Thermal spring water extract rich in specific trace elements and preparation method thereof

Abstract

The invention provides a thermal spring water extract rich in specific trace elements and a preparation method thereof. The preparation method comprises the following steps: (1) preparing a Sargassum ammoniac extract; (2) taking 300 mL of thermal spring water and 4-5 g of Sargassum ammoniac extract, uniformly mixing, sterilizing, and cooling to room temperature to obtain a fermentation substrate; (3) mixing the compound bacteria with thermal spring water to prepare 109CFU / mL bacterial liquid; inoculating 2-4mL of bacterial liquid into the fermentation substrate for fermentation culture, and culturing for 8-15 hours at the temperature of 40-45 DEG C; after fermentation culture is finished, sterilizing, and cooling to room temperature; centrifuging the sterilized and cooled fermentation liquor; and carrying out decoloring treatment, suction filtration and sterilization to obtain the thermal spring water extract rich in specific trace elements. The thermal spring water extract disclosed by the invention has a very good barrier repairing effect on skin.

Description

Technical Field

[0001] The invention belongs to the technical field of skin care products, and particularly relates to a hot spring water extract rich in specific trace elements and a preparation method thereof. Background Art

[0002] The skin constitutes one of the most important organs of the body and is considered one of the main active elements of the innate immune defense system. Healthy skin is able to defend itself against various external attacks, in particular due to its barrier and antimicrobial defense properties, and also its epidermal cell regeneration properties. However, continuous attacks by the environment, chemicals or radiation can lead to a gradual weakening of its ability to adapt and respond (especially in terms of the innate immune system). In the long run, these various attacks can have an inhibitory effect on the innate immune defense system, leading to a decrease in the barrier properties and defenses of the skin. These various attacks can also affect the epidermal cell regeneration properties and impair the process of epidermal renewal and healing.

[0003] Furthermore, during chronobiological or photoinduced aging, the innate immune defense system of the skin tends to become weaker. These breakdowns or impairments can be promoted or even aggravated by infections due to microorganisms and by the various aforementioned environmental insults (e.g. stress, UV radiation or "urban" living conditions).

[0004] Therefore, there is an urgent need for a hot spring water extract rich in specific trace elements and a preparation method thereof. Summary of the invention

[0005] The purpose of the present invention is to provide a hot spring water extract rich in specific trace elements and a preparation method thereof.

[0006] In order to achieve the above object, the present invention provides the following technical solutions: A method for preparing a hot spring water extract rich in specific trace elements, the preparation method comprising the following steps: (1) Wash the Naozhou Sargassum with clean water, dry it naturally, place it in an oven, dry it at 45-55° C. for 10-15 hours, crush it and pass it through a 50-mesh pharmacopoeia sieve to obtain Naozhou Sargassum powder; mix betaine and 1,3-butanediol in a molar ratio of 1:3-5, heat it at 75-85° C. and 300-500 rpm for 1-2 hours to obtain a low eutectic solvent, mix the Naozhou Sargassum powder and the low eutectic solvent in a mass ratio of 1:25-35, ultrasonicate it, centrifuge it at 1000-3000 rpm for 5-15 minutes to obtain a supernatant and a residue, recover the supernatant, and continue to extract the residue with the low eutectic solvent for 2-3 times, combine the supernatants, and concentrate them to obtain the Naozhou Sargassum extract; (2) Take 300 mL of hot spring water and 4-5 g of Sargassum naozhouense extract and mix them evenly, sterilize them and cool them to room temperature to obtain a fermentation substrate; (3) Mix the compound bacteria with hot spring water to make 10 9 CFU / mL of bacterial liquid; inoculating 2-4mL of bacterial liquid into the fermentation substrate for fermentation culture, and culturing at 40-45℃ for 8-15h; after the fermentation culture is completed, sterilize and then cool to room temperature; centrifuge the sterilized and cooled fermentation liquid; after the centrifugation is completed, take the supernatant, add activated carbon to the supernatant for decolorization, remove the decolorizer after the decolorization, first perform a suction filtration, perform a second suction filtration after the first suction filtration, and after the suction filtration is completed, obtain the decolorized fermentation stock solution, sterilize the decolorized fermentation stock solution, and obtain a hot spring water extract rich in specific trace elements.

[0007] Furthermore, the ultrasonic conditions in step (1) are: 240-280W ultrasonic power, 35-45°C, for 10-30min.

[0008] Furthermore, the sterilization conditions in step (2) are as follows: sterilization in a high temperature sterilizer at a temperature of 121°C and a pressure of 0.12 MPa for 30 minutes. Furthermore, the composite bacteria in step (3) include Lactobacillus brevis, Bifidobacterium longum and Lactobacillus plantarum.

[0009] Furthermore, in the step (3), the bacterial activity ratio of Lactobacillus brevis, Bifidobacterium longum and Lactobacillus plantarum in the bacterial solution is 1:1.3-1.5:0.4-0.7.

[0010] Furthermore, the centrifugal conditions in step (4) are: centrifugal speed 4500-5000 r / min, and centrifugal time 20-40 min.

[0011] Furthermore, in step (4), the mass volume ratio of activated carbon to supernatant is 8-12 g / L, the decolorization is carried out under stirring, the stirring speed is 100-300 rpm, and the decolorization time is 50-70 min. Furthermore, in the step (4), the filtration is performed once, and the pore size of the filter paper is 10 μm.

[0012] Furthermore, in the second filtration in step (4), the pore size of the filter paper is 0.45 μm.

[0013] The invention also provides a hot spring water extract rich in specific trace elements prepared by the preparation method.

[0014] Compared with the prior art, the advantages and beneficial effects of the present invention are: 1. The hot spring water of the specific trace elements of the present invention has the effect of repairing the skin barrier, mainly because the minerals and trace elements contained in these hot spring waters can provide a series of benefits that are beneficial to skin health. The minerals and trace elements in the hot spring water can help the skin retain moisture, enhance the skin's ability to lock water, make the skin softer and smoother, and reduce the phenomenon of dry skin and peeling. Hot spring water has a good soothing effect, can relieve skin inflammation and irritation, and reduce skin itching and discomfort. This is especially important for damaged skin barriers because it can help reduce inflammatory reactions and promote the natural healing process of the skin. Certain minerals in hot spring water, such as selenium, have antioxidant properties, which can help resist damage from free radicals and protect the skin from environmental factors, thereby helping to maintain the integrity of the skin barrier. Hot spring water helps promote blood circulation, increase the nutrient supply of skin cells, and accelerate the skin's repair process. The minerals and trace elements in hot spring water can enhance the skin's resistance, help the skin resist the influence of harmful external factors, and reduce the possibility of skin damage.

[0015] 2. Lactobacillus brevis, Bifidobacterium longum and Lactobacillus plantarum are all beneficial bacteria. During the fermentation process, they produce a variety of metabolites that are beneficial to skin health, such as lactic acid, short-chain fatty acids, peptide compounds, etc. These metabolites have antibacterial, anti-inflammatory and antioxidant effects, which help to improve the skin microenvironment and enhance the skin barrier function. Naozhou Sargassum is rich in a variety of beneficial ingredients, such as polysaccharides, proteins, amino acids, vitamins and minerals. These ingredients may be decomposed or converted into forms that are more easily absorbed by the skin during the fermentation process, thereby playing their role in repairing and nourishing the skin. In particular, ingredients such as polysaccharides and amino acids help moisturize and promote the repair of the skin barrier. When these beneficial bacteria are fermented in Naozhou Sargassum extract, there may be a synergistic effect between them, producing more beneficial metabolites. These metabolites can not only act directly on the skin, but also improve the overall health of the skin by regulating the balance of the skin microecology, promoting the growth of beneficial bacteria and inhibiting the reproduction of harmful bacteria. The multiple beneficial ingredients and active substances in these fermentation products can work together on the skin to help repair damaged skin barriers and improve the overall health of the skin by providing antioxidant, anti-inflammatory, moisturizing and promoting cell renewal. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a graph showing the detection results of trace elements in the hot spring water extract prepared in Example 1. DETAILED DESCRIPTION

[0017] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0018] Example 1 This embodiment provides a method for preparing a hot spring water extract rich in specific trace elements, the preparation method comprising the following steps: (1) Wash the Naozhou Sargassum with clean water, dry it naturally, place it in an oven, dry it at 50°C for 13 hours, crush it and pass it through a 50-mesh pharmacopoeia sieve to obtain Naozhou Sargassum powder; take betaine and 1,3-butanediol and mix them in a molar ratio of 1:4, heat them at 80°C and 400rpm for 1 hour to obtain a low eutectic solvent, mix the Naozhou Sargassum powder and the low eutectic solvent in a mass ratio of 1:30, ultrasonically treat it at 250W power and 40°C for 20 minutes, centrifuge it at 2000rpm for 10 minutes to obtain a supernatant and a residue, recover the supernatant, extract the residue with the low eutectic solvent twice, combine the supernatants, and concentrate them to obtain the Naozhou Sargassum extract; (2) 300 mL of hot spring water and 4.5 g of Sargassum naozhouense extract were mixed evenly, sterilized in a high-temperature sterilizer at a temperature of 121°C and a pressure of 0.12 MPa for 30 min, and cooled to room temperature after sterilization to obtain a fermentation substrate; (3) Mixing the composite bacteria with hot spring water, wherein the composite bacteria include Lactobacillus brevis, Bifidobacterium longum and Lactobacillus plantarum, and preparing 10 9 CFU / mL of bacterial liquid, the bacterial activity ratio of Lactobacillus brevis, Bifidobacterium longum and Lactobacillus plantarum in the bacterial liquid is 1:1.4:0.6; 3mL of bacterial liquid is inoculated into the fermentation substrate for fermentation culture, and cultured at 43°C for 10 hours; after the fermentation culture, sterilize in a high-temperature sterilizer at a temperature of 121°C and a pressure of 0.12MPa for 30 minutes, and then cool to room temperature; centrifuge the sterilized and cooled fermentation liquid at a centrifugal speed of 4800r / min and a centrifugal time of 30 minutes; take the supernatant after centrifugation, add activated carbon to the supernatant for Decolorization treatment, the mass volume ratio of activated carbon to supernatant is 10g / L, decolorization is carried out under stirring conditions, the stirring speed is 200rpm, the decolorization time is 60min, and the decolorant is removed after decolorization. First, a suction filtration is performed, and the pore size of the filter paper is 12μm. After the first filtration, a second filtration is performed, and the pore size of the filter paper is 0.22μm. After the filtration is completed, the decolorized fermentation stock solution is obtained, and the decolorized fermentation stock solution is sterilized. The sterilization conditions are 121℃, 30min, and the pressure is 0.12Mpa to obtain a hot spring water extract rich in specific trace elements.

[0019] Comparative Example 1 The difference between this comparative example and Example 1 is that the Sargassum naozhouense is replaced by green algae.

[0020] A method for preparing a hot spring water extract rich in specific trace elements, the preparation method comprising the following steps: (1) Wash the green algae with clean water, dry it naturally, place it in an oven, dry it at 50°C for 13 hours, crush it and pass it through a 50-mesh pharmacopoeia sieve to obtain green algae powder; mix betaine and 1,3-butanediol in a molar ratio of 1:4, heat it at 80°C and 400rpm for 1 hour to obtain a low eutectic solvent, mix the green algae powder and the low eutectic solvent in a mass ratio of 1:30, ultrasonically treat it at 250W power and 40°C for 20 minutes, and centrifuge it at 2000rpm for 10 minutes to obtain a supernatant and a residue, recover the supernatant, and continue to extract the residue with the low eutectic solvent twice, combine the supernatants, and concentrate them to obtain a green algae extract; (2) Take 300 mL of hot spring water and 4.5 g of green algae extract and mix them evenly, sterilize them in a high-temperature sterilizer at a temperature of 121°C and a pressure of 0.12 MPa for 30 min, and cool them to room temperature after sterilization to obtain a fermentation substrate; (3) Mixing the composite bacteria with hot spring water, wherein the composite bacteria include Lactobacillus brevis, Bifidobacterium longum and Lactobacillus plantarum, and preparing 10 9 CFU / mL of bacterial liquid, the bacterial activity ratio of Lactobacillus brevis, Bifidobacterium longum and Lactobacillus plantarum in the bacterial liquid is 1:1.4:0.6; 3mL of bacterial liquid is inoculated into the fermentation substrate for fermentation culture, and cultured at 43°C for 10 hours; after the fermentation culture, sterilize in a high-temperature sterilizer at a temperature of 121°C and a pressure of 0.12MPa for 30 minutes, and then cool to room temperature; centrifuge the sterilized and cooled fermentation liquid at a centrifugal speed of 4800r / min and a centrifugal time of 30 minutes; take the supernatant after centrifugation, add activated carbon to the supernatant for Decolorization treatment, the mass volume ratio of activated carbon to supernatant is 10g / L, decolorization is carried out under stirring conditions, the stirring speed is 200rpm, the decolorization time is 60min, and the decolorant is removed after decolorization. First, a suction filtration is performed, and the pore size of the filter paper is 12μm. After the first filtration, a second filtration is performed, and the pore size of the filter paper is 0.22μm. After the filtration is completed, the decolorized fermentation stock solution is obtained, and the decolorized fermentation stock solution is sterilized. The sterilization conditions are 121℃, 30min, and the pressure is 0.12Mpa to obtain a hot spring water extract rich in specific trace elements.

[0021] Comparative Example 2 The difference between this comparative example and Example 1 is that the composite bacteria are different.

[0022] A method for preparing a hot spring water extract rich in specific trace elements, the preparation method comprising the following steps: (1) Wash the Naozhou Sargassum with clean water, dry it naturally, place it in an oven, dry it at 50°C for 13 hours, crush it and pass it through a 50-mesh pharmacopoeia sieve to obtain Naozhou Sargassum powder; take betaine and 1,3-butanediol and mix them in a molar ratio of 1:4, heat them at 80°C and 400rpm for 1 hour to obtain a low eutectic solvent, mix the Naozhou Sargassum powder and the low eutectic solvent in a mass ratio of 1:30, ultrasonically treat it at 250W power and 40°C for 20 minutes, centrifuge it at 2000rpm for 10 minutes to obtain a supernatant and a residue, recover the supernatant, extract the residue with the low eutectic solvent twice, combine the supernatants, and concentrate them to obtain the Naozhou Sargassum extract; (2) 300 mL of hot spring water and 4.5 g of Sargassum naozhouense extract were mixed evenly, sterilized in a high-temperature sterilizer at a temperature of 121°C and a pressure of 0.12 MPa for 30 min, and cooled to room temperature after sterilization to obtain a fermentation substrate; (3) Mixing the composite bacteria with hot spring water, wherein the composite bacteria includes Lactobacillus bulgaricus, Lactobacillus helveticus and Bifidobacterium lactis, and preparing 10 9 CFU / mL of bacterial liquid, the bacterial activity ratio of Lactobacillus brevis, Bifidobacterium longum and Lactobacillus plantarum in the bacterial liquid is 1:1.4:0.6; 3mL of bacterial liquid is inoculated into the fermentation substrate for fermentation culture, and cultured at 43°C for 10 hours; after the fermentation culture, sterilize in a high-temperature sterilizer at a temperature of 121°C and a pressure of 0.12MPa for 30 minutes, and then cool to room temperature; centrifuge the sterilized and cooled fermentation liquid at a centrifugal speed of 4800r / min and a centrifugal time of 30 minutes; take the supernatant after centrifugation, add activated carbon to the supernatant for Decolorization treatment, the mass volume ratio of activated carbon to supernatant is 10g / L, decolorization is carried out under stirring conditions, the stirring speed is 200rpm, the decolorization time is 60min, and the decolorant is removed after decolorization. First, a suction filtration is performed, and the pore size of the filter paper is 12μm. After the first filtration, a second filtration is performed, and the pore size of the filter paper is 0.22μm. After the filtration is completed, the decolorized fermentation stock solution is obtained, and the decolorized fermentation stock solution is sterilized. The sterilization conditions are 121℃, 30min, and the pressure is 0.12Mpa to obtain a hot spring water extract rich in specific trace elements.

[0023] Comparative Example 3 The difference between this comparative example and Example 1 is that the bacterial activity ratio of Lactobacillus brevis, Bifidobacterium longum and Lactobacillus plantarum in the bacterial solution is 1:1:1.

[0024] A method for preparing a hot spring water extract rich in specific trace elements, the preparation method comprising the following steps: (1) Wash the Naozhou Sargassum with clean water, dry it naturally, place it in an oven, dry it at 50°C for 13 hours, crush it and pass it through a 50-mesh pharmacopoeia sieve to obtain Naozhou Sargassum powder; take betaine and 1,3-butanediol and mix them in a molar ratio of 1:4, heat them at 80°C and 400rpm for 1 hour to obtain a low eutectic solvent, mix the Naozhou Sargassum powder and the low eutectic solvent in a mass ratio of 1:30, ultrasonically treat it at 250W power and 40°C for 20 minutes, centrifuge it at 2000rpm for 10 minutes to obtain a supernatant and a residue, recover the supernatant, extract the residue with the low eutectic solvent twice, combine the supernatants, and concentrate them to obtain the Naozhou Sargassum extract; (2) 300 mL of hot spring water and 4.5 g of Sargassum naozhouense extract were mixed evenly, sterilized in a high-temperature sterilizer at a temperature of 121°C and a pressure of 0.12 MPa for 30 min, and cooled to room temperature after sterilization to obtain a fermentation substrate; (3) Mixing the composite bacteria with hot spring water, wherein the composite bacteria include Lactobacillus brevis, Bifidobacterium longum and Lactobacillus plantarum, and preparing 10 9 CFU / mL of bacterial liquid, the bacterial activity ratio of Lactobacillus brevis, Bifidobacterium longum and Lactobacillus plantarum in the bacterial liquid is 1:1:1; 3mL of bacterial liquid is inoculated into the fermentation substrate for fermentation culture, and cultured at 43°C for 10 hours; after the fermentation culture is completed, sterilize it in a high-temperature sterilizer at a temperature of 121°C and a pressure of 0.12MPa for 30 minutes, and then cool it to room temperature; centrifuge the sterilized and cooled fermentation liquid at a centrifugal speed of 4800r / min and a centrifugal time of 30 minutes; take the supernatant after centrifugation, and add activated carbon to the supernatant for decolorization Treatment, the mass volume ratio of activated carbon to supernatant is 10g / L, decolorization is carried out under stirring conditions, the stirring speed is 200rpm, the decolorization time is 60min, and the decolorizer is removed after decolorization. First, a suction filtration is performed, and the pore size of the filter paper is 12μm. After the first filtration, a second filtration is performed, and the pore size of the filter paper is 0.22μm. After the filtration is completed, the decolorized fermentation stock solution is obtained, and the decolorized fermentation stock solution is sterilized. The sterilization conditions are 121℃, 30min, and the pressure is 0.12Mpa to obtain a hot spring water extract rich in specific trace elements.

[0025] Comparative Example 4 The difference between this comparative example and Example 1 is that 3 mL of bacterial liquid is added to the fermentation substrate for fermentation culture, and the culture is carried out at 43° C. for 20 h.

[0026] A method for preparing a hot spring water extract rich in specific trace elements, the preparation method comprising the following steps: (1) Wash the Naozhou Sargassum with clean water, dry it naturally, place it in an oven, dry it at 50°C for 13 hours, crush it and pass it through a 50-mesh pharmacopoeia sieve to obtain Naozhou Sargassum powder; take betaine and 1,3-butanediol and mix them in a molar ratio of 1:4, heat them at 80°C and 400rpm for 1 hour to obtain a low eutectic solvent, mix the Naozhou Sargassum powder and the low eutectic solvent in a mass ratio of 1:30, ultrasonically treat it at 250W power and 40°C for 20 minutes, centrifuge it at 2000rpm for 10 minutes to obtain a supernatant and a residue, recover the supernatant, extract the residue with the low eutectic solvent twice, combine the supernatants, and concentrate them to obtain the Naozhou Sargassum extract; (2) 300 mL of hot spring water and 4.5 g of Sargassum naozhouense extract were mixed evenly, sterilized in a high-temperature sterilizer at a temperature of 121°C and a pressure of 0.12 MPa for 30 min, and cooled to room temperature after sterilization to obtain a fermentation substrate; (3) Mixing the composite bacteria with hot spring water, wherein the composite bacteria include Lactobacillus brevis, Bifidobacterium longum and Lactobacillus plantarum, and preparing 10 9 CFU / mL of bacterial liquid, the bacterial activity ratio of Lactobacillus brevis, Bifidobacterium longum and Lactobacillus plantarum in the bacterial liquid is 1:1.4:0.6; 3mL of bacterial liquid is inoculated into the fermentation substrate for fermentation culture, and cultured at 43°C for 20h; after the fermentation culture, sterilize in a high-temperature sterilizer at a temperature of 121°C and a pressure of 0.12MPa for 30min, and then cool to room temperature; centrifuge the sterilized and cooled fermentation liquid at a centrifugal speed of 4800r / min and a centrifugal time of 30min; take the supernatant after centrifugation, add activated carbon to the supernatant for Decolorization treatment, the mass volume ratio of activated carbon to supernatant is 10g / L, decolorization is carried out under stirring conditions, the stirring speed is 200rpm, the decolorization time is 60min, and the decolorant is removed after decolorization. First, a suction filtration is performed, and the pore size of the filter paper is 12μm. After the first filtration, a second filtration is performed, and the pore size of the filter paper is 0.22μm. After the filtration is completed, the decolorized fermentation stock solution is obtained, and the decolorized fermentation stock solution is sterilized. The sterilization conditions are 121℃, 30min, and the pressure is 0.12Mpa to obtain a hot spring water extract rich in specific trace elements.

[0027] Comparative Example 5 The difference between this comparative example and Example 1 is that 3 mL of bacterial liquid is added to the fermentation substrate for fermentation culture, and the culture is carried out at 43° C. for 4 h.

[0028] A method for preparing a hot spring water extract rich in specific trace elements, the preparation method comprising the following steps: (1) Wash the Naozhou Sargassum with clean water, dry it naturally, place it in an oven, dry it at 50°C for 13 hours, crush it and pass it through a 50-mesh pharmacopoeia sieve to obtain Naozhou Sargassum powder; take betaine and 1,3-butanediol and mix them in a molar ratio of 1:4, heat them at 80°C and 400rpm for 1 hour to obtain a low eutectic solvent, mix the Naozhou Sargassum powder and the low eutectic solvent in a mass ratio of 1:30, ultrasonically treat it at 250W power and 40°C for 20 minutes, centrifuge it at 2000rpm for 10 minutes to obtain a supernatant and a residue, recover the supernatant, extract the residue with the low eutectic solvent twice, combine the supernatants, and concentrate them to obtain the Naozhou Sargassum extract; (2) 300 mL of hot spring water and 4.5 g of Sargassum naozhouense extract were mixed evenly, sterilized in a high-temperature sterilizer at a temperature of 121°C and a pressure of 0.12 MPa for 30 min, and cooled to room temperature after sterilization to obtain a fermentation substrate; (3) Mixing the composite bacteria with hot spring water, wherein the composite bacteria include Lactobacillus brevis, Bifidobacterium longum and Lactobacillus plantarum, and preparing 10 9 CFU / mL of bacterial liquid, the bacterial activity ratio of Lactobacillus brevis, Bifidobacterium longum and Lactobacillus plantarum in the bacterial liquid was 1:1.4:0.6; 3mL of bacterial liquid was inoculated into the fermentation substrate for fermentation culture, and cultured at 43°C for 4h; after the fermentation culture, sterilized in a high-temperature sterilizer at a temperature of 121°C and a pressure of 0.12MPa for 30min, and then cooled to room temperature; centrifuged the sterilized and cooled fermentation liquid at a centrifugal speed of 4800r / min and a centrifugal time of 30min; after the centrifugation, the supernatant was taken, and activated carbon was added to the supernatant for Decolorization treatment, the mass volume ratio of activated carbon to supernatant is 10g / L, decolorization is carried out under stirring conditions, the stirring speed is 200rpm, the decolorization time is 60min, and the decolorant is removed after decolorization. First, a suction filtration is performed, and the pore size of the filter paper is 12μm. After the first filtration, a second filtration is performed, and the pore size of the filter paper is 0.22μm. After the filtration is completed, the decolorized fermentation stock solution is obtained, and the decolorized fermentation stock solution is sterilized. The sterilization conditions are 121℃, 30min, and the pressure is 0.12Mpa to obtain a hot spring water extract rich in specific trace elements.

[0029] Performance Testing 60 volunteers were selected as subjects, aged 20-45 years old, with healthy skin, no history of skin allergies, and met the voluntary inclusion criteria of the subjects. They were randomly divided into 6 groups for testing, and each group used a sample. The hot spring water extracts of Example 1 and Comparative Examples 1-5 were used as samples. The subjects sprayed an equal amount of the sample (2 mL g) evenly on the inner side of the forearm twice a day, morning and evening, and then the same tester used the TM300 tester of the German CK company to test the transepidermal water loss of the inner side of the forearm in the 0th week and after continuous use until the 4th week, referred to as TEWL, that is, how many grams of water are lost per square meter per hour.

[0030] TEWL is a very important parameter for evaluating the function of the skin's moisture barrier. It has been widely recognized internationally and can well reflect the integrity of the skin's barrier function. The standard TEWL is measured when the skin is dry and sweat-free, at an ambient temperature of 24°C and an ambient humidity of 50%. The test data are all obtained under these conditions.

[0031] The calculation formula of TEWL value decrease rate is as follows: TEWL value reduction rate (%) = (A0-An) / A0×100% Where A0 is the TEWL value of the 0th week, and An is the TEWL value of the nth week.

[0032] A positive TEWL value decrease rate indicates that the skin barrier has been repaired, otherwise, the skin barrier has not been repaired; and the greater the TEWL value decrease rate, the better the skin barrier repair effect. The calculation formula for the average TEWL value decrease rate is as follows: Average TEWL value reduction rate (%) = sum of TEWL value reduction rates of each group / number of people in the group The test results are shown in Table 1.

[0033] Table 1 Performance test results

[0034] From the above performance test results, it can be seen that Example 1 has outstanding skin barrier repair performance. However, since the comparative example does not adopt the necessary technical solution, it is obviously worse than the example in the corresponding performance test. The above experimental results further prove the importance of the technical solution defined in the present invention for its technical effect.

[0035] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for preparing a hot spring water extract rich in specific trace elements, characterized in that: The preparation method comprises the following steps: (1) Wash the Naozhou Sargassum with clean water, dry it naturally, place it in an oven, dry it at 45-55° C. for 10-15 hours, crush it and pass it through a 50-mesh pharmacopoeia sieve to obtain Naozhou Sargassum powder; mix betaine and 1,3-butanediol in a molar ratio of 1:3-5, heat it at 75-85° C. and 300-500 rpm for 1-2 hours to obtain a low eutectic solvent, mix the Naozhou Sargassum powder and the low eutectic solvent in a mass ratio of 1:25-35, ultrasonicate it, centrifuge it at 1000-3000 rpm for 5-15 minutes to obtain a supernatant and a residue, recover the supernatant, and continue to extract the residue with the low eutectic solvent for 2-3 times, combine the supernatants, and concentrate them to obtain the Naozhou Sargassum extract; (2) Take 300 mL of hot spring water and 4-5 g of Sargassum naozhouense extract and mix them evenly, sterilize them and cool them to room temperature to obtain a fermentation substrate; (3) Mix the compound bacteria with hot spring water to make 10 9 CFU / mL of bacterial liquid; inoculating 2-4mL of bacterial liquid into the fermentation substrate for fermentation culture, and culturing at 40-45℃ for 8-15h; after the fermentation culture is completed, sterilize and then cool to room temperature; centrifuge the sterilized and cooled fermentation liquid; after the centrifugation is completed, take the supernatant, add activated carbon to the supernatant for decolorization, remove the decolorizer after the decolorization, first perform a suction filtration, perform a second suction filtration after the first suction filtration, and after the suction filtration is completed, obtain the decolorized fermentation stock solution, sterilize the decolorized fermentation stock solution, and obtain a hot spring water extract rich in specific trace elements.

2. The method for preparing the hot spring water extract rich in specific trace elements according to claim 1, characterized in that: The ultrasonic conditions in step (1) are: ultrasonic power of 240-280 W, 35-45° C., for 10-30 min.

3. The method for preparing the hot spring water extract rich in specific trace elements according to claim 1, characterized in that: The sterilization conditions in step (2) are as follows: sterilization in a high-temperature sterilizer at a temperature of 121° C. and a pressure of 0.12 MPa for 30 minutes.

4. The method for preparing the hot spring water extract rich in specific trace elements according to claim 1, characterized in that: The composite bacteria in step (3) include Lactobacillus brevis, Bifidobacterium longum and Lactobacillus plantarum.

5. The method for preparing the hot spring water extract rich in specific trace elements according to claim 4, characterized in that: In the step (3), the bacterial activity ratio of Lactobacillus brevis, Bifidobacterium longum and Lactobacillus plantarum in the bacterial solution is 1:1.3-1.5:0.4-0.

7.

6. The method for preparing the hot spring water extract rich in specific trace elements according to claim 1, characterized in that: The centrifugal conditions in step (4) are: centrifugal speed 4500-5000 r / min, and centrifugal time 20-40 min.

7. The method for preparing the hot spring water extract rich in specific trace elements according to claim 1, characterized in that: In the step (4), the mass volume ratio of activated carbon to supernatant is 8-12 g / L, the decolorization is carried out under stirring, the stirring speed is 100-300 rpm, and the decolorization time is 50-70 min.

8. The method for preparing the hot spring water extract rich in specific trace elements according to claim 1, characterized in that: In the step (4), the filtration is performed once, and the pore size of the filter paper is 10 μm.

9. The method for preparing the hot spring water extract rich in specific trace elements according to claim 1, characterized in that: In the second filtration in step (4), the pore size of the filter paper is 0.45 μm.

10. A hot spring water extract rich in specific trace elements obtained by the preparation method according to any one of claims 1 to 9.

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