Leukocyte extract-containing soothing anti-allergy essence and preparation method thereof

Abstract

The invention relates to the field of skin care products, in particular to soothing and anti-allergy essence containing a leukocyte extract and a preparation method of the soothing and anti-allergy essence. According to the formula proportion, the composition comprises the following components in percentage by weight: 1 to 75 percent of water, 0.3 to 0.5 percent of p-hydroxyacetophenone, 0.2 to 0.5 percent of an amino acid humectant, 0.1 to 0.5 percent of phenoxyethanol, 0.16 to 10 percent of a leukocyte extract, 0.1 to 1 percent of recombinant III type collagen, 0.5 to 10 percent of a mixed component I, 5 to 10 percent of a mixed component II, 0.1 to 2 percent of tetrahydromethylpyrimidine carboxylic acid and 0.2 to 1 percent of ergosulfide. The facial mask can relieve skin for a long time, promote collagen generation of the skin, and make the skin bright, fine, smooth and moisturized. All people can experience the new charm of gene skin care, and the young state of the skin is kept.

Description

Technical Field

[0001] The invention relates to the field of skin care products, and in particular to a soothing and anti-allergic essence containing leukocyte extract and a preparation method thereof. Background Art

[0002] Scientific experiments have shown that the occurrence of rough, dry, dehydrated and wrinkled skin is caused by the slowdown of skin metabolism and the accumulation and thickening of the stratum corneum. Dryness and dehydration are caused by the decrease of water content in the skin and the reduction of water retention. Wrinkles are caused by the slowdown of collagen fiber and elastic fiber production, the formation of gaps, and the dehydration of the skin. At the same time, the skin barrier will also be damaged. Once the sebum membrane is damaged, the moisturizing function is reduced, the skin becomes dry, itchy and even peeling, and the responsiveness to the climate is weakened, which can easily cause skin redness and swelling, local redness, and even sensitivity. It is particularly prone to pigmentation and dull skin color. Therefore, there is an urgent need for a soothing and anti-allergic essence to relieve the above skin problems. Summary of the invention

[0003] In view of the problems existing in the background technology, a soothing and anti-allergic essence containing leukocyte extract and a preparation method thereof are proposed.

[0004] The invention provides a soothing and anti-allergic essence containing leukocyte extract, which comprises, according to the formula ratio, 1-75% of water, 0.3-0.5% of p-hydroxyacetophenone, 0.2-0.5% of an amino acid moisturizer, 0.1-0.5% of phenoxyethanol, 0.16-10% of a leukocyte extract, 0.1-1% of recombinant type III collagen, 0.5-10% of a mixed component one, 5-10% of a mixed component two, 0.1-2% of ectoine and 0.2-1% of ergothioneine.

[0005] Preferably, mixed component one includes 0.46625-9.325% water, 0.0125-0.25% sodium hyaluronate cross-linked polymer, 0.0125-0.25% sodium hyaluronate, 0.00325-0.065% 1,3-propylene glycol, 0.0015-0.03% hydrolyzed sodium hyaluronate, 0.0015-0.03% 1,2-propylene glycol, 0.001-0.02% acetylated sodium hyaluronate, 0.0005-0.01% hyaluronic acid, 0.0005-0.01% hydrolyzed glycosaminoglycan, 0.00025-0.005% hydroxypropyltrimethylammonium chloride hyaluronic acid and 0.00025-0.005% caprylhydroxamic acid.

[0006] Preferably, the second mixed component comprises 1.5-3% of Centella asiatica extract, 1.5-3% of Portulaca oleracea extract and 2-4% of Aloe vera extract.

[0007] The present invention further provides a method for preparing a soothing and anti-allergic essence containing leukocyte extract, the steps of which are as follows:

[0008] S1. Take the leukocyte extract exosome raw material in the formula and set aside;

[0009] S2, taking water, p-hydroxyacetophenone and amino acid moisturizer, stirring and mixing them evenly to dissolve, adding leukocyte extract, stirring evenly and setting aside;

[0010] S3, taking the mixed component 1, adding the Centella asiatica extract, the Portulaca oleracea extract and the Aloe vera extract to the mixture and mixing evenly to form a moisturizing and repairing active substance for standby use;

[0011] S4, mixing recombinant type III collagen, ectoine and ergothioneine evenly to form a barrier repair composition for standby use;

[0012] S5, after mixing the moisturizing and repairing active substance and the repairing barrier composition, stir evenly, add to S2, stir evenly again, then add phenoxyethanol and stir evenly to prepare a semi-finished soothing and anti-sensitive leukocyte extract essence for standby use;

[0013] S6, inspect the semi-finished products, and after they are qualified, fill and bottle them according to the selected specifications to make the required essence products and finalize them;

[0014] S7, outer packaging, boxing, and warehousing.

[0015] Compared with the prior art, the present invention has the following beneficial technical effects: this essence carries a large amount of active anti-inflammatory factors, and uses active factor ingredients for cell repair. The repair and anti-sensitivity effects of the multiple sodium hyaluronate composition and the triple plant extract composition of Centella asiatica extract, Portulaca oleracea extract, and Aloe vera extract are then utilized, and the anti-aging effects of recombinant type III collagen, Ectoin and ergothioneine are combined to form a unique soothing, anti-sensitivity and moisturizing white blood cell slow essence. This essence is in line with the modern bio-gene skin care concept and releases new energy for ecological skin beauty. Cytokines repair problematic skin, gently care for and control water, and establish a microecological barrier for the skin system. Long-lasting soothing promotes skin collagen production, making the skin translucent, smooth and hydrated. Let everyone experience the new charm of genetic skin care and keep the skin young. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 A diagram showing the preparation method of the soothing and anti-allergic essence containing leukocyte extracts of the present invention;

[0017] Figure 2 The results of the skin tightening and anti-wrinkle efficacy test of the leukocyte extract samples;

[0018] Figure 3 Results of moisturizing effect test of leukocyte extract samples

[0019] Figure 4 The soothing effect test results of the leukocyte extract samples are shown;

[0020] Figure 5 It is the irritation test result of the leukocyte extract sample;

[0021] Figure 6 This is a schematic diagram of the results of Icodoxin reducing the photodamage of ultraviolet and visible light to DNA in cells;

[0022] Figure 7 This is a schematic diagram showing that ectoine can improve the thermal tolerance of cells;

[0023] Figure 8 This is a schematic diagram showing that ectoine can enhance the activity of Langerhans cells;

[0024] Fig. 9 This is a schematic diagram showing that ectoine can improve the thermal stability of Cu / Zn-SOD;

[0025] Fig.10 Schematic diagram of the effect of different concentrations of Ecdoin in repairing UV-induced cell damage;

[0026] Fig.11 Schematic diagram of the water-locking effect of Ekodoin;

[0027] Fig.12 This is a schematic diagram of the redness-reducing effect of Icodone. DETAILED DESCRIPTION

[0028] Embodiment 1

[0029] The invention discloses a soothing and anti-allergic essence containing leukocyte extract, which comprises, according to the formula ratio, 1-75% of water, 0.3-0.5% of p-hydroxyacetophenone, 0.2-0.5% of amino acid moisturizer, 0.1-0.5% of phenoxyethanol, 0.16-10% of leukocyte extract, 0.1-1% of recombinant type III collagen, 0.5-10% of mixed component one, 5-10% of mixed component two, 0.1-2% of ectoine and 0.2-1% of ergothioneine.

[0030] It should be further explained that the mixed component one includes 0.46625-9.325% of water, 0.0125-0.25% of sodium hyaluronate cross-linked polymer, 0.0125-0.25% of sodium hyaluronate, 0.00325-0.065% of 1,3-propylene glycol, 0.0015-0.03% of hydrolyzed sodium hyaluronate, 0.0015-0.03% of 1,2-propylene glycol, 0.001-0.02% of acetylated sodium hyaluronate, 0.0005-0.01% of hyaluronic acid, 0.0005-0.01% of hydrolyzed glycosaminoglycan, 0.00025-0.005% of hydroxypropyltrimethylammonium chloride hyaluronic acid and 0.00025-0.005% of caprylhydroxamic acid.

[0031] It should be further explained that the second mixed component includes 1.5-3% of Centella asiatica extract, 1.5-3% of Portulaca oleracea extract and 2-4% of Aloe vera extract.

[0032] Embodiment 2

[0033] like Figure 1 As shown, the present invention further proposes a method for preparing a soothing and anti-allergic essence containing leukocyte extract, the steps are as follows:

[0034] S1. Take the leukocyte extract exosome raw material in the formula and set aside;

[0035] S2, taking water, p-hydroxyacetophenone and amino acid moisturizer, stirring and mixing them evenly to dissolve, adding leukocyte extract, stirring evenly and setting aside;

[0036] S3, taking the mixed component 1, adding the Centella asiatica extract, the Portulaca oleracea extract and the Aloe vera extract to the mixture and mixing evenly to form a moisturizing and repairing active substance for standby use;

[0037] S4, mixing recombinant type III collagen, ectoine and ergothioneine evenly to form a barrier repair composition for standby use;

[0038] S5, after mixing the moisturizing and repairing active substance and the repairing barrier composition, stir evenly, add to S2, stir evenly again, then add phenoxyethanol and stir evenly to prepare a semi-finished soothing and anti-sensitive leukocyte extract essence for standby use;

[0039] S6, inspect the semi-finished products, and after they are qualified, fill and bottle them according to the selected specifications to make the required essence products and finalize them;

[0040] S7, outer packaging, boxing, and warehousing.

[0041] It should be further explained that the preparation process of leukocyte extract exosomes is as follows:

[0042] 1. Healthy blood:

[0043] (1) Select fresh anticoagulated blood from healthy livestock (pigs, buffaloes, goats).

[0044] (2) Ensure that the source is healthy, has passed quarantine, is free of contamination, and does not contain blood clots.

[0045] 2. Centrifugal separation:

[0046] (1) Send the blood into a centrifuge for centrifugation to separate mononuclear cells.

[0047] (2) The mononuclear cells obtained by centrifugation are cultured in a culture medium containing a cell culture additive.

[0048] (3) Attention should be paid to the speed control during centrifugation to prevent the cell structure from being broken.

[0049] 3. Cultivation:

[0050] (1) Place the culture flask in an incubator containing 5% CO2 and culture at 37°C.

[0051] (2) Replace the cell culture medium every 24 hours, discard the old cell culture medium, and add an equal amount of fresh culture medium and cell culture supplements.

[0052] 4. Centrifuge again:

[0053] (1) Mature mononuclear cells are extracted by centrifugation, and the leukocyte extract is collected and stored.

[0054] (2) Attention should be paid to the rotation speed control during centrifugation to prevent the cell structure from being broken.

[0055] 5. Filtering:

[0056] (1) Use a 0.22 micron filter to ensure that cell fragments and microorganisms are intercepted by the filter.

[0057] (2) The generation of bubbles needs to be avoided during the filtration process to prevent filter clogging and rupture.

[0058] (3) After filtration, the filtrate is tested to confirm the presence of active substances and the absence of microorganisms.

[0059] 6. Mixing:

[0060] (1) The filtered extract is mixed with other ingredients (tetrahydromethylpyrimidine carboxylic acid, butylene glycol, 1,2-hexanediol, and p-hydroxyacetophenone) to improve the stability and quality of the product.

[0061] (2) Use a magnetic stirrer to mix the leukocyte extract thoroughly.

[0062] (3) Ensure that the mixing process is carried out in a sterile environment and that the required ingredients are mixed in the correct proportions according to the formulation requirements and standard operating procedures.

[0063] 7. Quality inspection, product filling and packaging:

[0064] (1) Perform quality control tests on leukocyte extracts for bacteria, fungi, endotoxins, and mycoplasma. (2) Fill and seal the product under a sterile environment to prevent contamination of the product during the packaging process. (3) After the product is packaged, clearly mark the product information on the outer packaging, including production date, expiration date, production batch, etc.

[0065] 8. Storage:

[0066] (1) Store packaged products at appropriate temperatures to maintain product stability and shelf life.

[0067] (2) Conduct regular inventory counts and quality monitoring to ensure the quality of inventory products.

[0068] (3) Storage conditions should meet the requirements of the product, including temperature, humidity, light, etc., and should be monitored and recorded during storage.

[0069] 9. Detection experiment of exosomes extracted from leukocytes

[0070] 9.1 Firming and anti-wrinkle efficacy testing

[0071] Experimental steps: Weigh the sample using an electronic balance, pretreat the test substance, and prepare a mixed solution. Incubate in an incubator at 25°C for 15 minutes. Then add a certain amount of elastase with a pipette and continue incubating for 15 minutes. Use an enzyme-labeled analyzer to test the OD value of the elastase inhibition rate at a wavelength of 410nm. Record the data, calculate the inhibition rate and statistical difference P value.

[0072] Laboratory environment temperature: 15℃-30℃. Environment humidity: 20%-75%.

[0073] Experimental conclusion: The test results are as follows Figure 2 As shown, the inhibition rate of the test sample is better than (higher than) the inhibition rate of the blank control group and the statistical difference P value is <0.05, indicating that the difference is significant and the test sample has firming and anti-wrinkle effects.

[0074] 9.2 Moisturizing efficacy test

[0075] Experimental steps: Weigh the sample using an electronic balance. Pretreat the test sample; select a reagent with known hygroscopicity as the positive control. Place the sample and the positive control in a constant temperature and humidity chamber under certain temperature and humidity conditions. Weigh the sample using an electronic balance regularly. Record the data, calculate the moisture change rate Δm, and make a curve.

[0076] Laboratory environment temperature: 15℃-30℃. Environment humidity: 20%-75%.

[0077] Experimental conclusion: The test results are as follows Figure 3 As shown, the moisture change rate Δm curve of the test sample is smaller (better) than the moisture change rate Δm curve of the positive control, indicating that the test sample has a moisturizing effect.

[0078] 9.3 Soothing effect test

[0079] Experimental steps: Weigh the sample using an electronic balance. Pretreat the test sample, prepare sample tubes with different dilutions, and make parallel blank and positive control groups. Add the reactants and mix well, then place in a constant temperature water bath at 37°C for 40 minutes. Add a pH adjuster, place in a boiling water bath for 15 minutes, ice bath for 10 minutes, and cool to room temperature. Use an enzyme-labeled analyzer to measure the OD value at a wavelength of 530nm, and calculate the hyaluronidase activity inhibition rate and statistical difference P value.

[0080] Laboratory environment temperature: 15℃-30℃. Environment humidity: 20%-75%.

[0081] Experimental conclusion: The test results are as follows Figure 4 As shown, the inhibition rate of the test sample is better than (higher than) the inhibition rate of the blank control group and the statistical difference P value is <0.05, indicating that the difference is significant and the test sample has a soothing effect.

[0082] 9.4 Irritation efficacy test

[0083] Experimental steps: Chicken embryo preparation, test samples are weighed with an electronic balance and pre-treated. Select chicken embryos with well-developed blood vessels, peel off the eggshell part of the air chamber and remove the eggshell membrane to ensure that the exposed chorioallantoic membrane (CAM) is intact. Take the test substance, negative control reagent and positive control reagent directly to act on the chorioallantoic membrane (CAM). After 5 minutes of action, immediately observe and take pictures under a stereo microscope and record. Give a score (ES) for the degree of change of each toxic effect, and calculate the average score.

[0084] The negative control reagent for this test is: 0.9% sodium chloride solution, and the positive control reagent is: 0.1 mol / L sodium hydroxide solution

[0085] Laboratory environment temperature: 18℃-25℃. Environment humidity: 30%-70%.

[0086] Table 1 Average ES scores of test samples

[0087]

[0088] Experimental conclusion: The test results are as follows Figure 5 As shown, combined with the grading indicators in Table 1, it can be seen that the test sample is mild (non-irritating).

[0089] Leukocyte exosomes are tiny membrane vesicles secreted by cells with a lipid bilayer membrane structure, a diameter of about 30-150 nanometers, and contain various bioactive molecules such as proteins, lipids, and nucleic acids. Exosomes transmit information between cells, regulate the phenotype and function of cells, and participate in a variety of physiological and pathological processes. In the skin immune system, exosomes play an important role in balancing the protection of the body from external threats and avoiding excessive inflammatory responses. Exosomes secreted by leukocytes can affect the activity of other immune cells or non-immune cells, regulate skin immune responses, and maintain the health of the skin. In addition, exosomes secreted by leukocytes can also promote angiogenesis required for the nutrient supply of skin cell growth, inhibit excessive inflammatory responses, and inhibit scar formation, which is of great significance to skin health.

[0090] The interleukins, interferons and perforins contained in the leukocyte extract can activate the immune function of the vaginal epidermal mucosa, activate the CIK immune cells and NK natural killer cells in the epidermal mucosal tissue, effectively improve the immune cells' killing ability against HPV viruses, bacteria and fungi, and prevent and reduce the risk of HPV infection and microbial infection in the vagina.

[0091] Ergothioneine is a powerful natural antioxidant that can be found in animal semen and a variety of medicinal plants. It has antioxidant effects and can improve skin condition, protect cells, scavenge free radicals, and moisturize.

[0092] It should be further explained that the three-dimensional matrix composed of sodium hyaluronate cross-linked polymer, sodium hyaluronate, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate, hyaluronic acid, hydrolyzed glycosaminoglycan and hydroxypropyltrimethylammonium chloride hyaluronic acid and its derivatives has a precise layered effect, which can replenish and lock water. The time-sharing penetration release can instantly sense the effect and can be maintained for a long time. It penetrates deep into the skin, revitalizes the skin's original power and resists the erosion of time.

[0093] It needs to be further explained that if Figure 6 As shown, the experiment found that ectoine, also known as icodone, can significantly reduce the photodamage of ultraviolet and visible light to DNA in cells. The visible light protection rate of DNA by 0.1mM (0.0014%) icodone reaches 92.7%, and the UVA / visible light protection rate reaches 68.9%.

[0094] Heat shock protein (HSP) is a protective protein synthesized when the body's cells are attacked by harsh environments such as high temperature, helping cells maintain normal physiological activities. When cells are stimulated by heat, the expression of HSP increases rapidly in a short period of time and then gradually returns to normal levels. The higher the expression of HSP, the more obvious the stimulation. Figure 7 As shown, after treatment with ectoine, the thermal tolerance of cells was improved, and the expression level of HSP72 was significantly reduced compared with the positive control group.

[0095] Dendritic cells are known as the immune barrier of the skin. According to their different distribution locations in the skin, they can be divided into Langerhans cells in the epidermis and dendritic cells in the dermis. In normal skin, Langerhans cells can capture antigens and selectively induce the activation and proliferation of skin resident regulatory cells to maintain normal skin immune tolerance. Figure 8 As shown in the figure, ultraviolet irradiation damages Langerhans cells and reduces their activity. After treatment with Ecdoin, the damage of Langerhans cells to ultraviolet light was significantly reduced. Compared with the positive control group, 1% Ecdoin can increase cell activity by 10.5% after 6 hours.

[0096] SOD enzyme, or superoxide dismutase, is a metal enzyme that can remove superoxide free radical anions from the body and effectively prevent the toxic effects of superoxide free radical anions on the body. As a biological macromolecule, the natural SOD enzyme has different metal cofactors and different stability. CuIZn-SOD has poor thermal stability and is easily inactivated. Fig. 9 As shown, the experiment found that ectoine can significantly improve the thermal stability of Cu / Zn-SOD at extremely low concentrations.

[0097] Human immortalized epidermal keratinocytes HaCaT were irradiated with certain doses of UVA and UVB, and different concentrations of ectoine were added to treat the cells after irradiation. Fig.10 As shown, the test results show that Ecdoin can significantly repair cell damage caused by UV. 0.5% Ecdoin can restore cell activity to 89.2%, and 2% Ecdoin can restore it to 98.7%, which can almost eliminate the damage caused by UV to cells.

[0098] The cyclized structure of the Ecdoin molecule makes its surface charge densely distributed, forming a hydrophilic electron cloud. Therefore, Ecdoin has excellent water binding ability. Fig.11 As shown in the figure, the water molecule clusters are tightly associated around the ectoine molecules, and the structure is stable. As time goes by, the number of water molecules will not decrease. However, the number of water molecules around the glycerol molecules decreases sharply as time goes by. This shows that ectoine can strengthen the hydrogen bonding between water molecules.

[0099] like Fig.12As shown, A1 is a 1% ectoine solution and A2 is a 1% dipotassium glycyrrhizinate solution. Ectoine can effectively reduce facial erythema and soothe sensitive skin, and its effect is better than dipotassium glycyrrhizinate at the same concentration.

[0100] Therefore, the soothing and anti-allergic essence of the present invention is suitable for the following groups of people:

[0101] 1. Hormone face caused by long-term use of inferior cosmetics containing hormones, or frequent use of skin care products of different brands, which causes damage to the skin's stratum corneum and corneal layer.

[0102] 2. Sensitive skin, thin skin stratum corneum, sensitive skin with lost protective barrier

[0103] 3. Long-term dryness, roughness, shrinkage, collapse, dullness, inelasticity, wrinkles, collagen loss, dehydration and aging of the skin.

[0104] 4. People with acne-prone skin who stay up late for a long time, have an irregular lifestyle, and frequently get blackheads and pimples on their faces.

[0105] 5. Medical beauty hospitals, institutions, and post-operative repair after minimally invasive surgery.

[0106] Scientific experiments have proven that rough, dry, dehydrated and wrinkled skin is caused by the slowdown of skin metabolism and the accumulation and thickening of the stratum corneum. Dryness and dehydration are caused by the decrease of water content in the skin and the reduction of water retention. Wrinkles are caused by the slowdown of collagen fiber and elastic fiber production, the formation of gaps, and the dehydration of the skin. At the same time, the skin barrier will also be damaged. Once the sebum membrane is damaged, the moisturizing function will be reduced, the skin will become dry, itchy and even peeling, and the responsiveness to the climate will be weakened, which can easily cause skin redness and swelling, local redness, and even sensitivity. It is particularly prone to pigmentation and dull skin color.

[0107] This essence contains a large number of active anti-inflammatory factors and uses active factor ingredients for cell repair. The repair and anti-sensitivity effects of the triple plant extract composition of sodium hyaluronate and Centella asiatica extract, Portulaca oleracea extract, and Aloe vera extract, plus the anti-aging effects of collagen, ectoine, and ergothioneine, are combined into a unique soothing, anti-sensitivity, and moisturizing white blood cell slow essence. This essence is in line with the modern bio-genetic skin care concept and releases new energy for ecological skin beauty. Cytokines repair problematic skin, gently care for and control water, and establish a microecological barrier for the skin system. Long-lasting soothing promotes the production of skin collagen, making the skin translucent, smooth, and hydrated. Let everyone experience the new charm of genetic skin care and keep the skin young.

[0108] The embodiments of the present invention are described in detail above with reference to the accompanying drawings, but the present invention is not limited thereto, and various changes can be made within the knowledge scope of technicians in the relevant technical field without departing from the purpose of the present invention.

Claims

1. A soothing and anti-allergic essence containing leukocyte extract, characterized in that: According to the formula proportion, the invention comprises 1-75% of water, 0.3-0.5% of p-hydroxyacetophenone, 0.2-0.5% of amino acid moisturizer, 0.1-0.5% of phenoxyethanol, 0.16-10% of leukocyte extract, 0.1-1% of collagen, 0.5-10% of mixed component one, 5-10% of mixed component two, 0.1-2% of tetrahydromethylpyrimidine carboxylic acid and 0.2-1% of ergothioneine.

2. The soothing and anti-allergic essence containing leukocyte extract according to claim 1, characterized in that: Mixed component one includes 0.46625-9.325% water, 0.0125-0.25% sodium hyaluronate cross-linked polymer, 0.0125-0.25% sodium hyaluronate, 0.00325-0.065% 1,3-propylene glycol, 0.0015-0.03% hydrolyzed sodium hyaluronate, 0.0015-0.03% 1,2-propylene glycol, 0.001-0.02% acetylated sodium hyaluronate, 0.0005-0.01% hyaluronic acid, 0.0005-0.01% hydrolyzed glycosaminoglycan, 0.00025-0.005% hydroxypropyltrimethylammonium chloride hyaluronic acid and 0.00025-0.005% caprylhydroxamic acid.

3. The soothing and anti-allergic essence containing leukocyte extract according to claim 2, characterized in that: The second mixed component comprises 1.5-3% of Centella asiatica extract, 1.5-3% of Portulaca oleracea extract and 2-4% of Aloe vera extract.

4. The method for preparing the soothing and anti-allergic essence containing leukocyte extract according to claim 3, characterized in that: Here are the steps: S1. Take the leukocyte extract exosome raw material in the formula and set aside; S2, taking water, p-hydroxyacetophenone and amino acid moisturizer, stirring and mixing them evenly to dissolve, adding leukocyte extract, stirring evenly and setting aside; S3, taking the mixed component 1, adding the Centella asiatica extract, the Portulaca oleracea extract and the Aloe vera extract to the mixture and mixing evenly to form a moisturizing and repairing active substance for standby use; S4, mixing recombinant type III collagen, ectoine and ergothioneine evenly to form a barrier repair composition for standby use; S5, after mixing the moisturizing and repairing active substance and the repairing barrier composition, stir evenly, add to S2, stir evenly again, then add phenoxyethanol and stir evenly to prepare a semi-finished soothing and anti-sensitive leukocyte extract essence for standby use; S6, inspect the semi-finished products, and after they pass the inspection, fill and bottle them according to the selected specifications to make the required essence products and finalize them; S7, outer packaging, boxing, and warehousing.

Images