Eye protection liquid based on natural extracts and preparation method thereof

Abstract

The invention discloses eye protection liquid based on natural extracts and a preparation method thereof, and belongs to the technical field of eye protection liquid, the eye protection liquid is prepared from the following raw materials in parts by weight: 100-200 parts of 0.9% NaCl solution, 12-14 parts of sodium hyaluronate, 1-3 parts of xanthophyll, 0.1-1 part of natural borneol micro powder, 16-25 parts of cassia seed and wild chrysanthemum flower fermentation extract, 17-22 parts of eggshell membrane extract and 6-10 parts of rosa laevigata michx root triterpene. According to the mode, the eye protection liquid is prepared from the sodium hyaluronate, the xanthophyll, the natural borneol micro powder, the cassia seed and wild chrysanthemum flower aqueous extract, the eggshell membrane extract and the rosa laevigata michx root triterpenoid, the raw materials are few in variety, alcohol is not contained, irritation is low, the eye protection liquid is suitable for people with sensitive eyes, the use method is simple, and xerophthalmia can be effectively improved.

Description

Technical Field

[0001] The present invention relates to the technical field of eye protection liquid, and in particular to an eye protection liquid based on natural extracts and a preparation method thereof. Background Art

[0002] Eye protection solution can be used when you overuse your eyes due to prolonged use of electronic products, study, work, etc. It can also be used as a daily care product for those wearing cosmetic lenses, contact lenses, etc., and can effectively relieve eye fatigue.

[0003] In order to alleviate the side effects caused by long-term use of eye drops containing chemical ingredients, some purely plant-based eye protection solutions have appeared on the market. For example, Chinese invention patent CN111558031B discloses a purely plant-based multifunctional eye nutrient solution and its preparation method, which includes the following raw materials: angelica peptide, ginseng peptide, sea cucumber peptide, bitter melon peptide, astragalus, rhodiola rosea, houttuynia cordata, peach kernel, safflower, kudzu root, adenophora, Scrophularia ningpoensis, salvia miltiorrhiza, wine rhubarb, herba schizonepetae and mother of pearl.

[0004] However, it has the following problems: 1) There are many types of raw materials, which are irritating to the eyes and are not suitable for people with sensitive eyes (such as children and the elderly); 2) It uses 55°C high-proof grain wine to turn borneol into liquid. The addition of alcohol will further increase the irritation of the product and even cause allergic reactions.

[0005] Based on this, the present invention designs an eye protection solution based on natural extracts and a preparation method thereof to solve the above problems. Summary of the Invention

[0006] In view of the above-mentioned shortcomings of the prior art, the present invention provides an eye protection solution based on natural extracts and a preparation method thereof.

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions: An eye protection solution based on natural extracts, comprising the following raw materials in parts by weight: 100-200 parts of 0.9% NaCl solution, 12-14 parts of sodium hyaluronate, 1-3 parts of lutein, 0.1-1 parts of natural borneol powder, 16-25 parts of fermented extracts of cassia seeds and wild chrysanthemums, 17-22 parts of eggshell membrane extract, and 6-10 parts of triterpenes from the root of Rosa laevigata; The method for preparing the eggshell membrane extract comprises the following steps: A. Collect eggshell membranes, crush them, add 2-3 times the volume of 0.6% saline, and soak for 15-20 minutes; B. After removing the eggshell membrane powder, immerse it in an acetic anhydride solution for 4-6 minutes; then immerse it in a gelatinized starch solution containing a coupling agent, and irradiate it at a dose of 0.35-0.42 kGy for 10-15 minutes. C. Filter and remove the eggshell membrane powder, wash with water 1-3 times, and first hydrolyze with pepsin (pH 2, 37°C, 5 hours), then with alkaline protease (pH 9, 53°C, 1.5 hours), using an enzyme dosage of 7-8 U / mg. D. Ultrafiltration to obtain ultrafiltration separation components with a molecular weight of 5~10kDa.

[0008] Furthermore, the eye protection solution is packaged in a spray bottle.

[0009] Furthermore, the mass ratio of cassia seeds to wild chrysanthemum in the fermentation extract is 1.5-2:1.

[0010] Furthermore, the particle size of the natural borneol powder is nanometer level.

[0011] Furthermore, the concentration of the acetic anhydride solution is 1-1.3 mol / L.

[0012] Furthermore, the coupling agent is an amino-terminated silane coupling agent.

[0013] Furthermore, the gelatinized starch solution is prepared by mixing gelatinized starch with 4 to 8 times the volume of water.

[0014] Furthermore, the method for preparing the extract from fermentation of Cassia seeds and wild chrysanthemum is as follows: A. Wash Cassia seeds and wild chrysanthemums, dry them in the sun, freeze them below -25°C, and grind them into mixed plant powder. B. Add water and yeast to the plant powder mixture, mix well, ferment at 25-30°C for 4-6 hours, then steam at 100-105°C for 5-8 minutes, remove from the mixture and cool to 35-38°C while stirring, then add lactic acid bacteria, mix well, ferment at 35-38°C for 1-2 hours, then steam at 95-100°C for 1-3 minutes; C. After centrifugation, ultrasonic-assisted extraction was performed at an ultrasonic power of 300-400 W and an ultrasonic frequency of 30-40 kHz for 25-30 min, followed by microfiltration using a membrane pore size of 0.6-0.8 μm to obtain a filtrate; D. Adjust the pH of the filtrate to 7-7.2, extract with an equal volume of ethanol, recover the ethanol by distillation under reduced pressure, and then filter with a filter membrane with a pore size of 0.5-0.7 μm to obtain a clear liquid, which is the fermentation extract of Cassia seed and wild chrysanthemum.

[0015] Furthermore, the triterpenoid compound of the root of Rosa laevigata is Rosa laevigata sapogenin.

[0016] A method for preparing an eye protection solution based on natural extracts comprises the following steps: adding sodium hyaluronate, lutein, natural borneol powder, fermented extracts of cassia seeds and wild chrysanthemums, eggshell membrane extract, and triterpenes from the root of Rosa laevigata to a 0.9% NaCl solution, stirring uniformly at 2000-3000 r / min, and sterilizing to obtain the eye protection solution based on natural extracts.

[0017] In order to better achieve the purpose of the present invention, the present invention also provides an eye protection solution based on natural extracts and a preparation method thereof.

[0018] Compared with the prior art, the present invention has the following beneficial effects: the eye protection liquid of the present invention is prepared using sodium hyaluronate, lutein, natural borneol powder, cassia seed and wild chrysanthemum water extract, eggshell membrane extract, and Rosa laevigata root triterpenoids. It has fewer types of raw materials, does not contain alcohol, has low irritation, is suitable for people with sensitive eyes, is simple to use, and can effectively improve dry eye syndrome. DETAILED DESCRIPTION

[0019] To make the purpose, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0020] Example 1: In some embodiments, a method for preparing an eye protection solution based on natural extracts comprises the following steps: 1. Preparation of eggshell membrane extract: A. Collect eggshell membranes, crush them, add 2 volumes of 0.6% saline, and soak for 20 minutes; B. The eggshell membrane powder was removed and immersed in an acetic anhydride solution (1 mol / L) for 6 minutes. The eggshell membrane powder was then immersed in a gelatinized starch solution (prepared by mixing gelatinized starch with 4 volumes of water) containing a coupling agent (amino-terminated silane coupling agent) and irradiated at a dose of 0.42 kGy for 10 minutes. C. Filter and remove the eggshell membrane powder, wash once with water, and enzymatically hydrolyze with pepsin (pH 2, 37°C, 5 hours), then with alkaline protease (pH 9, 53°C, 1.5 hours), using an enzyme dosage of 8 U / mg. D. Ultrafiltration to obtain ultrafiltration separation components with a molecular weight of 5~10kDa.

[0021] 2. Preparation of fermentation and extract of Cassia seed and wild chrysanthemum: A. Wash Cassia seeds and wild chrysanthemums, dry them in the sun, freeze them below -25°C, and grind them into mixed plant powder. B. Add water and yeast to the plant powder mixture, mix well, ferment at 25°C for 6 hours, then steam at 100°C for 8 minutes, remove from the mixture and cool to 35°C while stirring, then add lactic acid bacteria, mix well, ferment at 35°C for 1 hour, then steam at 100°C for 1 minute; C. Ultrasonic assisted extraction after centrifugation, with an ultrasonic power of 300 W and an ultrasonic frequency of 40 kHz for 25 min, followed by microfiltration using a filter membrane with a pore size of 0.8 μm to obtain a filtrate; D. Adjust the pH of the filtrate to 7, extract with an equal volume of ethanol, recover the ethanol by distillation under reduced pressure, and then filter with a filter membrane with a pore size of 0.7 μm to obtain a clear liquid, which is the fermentation extract of Cassia seed and wild chrysanthemum.

[0022] 3. Weigh the raw materials: 100 g of 0.9% NaCl solution, 14 g of sodium hyaluronate, 1 g of lutein, 1 g of natural borneol powder (nanoscale), 16 g of fermented extract of cassia seed and wild chrysanthemum (the mass ratio of cassia seed: wild chrysanthemum is 2:1), 22 g of eggshell membrane extract, 6 g of triterpenoids from the root of Rosa laevigata (Rosa laevigata sapogenin, for preparation method, refer to 201810455214.6).

[0023] 4. Add sodium hyaluronate, lutein, natural borneol powder, fermentation extract of cassia seed and wild chrysanthemum, eggshell membrane extract, and triterpenoids of Rosa laevigata root to 0.9% NaCl solution, stir evenly at 2000 r / min, and sterilize to obtain eye protection solution based on natural extracts.

[0024] Example 2: In some embodiments, a method for preparing an eye protection solution based on natural extracts comprises the following steps: 1. Preparation of eggshell membrane extract: A. Collect eggshell membranes, crush them, add 3 times the volume of 0.6% saline, and soak for 15 minutes; B. The eggshell membrane powder was removed and immersed in an acetic anhydride solution (1.3 mol / L) for 4 minutes. The eggshell membrane powder was then immersed in a gelatinized starch solution (prepared by mixing gelatinized starch with 8 volumes of water) containing a coupling agent (amino-terminated silane coupling agent) and irradiated at a dose of 0.35 kGy for 15 minutes. C. Filter and remove the eggshell membrane powder, wash it three times with water, and then enzymatically hydrolyze it with pepsin (pH 2, 37°C, 5 hours), and then with alkaline protease (pH 9, 53°C, 1.5 hours), using an enzyme dosage of 7 U / mg. D. Ultrafiltration to obtain ultrafiltration separation components with a molecular weight of 5~10kDa.

[0025] 2. Preparation of fermentation and extract of Cassia seed and wild chrysanthemum: A. Wash Cassia seeds and wild chrysanthemums, dry them in the sun, freeze them below -25°C, and grind them into mixed plant powder. B. Add water and yeast to the plant powder mixture, mix well, ferment at 30°C for 4 hours, then steam at 105°C for 5 minutes, remove from the mixture and cool to 38°C while stirring, then add lactic acid bacteria, mix well, ferment at 38°C for 2 hours, then steam at 95°C for 3 minutes; C. After centrifugation, ultrasonic-assisted extraction was performed at an ultrasonic power of 400 W, an ultrasonic frequency of 30 kHz, and a time of 30 min, followed by microfiltration using a filter membrane with a pore size of 0.6 μm to obtain a filtrate; D. Adjust the pH of the filtrate to 7.2, extract with an equal volume of ethanol, recover the ethanol by distillation under reduced pressure, and then filter with a filter membrane with a pore size of 0.5 μm to obtain a clear liquid, which is the fermentation extract of Cassia seed and wild chrysanthemum.

[0026] 3. Weigh the raw materials: 200 g of 0.9% NaCl solution, 12 g of sodium hyaluronate, 3 g of lutein, 0.1 g of natural borneol powder (nanoscale), 25 g of fermented extract of cassia seed and wild chrysanthemum (the mass ratio of cassia seed: wild chrysanthemum is 1.5:1), 17 g of eggshell membrane extract, 10 g of triterpenoids from the root of Rosa laevigata (Rosa laevigata sapogenin, for preparation method, refer to 201810455214.6).

[0027] 4. Add sodium hyaluronate, lutein, natural borneol powder, fermentation extract of cassia seed and wild chrysanthemum, eggshell membrane extract, and triterpenoids of Rosa laevigata root to 0.9% NaCl solution, stir evenly at 3000 r / min, and sterilize to obtain eye protection solution based on natural extracts.

[0028] Example 3: In some embodiments, a method for preparing an eye protection solution based on natural extracts comprises the following steps: 1. Preparation of eggshell membrane extract: A. Collect eggshell membranes, crush them, add 2.2 times the volume of 0.6% saline, and soak for 16 minutes; B. The eggshell membrane powder was removed and immersed in an acetic anhydride solution (1.1 mol / L) for 5 minutes. The eggshell membrane powder was then immersed in a gelatinized starch solution (prepared by mixing gelatinized starch with 5 volumes of water) containing a coupling agent (amino-terminated silane coupling agent) and irradiated at a dose of 0.38 kGy for 11 minutes. C. Filter and remove the eggshell membrane powder, wash twice with water, and enzymatically hydrolyze with pepsin (pH 2, 37°C, 5 hours), then with alkaline protease (pH 9, 53°C, 1.5 hours), using an enzyme dosage of 7.5 U / mg. D. Ultrafiltration to obtain ultrafiltration separation components with a molecular weight of 5~10kDa.

[0029] 2. Preparation of fermentation and extract of Cassia seed and wild chrysanthemum: A. Wash Cassia seeds and wild chrysanthemums, dry them in the sun, freeze them below -25°C, and grind them into mixed plant powder. B. Add water and yeast to the plant powder mixture, mix well, ferment at 26°C for 5 hours, then steam at 102°C for 6 minutes, remove from the mixture and cool to 36°C while stirring, then add lactic acid bacteria, mix well, ferment at 36°C for 1.5 hours, then steam at 98°C for 2 minutes; C. Ultrasonic assisted extraction after centrifugation, with an ultrasonic power of 350 W and an ultrasonic frequency of 33 kHz for 26 min, followed by microfiltration using a membrane with a pore size of 0.7 μm to obtain a filtrate; D. Adjust the pH of the filtrate to 7.1, extract with an equal volume of ethanol, recover the ethanol by distillation under reduced pressure, and then filter with a filter membrane with a pore size of 0.6 μm to obtain a clear liquid, which is the fermentation extract of Cassia seed and wild chrysanthemum.

[0030] 3. Weigh the raw materials: 120 g of 0.9% NaCl solution, 13 g of sodium hyaluronate, 1.3 g of lutein, 0.2 g of natural borneol powder (nanoscale), 18 g of fermented extract of cassia seed and wild chrysanthemum (the mass ratio of cassia seed: wild chrysanthemum is 1.8:1), 18 g of eggshell membrane extract, 7 g of triterpenoids from the root of Rosa laevigata (Rosa laevigata sapogenin, for preparation method, see 201810455214.6).

[0031] 4. Add sodium hyaluronate, lutein, natural borneol powder, fermentation extract of cassia seed and wild chrysanthemum, eggshell membrane extract, and triterpenoids of Rosa laevigata root to 0.9% NaCl solution, stir evenly at 2500r / min, and sterilize to obtain eye protection solution based on natural extracts.

[0032] Example 4: In some embodiments, a method for preparing an eye protection solution based on natural extracts comprises the following steps: 1. Preparation of eggshell membrane extract: A. Collect eggshell membranes, crush them, add 2 volumes of 0.6% saline, and soak for 18 minutes; B. The eggshell membrane powder was removed and immersed in an acetic anhydride solution (1 mol / L) for 6 minutes. The eggshell membrane powder was then immersed in a gelatinized starch solution (prepared by mixing gelatinized starch with 7 volumes of water) containing a coupling agent (amino-terminated silane coupling agent) and irradiated at a dose of 0.40 kGy for 13 minutes. C. Filter and remove the eggshell membrane powder, wash it three times with water, and then enzymatically hydrolyze it with pepsin (pH 2, 37°C, 5 hours), and then with alkaline protease (pH 9, 53°C, 1.5 hours), using an enzyme dosage of 7 U / mg. D. Ultrafiltration to obtain ultrafiltration separation components with a molecular weight of 5~10kDa.

[0033] 2. Preparation of fermentation and extract of Cassia seed and wild chrysanthemum: A. Wash Cassia seeds and wild chrysanthemums, dry them in the sun, freeze them below -25°C, and grind them into mixed plant powder. B. Add water and yeast to the plant powder mixture, mix well, ferment at 29°C for 5 hours, then steam at 104°C for 7 minutes, remove from the mixture and cool to 37°C while stirring, then add lactic acid bacteria, mix well, ferment at 37°C for 1 hour, then steam at 99°C for 2 minutes; C. Ultrasonic assisted extraction after centrifugation, with an ultrasonic power of 340 W and an ultrasonic frequency of 35 kHz for 29 min, followed by microfiltration using a membrane pore size of 0.7 μm to obtain a filtrate; D. Adjust the pH of the filtrate to 7, extract with an equal volume of ethanol, recover the ethanol by distillation under reduced pressure, and then filter with a filter membrane with a pore size of 0.6 μm to obtain a clear liquid, which is the fermentation extract of Cassia seed and wild chrysanthemum.

[0034] 3. Weigh the raw materials: 140 g of 0.9% NaCl solution, 13.5 g of sodium hyaluronate, 2.2 g of lutein, 0.3 g of natural borneol powder (nanoscale), 19 g of fermented extract of cassia seed and wild chrysanthemum (the mass ratio of cassia seed: wild chrysanthemum is 1.8:1), 20 g of eggshell membrane extract, 8 g of triterpenoids from the root of Rosa laevigata (Rosa laevigata sapogenin, for preparation method, see 201810455214.6).

[0035] 4. Add sodium hyaluronate, lutein, natural borneol powder, fermentation and extract of cassia seed and wild chrysanthemum, eggshell membrane extract, and triterpenoids of Rosa laevigata root to 0.9% NaCl solution, stir evenly at 2700 r / min, and sterilize to obtain eye protection solution based on natural extracts.

[0036] Example 5: In some embodiments, a method for preparing an eye protection solution based on natural extracts comprises the following steps: 1. Preparation of eggshell membrane extract: A. Collect eggshell membranes, crush them, add 3 times the volume of 0.6% saline, and soak for 19 minutes; B. The eggshell membrane powder was removed and immersed in an acetic anhydride solution (1 mol / L) for 4.5 minutes. The eggshell membrane powder was then immersed in a gelatinized starch solution (prepared by mixing gelatinized starch with 7 volumes of water) containing a coupling agent (amino-terminated silane coupling agent) and irradiated at a dose of 0.41 kGy for 13 minutes. C. Filter and remove the eggshell membrane powder, wash twice with water, and enzymatically hydrolyze with pepsin (pH 2, 37°C, 5 hours), then with alkaline protease (pH 9, 53°C, 1.5 hours), using an enzyme dosage of 7.5 U / mg. D. Ultrafiltration to obtain ultrafiltration separation components with a molecular weight of 5~10kDa.

[0037] 2. Preparation of fermentation and extract of Cassia seed and wild chrysanthemum: A. Wash Cassia seeds and wild chrysanthemums, dry them in the sun, freeze them below -25°C, and grind them into mixed plant powder. B. Add water and yeast to the plant powder mixture, mix well, ferment at 26°C for 5 hours, then steam at 103°C for 7 minutes, remove from the mixture and cool to 35.5°C while stirring, then add lactic acid bacteria, mix well, ferment at 35.5°C for 1.6 hours, then steam at 98°C for 2 minutes; C. Ultrasonic assisted extraction after centrifugation, with an ultrasonic power of 380 W and an ultrasonic frequency of 39 kHz for 27 min, followed by microfiltration using a membrane with a pore size of 0.7 μm to obtain a filtrate; D. Adjust the pH of the filtrate to 7.2, extract with an equal volume of ethanol, recover the ethanol by distillation under reduced pressure, and then filter with a filter membrane with a pore size of 0.6 μm to obtain a clear liquid, which is the fermentation and extract of Cassia seed and wild chrysanthemum.

[0038] 3. Weigh the raw materials: 170 g of 0.9% NaCl solution, 13 g of sodium hyaluronate, 2.3 g of lutein, 0.7 g of natural borneol powder (nanoscale), 22 g of fermented extract of cassia seed and wild chrysanthemum (the mass ratio of cassia seed: wild chrysanthemum is 1.8:1), 21 g of eggshell membrane extract, 8 g of triterpenoids from the root of Rosa laevigata (Rosa laevigata sapogenin, for preparation method, see 201810455214.6).

[0039] 4. Add sodium hyaluronate, lutein, natural borneol powder, fermentation extract of cassia seed and wild chrysanthemum, eggshell membrane extract, and triterpenoids of Rosa laevigata root to 0.9% NaCl solution, stir evenly at 2600 r / min, and sterilize to obtain eye protection solution based on natural extracts.

[0040] Comparative Example 1: The difference from Example 5 is that the eggshell membrane extract is directly subjected to enzymatic hydrolysis during preparation. The preparation method comprises the following steps: 1. Preparation of eggshell membrane extract: A. Collect eggshell membranes, crush them, and wash them twice with water. Hydrolyze them first with pepsin (pH 2, 37°C, 5 hours), then with alkaline protease (pH 9, 53°C, 1.5 hours), using 7.5 U / mg of enzyme. B. Ultrafiltration to obtain ultrafiltration separation components with a molecular weight of 5~10kDa.

[0041] 2. Preparation of fermentation and extract of Cassia seed and wild chrysanthemum: A. Wash Cassia seeds and wild chrysanthemums, dry them in the sun, freeze them below -25°C, and grind them into mixed plant powder. B. Add water and yeast to the plant powder mixture, mix well, ferment at 26°C for 5 hours, then steam at 103°C for 7 minutes, remove from the mixture and cool to 35.5°C while stirring, then add lactic acid bacteria, mix well, ferment at 35.5°C for 1.6 hours, then steam at 98°C for 2 minutes; C. Ultrasonic assisted extraction after centrifugation, with an ultrasonic power of 380 W and an ultrasonic frequency of 39 kHz for 27 min, followed by microfiltration using a membrane with a pore size of 0.7 μm to obtain a filtrate; D. Adjust the pH of the filtrate to 7.2, extract with an equal volume of ethanol, recover the ethanol by distillation under reduced pressure, and then filter with a filter membrane with a pore size of 0.6 μm to obtain a clear liquid, which is the fermentation and extract of Cassia seed and wild chrysanthemum.

[0042] 3. Weigh the raw materials: 170 g of 0.9% NaCl solution, 13 g of sodium hyaluronate, 2.3 g of lutein, 0.7 g of natural borneol powder (nanoscale), 22 g of fermented extract of cassia seed and wild chrysanthemum (the mass ratio of cassia seed: wild chrysanthemum is 1.8:1), 21 g of eggshell membrane extract, 8 g of triterpenoids from the root of Rosa laevigata (Rosa laevigata sapogenin, for preparation method, see 201810455214.6).

[0043] 4. Add sodium hyaluronate, lutein, natural borneol powder, fermentation extract of cassia seed and wild chrysanthemum, eggshell membrane extract, and triterpenoids of Rosa laevigata root to 0.9% NaCl solution, stir evenly at 2600 r / min, and sterilize to obtain eye protection solution based on natural extracts.

[0044] Comparative Example 2: The difference from Example 5 is that the fermentation and extract of Cassia seed and Chrysanthemum indica is replaced by the Cassia seed and Chrysanthemum indica water extract obtained by conventional water extraction method.

[0045] A method for preparing an eye protection solution based on natural extracts comprises the following steps: 1. Preparation of eggshell membrane extract: A. Collect eggshell membranes, crush them, add 3 times the volume of 0.6% saline, and soak for 19 minutes; B. The eggshell membrane powder was removed and immersed in an acetic anhydride solution (1 mol / L) for 4.5 minutes. The eggshell membrane powder was then immersed in a gelatinized starch solution (prepared by mixing gelatinized starch with 7 volumes of water) containing a coupling agent (amino-terminated silane coupling agent) and irradiated at a dose of 0.41 kGy for 13 minutes. C. Filter and remove the eggshell membrane powder, wash twice with water, and enzymatically hydrolyze with pepsin (pH 2, 37°C, 5 hours), then with alkaline protease (pH 9, 53°C, 1.5 hours), using an enzyme dosage of 7.5 U / mg. D. Ultrafiltration to obtain ultrafiltration separation components with a molecular weight of 5~10kDa.

[0046] 2. Weigh the raw materials: 170 g of 0.9% NaCl solution, 13 g of sodium hyaluronate, 2.3 g of lutein, 0.7 g of natural borneol powder (nanoscale), 22 g of cassia seed and wild chrysanthemum water extract (the mass ratio of cassia seed: wild chrysanthemum is 1.8:1), 21 g of eggshell membrane extract, 8 g of Rosa laevigata root triterpenoids (Rosa laevigata sapogenin, for preparation method, refer to 201810455214.6).

[0047] 3. Add sodium hyaluronate, lutein, natural borneol powder, cassia seed and wild chrysanthemum water extract, eggshell membrane extract, and triterpenoids from the root of Rosa laevigata to 0.9% NaCl solution, stir evenly at 2600 r / min, and sterilize to obtain an eye protection solution based on natural extracts.

[0048] How to use: You can pack the eye protection solution in a spray bottle and spray it into your eyes 2 to 3 times, 3 to 4 times a day.

[0049] Test Example 1 Experiment on the Effect of Examples 1-5 and Comparative Examples 1-2 on Tear Secretion in Dry Eye Model Rats Grouping: D1, 40 rats with qualified corneal fluorescence staining scores and tear secretion were selected and divided into 8 groups (model control group, experimental groups 1 to 5, and comparison groups 1 to 2), with 5 rats in each group and no significant statistical differences.

[0050] Modeling: On D2, 20 μL of hypertonic solution was instilled into the conjunctival sac of both eyes of the animal, 5 times / day, with an interval of about 2 hours between each instillation, for 27 consecutive days. After instillation, the animal's eyelids were passively closed for about 90 seconds to establish the model.

[0051] Administration: The model control group received no additional administration, and the experimental groups 1 to 5 were respectively administered with the eye protection solution prepared in Examples 1 to 5 (sprayed 24 times a day, approximately equivalent to 6 times the clinical dosage), and the comparative groups 1 to 2 were respectively administered with the eye protection solution prepared in Comparative Examples 1 to 2 (sprayed 24 times a day), and used continuously for 20 days.

[0052] Test: The tear secretion (mm2) of each group of rats was measured on D10 and D15, and the mean was calculated. The results are shown in Table 1.

[0053] Table 1 The tear secretion measurement results of each group of rats

[0054] As can be seen from Table 1, after 20 days of continuous administration, the tear secretion of rats basically returned to normal, and the drug can effectively treat dry eye in rats.

[0055] The eye protection liquid of the present invention is prepared from sodium hyaluronate, lutein, natural borneol powder, cassia seed and wild chrysanthemum flower water extract, eggshell membrane extract, and Rosa laevigata root triterpenoids. It has a small number of raw materials, contains multiple natural extracts, does not contain alcohol, has low irritation, is suitable for people with sensitive eyes, is simple to use, and can effectively improve dry eye syndrome.

[0056] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. An eye protection solution based on natural extracts, characterized in that: The method comprises the following raw materials in parts by weight: 100-200 parts of 0.9% NaCl solution, 12-14 parts of sodium hyaluronate, 1-3 parts of lutein, 0.1-1 parts of natural borneol powder, 16-25 parts of fermented extracts of cassia seeds and wild chrysanthemum, 17-22 parts of eggshell membrane extract, and 6-10 parts of triterpenes from the root of Rosa laevigata; The method for preparing the eggshell membrane extract comprises the following steps: A. Collect eggshell membranes, crush them, add 2-3 times the volume of 0.6% saline, and soak for 15-20 minutes; B. Take out the eggshell membrane powder and immerse it in acetic anhydride solution for 4-6 minutes; then immerse it in gelatinized starch solution containing coupling agent, and irradiate it at the same time, the irradiation dose is 0.35-0.42 kGy, and the irradiation time is 10-15 minutes; C. Filter out the eggshell membrane powder, wash it with water 1-3 times, first hydrolyze it with pepsin, then with alkaline protease, and add 7-8U / mg of enzyme; D. Ultrafiltration to obtain ultrafiltration separation components with a molecular weight of 5~10kDa.

2. The eye protection solution based on natural extracts according to claim 1, characterized in that: The eye protection solution is packaged in a spray bottle.

3. The eye protection solution based on natural extracts according to claim 2, characterized in that: The mass ratio of cassia seed to wild chrysanthemum in the fermentation and extract of cassia seed and wild chrysanthemum is 1.5-2:

1.

4. The eye protection solution based on natural extracts according to claim 3, characterized in that: The particle size of the natural borneol powder is nanometer level.

5. The eye protection solution based on natural extracts according to claim 4, characterized in that The concentration of the acetic anhydride solution is 1-1.3 mol / L.

6. The eye protection solution based on natural extracts according to claim 5, characterized in that: The coupling agent is an amino-terminated silane coupling agent.

7. The eye protection solution based on natural extracts according to claim 6, characterized in that: The gelatinized starch solution is prepared by mixing gelatinized starch with 4 to 8 times the volume of water.

8. The eye protection solution based on natural extracts according to claim 7, characterized in that: in, The preparation method of the fermentation and extract of Cassia seed and wild chrysanthemum is as follows: A. Wash Cassia seed and wild chrysanthemum, dry them in the sun, freeze them at a low temperature below -25°C and grind them into mixed plant powder; B. Add water and yeast to the plant mixed powder, mix well, ferment at 25-30℃ for 4-6h, then steam at 100-105℃ for 5-8min, take out and cool to 35-38℃ while stirring, then add lactic acid bacteria, mix well, ferment at 35-38℃ for 1-2h, then steam at 95-100℃ for 1-3min; C. Ultrasonic assisted extraction after centrifugation, with an ultrasonic power of 300-400 W, an ultrasonic frequency of 30-40 kHz, and a time of 25-30 min, followed by microfiltration, with a filter membrane pore size of 0.6-0.8 μm to obtain a filtrate; D. Adjust the pH of the filtrate to 7-7.2, extract with an equal volume of ethanol, recover the ethanol by vacuum distillation, and then filter with a filter membrane with a pore size of 0.5-0.7 μm to obtain a clear liquid, which is the fermentation and extract of Cassia seed and wild chrysanthemum.

9. The eye protection solution based on natural extracts according to claim 8, characterized in that: in, The triterpene compound of the root of Rosa laevigata is Rosa laevigata sapogenin.

10. A method for preparing an eye protection solution based on natural extracts according to claim 9, characterized in that: The steps are: add sodium hyaluronate, lutein, natural borneol powder, fermentation and extract of cassia seed and wild chrysanthemum, eggshell membrane extract, and triterpenoids of Rosa laevigata root into 0.9% NaCl solution, stir evenly at 2000-3000 r / min, and obtain eye protection solution based on natural extracts after sterilization.

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