Two-photon microscope imaging fixing device

Abstract

The invention provides a two-photon microscope imaging fixing device, and relates to the technical field of medical examination equipment. The two-photon microscope imaging fixing device comprises a rack, a claw part unfolding and fixing mechanism and an imaging piece, wherein the rack is provided with an object placing plate for placing an animal to be observed; the claw unfolding and fixing mechanism comprises a claw unfolding assembly and an adjusting assembly, the claw unfolding assembly comprises a supporting plate and an observation slide, the supporting plate is arranged on the storage plate, and the observation slide is movably arranged on the supporting plate through the adjusting assembly and is matched with the supporting plate to form a size-adjustable clamping space; the support plate and the observation slide can clamp a claw part of an animal to be observed and enable the claw part to keep an unfolded state; and the imaging piece is arranged above the observation slide and is used for observing and shooting the claw part unfolded in the clamping space. The two-photon microscope imaging fixing device can realize tiling and fixing of animal claws, thereby improving observation convenience and accuracy of observation results.

Description

Technical Field

[0001] The invention relates to the technical field of medical testing equipment, and in particular to a two-photon microscope imaging fixing device. Background Art

[0002] In the field of medicine, it is often necessary to observe the blood circulation of the soles of mice in the study of vascular function. Existing instruments for detecting the blood circulation status of the soles of mice, such as pain meters and mouse toe swelling measurement equipment, only indirectly help evaluate the blood circulation status of the soles of the mice through other indicators, and cannot directly observe and evaluate the soles of the mice; and most of the detection devices using two-photon microscopes fix the head of the animal. When the head of the animal is fixed, the animal lies prone with its paws curled up, and it is impossible to image the blood vessels in the paws; therefore, when observing and evaluating the soles of the animals, the operation is more troublesome and the accuracy of the observation results is low. Summary of the invention

[0003] The object of the present invention is to provide a two-photon microscope imaging fixing device, which can realize the flat fixation of animal claws, thereby improving the convenience of observation and the accuracy of observation results.

[0004] To achieve this object, the present invention adopts the following technical solutions:

[0005] Two-photon microscope imaging fixture, including:

[0006] A rack, provided with a storage board for placing the animals to be observed;

[0007] A claw deployment fixing mechanism, comprising a claw deployment component and an adjustment component, wherein the claw deployment component comprises a support plate and an observation glass, wherein the support plate is arranged on the storage plate, and the observation glass is movably arranged on the support plate through the adjustment component and cooperates with the support plate to form a clamping space with adjustable size, wherein the support plate and the observation glass can clamp the claw of the animal to be observed and keep the claw in an deployed state;

[0008] An imaging component is arranged above the observation glass slide, and is used to observe and photograph the claw portion unfolded in the clamping space.

[0009] As a further technical solution, the adjustment assembly includes an adjustment screw, which is fixedly connected to the upper end surface of the support plate and extends along the Z direction. The observation slide is movably connected to the adjustment screw along the Z direction through an adjustment hole.

[0010] As a further technical solution, two adjusting screws are arranged near the edge of the support plate, the adjusting holes are arranged corresponding to the adjusting screws, and the two adjusting screws are arranged symmetrically about the center of the support plate.

[0011] As a further technical solution, the claw deployment and fixing mechanism also includes a connecting assembly, which includes a connecting rod, and a plurality of connecting holes are spaced apart on the storage plate. The support plate is connected to the connecting rod, and the connecting rod can be detachably connected to any of the connecting holes.

[0012] As a further technical solution, a plurality of the connection holes are arranged in a rectangular array on the storage plate, and each of the connection holes is arranged as a long hole extending along the Y direction.

[0013] As a further technical solution, the connecting rod is configured as a connecting screw, the connecting assembly further comprises a connecting nut, the support plate is movably connected to the connecting screw through a connecting hole, the connecting nut is threadedly connected to the connecting screw, and can adjust the relative position of the support plate along the Z direction;

[0014] Alternatively, the connecting rod is configured as a telescopic rod, and the support plate is fixedly connected to the connecting rod.

[0015] As a further technical solution, the rack further includes a bottom plate and a rack connecting rod, and the storage plate is arranged above the bottom plate through the rack connecting rod.

[0016] As a further technical solution, the storage plate is provided with an auxiliary hole, through which the storage plate is movably connected to one end of the frame connecting rod away from the bottom plate, and the auxiliary hole is configured as a long strip hole extending along the X direction.

[0017] As a further technical solution, the frame connecting rod is configured as a threaded connecting rod or a telescopic connecting rod.

[0018] As a further technical solution, the two-photon microscope imaging fixture further includes an anti-vibration platform, and the frame is fixedly connected to the upper end surface of the anti-vibration platform.

[0019] Compared with the prior art, the two-photon microscope imaging fixing device provided by the present invention has the following technical advantages:

[0020] Since the two-photon microscope imaging fixture is provided with a claw expansion component and an imaging element, and the support plate and the observation glass slide in the claw expansion component can cooperate with each other to form a clamping space with adjustable size; therefore, when it is necessary to observe the sole of the animal, the animal to be observed is first placed on its back on the placement plate, and then the adjustment component is adjusted to make the support plate and the observation glass slide move away from each other to increase the clamping space; then the claw of the animal to be observed is placed in the clamping space, and at this time the claw is expanded so that the sole of the animal to be observed faces the observation glass slide; then the adjustment component is adjusted to make the support plate and the observation glass slide approach each other, so that the observation glass slide and the support plate cooperate with each other to clamp the expanded claw, ensuring that the claw is always clamped in the clamping space, and at the same time ensuring that the claw in the clamping space is always in an expanded state under the clamping action; finally, water is dripped on the observation glass slide or transparent ophthalmic gel is used, and imaging can be performed through the imaging element. The entire process can be achieved with the help of a support plate and an observation slide, with a simple structure and easy operation; at the same time, during the observation and evaluation process, the animal's claws can be kept unfolded at all times to ensure the accuracy of the observation and evaluation results. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments of the present invention. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the contents of the embodiments of the present invention and these drawings without paying any creative work.

[0022] Figure 1 is a schematic structural diagram of a two-photon microscope imaging fixing device provided in an embodiment of the present invention;

[0023] Figure 2 yes Figure 1 A partial enlarged view of the middle A;

[0024] Figure 3 It is a structural schematic diagram of another implementation manner of the two-photon microscope imaging fixing device provided in an embodiment of the present invention.

[0025] In the figure:

[0026] 100, rack; 110, storage plate; 111, auxiliary hole; 120, bottom plate; 130, rack connecting rod; 140, connecting rod nut;

[0027] 200, claw deployment fixing mechanism; 210, claw deployment assembly; 211, support plate; 212, observation slide; 220, adjustment assembly; 221, adjustment screw; 222, adjustment nut; 230, connection assembly; 231, connection rod; 232, connection hole; 233, connection nut;

[0028] 300, imaging parts;

[0029] 400. Anti-vibration table. DETAILED DESCRIPTION

[0030] Before any embodiments of the application are explained in detail, it is to be understood that the application is not limited in its application to the details of construction and the arrangement of components set forth in the following description or illustrated in the above drawings.

[0031] In this application, the terms "comprises", "includes", "has" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device that includes a series of elements includes not only those elements, but also includes other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of more restrictions, an element defined by the sentence "comprises a ..." does not exclude the presence of other identical elements in the process, method, article or device that includes the element.

[0032] In this application, the term "and / or" is a description of the association relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. In addition, the character " / " in this application generally indicates that the related objects before and after are in an "and / or" relationship.

[0033] In the present application, the terms "connect", "combine", "couple", and "install" may refer to direct connection, combination, coupling, or installation, or indirect connection, combination, coupling, or installation. For example, direct connection refers to two parts or components being connected together without the need for an intermediary, and indirect connection refers to two parts or components being connected to at least one intermediary respectively, and the two parts or components being connected via the intermediary. In addition, "connect" and "couple" are not limited to physical or mechanical connections or couplings, and may include electrical connections or couplings.

[0034] In the present application, a person of ordinary skill in the art will understand that relative terms (e.g., "about," "approximately," "substantially," etc.) used in conjunction with quantities or conditions include the values ​​and have the meaning indicated by the context. For example, the relative terms include at least the degree of error associated with the measurement of a specific value, the tolerance caused by manufacturing, assembly, use, etc. associated with a specific value. Such terms should also be considered to disclose the addition or subtraction of 5%, 10% or more from both ends. Numerical values ​​that do not adopt relative terms should also be disclosed as specific values ​​with tolerances. In addition, "substantially" when expressing a relative angular position relationship (e.g., substantially parallel, substantially perpendicular) may refer to adding or subtracting a certain degree (e.g., 1 degree, 5 degrees, 10 degrees or more) based on the indicated angle.

[0035] In this application, it will be understood by those skilled in the art that the function performed by a component can be performed by one component, multiple components, one part, or multiple parts. Similarly, the function performed by a part can also be performed by one part, one component, or a combination of multiple parts.

[0036] In the present application, the terms "upper", "lower", "left", "right", "front", "back" and other directional words are described based on the orientation and positional relationship shown in the accompanying drawings, and should not be understood as limiting the embodiments of the present application. In addition, in the context, it is also necessary to understand that when it is mentioned that an element is connected to another element "upper" or "lower", it can not only be directly connected to another element "upper" or "lower", but also indirectly connected to another element "upper" or "lower" through an intermediate element. It should also be understood that directional words such as upper side, lower side, left side, right side, front side, back side, etc. not only represent the positive orientation, but can also be understood as the lateral orientation. For example, the bottom can include directly below, lower left, lower right, lower front, and lower back, etc.

[0037] Combination Figures 1 to 3 As shown, the two-photon microscope imaging and fixing device provided in this embodiment is used to photograph and observe the paws of the animal to be observed after they are flattened and fixed, thereby improving the convenience of observation and the accuracy of observation results. In this embodiment, the animal to be observed takes a live anesthetized mouse as an example. Specifically, the two-photon microscope imaging fixing device includes a frame 100, a claw unfolding fixing mechanism 200 and an imaging element 300. The frame 100 is provided with a placement plate 110 for placing the live anesthetized mouse; the claw unfolding fixing mechanism 200 includes a claw unfolding component 210 and an adjustment component 220. The claw unfolding component 210 includes a support plate 211 and an observation slide 212. The support plate 211 is arranged on the placement plate 110. The observation slide 212 is movably arranged on the support plate 211 through the adjustment component 220, and cooperates with the support plate 211 to form a clamping space with adjustable size. The clamping space can clamp the claw of the live anesthetized mouse and unfold the claw; the imaging element 300 is arranged above the observation slide 212, and the imaging element 300 is used to observe and shoot the claw unfolded in the clamping space.

[0038] Since the two-photon microscope imaging fixture is provided with a claw deployment component 210 and an imaging member 300, and the support plate 211 and the observation slide 212 in the claw deployment component 210 can cooperate with each other to form a clamping space with adjustable size; therefore, when it is necessary to observe the sole of the animal, the living anesthetized mouse is first placed on its back on the placement plate 110, and then the adjustment component 220 is adjusted to make the support plate 211 and the observation slide 212 move away from each other to increase the clamping space; then the claw of the living anesthetized mouse is placed in the clamping space, and the clamping space is adjusted accordingly. The claw is unfolded when the observation slide 212 is opened, so that the sole of the living anesthetized mouse faces the observation slide 212; the adjustment component 220 is then adjusted to bring the support plate 211 and the observation slide 212 closer to each other, so that the observation slide 212 and the support plate 211 cooperate with each other to clamp the unfolded claw, ensuring that the claw is always clamped in the clamping space, and at the same time ensuring that the claw in the clamping space is always in an unfolded state under the clamping action; finally, water is dripped on the observation slide 212 or transparent ophthalmic gel is used, and imaging can be performed through the imaging element 300. The whole process can be realized with the help of the support plate 211 and the observation slide 212, with a simple structure and convenient operation; at the same time, the claw of the animal can be always unfolded during the observation and evaluation process to ensure the accuracy of the observation and evaluation results.

[0039] Since the claw deployment fixing mechanism 200 in this embodiment is based on a two-photon microscope, the imaging element 300 in this embodiment is set as a two-photon microscope lens; in other embodiments, according to actual needs, the imaging element 300 can be set as a camera, etc. In order to effectively ensure the imaging effect, another plate that cooperates with the support plate 211 to form a clamping space is set as an observation slide 212. In other embodiments, according to actual needs, the observation slide 212 can be set as a transparent plate such as an acrylic plate.

[0040] Furthermore, the two-photon microscope imaging fixing device also includes an anti-vibration table 400, and the frame 100 is fixedly connected to the upper end surface of the anti-vibration table 400. In this way, during the fixation and observation of live anesthetized mice, the anti-vibration table 400 reduces the impact of external components on the frame 100, thereby reducing the probability of the frame 100 vibrating or shifting due to external forces, thereby further improving the accuracy of observation, evaluation and other results and the convenience of operation. The specific structure of the anti-vibration table 400 can be set with reference to the prior art, and will not be repeated here.

[0041] Preferably, the adjustment assembly 220 includes an adjustment screw 221 and an adjustment nut 222. The adjustment screw 221 is fixedly connected to the upper end surface of the support plate 211 and extends along the Z direction. The observation slide 212 is movably connected to the adjustment screw 221 through an adjustment hole. The adjustment nut 222 is threadedly connected to the adjustment screw 221 and can adjust the relative position of the observation slide 212 along the Z direction.

[0042] Specifically, in this embodiment, two adjusting nuts 222 are provided on the adjusting screw 221, and the observation glass 212 is movably connected to the adjusting screw 221 through the adjusting hole and is located between the two adjusting nuts 222. When the claw portion needs to be placed in the clamping space, the two adjusting nuts 222 are screwed so that the two adjusting nuts 222 drive the observation glass 212 to rise in the Z direction, thereby increasing the spatial volume of the clamping space, so as to facilitate placing the claw portion in the clamping space and unfolding the claw portion; then, the two adjusting nuts 222 are screwed in the opposite direction so that the two adjusting nuts 222 drive the observation glass 212 to descend in the Z direction until the observation glass 212 abuts against the unfolded claw portion, thereby achieving the fixing and state maintenance of the claw portion. During the whole process, the placement and positioning of the claw can be achieved by twisting the two adjusting nuts 222, and the structure is simple and easy to operate; at the same time, the upper and lower adjusting nuts 222 can adjust the position of the observation glass slide 212. After the position of the observation glass slide 212 is adjusted, the observation glass slide 212 is prevented from shifting in the Z direction, thereby ensuring the clamping stability of the claw in the clamping space.

[0043] In some other embodiments, the adjustment hole is set as a threaded hole, and the adjustment screw 221 is directly threadedly matched with the corresponding threaded hole. When adjusting the position of the observation glass slide 212, the observation glass slide 212 can be rotated around the adjustment screw 221.

[0044] The number of the adjusting screws 221 can be appropriately increased or decreased according to actual needs, for example, the adjusting screws 221 can be set to one, three, five, etc., and the number of the adjusting nuts 222 is set corresponding to the adjusting screws 221. In this embodiment, two adjusting screws 221 are provided. Specifically, two adjusting screws 221 are provided near the edge of the support plate 211, the adjusting nuts 222 and the adjusting holes are provided corresponding to the adjusting screws 221, and the two adjusting screws 221 are symmetrically provided about the center of the support plate 211.

[0045] Specific combination Figure 2 As shown, with such arrangement, when the two adjusting nuts 222 drive the observation glass slide 212 to descend along the Z direction and make the observation glass slide 212 abut against the unfolded claws, the two symmetrically arranged adjusting screws 221 cooperate with each other to ensure that the observation glass slide 212 has the same tightening effect on the unfolded claws, thereby avoiding the partial curling or displacement of the clamped claws, thereby further improving the accuracy of observation, evaluation and other results; at the same time, when the claws are placed in the clamping space, interference between the adjusting screws 221 and the claws can be avoided as much as possible, thereby improving the convenience of operation.

[0046] In some other embodiments, the adjustment component 220 only includes a telescopic adjustment rod, and the support plate 211 and the observation glass 212 are both fixedly connected to the telescopic screw. The telescopic state of the telescopic screw itself is used to change the distance between the support plate 211 and the observation glass 212, so as to achieve the placement of the claw and the clamping and positioning of the claw. Alternatively, the adjustment component 220 only includes an adjustment tension spring, and the two ends of the adjustment tension spring are respectively fixedly connected to the two end surfaces close to each other on the support plate 211 and the observation glass 212; when the claw needs to be placed in the clamping space, the observation glass 212 is moved along the Y direction, at this time, the volume of the clamping space becomes larger, and the adjustment tension spring is stretched; the claw is placed in the clamping space to drive the observation glass 212 to move in the direction close to the support plate 211 until the observation glass 212 abuts against the unfolded claw; in this state, under the effect of the adjustment tension spring itself, the observation glass 212 cooperates with the support plate 211 to achieve the fixation and state maintenance of the claw in the clamping space. When the adjustment component 220 only includes a telescopic adjustment rod or an adjustment tension spring, the number of the telescopic adjustment rod or the adjustment tension spring is set according to actual needs, and this application does not make any specific limitations.

[0047] Preferably, in order to improve the applicability of the device, the claw deployment fixing mechanism 200 also includes a connecting assembly 230, the connecting assembly 230 includes a connecting rod 231 and a plurality of connecting holes 232, the plurality of connecting holes 232 are arranged at intervals on the storage plate 110, the support plate 211 is connected to the connecting rod 231, and the connecting rod 231 is detachably connected to any connecting hole 232. According to the size of the live anesthetized mouse or the position of the claw to be observed, the connecting rod 231 is connected to the corresponding connecting hole 232, so as to adjust the area and position of the storage area on the storage plate 110, so as to place the target live anesthetized mouse in the storage area, and fix and maintain its claw. For example, when the target animal to be observed is large in size, the connecting rod 231 is connected to the connecting hole 232 near the edge of the storage plate 110, so as to increase the storage area on the storage plate 110 and facilitate the target animal to be completely placed in the storage area.

[0048] Furthermore, a plurality of connection holes 232 are arranged in a rectangular array on the storage plate 110, and each connection hole 232 is arranged as a long hole extending along the Y direction. With such arrangement, after the connection rod 231 is connected to the target connection hole 232, the relative position of the connection rod 231 and the target connection hole 232 can be changed in the Y direction according to the size and position of the target animal in the storage area, thereby completing the position fine-tuning of the claw deployment fixing mechanism 200 in the Y direction, so as to further enhance the fixing and state maintenance effect of the claw of the target animal. The number of connection holes 232 can be set according to actual needs. In this embodiment, six connection holes 232 arranged in a rectangular array are provided on the storage plate 110, as shown in FIG. Figure 1 and Figure 3shown.

[0049] Furthermore, the connecting rod 231 is configured as a connecting screw, and the connecting assembly 230 also includes a connecting nut 233. The support plate 211 is movably connected to the connecting screw through the connecting hole 232. The connecting nut 233 is threadedly connected to the connecting screw and can adjust the relative position of the support plate 211 along the Z direction.

[0050] Specifically, in this embodiment, two connecting nuts 233 are provided on the connecting screw. After the connecting screw is connected to the storage plate 110, the two connecting nuts 233 are respectively located on both sides of the storage plate 110, and the support plate 211 is connected to the connecting screw. When it is necessary to change the height of the clamping space according to the target claw to fix and maintain the claws of animals of different sizes, the two connecting nuts 233 are screwed at the same time to change the relative position of the connecting screw and the storage plate 110. At the same time, when it is necessary to change the position and size of the storage area, the connecting nut 233 located below the storage plate 110 is removed from the connecting screw, and the connecting screw can be removed from the storage plate 110. The structure is simple and the operation is convenient.

[0051] In some other embodiments, the connecting rod 231 is configured as a telescopic rod, and the support plate 211 is fixedly connected to the connecting rod 231 . By changing the telescopic amount of the connecting rod 231 , the height of the clamping space is changed.

[0052] Furthermore, the rack 100 further includes a bottom plate 120 and a rack connecting rod 130 , and the storage plate 110 is disposed above the bottom plate 120 via the rack connecting rod 130 .

[0053] like Figure 1 As shown, with such an arrangement, an escape space is formed between the storage plate 110 and the bottom plate 120. When changing the relative position of the claw deployment fixing mechanism 200 and the storage plate 110 and the height of the clamping space, the escape space facilitates the operator's operation, thereby avoiding twisting the connecting nut 233 located under the storage plate 110 and providing an escape space for the connecting rod 231, thereby improving the convenience of operation.

[0054] In some other embodiments, in order to ensure the anti-vibration effect of the anti-vibration platform 400, the bottom plate 120 and the frame connecting rod 130 may not be provided, and the storage plate 110 may be directly fixedly connected to the upper end surface of the anti-vibration platform 400. Figure 3 shown.

[0055] Furthermore, the storage plate 110 is movably connected to the end of the frame connecting rod 130 away from the bottom plate 120 through the auxiliary hole 111, and the auxiliary hole 111 is set as a long hole extending along the X direction. In this way, when the storage plate 110 is installed above the bottom plate 120 and the claw deployment fixing mechanism 200 is connected to the storage plate 110, the relative position of the auxiliary hole 111 and the frame connecting rod 130 can be changed in the X direction according to the relative position of the imaging member 300 and the claw deployment fixing mechanism 200, so as to complete the position fine adjustment of the storage plate 110 and the claw deployment fixing mechanism 200 in the X direction, so that the imaging center of the imaging member 300 is directly opposite to the observation glass 212, thereby further improving the accuracy of the results of observation and evaluation of the claw of the target animal.

[0056] Preferably, the frame connecting rod 130 is set as a threaded connecting rod, and correspondingly, two connecting rod nuts 140 are set on the threaded connecting rod. After the threaded connecting rod is connected to the storage plate 110, the two connecting rod nuts 140 are respectively located on both sides of the storage plate 110. When it is necessary to change the height of the observation slide 212 according to the height of the imaging member 300 to improve the observation effect of the claw of the target animal, the two connecting rod nuts 140 are screwed at the same time to change the relative position of the threaded connecting rod and the storage plate 110. Alternatively, the frame connecting rod 130 is directly set as a telescopic connecting rod, and the relative position of the bottom plate 120 and the storage plate 110 is changed by changing the telescopic amount of the telescopic connecting rod itself.

[0057] The two-photon microscope imaging fixation device provided in this embodiment can not only perform fixed observation on anesthetized mice of different sizes, but also perform fixed observation on rabbits and cats in anesthesia or awake state waiting for observation according to actual needs, and the types of animals to be fixedly observed are not limited to this. In order to further improve the use effect of the two-photon microscope imaging fixing device, in this embodiment, the length of the placement plate 110 is set to 200mm, the width is set to 150mm, and the thickness is set to 15mm; the diameter of the frame connecting rod 130 is set to 6mm, and the size of the auxiliary hole 111 is set corresponding to the frame connecting rod 130; the diameter of the connecting rod 231 is set to 3mm, the size of the connecting hole 232 is set corresponding to the connecting rod 231, and along the length direction of the placement plate 110, the distance between two adjacent connecting holes 232 is set to 25mm, and along the width direction of the placement plate 110, the distance between two adjacent connecting holes 232 is set to 100mm; the observation slide 212 is set to a circular plate with a diameter of 25mm, the support plate 211 is set corresponding to the observation slide 212, the diameter of the adjusting screw 221 is set to 3mm, and the diameter of the adjusting hole is set to 3.5mm; the size of each nut is set according to the size of the corresponding rod, which will not be repeated here. In some other embodiments, the relative sizes of the components in the two-photon microscope imaging fixture can be adaptively adjusted according to actual needs, which is not limited to this example.

[0058] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. For those skilled in the art, various obvious changes, readjustments and substitutions can be made without departing from the protection scope of the present invention. It is not necessary and impossible to list all the embodiments here. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention shall be included in the protection scope of the claims of the present invention.

Claims

1. A two-photon microscope imaging fixture, characterized in that: include: A rack (100) is provided with a placement plate (110) for placing the animal to be observed; A claw deployment fixing mechanism (200) comprises a claw deployment component (210) and an adjustment component (220), wherein the claw deployment component (210) comprises a support plate (211) and an observation glass slide (212), wherein the support plate (211) is arranged on the storage plate (110), and the observation glass slide (212) is movably arranged on the support plate (211) through the adjustment component (220), and cooperates with the support plate (211) to form a clamping space with adjustable size, wherein the support plate (211) and the observation glass slide (212) can clamp the claw of the animal to be observed and keep the claw in an deployed state; An imaging member (300) is arranged above the observation glass slide (212), and the imaging member (300) is used to observe and photograph the claw portion deployed in the clamping space.

2. The two-photon microscope imaging fixture according to claim 1, characterized in that: The adjustment assembly (220) comprises an adjustment screw (221), the adjustment screw (221) is fixedly connected to the upper end surface of the support plate (211) and extends along the Z direction, and the observation glass slide (212) is movably connected to the adjustment screw (221) along the Z direction through an adjustment hole.

3. The two-photon microscope imaging fixture according to claim 2, characterized in that: Two adjusting screws (221) are arranged near the edge of the support plate (211), the adjusting holes are arranged corresponding to the adjusting screws (221), and the two adjusting screws (221) are arranged symmetrically about the center of the support plate (211).

4. The two-photon microscope imaging fixture according to claim 1, characterized in that: The claw deployment fixing mechanism (200) further comprises a connecting assembly (230), wherein the connecting assembly (230) comprises a connecting rod (231), a plurality of connecting holes (232) are provided at intervals on the storage plate (110), the support plate (211) is connected to the connecting rod (231), and the connecting rod (231) is detachably connected to any of the connecting holes (232).

5. The two-photon microscope imaging fixture according to claim 4, characterized in that: The plurality of connection holes (232) are arranged in a rectangular array on the storage plate (110), and each of the connection holes (232) is arranged as a long hole extending along the Y direction.

6. The two-photon microscope imaging fixture according to claim 4, characterized in that: The connecting rod (231) is configured as a connecting screw, the connecting assembly (230) further comprises a connecting nut (233), the supporting plate (211) is movably connected to the connecting screw via a connecting hole (232), the connecting nut (233) is threadedly connected to the connecting screw, and can adjust the relative position of the supporting plate (211) along the Z direction; Alternatively, the connecting rod (231) is configured as a telescopic rod, and the support plate (211) is fixedly connected to the connecting rod (231).

7. The two-photon microscope imaging fixture according to claim 4, characterized in that: The frame (100) further comprises a bottom plate (120) and a frame connecting rod (130), and the storage plate (110) is arranged above the bottom plate (120) via the frame connecting rod (130).

8. The two-photon microscope imaging fixture according to claim 7, characterized in that: The storage plate (110) is provided with an auxiliary hole (111), and the storage plate (110) is movably connected to an end of the frame connecting rod (130) away from the bottom plate (120) through the auxiliary hole (111), and the auxiliary hole (111) is configured as a long strip hole extending along the X direction.

9. The two-photon microscope imaging fixture according to claim 8, characterized in that: The frame connecting rod (130) is configured as a threaded connecting rod or a telescopic connecting rod.

10. The two-photon microscope imaging fixture according to any one of claims 1 to 9, characterized in that: The two-photon microscope imaging fixing device also includes an anti-vibration platform (400), and the frame (100) is fixedly connected to the upper end surface of the anti-vibration platform (400).

Images