Composition and product with beauty and skin care effects, preparation method of the composition and application thereof

Abstract

The present invention relates to the field of food technology, and more particularly to a composition and product having beauty and skin-nourishing effects, a preparation method for the composition, and applications thereof. The composition comprises the following components, measured by weight: 5-12 parts of fish collagen peptide, 0.1-0.5 parts of type III collagen peptide, 0.1-0.5 parts of skate collagen peptide, 1-3 parts of collagen tripeptide, 0.1-0.5 parts of bird's nest peptide, 0.1-0.3 parts of casein peptide, 0.1-0.5 parts of fish maw oligopeptide, and 0.01-0.05 parts of red and black currant mixed powder. The composition has the advantages of improving skin moisture, elasticity, and wrinkle removal effects, and is highly safe.

Description

Technical Field

[0001] The present invention relates to the field of food technology, and in particular to a composition and product with beauty and skin care effects, a preparation method of the composition and applications thereof. Background Art

[0002] Dermal fibroblasts synthesize and secrete collagen and elastin, producing collagen fibers and elastic fibers. They also synthesize and secrete matrix components such as glycosaminoglycans and glycoproteins. Collagen fibers, the primary component of the dermis, are highly resilient and tensile-resistant, allowing the skin to withstand significant tension, but lack elasticity. Elastic fibers impart elasticity to the skin, allowing it to easily return to its original shape after being stretched. The matrix fills the spaces between the fibers and cells. Within the dermis, collagen fibers and elastic fibers are interwoven and embedded within the matrix, maintaining the skin's firmness and elasticity. The fibrous tissue and matrix within the dermis are closely associated with skin density, plumpness, laxity, wrinkles, and dryness.

[0003] Due to natural aging and photoaging, the number and activity of dermal fibroblasts decrease with age, and the synthesis of collagen, elastin and hyaluronic acid decreases. The degradation of collagen fibers and elastic fibers in the skin accelerates, and their structures become abnormal or degenerate, causing problems such as dryness, sagging, and wrinkles on the skin. In particular, the large amount of matrix metalloproteinases caused by photoaging specifically degrade various collagens and elastins, thereby aggravating problems such as dryness, sagging and wrinkles.

[0004] There are relatively few beauty and health foods on the market today. They have single nutrition and limited functions. Their moisturizing and beauty effects are not ideal. In addition, they generally contain chemical agents that may harm the human body.

[0005] Chinese Patent Publication No. CN106901364A discloses a beauty and skincare health food and its preparation method. This health food is primarily composed of the following ingredients (by weight percentage): 45-50% soy protein oligopeptide, 2-5% angelica extract, 2-5% grape seed extract, and 2-5% oral hyaluronic acid. The combination of soy protein oligopeptide with angelica extract and grape seed extract enhances the health food's freckle-lightening and freckle-removing effects; the combination of soy protein oligopeptide with grape seed extract and hyaluronic acid enhances the health food's anti-aging, wrinkle-preventing, and wrinkle-reducing effects; and the combination of soy protein oligopeptide with hyaluronic acid enhances the health food's ability to improve dull skin and restore radiance.

[0006] Chinese patent publication number CN104489700A discloses a composition with the functions of improving skin moisture, elasticity and wrinkle removal. The active ingredients are mainly collagen peptides and elastin peptides as well as traditional Chinese medicine extracts. The traditional Chinese medicine extracts are prepared from astragalus, polygonatum, rehmannia root and rose as raw materials.

[0007] Although these products have improved skin moisture, elasticity, and wrinkle reduction to a certain extent, their effectiveness still needs to be further improved. Furthermore, these products use medicinal ingredients such as angelica sinensis, astragalus root, and raw rehmannia root, which may have certain side effects on the human body.

[0008] Therefore, it is very necessary to develop a composition and product with beauty and skin care effects that can solve the above technical problems, a preparation method of the composition and its application. Summary of the Invention

[0009] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a composition and product having a beauty and skin care effect, which has good effects on improving skin moisture, elasticity and wrinkle removal and is highly safe, as well as a preparation method and application of the composition.

[0010] The present invention is achieved through the following technical solutions:

[0011] A first aspect of the present invention provides a composition with beauty and skin care effects, characterized in that it comprises the following components, calculated by weight: 5-12 parts of fish collagen peptide, 0.1-0.5 parts of type III collagen peptide, 0.1-0.5 parts of skate collagen peptide, 1-3 parts of collagen tripeptide, 0.1-0.5 parts of bird's nest peptide, 0.1-0.3 parts of casein peptide, 0.1-0.5 parts of fish maw oligopeptide, and 0.01-0.05 parts of red and black currant mixed powder;

[0012] The preparation method of the fish maw oligopeptide comprises the following steps:

[0013] (1) Crush the fish maw, add water, and homogenize to obtain a homogenate;

[0014] (2) adding enzyme to the homogenate for enzymatic hydrolysis, and then inactivating the enzyme to obtain enzymatic hydrolyzate;

[0015] (3) After centrifuging the enzymatic hydrolysate, the hydrolysate is separated by membrane separation and dried;

[0016] The fish bladders in step (1) are sourced from croaker, yellow croaker and silver carp, and the mass ratio of the fish bladders of the three fish is 2-4:1-3:1;

[0017] In step (2), alkaline protease, papain and bromelain are added in sequence for enzymatic hydrolysis.

[0018] In some embodiments of the present invention, the composition is an oral composition.

[0019] In some embodiments of the present invention, the mass ratio of the collagen tripeptide, the bird's nest peptide and the fish maw oligopeptide is 10:1-2:1-2.

[0020] In some embodiments of the present invention, the mass ratio of the fish maw to water in step (1) is 1:5-10.

[0021] In some embodiments of the present invention, the pH of the homogenate is adjusted to 6.0-8.0 before enzymatic hydrolysis in step (2).

[0022] In some embodiments of the present invention, the amount of the enzyme used in step (2) is 2-4% of the mass of the homogenate.

[0023] In some embodiments of the present invention, the enzymes in step (2) are alkaline protease, papain and bromelain, and the mass ratio of the three is 2-4:1-3:4-6.

[0024] In some embodiments of the present invention, the temperature of the enzymatic hydrolysis in step (2) is 45-55° C., and the enzymatic hydrolysis time is 1.5-3 h.

[0025] In some preferred embodiments of the present invention, the specific process of enzymatic hydrolysis is as follows: first, add alkaline protease for enzymatic hydrolysis for 0.5-1 h, then add papain for enzymatic hydrolysis for 0.5-1 h, and finally add bromelain for enzymatic hydrolysis for 0.5-1 h.

[0026] In some embodiments of the present invention, the specific method of inactivating the enzyme in step (2) is not limited, for example, the enzyme is incubated in a boiling water bath for 10-20 minutes after enzymatic hydrolysis.

[0027] In some embodiments of the present invention, in step (3), the precipitate is removed by centrifugation to obtain a supernatant for membrane separation.

[0028] In some embodiments of the present invention, the molecular weight cut-off of the membrane in step (3) is 3000-5000 Da.

[0029] In some embodiments of the present invention, the drying method in step (3) is spray drying.

[0030] The second aspect of the present invention provides a method for preparing the above-mentioned composition, comprising the following steps: uniformly mixing the components to obtain the composition.

[0031] The third aspect of the present invention provides a product comprising the above composition and auxiliary materials.

[0032] In some embodiments of the present invention, the auxiliary materials include but are not limited to at least one of fruit and vegetable juice, amla powder, yeast extract, niacinamide, acidulant, thickener, preservative, food flavoring and sweetener.

[0033] In some embodiments of the present invention, the fruit and vegetable juice includes but is not limited to at least one of blood orange concentrated juice, concentrated apple juice, grapefruit juice, red grapefruit concentrated juice, and purple carrot juice.

[0034] In some embodiments of the present invention, the thickener includes but is not limited to xanthan gum.

[0035] In some embodiments of the present invention, the preservative includes but is not limited to potassium sorbate.

[0036] In some embodiments of the present invention, the sweetener includes but is not limited to at least one of erythritol and sucralose.

[0037] In some embodiments of the present invention, the acidulant includes but is not limited to at least one of DL-malic acid, citric acid and sodium citrate.

[0038] In some embodiments of the present invention, the product is a food or a health product.

[0039] In some embodiments of the present invention, the product comprises, by weight, 30-50 parts of the composition having beauty and skin-nourishing effects, 1-5 parts of blood orange concentrated juice, 5-10 parts of concentrated apple juice, 1-5 parts of grapefruit juice, 0.5-2 parts of red grapefruit concentrated juice, 0.5-2 parts of purple carrot juice, 0.1-1 part of emblica powder, 0.1-1 part of yeast extract, 0.01-0.015 parts of niacinamide, 0.5-2 parts of DL-malic acid, 0.5-2 parts of citric acid, 0.2-2 parts of sodium citrate, 0.2-1 parts of xanthan gum, 0.01-0.1 parts of potassium sorbate, 0.05-0.5 parts of food flavoring, 1-10 parts of erythritol, and 0.05-0.2 parts of sucralose.

[0040] The preparation process of the product comprises the following steps:

[0041] S1: dissolving the sweetener, thickener and the composition in 4-6 times the amount (based on the amount of the composition) of water at 80-90°C, stirring until completely dissolved to obtain a mixture A;

[0042] S2: adding the emblica powder, yeast extract, and niacinamide to the mixture A; after complete dissolution, slowly adding the fruit and vegetable juice, preservative, and acidulant; and mixing evenly to obtain the mixture B;

[0043] S3 is prepared by adding food flavoring to mixture B and sterilizing the mixture.

[0044] The fourth aspect of the present invention provides the use of the above composition in preparing a product with beauty and skin care effects.

[0045] In some embodiments of the present invention, the beauty and skin care includes but is not limited to at least one of improving skin moisture status and improving wrinkles.

[0046] In some embodiments of the present invention, the product is a food or a health product.

[0047] The beneficial effects of the present invention are:

[0048] The invention adopts a specific type of fish maw to prepare fish maw oligopeptides. The prepared fish maw oligopeptides are applied in beauty and skin care products, and have significant effects on improving skin moisture and wrinkles.

[0049] In addition, when fish maw oligopeptides are enzymatically hydrolyzed using specific enzymes in a specific order, their effects on improving skin moisture and wrinkles are further enhanced.

[0050] The present invention further optimizes the composition of the peptide-containing composition, especially the compounding of collagen tripeptide, bird's nest peptide and fish maw oligopeptide in a specific ratio, which significantly improves the skin's moisture condition and wrinkle improvement effects. DETAILED DESCRIPTION

[0051] The present invention will be further described below with reference to specific embodiments, and the advantages and features of the present invention will become clearer as the description proceeds. However, these embodiments are merely exemplary and do not constitute any limitation to the scope of the present invention. It should be understood by those skilled in the art that the details and forms of the technical solutions of the present invention may be modified or replaced without departing from the spirit and scope of the present invention, and such modifications and replacements fall within the scope of protection of the present invention.

[0052] The auxiliary materials used in the present invention are all conventional commercially available raw materials. The following auxiliary materials are provided as examples:

[0053] Blood orange juice concentrate was purchased from Wuhu Lequan Biotechnology Co., Ltd., concentrated apple juice was purchased from Sanmenxia Yuanfenduo Juice Co., Ltd., grapefruit juice was purchased from Shanghai Lecheng Biotechnology Co., Ltd., red grapefruit juice concentrate was purchased from Tianjin Zhenruguo Food Industry Co., Ltd., purple carrot juice was purchased from Henan Zhongda Hengyuan Biotechnology Co., Ltd., emblica powder was purchased from Shanghai Nuoshen Food Trading Co., Ltd., yeast extract was purchased from Shanghai Guanshuo Biotechnology Co., Ltd., niacinamide was purchased from Shanghai Licheng Nutrition Technology Co., Ltd., xanthan gum was purchased from Hangzhou Weichun Food Accessories Co., Ltd., and food flavors were purchased from Hasegawa Flavors (Shanghai) Co., Ltd.

[0054] The sources of raw materials used in the examples and comparative examples of the present invention are shown in Table 1.

[0055] Table 1 Raw material source information

[0056]

[0057] Example 1

[0058] A composition having a beautifying and nourishing effect, comprising the following components, calculated by weight: 5 parts fish collagen peptide, 0.1 part type III collagen peptide, 0.1 part skate collagen peptide, 1 part collagen tripeptide, 0.1 part bird's nest peptide, 0.1 part casein peptide, 0.1 part fish maw oligopeptide, and 0.01 part red and black currant mixed powder. The ingredients are uniformly mixed to obtain the composition.

[0059] The preparation method of the fish maw oligopeptide comprises the following steps:

[0060] (1) crushing the fish maw and adding 5 times the volume (m / m) of water, and homogenizing to obtain a homogenate; the fish maws are sourced from croaker, yellow croaker, and silver carp, and the mass ratio of the maws of the three fish is 2:1:1;

[0061] (2) The pH of the homogenate was adjusted to 6.0, and alkaline protease was first added for enzymatic hydrolysis for 0.5 h, papain was then added for enzymatic hydrolysis for 0.5 h, and finally bromelain was added for enzymatic hydrolysis for 0.5 h. The enzymatic hydrolysis temperature was 45 °C, and the mass ratio of alkaline protease, papain and bromelain was 2:1:4. The total amount of enzyme was 2% of the mass of the homogenate. After inactivation of the enzymes, the enzymatic hydrolyzate was obtained.

[0062] (3) The enzymatic hydrolysate was centrifuged to remove the precipitate, and the supernatant was separated by membrane separation using a membrane with a molecular weight cutoff of 3000 Da, and then spray-dried.

[0063] Example 2

[0064] A composition having a beautifying and nourishing effect, comprising the following components, calculated by weight: 12 parts fish collagen peptide, 0.5 parts type III collagen peptide, 0.5 parts skate collagen peptide, 3 parts collagen tripeptide, 0.5 parts bird's nest peptide, 0.3 parts casein peptide, 0.5 parts fish maw oligopeptide, and 0.05 parts red and black currant mixed powder. The ingredients are uniformly mixed to obtain the composition.

[0065] The preparation method of the fish maw oligopeptide comprises the following steps:

[0066] (1) crushing the fish maw and adding 10 times the volume (m / m) of water, and homogenizing to obtain a homogenate; the fish maws are sourced from croaker, yellow croaker, and silver carp, and the mass ratio of the maws of the three fish is 4:3:1;

[0067] (2) The pH of the homogenate was adjusted to 8.0, and alkaline protease was first added for enzymatic hydrolysis for 1 h, then papain was added for enzymatic hydrolysis for 1 h, and finally bromelain was added for enzymatic hydrolysis for 1 h. The enzymatic hydrolysis temperature was 55 °C, and the mass ratio of alkaline protease, papain and bromelain was 4:3:6. The total amount of enzyme was 4% of the mass of the homogenate. After inactivation of the enzymes, the enzymatic hydrolyzate was obtained.

[0068] (3) The enzymatic hydrolysate was centrifuged to remove the precipitate, and the supernatant was separated by membrane separation using a membrane with a molecular weight cutoff of 5000 Da, and then spray-dried.

[0069] Example 3

[0070] A composition having a cosmetic and skin-nourishing effect, comprising the following components, calculated by weight: 9 parts fish collagen peptide, 0.3 parts type III collagen peptide, 0.3 parts skate collagen peptide, 2 parts collagen tripeptide, 0.3 parts bird's nest peptide, 0.2 parts casein peptide, 0.3 parts fish maw oligopeptide, and 0.03 parts red and black currant mixed powder. The ingredients are uniformly mixed to obtain the composition.

[0071] The preparation method of the fish maw oligopeptide comprises the following steps:

[0072] (1) crushing the fish maw and adding 8 times the volume (m / m) of water, and homogenizing to obtain a homogenate; the fish maws are sourced from croaker, yellow croaker, and silver carp, and the mass ratio of the maws of the three fish is 3:2:1;

[0073] (2) The pH of the homogenate was adjusted to 7.0, and alkaline protease was first added for enzymatic hydrolysis for 0.75 h, papain was then added for enzymatic hydrolysis for 0.75 h, and finally bromelain was added for enzymatic hydrolysis for 0.75 h. The enzymatic hydrolysis temperature was 50 °C, and the mass ratio of alkaline protease, papain and bromelain was 3:2:5. The total amount of enzyme was 3% of the mass of the homogenate, and the enzyme was inactivated to obtain the enzymatic hydrolyzate.

[0074] (3) The enzymatic hydrolysate was centrifuged to remove the precipitate, and the supernatant was separated by membrane separation using a membrane with a molecular weight cutoff of 4000 Da, and then spray-dried.

[0075] Application Example 1

[0076] A beauty and skin care product, comprising 50 parts of the composition of Example 1, 5 parts of blood orange concentrated juice, 10 parts of concentrated apple juice, 5 parts of grapefruit juice, 2 parts of red grapefruit concentrated juice, 2 parts of purple carrot juice, 1 part of emblica powder, 1 part of yeast extract, 0.015 parts of niacinamide, 2 parts of DL-malic acid, 2 parts of citric acid, 2 parts of sodium citrate, 1 part of xanthan gum, 0.1 parts of potassium sorbate, 0.5 parts of food flavoring, 10 parts of erythritol, and 0.2 parts of sucralose.

[0077] The preparation process of the product comprises the following steps:

[0078] S1: dissolving erythritol, sucralose, xanthan gum and the composition in 6 times the amount (based on the amount of the composition) of water at 80° C., and stirring until completely dissolved to obtain a mixture A;

[0079] S2: adding emblica powder, yeast extract, and niacinamide to mixture A; after complete dissolution, slowly adding blood orange juice concentrate, apple juice concentrate, grapefruit juice, red grapefruit juice concentrate, purple carrot juice, potassium sorbate, DL-malic acid, citric acid, and sodium citrate; and mixing uniformly to obtain mixture B;

[0080] S3 is prepared by adding food flavoring to mixture B and sterilizing the mixture.

[0081] Application Example 2

[0082] A beauty and skin care product, comprising 30 parts of the composition of Example 2, 1 part of blood orange concentrated juice, 5 parts of concentrated apple juice, 1 part of grapefruit juice, 0.5 parts of red grapefruit concentrated juice, 0.5 parts of purple carrot juice, 0.1 part of emblica powder, 0.1 part of yeast extract, 0.01 part of niacinamide, 0.5 part of DL-malic acid, 0.5 part of citric acid, 0.2 part of sodium citrate, 0.2 part of xanthan gum, 0.01 part of potassium sorbate, 0.05 part of food flavoring, 1 part of erythritol, and 0.05 part of sucralose.

[0083] The preparation process of the product comprises the following steps:

[0084] S1: dissolving erythritol, sucralose, xanthan gum and the composition in 4 times the amount (based on the amount of the composition) of water at 90° C., and stirring until completely dissolved to obtain a mixture A;

[0085] S2: adding emblica powder, yeast extract, and niacinamide to mixture A; after complete dissolution, slowly adding blood orange juice concentrate, apple juice concentrate, grapefruit juice, red grapefruit juice concentrate, purple carrot juice, potassium sorbate, DL-malic acid, citric acid, and sodium citrate; and mixing uniformly to obtain mixture B;

[0086] S3 is prepared by adding food flavoring to mixture B and sterilizing the mixture.

[0087] Application Example 3

[0088] A beauty and skin care product, comprising 40 parts of the composition of Example 3, 3 parts of blood orange concentrated juice, 7 parts of concentrated apple juice, 3 parts of grapefruit juice, 1 part of red grapefruit concentrated juice, 1 part of purple carrot juice, 0.5 part of emblica powder, 0.5 part of yeast extract, 0.01 part of niacinamide, 1 part of DL-malic acid, 1 part of citric acid, 1 part of sodium citrate, 0.5 part of xanthan gum, 0.05 part of potassium sorbate, 0.2 part of food flavoring, 5 parts of erythritol, and 0.1 part of sucralose.

[0089] The preparation process of the product comprises the following steps:

[0090] S1: dissolving erythritol, sucralose, xanthan gum and the composition in 5 times the amount (based on the amount of the composition) of water at 85° C., and stirring until completely dissolved to obtain a mixture A;

[0091] S2: adding the emblica powder, yeast extract, and niacinamide to mixture A, and slowly adding blood orange juice concentrate, apple juice concentrate, grapefruit juice, red grapefruit juice concentrate, purple carrot juice, potassium sorbate, DL-malic acid, citric acid, and sodium citrate after complete dissolution, and mixing uniformly to obtain mixture B;

[0092] S3 is prepared by adding food flavoring to mixture B and sterilizing the mixture.

[0093] Comparative Example 1

[0094] The only difference from Example 3 is that in the preparation process of fish maw oligopeptide, the total amount of fish maw remains unchanged, and the sources of fish maw are different, namely, only Sciaenidae and yellow croaker, with a mass ratio of 3:2; other conditions are the same.

[0095] Comparative Example 2

[0096] The only difference from Example 3 is that in the preparation process of fish maw oligopeptide, the total amount of fish maw remains unchanged, and the sources of fish maw are different, namely, only Sciaenopsis striata and silver carp, with a mass ratio of 3:1; other conditions are the same.

[0097] Comparative Example 3

[0098] The only difference from Example 3 is that in the preparation process of fish maw oligopeptide, the total amount of fish maw remains unchanged, and the sources of fish maw are different, namely yellow croaker and silver carp, with a mass ratio of 2:1; other conditions are the same.

[0099] Comparative Example 4

[0100] The only difference from Example 3 is that the enzymatic hydrolysis order is different during the preparation of fish maw oligopeptides, and alkaline protease, bromelain and papain are added in sequence for enzymatic hydrolysis, and the other conditions are the same.

[0101] Comparative Example 5

[0102] The only difference from Example 3 is that the enzymatic hydrolysis order is different during the preparation of fish maw oligopeptides, and bromelain, alkaline protease and papain are added in sequence for enzymatic hydrolysis, and the other conditions are the same.

[0103] Comparative Example 6

[0104] The only difference from Example 3 is that the total mass of collagen tripeptide, bird's nest peptide and fish maw oligopeptide remains unchanged, the mass ratio is different, which is 10:3:0.5, and the other conditions are the same.

[0105] The compositions prepared in Comparative Examples 1 to 6 were used to replace the composition of Example 3 in Application Example 3, respectively, with the dosage remaining unchanged, and these compositions were used as Comparative Application Examples 1 to 6.

[0106] Test Example 1

[0107] Human food testing

[0108] Experimental plan: 100 women aged 35-45 were randomly divided into 10 groups, with 10 participants in each group. The experimental samples were taken for 30 days. The subjects' skin was tested using a skin tester before and after use.

[0109] Experimental sample: Take 25 mL daily according to the formula of each application example or comparative application example. The blank control group does not take any sample and has the same diet and daily routine as the experimental group.

[0110] Testing Method: Use a clean cotton ball soaked in distilled water to clean the skin (1.5 cm horizontally from the outer corner of the eye). After drying for 15 minutes, use the Real Bubee RBX-916 skin analyzer to measure skin moisture content. Use the DJM more me skin analyzer to measure skin wrinkles. The test results for each application example and comparative application example are shown in Table 2.

[0111] Table 2 Test results of application examples / comparative application examples

[0112]

[0113] Note: Comparison between blank control group and application examples 1-3 before and after the test, ** P <0.01; compared with application example 3, # P <0.05,## P <0.01.

[0114] Comparing Application Examples 1-3 with Application Example 3, the fish maw oligopeptides of Application Example 3 were prepared using fish bladders from three sources: stonehead cod, yellow croaker and silver carp, while Comparative Application Examples 1-3 only used any two of them, with the total amount of fish bladders remaining unchanged. As a result, Application Example 3 was significantly superior to Comparative Application Examples 1-3 in improving skin moisture status and wrinkle improvement effects, indicating that fish maw oligopeptides must be prepared using fish bladders from three sources: stonehead cod, yellow croaker and silver carp in order to achieve excellent results in improving skin moisture status and wrinkle improvement effects.

[0115] Comparing Application Example 4-Comparative Application Example 5 with Application Example 3, the enzymatic hydrolysis order of alkaline protease, papain and bromelain was different during the preparation of fish maw oligopeptides. As a result, Application Example 3 was significantly better than Comparative Application Example 4-Comparative Application Example 5 in improving skin moisture and wrinkle improvement. This indicates that a specific enzymatic hydrolysis order must be used to prepare fish maw oligopeptides in order to achieve excellent results in improving skin moisture and wrinkle improvement.

[0116] Comparing Application Example 6 with Application Example 3, the dosage ratios of collagen tripeptide, bird's nest peptide and fish maw oligopeptide are different. As a result, Application Example 3 is significantly better than Comparative Application Example 6 in improving skin moisture condition and improving wrinkles. This shows that a specific dosage ratio of collagen tripeptide, bird's nest peptide and fish maw oligopeptide must be used to achieve excellent results in improving skin moisture condition and improving wrinkles.

[0117] Test Example 2

[0118] Zebrafish assay

[0119] 1. Sample preparation information

[0120] The beauty and skin care products prepared in each application example and comparative application example are directly absorbed as the original liquid.

[0121] 2. Experimental Animals

[0122] Zebrafish were reared in aquaculture water at 28°C (water quality: conductivity 450-550 μS / cm; pH 6.5-8.5; hardness 50-100 mg / L CaCO3). Broodstock were purchased from Hangzhou Huante Biotechnology Co., Ltd., and juveniles were obtained by natural breeding of broodstock.

[0123] 3. Instruments and consumables

[0124] Dissecting microscope (SZX7, OLYMPUS, Japan); CCD camera (VertA1, Shanghai Tusen Vision Technology Co., Ltd.); precision electronic balance (CP214, OHAUS, USA); 6-well plate (Zhejiang Bellanbo Biotechnology Co., Ltd.).

[0125] 4. Evaluation of hydration efficacy

[0126] 2-day-old zebrafish of the Albino strain with a melanin allele mutation were randomly selected and plated in a 6-well plate. Thirty zebrafish were treated in each well (experimental group). The experimental group received 12 μL / mL of the cosmetic skincare product of the present invention in water. A normal control group and a model control group were also established, with each well containing 3 mL. All other groups, except the normal control group, received sodium chloride in water to establish a zebrafish dehydration model. After treatment at 28°C for 22 hours, ten randomly selected zebrafish from each experimental group were photographed under a dissecting microscope. Data were collected and analyzed using NIS-Elements D 3.20 advanced image processing software. Tail area was analyzed, and the hydrating efficacy of the samples was evaluated using statistical analysis of this indicator. Statistical results are expressed as mean ± SE. Statistical analysis was performed using SPSS 26.0 software. The results are shown in Table 3.

[0127] Table 3 Zebrafish tail area test results

[0128]

[0129] Note: Compared with the model control group, * P <0.05,** P <0.01,*** P <0.001; compared with application example 3, # P <0.05.

[0130] Test Example 3

[0131] Stability testing

[0132] Place 20 mL of the product of Application Examples 1 to 3 in a centrifuge and centrifuge at 2000 rpm for 10 min. Quickly aspirate the supernatant and measure the absorbance at a wavelength of 750 nm using a UV-visible spectrophotometer. Test the absorbance before and after centrifugation separately. Measure each sample 3 times and take the average value.

[0133] The stability calculation formula is: Stability W=A 后 / A0*100%;

[0134] Among them, A 后 W represents the absorbance after centrifugation, and A0 represents the absorbance before centrifugation. A higher W value indicates better product stability. The results are shown in Table 4.

[0135] Table 4 Stability test results of application example products

[0136]

[0137] As can be seen from Table 4, the product of the present invention has good stability. In addition, the product of the present invention has uniform color, no suspended matter, and no stratification.

[0138] The above detailed description is a specific description of one feasible embodiment of the present invention. This embodiment is not intended to limit the patent scope of the present invention. Any equivalent implementation or modification that does not depart from the present invention should be included in the scope of the technical solution of the present invention.

Claims

1. A product with beauty and skin care effects, comprising auxiliary materials and a composition, characterized in that: The composition comprises the following components in parts by weight: 5-12 parts of fish collagen peptide, 0.1-0.5 parts of type III collagen peptide, 0.1-0.5 parts of skate collagen peptide, 1-3 parts of collagen tripeptide, 0.1-0.5 parts of bird's nest peptide, 0.1-0.3 parts of casein peptide, 0.1-0.5 parts of fish maw oligopeptide, and 0.01-0.05 parts of red and black currant mixed powder; the mass ratio of the collagen tripeptide, bird's nest peptide and fish maw oligopeptide is 10:1-2:1-2; The preparation method of the fish maw oligopeptide comprises the following steps: (1) Crush the fish maw, add water, and homogenize to obtain a homogenate; (2) adding enzyme to the homogenate for enzymatic hydrolysis, and then inactivating the enzyme to obtain enzymatic hydrolyzate; (3) After centrifuging the enzymatic hydrolysate, the enzymatic hydrolysate is separated by membrane separation and dried; The fish bladders in step (1) are sourced from croaker, yellow croaker and silver carp, and the mass ratio of the fish bladders of the three fish is 2-4:1-3:1; In step (2), alkaline protease, papain and bromelain are added in sequence for enzymatic hydrolysis.

2. The product according to claim 1, characterized in that The mass ratio of the fish maw to water in step (1) is 1:5-10.

3. The product according to claim 1, characterized in that In step (2), the pH of the homogenate is adjusted to 6.0-8.0 before enzymatic hydrolysis; the amount of the enzyme used is 2-4% of the mass of the homogenate; the temperature of the enzymatic hydrolysis is 45-55° C., and the enzymatic hydrolysis time is 1.5-3 h.

4. The product according to claim 1, characterized in that The mass ratio of alkaline protease, papain and bromelain in step (2) is 2-4:1-3:4-6; the specific process of the enzymatic hydrolysis is as follows: first add alkaline protease for enzymatic hydrolysis for 0.5-1h, then add papain for enzymatic hydrolysis for 0.5-1h, and finally add bromelain for enzymatic hydrolysis for 0.5-1h.

5. The product according to claim 1, characterized in that In step (3), the precipitate is removed by centrifugation to obtain a supernatant for membrane separation; the molecular weight cutoff of the membrane is 3000-5000Da; and the drying method is spray drying.

6. The product according to any one of claims 1 to 5, characterized in that: The preparation method of the composition comprises the following steps: the components are mixed evenly to obtain the composition.

7. The product according to claim 1, characterized in that The auxiliary materials include at least one of fruit and vegetable juice, amla powder, yeast extract, nicotinamide, acidulant, thickener, preservative, food flavor and sweetener.

8. The product according to claim 7, characterized in that The fruit and vegetable juice includes at least one of blood orange concentrated juice, concentrated apple juice, grapefruit juice, red grapefruit concentrated juice, and purple carrot juice; the thickener includes xanthan gum; the preservative includes potassium sorbate; the sweetener includes at least one of erythritol and sucralose; and the acidulant includes at least one of DL-malic acid, citric acid, and sodium citrate.

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