A single channel pipette and method of use thereof

Abstract

The application discloses a single-channel pipette and a use method thereof, and particularly relates to the technical field of single-channel pipettes, which comprises an upper gun body and a lower gun body arranged at the bottom of the upper gun body, a protection connecting assembly is arranged between the upper gun body and the lower gun body, the protection connecting assembly comprises a center cylinder arranged in the upper gun body, and a piston cylinder is arranged at the bottom of the center cylinder and located outside the center cylinder. Through the corresponding cooperation of various structures, when the second limiting tooth and the first limiting tooth slide, a meshing sound with equal tooth spacing is emitted, which prompts the operator that the upper gun body and the lower gun body will reach a screwed state, the limiting buckle is clamped in the buckle cavity and emits an in-place sound, which prompts the operator that the screwing has reached the in-place state, the center cylinder cannot be continuously screwed, the operator is prevented from over-tightening the wire sleeve and causing the wire sleeve to burst, the hidden danger that the lower gun body falls off due to the bursting of the wire sleeve is prevented, and dangerous accidents caused by liquid leakage are avoided.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of single-channel pipette, more particularly, the present application relates to a single-channel pipette and a method for using the same. BACKGROUND

[0002] A pipette is a tool used to accurately move and transfer liquids in a laboratory. It is usually composed of a long and thin tube with a suction device at one end and a liquid release nozzle at the other end. Pipettes can take and release a predetermined amount of liquid by operating the piston of the suction device. They are commonly used in biochemical, molecular biology, pharmaceutical and other laboratory work to prepare solutions, process samples, mix reactions, etc. The capacity of pipettes can vary from microliters to milliliters, and according to the number of liquid taking nozzles, pipettes are divided into single-channel pipettes and multi-channel pipettes.

[0003] The upper half of the gun body of the single-channel pipette product is fixed to the lower half of the gun head by connection, and most of the products on the market use buckle type or threaded connection. The buckle type connection can achieve simple connection and fixation, but the buckle has a certain virtual position, so the lower half of the gun head will be loose, which will affect the accuracy of the pipette. At the same time, the buckle is relatively weak, and the buckle has little space, which can easily cause the lower half of the gun head to fall off during use, which not only wastes valuable experimental samples, but also causes great harm to the operator. The threaded connection can achieve fixation, but most of them do not have an anti-loose device, which can cause the threads to loosen and fall off during use, which can easily affect the accuracy of the pipette and cause great harm to the operator. In addition, there is no early warning measure during the tightening process, which can cause the pipette head or the upper half of the gun body to explode during tightening, which can also cause safety hazards and accidents. SUMMARY

[0004] In order to overcome the above-mentioned defects of the prior art, the present application provides a single-channel pipette and a method for using the same, which aims to solve the problems raised in the background art.

[0005] To achieve the above-mentioned purpose, the present application provides the following technical solution: a single-channel pipette comprising an upper gun body and a lower gun body arranged at the bottom of the upper gun body, wherein a protective connection assembly is arranged between the upper gun body and the lower gun body.

[0006] The protective connection assembly comprises a center cylinder arranged in the upper gun body, a piston cylinder arranged outside the center cylinder at the bottom of the center cylinder, an annular buckle arranged at the bottom of the center cylinder, a plurality of first limiting teeth for limiting arranged at the bottom of the annular buckle and distributed along the circumferential point of the axis of the annular buckle, a limiting seat for limiting arranged in the piston cylinder, a deformation cavity arranged on both sides of the surface of the limiting seat, a buckle cavity for limiting arranged on both sides of the inner wall of the piston cylinder at the bottom, and each of the buckle cavities extending to the surface of the piston cylinder, a limiting buckle for limiting arranged on both sides of the bottom of the limiting seat, the limiting buckle being matched with the buckle cavity, and each of the limiting buckles extending into the corresponding buckle cavity and being clamped with the buckle cavity, a second limiting tooth arranged on the top of the limiting buckle and engaged with the first limiting tooth, and a thread sleeve arranged on the inner wall of the piston cylinder and the outside of the center cylinder and locked by threads; As can be seen from the above technical solution, the first limiting tooth on the end surface of the annular buckle and the second limiting tooth on the limiting buckle collide, when the limiting seat is compressed, the rubber pad has a longitudinal elastic space, so when the second limiting tooth and the first limiting tooth slide, the engagement sound with the same tooth spacing will be emitted, prompting the operator that the upper gun body and the lower gun body will reach the tightened state, the limiting buckle is clamped in the buckle cavity and emits the in-place sound, prompting the operator that the tightening has reached the in-place position, and the center cylinder cannot be continuously twisted due to the limiting buckle and the buckle cavity, thereby avoiding the operator from over-tightening the thread sleeve and preventing the thread sleeve from being blown off, which avoids the hidden danger of the lower gun body falling off and the dangerous accident caused by liquid leakage;

[0007] The bottom of the limiting seat is arranged with a rubber pad, the bottom of the rubber pad is arranged with a spring arranged in the piston cylinder, the bottom of the rubber pad and outside the spring is arranged with a limiting block, the limiting block is arranged at the bottom of the inner wall of the piston cylinder, the bottom of the limiting block is arranged with a sealing ring, the inside of the spring is arranged with a piston rod, the bottom of the piston rod penetrates the spring and extends into the lower gun body, the top of the lower gun body and outside the piston cylinder is arranged with a push sleeve, the top of the push sleeve extends into the upper gun body and is detachably connected with the upper gun body, the top of the upper gun body is arranged with a hand push sleeve, one side of the hand push sleeve is arranged in an arc shape in vertical cross section, and the top of the hand push sleeve is arranged with a delivery pipe communicated with the center cylinder;

[0008] As can be seen from the above technical solution, the rubber pad can make the second limiting tooth and the first limiting tooth in stable engagement force when vibrating, so that the first limiting tooth and the second limiting tooth will not be loosened and the limiting seat will not be separated from the piston cylinder when vibrating, thereby effectively solving the problem of loosening of the lower gun body caused by long-term use or vibration, not only solving the problem of unstable precision caused by loosening of the pipette, but also effectively solving the problem of liquid spilling caused by loosening of the thread, thereby avoiding the occurrence of dangerous accidents; a use method, using the single-channel pipette, the method comprising the following steps,

[0009] Step one, when installing, the upper gun body and the lower gun body are screwed together through the wire sleeve outside the center tube and the wire sleeve inside the piston tube wall, when screwed to a certain depth, the first limiting teeth on the annular buckle end face and the second limiting teeth on the limiting buckle collide, since the limiting seat is compressed, the rubber pad has a longitudinal elastic space, so when the second limiting teeth and the first limiting teeth slide, the meshing sound of equal tooth spacing is emitted, prompting the operator that the upper gun body and the lower gun body will reach the screwed state;

[0010] Step two, when continuing to screw to the bottom, the limiting buckle will be clamped in the buckle cavity and emit a sound that the operation is in place, prompting the operator that the screwing has reached the position, and since the limiting buckle and the buckle cavity, the center tube cannot continue to be screwed, thereby avoiding the operator from over-tightening the wire sleeve and preventing the hidden danger of the lower gun body falling off caused by the wire sleeve explosion, and avoiding dangerous accidents caused by liquid leakage;

[0011] Step three, the limiting seat is installed on the piston tube through the limiting buckle and the buckle cavity, so that the limiting seat cannot rotate, but there is a little virtual position below the buckle cavity, which can move longitudinally by a certain displacement, and the limiting buckle can have a certain elasticity through the deformation cavity, under the action of the thread torque, and the limiting seat is installed on the piston tube through the buckle cavity and the limiting buckle, and the limiting seat is pressed on the rubber pad, so that the limiting seat has a longitudinal elastic space;

[0012] Step four, when vibrating, the rubber pad can make the second limiting teeth and the first limiting teeth have a stable meshing force, so that when vibrating, the first limiting teeth and the second limiting teeth will not be loosened, and the limiting seat will not be separated from the piston tube, thereby effectively solving the problem of the lower gun body loosening caused by long-term use or vibration, not only solving the problem of unstable precision caused by the loosening of the pipette, but also effectively solving the problem of liquid spilling caused by the loosening of the thread, and avoiding the occurrence of dangerous accidents.

[0013] Technical effects and advantages of the present application:

[0014] The upper gun body and the lower gun body are screwed together through the wire sleeve outside the center tube and the wire sleeve inside the piston tube wall, when screwed to a certain depth, the first limiting teeth on the annular buckle end face and the second limiting teeth on the limiting buckle collide, so that the upper gun body and the lower gun body can be connected together;

[0015] When the limiting seat is compressed, the rubber pad has a longitudinal elastic space, so when the second limiting teeth and the first limiting teeth slide, the meshing sound of equal tooth spacing is emitted, prompting the operator that the upper gun body and the lower gun body will reach the screwed state;

[0016] The present application can avoid the operator from over-tightening the silk sleeve, prevent the silk sleeve from being broken due to over-tightening, and avoid the hidden danger of the lower gun body falling off due to the broken silk sleeve, and avoid the dangerous accidents caused by liquid leakage.

[0017] The limiting seat is installed on the piston cylinder through the limiting buckle and the buckle cavity, so that the limiting seat cannot rotate and can move longitudinally by a certain displacement, the limiting buckle has a certain elasticity through the deformation cavity, under the action of the thread torque, the limiting seat is installed on the piston cylinder through the buckle cavity and the limiting buckle, and the limiting seat is pressed on the rubber pad, so that the limiting seat has a longitudinal elastic space.

[0018] When the present application vibrates, the rubber pad can make the second limiting tooth and the first limiting tooth have stable meshing force, so that the first limiting tooth and the second limiting tooth will not be loosened during vibration, and the limiting seat will not be separated from the piston cylinder, thereby effectively solving the problem of the lower gun body loosening caused by long-term use or vibration.

[0019] Through the cooperation of various structures, the first limiting tooth on the annular buckle end surface and the second limiting tooth on the limiting buckle collide, so that the upper gun body and the lower gun body can be connected together, when the limiting seat is compressed, the rubber pad has a longitudinal elastic space, so that the second limiting tooth and the first limiting tooth will emit meshing sound with equal tooth spacing when sliding, prompting the operator that the upper gun body and the lower gun body will reach the tightened state.

[0020] The limiting buckle is clamped in the buckle cavity and emits a sound when in place, prompting the operator that the tightening has reached the position, so that the central cylinder cannot continue to be twisted, thereby avoiding the operator from over-tightening the silk sleeve, preventing the lower gun body from falling off due to the broken silk sleeve, and avoiding dangerous accidents caused by liquid leakage. BRIEF DESCRIPTION OF DRAWINGS

[0021] In order to more clearly illustrate the technical solutions in the present disclosure, the drawings needed to be used in some embodiments of the present disclosure will be briefly introduced as follows. Obviously, the drawings in the following description are only some drawings of the embodiments of the present disclosure, and other drawings can also be obtained by those skilled in the art based on these drawings. In addition, the drawings in the following description can be regarded as schematic diagrams, and are not limited to the actual size, actual process, actual timing of signals, etc. of the products involved in the embodiments of the present disclosure.

[0022] Fig. 1 is a schematic diagram of the overall structure of the present application.

[0023] Fig. 2 is a sectional view of the present application with the center cylinder installed in the upper gun body.

[0024] Fig. 3 is a sectional view of the present application with the limiting seat, rubber pad and spring.

[0025] Fig. 4 is an exploded view of the present application. Figure 3

[0026] Fig. 5 is a front view of the present application with the center cylinder, ring buckle, first limiting tooth and upper gun body.

[0027] Fig. 6 is a front view of the present application with the push sleeve, second limiting tooth, limiting seat and center cylinder.

[0028] Fig. 7 is a front view of the present application with the piston cylinder, limiting seat, limiting buckle and second limiting tooth.

[0029] Fig. 8 is a front view of the present application with the center cylinder and various structures on the limiting seat.

[0030] The reference signs are as follows: 1, upper gun body; 101, lower gun body; 102, hand push sleeve; 103, delivery pipe; 2, center cylinder; 201, piston cylinder; 202, ring buckle; 203, first limiting tooth; 204, limiting seat; 205, deformation cavity; 206, buckle cavity; 207, limiting buckle; 208, second limiting tooth; 209, wire sleeve; 3, rubber pad; 301, spring; 302, limiting block; 303, sealing ring; 304, piston rod; 305, push sleeve. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present application will be described clearly and completely in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, but not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0032] ​The terms "first", "second", "third", etc. in the embodiments of the present application are used only for the purpose of description, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features defined with "first", "second", "third" can explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "multiple" is at least two, for example, two, three, etc., unless otherwise explicitly and specifically limited. In addition, the terms "include" and "have" and any variations thereof are intended to cover non-exclusive inclusion. For example, a process, method, system, product or device including a series of steps or units is not limited to the listed steps or units, but can optionally further include steps or units not listed, or can optionally further include other steps or units inherent to the process, method, product or device.

[0033] Reference herein to "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment can be included in at least one embodiment of the application. The occurrence of the phrase in various places in the specification is not necessarily all referring to the same embodiment, nor is it necessarily referring to a separate or alternative embodiment. It is explicitly and implicitly understood that the embodiments described herein can be combined with other embodiments.

[0034] A single-channel pipette and its use method as shown in FIGS. 1-8, through the protective connection assembly arranged between the upper gun body 1 and the center cylinder 2, through the collision of the first limiting teeth 203 on the end face of the annular buckle 202 and the second limiting teeth 208 on the limiting buckle 207, the upper gun body 1 and the lower gun body 101 can be easily connected together, when the limiting seat 204 is compressed, the rubber pad 3 has a longitudinal elastic space, so when the second limiting teeth 208 and the first limiting teeth 203 slide, the meshing sound with equal tooth spacing will be emitted, prompting the operator that the upper gun body 1 and the lower gun body 101 will reach the tightened state, through the limiting buckle 207 being buckled in the buckle cavity 206 and emitting the in-place sound, prompting the operator that the tightening has reached the in-place position, so that the center cylinder 2 cannot continue to be twisted, thereby avoiding the operator over-tightening the thread sleeve 209, preventing the thread sleeve 209 from being twisted off and causing the lower gun body 101 to fall off, avoiding dangerous accidents caused by liquid leakage, the rubber pad 3 can make the second limiting teeth 208 and the first limiting teeth 203 in stable meshing force, so that when vibrating, the first limiting teeth 203 and the second limiting teeth 208 will not be loosened, and the limiting seat 204 will not be separated from the piston cylinder 201, thus effectively solving the problem of the lower gun body 101 loosening caused by long-term use or vibration, not only solving the problem of unstable precision caused by the loosening of the pipette, but also effectively solving the problem of liquid spilling caused by the loosening of the thread, avoiding the occurrence of dangerous accidents, and the specific structure of the assembly is as follows.

[0035] The protective connection assembly comprises a center cylinder 2 arranged in the upper gun body 1, the bottom of the center cylinder 2 is provided with a piston cylinder 201 located outside the center cylinder 2, the bottom end of the center cylinder 2 is provided with an annular buckle 202, and the bottom of the annular buckle 202 is circumferentially distributed around the axis point of the annular buckle 202 and has a plurality of first limiting teeth 203 for limiting; the piston cylinder 201 is provided with a limiting seat 204 for limiting, the surface of the limiting seat 204 is provided with a deformation cavity 205 penetrating on both sides, the inner wall of the piston cylinder 201 is provided with a buckle cavity 206 for limiting on both sides of the bottom, and each buckle cavity 206 extends to the surface of the piston cylinder 201, the bottom of the limiting seat 204 is provided with a limiting buckle 207 for limiting on both sides, the limiting buckle 207 is matched with the buckle cavity 206, and each limiting buckle 207 extends into the corresponding buckle cavity 206 and is clamped with the buckle cavity 206, the top of the limiting buckle 207 is provided with a second limiting tooth 208 engaged with the first limiting tooth 203, and the inner wall of the piston cylinder 201 and the outside of the center cylinder 2 are provided with a thread sleeve 209 for locking;

[0036] The bottom of the limiting seat 204 is provided with a rubber pad 3 inside the piston cylinder 201, the bottom of the rubber pad 3 is provided with a spring 301 inside the piston cylinder 201, the bottom of the rubber pad 3 outside the spring 301 is provided with a limiting pressing block 302, the limiting pressing block 302 is located at the bottom of the inner wall of the piston cylinder 201, the bottom of the limiting pressing block 302 is provided with a sealing ring 303, the inside of the spring 301 is provided with a piston rod 304, the bottom of the piston rod 304 penetrates the spring 301 and extends into the lower gun body 101, the top of the lower gun body 101 outside the piston cylinder 201 is provided with a push sleeve 305, the top of the push sleeve 305 extends into the upper gun body 1 and is detachably connected with the upper gun body 1, the top of the upper gun body 1 is provided with a hand push sleeve 102, one side of the hand push sleeve 102 is vertically cut into an arc shape, the top of the hand push sleeve 102 is provided with a delivery pipe 103 in communication with the center cylinder 2;

[0037] A use method using the single-channel pipette gun, the method comprising the following steps,

[0038] Step one, when installing, the upper gun body 1 and the lower gun body 101 are screwed together through the wire sleeve 209 outside the center cylinder 2 and the wire sleeve 209 on the inner wall of the piston cylinder 201, when screwed to a certain depth, the first limiting tooth 203 on the end face of the annular buckle 202 collides with the second limiting tooth 208 on the limiting buckle 207, because the rubber pad 3 has a longitudinal elastic space when compressed, when the second limiting tooth 208 and the first limiting tooth 203 slide, the meshing sound of equal tooth spacing is emitted, prompting the operator that the upper gun body 1 and the lower gun body 101 will reach the screwed state;

[0039] Step two, when continuing to screw to the bottom, the limiting buckle 207 will be clamped in the buckle cavity 206 and emit a sound to the position, prompting the operator that the tightening has reached the position, and because of the limiting buckle 207 and the buckle cavity 206, the center cylinder 2 cannot continue to be screwed, thereby avoiding the operator from over-tightening and causing the wire sleeve 209 to burst, preventing the hidden danger of the lower gun body 101 falling off caused by the bursting of the wire sleeve 209, and avoiding dangerous accidents caused by liquid leakage;

[0040] Step three, while the limiting seat 204 is installed on the piston cylinder 201 through the limiting buckle 207 and the buckle cavity 206, so that the limiting seat 204 cannot rotate, but there is a little virtual position below the buckle cavity 206, which can move longitudinally within a certain displacement, while the limiting buckle 207 can have a certain elasticity through the deformation cavity 205, under the action of the matching thread torsion force, and the limiting seat 204 is installed on the piston cylinder 201 through the buckle cavity 206 and the limiting buckle 207, and the limiting seat 204 is pressed on the rubber pad 3, so that the limiting seat 204 has a certain elastic space for longitudinal movement;

[0041] Step four, when vibrating, the rubber pad 3 can make the second limiting tooth 208 and the first limiting tooth 203 in stable engagement force, so that when vibrating, it will not cause the first limiting tooth 203 and the second limiting tooth 208 to loosen, nor will it cause the limiting seat 204 to be separated from the piston cylinder 201, thus effectively solving the problem of loosening of the lower gun body 101 caused by long-term use or vibration, not only solving the problem of unstable precision caused by loosening of the pipette, but also effectively solving the problem of liquid spilling caused by loosening of the thread, avoiding the occurrence of dangerous accidents.

[0042] According to the above structure, when in use, the upper gun body 1 and the lower gun body 101 are screwed together through the wire sleeve 209 outside the center tube 2 and the wire sleeve 209 inside the wall of the piston tube 201. When screwed to a certain depth, the first limiting teeth 203 on the end face of the annular buckle 202 and the second limiting teeth 208 on the limiting buckle 207 collide. Since the limiting seat 204 has a longitudinal elastic space when compressed, when the second limiting teeth 208 and the first limiting teeth 203 slide, the meshing sound of equal tooth spacing is emitted, prompting the operator that the upper gun body 1 and the lower gun body 101 will reach the screwed state; when continuing to screw to the bottom, the limiting buckle 207 will be clamped in the buckle cavity 206 and emit a sound to the position, prompting the operator that the tightening has reached the position, and due to the limiting buckle 207 and the buckle cavity 206, the center tube 2 cannot continue to be screwed, thereby avoiding the operator from over-tightening and causing the wire sleeve 209 to burst, preventing the wire sleeve 209 from bursting and causing the lower gun body 101 to fall off, and avoiding dangerous accidents caused by liquid leakage; the limiting seat 204 is installed on the piston tube 201 through the limiting buckle 207 and the buckle cavity 206, so that the limiting seat 204 cannot rotate, but there is a little virtual position below the buckle cavity 206, which can move longitudinally by a certain displacement, and the limiting buckle 207 can have a certain elasticity through the deformation cavity 205, under the action of the thread torque, the limiting seat 204 is installed on the piston tube 201 through the buckle cavity 206 and the limiting buckle 207, and the limiting seat 204 is pressed on the rubber pad 3, so that the limiting seat 204 has a longitudinal elastic space; the rubber pad 3 can make the second limiting teeth 208 and the first limiting teeth 203 have a stable meshing force when vibrating, so that the first limiting teeth 203 and the second limiting teeth 208 do not loosen, and the limiting seat 204 does not separate from the piston tube 201, thus effectively solving the problem of loosening of the lower gun body 101 caused by long-term use or vibration. Not only can it solve the problem of unstable precision caused by loosening of the pipette, but also effectively solve the problem of liquid spilling caused by loosening of the thread, and avoid dangerous accidents.

[0043] Different from the prior art, the application discloses a single-channel pipette and a use method thereof. Through the collision of the first limiting tooth 203 on the end surface of the annular buckle 202 and the second limiting tooth 208 on the limiting buckle 207, the upper gun body 1 and the lower gun body 101 can be connected together. When the limiting seat 204 is compressed, the rubber pad 3 has a longitudinal elastic space, so when the second limiting tooth 208 and the first limiting tooth 203 slide, the meshing sound with equal tooth spacing will be emitted, prompting the operator that the upper gun body 1 and the lower gun body 101 will reach the tightened state. Through the limiting buckle 207 being clamped in the buckle cavity 206 and emitting the in-place sound, the operator is prompted that the tightening has reached the in-place position, so that the central cylinder 2 cannot continue to be twisted, thereby avoiding the operator from over-tightening the wire sleeve 209, preventing the wire sleeve 209 from being twisted off and causing the lower gun body 101 to fall off, avoiding the dangerous accidents caused by liquid leakage, the rubber pad 3 can make the second limiting tooth 208 and the first limiting tooth 203 be in stable meshing force, so that when vibrating, the first limiting tooth 203 and the second limiting tooth 208 will not be loosened, and the limiting seat 204 will not be separated from the piston cylinder 201, thus effectively solving the problem that the lower gun body 101 is loosened due to long-time use or vibration. Not only can the problem of unstable precision caused by the loosening of the pipette be solved, but also the problem of liquid spilling caused by the loosening and falling off of the thread can be effectively solved, and the occurrence of dangerous accidents is avoided.

[0044] The above is only the preferred embodiment of the application and is not used to limit the application. Any modification, equivalent replacement, improvement, etc. within the spirit and principle of the application shall be included in the protection scope of the application.

Claims

1. A single channel pipette comprising an upper body (1) and a lower body (101) arranged at the bottom of the upper body (1), characterized in that: The protective connection assembly is arranged between the upper gun body (1) and the lower gun body (101); The protective connection assembly comprises a center cylinder (2) arranged in the upper gun body (1), the bottom of the center cylinder (2) is provided with a piston cylinder (201) located outside the center cylinder (2), the bottom end of the center cylinder (2) is provided with an annular buckle (202), and the bottom of the annular buckle (202) and along the axis of the annular buckle (202) is circumferentially provided with a plurality of first limiting teeth (203) for limiting; The piston cylinder (201) is provided with a limiting seat (204) for limiting inside, and deformation cavities (205) are formed on both sides of the surface of the limiting seat (204); The inner wall bottom of the piston cylinder (201) is provided with buckle cavities (206) for limiting on both sides, and each buckle cavity (206) extends to the surface of the piston cylinder (201); The bottom of the limiting seat (204) is provided with limiting buckles (207) for limiting on both sides, the limiting buckles (207) are matched with the buckle cavities (206), and each limiting buckle (207) extends into the corresponding buckle cavity (206) and is clamped with the buckle cavity (206), and the top of the limiting buckle (207) is provided with second limiting teeth (208) engaged with the first limiting teeth (203); The inner wall of the piston cylinder (201) and the outer side of the center cylinder (2) are provided with thread-locked silk sleeves (209).

2. The single channel pipette of claim 1, wherein: The bottom of the limiting seat (204) and inside the piston cylinder (201) is provided with a rubber pad (3), and the bottom of the rubber pad (3) is provided with a spring (301) located inside the piston cylinder (201).

3. The single channel pipette of claim 2, wherein: The bottom of the rubber pad (3) and outside the spring (301) is provided with a limiting pressing block (302), the limiting pressing block (302) is located at the bottom of the inner wall of the piston cylinder (201), and the bottom of the limiting pressing block (302) is provided with a sealing ring (303).

4. The single channel pipette of claim 2, wherein: The inside of the spring (301) is provided with a piston rod (304), the bottom of the piston rod (304) penetrates the spring (301) and extends into the lower gun body (101).

5. The single channel pipette of claim 1, wherein: The top of the lower gun body (101) and outside the piston cylinder (201) is provided with a push sleeve (305), the top of the push sleeve (305) extends into the upper gun body (1) and is detachably connected with the upper gun body (1), and the push sleeve (305) is used for detaching the suction head.

6. The single channel pipette of claim 1, wherein: The top of the upper gun body (1) is provided with a hand push sleeve (102), one side of the hand push sleeve (102) is vertically provided in an arc shape, and the top of the hand push sleeve (102) is provided with a conveying pipe (103) in communication with the center cylinder (2).

7. A method of use, using the single channel pipette of any one of claims 1-6, characterized by: The method comprises the following steps, Step one, the upper gun body (1) and the lower gun body (101) are screwed together through the silk sleeves (209) outside the center cylinder (2) and the silk sleeves (209) on the inner wall of the piston cylinder (201), the first limiting teeth (203) on the end surface of the annular buckle (202) and the second limiting teeth (208) on the limiting buckle (207) collide, emit an equal-interval kneading sound, and are engaged in pre-tightening; Step two, the limit seat (204) is installed on the piston cylinder (201) through the limit buckle (207) and the buckle cavity (206), so that the limit seat (204) cannot rotate, and under the cooperation of the thread torsion, the limit seat (204) is installed on the piston cylinder (201) through the buckle cavity (206) and the limit buckle (207); Step three, when vibrating, the rubber pad (3) can make the second limit tooth (208) and the first limit tooth (203) be in stable engagement force, so that when vibrating, the first limit tooth (203) and the second limit tooth (208) will not be loosened, and the limit seat (204) will not be separated from the piston cylinder (201), so that the upper gun body (1) and the lower gun body (101) are connected and fastened, and will not be loosened when vibrating.

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