, but also reduces production costs. By controlling the electric valve by the controller, the water flow circulation and switching between the water bath chamber and the collection chamber and the recovery chamber can be automatically realized. This design improves the degree of automation of the preparation process, reduces manual operations, and improves production efficiency.

[0025] Furthermore, the stirring assembly includes a third power member, the third power member is fixedly connected to the inner wall of the top of the reaction chamber, the output shaft of the third power member is fixedly connected to the stirring shaft, and the stirring shaft is fixedly connected to a plurality of stirring rods.

[0026] Beneficial effects: The stirring shaft is driven to rotate by the third power member, driving the stirring rod to stir the materials in the reaction chamber. This design ensures that the materials can be fully mixed during the reaction process, avoiding the situation where the local concentration is too high or too low, thereby improving the uniformity and consistency of the reaction. Through stirring, the contact area between the materials is increased, which is conducive to the enzyme-catalyzed reaction. At the same time, stirring can also destroy bubbles and attachments on the surface of the material, promote full contact between the material and the enzyme, and further improve the reaction efficiency. During the reaction process, some materials may settle at the bottom of the reaction chamber due to density differences. The continuous operation of the stirring assembly can effectively prevent the precipitation of materials, maintain the uniform distribution of materials, and ensure the smooth progress of the reaction.

[0027] Furthermore, a cross bar is fixedly connected to the upper portion of the stirring shaft, and a scraper is fixedly connected to one end of the cross bar away from the stirring shaft.

[0028] Beneficial effects: The design of the crossbar and the scraper enables the stirring assembly to not only stir the material with the stirring shaft and the stirring rod, but also scrape the inner wall of the reaction chamber to ensure that the material does not adhere to the inner wall of the chamber, thereby improving the stirring effect and the uniformity of the material. The scraper can effectively scrape off the material residue on the inner wall of the reaction chamber, avoiding the accumulation and precipitation of the material on the inner wall of the chamber. This is not only conducive to keeping the reaction chamber clean, but also ensures the efficiency of material use and the quality of the reaction in the next preparation process. By scraping off the material residue on the inner wall of the reaction chamber, material waste and loss can be reduced, thereby optimizing the preparation process and reducing production costs.

[0029] Furthermore, the monitoring component includes a first temperature sensor, a second temperature sensor and a pH sensor, the first temperature sensor and the pH sensor are installed inside the reaction chamber, the second temperature sensor is installed inside the water bath chamber, and the first temperature sensor, the second temperature sensor and the pH sensor are all connected to the controller signal.

[0030] Beneficial effects: By real-time monitoring of the temperature inside the reaction chamber by the first temperature sensor, monitoring of the temperature inside the water bath chamber by the second temperature sensor, and monitoring of the pH value of the reaction solution by the pH sensor, the controller can obtain real-time data and make precise adjustments and controls accordingly. This ensures that the reaction process is carried out under optimal conditions and improves the quality and yield of the hydrogel. Based on the real-time monitoring data, the controller can automatically adjust parameters such as heating power and stirring speed to optimize the reaction conditions. This helps to reduce energy consumption, improve reaction efficiency, and reduce production problems caused by improper human operation. By real-time monitoring of key parameters in the reaction process, such as temperature and pH value, abnormalities can be detected in time and corresponding measures can be taken to avoid production accidents. At the same time, this also helps to improve production stability and ensure the consistency of product quality.

[0031] Furthermore, a heating component is installed on the inner wall of the bottom of the heating chamber. The heating component is connected to the controller signal. The controller controls the power of the heating component according to the real-time monitoring data of the monitoring component.

[0032] Beneficial effects: By monitoring the temperature of the reaction chamber and the water bath chamber in real time, the controller can accurately adjust the power of the heating component as needed, thereby ensuring that the reaction process is carried out under constant temperature conditions. This is especially important for enzyme-catalyzed reactions, as changes in temperature may affect the activity and reaction rate of the enzyme. Accurate temperature control helps enzyme-catalyzed reactions to proceed at the optimal temperature, thereby improving reaction efficiency. This not only reduces reaction time, but also improves the yield and quality of hydrogels. By adjusting the power of the heating component in real time, unnecessary energy waste can be avoided. When the temperature reaches the set value, the controller can reduce the power of the heating component or turn off the heating, thereby saving energy.

[0033] Furthermore, a visual window is embedded in the side wall of the reaction chamber, and the visual window is made of a transparent material. A sampling port and a cover for closing the sampling port are opened on the side wall of the reaction chamber.

[0034] Beneficial effects: Through the visual window, the operator can intuitively observe the reaction process such as the state, color, and bubble generation of the material in the reaction chamber, so as to understand the progress of the reaction in real time. When abnormal conditions occur during the reaction, such as material precipitation, abnormal color, etc., the operator can quickly find the problem through the visual window, and adjust the reaction conditions or take corresponding measures in time according to the situation to avoid the expansion of the problem or affect the product quality. Real-time observation of the reaction process helps the operator to grasp the reaction status more accurately, reduce unnecessary guesswork and waiting time, and improve operational efficiency. At the same time, observing the reaction process through the visual window can reduce the number of times the reaction chamber is directly opened for viewing, reduce operational risks, and improve safety. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 This is an axonometric diagram of an embodiment of the device for preparing the enzyme-catalyzed cartilage repair hydrogel of the present invention.

[0036] Figure 2 This is a front view of an embodiment of the device for preparing the enzyme-catalyzed cartilage repair hydrogel of the present invention.

[0037] Figure 3 for Figure 2 Cross-sectional view along the AA direction.

[0038] Figure 4 It is an axonometric diagram of the filtering component in the embodiment of the device for preparing the hydrogel for enzyme-catalyzed cartilage repair according to the present invention.

[0039] Figure 5 for Figure 3A magnified schematic diagram of center A.

[0040] Figure 6 This is an axonometric diagram of the gel cutting assembly in the embodiment of the device for preparing the hydrogel for enzyme-catalyzed cartilage repair according to the present invention.

[0041] The figure marks in the drawings of the specification include: shell 1, storage chamber 2, discharge port 201, solenoid valve 202, reaction chamber 3, condensation plate 301, sampling port 302, heating chamber 4, heating component 401, mounting shell 5, mounting chamber 501, fan blade 502, connecting plate 503, connecting rod 504, first motor 505, slide 506, filter plate 507, second motor 6, cam 601, roller 602, mounting plate 603, push rod 604, spring 605, push plate 606, strain gauge 607, collection chamber 7, collection port 701, recovery chamber 8, water pump 801, third motor 9, stirring shaft 901, stirring rod 902, cross bar 903, scraper 904, water bath chamber 10, glue tray 11, mounting frame 12, cutting knife 13. DETAILED DESCRIPTION

[0042] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0043] In the description of the present invention, it is to be understood that the terms “longitudinal”, “lateral”, “vertical”, “up”, “down”, “front”, “back”, “left”, “right”, “vertical”, “horizontal”, “top”, “bottom”, “inside” and “outside” etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0044] In the description of the present invention, unless otherwise specified and limited, it should be noted that the terms "installed", "connected" and "connected" should be understood in a broad sense. For example, it can be a mechanical connection or an electrical connection, or it can be the internal connection between two components. It can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms can be understood according to the specific circumstances.

[0045] The following is further described in detail through specific implementation methods:

[0046] Embodiment 1, basically as attached Figures 1 to 5As shown: an enzyme-catalyzed cartilage repair hydrogel preparation device, comprising a shell 1, wherein the inside of the shell 1 is provided with a plurality of storage chambers 2, a reaction chamber 3 and a heating chamber 4 from top to bottom, wherein each storage chamber 2 is provided with a sampling assembly, wherein the storage chamber 2 is connected with the reaction chamber 3 through the sampling assembly, wherein the side walls of the storage chamber 2 are provided with a feeding port and a cover body for sealing the feeding port, wherein the bottom of the storage chamber 2 is provided with a discharge port 201, wherein the sampling assembly comprises a plurality of second power members, wherein the second power members are fixedly connected to the inner wall of the shell 1 by screws, wherein a cam 601 is fixedly connected to the output shaft of the second power member by screws, wherein the diameters of the cams 601 are not equal, wherein a circular hole is provided on the top wall of the storage chamber 2, wherein a push rod 604 is inserted in the circular hole, and wherein the push rod 604 is pushed outwardly. The end is fixedly connected with a mounting plate 603 by screws, and a roller 602 is installed at one end of the mounting plate 603 away from the push rod 604. A spring 605 is sleeved on the push rod 604, and one end of the spring 605 is fixedly connected to the mounting plate 603, and the other end of the spring 605 is fixedly connected to the top wall of the storage chamber 2. The push rod 604 extends into the storage chamber 2 and is fixedly connected with a push plate 606 by screws. The push plate 606 slides with the inner wall of the storage chamber 2, and a strain gauge 607 is installed on the inner wall of the storage chamber 2. A solenoid valve 202 is installed at the discharge port 201. The signal of the solenoid valve 202 is connected to a controller, and the controller is connected to the strain gauge 607 and the second power component signal. The controller controls the opening and closing of the solenoid valve 202 and the second power component.

[0047] A stirring assembly and a monitoring assembly are installed in the reaction chamber 3. The stirring assembly includes a third power member, which is fixedly connected to the inner side wall of the top of the reaction chamber 3 by screws. The output shaft of the third power member is fixedly connected to a stirring shaft 901 by screws, and the stirring shaft 901 is fixedly connected to a plurality of stirring rods 902 by screws. A cross bar 903 is fixedly connected to the upper part of the stirring shaft 901 by screws, and a scraper 904 is fixedly connected to the end of the cross bar 903 away from the stirring shaft 901 by screws. The monitoring assembly includes a first temperature sensor, a second temperature sensor and a pH sensor. The first temperature sensor and the pH sensor are installed inside the reaction chamber 3, and the second temperature sensor is installed inside the water bath chamber 10. The first temperature sensor, the second temperature sensor and the pH sensor are all connected to the controller signal.

[0048] A filter assembly is installed between the reaction chamber 3 and the heating chamber 4, and the reaction chamber 3 and the heating chamber 4 are connected through the filter assembly. The filter assembly includes a mounting shell 5, a plurality of connecting plates 503, a plurality of connecting rods 504 and a plurality of fan blades 502. The mounting shell 5 is fixedly connected to the inner wall of the outer shell 1 by screws. A mounting chamber 501 is arranged between the mounting shell 5 and the inner wall of the outer shell 1. The fan blades 502 all penetrate the side wall of the mounting shell 5 and rotate with the side wall of the mounting shell 5. The fan blades 502 are distributed along the circumference of the inner wall of the mounting shell 5. The fan blades 502 penetrate one end of the mounting shell 5 and are fixedly connected to the connecting plate 503. Connecting rods 504 are hinged between adjacent connecting plates 503. A first power member is fixedly connected in the mounting chamber 501 by screws. The output shaft of the first power member is fixedly connected to one end of one of the fan blades 502 that penetrates the mounting shell 5. A slide groove 506 is arranged on the top of the mounting shell 5, and a filter plate 507 is slidably matched inside the slide groove 506. A visual window is embedded in the side wall of the reaction chamber 3 , and the visual window is made of a transparent material. A sampling port 302 and a cover for closing the sampling port 302 are opened on the side wall of the reaction chamber 3 .

[0049] A condensation plate 301 is fixedly connected to the top of the reaction chamber 3 by screws, and a collection chamber 7 is provided on the top of the reaction chamber 3. The collection chamber 7 is provided with a collection port 701, and the collection port 701 is located below one side of the condensation plate 301. Recovery chambers 8 are provided on both sides of the heating chamber 4. The recovery chamber 8 is connected to the heating chamber 4, and a one-way valve is installed at the connection. A water bath chamber 10 is provided on the outside of the bottom of the reaction chamber 3. The top of the water bath chamber 10 is connected to the collection chamber 7. A water pump 801 is installed in the recovery chamber 8. The output end of the water pump 801 is connected to the bottom of the water bath chamber 10. Electric valves are installed at the connection between the water bath chamber 10 and the collection chamber 7 and the water pump 801. The electric valve is connected to the controller signal.

[0050] A heating component 401 is installed on the inner wall of the bottom of the heating chamber 4. In this embodiment, the heating component 401 can adopt methods such as hot air circulation and radiation heating to ensure uniform temperature distribution in the entire heating chamber 4. The heating component 401 is connected to the controller signal, and the controller controls the power of the heating component 401 according to the real-time monitoring data of the monitoring component.

[0051] In this embodiment, the first power member, the second power member and the third power member are respectively the first motor 505, the second motor 6 and the third motor 9. According to actual conditions, pneumatic motors, hydraulic motors and electromagnetic drives can be selected as power sources.

[0052] The specific implementation process is as follows: the materials required for preparing the hydrogel are placed in different storage chambers 2 respectively, and the materials in the storage chambers 2 are put into the reaction chamber 3 through the injection assembly. That is, the second motor 6 is started by the controller, and the output shaft of the second motor 6 drives the cam 601. When the cam 601 rotates, the raised part of the cam 601 abuts against the roller 602, and the roller 602 moves downward; when the raised part of the cam 601 is separated from the roller 602, the roller 602 moves upward. Since the diameters of the cams 601 are not equal, the movement stroke of the push rod 604 can be changed according to the sizes of different cams 601, thereby adjusting the amount of material delivered.

[0053] When the protruding part of the cam 601 rotates to the bottom, the push rod 604 moves downward to drive the push plate 606, and at the same time squeezes the spring 605. When the push plate 606 slides on the inner wall of the storage chamber 2, it passes through the strain gauge 607. The strain gauge 607 transmits the detection data to the controller. After receiving the signal of the strain gauge 607, the controller starts to open the solenoid valve 202. When the push plate 606 slides in the storage chamber 2, the material in the storage chamber 2 is pushed out to the discharge port 201 into the reaction chamber 3. When the protruding part of the cam 601 rotates to the top, the spring 605 drives the push rod 604 to move upward by squeezing the spring 605 before, so that the push plate 606 moves upward. When the push plate 606 slides on the inner wall of the storage chamber 2, it passes through the strain gauge 607 again. The strain gauge 607 transmits the detection data to the controller. After receiving the signal of the strain gauge 607 again, the controller starts to close the solenoid valve 202.

[0054] When the controller receives the signal that the electromagnetic valve 202 is closed, the third motor 9 is started. The third motor 9 drives the stirring shaft 901 according to the preset time and power output. The stirring shaft 901 drives the stirring rod 902 and the scraper 904. The stirring rod 902 mixes and stirs the materials in the reaction chamber 3. During the stirring process, the scraper 904 scrapes the materials on the side wall of the reaction chamber 3 to avoid waste. When the materials are mixed evenly, the mixed materials undergo a catalytic reaction in the reaction chamber 3 until the hydrogel is completely formed. During the catalytic process, the first temperature sensor and pH sensor of the monitoring component monitor the temperature and pH value in real time.

[0055] After the hydrogel is formed, sterile water or physiological saline is poured into the reaction chamber 3 through the water inlet (not shown in the figure) on the side wall of the reaction chamber 3, and the third motor 9 is started again. The third motor 9 drives the stirring rod 902 to wash the hydrogel to remove unreacted substrates, enzymes and other impurities. The power of the third motor 9 during the washing process is lower than the power when the materials are mixed to avoid damaging the structure of the hydrogel.

[0056] After washing is completed, the third motor 9 is turned off by the controller, and the first motor 505 is started at the same time. The output shaft of the first motor 505 drives the fan blade 502, and the fan blade 502 is changed from a horizontal state to a vertical state. At the same time, the connecting plate 503 and the connecting rod 504 on the fan blade 502 drive the adjacent fan blade 502 at the same time. When all the fan blades 502 are changed from a horizontal state to a vertical state, the filter plate 507 in the chute 506 will be lifted up, and the water for washing the hydrogel will flow from the heating chamber 4 into the recovery chamber 8. The heating component 401 is started by the controller, and the heating component 401 begins to dry the hydrogel on the filter plate 507 located in the reaction chamber 3. During the drying process, as the water in the hydrogel evaporates, the generated water vapor will rise to the top of the reaction chamber. When the water vapor contacts the condensation plate 301 located at the top of the reaction chamber, it will be condensed into liquid water by the condensation plate 301 and flow into the collection chamber 7 through the preset collection port 701. At the same time, the heating component 401 can also heat the water recovered in the recovery chamber 8. This step can not only reuse the recovered water, but also ensure the temperature of the recovered water for subsequent use.

[0057] After the drying process is completed, the dried hydrogel is taken out from the sampling port 302. Subsequently, the first motor 505 is controlled by the controller to rotate in the opposite direction so that all the blades 502 that were originally in a vertical state are transformed into a horizontal state. After the blades 502 are transformed into a horizontal state, the heating chamber 4 and the reaction chamber 3 are isolated to prevent heat exchange. The condensed water collected in the collection chamber 7 is then placed in the water bath chamber 10. The low temperature of the condensed water in this process can quickly reduce the temperature of the reaction chamber 3 that has increased due to drying, so that it can quickly return to a condition suitable for the next catalytic reaction, thereby reducing the waiting time. At the same time, the water pump 801 is started by the controller. The water pump 801 starts working and pumps the heated water pump 801 in the recovery chamber 8 into the water bath chamber 10. This step not only replenishes the amount of water in the water bath chamber 10, but also further ensures the temperature conditions during the catalytic process. Subsequently, preparations for the next catalytic reaction are started. At this time, since the temperature of the reaction chamber 3 has been restored to a suitable condition and there is enough water in the water bath chamber 10, the waiting time can be shortened and the production efficiency can be improved. Specific embodiment one:

[0059] 1. Material preparation:

[0060] (1) Monomer:

[0061] Acrylic acid: As the main hydrogel-forming monomer, it is used to form polymer chains in the polymerization reaction.

[0062] Acrylamide: Another common monomer that can also participate in polymerization reactions and provide specific properties to the hydrogel.

[0063] (2) Cross-linking agent:

[0064] Methacrylate: used to react with monomers in chemical cross-linking reactions to form a three-dimensional network structure, thereby enhancing the stability and mechanical properties of the hydrogel.

[0065] (3) Bioactive substances:

[0066] Cartilage growth factor: In order to promote the proliferation and differentiation of chondrocytes and accelerate the cartilage repair process, biologically active substances such as cartilage growth factor can be added.

[0067] (4) Enzymes:

[0068] Specific enzymes: used to catalyze key reactions in the hydrogel formation process, ensuring the specific structure and properties of the hydrogel.

[0069] 2. Material adding process:

[0070] (1) Addition of monomers and crosslinking agents:

[0071] Monomers such as acrylic acid and acrylamide and cross-linking agents such as methacrylate are placed in different storage chambers 2 respectively.

[0072] The monomer and the cross-linking agent are introduced into the reaction chamber 3 through the injection assembly according to a preset ratio and sequence.

[0073] (2) Addition of biologically active substances:

[0074] At an appropriate time point, bioactive substances such as cartilage growth factor are added into the reaction chamber 3 through the injection component to ensure that they can be evenly dispersed in the hydrogel.

[0075] (3) Enzyme addition:

[0076] After the materials in the reaction chamber 3 are mixed uniformly, the enzyme is added into the reaction chamber 3 to start the catalytic reaction.

[0077] 3. Precautions during the preparation process:

[0078] Temperature control: Ensure that the temperature during the reaction is appropriate to promote the smooth progress of polymerization and cross-linking reactions.

[0079] Stirring and mixing: The stirring rod 902 and the scraper 904 are used to stir the materials in the reaction chamber 3 to ensure that the materials are mixed evenly to avoid excessively high or low concentrations in some areas.

[0080] Real-time monitoring: The first temperature sensor and the pH sensor are used to monitor the temperature and pH value in real time during the reaction to ensure that the reaction conditions are appropriate.

[0081] 4. Drying and post-treatment of hydrogel:

[0082] Drying treatment: The hydrogel on the filter plate 507 is dried by the heating component 401 to remove excess water.

[0083] Embodiment 2 is different from the above embodiment in that, as shown in the attached Figure 6 As shown: a glue tray 11 is embedded on the top of the shell 1, and a glue cutting assembly is installed on one side of the glue tray 11. The glue cutting assembly includes a mounting frame 12, and the mounting frame 12 is hinged to one side of the glue tray 11. Slide rods are embedded on the inner side walls of the mounting frame 12, and a cutting knife 13 (in this embodiment, the cutting knife 13 adopts a linear cutting knife 13) is slidably matched on the sliding rod. The cutting knife 13 is staggered in the horizontal direction and the vertical direction, and a top cover is fixedly connected to the top of the mounting frame 12.

[0084] The specific implementation process is as follows: Place the hydrogel taken out from the sampling port 302 in the gel tray 11, and ensure that the hydrogel sample is stably placed on the gel tray 11 for subsequent cutting operations. According to the required size of the hydrogel, adjust the cutting range to the appropriate size by sliding the cutting knife 13 in the horizontal and vertical directions. This process requires careful operation to ensure the accuracy and precision of the cutting range. When the cutting range is adjusted, press the mounting frame 12 downward. During this process, the cutting knife 13 will move according to the pre-set path, thereby accurately cutting the hydrogel sample to the required size. After the cutting is completed, the cut hydrogel sample can be easily taken out and used directly.

[0085] Due to the presence of the top cover on the top of the mounting frame 12, on the one hand, it can ensure that the hydrogel sample will not be contaminated during the cutting process, and on the other hand, the cutting knife 13 is also effectively protected. The above is only an embodiment of the present invention, and the common sense such as the known specific structure and / or characteristics in the scheme is not described in detail here. It should be pointed out that for those skilled in the art, several deformations and improvements can be made without departing from the structure of the present invention, which should also be regarded as the scope of protection of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. An enzyme-catalyzed cartilage repair hydrogel preparation device, characterized in that: The invention comprises a shell, wherein a plurality of storage chambers, a reaction chamber and a heating chamber are sequentially arranged inside the shell from top to bottom, a sampling assembly is installed in each storage chamber, the storage chamber is connected with the reaction chamber through the sampling assembly, a stirring assembly and a monitoring assembly are installed in the reaction chamber, a filtering assembly is installed between the reaction chamber and the heating chamber, the reaction chamber and the heating chamber are connected through the filtering assembly, and a water bath chamber is arranged outside the bottom of the reaction chamber; The filter assembly includes a mounting shell, a plurality of connecting plates, a plurality of connecting rods and a plurality of fan blades. The mounting shell is fixedly connected to the inner wall of the outer shell, and a mounting chamber is provided between the mounting shell and the inner wall of the outer shell. The fan blades all penetrate the side wall of the mounting shell and are rotatably matched with the side wall of the mounting shell. The fan blades are distributed along the circumference of the inner wall of the mounting shell. One end of the fan blades penetrating the mounting shell is fixedly connected to the connecting plate. Connecting rods are hinged between adjacent connecting plates. A first power member is fixedly connected in the mounting chamber. The output shaft of the first power member is fixedly connected to one end of one of the fan blades penetrating the mounting shell. A slide groove is provided on the top of the mounting shell, and a filter plate is slidably matched inside the slide groove. The side walls of the material storage chamber are each provided with a feed port and a cover body for closing the feed port, a material storage chamber bottom is provided with a material discharge port, the sampling assembly includes a plurality of second power members, the second power member is fixedly connected to the inner side wall of the shell, a cam is fixedly connected to the output shaft of the second power member, the diameters of the cams are not equal, a circular hole is opened on the top wall of the material storage chamber, a push rod is inserted in the circular hole, a mounting plate is fixedly connected to the top end of the push rod, a roller is installed on the mounting plate away from the push rod, a spring is sleeved on the push rod, one end of the spring is fixedly connected to the mounting plate, and the other end of the spring is fixedly connected to the top wall of the material storage chamber, one end of the push rod extending into the material storage chamber is fixedly connected to a push plate, the push plate is slidably matched with the inner side wall of the material storage chamber, a strain gauge is installed on the inner side wall of the material storage chamber, an electromagnetic valve is installed at the material discharge port, the electromagnetic valve signal is connected to a controller, the controller is connected to the strain gauge and the second power member signal, and the controller controls the opening and closing of the electromagnetic valve and the second power member; A condensation plate is fixedly connected to the top of the reaction chamber, a collection chamber is provided on the top of the reaction chamber, and a collection port is provided in the collection chamber, which is located below one side of the condensation plate; The stirring assembly includes a third power member, which is fixedly connected to the inner wall of the top of the reaction chamber, the output shaft of the third power member is fixedly connected to the stirring shaft, and the stirring shaft is fixedly connected to a plurality of stirring rods; the upper part of the stirring shaft is fixedly connected to a cross bar, and the end of the cross bar away from the stirring shaft is fixedly connected to a scraper; The monitoring component includes a first temperature sensor, a second temperature sensor and a pH sensor, the first temperature sensor and the pH sensor are installed inside the reaction chamber, the second temperature sensor is installed inside the water bath chamber, and the first temperature sensor, the second temperature sensor and the pH sensor are all connected to the controller signal; A heating component is installed on the inner wall of the bottom of the heating chamber. The heating component is connected to the controller signal. The controller controls the power of the heating component according to the real-time monitoring data of the monitoring component.

2. The device for preparing enzyme-catalyzed cartilage repair hydrogel according to claim 1, characterized in that: Recovery chambers are arranged on both sides of the heating chamber. The recovery chamber is communicated with the heating chamber, and a one-way valve is installed at the communication point.

3. The device for preparing enzyme-catalyzed cartilage repair hydrogel according to claim 1, characterized in that: The top of the water bath chamber is connected with the collection chamber, a water pump is installed in the recovery chamber, the output end of the water pump is connected with the bottom of the water bath chamber, electric valves are installed at the connection points between the water bath chamber and the collection chamber and the water pump, and the electric valves are connected with controller signals.

4. The device for preparing enzyme-catalyzed cartilage repair hydrogel according to claim 1, characterized in that: A visual window is embedded in the side wall of the reaction chamber, and the visual window is made of transparent material. A sampling port and a cover for closing the sampling port are opened on the side wall of the reaction chamber.

Images