Culture solution purifying and filtering device

Abstract

The invention belongs to the technical field of bioreactors, and particularly relates to a culture solution purifying and filtering device which comprises a tank body and a top cover clamped on the surface of the tank body, the top cover is provided with a long pipe and a short pipe, and the long pipe and the short pipe penetrate through the surface of the top cover and extend into the tank body. Clamping hoops which are symmetrically arranged are clamped to the edges of the tank body and the top cover, the two clamping hoops are hinged through a hinge buckle, and locking assemblies are arranged at the ends, away from the hinge buckle, of the two clamping hoops; the disposable filter container is adopted and serves as an important link in various purification device systems, the process that traditional equipment needs to enter a high-pressure container to be disinfected and the like is omitted, when a culture solution is purified and filtered, the device is easy and convenient to install and low in operation difficulty, and the device can be rapidly detached to be replaced after an experiment is completed; the condition of bacterial contamination of the cultured cells is avoided.

Description

Technical Field

[0001] The invention belongs to the technical field of bioreactors, and in particular relates to a culture fluid purification and filtering device. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro to enable cells to survive, grow, reproduce and maintain their main structures and functions. For some studies, cell culture is a commonly used technique, and a variety of different instruments are used when culturing cells.

[0003] After checking the publication (announcement) number: CN114874911A, a cell and excrement separation device is disclosed. This technology discloses "including a device body, the device body includes a reactor, a container, a peristaltic pump, a hollow fiber membrane column, a working tank, a delivery pipe, a glass tube, a sterile filtration device, a control valve, an air compressor and a vacuum pump, the reactor is connected to the hollow fiber membrane column through a pipeline, and the side of the hollow fiber membrane column is connected to one end of the peristaltic pump through a pipeline" and other technical solutions; it has "the control valve is connected to the air compressor and the vacuum pump respectively through the pipeline, the device omits the existing diaphragm pump and diaphragm for cell filtration, and adds a working tank, thereby preventing the cells from being damaged during filtration, and further more suitable for cell culture" and other technical effects;

[0004] However, the existing working tank needs to be sterilized in a high-pressure container to ensure the sterility of the tank body and avoid contamination. This working tank is inconvenient to clean and maintain, causing trouble to the experimenters.

[0005] In order to solve the above problems, the present application proposes a culture fluid purification and filtration device. Summary of the invention

[0006] In order to solve the problems raised in the above background technology, the present invention provides a culture fluid purification and filtering device, which has the characteristics of being easy to install and disassemble, disposable, and avoiding the cumbersome disinfection of the working tank.

[0007] To achieve the above-mentioned purpose, the present invention provides the following technical solution: a culture fluid purification and filtration device, comprising a tank body and a top cover engaged with the surface of the tank body, the top cover being provided with a long tube and a short tube, the long tube and the short tube passing through the surface of the top cover and extending into the tank body, symmetrically arranged clamps being engaged with the edges of the tank body and the top cover, two of the clamps being hingedly connected by a hinge buckle, and a locking assembly being provided at one end of the two clamps away from the hinge buckle.

[0008] As a preferred culture fluid purification and filtering device of the present invention, the side of the tank body that fits with the top cover is a concave boss structure, and the top cover matches the concave part of the edge of the tank body.

[0009] As a preferred culture fluid purification and filtration device of the present invention, the locking assembly includes a screw installed on one of the clamps, one end of the screw is rotatably connected to the clamp via a rotating shaft, and the other end of the screw is threadedly connected to a locking knob.

[0010] As a preferred culture fluid purification and filtration device of the present invention, a non-slip gasket is slidably connected to the surface of the screw, and the non-slip gasket is arranged on one side of the locking knob and fits with the locking knob.

[0011] As a preferred culture fluid purification and filtering device of the present invention, a groove is provided on the inner side of the clamp, and a silicone gasket is installed at the groove.

[0012] Compared with the prior art, the present invention has the following beneficial effects:

[0013] The present invention adopts a disposable filter container as an important part of various purification device systems, eliminating the need for traditional equipment to be sterilized in a high-pressure container. When purifying and filtering the culture medium, the present invention is simple and convenient to install and has low operating difficulty. After the experiment is completed, it can be quickly disassembled and replaced to avoid bacterial contamination of the cultured cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0015] Figure 1 It is a structural schematic diagram of the present invention;

[0016] Figure 2 It is a schematic diagram of the structure of the clamp in the present invention;

[0017] Figure 3 It is a structural schematic diagram of the tank body in the present invention;

[0018] Figure 4 It is a schematic diagram of the structure of the top cover in the present invention;

[0019] In the figure: 1. tank body; 2. top cover; 3. long tube; 4. short tube; 5. clamp; 51. slot; 6. hinge buckle; 7. screw; 8. locking knob; 9. anti-slip gasket; 10. locking assembly. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] Example 1

[0022] After checking the publication (announcement) number: CN114874911A, a cell and excrement separation device is disclosed. This technology discloses "including a device body, the device body includes a reactor, a container, a peristaltic pump, a hollow fiber membrane column, a working tank, a delivery pipe, a glass tube, a sterile filtration device, a control valve, an air compressor and a vacuum pump, the reactor is connected to the hollow fiber membrane column through a pipeline, and the side of the hollow fiber membrane column is connected to one end of the peristaltic pump through a pipeline" and other technical solutions; it has "the control valve is connected to the air compressor and the vacuum pump respectively through the pipeline, the device omits the existing diaphragm pump and diaphragm for cell filtration, and adds a working tank, thereby preventing the cells from being damaged during filtration, and further more suitable for cell culture" and other technical effects;

[0023] However, the existing working tank needs to be sterilized in a high-pressure container to ensure the sterility of the tank body and avoid contamination. This working tank is inconvenient to clean and maintain, which causes trouble to the experimenters. The filtration operation of the culture medium in the present invention is the same as that in the background technology and will not be described here. In order to solve the above problems, the following improvement plan is proposed.

[0024] More specifically:

[0025] like Figure 1-Figure 4 As shown;

[0026] Combining the above:

[0027] The culture fluid purification and filtering device comprises a tank body 1 and a top cover 2 engaged on the surface of the tank body 1, a long tube 3 and a short tube 4 are arranged on the top cover 2, the long tube 3 and the short tube 4 penetrate the surface of the top cover 2 and extend into the tank body 1, symmetrically arranged clamps 5 are engaged at the edges of the tank body 1 and the top cover 2, the two clamps 5 are hingedly connected by a hinge buckle 6, and a locking assembly 10 is arranged at one end of the two clamps 5 away from the hinge buckle 6.

[0028] In this embodiment: when the user uses the present invention, the external air source is connected to the short tube 4 through a hose, the long tube 3 is connected to the filtering device, and then the top cover 2 is installed in the tank body 1, and the purification operation can be carried out. The air in the tank body 1 is extracted through the air source, so that the tank body 1 forms a negative pressure, so that the culture fluid can enter the tank body 1. Since the culture fluid entering the tank body 1 is filtered, the extraction and impurity removal of the culture fluid are completed. When the purified culture fluid needs to be used, the hose at the long tube 3 is removed, and pure air is injected into the tank body 1 through the external air source, so that the tank body 1 can discharge the culture fluid from the long tube 3, which is convenient for users to use.

[0029] It should be noted that the present invention is a disposable container, the main material of which is a polymer material such as polyethylene.

[0030] It should be noted that the exposed ends of the long tube 3 and the short tube 4 are both pagoda structures.

[0031] Going further:

[0032] In an optional embodiment, the side of the tank body 1 that fits the top cover 2 is a concave boss structure, and the top cover 2 matches the concave portion of the edge of the tank body 1 .

[0033] In this embodiment: through this design, after the tank body 1 and the top cover 2 are combined, the entire surface is in the same plane, which is convenient for installing the clamp 5 and improves the air tightness of the present invention to prevent air from entering the tank body 1.

[0034] Going further:

[0035] In an optional embodiment, the locking assembly 10 includes a screw rod 7 installed on one of the clamps 5 , one end of the screw rod 7 is rotatably connected to the clamp 5 via a rotating shaft, and the other end of the screw rod 7 is threadedly connected to a locking knob 8 .

[0036] In this embodiment: through this design, the tank body 1 and the top cover 2 are disposable. When the user needs to replace the tank body 1 and the top cover 2, the user can turn the locking knob 8 to separate the locking knob 8 from the clamp 5. At this time, the user can turn the screw 7 to screw the screw 7 out from one side of the clamp 5. The two clamps 5 are hinged by the hinge buckle 6 and can be rotated. The user can open the clamp 5 and replace the new tank body 1. The above operation can be reversed during installation. The operation is simple and convenient to use.

[0037] Going further:

[0038] In an optional embodiment, a non-slip gasket 9 is slidably connected to the surface of the screw rod 7 , and the non-slip gasket 9 is arranged on one side of the locking knob 8 and fits with the locking knob 8 .

[0039] In this embodiment: through this design, the anti-slip gasket 9 makes the locking knob 8 and the clamp 5 fit tightly together, which is stable and firm, and prevents the screw rod 7 from falling off during use.

[0040] Going further:

[0041] In an optional embodiment, a clamping groove 51 is formed on the inner side of the clamp 5 , and a silicone gasket is installed in the clamping groove 51 .

[0042] In this embodiment: through this design, the clamp 5 can be clamped to the top of the tank body 1 and the top cover 2, thereby improving the connection strength between the clamp 5 and the tank body 1 and avoiding the separation of the clamp 5 and the tank body 1 during use. The silicone gasket can further improve the air tightness of the present invention.

[0043] The working principle and use process of the present invention are as follows: the tank body 1 and the top cover 2 are disposable. When the user needs to replace the tank body 1 and the top cover 2, the user can rotate the locking knob 8 to separate the locking knob 8 from the clamp 5. At this time, the user can rotate the screw rod 7 to screw the screw rod 7 out from one side of the clamp 5. The two clamps 5 are hinged by the hinge buckle 6 and can be rotated. The user can open the clamp 5 and replace the new tank body 1. When installing, the above operation can be reversed. The operation is simple and convenient to use. When the user uses the present invention, the external air source is connected through a hose. On the short tube 4, the long tube 3 is connected to the filtering equipment, and then the top cover 2 is installed in the tank body 1 to carry out the purification operation. The air in the tank body 1 is extracted through the air source to form a negative pressure in the tank body 1, so that the culture fluid can enter the tank body 1. Since the culture fluid entering the tank body 1 is filtered, the extraction and impurity removal of the culture fluid are completed. When the purified culture fluid needs to be used, the hose at the long tube 3 is removed, and pure air is injected into the tank body 1 through an external air source, so that the tank body 1 can discharge the culture fluid from the long tube 3, which is convenient for users to use.

[0044] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A culture fluid purification and filtration device, characterized in that: The invention comprises a tank body (1) and a top cover (2) engaged with the surface of the tank body (1); a long tube (3) and a short tube (4) are provided on the top cover (2); the long tube (3) and the short tube (4) penetrate the surface of the top cover (2) and extend into the tank body (1); symmetrically arranged clamps (5) are engaged at the edges of the tank body (1) and the top cover (2); two clamps (5) are hingedly connected by a hinge buckle (6); and a locking assembly (10) is provided at one end of the two clamps (5) away from the hinge buckle (6).

2. The culture fluid purification and filtration device according to claim 1, characterized in that: The side of the tank body (1) that fits the top cover (2) is a concave boss structure, and the top cover (2) matches the concave portion of the edge of the tank body (1).

3. The culture fluid purification and filtration device according to claim 1, characterized in that: The locking assembly (10) comprises a screw rod (7) mounted on one of the clamps (5), one end of the screw rod (7) being rotatably connected to the clamp (5) via a rotating shaft, and the other end of the screw rod (7) being threadedly connected to a locking knob (8).

4. The culture fluid purification and filtration device according to claim 3, characterized in that: The surface of the screw rod (7) is slidably connected with an anti-skid pad (9), and the anti-skid pad (9) is arranged on one side of the locking knob (8) and fits with the locking knob (8).

5. The culture fluid purification and filtration device according to claim 1, characterized in that: A clamping groove (51) is provided on the inner side of the clamp (5), and a silicone gasket is installed at the clamping groove (51).

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