Soybean fermentation method for improving arterial calcification

Abstract

The invention belongs to the technical field of biology, and relates to a fermentation extraction process of vitamin K2, in particular to a soybean fermentation method for improving arterial calcification, which comprises the following steps: sequentially performing culture, separation, fermentation, solid-liquid separation, concentration, degerming, drying and the like on a bacillus subtilis strain to obtain the vitamin K2. The vitamin K2 can be added into modified milk powder, health food, sports nutrition, special dietary food, medicines and the like, and has the effects of supplementing nutrients, reversing arteriosclerosis, osteoporosis, inflammation, cancer, Alzheimer's disease and the like.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, relates to a vitamin K2 fermentation extraction process, and in particular to a soybean fermentation method for improving arterial calcification. Background Art

[0002] Vitamin K2 is a fat-soluble compound. Its all-trans configuration has extremely high biological activity. Its physiological function has always been considered to be related to blood coagulation. In recent years, it has been found that vitamin K2 can activate osteocalcin, which promotes the deposition of calcium in the blood into bones, and plays an important role in the prevention and treatment of osteoporosis. At the same time, vitamin K2 can activate the protein calcification inhibitor protein matrix γ-carboxyglutamate protein in blood vessels, thereby hindering the deposition of calcium in arterial blood vessels and inhibiting the differentiation of osteogenic factors, thereby inhibiting the calcification of blood vessels and soft tissues, preventing and reversing arteriosclerosis, and reducing the incidence of cardiovascular diseases. Vitamin K2 is mainly synthesized by bacteria, and its content in meat, dairy products and fermented foods is relatively low. In order to meet the human body's demand for vitamin K2, researchers have developed many bacteria as fermentation agents for food fermentation in the past decade, increasing the content of vitamin K2 in food. Researchers have found extremely rich vitamin K2 in the traditional Japanese food natto, which is a soybean product fermented with Bacillus natto. We use modern technology liquid fermentation method, use ultra-fine soybean powder, inoculate Bacillus subtilis to ferment and produce vitamin K2, and then separate, extract, purify and dry through multi-pass membrane technology to obtain vitamin K2 powder. As people's understanding of the key role of vitamin K2 gradually grows, studies have shown that vitamin K2 is closely related to many diseases, such as osteoporosis, inflammation, cancer, Alzheimer's disease, peripheral neuropathy, etc. Similarly, more and more studies have confirmed that vitamin K2 has a positive effect on cardiovascular health and is a low-cost and safe treatment option. Therefore, vitamin K2 has attracted much attention in the fields of medicine and functional foods. Summary of the invention

[0003] In view of the above problems, the present invention provides a soybean fermentation method for improving arterial calcification.

[0004] In order to achieve the above object, the technical solution adopted by the present invention is: A soybean fermentation method for improving arterial calcification comprises the following steps: A. Streak the Bacillus subtilis strain on the inclined surface of the seed culture medium and place it in a constant temperature box; B. Transfer the cultured bacteria from the slant to the seed tank for grinding; C. Prepare ultrafine soybean powder with purified water and put it into a fermentation tank, and inoculate the cultivated seeds into the fermentation tank for fermentation; D. After fermentation is finished, the fermentation liquid enters the separation ceramic membrane for solid-liquid separation and the clear liquid is taken; E. The clear liquid is concentrated by ultrafiltration membrane to obtain a concentrated liquid; F. Filter and sterilize with a PAll membrane, take the filtrate, and spray-dry the filtrate to obtain a high-content vitamin K2 powder raw material.

[0005] Preferably, in step A: The temperature is 36℃~40℃ and the incubation time is 8~12 h.

[0006] Preferably, in step B: The pH value is 6.9, the temperature is 36℃~40℃, the rotation speed of the seed tank is 200 r / min, and the culture time is 16~18h.

[0007] Preferably, the ratio and conditions in step C are: The ratio is: 10% inoculation volume, 60% fermentation tank liquid volume; pH value is 6.9, temperature is: 36℃~40℃, rotation speed is: 200 r / min, fermentation time is 6-8h.

[0008] Preferably, in step D: Filtration pressure: 0.2-0.3Mpa, frequency conversion system: 20Hz.

[0009] Preferably, in step E: Filtration pressure: 0.2-0.3Mpa, frequency conversion system: 20Hz.

[0010] The vitamin K2 extracted by fermentation in this patent can be added to formula milk powder, health food, sports nutrition, special dietary food, and medicine, so that it has the functions of supplementing nutrients, reversing arteriosclerosis, osteoporosis, inflammation, cancer, Alzheimer's disease, etc.

[0011] Compared with the prior art, the advantages and positive effects of the present invention are: The vitamin K2 fermentation and extraction process of soybean fermentation in the present invention can be added to formula milk powder, health food, sports nutrition, special dietary food, medicine, etc., and has the effects of supplementing nutrients, reversing arteriosclerosis, osteoporosis, inflammation, cancer, Alzheimer's disease, etc. DETAILED DESCRIPTION

[0012] In order to more clearly understand the above-mentioned purpose, features and advantages of the present invention, the present invention is further described below in conjunction with embodiments. It should be noted that the embodiments of the present application and the features in the embodiments can be combined with each other without conflict.

[0013] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways than those described herein. Therefore, the present invention is not limited to the specific embodiments of the following disclosure. Example

[0014] A soybean fermentation method for improving arterial calcification comprises the following steps: A. Streak the Bacillus subtilis strain on the inclined surface of the seed culture medium and place it in a constant temperature box; B. Transfer the cultured bacteria from the slant to the seed tank for grinding; C. Prepare ultrafine soybean powder with purified water and put it into a fermentation tank, and inoculate the cultivated seeds into the fermentation tank for fermentation; D. After fermentation is finished, the fermentation liquid enters the separation ceramic membrane for solid-liquid separation and the clear liquid is taken; E. The clear liquid is concentrated by ultrafiltration membrane to obtain a concentrated liquid; F. Filter and sterilize with a PAll membrane, take the filtrate, and spray-dry the filtrate to obtain a high-content vitamin K2 powder raw material.

[0015] Preferably, in step A: The temperature is 36℃~40℃ and the incubation time is 8~12 h.

[0016] In step B: The pH value is 6.9, the temperature is 36℃~40℃, the rotation speed of the seed tank is 200 r / min, and the culture time is 16~18h.

[0017] The ratio and conditions in step C are: The ratio is: 10% inoculation volume, 60% fermentation tank liquid volume; pH value is 6.9, temperature is: 36℃~40℃, rotation speed is: 200 r / min, fermentation time is 6-8h.

[0018] In step D: Filtration pressure: 0.2-0.3Mpa, frequency conversion system: 20Hz.

[0019] In step E: Filtration pressure: 0.2-0.3Mpa, frequency conversion system: 20Hz.

[0020] The vitamin K2 extracted by fermentation in this patent can be added to formula milk powder, health food, sports nutrition, special dietary food, and medicine, so that it has the functions of supplementing nutrients, reversing arteriosclerosis, osteoporosis, inflammation, cancer, Alzheimer's disease, etc.

[0021] The above description is only a preferred embodiment of the present invention and does not limit the present invention in other forms. Any technician familiar with the profession may use the technical content disclosed above to change or modify it into an equivalent embodiment with equivalent changes and apply it to other fields. However, any simple modification, equivalent change and modification made to the above embodiment based on the technical essence of the present invention without departing from the content of the technical solution of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A soybean fermentation method for improving arterial calcification, characterized in that: The specific steps include: A. Streak the Bacillus subtilis strain on the inclined surface of the seed culture medium and place it in a constant temperature box; B. Transfer the cultured bacteria from the slant to the seed tank for grinding; C. Prepare ultrafine soybean powder with purified water and put it into a fermentation tank, and inoculate the cultivated seeds into the fermentation tank for fermentation; D. After fermentation is finished, the fermentation liquid enters the separation ceramic membrane for solid-liquid separation and the clear liquid is taken; E. The clear liquid is concentrated by ultrafiltration membrane to obtain a concentrated liquid; F. Filter and sterilize with a PAll membrane, take the filtrate, and spray-dry the filtrate to obtain a high-content vitamin K2 powder raw material.

2. The soybean fermentation method for improving arterial calcification according to claim 1, characterized in that: In step A: The temperature is 36℃~40℃ and the incubation time is 8~12 h.

3. The soybean fermentation method for improving arterial calcification according to claim 2, characterized in that: In step B: The pH value is 6.9, the temperature is 36℃~40℃, the rotation speed of the seed tank is 200 r / min, and the culture time is 16~18 h.

4. The soybean fermentation method for improving arterial calcification according to claim 3, characterized in that: The ratio and conditions in step C are: The ratio is: 10% inoculation volume, 60% fermentation tank liquid volume; pH value is 6.9, temperature is: 36℃~40℃, rotation speed is: 200 r / min, fermentation time is 6-8h.

5. The soybean fermentation method for improving arterial calcification according to claim 4, characterized in that: In step D: Filtration pressure: 0.2-0.3Mpa, frequency conversion system: 20Hz.

6. The soybean fermentation method for improving arterial calcification according to claim 5, characterized in that: In step E: Filtration pressure: 0.2-0.3Mpa, frequency conversion system: 20Hz.

7. The vitamin K2 extracted by fermentation in claims 1-6 can be added to formula milk powder, health food, sports nutrition, special dietary food, and medicine to make it have the functions of supplementing nutrients, reversing arteriosclerosis, osteoporosis, inflammation, cancer, and Alzheimer's disease.