Small-size totally-enclosed microscope capable of observing in range

Abstract

The invention relates to the technical field of microscopes, and discloses a small-size totally-enclosed microscope capable of observing in a range, which comprises a sealed shell, six supporting columns are mounted in the sealed shell through bolts, and a mounting bottom plate is mounted at the bottoms of the six supporting columns through bolts. An X-axis moving structure is fixedly mounted at the top of the mounting bottom plate, and a fixing groove is formed in one side of the top of the mounting bottom plate; the X-axis moving structure comprises a side mounting plate and a supporting plate; by arranging the X-axis moving structure and the Y-axis moving structure, when the living cell culture container is observed, the living cell culture container is clamped in the placing groove, and the X-axis moving structure and the Y-axis moving structure are controlled to be matched with each other, so that the living cell culture container can be tracked and observed; therefore, the accuracy of microscopic observation data is improved.

Description

Technical Field

[0001] The invention relates to the technical field of microscopes, in particular to a fully enclosed microscope with a small volume and capable of range observation. Background Art

[0002] Currently, in the field of biological cell culture, there is a huge demand for tracking observation of living cells during culture. It has important background significance for deepening the understanding of life processes, disease mechanisms, drug screening and gene therapy, and has a positive impact on promoting the development of medicine and biology.

[0003] An effective way to observe living cells is to use an inverted microscope. However, the inverted microscopes currently on the market are relatively large in size. When performing small-scale synchronous observations such as culturing living cells or drug screening, they cannot be placed in a cell culture incubator due to their large size. At the same time, existing devices are not easy to meet the needs of long-term tracking and observation of cells during living cell culture. Based on this, the present invention applies to design a small-volume, fully enclosed microscope that can be used for range observation. Summary of the invention

[0004] In view of the deficiencies of the prior art, the present invention provides a fully enclosed microscope with a small volume that can observe a wide range, which solves the problems mentioned in the above background that the existing inverted microscope cannot be placed in a cell culture box and cannot meet the requirements of long-term tracking and observation of living cells.

[0005] The present invention provides the following technical solution: a fully enclosed microscope with a small volume and capable of range observation, comprising: a sealed shell, six supporting columns are installed inside the sealed shell by bolts, a mounting base plate is installed at the bottom of the six supporting columns by bolts, an X-axis moving structure is fixedly installed on the top of the mounting base plate, and a fixing groove is provided on one side of the top of the mounting base plate; the X-axis moving structure comprises a side mounting plate and a support plate, an X-axis motor is fixedly installed on one side of the outer surface of the side mounting plate, the X-axis motor is placed in the fixing groove, a first pulley is fixedly installed on one end of the output shaft of the X-axis motor, a second pulley is rotatably installed on one side of the outer surface of the side mounting plate, a first linkage belt is sleeved between the first pulley and the second pulley, and an X-axis wire is rotatably installed on one side of the support plate Rod, one end of the X-axis screw rod is fixedly connected to the second pulley, the outer surface of the X-axis screw rod is threadedly installed with a first movable plate, one side of the first movable plate is slidably engaged with the mounting base plate, and the top of the first movable plate is fixedly installed with a Y-axis movable structure; the Y-axis movable structure includes an L-shaped plate, the L-shaped plate is fixedly connected to the first movable plate, one side of the outer surface of the L-shaped plate is fixedly installed with a Y-axis motor, one end of the output shaft of the Y-axis motor is fixedly installed with a third pulley, and one side of the outer surface of the L-shaped plate is rotatably installed with a fourth pulley, a second linkage belt is sleeved between the fourth pulley and the third pulley, one end of the fourth pulley is fixedly installed with a Y-axis screw rod, and the outer surface of the Y-axis screw rod is threadedly installed with a second movable plate, and the second movable plate is slidably engaged with the first movable plate.

[0006] Preferably, a Z-axis moving structure is fixedly mounted on one side of the outer surface of the second moving plate, and the Z-axis moving structure includes a mounting seat, and the mounting seat is fixedly connected to the second moving plate.

[0007] Preferably, a Z-axis motor is fixedly mounted on one side of the bottom of the mounting seat, a fifth pulley is fixedly mounted on one end of the output shaft of the Z-axis motor, and a sixth pulley is rotatably mounted on one side of the top of the mounting seat.

[0008] Preferably, a third linkage belt is sleeved between the sixth pulley and the fifth pulley, a Z-axis screw is fixedly installed on one side of the sixth pulley, a connecting block is threadedly installed on the outer surface of the Z-axis screw, and the connecting block is slidably connected to the mounting seat.

[0009] Preferably, a microscope system is fixedly mounted on one side of the top of the second movable plate, an objective lens for observation is provided on one side of the microscope system, and the objective lens is fixedly connected to the connecting block.

[0010] Preferably, a C-shaped rigid connecting plate is installed on one side of the outer surface of the second movable plate by bolts, and a light source is fixedly installed on one side of the bottom of the C-shaped rigid connecting plate, and the light source coincides with the center point of the objective lens.

[0011] Preferably, a carrying platform is provided on the top of the sealed housing, and a placement groove is provided inside the carrying platform.

[0012] Preferably, a vertical slot plate is fixedly mounted on one side of the top of the mounting platform, and a slot is formed inside the vertical slot plate and is communicated with the inside of the sealed housing.

[0013] Preferably, a transverse groove plate is fixedly mounted on one side of the outer surface of the vertical groove plate, and a groove is formed inside the transverse groove plate and is communicated with the inner groove of the vertical groove plate.

[0014] Preferably, a light source protection frame is fixedly mounted on the bottom of the transverse groove plate, and a glass window is fixedly mounted on the bottom of the light source protection frame.

[0015] Compared with the prior art, the present invention has the following beneficial effects: 1. By setting the X-axis moving structure and the Y-axis moving structure, when observing the living cell culture container, by clamping the living cell culture container in the placement groove, the second moving plate and the C-shaped rigid connecting plate are rigidly connected, so that the light source and the objective lens when the microscope system moves can always be kept in a vertical state, and the first pulley can be driven by the first linkage belt to drive the second pulley to rotate through the drive of the X-axis motor, so that the X-axis lead screw can rotate on the support plate, and then the first moving plate can be horizontally moved on the installation base plate, and at the same time, the third pulley can be driven by the second linkage belt to rotate through the drive of the Y-axis motor. The fourth pulley is driven to rotate, thereby rotating the Y-axis screw rod. Since the second movable plate is slidably connected to the first movable plate, the rotation of the Y-axis screw rod can drive the second movable plate to move vertically and horizontally. By controlling the two to cooperate with each other, the living cell culture container can be tracked and observed, thereby improving the accuracy of microscopic observation data. At the same time, due to the setting of the sealed shell and the microscopic system, the small volume design of the sealed shell can meet the small-range observation of the internal device. At the same time, when the device is placed in the cell culture box, the active cells are tracked and observed through the objective lens, and the image is transmitted to the outside through the microscopic system for observation; 2. Through the Z-axis moving structure, when observing the living cell culture container, the fifth pulley drives the sixth pulley to rotate through the third linkage belt through the driving of the mounting seat, so that the sixth pulley can drive the Z-axis screw rod to rotate, so that the connecting block can drive the objective lens in the microscope system to move up and down, thereby adjusting the height of the living cell culture container for observation, so as to observe the living cell culture container more clearly; 3. By setting up a sealed shell, when the living cell culture container is placed in the placement groove, the sealed shell can ensure that the interior is completely sealed, so that the device and optical elements in the sealed shell are not polluted by water vapor and dust, thereby improving the service life of the device and ensuring the observation accuracy of the optical elements. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the appearance structure of a fully enclosed microscope with a small volume and capable of range observation according to the present invention; Figure 2 This is a schematic diagram of the bottom plate structure of a fully enclosed microscope with a small volume and capable of range observation according to the present invention; Figure 3 This is a schematic diagram of the structure of a fully enclosed microscope glass window with a small volume and capable of range observation according to the present invention; Figure 4 This is a schematic diagram of the structure of a C-shaped rigid connecting plate of a fully enclosed microscope with a small volume and capable of range observation according to the present invention; Figure 5 This is a schematic diagram of the X-axis movement structure of a fully enclosed microscope with a small volume and capable of range observation according to the present invention; Figure 6 This is a schematic diagram of the structure of a fully enclosed microscope support plate with a small volume and capable of range observation according to the present invention; Figure 7 This is a schematic diagram of the Y-axis moving structure of a fully enclosed microscope with a small volume and capable of range observation according to the present invention; Figure 8 The present invention is a schematic diagram of the Z-axis moving structure of a fully enclosed microscope with a small volume and capable of range observation.

[0017] In the figure: 1, sealed housing; 2, loading platform; 3, placement slot; 4, vertical slot plate; 5, horizontal slot plate; 6, support column; 7, installation base plate; 8, light source frame; 9, glass window; 10, X-axis moving structure; 101, side mounting plate; 102, X-axis motor; 103, first pulley; 104, first linkage belt; 105, second pulley; 106, support plate; 107, X-axis screw rod; 11, first moving plate; 12, Y-axis moving structure; 121, L-shaped plate; 122, Y-axis motor; 123, third pulley; 124, second linkage belt; 125, fourth pulley; 126, Y-axis lead screw; 127, second movable plate; 13, Z-axis movable structure; 131, mounting seat; 132, Z-axis motor; 133, fifth pulley; 134, third linkage belt; 135, sixth pulley; 136, Z-axis lead screw; 137, connecting block; 14, microscope system; 15, C-shaped rigid connecting plate; 16, light source. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] See also Figure 1-8 A fully enclosed microscope with a small volume and capable of range observation comprises: a sealed shell 1, six supporting columns 6 are installed inside the sealed shell 1 by bolts, a mounting base plate 7 is installed at the bottom of the six supporting columns 6 by bolts, an X-axis moving structure 10 is fixedly installed on the top of the mounting base plate 7, and a fixing groove is provided on one side of the top of the mounting base plate 7; the X-axis moving structure 10 comprises a side mounting plate 101 and a supporting plate 106, an X-axis motor 102 is fixedly installed on one side of the outer surface of the side mounting plate 101, the X-axis motor 102 is placed in the fixing groove, a first pulley 103 is fixedly installed on one end of the output shaft of the X-axis motor 102, a second pulley 105 is rotatably installed on one side of the outer surface of the side mounting plate 101, a first linkage belt 104 is sleeved between the first pulley 103 and the second pulley 105, an X-axis screw rod 107 is rotatably installed on one side of the supporting plate 106, and one end of the X-axis screw rod 107 is connected to the The second pulley 105 is fixedly connected, and the first movable plate 11 is threadedly installed on the outer surface of the X-axis screw rod 107, and one side of the first movable plate 11 is slidably engaged with the mounting base plate 7, and a Y-axis movable structure 12 is fixedly installed on the top of the first movable plate 11; the Y-axis movable structure 12 includes an L-shaped plate 121, and the L-shaped plate 121 is fixedly connected to the first movable plate 11, and a Y-axis motor 122 is fixedly installed on one side of the outer surface of the L-shaped plate 121, and a third pulley 123 is fixedly installed on one end of the output shaft of the Y-axis motor 122, and a fourth pulley 125 is rotatably installed on one side of the outer surface of the L-shaped plate 121, and a second linkage belt 124 is sleeved between the fourth pulley 125 and the third pulley 123, and a Y-axis screw rod 126 is fixedly installed on one end of the fourth pulley 125, and a second movable plate 127 is threadedly installed on the outer surface of the Y-axis screw rod 126, and the second movable plate 127 is slidably engaged with the first movable plate 11.

[0020] See also Figure 4 , Figure 6 and Figure 7 Figure 8The Z-axis moving structure 13 is fixedly installed on one side of the outer surface of the second moving plate 127. The Z-axis moving structure 13 includes a mounting seat 131, and the mounting seat 131 is fixedly connected to the second moving plate 127. A Z-axis motor 132 is fixedly installed on one side of the bottom of the mounting seat 131, and a fifth pulley 133 is fixedly installed on one end of the output shaft of the Z-axis motor 132. A sixth pulley 135 is rotatably installed on one side of the top of the mounting seat 131. A third linkage belt 134 is sleeved between the sixth pulley 135 and the fifth pulley 133, and a Z-axis screw rod 136 is fixedly installed on one side of the sixth pulley 135. A connecting block 137 is threadedly installed on the outer surface of the Z-axis screw rod 136, and the connecting block 137 is slidably connected to the mounting seat 131. A microscope system 14 is fixedly installed on one side of the top of the second moving plate 127, and an objective lens for observation is provided on one side of the microscope system 14, and the objective lens is fixedly connected to the connecting block 137. A C-shaped rigid connecting plate 15 is installed on one side of the outer surface of the second movable plate 127 by bolts, and a light source 16 is fixedly installed on one side of the bottom of the C-shaped rigid connecting plate 15, and the light source 16 coincides with the center point of the objective lens.

[0021] The fifth pulley 133 drives the sixth pulley 135 to rotate through the third linkage belt 134 through the drive of the mounting seat 131, thereby the sixth pulley 135 can drive the Z-axis screw rod 136 to rotate, so that the connecting block 137 can drive the objective lens in the microscope system 14 to move up and down, thereby adjusting the height of the living cell culture container for observation. Since the C-shaped rigid connecting plate 15 is connected to the second movable plate 127, and the light source 16 coincides with the center point of the objective lens on the microscope system 14, when the second movable plate 127 moves, the light source 16 and the objective lens always remain consistent.

[0022] See also Figure 1-3 A carrying platform 2 is provided on the top of the sealed housing 1, and a placement groove 3 is provided inside the carrying platform 2. A vertical groove plate 4 is fixedly installed on one side of the top of the carrying platform 2, and a groove is provided inside the vertical groove plate 4 and communicates with the inside of the sealed housing 1. A horizontal groove plate 5 is fixedly installed on one side of the outer surface of the vertical groove plate 4, and a groove is provided inside the horizontal groove plate 5 and communicates with the groove inside the vertical groove plate 4. A light source guard frame 8 is fixedly installed on the bottom of the horizontal groove plate 5, and a glass window 9 is fixedly installed on the bottom of the light source guard frame 8.

[0023] When the living cell culture container is placed in the placement groove 3, the sealed housing 1 can ensure that the interior thereof is completely sealed, so that the device and optical elements in the sealed housing 1 are not polluted by water vapor and dust. By setting the glass window 9, the light source 16 can be not blocked when following the movement of the objective lens.

[0024] Working principle, first, by clamping the living cell culture container in the placement groove 3, the sealed shell 1 can ensure that its interior is completely sealed, so that the device and optical elements in the sealed shell 1 are not polluted by water vapor and dust, and then the second movable plate 127 is rigidly connected with the C-shaped rigid connecting plate 15, so that the light source 16 and the objective lens when the microscope system 14 moves can always maintain a vertical state, and through the drive of the X-axis motor 102, the first pulley 103 can drive the second pulley 105 to rotate through the first linkage belt 104, so that the X-axis lead screw 107 can rotate on the support plate 106, and then the first movable plate 11 can be horizontally moved horizontally on the mounting base plate 7, and at the same time, through the drive of the Y-axis motor 122, the third pulley 123 can be driven by the second linkage belt The belt 124 drives the fourth pulley 125 to rotate, thereby the Y-axis screw rod 126 can be rotated. Since the second movable plate 127 is slidably connected to the first movable plate 11, the rotation of the Y-axis screw rod 126 can drive the second movable plate 127 to move vertically and horizontally. By controlling the two to cooperate with each other, the living cell culture container can be tracked and observed. Then, through the driving of the mounting seat 131, the fifth pulley 133 drives the sixth pulley 135 to rotate through the third linkage belt 134, thereby the sixth pulley 135 drives the Z-axis screw rod 136 to rotate, so that the connecting block 137 can drive the objective lens in the microscope system 14 to move up and down, thereby adjusting the height of the living cell culture container for observation, so as to observe the living cell culture container more clearly.

[0025] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A fully enclosed microscope with a small volume and capable of range observation, characterized in that: include: A sealed housing (1), wherein six supporting columns (6) are installed inside the sealed housing (1) by means of bolts, a mounting base plate (7) is installed at the bottom of the six supporting columns (6) by means of bolts, an X-axis moving structure (10) is fixedly installed at the top of the mounting base plate (7), and a fixing groove is provided on one side of the top of the mounting base plate (7); The X-axis moving structure (10) comprises a side mounting plate (101) and a support plate (106); an X-axis motor (102) is fixedly mounted on one side of an outer surface of the side mounting plate (101); the X-axis motor (102) is placed in a fixed groove; a first pulley (103) is fixedly mounted on one end of an output shaft of the X-axis motor (102); a second pulley (105) is rotatably mounted on one side of an outer surface of the side mounting plate (101); the first pulley (103) and the second pulley (105) are rotatably mounted on one side of the outer surface of the side mounting plate (101); 105), a first linkage belt (104) is sleeved between the support plate (106), an X-axis screw rod (107) is rotatably mounted on one side of the support plate (106), one end of the X-axis screw rod (107) is fixedly connected to the second pulley (105), a first movable plate (11) is threadedly mounted on the outer surface of the X-axis screw rod (107), one side of the first movable plate (11) is slidably engaged with the mounting base plate (7), and a Y-axis movable structure (12) is fixedly mounted on the top of the first movable plate (11); The Y-axis moving structure (12) comprises an L-shaped plate (121), the L-shaped plate (121) being fixedly connected to the first moving plate (11), a Y-axis motor (122) being fixedly mounted on one side of the outer surface of the L-shaped plate (121), a third pulley (123) being fixedly mounted on one end of the output shaft of the Y-axis motor (122), a fourth pulley (125) being rotatably mounted on one side of the outer surface of the L-shaped plate (121), a second linkage belt (124) being sleeved between the fourth pulley (125) and the third pulley (123), a Y-axis screw rod (126) being fixedly mounted on one end of the fourth pulley (125), a second moving plate (127) being threadedly mounted on the outer surface of the Y-axis screw rod (126), and the second moving plate (127) being slidably engaged with the first moving plate (11).

2. A small-volume, fully enclosed microscope capable of range observation according to claim 1, characterized in that: A Z-axis moving structure (13) is fixedly mounted on one side of the outer surface of the second moving plate (127); the Z-axis moving structure (13) comprises a mounting seat (131); and the mounting seat (131) is fixedly connected to the second moving plate (127).

3. A small-volume, fully enclosed microscope capable of range observation according to claim 2, characterized in that: A Z-axis motor (132) is fixedly mounted on one side of the bottom of the mounting seat (131), a fifth pulley (133) is fixedly mounted on one end of the output shaft of the Z-axis motor (132), and a sixth pulley (135) is rotatably mounted on one side of the top of the mounting seat (131).

4. A small-volume, fully enclosed microscope capable of range observation according to claim 3, characterized in that: A third linkage belt (134) is sleeved between the sixth pulley (135) and the fifth pulley (133); a Z-axis screw rod (136) is fixedly mounted on one side of the sixth pulley (135); a connecting block (137) is threadedly mounted on the outer surface of the Z-axis screw rod (136); and the connecting block (137) is slidably connected to the mounting seat (131).

5. A small-volume fully enclosed microscope capable of range observation according to claim 1, characterized in that: A microscope system (14) is fixedly mounted on one side of the top of the second movable plate (127), and an objective lens for observation is provided on one side of the microscope system (14), and the objective lens is fixedly connected to the connecting block (137).

6. A small-volume, fully enclosed microscope capable of range observation according to claim 5, characterized in that: A C-shaped rigid connection plate (15) is mounted on one side of the outer surface of the second movable plate (127) via bolts, and a light source (16) is fixedly mounted on one side of the bottom of the C-shaped rigid connection plate (15), wherein the light source (16) coincides with the center point of the objective lens.

7. A small-volume, fully enclosed microscope capable of range observation according to claim 1, characterized in that: A carrying platform (2) is provided on the top of the sealed housing (1), and a placement groove (3) is provided inside the carrying platform (2).

8. A small-volume, fully enclosed microscope capable of range observation according to claim 7, characterized in that: A vertical slot plate (4) is fixedly mounted on one side of the top of the carrying platform (2); a slot is provided inside the vertical slot plate (4) and is in communication with the inside of the sealed housing (1).

9. A small-volume, fully enclosed microscope capable of range observation according to claim 8, characterized in that: A transverse groove plate (5) is fixedly mounted on one side of the outer surface of the vertical groove plate (4); a groove is provided inside the transverse groove plate (5) and is communicated with the inner groove of the vertical groove plate (4).

10. A small-volume, fully enclosed microscope capable of range observation according to claim 9, characterized in that: A light source protection frame (8) is fixedly mounted on the bottom of the transverse groove plate (5), and a glass window (9) is fixedly mounted on the bottom of the light source protection frame (8).

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