Method for promoting rooting of rhododendron lapponicum tissue culture seedlings difficult to root

Abstract

The invention relates to a method for promoting rooting of Rhododendron lapponicum tissue culture seedlings difficult to root, which comprises the following steps: cutting proliferation seedlings into single plants with the height of 2-3cm, inoculating the single plants into a transition culture medium which is WPM with 1 g / L of active carbon, and culturing at 23-25 DEG C for 35-40 days; the plantlets are transferred to a rooting induction culture medium, the rooting induction culture medium is WPM, 1 mg / L of NAA and 0.75 mg / L of IBA are added to the WPM, and the plantlets are cultured for 30-40 days at the temperature of 23-25 DEG C; 30 g / L of sugar and 6 g / L of agar powder are added into the culture medium, the pH value is adjusted to 5.8, and the illumination intensity is 2500-3000 lux. According to the method for promoting rooting of the Rhododendron lapponicum 'Promisius' tissue culture seedling difficult to root, the rooting rate can be effectively increased, robust growth of the seedling is ensured, the rooting problem in Rhododendron lapponicum 'Promisius' seedling production is solved, and a new technical support is provided for application and popularization of the Rhododendron lapponicum 'Promisius' seedling.

Description

Technical Field

[0001] The invention belongs to the technical field of plant tissue culture and relates to a method for promoting root growth of difficult-to-root alpine rhododendron tissue culture seedlings. Background Art

[0002] As a world-famous precious woody flower, Rhododendron alpinum has extremely high ornamental value in gardening and landscaping. Rhododendron alpinum 'Prometheus' is an excellent evergreen variety of this species, with large corolla and bright colors, and is widely used in the flower industry. However, due to the low rooting rate and poor root development during tissue culture, especially in the transplanting stage, the large-scale production of this variety is limited.

[0003] In order to obtain a higher propagation coefficient, a certain concentration of cytokinin is often added to the culture medium during the proliferation stage of tissue culture seedlings. Although this promotes the proliferation of tissue culture seedlings to a certain extent, it affects the rooting efficiency of some varieties. Tissue culture seedlings of Rhododendron 'Prometheus' are usually difficult to root normally due to long-term exposure to high concentrations of cytokinin (such as ZT or 6-BA) and other hormones. The rooting rate is often close to zero, resulting in an extremely low survival rate of transplanted tissue culture seedlings, which further limits the promotion and application of this variety.

[0004] Therefore, how to effectively improve the rooting rate of the difficult-to-root alpine Rhododendron 'Prometheus' tissue culture seedlings and promote the robust growth of its root system has become a key issue in the large-scale production and promotion of this variety. Summary of the invention

[0005] The purpose of the present invention is to provide a method for effectively promoting the rooting of the difficult-to-root alpine azalea 'Prometheus' tissue culture seedlings. The method optimizes the rooting culture steps and adds a transition culture link, while balancing the plant's endogenous hormones, promotes the rooting of the alpine azalea 'Prometheus' tissue culture seedlings and improves the rooting rate, and finally realizes the large-scale production and promotion of the seedlings of this variety.

[0006] According to the purpose of the present invention, the present invention provides a method for promoting root growth of difficult-to-root alpine rhododendron tissue culture seedlings, comprising the following steps:

[0007] S1. Transitional training steps:

[0008] Cut the propagated seedlings into individual plants with a height of 2-3 cm and inoculate them into the transition medium;

[0009] The transition medium was WPM (Woody Plant Medium) with 1 g / L activated carbon added;

[0010] Cultivate in this medium for 35-40 days;

[0011] S2, rooting induction culture steps:

[0012] The seedlings after transitional culture were transferred to the rooting induction medium, the medium formula of which was WPM supplemented with 1 mg / L NAA (naphthaleneacetic acid) and 0.75 mg / L IBA (indolebutyric acid);

[0013] Continue culturing in the medium for 30-40 days;

[0014] This method can achieve a rooting rate of over 65%, and the rooted seedlings are strong.

[0015] Furthermore, in S1 and S2, the culture medium formula and culture conditions are:

[0016] Sugar 30 g / L and agar powder 6 g / L were added to both the transition medium and the root induction medium;

[0017] pH adjusted to 5.8;

[0018] The temperature of the culture room was maintained at 23-25°C and the light intensity was 2500-3000 lux.

[0019] Beneficial effects of the present invention:

[0020] The present invention discloses a method for promoting rooting of alpine azalea tissue culture seedlings with difficulty in rooting. By optimizing the rooting culture steps and adding a transition culture link, the method can effectively promote the rooting of alpine azalea 'Prometheus' tissue culture seedlings and significantly improve the rooting rate. Thus, the tissue culture seedlings of this variety have stronger rooting ability and a strong root system when transplanted, and the survival rate is greatly improved, which provides technical guarantee for the large-scale production and promotion of alpine azalea 'Prometheus'.

[0021] Compared with the traditional alpine rhododendron tissue culture seedling cultivation method, the present invention can effectively overcome the adverse effects of long-term use of high-concentration cytokinins on rooting, promote the normal development of plant roots through fine hormone regulation and balance of endogenous hormones, and ensure the healthy growth of seedlings. DETAILED DESCRIPTION

[0022] The specific embodiments of the present invention are further described below. It should be noted that the description of these embodiments is used to help understand the present invention, but does not constitute a limitation of the present invention. In addition, the technical features involved in each embodiment of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0023] Example 1

[0024] A method for promoting root growth of difficult-to-root alpine rhododendron tissue culture seedlings, comprising the following steps:

[0025] The propagated seedlings were cut into individual plants with a height of 2-3 cm and inoculated into the transition medium;

[0026] The culture medium was WPM supplemented with activated carbon 1 g / L, and the culture was carried out for 40 days;

[0027] After 40 days, the seedlings were transferred to root induction medium, the medium formula of which was WPM supplemented with 1 mg / L NAA and 0.75 mg / L IBA;

[0028] Continue to cultivate for 30-40 days, and finally obtain a rooting rate of more than 65%, and the rooted seedlings are strong;

[0029] 30 g / L sugar and 6 g / L agar powder were added to the culture medium, and the pH value was adjusted to 5.8;

[0030] The temperature of the culture room is 23-25℃, and the light intensity is 2500-3000lux.

[0031] Example 2

[0032] A method for promoting root growth of difficult-to-root alpine rhododendron tissue culture seedlings, comprising the following steps:

[0033] The propagated seedlings were cut into individual plants with a height of 2-3 cm and inoculated into the transition medium;

[0034] The culture medium was WPM supplemented with activated carbon 1 g / L, and the culture was carried out for 38 days;

[0035] After 38 days, the seedlings were transferred to root induction medium, the medium formula of which was WPM supplemented with 1 mg / L NAA and 0.75 mg / L IBA;

[0036] After 38 days of cultivation, the rooting rate was over 65% and the rooted seedlings were strong.

[0037] 30 g / L sugar and 6 g / L agar powder were added to the culture medium, and the pH value was adjusted to 5.8;

[0038] The temperature of the culture room is 23-25℃, and the light intensity is 2500-3000lux.

[0039] Example 3

[0040] A method for promoting root growth of alpine rhododendron tissue culture seedlings, comprising the following steps:

[0041] The propagated seedlings were cut into individual plants with a height of 2-3 cm and inoculated into the rooting medium;

[0042] The medium formula was WPM supplemented with 1 mg / L NAA, 0.75 mg / L IBA, and 1 g / L activated carbon;

[0043] After 70 days of cultivation, a rooting rate of 4% was finally obtained;

[0044] 30 g / L sugar and 6 g / L agar powder were added to the culture medium, and the pH value was adjusted to 5.8;

[0045] The temperature of the culture room is 23-25℃, and the light intensity is 2500-3000lux.

[0046] The present invention discloses a method for promoting rooting of alpine azalea tissue culture seedlings with difficulty in rooting. By optimizing the rooting culture steps and adding a transition culture link, the rooting of alpine azalea 'Prometheus' tissue culture seedlings with difficulty in rooting can be effectively promoted, and the rooting rate can be significantly improved. Thus, the tissue culture seedlings of this variety have stronger rooting ability and a robust root system when transplanted, and the survival rate is greatly improved, which provides technical guarantee for the large-scale production and promotion of alpine azalea 'Prometheus'.

[0047] Compared with the traditional alpine rhododendron tissue culture seedling cultivation method, the present invention can effectively overcome the adverse effects of long-term use of high-concentration cytokinins on rooting, promote the normal development of plant roots through fine hormone regulation and balance of endogenous hormones, and ensure the healthy growth of seedlings.

[0048] The method for promoting rooting of the difficult-to-root alpine rhododendron 'Prometheus' tissue culture seedlings provided by the present invention can effectively improve the rooting rate, ensure the robust growth of the seedlings, solve the rooting problem of the alpine rhododendron 'Prometheus' in large-scale production, and provide new technical support for the application and promotion of this variety.

[0049] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for promoting root growth of difficult-to-root alpine rhododendron tissue culture seedlings, characterized in that: The following steps are involved: The propagated seedlings were cut into individual plants with a height of 2-3 cm, and inoculated into a transition medium, wherein the transition medium was WPM supplemented with 1 g / L activated carbon, and cultured at 23-25° C. for 35-40 days; The seedlings were transferred to a rooting induction medium, wherein the rooting induction medium was WPM supplemented with 1 mg / L NAA and 0.75 mg / L IBA, and cultured at 23-25° C. for 30-40 days; Add 30g / L sugar and 6g / L agar powder to the culture medium, adjust the pH value to 5.8, and the light intensity to 2500-3000lux.

2. The method according to claim 1, characterized in that Transition culture is required before rooting induction.

3. The method according to claim 1, characterized in that Only activated carbon was added to the transition culture medium, and the concentration was 1 g / L.

4. The method according to claim 1, characterized in that The temperature of the culture chamber is 23-25°C.

5. The method according to claim 1, characterized in that The seedlings are cultured in the transition medium for 35-40 days, and in the rooting induction medium for 30-40 days.

6. The method according to claim 1, characterized in that The concentration of NAA in the root induction medium is 1 mg / L, and the concentration of IBA is 0.75 mg / L.

7. The method according to claim 1, characterized in that The pH value of the rooting induction medium is adjusted to 5.8.