Detection mechanism, blood coagulation analyzer and sample analysis method thereof

Abstract

The invention discloses a detection mechanism, a blood coagulation analyzer and a sample analysis method.The detection mechanism comprises an incubation detection disc assembly which is of a rotating disc type structure and comprises a plurality of cup positions used for containing reaction cups, the cup position is internally provided with a sample placing position for receiving the reaction cup of which the sample is dispensed, at least one dispensing reagent position for dispensing a reagent into the reaction cup, at least one detection position for detecting and analyzing a mixture of the sample and the reagent in the reaction cup, and a cup throwing position for throwing the reaction cup of which the detection and analysis are completed; the reagent disc assembly is of a rotating disc type structure, is concentric with the incubation detection disc assembly and is used for bearing at least one required reagent container; the sample detection assembly is arranged around the incubation detection disc assembly, is arranged on the periphery of the detection position, and is used for detecting the incubated sample and reagent mixture in the reaction cup on the incubation detection disc assembly. The action overlapping areas are few, the operation risk is greatly reduced, and the operation smoothness of the mechanism is improved.

Description

Technical Field

[0001] The present invention relates to the field of biological detection technology, and in particular to a detection mechanism, a coagulation analyzer and a sample analysis method thereof. Background Art

[0002] In the existing technical solutions, the mechanisms for cup filling, liquid separation, transportation, incubation, detection, and cup throwing are arranged linearly, and there is a certain area overlap between the moving mechanisms; for different test items, the incubation time may be different, or the type or quantity of required reagents may be different, resulting in complicated movement scheduling of the above mechanisms, and unable to guarantee continuous and stable output (report results).

[0003] Especially for analyzers with higher test speeds, the scheduling and management of the analyzer is more difficult because there are more dispensing and rotating cup modules and more areas of overlapping module movements. When the motion scheduling is unreasonable in order to avoid motion risks, there are many empty cycles, which greatly affects the output of the analyzer report results. Summary of the invention

[0004] Based on this, it is necessary to propose a detection mechanism, a coagulation analyzer and a sample analysis method thereof to address the above problems.

[0005] A detection mechanism, comprising: an incubation detection disc assembly, a reagent disc assembly, and a sample detection assembly;

[0006] The incubation detection disk assembly is a turntable structure, including a plurality of cup positions for accommodating reaction cups, wherein the cup positions are provided with a sample placement position for receiving a reaction cup after sample dispensing, at least one reagent dispensing position for dispensing reagents into the reaction cup, at least one detection position for detecting and analyzing the sample and reagent mixture in the reaction cup, and a cup discarding position for discarding the reaction cup after detection and analysis;

[0007] The reagent disc assembly is a turntable structure, which is concentrically arranged with the incubation detection disc assembly and is used to carry at least one required reagent container;

[0008] The sample detection component is arranged around the incubation detection tray component and at the periphery of the detection position, and is used to detect the sample and reagent mixture that has completed incubation in the reaction cup on the incubation detection tray component.

[0009] Preferably, the number of cup positions of the incubation detection tray assembly includes the sum of the first interval cup positions and the third interval cup positions, or the sum of the first interval cup positions, the second interval cup positions and the third interval cup positions.

[0010] Preferably, the first interval cup position is at least the quotient of the incubation time divided by the rotation cycle of the detection mechanism, the second interval cup position is at least the quotient of the reagent time when triggered divided by the rotation cycle of the detection mechanism, and the third interval cup position is at least the quotient of the detection time divided by the rotation cycle of the detection mechanism.

[0011] Preferably, the sample detection assembly includes an optical detection assembly, the number and position of which match the detection position, and is used to perform several detections on the sample and reagent mixture that has completed incubation in the reaction cup on the incubation detection tray assembly by an optical method;

[0012] And / or, the sample detection component structure also includes a magnetic bead detection component, which is arranged on the periphery of the incubation detection tray component and is specifically used to detect the reaction cup transferred from the incubation detection tray component by the magnetic bead method.

[0013] A coagulation analyzer comprises: the above-mentioned detection mechanism.

[0014] Preferably, it also includes a sample separation mechanism, which is arranged at the periphery of the sample placement position of the detection mechanism, and is used to dispense the sample into the reaction cup; and / or, the liquid separation mechanism is arranged around the detection mechanism, and is respectively arranged at the periphery of at least one reagent dispensing position of the detection mechanism, and is used to absorb a specified amount of reagent after an interval and dispense it into the reaction cup.

[0015] Preferably, it also includes a reaction cup transport mechanism, which is arranged around the detection mechanism and is respectively arranged at the sample placement position of the detection mechanism and the periphery of at least one reagent dispensing position, and is used to transport the reaction cup that has completed sample dispensing to the sample placement position and grab the reaction cup at the reagent dispensing position for reagent injection.

[0016] A sample analysis method applied to the coagulation analyzer, the method comprising:

[0017] Controlling the reaction cup of the sample that has been dispensed to be placed into the detection mechanism to obtain the reaction cup containing the sample;

[0018] After the first interval time, controlling the dispensing of reagent into the reaction cup containing the sample to obtain a reaction cup containing a mixture of the sample and the reagent;

[0019] The detection mechanism is controlled to perform coagulation analysis on the reaction cup containing the sample and reagent mixture, and the coagulation analysis result is output.

[0020] Preferably, the method further comprises:

[0021] After the first interval time, controlling the first reagent to be dispensed into the reaction cup containing the sample to obtain a reaction cup containing a mixture of the sample and the first reagent;

[0022] After the second interval time, controlling the injection of the second reagent into the reaction cup containing the mixture of the sample and the first reagent to obtain a reaction cup containing the mixture of the sample, the first reagent and the second reagent;

[0023] Controlling the detection mechanism to perform coagulation analysis on the reaction cup containing the sample, the first reagent and the second reagent mixture, and outputting the coagulation analysis result;

[0024] The outputting of the coagulation analysis result comprises: analyzing the optical coagulation analysis result, and if the optical coagulation analysis result meets the preset requirements, using the optical coagulation analysis result as the coagulation analysis result;

[0025] If the optical coagulation analysis result meets the preset requirement, the retest mode is started according to the retest rule, and after the retest result meets the preset requirement, the retest result is used as the coagulation analysis result.

[0026] Preferably, the first interval time is the product of the number of cup positions between the sample placement position and the first reagent dispensing position on the detection mechanism multiplied by the rotation period of the detection mechanism; the second interval time is the product of the number of cup positions between the first reagent dispensing position and the second reagent dispensing position on the detection mechanism multiplied by the rotation period of the detection mechanism.

[0027] The embodiments of the present invention have the following beneficial effects:

[0028] The reagent is injected into the reaction cup in the incubation detection disc assembly through the reagent disc assembly, and the incubation detection disc assembly completes the incubation of the sample or the mixture of the sample and the reagent. Afterwards, the sample or the mixture of the sample and the reagent that has completed the incubation in the incubation detection disc assembly is detected through the sample detection assembly to obtain an analysis result; the incubation detection disc assembly realizes both an incubation and detection platform, and is coaxial with the reagent disc assembly. The speed can be increased directly by increasing the number of cup positions of the incubation detection disc assembly, thereby reducing the difficulty of research and development. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0030] in:

[0031] Figure 1 A schematic diagram of a structure of a detection mechanism in an embodiment;

[0032] Figure 2 is a schematic structural diagram of another form of a detection mechanism in one embodiment;

[0033] Figure 3 A schematic diagram of a structure of a detection mechanism for a single reagent in an embodiment;

[0034] Figure 4 A schematic diagram of the structure of a detection mechanism for a dual reagent in one embodiment;

[0035] Figure 5 A schematic diagram of another structure of a detection mechanism for dual reagents in one embodiment;

[0036] Figure 6 A flow chart of a sample analysis method for a single reagent in one embodiment;

[0037] Figure 7 The present invention is a flow chart of a sample analysis method for double reagents in one embodiment. DETAILED DESCRIPTION

[0038] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0039] The embodiment of the present invention provides a detection mechanism, such as Figure 1 As shown, it includes: an incubation detection disc assembly 1, a reagent disc assembly 2, and a sample detection assembly 3;

[0040] The incubation detection disk assembly 1 is a turntable structure, including a plurality of cup positions 101 for accommodating reaction cups, wherein the cup positions 101 are provided with a sample placement position 102 for receiving a reaction cup after sample dispensing, at least one reagent dispensing position 103 for dispensing reagents into the reaction cup, at least one detection position 104 for detecting and analyzing the reaction cup, and a cup throwing position 105 for discarding the reaction cup after detection and analysis;

[0041] The reagent disk assembly 2 is a turntable structure, which is concentrically arranged with the incubation detection disk assembly 1 and is used to carry at least one required reagent container;

[0042] The sample detection assembly 3 is disposed around the incubation detection tray assembly 1 and at the periphery of the detection position 104 , and is used to detect the reaction cup on the incubation detection tray assembly 1 .

[0043] The reagent is injected into the reaction cup in the incubation detection disc assembly 1 through the reagent disc assembly 2, and the incubation detection disc assembly 1 completes the incubation of the sample or the mixture of the sample and the reagent. After that, the sample or the mixture of the sample and the reagent that has completed the incubation in the incubation detection disc assembly 1 is detected through the sample detection assembly 3 to obtain the analysis result; the incubation detection disc assembly 1 realizes both the incubation and detection platform, and is coaxial with the reagent disc assembly 2. The speed can be increased directly by increasing the number of cup positions of the incubation detection disc assembly, thereby reducing the difficulty of research and development.

[0044] The incubation detection tray assembly 1 has a plurality of cup positions 101 for accommodating reaction cups evenly arranged along the circumferential direction. As the incubation detection tray assembly 1 rotates once in each cycle, after the rotation stops, a cup position 101 is advanced on the incubation detection tray assembly 1. However, the sample placement position 102, the reagent dispensing position 103, the detection position 104, and the cup throwing position 105 remain fixed and always correspond to the cup position 101 at the corresponding position.

[0045] The reagent disc assembly 2 includes an inner disc 201 and / or an outer disc 202, wherein the inner disc 201 is arranged on the inner circle of the outer disc 202, and the outer disc 202 is arranged on the inner circle of the incubation detection disc assembly 1. Alternatively, the inner disc 201 and the outer disc 202 are arranged on the inner circle and outer circle of the incubation detection disc assembly 1, respectively.

[0046] The inner disk 201 and / or the outer disk 202 are coaxially arranged with the incubation detection disk assembly 1 and rotate synchronously or independently.

[0047] Specifically, for a single reagent, the inner disk 201 can be used as a reagent storage disk. During use, according to the research and development design requirements, the inner disk 201 can rotate or remain stationary;

[0048] In one embodiment, the inner disk 201 is stationary, which makes it easier to design the position of the reagent dispensing device and simplifies the control logic of the reagent dispensing device.

[0049] When a single reagent is used, the outer disc 202 can be used as a reagent containing disc. During use, according to the research and development design requirements, the outer disc 202 can rotate or remain stationary.

[0050] In one embodiment, the outer disk 202 is stationary, which makes it easier to design the position of the reagent dispensing device and simplify the control logic of the reagent dispensing device. In addition, the outer disk 202 is closer to the reagent dispensing device arranged on the periphery than the inner disk 201, shortening the action path of the reagent dispensing device to absorb and dispense the reagent.

[0051] When using two reagents, the inner disk 201 can be used as a reagent containing disk for the first reagent, and the outer disk 202 can be used as a reagent containing disk for the second reagent, or the outer disk 202 can be used as a reagent containing disk for the first reagent, and the inner disk 201 can be used as a reagent containing disk for the second reagent; during use, according to the needs of research and development and design, the inner disk 201 and the outer disk 202 rotate synchronously, or the inner disk 201 and the outer disk 202 rotate independently; by changing different rotation modes, it is easy to match the corresponding scheduling management of the reagent dispensing device and reduce the scheduling difficulty.

[0052] In one embodiment, the ratio between the rotation speed of the inner disk 201 and the rotation speed of the outer disk 202 is equal to the ratio between the diameter of the inner disk 201 and the diameter of the outer disk 202, so that the inner disk 201 and the outer disk 202 can rotate synchronously, realizing the linkage setting of the inner disk 201 and the outer disk 202 to meet the scheduling requirements of the reagent dispensing device.

[0053] In another embodiment, the outer disk 202 rotates counterclockwise at one speed, and the inner disk 201 rotates clockwise at another speed. Alternatively, the inner disk 201 and the outer disk 202 rotate in the same direction to achieve independent rotation of the inner disk 201 and the outer disk 202 to meet the scheduling requirements of the reagent dispensing device.

[0054] When a single reagent is used, one reagent dispensing position 103 is provided, and the number of cup positions of the incubation detection disc assembly 1 includes the sum of the first interval cup position and the third interval cup position.

[0055] On the one hand, by separately changing the first interval cup position and / or the third interval cup position to improve the detection speed, it is easier to achieve high-speed output, reducing the difficulty of developing a high-speed analyzer; on the other hand, by solidifying the time of reagent injection and cup throwing process through the sum of the first interval cup position and the third interval cup position, the difficulty of subsequent scheduling is reduced, making it easier to obtain continuous and stable output.

[0056] The first interval cup position is the cup position 101 spaced between the sample placement position 102 and the reagent dispensing position 103 in the counterclockwise direction, and the third interval cup position is the cup position 101 spaced between the reagent dispensing position 103 and the cup throwing position 105 in the counterclockwise direction.

[0057] The first interval cup position is at least the quotient of the incubation time divided by the rotation period of the detection mechanism, which determines the minimum value of the first interval cup, thereby increasing the number of cup positions 101 based on the minimum value, that is, expanding the cup positions 101 to meet more detection quantities and achieve speed improvement.

[0058] For example, there are m cup positions 101 spaced between the sample placement position 102 and the reagent dispensing position 103 in the counterclockwise direction, and the incubation detection disk assembly 1 needs to rotate m cycles, each rotation cycle is t seconds, and the incubation time after the sample is dispensed from the sample placement position 102 is t1, so the first interval cup positions are at least m=t1 / t;

[0059] If t1=360 seconds and t=15 seconds, then the number of first interval cup positions is at least m=360 / 15=24.

[0060] The third interval cup position is at least the quotient of the detection time divided by the rotation period of the detection mechanism, which determines the minimum value of the third interval cup, thereby increasing the number of cup positions 101 based on the minimum value, that is, expanding the cup positions 101 to meet more detection quantities and achieve speed improvement.

[0061] Exemplarily, the n cup positions 101 spaced between the reagent dispensing position 103 and the cup throwing position 105 in the counterclockwise direction require the incubation detection disk assembly 1 to rotate n cycles, each rotation cycle is t seconds, and after the reagent is dispensed from the reagent dispensing position 103, the detection time of the reagent dispensing position 103 is t2, so the third interval cup position is at least n=t2 / t;

[0062] If t2=210 seconds and t=15 seconds, then the number of third interval cup positions is at least n=210 / 15=14.

[0063] The number of detection positions 104 is at least the quotient of the detection time divided by the rotation period of the detection mechanism, that is, the detection position 104 is actually located at the third interval cup position, which is at least the minimum value of the third interval cup position, that is, n cup positions 101, and the minimum value of the input of the detection position 104 is determined, so that the number of cup positions 101 is increased on the basis of the minimum value, that is, the cup positions 101 are expanded to meet more detection quantities and achieve speed improvement.

[0064] The sample detection component 3 includes an optical detection component 301 . A plurality of optical detection components 301 are provided, and are specifically used to perform a plurality of detections on the reaction cups that have completed incubation on the incubation detection tray component 1 by an optical method.

[0065] The number of the optical detection components 301 is the same as the number of the detection positions 104 .

[0066] Since the incubation detection disk assembly 1 rotates once per cycle and advances one cup position 101, each reaction cup carrying a sample uses multiple optical detection assemblies 301 during the detection process. In order to ensure that the optical signals in the complete reaction process are collected as much as possible, the rotation time of the incubation detection disk assembly 1 is required to be as short as possible, such as 0.2s. If the rotation cycle is 15 seconds, the reaction cup to be tested is measured in the first optical detection assembly 301 for about 15-0.2=14.8s. In the next cycle, the incubation detection disk assembly 1 rotates and advances one cup position 101. Excluding the turntable time, the reaction cup to be tested is measured in the second optical detection assembly 301 for 15-0.2=14.8s, and so on until the test is completed. The number of the optical detection assemblies 301 depends on the longest detection time. If the longest detection time is 210s, the optical detection assemblies 301 should not be less than 210 / 15=14.

[0067] For double reagents, two reagent dispensing positions 103 are provided, namely, a first reagent dispensing position 1031 and a second reagent dispensing position 1032 . The number of cup positions of the incubation detection tray assembly 1 includes the sum of the first interval cup position, the second interval cup position and the third interval cup position.

[0068] On the one hand, by individually changing the first spacing cup position, the second spacing cup position and / or the third spacing cup position to improve the detection speed, it is easier to achieve high-speed output, reducing the difficulty of developing a high-speed analyzer; on the other hand, by solidifying the time of the first reagent injection, the second reagent injection and the cup throwing process through the sum of the first spacing cup position, the second spacing cup position and the third spacing cup position, it is reduced The difficulty of subsequent scheduling is convenient for obtaining continuous and stable output.

[0069] The first interval cup position is the cup position 101 interval between the sample placement position 102 and the first dispensing reagent position 1031 in the counterclockwise direction, the second interval cup position is the cup position 101 interval between the first dispensing reagent position 1031 and the second dispensing reagent position 1032 in the counterclockwise direction, and the third interval cup position is the cup position 101 interval between the second dispensing reagent position 1032 and the cup throwing position 105 in the counterclockwise direction.

[0070] The first interval cup position is at least the quotient of the incubation time divided by the rotation period of the detection mechanism, which determines the minimum value of the first interval cup, thereby increasing the number of cup positions 101 based on the minimum value, that is, expanding the cup positions 101 to meet more detection quantities and achieve speed improvement.

[0071] For example, if there are m cup positions 101 spaced between the sample placement position 102 and the first reagent dispensing position 1031 in the counterclockwise direction, the incubation detection disk assembly 1 needs to rotate m cycles, each rotation cycle is t seconds, and the incubation time from the sample placement position 102 to the first reagent dispensing is t1, then the first interval cup positions are at least m=t1 / t;

[0072] If t1=90 seconds and t=15 seconds, then the number of first interval cup positions is at least m=90 / 15=6.

[0073] There are k cup positions 101 spaced between the first reagent dispensing position 1031 and the second reagent dispensing position 1032 in the counterclockwise direction, so the incubation detection disk assembly 1 needs to rotate k cycles, each rotation cycle is t seconds, and the incubation time from the first reagent dispensing position 1031 to the second reagent dispensing is t2, so the second interval cup positions are at least k=t2 / t;

[0074] If t2=180 seconds and t=15 seconds, then the number of second interval cup positions is at least k=180 / 15=12.

[0075] The third interval cup position is at least the quotient of the detection time divided by the rotation period of the detection mechanism, which determines the minimum value of the third interval cup, thereby increasing the number of cup positions 101 based on the minimum value, that is, expanding the cup positions 101 to meet more detection quantities and achieve speed improvement.

[0076] Exemplarily, in order to dispense the n cup positions 101 between the reagent dispensing position 103 and the cup throwing position 105 in the counterclockwise direction, the incubation detection disk assembly 1 needs to rotate n cycles, each rotation cycle is t seconds, and after the second reagent is dispensed from the second dispensing reagent position 1032, the detection time of the second dispensing reagent position 1032 is t3, then the third interval cup position is at least n=t3 / t;

[0077] If t3=210 seconds and t=15 seconds, then the number of third interval cup positions is at least n=210 / 15=14.

[0078] The number of the detection positions 104 is at least the quotient of the detection time divided by the rotation period of the detection mechanism. In fact, that is to say, the detection position 104 is actually located at the third interval cup position, which is at least the minimum value of the third interval cup position, that is, n cup positions 101.

[0079] The sample detection component 3 includes an optical detection component 301 . A plurality of optical detection components 301 are provided, and are specifically used to perform a plurality of detections on the reaction cups that have completed incubation on the incubation detection tray component 1 by an optical method.

[0080] The number of the optical detection components 301 is the same as the number of the detection positions 104 .

[0081] Since the incubation detection disk assembly 1 rotates once per cycle and advances one cup position 101, each reaction cup carrying a sample uses multiple optical detection assemblies 301 during the detection process. In order to ensure that the optical signals in the complete reaction process are collected as much as possible, the rotation time of the incubation detection disk assembly 1 is required to be as short as possible, such as 0.2s. If the rotation cycle is 15 seconds, the reaction cup to be tested is measured in the first optical detection assembly 301 for about 15-0.2=14.8s. In the next cycle, the incubation detection disk assembly 1 rotates and advances one cup position 101. Excluding the turntable time, the reaction cup to be tested is measured in the second optical detection assembly 301 for 15-0.2=14.8s, and so on until the test is completed. The number of the optical detection assemblies 301 depends on the longest detection time. If the longest detection time is 210s, the optical detection assemblies 301 should not be less than 210 / 15=14.

[0082] If the number of cup positions of the incubation detection disc assembly 1 is set according to the above embodiment, 3600 / 15=240 results of the seven coagulation tests can be continuously produced every hour.

[0083] Furthermore, whether it is for single reagent or double reagent, such as Figure 2 As shown, the sample detection component 3 also includes a magnetic bead detection component 302, which is arranged on the periphery of the incubation detection disk component 1 and is specifically used to detect the reaction cup transferred from the incubation detection disk component 1 by the magnetic bead method.

[0084] The magnetic bead detection component 302 can realize the combined detection of optical method and magnetic bead method. In this case, the reaction cup uses a magnetic bead cup, and the magnetic bead cup incubated by the incubation detection disk component 1 is transported to the magnetic bead detection component 302 for detection.

[0085] The embodiment of the present invention also provides a coagulation analyzer, such as Figure 3 , 4 As shown, including detection mechanism.

[0086] The detection mechanism is as described in the above embodiment and will not be elaborated here.

[0087] Furthermore, it also includes a sample dividing mechanism 4, which is arranged at the periphery of the sample placement position of the detection mechanism and is used to divide the sample into the reaction cup.

[0088] The sample sorting mechanism 4 is used to sequentially send the sample rack carrying the sample containers in the loading area to the barcode scanning position, the sample suction position, the unloading area, etc., and run to the sampling position to realize the scheduling of the sample containers;

[0089] Furthermore, it also includes a cup supply component 5 arranged at the periphery of the sample placement position of the detection mechanism;

[0090] The cup supply assembly 5 is used to sort the disordered reaction cups and then select the reaction cups, and send them to the scheduling plate to wait for the sample sorting mechanism 4 to sort the samples.

[0091] Furthermore, it also includes a liquid dispensing mechanism 6, which is arranged around the detection mechanism and is respectively arranged at the periphery of at least one reagent dispensing position 103 of the detection mechanism, for sucking a specified amount of reagent at intervals and dispensing it into the reaction cup.

[0092] The sample separation mechanism 4, the cup supply assembly 5, and the liquid separation mechanism 6 are all arranged around the detection mechanism to reduce the overlapping area of ​​the actions of each mechanism and avoid interference.

[0093] For single reagent, such as Figure 3 As shown, the liquid dispensing mechanism 6 includes a sample liquid dispensing component 601 and a second reagent liquid dispensing component 603. The sample liquid dispensing component 601 is arranged at the periphery of the sample placement position 102, and is used to absorb a specified amount of sample or first absorb a certain amount of diluent and then absorb a specified amount of sample and dispense it into the reaction cup of the scheduling disk of the cup supply component 5; the second reagent liquid dispensing component 603 is arranged at the periphery of the reagent dispensing position 103, and is used to absorb a specified amount of reagent from the reagent disk component 2 and dispense it into the reaction cup on the incubation detection disk component 1;

[0094] For double reagents, such as Figure 4 As shown, the liquid dispensing mechanism 6 includes a sample liquid dispensing component 601, a first reagent liquid dispensing component 602, and a second reagent liquid dispensing component 603. The sample liquid dispensing component 601 is arranged at the periphery of the sample placement position 102, and is used to absorb a specified amount of sample or first absorb a certain amount of diluent and then absorb a specified amount of sample and dispense it into the reaction cup of the scheduling disk of the cup supply component 5; the first reagent liquid dispensing component 602 is arranged at the periphery of the first dispensing reagent position 1031, and is used to absorb a specified amount of first reagent from the reagent disk component 2 and dispense it into the reaction cup on the incubation detection disk component 1; the second reagent liquid dispensing component 603 is arranged at the periphery of the second dispensing reagent position 1032, and is used to absorb a specified amount of second reagent from the reagent disk component 2 and dispense it into the reaction cup on the incubation detection disk component 1.

[0095] The interval time from sample addition to reagent addition of the liquid dispensing mechanism 6 is unified, which simplifies the scheduling difficulty and facilitates obtaining continuous and stable output.

[0096] For single reagent and double reagent, the interval time of adding samples is unified, which is the rotation cycle of the detection mechanism, that is, every time the detection mechanism rotates one cycle, a specified amount of sample is sucked and dispensed into the reaction cup of the scheduling tray of the cup supply assembly 5.

[0097] Exemplarily, each rotation cycle is t seconds, t=15, then a specified amount of sample is drawn every 15 seconds and dispensed into the reaction cups on the scheduling tray of the cup supply assembly 5 .

[0098] For single reagent, such as Figure 3 As shown, the interval time from the sample placement position 102 when the reaction cup is dispensed with the sample to the reagent dispensing position 103 when the reagent is injected is m*t seconds, wherein m is the first interval cup position and t is the rotation period of the detection mechanism; the interval time from the reagent dispensing position 103 when the second reagent is dispensed with the cup throwing position 105 is n*t seconds.

[0099] Exemplarily, m=6, n=14, t=15, the interval time from the time the sample is dispensed into the reaction cup at the sample placement position 102 to the time the reagent is injected into the reagent dispensing position 103 is 6*15, i.e., 90 seconds, that is, the reagent is injected at least once every 90 seconds; the interval time from the time the second reagent is dispensed at the reagent dispensing position 103 to the time the cup is discarded at the cup disposal position 105 is 14*15, i.e., 210 seconds, that is, the detection is completed and the reaction cup is discarded at least every 210 seconds.

[0100] For double reagents, such as Figure 4 As shown, the interval time from the time when the sample is dispensed into the reaction cup from the sample placement position 102 to the time when the first reagent is injected into the first reagent dispensing position 1031 is m*t seconds, wherein m is the first interval cup position and t is the rotation period of the detection mechanism; the interval time from the time when the first reagent is dispensed into the first reagent dispensing position 1031 to the time when the second reagent is injected into the second reagent dispensing position 1032 is k*t seconds; and the interval time from the time when the second reagent is dispensed into the second reagent dispensing position 1032 to the time when the cup throwing position 105 is n*t seconds.

[0101] Exemplarily, m=6, k=12, n=14, t=15, the interval time from the time the sample is dispensed into the reaction cup from the sample placement position 102 to the time the first reagent is injected into the first reagent dispensing position 1031 is 6*18, i.e., 90 seconds; the interval time from the time the first reagent is dispensed into the first reagent dispensing position 1031 to the time the second reagent is injected into the second reagent dispensing position 1032 is 12*15, i.e., 180 seconds; the interval time from the time the second reagent is dispensed into the second reagent dispensing position 1032 to the cup throwing position 105 is 14*15, i.e., 210 seconds.

[0102] Alternatively, the interval time from the time the second reagent is dispensed from the second reagent dispensing position 1032 to the time the cup discarding position 105 reaches the reaction endpoint is determined by real-time analysis of the reaction curve. That is to say, the time interval for each project from the addition of the second reagent, that is, the triggering reagent to start the reaction to the output of the test result is not fixed. The reaction endpoint is determined by real-time analysis of the reaction curve. Once the reaction endpoint is found, the test can be terminated, the reaction cup can be discarded, and the test result can be reported. This not only reduces the occupancy time of the cup position 101, which is conducive to obtaining a higher speed output with fewer cup positions 101, but also shortens the report output time and the patient's waiting time.

[0103] Furthermore, it also includes a reaction cup transport mechanism 7, which is arranged around the detection mechanism and is respectively arranged at the sample placement position 102 and the periphery of at least one dispensing reagent position 103 of the detection mechanism, and is used to transport the reaction cup after sample dispensing to the sample placement position 102 and grab the reaction cup at the dispensing reagent position 103 for injecting reagent.

[0104] For single reagent, such as Figure 3 As shown, the reaction cup transport mechanism 7 is used to dispatch the reaction cup inside the coagulation analyzer, and includes a first grabbing hand 701, a third grabbing hand 703, and a fourth grabbing hand 704;

[0105] The first grabber 701 grabs the reaction cup after sample dispensing from the sampling position of the scheduling tray of the cup supply assembly 5 to the sample placement position 102 of the incubation detection tray assembly 1;

[0106] The third gripper 703 grabs the reaction cup that has completed incubation from the reagent dispensing position 103 of the incubation detection tray assembly 1, shakes it evenly after the reagent is injected into the liquid dispensing mechanism 6, and puts it back to the reagent dispensing position 103 of the incubation detection tray assembly 1;

[0107] The fourth gripper 704 grabs the reaction cup after the test from the cup throwing position 105 of the incubation test tray assembly 1 and transfers it to the cup throwing port for discarding.

[0108] For double reagents, such as Figure 4 As shown, the reaction cup transport mechanism 7 is used to dispatch the reaction cup inside the coagulation analyzer, including a first grabbing hand 701, a second grabbing hand 702, a third grabbing hand 703, and a fourth grabbing hand 704;

[0109] The first grabber 701 grabs the reaction cup after sample dispensing from the sampling position of the scheduling tray of the cup supply assembly 5 to the sample placement position 102 of the incubation detection tray assembly 1;

[0110] The second gripper 702 grabs the reaction cup that has completed incubation from the first reagent dispensing position 1031 of the incubation detection tray assembly 1, shakes it evenly after the first reagent is injected into the liquid dispensing mechanism 6, and puts it back to the first reagent dispensing position 1031 of the incubation detection tray assembly 1;

[0111] The third gripper 703 grabs the reaction cup that has completed incubation from the second reagent dispensing position 1032 of the incubation detection tray assembly 1, shakes it evenly after the second reagent is injected into the liquid dispensing mechanism 6, and puts it back into the second reagent dispensing position 1032 of the incubation detection tray assembly 1;

[0112] The fourth gripper 704 grabs the reaction cup after the test from the cup throwing position 105 of the incubation test tray assembly 1 and transfers it to the cup throwing port for discarding.

[0113] Furthermore, if Figure 5 As shown, when the sample detection component 3 further includes a magnetic bead detection component 302, the second reagent dispensing component 603 injects the sample into the dispensing reagent position 103 located at the incubation detection disk component 1 or the second dispensing reagent position 1032 of the incubation detection disk component 1 and washes it in the washing tank;

[0114] The third gripper 703 also grabs the magnetic bead cup from the second dispensing reagent position 1032 of the incubation detection disk assembly 1 to the magnetic bead method detection assembly 302 for incubation. After the magnetic bead method detection assembly 302 completes the incubation, the third gripper 703 grabs the magnetic bead cup from the magnetic bead method detection assembly 302 and transfers it to the top of the second dispensing reagent position 1032. The second reagent dispensing assembly 603 absorbs the first reagent from the reagent disk assembly 2 and moves to the second dispensing reagent position 1032 for dispensing. After the dispensing is completed, the second reagent dispensing assembly 603 is cleaned in the cleaning tank, and the third gripper 703 transfers the magnetic bead cup to the detection hole of the magnetic bead method detection assembly 302 for incubation.

[0115] The third gripper 703 grabs the magnetic bead cup from the magnetic bead cup supply rack and transfers it to the second reagent dispensing position 1032. The second reagent dispensing component 603 dispenses the sample. After the dispensing is completed, R2 goes to the cleaning tank for cleaning. The third gripper 703 transfers the magnetic bead cup to the detection hole of the magnetic bead detection component 302 for incubation.

[0116] After the sample incubation is completed, the third gripper 703 grabs the magnetic bead cup from the magnetic bead detection component 302 and transports it to the top of the second reagent dispensing position 1032, the second reagent dispensing component 603 absorbs the second reagent from the reagent disk component 2, and moves to the second reagent dispensing position 1032 for dispensing, after the dispensing is completed, the second reagent dispensing component 603 goes to the cleaning tank for cleaning, and the third gripper 703 transports the magnetic bead cup to the detection hole of the magnetic bead detection component 302 for incubation;

[0117] After the incubation is completed, the third gripper 703 grabs the magnetic bead cup from the magnetic bead detection component 302 and transports it to the top of the second dispensing reagent position 1032, the second reagent dispensing component 603 absorbs the R2 reagent from the inner circle of the reagent disk, and moves to the second dispensing reagent position 1032 for dispensing. After the dispensing is completed, the second reagent dispensing component 603 goes to the cleaning pool for cleaning, and the third gripper 703 transports the magnetic bead cup to the detection hole of the magnetic bead detection component 302 to start the detection; after the detection is completed, the third gripper 703 grabs the magnetic bead cup and transfers it to the cup mouth for throwing.

[0118] Furthermore, the present invention facilitates speed improvement by increasing the number of modules. For example, if an output of 600 per hour is to be achieved, the cycle time is 3600 / 600=6 seconds, the incubation time after sample dispensing is 90s, and the interval between the sample placement position 102 and the first dispensing reagent position 1031 is adjusted to m=90 / 6=15. The incubation time after dispensing the R1 reagent is 180s, and the interval between the first dispensing reagent position 1031 and the second dispensing reagent position 1032 is adjusted to n=180 / 6=30. The detection time after dispensing the second reagent is 210s, and the interval between the second dispensing reagent position 1032 and the cup throwing position 105 is adjusted to p=210 / 6=35.

[0119] The two first reagent dispensing components 602 are arranged in an axially symmetrical manner along the central axis of the first dispensing reagent position 1031 and the second gripper 702, and the action radius of each of the first reagent dispensing components 602 is tangent to the central axis. In this way, the two first reagent dispensing components 602 work alternately to ensure sufficient cleaning; it can also achieve speed improvement and reduce the overlapping area of ​​the actions of the two first reagent dispensing components 602 to avoid interference.

[0120] The two second reagent dispensing components 603 are arranged in an axially symmetrical manner along the central axis of the second dispensing reagent position 1032 and the third gripper 703, and the action radius of each of the second reagent dispensing components 603 is tangent to the central axis. In this way, the two second reagent dispensing components 603 work alternately to ensure sufficient cleaning; it can also achieve speed improvement and reduce the overlapping area of ​​the actions of the two second reagent dispensing components 603 to avoid interference.

[0121] The embodiment of the present invention further provides a sample analysis method, which is used in the coagulation analyzer described in the above embodiment, for a single reagent, such as Figure 6 As shown, the method is implemented by the following steps:

[0122] S101: Controlling the reaction cup of the sample that has been dispensed to be placed into the detection mechanism to obtain a reaction cup containing the sample;

[0123] S102: After the first interval time, controlling the injection of reagent into the reaction cup containing the sample to obtain a reaction cup containing a mixture of the sample and the reagent;

[0124] Specifically, the first interval time is the product of the number of cup positions between the sample placement position and the first reagent dispensing position on the detection mechanism multiplied by the rotation period of the detection mechanism.

[0125] After the first interval time, controlling the first reagent to be dispensed into the reaction cup containing the sample to obtain a reaction cup containing a mixture of the sample and the first reagent;

[0126] S103: Control the detection mechanism to perform coagulation analysis on the reaction cup containing the sample and reagent mixture, and output the coagulation analysis result.

[0127] Specifically, the optical detection component is controlled to perform coagulation analysis on the reaction cup that has completed incubation on the detection mechanism to obtain an optical coagulation analysis result, and the detection time of the optical detection component is determined by analyzing the reaction endpoint in the real-time reaction curve; and / or, the magnetic bead detection component is controlled to perform coagulation analysis on the reaction cup transferred from the detection mechanism to obtain a non-optical coagulation analysis result.

[0128] The outputting of the coagulation analysis result comprises: analyzing the optical coagulation analysis result, and if the optical coagulation analysis result meets the preset requirements, using the optical coagulation analysis result as the coagulation analysis result;

[0129] If the optical coagulation analysis result meets the preset requirement, the retest mode is started according to the retest rule, and after the retest result meets the preset requirement, the retest result is used as the coagulation analysis result.

[0130] The present invention also provides a sample analysis method for the coagulation analyzer described in the above embodiment. For double reagents, such as Figure 7 As shown, the method is implemented by the following steps:

[0131] S201: Controlling the reaction cup of the sample that has been dispensed to be placed into the detection mechanism to obtain a reaction cup containing the sample;

[0132] S202: After the first interval time, controlling the injection of reagent into the reaction cup containing the sample to obtain a reaction cup containing a mixture of the sample and the reagent;

[0133] Specifically, the first interval time is the product of the number of cup positions between the sample placement position and the first reagent dispensing position on the detection mechanism multiplied by the rotation period of the detection mechanism.

[0134] S203: After the second interval time, controlling the injection of the second reagent into the reaction cup containing the mixture of the sample and the first reagent to obtain a reaction cup containing the mixture of the sample, the first reagent and the second reagent;

[0135] Specifically, the second interval time is the product of the number of cup positions between the second reagent dispensing position and the third reagent dispensing position on the detection mechanism multiplied by the rotation period of the detection mechanism.

[0136] S204: Control the detection mechanism to perform coagulation analysis on the reaction cup containing the sample and reagent mixture, and output the coagulation analysis result.

[0137] Specifically, the optical detection component 301 is controlled to perform coagulation analysis on the reaction cup that has completed incubation on the detection mechanism to obtain an optical coagulation analysis result, and the detection time of the optical detection component 301 is determined by analyzing the reaction endpoint in the real-time reaction curve; and / or, the magnetic bead detection component 302 is controlled to perform coagulation analysis on the magnetic bead cup transferred from the detection mechanism to obtain a non-optical coagulation analysis result.

[0138] The outputting of the coagulation analysis result comprises: analyzing the optical coagulation analysis result, and if the optical coagulation analysis result meets the preset requirements, using the optical coagulation analysis result as the coagulation analysis result;

[0139] If the optical coagulation analysis result meets the preset requirement, the retest mode is started according to the retest rule, and after the retest result meets the preset requirement, the retest result is used as the coagulation analysis result.

[0140] The above disclosure is only the preferred embodiment of the present invention, which certainly cannot be used to limit the scope of the present invention. Therefore, equivalent changes made according to the claims of the present invention are still within the scope of the present invention.

Claims

1. A detection mechanism, characterized in that: include: Incubation detection disc assembly, reagent disc assembly, sample detection assembly; The incubation detection disk assembly is a turntable structure, including a plurality of cup positions for accommodating reaction cups, wherein the cup positions are provided with a sample placement position for receiving a reaction cup after sample dispensing, at least one reagent dispensing position for dispensing reagents into the reaction cup, at least one detection position for detecting and analyzing the sample and reagent mixture in the reaction cup, and a cup discarding position for discarding the reaction cup after detection and analysis; The reagent disc assembly is a turntable structure, which is concentrically arranged with the incubation detection disc assembly and is used to carry at least one required reagent container; The sample detection component is arranged around the incubation detection tray component and at the periphery of the detection position, and is used to detect the sample and reagent mixture that has completed incubation in the reaction cup on the incubation detection tray component.

2. The detection mechanism according to claim 1, characterized in that: The number of cup positions of the incubation detection tray assembly includes the sum of the first interval cup positions and the third interval cup positions, or the sum of the first interval cup positions, the second interval cup positions and the third interval cup positions.

3. The detection mechanism according to claim 2, characterized in that: The first interval cup position is at least the quotient of the incubation time divided by the rotation cycle of the detection mechanism, the second interval cup position is at least the quotient of the reagent time when triggered divided by the rotation cycle of the detection mechanism, and the third interval cup position is at least the quotient of the detection time divided by the rotation cycle of the detection mechanism.

4. The detection mechanism according to any one of claims 1 to 3, characterized in that: The sample detection assembly includes an optical detection assembly, the number and position of which match the detection position, and is used to perform several detections on the sample and reagent mixture that has completed incubation in the reaction cup on the incubation detection tray assembly by optical method; And / or, the sample detection component structure also includes a magnetic bead detection component, which is arranged on the periphery of the incubation detection tray component and is specifically used to detect the reaction cup transferred from the incubation detection tray component by the magnetic bead method.

5. A coagulation analyzer, characterized in that: include: A detection mechanism as claimed in any one of claims 1 to 4.

6. The coagulation analyzer according to claim 5, characterized in that: It also includes a sample dispensing mechanism, which is arranged at the periphery of the sample placement position of the detection mechanism and is used to dispense the sample into the reaction cup; and / or the liquid dispensing mechanism is arranged around the detection mechanism and is respectively arranged at the periphery of at least one reagent dispensing position of the detection mechanism, and is used to absorb a specified amount of reagent after an interval and dispense it into the reaction cup.

7. The coagulation analyzer according to claim 5, characterized in that: It also includes a reaction cup transport mechanism, which is arranged around the detection mechanism and is respectively arranged at the sample placement position of the detection mechanism and the periphery of at least one reagent dispensing position, and is used to transport the reaction cup after sample dispensing to the sample placement position and grab the reaction cup at the reagent dispensing position for reagent injection.

8. A sample analysis method used in a coagulation analyzer as claimed in any one of claims 5 to 7, characterized in that: The method includes: Controlling the reaction cup of the sample that has been dispensed to be placed into the detection mechanism to obtain the reaction cup containing the sample; After the first interval time, controlling the dispensing of reagent into the reaction cup containing the sample to obtain a reaction cup containing a mixture of the sample and the reagent; The detection mechanism is controlled to perform coagulation analysis on the reaction cup containing the sample and reagent mixture, and the coagulation analysis result is output.

9. The sample analysis method according to claim 8, characterized in that: The method further includes: After the first interval time, controlling the first reagent to be dispensed into the reaction cup containing the sample to obtain a reaction cup containing a mixture of the sample and the first reagent; After the second interval time, controlling the injection of the second reagent into the reaction cup containing the mixture of the sample and the first reagent to obtain a reaction cup containing the mixture of the sample, the first reagent and the second reagent; Controlling the detection mechanism to perform coagulation analysis on the reaction cup containing the sample, the first reagent and the second reagent mixture, and outputting the coagulation analysis result; And / or, the output coagulation analysis result includes: Analyzing the optical coagulation analysis result, and if the optical coagulation analysis result meets the preset requirements, using the optical coagulation analysis result as the coagulation analysis result; If the optical coagulation analysis result meets the preset requirement, the retest mode is started according to the retest rule, and after the retest result meets the preset requirement, the retest result is used as the coagulation analysis result.

10. The sample analysis method according to claim 9, characterized in that: The first interval time is the product of the number of cup positions between the sample placement position and the first reagent dispensing position on the detection mechanism multiplied by the rotation period of the detection mechanism; the second interval time is the product of the number of cup positions between the first reagent dispensing position and the second reagent dispensing position on the detection mechanism multiplied by the rotation period of the detection mechanism.

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