Nucleic Acid Integrated Detection Device Based on Flexible Structure and Push-Pull Structure

Abstract

The present invention relates to the technical field of nucleic acid detection, and discloses an integrated nucleic acid detection device based on a flexible structure and a push-pull structure, which includes a detection main body, an upper cover, a bottom shell, and a pressing block disposed between the upper cover and the bottom shell; the detection main body is disposed in an accommodation cavity between the upper cover and the bottom shell; the detection main body includes a sample introduction tube, a nucleic acid extraction-free reagent chamber, a first buffer chamber, a nucleic acid amplification reagent chamber, a second buffer chamber, a mixing chamber, all of which are flexible reagent chambers, as well as a sample loading groove and a chromatographic test strip; the head end of the sample loading groove is connected to the mixing chamber, and the tail end of the sample loading groove is connected to the chromatographic test strip; the pressing block can move from one end of the upper cover to the other end, for squeezing the liquid in the detection main body to force the liquid to flow downstream. Since the liquid will only be squeezed and flow downstream when the pressing block is manually pushed to move, the controllability of the detection process can be realized.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid detection, and particularly to an integrated nucleic acid detection device based on a flexible structure and a push-pull structure. Background Art

[0002] Molecular diagnostic techniques play a very important role in pathogen detection, and nucleic acid detection is the most reliable and direct method among them. Conventional nucleic acid detection techniques mainly refer to fluorescence quantitative PCR, but this process must rely on large-scale instrument equipment and laboratory personnel with professional knowledge reserves, thus limiting its application in point-of-care nucleic acid detection.

[0003] In addition, in recent years, due to the expansion of detection requirements and the enrichment of detection scenarios, the rapid development of point-of-care nucleic acid detection has been promoted. Existing point-of-care nucleic acid detection devices will continue to run until the detection results are shown once the detection starts. However, during the detection process, the tester will inevitably be interrupted. When returning to the detection site, the detection results may no longer be accurate due to a long time interval.

[0004] Therefore, it is necessary to provide a new detection device to overcome the above defects. Summary of the Invention

[0005] The purpose of the present invention is to provide an integrated nucleic acid detection device based on a flexible structure and a push-pull structure with a controllable detection process.

[0006] To solve the above technical problems, the technical solution of the present invention is as follows:

[0007] An integrated nucleic acid detection device based on a flexible structure and a push-pull structure, comprising a detection main body, an upper cover, a bottom shell, and a pressing block installed between the upper cover and the bottom shell;

[0008] The detection main body is arranged in the accommodation cavity between the upper cover and the bottom shell; the detection main body includes a sample inlet tube, a nucleic acid extraction-free reagent chamber, a first buffer chamber, a nucleic acid amplification reagent chamber, a second buffer chamber, a mixing chamber, a sample loading slot, and a chromatographic test strip connected in sequence; the nucleic acid extraction-free reagent chamber, the first buffer chamber, the nucleic acid amplification reagent chamber, the second buffer chamber, and the mixing chamber are all flexible reagent chambers; the head end of the sample loading slot is connected to the mixing chamber, and the tail end of the sample loading slot is connected to the chromatographic test strip; wherein;

[0009] The pressing block can move from one end of the upper cover to the other end to squeeze the liquid in the detection main body, forcing the liquid to flow downstream.

[0010] Furthermore, two adjacent flexible reagent chambers are divided into two independent chambers by a weld seam, and one-third of the length of the weld seam is a virtual weld seam.

[0011] Furthermore, an upper sliding groove is provided on the upper cover, a partition wall is provided inside the bottom shell, the extrusion block includes a handle, a neck and an extrusion head connected in sequence, the handle protrudes from the top surface of the upper cover, the neck slides along the upper sliding groove, and the extrusion head is located between the partition wall and the long side wall of the bottom shell.

[0012] Furthermore, a side sliding groove is concavely provided on one side of the partition wall facing the extrusion head, and the extrusion head slides along the side sliding groove.

[0013] Furthermore, a clamping structure is concavely provided on the side wall of the upper sliding groove corresponding to the first buffer chamber, a limiting protrusion is provided on the neck, and the limiting protrusion cooperates with the clamping structure.

[0014] Furthermore, the detection device further includes a U-shaped buckle, the opening of the buckle faces the neck and is stuck on the neck, and the bottom surface of the buckle abuts against the top surface of the upper cover, so that the slider will not exert an extrusion effect on the liquid in the detection main body.

[0015] Furthermore, the sample loading groove is arranged parallel to the short side wall of the bottom shell, the chromatographic test strip is arranged parallel to the long side wall of the bottom shell, and a detection result display window is provided on the upper cover corresponding to the chromatographic test strip.

[0016] Furthermore, the first end of the sampling tube is stuck on a short side wall of the bottom shell and protrudes from the short side wall, or the first end of the sampling tube is stuck on the top surface of the upper cover and protrudes from the top surface of the upper cover.

[0017] Furthermore, the sampling tube is connected to the nucleic acid extraction-free reagent chamber through a rigid pipe and a flexible pipe in sequence, and the rigid pipe is inclined towards the bottom wall of the bottom shell.

[0018] Furthermore, two heating modules are further included, and the two heating modules are respectively used for heating the nucleic acid extraction-free reagent chamber and the nucleic acid amplification reagent chamber.

[0019] The technical solution of the present invention has the following advantages:

[0020] 1. The nucleic acid integrated detection device based on a flexible structure and a push-pull structure provided by the present invention includes a detection main body, an upper cover, a bottom shell, and a pressing block disposed between the upper cover and the bottom shell; the detection main body is disposed in a receiving cavity between the upper cover and the bottom shell; the detection main body includes a sampling tube, a nucleic acid extraction-free reagent chamber, a first buffer chamber, a nucleic acid amplification reagent chamber, a second buffer chamber, a mixing chamber, a loading groove, and a chromatographic test strip connected in sequence; the nucleic acid extraction-free reagent chamber, the first buffer chamber, the nucleic acid amplification reagent chamber, the second buffer chamber, and the mixing chamber are all flexible reagent chambers; the head end of the loading groove is connected to the mixing chamber, and the tail end of the loading groove is connected to the chromatographic test strip; wherein, the pressing block moves from one end of the upper cover to the other end for squeezing the liquid in the detection main body to force the liquid to flow downstream. In this way, only when the pressing block is manually pushed to move, the liquid will be squeezed to flow downstream, thereby realizing the controllability of the detection process.

[0021] 2. The nucleic acid integrated detection device based on a flexible structure and a push-pull structure provided by the present invention, the adjacent two flexible reagent chambers are divided into two independent chambers by a weld seam, and one-third of the length of the weld seam is a virtual weld seam. In this way, it can not only ensure the independence of the adjacent two flexible reagent chambers, but also smoothly connect the adjacent two flexible reagent chambers when the pressing block squeezes the flexible reagent chamber, thereby ensuring the smooth progress of the detection.

[0022] 3. The nucleic acid integrated detection device based on a flexible structure and a push-pull structure provided by the present invention, a clamping structure is recessed on the side wall of the upper chute corresponding to the first buffer chamber, and a limiting protrusion is provided on the neck of the pressing block. The limiting protrusion cooperates with the clamping structure to be used for prompting that the pressing block has slid in place, avoiding excessive movement of the pressing block, and further improving the controllability in the detection process.

[0023] 4. The nucleic acid integrated detection device based on a flexible structure and a push-pull structure provided by the present invention, the detection device further includes a U-shaped buckle, the opening of the buckle faces the neck and is stuck on the neck, and the bottom surface of the buckle abuts against the top surface of the upper cover, so that the slider will not exert a squeezing effect on the liquid in the detection main body. The setting of the buckle can prevent the pressing block from squeezing the detection main body unexpectedly and avoid the detection main body from being scrapped due to the accidental squeezing of the pressing block.

[0024] 5. The nucleic acid integrated detection device based on a flexible structure and a push-pull structure provided by the present invention, the loading groove is arranged parallel to the short side wall of the bottom shell, the chromatographic test strip is arranged parallel to the long side wall of the bottom shell, and a detection result display window is opened on the upper cover corresponding to the chromatographic test strip. In this way, it is beneficial to make full use of the size of the bottom shell and reduce the volume of the detection device. Description of the Drawings

[0025] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for the description of the specific embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0026] Figure 1 Schematic perspective view of the detection device in the embodiment of the present invention;

[0027] Figure 2 Schematic perspective view of the detection cylinder in the embodiment of the present invention;

[0028] Figure 3 Schematic perspective view of the upper cover in the embodiment of the present invention;

[0029] Figure 4 Schematic perspective view of the bottom shell in the embodiment of the present invention;

[0030] Figure 5 Schematic perspective view of the first angle of the extrusion block in the embodiment of the present invention;

[0031] Figure 6 Schematic perspective view of the second angle of the extrusion block in the embodiment of the present invention;

[0032] Figure 7 Schematic perspective view of the cooperation between the buckle and the extrusion block in the embodiment of the present invention;

[0033] Figure 8 Schematic perspective view of the buckle in the embodiment of the present invention;

[0034] Figure 9 Schematic diagram of the internal weld of the mixing chamber in the embodiment of the present invention.

[0035] Explanation of reference numerals:

[0036] 1. Detection main body; 11. Sealing cover; 12. Sampling tube; 121. Rigid pipeline; 122. Flexible pipeline; 13. Nucleic acid extraction-free reagent chamber; 14. First buffer chamber; 15. Nucleic acid amplification reagent chamber; 16. Second buffer chamber; 17. Mixing chamber; 18. Sampling groove; 178. Flexible bag tube; 171. Virtual weld; 172. Solid weld; 19. Chromatographic test strip.

[0037] 2. Upper cover; 21. Result display window; 22. Upper chute; 23. Positioning structure.

[0038] 3. Bottom case; 31. Partition wall; 32. Side chute; 33. First heating plate storage slot; 34. First battery storage slot; 35. First switch button; 36. Second heating plate storage slot; 37. Second battery storage slot; 38. Second switch button; 39. Test strip holder; 391. Test strip fixing piece; 310. Sampling tube limiting hole; 312. First display lamp; 313. Second display lamp;

[0039] 4. Extrusion block; 41. Handle; 42. Neck; 421. Limiting protrusion; 44. Extrusion head;

[0040] 5. Snap fastener. Detailed implementation manners

[0041] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. Apparently, the described embodiments are some but not all of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the scope of protection of the present invention.

[0042] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the present invention. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0043] In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, the terms "installation", "connection", and "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0044] In addition, the technical features involved in different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0045] Such as Figures 1 to 9As shown in the figure, the present invention provides a nucleic acid integrated detection device based on a flexible structure and a push-pull structure (hereinafter referred to as the detection device). The detection device mainly includes a detection main body 1, an upper cover 2, a bottom shell 3, a pressing block 4, and a buckle 5. The detection main body 1 is placed in the accommodation cavity between the bottom shell 3 and the upper cover 2.

[0046] The detection main body 1 includes a sampling tube 12, a nucleic acid extraction-free reagent chamber 13, a first buffer chamber 14, a nucleic acid amplification reagent chamber 15, a second buffer chamber 16, a mixing chamber 17, a sample loading groove 18, and a chromatographic test strip 19, which are connected in sequence. Since the nucleic acid extraction-free reagent chamber 13, the first buffer chamber 14, the nucleic acid amplification reagent chamber 15, the second buffer chamber 16, and the mixing chamber 17 are all chambers made of flexible materials, they are collectively referred to as flexible reagent chambers. The number of flexible reagent chambers can be determined according to the required reaction steps and functions.

[0047] In Figure 2 the perspective view, the sampling tube 12 is arranged perpendicular to the short side wall on the left side of the bottom shell 3 for injecting samples. The outer end of the sampling tube 12 is threadedly connected to a sealing cap 11 and sealed by the sealing cap 11. As Figure 2 shown, one end of the sampling tube 12 located inside the bottom shell 3 is connected to a rigid pipe 121 that slopes downward towards the bottom of the bottom shell 3 to reduce the residue of the reagent in the rigid pipe 121. One end of the rigid pipe 121 far from the sampling tube 12 is connected to a section of flexible pipe 122 so that the pressing block 4 can squeeze the sample into the flexible reagent chamber. The pipeline connected to one end of the sampling tube 12 located inside the bottom shell 3 can also be a soft pipeline. In this case, the length of the soft pipeline is relatively large, and it can be used for liquid samples as well as swab samples, and the sample loss can be reduced by squeezing the soft pipeline with a swab. Before sample addition, the sampling tube 12 can be placed in a vertically upward state by changing the placement orientation of the entire device. In this way, not only is it convenient for sample addition, but it also helps the sample to smoothly enter the flexible reagent chamber.

[0048] The buffer solution in the first buffer chamber 14 is the buffer in the corresponding nucleic acid amplification system in the nucleic acid amplification reagent chamber 15. The nucleic acid amplification reagent chamber 15 can use isothermal amplification technologies such as RPA and LAMP, or PCR variable-temperature amplification technology. The volume of the nucleic acid amplification reagent chamber 15 is twice the volume of the liquid therein, so as to make the nucleic acid amplification system as flat as possible, increase the contact area with the following heating plate, and make the liquid heat evenly. The reagent in the second buffer chamber 16 can be water or other diluents, which are used to dilute the amplification product before reaching the chromatographic test strip 19 for detection.

[0049] When mixing the reagents in each flexible reagent chamber, the entire device can be inverted 2 - 3 times up and down to obtain a better mixing effect. The size of each flexible reagent chamber can be adjusted according to the volume of different reaction reagents, but it is necessary to ensure the unified length of the weld between two adjacent flexible reagent chambers, and the width of the weld needs to be reserved to avoid affecting the volume of each flexible reagent chamber. 1 / 3 of the length of each weld between two adjacent flexible reagent chambers is welded by the technique of false soldering, and the remaining 2 / 3 of the length is welded by the ordinary thermal welding technique. It should be noted that the liquid pressure in the previous flexible reagent chamber can be increased to flush open the part of the false soldered weld located downstream, so as to realize the mixing of the liquid in two adjacent flexible reagent chambers. During this process, the solid soldered weld will not be affected. The flexible reagent chamber is made of flexible materials, and materials such as polypropylene, polyethylene, polyurethane, and polyolefin copolymers can be selected.

[0050] In Figure 2 From this perspective, the head end of the sample loading groove 18 is connected to the mixing chamber 17 through a flexible bag tube 178 (the flexible bag tube 178 is essentially an extension of the mixing chamber 17), and a chromatographic test strip 19 for detection is provided at the tail end of the sample loading groove 18. In Figure 1 From this perspective, the sample loading groove 18 is arranged parallel to the short side wall on the right side of the bottom shell 3. The chromatographic test strip 19 is arranged parallel to the long side wall of the bottom shell 3. The lower surface of the sample loading groove 18 is fixed to the inner bottom surface of the bottom shell 3 by double-sided tape to prevent it from shaking during the transportation of the detection main body 1. The detection result is visually presented through the chromatographic test strip 19, and multiplex detection of the same system can be realized by using different primer probes for labeling.

[0051] On the upper cover 2, there are a result display window 21, an upper chute 22 for the neck 42 of the following extrusion block 4 to slide, and a clamping structure 23 that cooperates with the limit protrusion 421 on the extrusion block 4 (in this embodiment, the clamping structure 23 is a circular groove, and in other embodiments, it can also be a long groove). The clamping structure 23 corresponds to the first buffer chamber 14. The width of the upper chute 22 is the same as the width dimension of the neck 42 to prevent the extrusion block 4 from shaking left and right during the sliding process. When the limit protrusion 421 on the extrusion block 4 enters the preset clamping structure 23, it can remind the operator that the extrusion block 4 has slid to the preset position and there is no need to push the extrusion block 4 to move further to the right to control the reaction process.

[0052] Inside the bottom shell 3, there is a partition wall 31. On the side of the partition wall 31 facing the extrusion head 44, there is an inwardly concave side chute 32. The extrusion head 44 of the following extrusion block 4 slides along the side chute 32 between the partition wall 31 and a long side wall of the bottom shell 3. The side chute 32 is in an "L" shape, and its height is the same as the height of the extrusion head 44 of the extrusion block 4.

[0053] Inside the bottom case 3, there are a first heating plate storage slot 33 for storing the first heating plate, a first battery storage slot 34 for supplying power to the first heating plate, a first switch button 35, and a first display light 312. Among them, the first heating plate storage slot 33 is arranged directly below the nucleic acid extraction-free reagent chamber 13.

[0054] Inside the bottom case 3, there are also a second heating plate storage slot 36 for storing the second heating plate, a second battery storage slot 37 for supplying power to the second heating plate, a second switch button 38, and a second display light 313. Among them, the second heating plate storage slot 36 is arranged directly below the nucleic acid amplification reagent chamber 15.

[0055] Of course, the aforementioned heating plate can also be replaced by a warming patch or a self-heating pack. For the convenience of description, the heating plate, warming patch, and self-heating pack are collectively referred to as the heating module. When the heating module is a warming patch or a self-heating pack, there is no need to set up a battery anymore. In addition, whether to set up the heating module depends on whether the reaction system has a heating requirement. When the heating module is not set up, the first heating plate storage slot 33, the second heating plate storage slot 36, the first battery storage slot 34, and the second battery storage slot 37 can be retained. Of course, when the reaction system has no heating requirement, the first heating plate storage slot 33, the second heating plate storage slot 36, the first battery storage slot 34, and the second battery storage slot 37 may not be set up either.

[0056] At the bottom of the bottom case 3, there is also a test strip holder 39. On the test strip holder 39, there are multiple test strip fixing parts 391 for fixing the chromatographic test strip 19 to prevent it from shifting during transportation or use.

[0057] On the short side wall of the bottom case 3 adjacent to the sampling tube 12, there is a sampling tube limiting hole 310 for fixing the sampling tube 12 of the detection main body 1. The inner diameter of the sampling tube limiting hole 310 is equivalent to the outer diameter of the sampling tube 12.

[0058] As Figure 5 and Figure 6 shown, as an implementation manner, the extrusion block 4 includes a handle 41, a neck 42, and an extrusion head 44 that are connected in sequence. The handle 41 can be of any shape and always protrudes from the top surface of the upper cover 2. On one side of the neck 42, there is a limiting protrusion 421, and the limiting protrusion 421 cooperates with the following clamping structure 23. The extrusion head 44 is used to squeeze the flexible reagent chamber to communicate the upstream flexible reagent chamber with the downstream flexible reagent chamber. Specifically, the extrusion head 44 is a thin wedge-shaped head, in the shape of a straight line. In the width direction of the flexible reagent chamber, the width of the extrusion head 44 at least completely covers the flexible reagent chamber, and the width value of the extrusion head 44 is equivalent to the spacing value between the following side chute 32 and the long side wall of the corresponding bottom case 3, so as to ensure that when the extrusion block 4 slides, the liquid in the corresponding flexible reagent chamber can be driven out completely and all enter the downstream flexible reagent chamber without any position deviation.

[0059] like Figure 7 and Figure 8 As shown, the buckle 5 is U-shaped, and the opening of the buckle 5 faces the neck 42. The width of the buckle 5 is greater than the width of the upper slide 22, and less than the width of the handle 41, so that the buckle 5 can be stuck on the neck 42 of the extrusion block 4 without detaching from the extrusion block 4 upward. During transportation or before detection, the bottom surface of the buckle 5 is against the top surface of the upper cover 2, so that the extrusion head 44 of the extrusion block 4 is not in contact with the flexible pipe 122 and the flexible reagent chamber below, or although it is in contact, the extrusion force is small. When the extrusion head 44 is needed to extrude the flexible pipe 122 and the flexible reagent chamber, remove the buckle 5, press the extrusion block 4 downward to the bottom of the side slide 32, and slide the extrusion block 4. The first heating plate and the second heating plate are collectively referred to as heating plates.

[0060] like Figure 9 As shown, virtual welds 171 are respectively provided at both ends of the mixing chamber 17. The virtual welds 171 will only open under the extrusion of the extrusion block 4. A plurality of real welds 172 are alternately arranged between the two virtual welds 171 to form a Z-shaped mixing flow channel in the mixing chamber 17 to enhance the mixing effect.

[0061] The specific operation process of the nucleic acid integrated detection device based on the flexible structure and push-pull structure in this embodiment is as follows:

[0062] Stand the detection device upright, make the sample injection tube 12 point vertically upward, add the sample, tighten the sealing cover 11, and then the sample flows into the nucleic acid extraction-free reagent chamber 13 and mixes with the nucleic acid extraction-free reagent;

[0063] Remove the buckle 5 and press the extrusion block 4 toward the bottom of the bottom shell 3 until the bottom of the extrusion head 44 abuts against the flexible pipe 122 to squeeze the sample in the flexible pipe 122 into the nucleic acid extraction-free reagent chamber 13;

[0064] The detection device is placed horizontally. Due to the obstruction of the squeezing block 4 , the mixed liquid in the nucleic acid-free extraction reagent chamber 13 will not flow back toward the injection tube 12 .

[0065] Turn on the first switch button 35, the first display light 312 lights up, the first heating plate heats the nucleic acid extraction-free reagent chamber 13 to a preset temperature, and the sample begins to lyse until the first display light 312 goes out;

[0066] Hold the handle 41 and push the extrusion block 4 in the direction away from the sampling tube 12. During the sliding process of the extrusion block 4, the lysed solution in the nucleic acid extraction-free reagent chamber 13 and the RPA buffer solution in the first buffer chamber 14 enter the nucleic acid amplification reagent chamber 15 and are mixed with the RPA reagent in the nucleic acid amplification reagent chamber 15. When the limit protrusion 421 on the extrusion block 4 coincides with the clamping structure 23 and a jamming feeling appears, stop sliding the extrusion block 4;

[0067] Open the second switch button 38, the second display light 313 lights up, and the second heating plate heats the nucleic acid amplification reagent chamber 15. The sample undergoes an amplification reaction in the nucleic acid amplification reagent chamber 15 until the second display light 313 goes out, and the amplification is completed to obtain the amplification product;

[0068] Hold the handle 41 and continue to push the extrusion block 4 in the direction away from the sampling tube 12. The amplified product flows through the second buffer chamber 16 and is mixed and diluted with the diluent in the second buffer chamber 16, then enters the mixing chamber 17 for mixing, and finally reaches the chromatography test strip 19 via the flexible bag tube 178 and the sample loading groove 18 for detection, and the detection result is output by the result display window 21.

[0069] The nucleic acid integrated detection device based on the flexible structure and the push-pull structure provided by the present invention adopts unique chamber isolation and fluid control technologies, realizing rapid, simple, and low-cost nucleic acid detection. Specifically, the device constructs different functional chambers and mixing channels in the detection main body 1 by using solid welds and virtual welds. The solid welds can ensure the sealing and isolation between chambers, while the virtual welds provide a controllable propulsion connection mechanism, allowing the virtual welds to be forced to open by physical means (by the extrusion of the extrusion block 4) only when needed to achieve the connection between adjacent chambers.

[0070] In addition, the nucleic acid integrated detection device based on the flexible structure and the push-pull structure provided by the present invention adopts a slider extrusion mechanism (in this embodiment, the physical extrusion of the chamber by the extrusion block 4 promotes the liquid flow), so as to control the reaction process. The advantage of this design lies in its simplicity and directness, without relying on external pumping systems or complex valve controls. The implementation of the slider extrusion mechanism promotes the continuous flow of liquid in the microchannels and between chambers by applying mechanical force to specific areas of the flexible reagent chamber, ensuring the uniform mixing and rapid transfer of reactants. This design not only simplifies the fluid control mechanism, reduces the manufacturing cost and operation difficulty of the device, but also expands the use environment of the detection system, making it easier to be used in resource-limited areas or on-site rapid detection scenarios.

[0071] In addition, the design of the nucleic acid integrated detection device based on the flexible structure and the push-pull structure provided by the present invention fully considers the user experience and operational simplicity, enabling non-professionals to easily perform nucleic acid detection operations. Moreover, due to its small size and good portability, it can be applied to a variety of application scenarios (including clinical laboratories, on-site detection, home self-detection, etc.), greatly expanding the accessibility and application scope of nucleic acid detection.

[0072] Obviously, the above embodiments are merely examples given for clear illustration and are not limitations on the implementation manners. For those of ordinary skill in the art, other different forms of changes or alterations can be made based on the above description. It is not necessary and impossible to enumerate all the implementation manners here. And the obvious changes or alterations derived therefrom still fall within the protection scope of the present invention.

Claims

1. A nucleic acid integrated detection device based on a flexible structure and a push-pull structure, characterized in that: It comprises a detection body (1), an upper cover (2), a bottom shell (3), and an extrusion block (4) installed between the upper cover (2) and the bottom shell (3); The detection body (1) is arranged in a containing cavity between the upper cover (2) and the bottom shell (3); the detection body (1) comprises an injection tube (12), a nucleic acid extraction-free reagent chamber (13), a first buffer chamber (14), a nucleic acid amplification reagent chamber (15), a second buffer chamber (16), a mixing chamber (17), a sample loading groove (18) and a chromatography test paper (19) which are connected in sequence; the nucleic acid extraction-free reagent chamber (13), the first buffer chamber (14), the nucleic acid amplification reagent chamber (15), the second buffer chamber (16) and the mixing chamber (17) are all flexible reagent chambers; the head end of the sample loading groove (18) is connected to the mixing chamber (17), and the tail end of the sample loading groove (18) is connected to the chromatography test paper (19); wherein; The squeezing block (4) can move from one end of the upper cover (2) to the other end, and is used to squeeze the liquid in the detection body (1) to force the liquid to flow downstream; The upper cover (2) is provided with an upper slide groove (22), the bottom shell (3) is provided with a partition wall (31), the extrusion block (4) comprises a handle (41), a neck (42) and an extrusion head (44) which are connected in sequence, the handle (41) protrudes from the top surface of the upper cover (2), the neck (42) can slide along the upper slide groove (22), and the extrusion head (44) is located between the partition wall (31) and the long side wall of the bottom shell (3); Two adjacent flexible reagent chambers are divided into two independent chambers by a weld, and 1 / 3 of the length of each weld between two adjacent flexible reagent chambers is welded by virtual welding technology, and the remaining 2 / 3 of the length is welded by ordinary thermal welding technology.

2. The nucleic acid integrated detection device based on flexible structure and push-pull structure according to claim 1 is characterized in that: A side sliding groove (32) is provided on the partition wall (31) on one side facing the extrusion head (44) and is recessed inwardly, and the extrusion head (44) slides along the side sliding groove (32).

3. The nucleic acid integrated detection device based on flexible structure and push-pull structure according to claim 1 is characterized in that: A locking structure (23) is recessed on the side wall of the upper slide groove (22) corresponding to the first buffer chamber (14), and a limiting protrusion (421) is provided on the neck (42), and the limiting protrusion (421) is engaged with the locking structure (23).

4. The nucleic acid integrated detection device based on flexible structure and push-pull structure according to claim 1, characterized in that: The detection device further comprises a U-shaped buckle (5), the opening of the buckle (5) faces the neck (42) and is clamped on the neck (42), and the bottom surface of the buckle (5) abuts against the top surface of the upper cover (2), so that the squeezing block (4) does not exert a squeezing effect on the liquid in the detection body (1).

5. The nucleic acid integrated detection device based on a flexible structure and a push-pull structure according to any one of claims 1 to 4, characterized in that: The sample loading groove (18) is arranged parallel to the short side wall of the bottom shell (3), the chromatography test paper (19) is arranged parallel to the long side wall of the bottom shell (3), and a test result display window (21) is provided on the upper cover (2) corresponding to the chromatography test paper (19).

6. The nucleic acid integrated detection device based on flexible structure and push-pull structure according to claim 5, characterized in that: The head end of the sample injection tube (12) is clamped on a short side wall of the bottom shell (3) and protrudes from the short side wall, or the head end of the sample injection tube (12) is clamped on the top surface of the upper cover (2) and protrudes from the top surface of the upper cover (2).

7. The nucleic acid integrated detection device based on flexible structure and push-pull structure according to claim 6, characterized in that: The sample injection tube (12) is connected to the nucleic acid extraction-free reagent chamber (13) via a rigid pipe (121) and a flexible pipe (122) in sequence, and the rigid pipe (121) is arranged to be inclined toward the bottom wall of the bottom shell (3).

8. The nucleic acid integrated detection device based on flexible structure and push-pull structure according to claim 1, characterized in that: It also includes two heating modules, which are used to heat the nucleic acid-free extraction reagent chamber (13) and the nucleic acid amplification reagent chamber (15) respectively.

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