Propagation method of small-leaf red beans

Abstract

The invention provides a lobular red bean propagation method which comprises the following steps: 1) soaking a lobular red bean seed for 2-4 hours by utilizing a probiotic aqueous solution to obtain a soaked seed; 2) performing heating treatment on the soaked seeds at 40-60 DEG C for 2-4 hours to obtain activated seeds; and (3) sequentially performing germination culture, strong seedling culture and seedling hardening culture on the activated seeds to obtain the jumble bead rooting seedlings. According to the propagation method of the ormosia microphylla, provided by the invention, the ormosia microphylla seeds are subjected to soaking treatment through the probiotic aqueous solution for sterilization, and the soaked seeds are subjected to heating treatment, so that dormancy of the ormosia microphylla seeds is broken, germination is promoted, and the purposes of fast seedling emergence and high germination rate can be achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a propagation method of small-leaf red beans. Background Art

[0002] In the "China Biodiversity Red List - Higher Plants Volume" jointly compiled by the Ministry of Environmental Protection and the Chinese Academy of Sciences in 2013, the small-leaved red bean was only classified as NT grade. In the newly revised "National Key Protected Wild Plant List" in September 2021, the small-leaved red bean was upgraded to a national first-class protected plant. The small-leaved red bean is a deciduous tree of the genus Red Bean in the Leguminosae family. Also known as Guangxi red sandalwood, it is a multi-value tree species that integrates timber, medicine, and landscape. It has strict requirements on the living environment, poor seed coat permeability, low seed setting rate, and serious diseases and insect pests. The small-leaved red bean shows a declining type and cannot regenerate naturally under the forest. With the progress and succession of the community, it will be replaced by other species. Due to long-term over-logging, the current stock of small-leaved red beans is scarce and it is an endangered species.

[0003] At present, there are few mother trees of small-leafed red beans, and the seed coat is hard, waxy, and shiny, which is not easy to absorb water and germinate. Generally, artificial seed sowing is adopted for cultivation, which takes 2 years or more from sowing to germination, and the germination rate is low. Therefore, developing a breeding method for small-leafed red beans with fast germination and high germination rate has become a technical problem that needs to be solved urgently at this stage. Summary of the invention

[0004] The main purpose of the invention is to provide a propagation method of small-leaf red beans, which has fast seedling emergence and high germination rate.

[0005] The present invention provides a method for propagating small-leaf red beans, comprising the following steps:

[0006] 1) soaking red bean seeds in a probiotic aqueous solution for 2-4 hours to obtain soaked seeds;

[0007] 2) heating the soaked seeds at 40-60° C. for 2-4 hours to obtain activated seeds;

[0008] 3) The activated seeds are subjected to germination culture, seedling hardening culture and seedling hardening culture in sequence to obtain rooted seedlings of small-leaf red beans.

[0009] In the breeding method as described above, the probiotics include at least one of Bacillus subtilis, Bacillus licheniformis and Bacillus megaterium.

[0010] In the breeding method as described above, the concentration of the probiotic aqueous solution is 40-50%.

[0011] In the propagation method as described above, the pH value of the substrate for hardening the seedlings is 5.5-6.0.

[0012] In the above-mentioned propagation method, the substrate for hardening and culturing is prepared by a method comprising the following steps:

[0013] The substrate is mixed with a sulfur powder solution with a mass concentration of 10-20%, heated at 121° C. for 20-50 minutes, cooled, and air-dried to a water content of 20-30% to obtain the substrate for seedling hardening culture.

[0014] In the propagation method as described above, the volume ratio of the substrate to the sulfur powder solution is 2-3:1-2.

[0015] The propagation method as described above, wherein the substrate comprises: peat, perlite, wood chips, and fine sand in a volume ratio of 2-3:1:1:1-3; and / or,

[0016] The culture solution used for the strong seedling culture comprises: 0.1-0.5 mg / L 6-BA and 0.1-0.5 mg / L NAA.

[0017] In the propagation method as described above, the matrix comprises: peat, perlite, wood chips and fine sand in a volume ratio of 2-3:1:1:1-3.

[0018] In the propagation method as described above, the culture solution used for culturing strong seedlings includes: 0.3 mg / L 6-BA, 0.3 mg / L NAA.

[0019] The propagation method as described above, wherein the germination culture comprises: culturing the activated seeds in a dark culture room at 18-25° C. for 1-2 days, and then transferring them to a light culture room at 18-25° C. and a light intensity of 1800-2500 lx to obtain 18-25 cm small-leaf red bean seedlings; and / or,

[0020] The temperature of the seedling cultivation is 18-25°C and the light intensity is 1800-2500lx; and / or,

[0021] The temperature of the seedling hardening culture is 22-30° C., and the light intensity is 2500-3000 lx.

[0022] The method for propagating small-leaf red beans provided by the present invention comprises the following steps: soaking small-leaf red bean seeds in a probiotic aqueous solution to sterilize them, and heating the soaked seeds to break the dormancy of the small-leaf red bean seeds and promote germination, thereby achieving the goals of fast seedling emergence and high germination rate. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical solutions and advantages of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0024] The present invention provides a method for propagating small-leaf red beans, comprising the following steps:

[0025] 1) soaking red bean seeds in a probiotic aqueous solution for 2-4 hours to obtain soaked seeds;

[0026] 2) heating the soaked seeds at 40-60° C. for 2-4 hours to obtain activated seeds;

[0027] 3) The activated seeds are subjected to germination culture, seedling hardening culture and seedling hardening culture in sequence to obtain rooted seedlings of small-leaf red beans.

[0028] The invention soaks the small-leaf red bean seeds in a probiotic aqueous solution, and then heats the soaked seeds, so that the finally obtained small-leaf red bean rooted seedlings can have fast emergence and high germination rate.

[0029] Specifically, in step 1), the small-leaf red bean seeds in the present invention can be small-leaf red bean seeds stored in a 4°C seed storage cabinet for 5 years. After taking out, the seeds are placed in a bucket filled with water, and the unripe seeds and empty grains floating on the water surface are removed. The seeds with complete appearance, fullness, uniform grains, no mildew and pests are selected from the seeds that sink to the bottom. Then the selected small-leaf red bean seeds are poured into a certain concentration of probiotic aqueous solution and mixed, and soaked for 2-4h. The ambient temperature can be 4-10°C to obtain the soaked small-leaf red bean seeds. The beneficial microorganisms in the probiotic aqueous solution can be used to inhibit and reduce the number of harmful microorganisms on the surface and inside of the seeds, such as bacteria, fungi, etc., thereby reducing the risk of seed infection and disease, so as to achieve sterilization of the small-leaf red bean seeds and improve the germination rate of the seeds.

[0030] In step 2), the soaked small-leaf red bean seeds obtained in step 1) are washed and then heated in an oven at 40-60° C. for 2-4 hours to break the dormancy of the small-leaf red bean seeds, promote seed germination, and obtain activated seeds.

[0031] In step 3), the activated seeds obtained above are subjected to germination culture and seedling culture in sequence to obtain seedlings, and then to seedling hardening culture, and finally rooted seedlings of small-leaf red beans can be obtained.

[0032] The present invention does not limit the culture conditions of germination culture, seedling hardening culture and seedling hardening culture, as long as the rooted seedlings of small-leaf red beans can be finally obtained.

[0033] The method for propagating small-leaf red beans provided by the present invention comprises the following steps: soaking small-leaf red bean seeds in a probiotic aqueous solution to sterilize them, and heating the soaked seeds to break the dormancy of the small-leaf red bean seeds and promote germination, thereby achieving the goals of fast seedling emergence and high germination rate.

[0034] In some embodiments of the present invention, the probiotics include at least one of Bacillus subtilis, Bacillus licheniformis, and Bacillus megaterium.

[0035] The probiotics of the present invention include at least one of Bacillus subtilis, Bacillus licheniformis and Bacillus megaterium, which can further improve the bactericidal effect on small-leaf red bean seeds, thereby achieving the goals of fast emergence and high germination rate.

[0036] In some embodiments of the present invention, the concentration of the probiotic aqueous solution is 40-50%.

[0037] The concentration of the probiotic aqueous solution in the present invention is 40-50%, which is a relatively suitable concentration. It is used for soaking small-leaf red bean seeds, which can further improve the bactericidal effect and avoid damage to the seeds due to too high concentration or failure to achieve the expected bactericidal effect due to too low concentration. Therefore, fast emergence and high germination rate can be achieved.

[0038] In the specific operation of the present invention, the probiotic aqueous solution can be used as a boundary to cover the surface of the seeds, so as to better soak the seeds for sterilization.

[0039] In some embodiments of the present invention, the pH value of the substrate for hardening culture is 5.5-6.0.

[0040] The pH value of the substrate for seedling hardening and cultivation in the present invention is in a suitable range, which is conducive to the growth and development of plant root system, makes the root system stronger, can absorb nutrients and moisture better, and makes the growth rate of plants faster. In addition, within this pH value range, many trace elements (such as iron, manganese, zinc, etc.) necessary for plants can exist in a form that is easy to absorb by plants, thereby improving the utilization rate of nutrients by plants. Therefore, the substrate of the suitable pH value range can further achieve fast emergence and high germination rate.

[0041] In some embodiments of the present invention, the substrate for acclimatization is prepared by a method comprising the following steps:

[0042] The substrate is mixed with a sulfur powder solution with a mass concentration of 10-20%, heated at 121° C. for 20-50 minutes, cooled, and air-dried to a water content of 20-30% to obtain the substrate for seedling hardening culture.

[0043] The present invention improves the acidity of the substrate for seedling hardening culture. Specifically, the substrate is mixed with a sulfur powder solution, wherein the mass concentration of the sulfur powder solution can be 10-20%. Since the sulfur powder is acidic, after being mixed with the substrate and heated for a certain period of time, the pH value of the substrate can be adjusted to a range more suitable for plant growth. The substrate is air-dried to a water content of 20-30%, which helps to maintain a suitable humidity of the substrate during the seedling hardening process, and neither causes root rot due to excessive moisture in the substrate nor affects the normal growth of the plant due to excessive drying. The stability and durability of the substrate are enhanced, so that the substrate is not easily broken or deformed during use, and is conducive to maintaining the stable growth of the plant root system.

[0044] The invention simulates the acidic growth environment of small-leaf red beans by improving the acidity of the substrate for seedling hardening culture, which is more conducive to the growth of the small-leaf red beans and realizes fast emergence and high germination rate.

[0045] In some embodiments of the present invention, the volume ratio of the matrix to the sulfur powder solution is 2-3:1-2.

[0046] The volume ratio of the substrate to the sulfur powder solution is limited to 2-3:1-2 in the present invention. The pH value of the substrate can be adjusted within a suitable range to make it more suitable for plant growth, further achieving fast emergence and high germination rate.

[0047] In some embodiments of the present invention, the substrate comprises: peat, perlite, wood chips, and fine sand in a volume ratio of 2-3:1:1:1-3; and / or,

[0048] The culture solution used for the strong seedling culture comprises: 0.1-0.5 mg / L 6-BA and 0.1-0.5 mg / L NAA.

[0049] The peat in the composition of the matrix of the present invention is rich in organic matter and trace elements, which can slowly release nutrients and provide long-term and stable nutritional support for plants. The wood chips can also provide certain nutrients for plants after decomposition and help improve the soil structure. In addition, peat and wood chips have good water retention capacity, which can keep the moisture in the matrix stable and reduce the problem of plant water shortage caused by too fast evaporation of water. The addition of perlite and fine sand can enhance the air permeability of the matrix, which is beneficial to the respiration of plant roots and reduces the occurrence of root diseases.

[0050] The 6-BA in the culture solution used for the seedling cultivation of the present invention is a cytokinin that can promote the division and proliferation of plant cells and accelerate the growth rate of plants. The appropriate concentration of 6-BA can make the plant root system well-developed, the stems thick and the leaves dark green, and improve the overall growth potential of the plant. NAA is an auxin analog that has the effect of promoting plant rooting. In the seedling cultivation, NAA can promote the development of the plant root system, improve the absorption capacity and stress resistance of the root system, and make the seedlings stronger. The appropriate concentrations of 6-BA and NAA can achieve effective regulation of plant growth.

[0051] The composition of the substrate and the culture solution used for seedling cultivation in the present invention can promote the growth of plants and the development of the root system, and can further achieve rapid emergence of seedlings and high germination rate.

[0052] Furthermore, the matrix includes peat, perlite, wood chips and fine sand in a volume ratio of 2-3:1:1:1-3, which can better provide nutrients for plants, maintain the stability of matrix moisture, enhance the air permeability of the matrix, and further achieve fast seedling emergence and high germination rate.

[0053] Furthermore, the culture solution used for the seedling cultivation includes: 0.3 mg / L 6-BA, 0.3 mg / L NAA, which can better promote plant growth, promote root development, and achieve fast seedling emergence and high germination rate.

[0054] In some embodiments of the present invention, the germination culture comprises: culturing the activated seeds in a dark culture room at 18-25° C. for 1-2 days, and then transferring them to a light culture room at 18-25° C. with a light intensity of 1800-2500 lx to obtain 18-25 cm small-leaf red bean seedlings; and / or,

[0055] The temperature of the seedling cultivation is 18-25°C and the light intensity is 1800-2500lx; and / or,

[0056] The temperature of the seedling hardening culture is 22-30° C., and the light intensity is 2500-3000 lx.

[0057] The germination culture of the present invention can be performed by placing activated seeds flatly in a germination box (with a cover) whose bottom is kept moist, wherein the bottom paper is the paper of the germination box, sprayed with water to ensure humidity, and first placed in a dark culture room at 18-25°C for culture for 1-2 days, the seeds swell, and then transferred to a light culture room at 18-25°C with a light intensity of 1800-2500lx for culture, the seeds turn white in about 3 days, some of the seeds have radicles, the cotyledons grow in about 5 days, and small-leaf red bean seedlings of 18-25cm can be obtained in about 7-9 days, and the proportion of small-leaf red bean seedlings of 18-25cm is 99%.

[0058] The seedling cultivation of the present invention can be carried out by spraying growth hormones on the roots and axillary buds of uniform and uniform small-leaf red bean seedlings of 18-25 cm every day, irrigating the roots, and carrying out seedling cultivation treatment for 10 consecutive days, with 10 small-leaf red bean seedlings in each group, repeating three times, the temperature of the culture room is 18-25°C, and the light intensity is 1800-2500lx.

[0059] The seedling hardening culture in the present invention can firstly be carried out by filling a seedling pot with a hole diameter of 5cm×5cm with a substrate of 2 / 3 of the height of the seedling pot, and then planting the neat and consistent 18-25cm seedlings after seedling cultivation into the sterilized substrate. During the planting process, the roots are laid flat without damaging the roots, and watered thoroughly to ensure that the roots are dry and wet. The seeds are placed in a greenhouse with a temperature of 22-30°C and a light intensity of 2500-3000lx. The root base swells and the true leaves unfold in about 7 days, and the plants take root in about 10 days. The rooting rate is greater than 85%.

[0060] The conditions of the germination culture, seedling hardening culture and seedling hardening culture of the present invention can further achieve the goals of rapid seedling emergence and high germination rate.

[0061] The technical solution of the present invention is further described below in conjunction with specific embodiments.

[0062] Example 1

[0063] The propagation method of the small leaf red bean of the present embodiment comprises the following steps:

[0064] 1) The test material seeds were small-leaved red bean seeds stored in a 4℃ seed storage cabinet of the Yangtze River Rare Plants Institute for 5 years. After taking out the seeds, put them into a bucket filled with clean water, remove the unripe seeds and empty seeds floating on the water surface, and select the seeds with complete appearance, full size, uniform grains, and no mold, rot, diseases or insect pests from the seeds that sink to the bottom.

[0065] 2) Pour the seeds into a 45% mass concentration of Bacillus licheniformis solution and soak them for 2 hours, sterilize them until the probiotic solution covers the surface of the seeds, and the ambient temperature is 4-10°C to obtain soaked seeds. After washing, heat them in a 50°C oven for 2 hours to obtain activated seeds.

[0066] 3) Place the seeds flat in a germination box (with a lid) with the bottom kept moist. The bottom paper is the paper provided by the germination box. Spray water on the seeds to ensure humidity. Place the seeds in a dark culture room at 18-25°C for 1 day. Seed swelling is found. Then the seeds are transferred to a light culture room at 18-25°C and 1800-2500lx. It is observed that the seeds turn white in 3 days, some have radicles, and cotyledons grow in 5 days. Small-leaf red bean seedlings of 18-25 cm can be obtained in 7 days, and the proportion of small-leaf red bean seedlings of 18-25 cm is 99%.

[0067] 4) Every day, the roots and axillary buds of the 18-25 cm uniform small-leaf red bean seedlings were sprayed with culture solution, and the roots were irrigated to strengthen the seedlings. This process was repeated three times for 10 consecutive days. The temperature in the culture room was 18-25°C, the light intensity was 1800-2500lx, and the culture solution included: 0.3mg / L6-BA, 0.3mg / L NAA. It was observed that the plants were strong and the leaves were dark green.

[0068] 5) Mix the substrate of peat, perlite, sawdust and fine sand in a volume ratio of 2:1:1:2 with a sulfur powder solution of 20% by mass concentration, wherein the volume ratio of the substrate to the sulfur powder solution is 3:2, heat at 121℃ for 30min, cool, adjust the pH value to 5.6, and air-dry to a water content of 25% for later use. Prepare a clean 5cm×5cm aperture seedling pot, put the sterilized substrate into it, and fill it to a height of 2 / 3 of the seedling pot for later use, and then plant the neat and consistent 18-25cm strong seedlings of small-leaf red bean seedlings into the sterilized substrate. During the planting process, lay the roots flat, do not damage the roots, and water thoroughly to see dry and wet. Place in a greenhouse with a temperature of 18-25℃ and a light intensity of 1800-2500lx. There are 15 plants in each group, repeated 3 times, and observed once every 7 days. About 7 days after potting, the root base will swell and the true leaves will unfold. About 10 days later, the plant will grow roots. The root growth indicator is that the plant cannot be pulled out when gently shaken. After 30 days, the rooting situation of the small-leaf red bean is counted, and the results are shown in Table 1.

[0069] Example 2

[0070] The propagation method of the small-leaf red bean in Example 2 is basically the same as that in Example 1, except that the probiotic used in step 2) is Bacillus subtilis.

[0071] Example 3

[0072] The propagation method of the small-leaf red bean in Example 3 is basically the same as that in Example 1, except that the concentration of the Bacillus licheniformis solution in step 2) is 50%.

[0073] Example 4

[0074] The propagation method of the small-leaf red bean in Example 4 is basically the same as that in Example 1, except that the pH value of the substrate for seedling hardening culture in step 5) is 6.5.

[0075] Example 5

[0076] The propagation method of the small-leaf red bean in Example 5 is basically the same as that in Example 1, except that the volume ratio of the substrate to the sulfur powder solution in step 5) is 3:1.

[0077] Example 6

[0078] The propagation method of the small-leafed red bean in Example 6 is basically the same as that in Example 1, except that in step 5), the matrix is ​​peat, perlite, wood chips, and fine sand in a volume ratio of 3:1:1:2.

[0079] Example 7

[0080] The propagation method of the small-leafed red bean in Example 7 is basically the same as that in Example 1, except that in step 5), the matrix is ​​peat, perlite, wood chips, and fine sand in a volume ratio of 3:1:1:1.

[0081] Example 8

[0082] The propagation method of the small-leafed red bean in Example 8 is basically the same as that in Example 1, except that in step 5), the matrix is ​​peat, perlite, wood chips, and fine sand in a volume ratio of 2:1:1:1.

[0083] Example 9

[0084] The propagation method of Example 9 is basically the same as that of Example 1, except that the culture solution in step 4) includes: 0.5 mg / L 6-BA, 0.5 mg / L NAA. It can be observed that the stem segments of the seedlings after the seedling cultivation become thicker, but the leaf color is yellowish.

[0085] Example 10

[0086] The propagation method of the small-leaf red bean in Example 10 is basically the same as that in Example 1, except that the culture solution in step 4) includes: 0.1 mg / L 6-BA, 0.1 mg / L NAA. It can be observed that the leaves of the seedlings after the strong seedling cultivation are light yellow, and the plants are weaker than those in Example 1.

[0087] Embodiment 11

[0088] The propagation method of Example 11 is basically the same as that of Example 1, except that the culture solution in step 4) includes 0.5 mg / L 6-BA. It can be observed that there is no change in the stem segments of the seedlings after the seedling cultivation, and only some axillary buds sprout.

[0089] Example 12

[0090] The propagation method of Example 12 is basically the same as that of Example 1, except that the culture solution in step 4) includes: 0.5 mg / L NAA. It can be observed that fibrous roots sprout at the base of the seedlings after seedling cultivation, but the amount is small and the leaf color is yellowish.

[0091] Embodiment 13

[0092] The propagation method of the small-leaf red beans in Example 13 is basically the same as that in Example 1, except that in step 5), the sulfur powder solution is not used to acidify the substrate.

[0093] Embodiment 14

[0094] The propagation method of the small-leaf red beans in Example 14 is basically the same as that in Example 1, except that the temperature of the heating treatment in step 1) is 40°C.

[0095] Embodiment 15

[0096] The propagation method of the small-leaf red beans in Example 15 is basically the same as that in Example 1, except that the temperature of the heating treatment in step 1) is 60°C.

[0097] Comparative Example 1

[0098] The propagation method of the small-leaf red beans in Comparative Example 1 is basically the same as that in Example 1, except that the seeds are not soaked in step 2).

[0099] Comparative Example 2

[0100] The propagation method of the small-leaf red beans in Comparative Example 2 is basically the same as that in Example 1, except that the soaked seeds are not heated in step 2).

[0101] Comparative Example 3

[0102] The propagation method of the small-leaf red beans in Comparative Example 3 is basically the same as that in Example 1, except that in step 2), the seeds are not soaked and the soaked seeds are not heated.

[0103] Comparative Example 4

[0104] Comparative Example 4 The propagation method of small-leaf red beans is basically the same as that of Example 1, except that in step 2), 500 times of 50% carbendazim solution is used to soak the seeds.

[0105] Table 1

[0106]

[0107]

[0108] As can be seen from Table 1, the propagation method of small-leaf red bean provided by the present invention is to soak the small-leaf red bean seeds in a probiotic aqueous solution for sterilization, and to heat the soaked seeds to break the dormancy of the small-leaf red bean seeds and promote germination, thereby achieving the goals of fast emergence and high germination rate.

[0109] From the comparison between Example 1 and Comparative Examples 1-4, it can be seen that the small-leaf red bean seeds are soaked in a probiotic aqueous solution for sterilization, and the soaked seeds are heated to break the dormancy of the small-leaf red bean seeds and promote germination, thereby achieving the goals of fast emergence and high germination rate.

[0110] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for propagating small-leaf red beans, characterized in that: The following steps are involved: 1) soaking red bean seeds in a probiotic aqueous solution for 2-4 hours to obtain soaked seeds; 2) heating the soaked seeds at 40-60° C. for 2-4 hours to obtain activated seeds; 3) The activated seeds are subjected to germination culture, seedling hardening culture and seedling hardening culture in sequence to obtain rooted seedlings of small-leaf red beans.

2. The breeding method according to claim 1, characterized in that The probiotics include at least one of Bacillus subtilis, Bacillus licheniformis and Bacillus megaterium.

3. The breeding method according to claim 2, characterized in that The concentration of the probiotic aqueous solution is 40-50%.

4. The breeding method according to claim 1, characterized in that The pH value of the substrate for the seedling hardening culture is 5.5-6.

0.

5. The breeding method according to claim 4, characterized in that: The substrate for acclimatization is prepared by a method comprising the following steps: The substrate is mixed with a sulfur powder solution with a mass concentration of 10-20%, heated at 121° C. for 20-50 minutes, cooled, and air-dried to a water content of 20-30% to obtain the substrate for seedling hardening culture.

6. The breeding method according to claim 4, characterized in that: The volume ratio of the matrix to the sulfur powder solution is 2-3:1-2.

7. The breeding method according to claim 6, characterized in that: The matrix comprises: peat, perlite, wood chips, and fine sand in a volume ratio of 2-3:1:1:1-3; and / or, The culture solution used for the strong seedling culture comprises: 0.1-0.5 mg / L 6-BA and 0.1-0.5 mg / L NAA.

8. The breeding method according to claim 7, characterized in that: The matrix includes: peat, perlite, wood chips and fine sand in a volume ratio of 2:1:1:

2.

9. The breeding method according to claim 1, characterized in that: The culture solution used for the strong seedling culture includes: 0.3 mg / L 6-BA and 0.3 mg / L NAA.

10. The breeding method according to any one of claims 1 to 9, characterized in that: The germination culture comprises: culturing the activated seeds in a dark culture room at 18-25° C. for 1-2 days, and then transferring them to a light culture room at 18-25° C. and a light intensity of 1800-2500 lx to obtain 18-25 cm small-leaf red bean seedlings; and / or, The temperature of the seedling cultivation is 18-25°C and the light intensity is 1800-2500lx; and / or, The temperature of the seedling hardening culture is 22-30° C., and the light intensity is 2500-3000 lx.

Images