Application of Ectoin in oil control

Abstract

The invention provides an oil control composition and application thereof. The application finds that Ectoin has a remarkable inhibition effect on grease generation caused by linoleic acid. Therefore, Ectoine can be used as an oil-control active ingredient.

Description

Technical Field

[0001] The present application belongs to the field of cosmetic technology, and specifically, relates to the application of ectoine in oil control. Background Art

[0002] Linoleic acid (LA) is an unsaturated fatty acid with a molecular formula of C 18 H 32 O2, scientifically known as cis-9,12-octadecadienoic acid, is an essential fatty acid for the human body. Linoleic acid is an important component of phospholipids. It combines with other substances in cells and plays an important role in the function of cell membranes. Linoleic acid is an element in the formation of epidermal lipid barrier. Its insufficient function will have an important impact on cell proliferation, metabolic regulation and keratinization of epidermal cells.

[0003] However, the body cannot synthesize linoleic acid and must obtain it from the outside world such as food. However, since linoleic acid is a natural ligand of peroxisome proliferator-activated receptors (PPAR), the intake of linoleic acid will strongly promote the accumulation of lipid droplets in the cytoplasm, thereby increasing oil secretion. The large amount of lipid secretion by the sebaceous glands is also a necessary prerequisite for the occurrence and development of acne, which can further cause abnormal keratinization, microbial proliferation, and induce inflammation, resulting in an inflammatory cascade reaction and aggravating acne symptoms.

[0004] Therefore, how to reduce the sebum secretion caused by linoleic acid while ensuring the intake of linoleic acid so that it can play a better role in skin care and health care, thereby preventing / alleviating the formation of acne, pimples, blackheads, inflammatory papules, pustules, enlarged pores, dull skin tone, nodules, abscesses, dandruff, etc., is still a problem that needs to be solved urgently. Summary of the invention

[0005] The present application unexpectedly discovered that ectoine, which is generally believed to have no effective oil control effect, can inhibit and / or reduce the sebum production caused by linoleic acid, thereby providing a new application of this ingredient in improving skin conditions caused by excessive sebum secretion.

[0006] Specifically, this application involves the following aspects:

[0007] 1. Use of ectoine in inhibiting and / or reducing sebum production.

[0008] 2. Use of ectoine as an oil-controlling active agent in the preparation of a composition for external use on the skin.

[0009] 3. The use of Ecdoin as an oil control active agent.

[0010] 4. The use according to items 1 to 3, wherein the ectoine is used to inhibit and / or reduce sebum production caused by linoleic acid.

[0011] 5. The use according to items 1 to 4, wherein said ectoine is used for preventing and / or alleviating excessive sebum secretion.

[0012] 6. The use according to item 5, wherein the ectoine is used to prevent and / or alleviate the formation of one or more of acne, pimples, acne, blackheads, inflammatory papules, pustules, enlarged pores, dull skin tone, nodules, abscesses, and dandruff.

[0013] 7. A method for suppressing and / or removing sebum, comprising applying ectoine to the skin.

[0014] The present application found that Ecdoin has a significant inhibitory effect on sebum production caused by linoleic acid. Therefore, Ecdoin can be used as an oil control active ingredient. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 To show the effect of ectoine on sebum production under linoleic acid stimulation;

[0016] Figure 2 Showing the effect of ectoine on sebum production stimulated by arachidonic acid. DETAILED DESCRIPTION

[0017] The present application is further described below in conjunction with examples. It should be understood that the examples are only used to further describe and illustrate the present application and are not used to limit the present application.

[0018] Unless otherwise defined, the technical and scientific terms in this specification have the same meaning as those generally understood by those skilled in the art. Although similar or identical methods and materials as described herein may be used in experiments or practical applications, materials and methods are described herein below. In the event of a conflict, the present specification including the definitions therein shall prevail. In addition, materials, methods and examples are provided for illustration only and are not restrictive. The present application is further described below in conjunction with specific embodiments, but is not intended to limit the scope of the present application.

[0019] In view of the problems existing in the prior art, the present application provides the use of ectoine in inhibiting and / or reducing sebum production.

[0020] The composition of sebum is relatively complex, mainly containing waxes, triglycerides, free fatty acids, and phospholipids and cholesterol produced by epithelial cell metabolism. Sebum is oily and moisturizes the skin to prevent dryness and cracking. The fatty acids in sebum can kill bacteria and resist mold and viruses, and can be called the natural barrier of the skin. Sebum protects and moisturizes the skin, but too much sebum will form blackheads, acne, pimples, blackheads, inflammatory papules, pustules, enlarged pores, dull skin, nodules, abscesses, dandruff, etc., or appearance defects such as oily skin and rough skin.

[0021] The sebum content can be determined by any method known in the art, such as Nile red staining method and Oil red staining method.

[0022] The present application also provides the use of ectoine as an oil-controlling active agent in the preparation of a skin external composition.

[0023] Wherein, the skin external composition can be a cosmetic, a medicine, etc., which is not further limited in this application. It can be understood by those skilled in the art that the skin external composition, in addition to containing ectoine, can further include a physiologically acceptable carrier, an oil control component, a skin care component, etc. The oil control component includes but is not limited to one or more of salicylic acid, capryloyl salicylic acid, zinc hyaluronate, zinc chloride, etc.

[0024] In some specific embodiments, the content of ectoine in the skin topical composition is 0.0001wt%-3wt%, for example, it can be 0.0001wt%, 0.0005wt%, 0.001wt%, 0.005wt%, 0.01wt%, 0.02wt%, 0.03wt%, 0.04wt%, 0.05wt%, 0.06wt%, 0.07wt%, 0.08wt%, 0.09wt%, 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0. .5wt%, 0.6wt%, 0.7wt%, 0.8wt%, 0.9wt%, 1wt%, 1.1wt%, 1.2wt%, 1.3wt%, 1.4wt%, 1.5wt%, 1.6wt%, 1.7wt%, 1.8wt%, 1.9wt%, 2wt%, 2.1wt%, 2.2wt%, 2.3wt%, 2.4wt%, 2.5wt%, 2.6wt%, 2.7wt%, 2.8wt%, 2.9wt%, 3wt%, and any range between these values.

[0025] In some specific embodiments, the content of ectoine in the skin external composition is 0.001 wt%-0.1 wt%.

[0026] In some specific embodiments, the skin topical composition includes ectoine, wherein ectoine serves as an oil-controlling active agent and the content of ectoine is 0.0001 wt %-3 wt %.

[0027] In some specific embodiments, the skin topical composition includes ectoine, wherein ectoine serves as an oil-controlling active agent, the ectoine is used to inhibit and / or reduce sebum production caused by linoleic acid, and the content of the ectoine is 0.0001 wt%-3 wt%.

[0028] The present application also provides the use of ectoine as an oil control active agent.

[0029] In some specific embodiments, the use is the use of ectoine for inhibiting and / or reducing sebum production caused by linoleic acid.

[0030] In some specific embodiments, the use is the use of ectoine in preventing and / or alleviating excessive sebum secretion.

[0031] Excessive sebum secretion refers to an increase relative to normal sebum secretion, for example, an increase of 0.1%, 1%, 2%, 5%, 6%, 7%, 8%, 9%, or 10% or more.

[0032] It is known to those skilled in the art that excessive skin secretion may cause skin problems. For example, excessive sebum secretion may cause one or more of acne, pimples, blackheads, inflammatory papules, pustules, enlarged pores, dull skin tone, nodules, abscesses, dandruff, or appearance defects such as oily skin and rough skin.

[0033] The present application also provides a method for inhibiting and / or removing sebum, which comprises applying ectoine to the skin.

[0034] Existing studies generally believe that Ecdoin has no effective oil control effect. For example, Ecdoin has no obvious inhibitory effect on sebum production caused by arachidonic acid. However, the present application unexpectedly found that Ecdoin can effectively inhibit and / or reduce sebum production caused by linoleic acid, providing a new effect of Ecdoin in improving skin conditions caused by excessive sebum secretion caused by linoleic acid. In some scenarios, Ecdoin can be used as an oil control active ingredient.

[0035] Example

[0036] The present application provides a general and / or specific description of the materials and test methods used in the experiments. In the following examples, unless otherwise specified, % means wt%, i.e., weight percentage. The reagents or instruments used without indicating the manufacturer are all conventional reagent products that can be obtained commercially.

[0037] Weigh Ectoin (purchased from Bloomage Biotechnology Co., Ltd.) and mix with SZ95 cell (human sebaceous gland cell) complete culture medium (containing 10% FBS, 1% penicillin-streptomycin) to dissolve, and use it as the experimental group sample in the following experimental examples.

[0038] This application uses Nile red staining to determine sebum content. Nile red is the most ideal fat fluorescent dye, which is soluble in a variety of organic solvents and emits fluorescence, and has a high affinity for triglycerides and cholesterol. When used, the fat distribution and content can be statistically analyzed based on the changes in fluorescence intensity.

[0039] Experimental Example 1: Effect of Ecdoin on lipid production under stimulation of linoleic acid (LA)

[0040] Group settings:

[0041] Blank group: no LA stimulation treatment, no sample treatment

[0042] Control group: LA stimulation treatment, no sample treatment

[0043] Experimental group: LA stimulation treatment, 0.05% ectoine treatment

[0044] Test method: Take SZ95 cells and culture them in a T75 culture flask with DMEM medium. When the cell density is about 80%, inoculate them in a 24-well plate with a coverslip and culture them at 37°C and 5% CO2. After 24 hours, aspirate the supernatant, treat each well according to the above group settings, set 3 replicate wells for each group, and take 5 photos of each well randomly. Incubate in a 37°C, 5% CO2 incubator for 72 hours. Aspirate the supernatant, wash the cells twice, and add 4% paraformaldehyde to fix for 1 hour. After fixation, add 1μg / mL Nile red to each well and incubate in a 37°C, 5% CO2 incubator for 30 minutes, then observe and take photos under a fluorescence microscope, and finally use Image J software to semi-quantitatively analyze the fluorescence data of the photos.

[0045] According to the fluorescence data, the relative oil content of the blank group was set as 100%, and the data of other groups were normalized to obtain the relative oil content. The t-test was used for pairwise comparison, where *P<0.05 indicated that the difference was statistically significant, **P<0.01 indicated that the difference was statistically significant, and ***P<0.001 indicated that the difference was extremely statistically significant.

[0046] The results of the test are as follows Figure 1 The results showed that the control group had a statistically significant difference compared with the blank group, which indicated that LA treatment could effectively stimulate lipid production; the experimental group had a significant difference compared with the control group, which indicated that after the addition of Ecdoin to the experimental group, the lipid content was significantly reduced, indicating that Ecdoin had the effect of inhibiting LA-induced lipid production.

[0047] Experimental Example 2: Effect of Ecdoin on Lipid Production under the Stimulation of Arachidonic Acid (AA)

[0048] Group settings:

[0049] Blank group: no AA stimulation treatment, no sample treatment

[0050] Control group: AA stimulation treatment, no sample treatment

[0051] Experimental group: AA stimulation treatment, 0.05% ectoine treatment

[0052] Test method: Take SZ95 cells and culture them in a T75 culture flask with DMEM medium. When the cell density is about 80%, inoculate them in a 24-well plate with a coverslip and culture them at 37°C and 5% CO2. After 24 hours, aspirate the supernatant, treat each well according to the above group settings, set 3 replicate wells for each group, and take 5 photos of each well randomly. Incubate in a 37°C, 5% CO2 incubator for 72 hours. Aspirate the supernatant, wash the cells twice, and add 4% paraformaldehyde to fix for 1 hour. After fixation, add 1μg / mL Nile red to each well and incubate in a 37°C, 5% CO2 incubator for 30 minutes, then observe and take photos under a fluorescence microscope, and finally use Image J software to semi-quantitatively analyze the fluorescence data of the photos.

[0053] According to the fluorescence data, the relative oil content of the blank group was set as 100%, and the data of other groups were normalized to obtain the relative oil content. The t-test was used for pairwise comparison, *P<0.05 indicated that the difference was statistically significant, **P<0.01 indicated that the difference was statistically significant, and ***P<0.001 indicated that the difference was extremely statistically significant.

[0054] The results of the test are as follows Figure 2 The above results show that the control group has a statistical difference compared with the blank group, which indicates that AA treatment effectively stimulates lipid production; the experimental group has no significant difference compared with the control group, which indicates that after adding ectoine to the experimental group for co-incubation, there is no significant inhibition, indicating that the inhibitory effect of ectoine on AA-induced lipid production is not obvious.

Claims

1. Use of ectoine in inhibiting and / or reducing sebum production.

2. Use of ectoine as an oil-controlling active agent in the preparation of a composition for external use on the skin.

3. The use of Ecdoin as an oil control active agent.

4. The use according to any one of claims 1 to 3, wherein the ectoine is used to inhibit and / or reduce the sebum production caused by linoleic acid.

5. The use according to any one of claims 1-4, wherein the use of ectoine in preventing and / or alleviating excessive sebum secretion.

6. The use according to claim 5, wherein the ectoine is used to prevent and / or alleviate the formation of one or more of acne, pimples, acne, blackheads, inflammatory papules, pustules, enlarged pores, dull skin color, nodules, abscesses, and dandruff.

7. A method for suppressing and / or removing sebum, comprising applying ectoine to the skin.

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