Artificial breeding method for acanthocybe chlamydoides

Abstract

The invention provides an artificial breeding method for echinacea chlamydoides. The artificial breeding method comprises the following steps: S1, selecting parent fish; s2, performing nutrient enrichment culture on the parent fish; s3, artificial spawning and fertilization; s4, artificial incubation; s5, seedling cultivation. The feed is reasonably matched to promote parent fish development and improve egg laying and sperm production quality, spawning hormone is manually injected, injection dosage is strictly controlled, eggs and sperm which are well developed are obtained, hatching conditions of the fertilized eggs are controlled, the hatching rate of the fertilized eggs reaches 90% or above, and the hatching rate of the fertilized eggs reaches 90% or above. According to the method, oyster fertilized eggs, rotifers, artemia or cladocerans / copepods nauplii, cladocerans / copepods and floating type larva fry breeding seawater fish compound feed are sequentially fed in the fry breeding period, palatable bait series of different fry breeding stages are established, and a mature fry breeding technical route is formed.

Description

Technical Field

[0001] The invention relates to the technical field of fish breeding, and in particular to an artificial breeding method for gray-finned seabream. Background Art

[0002] Acanthopagrus berda, formerly known as Sparus berda Forskal, belongs to the genus Acanthopagrus of the family Sparidae of the order Perciformes. It is commonly known as black bull, black bream, flying bream, black fin, and cow dung bream. It is a coastal bottom-dwelling, euryhaline fish. It often inhabits sea areas with rocky reefs, boulders, or sandy mud bottoms at a depth of 3 to 50 meters. Sometimes it also enters oyster sheds, mangroves, or wave-breaking blocks in embankment areas at brackish and freshwater estuaries or moves in rivers. It is omnivorous and feeds on shellfish, crustaceans, small fish, algae, organic debris, mainly algae and small benthic animals. The reproductive period is from winter to spring. Natural groups generally gather at the river estuary to spawn. In spring, young fish begin to appear in the estuary and can swim upstream to freshwater areas. In my country, it is mainly distributed in the South China Sea and the Taiwan Strait, and abroad, it is distributed in the Indian Ocean, the east coast of Africa, the Red Sea, east to Australia, and north to Japan. Traditional fishing methods are mainly hand fishing, purse seine, and drift net.

[0003] At present, the research on gray-finned seabream in China is relatively scattered. In terms of breeding, there is only an experimental study on "hybrid breeding of black seabream and gray-finned seabream" by Lin Yuanshao et al. of Xiamen University (1999-2001), which compared the fertilization rate, hatching rate and survival rate of fry and juvenile fish of the positive and negative cross combinations. In terms of breeding, Zhang Bangjie et al. of Dongguan Fisheries Research Institute conducted a benefit analysis of small-scale pond polyculture of seabream in "Study on pond culture technology of cod and seabream in estuary and nearshore" (1997). In addition, Chen Yongxia et al. of the School of Life Sciences of Hebei University conducted "Systematic evolutionary relationship of Chinese seabream based on mitochondrial COI gene sequence" (2015), and Jiang Shigui, Liu Hongyan et al. of the Chinese Academy of Fishery Sciences and the South China Sea Fisheries Research Institute conducted "Mitochondrial cytochrome b gene sequence and molecular systematic analysis of four species of seabream" (2003), mainly using mitochondrial COI gene sequence to analyze the systematic relationship between species, subspecies and geographical populations of seabream. However, there is no report on the artificial breeding technology of gray-finned seabream abroad. Summary of the invention

[0004] In view of this, the present invention provides an artificial breeding method for gray-finned seabream. The present invention promotes the development of parent fish and improves the quality of spawning and sperm production by reasonably matching feeds, obtains well-developed eggs and semen by artificially injecting oxytocin and strictly controlling the injection dosage, and controls the hatching conditions of fertilized eggs at the same time, so that the hatching rate of fertilized eggs reaches more than 90%. The present invention feeds oyster fertilized eggs-rotifers-artemia or cladoceran / copepod nauplii-cladoceran / copepoda-floating type fry and juvenile marine fish compound feed in sequence during seedling cultivation, establishes a series of palatable baits for different stages of seedling cultivation, and forms a relatively mature seedling cultivation technology route.

[0005] A method for artificial breeding of grey-finned seabream comprises the following steps:

[0006] S1. Selection of broodstock:

[0007] The wild grey-finned seabream is transported to the domestication pond, and healthy individuals with no external injuries on the body surface, normal body shape and no disabilities are selected as broodstock for future use;

[0008] S2. Nutritional enhancement of broodstock:

[0009] After being fed with live seawater or freshwater shrimp for 10-15 days, the fish are fed with fresh ice fish or shellfish for 10-15 days, and finally transitioned to being fed with homemade feed for nutritional fortification. The homemade feed is a formula with added fish paste, eel meal, multivitamins and probiotics. The daily feeding amount is 3.0-5.0% of the fish body weight, and the fish are fed 1-2 times a day. Wild grayfin seabream are fed until the gonads mature, and the fish are used as breeding broodstock for standby use;

[0010] S3. Artificial insemination:

[0011] Injecting oxytocin, luteinizing hormone-releasing hormone and chorionic gonadotropin into the breeding broodstock in step S2 for artificial induction of labor, wherein the injection dose of the luteinizing hormone-releasing hormone is 5.0-10.0 μg / kg, the injection dose of the chorionic gonadotropin is 300-1000 IU / kg, the male-female ratio is 1:1, and each batch of breeding broodstock is injected 3-5 times, each time at an interval of 24-36 hours, and the breeding broodstock naturally spawns and fertilizes to obtain fertilized eggs;

[0012] S4. Artificial incubation:

[0013] The fertilized eggs in step S3 are placed in an incubation bucket for artificial incubation until hatching, thereby obtaining gray-finned seabream fry;

[0014] S5. Seedling cultivation:

[0015] The grayfin thorny seabream fry described in step S4 is transferred to a cultivation pond, and food is started to be fed after the third day of hatching until grayfin thorny seabream fry is obtained.

[0016] Preferably, in the initial stage of domestication in step S1, the domestication pool is disinfected with a disinfectant, once every 2-3 days, for 2-3 consecutive times, and then once every 15 days. During the disinfection period, the water is aerated, and domestication is carried out after 24 hours with running water aeration.

[0017] Preferably, the injection dose of the male breeding broodstock in step S3 is half of the injection dose of the female breeding broodstock.

[0018] Preferably, the injection site in step S3 is the dorsal fin or pectoral fin of the breeding broodstock.

[0019] Preferably, the environmental conditions for artificial induction of labor and fertilization in step S3 are water temperature 20.0-25.5° C., DO 6.89-9.77 mg / l, pH 7.791-8.022, and salinity 27.8-29.1.

[0020] Preferably, the density of the fertilized eggs in step S4 is 150-200 eggs / L, the incubation conditions are water temperature 21.8°C, DO 7.58 mg / l, pH 7.69, salinity 27.9, and the incubation time is 18-22 hours.

[0021] Preferably, the grayfin seabream fry in step S4 have an average total length of 1.66 mm and a fertilized egg hatching rate of 93.5%.

[0022] Preferably, step S5 includes a larva-juvenile stage and a juvenile-fledgling stage.

[0023] Preferably, in the larvae-juvenile stage, the unicellular algae are fed for static water cultivation and oxygenation, and the larvae density is 10,000 to 15,000 fish / m 3 , the density of unicellular algae is 100-200 / ml, the water temperature is maintained at 21.4-23.6°C, the salinity (SAL) is 27.9-29.4, the pH value is 7.706-7.901, and the dissolved oxygen (DO) is 6.79-7.26 mg / L during the cultivation stage. Ostreidae fertilized eggs and larvae are fed on the third day after hatching, followed by Brachionus sp., and an appropriate amount of Artemia (Brine shrimp) is fed every day after 10 days. Shrimp) or small copepods (Copepoda) and their nauplii, the content of the oyster fertilized eggs and their larvae is 2.0-4.0 pieces / ml, the density of the rotifer is 0.8-2.0 pieces / ml, and the density of the Artemia or small copepods and their nauplii is 0.05-1.5 pieces / ml; after 20 days, the fry and juvenile fish seedlings are fed with a compound feed, and after 30 days, all the fry and juvenile fish are fed with a compound feed, 3-4 times a day, and the feeding amount is based on the feeding activity and satiety of the fry observed with the naked eye;

[0024] Preferably, the juvenile-juvenile stage is cultivated in a micro-flow water manner with oxygenation, and the juvenile density is 0.5-10,000 / m 3 , juvenile density 0.04~0.50 thousand / m 3 The water change rate is 10-20% per day, the water temperature during the cultivation period is 22.7-24.6℃, the salinity (SAL) is 28.5-29.3, the pH value is 7.706-7.901, the dissolved oxygen content (DO) is 6.50-7.13mg / L, and the fish are fed with marine fish compound feed, 3-4 times a day, and the feeding amount is 30-50g / 10,000 fish.

[0025] Compared with the prior art, the present invention has the following beneficial effects: the present invention moves the collected wild gray-finned seabream to an indoor domestication pond for artificial domestication in a simulated ecological mode, feeds shrimps, shellfish, miscellaneous fish and compound feeds with added nutrient enhancers during the domestication process to cultivate the broodstock until the gonads are mature; then artificial induction of spawning is performed on the broodstock with mature gonads by injecting a certain dose of oxytocin LRH-A3 and HCG, with a male-female ratio of 1:1, so as to achieve natural spawning and fertilization; the fertilized eggs are placed in an incubation bucket for aeration incubation, and the hatched fry are moved into an indoor cultivation pond for static water cultivation with water rich in unicellular algae and rotifers that has been cultured in advance, and oxygen is increased by aeration using a Roots blower, and during the period, the oyster fertilized eggs-rotifers-artemia or cladoceran / copepod nauplii-cladoceran / copepoda-floating fry and juvenile marine fish compound feeds are fed in sequence, so as to establish a series of palatable baits for different stages of seed cultivation, and establish a relatively mature seed cultivation technical route. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 The invention discloses a technical flow chart of the artificial breeding method of grayfin thorny seabream. DETAILED DESCRIPTION

[0027] In the present invention, a method for artificial breeding of grayfin porgy comprises the following steps:

[0028] S1. Selection of broodstock:

[0029] The wild grey-finned seabream is transported to the domestication pond, and healthy individuals with no external injuries on the body surface, normal body shape and no disabilities are selected as broodstock for future use;

[0030] S2. Nutritional enhancement of broodstock:

[0031] After being fed with live seawater or freshwater shrimp for 10-15 days, the fish are fed with fresh ice fish or shellfish for 10-15 days, and finally transitioned to being fed with homemade feed for nutritional fortification. The homemade feed is a formula with added fish paste, eel meal, multivitamins and probiotics. The daily feeding amount is 3.0-5.0% of the fish body weight, and the fish are fed 1-2 times a day. Wild grayfin seabream are fed until the gonads mature, and the fish are used as breeding broodstock for standby use;

[0032] S3. Artificial insemination:

[0033] Injecting oxytocin luteinizing hormone releasing hormone and chorionic gonadotropin into the breeding broodstock in step S2 for artificial induction of labor, wherein the injection dose of the luteinizing hormone releasing hormone is 5.0-10.0 μg / kg, the injection dose of the chorionic gonadotropin is 300-1000 IU / kg, the male-female ratio is 1:1, and each batch of breeding broodstock is injected 3-5 times, each time interval is 24-36 hours, and the breeding broodstock spawns and fertilizes naturally to obtain fertilized eggs, and the fertilization rate is 75.0-84.2%;

[0034] S4. Artificial incubation:

[0035] The fertilized eggs in step S3 are placed in an incubation bucket for artificial incubation until hatching, thereby obtaining gray-finned seabream fry;

[0036] S5. Seedling cultivation:

[0037] The grayfin thorny seabream fry described in step S4 is transferred to a cultivation pond, and food is started to be fed after the third day of hatching until grayfin thorny seabream fry is obtained.

[0038] In the present invention, in step S1, at the initial stage of domestication, the domestication pool is disinfected with a disinfectant, once every 2-3 days, 2-3 times continuously, and then once every 15 days, during which the water is aerated, and the fish are domesticated after 24 hours of aeration with running water. Furthermore, at the initial stage of domestication, a glutaraldehyde solution with a concentration of 0.2ppm is sprinkled on the domestication pool, and the whole pool is bathed in medicine to prevent bacterial diseases caused by fish skin damage.

[0039] In the present invention, the proportions of the components in the homemade feed in step S2 are 62.18% of fish paste, 37.62% of eel meal, 0.1% of multivitamins and 0.1% of probiotics.

[0040] In the present invention, the eel powder in step S2 is the "Seahorse Brand" adult eel feed produced by Fuzhou Seahorse Feed Co., Ltd.; the multivitamin is the shrimp multivitamin produced by Beijing Zhongnong Ranch Biotechnology Co., Ltd.; and the probiotics are the "Shrimp and Crab Guardian" mixed feed additive bacterial liquid produced by Nanjing Shenlaizhi Aquatic Technology Co., Ltd. The present invention prepares a suitable broodstock feed according to the growth characteristics of grayfin roughy, which helps to promote the maturation of the gonads of grayfin roughy and improve the quality of spawning and sperm production.

[0041] In the present invention, the luteinizing hormone releasing hormone in step S3 is LRH-A3, and the human chorionic gonadotropin HCG is purchased from Ningbo Sansheng Biotechnology Co., Ltd.

[0042] In the present invention, the injection dose of the male breeding broodstock in step S3 is half of the injection dose of the female breeding broodstock.

[0043] In the present invention, the injection site in step S3 is the dorsal fin or pectoral fin of the breeding parent fish.

[0044] In the present invention, in step S3, artificial spawning is performed on female broodstock whose gonads have developed to stage III-IV.

[0045] In the present invention, the environmental conditions for artificial induction of labor and fertilization in step S3 are water temperature 20.0-25.5°C, DO 6.89-9.77 mg / l, PH 7.791-8.022, and salinity 27.8-29.1.

[0046] In the present invention, the density of the fertilized eggs in step S4 is 150-200 eggs / L, the incubation conditions are water temperature 21.8°C, DO 7.58mg / l, pH 7.69, salinity 27.9, and the incubation time is 18-22h.

[0047] In the present invention, the grayfin seabream fry in step S4 have an average total length of 1.66 mm and a fertilized egg hatching rate of 93.5%.

[0048] In the present invention, step S5 includes the larvae-juvenile stage and the juvenile-young fish stage.

[0049] In the present invention, during the larvae-juvenile stage, unicellular algae are fed for static water cultivation, and aeration is increased by a Roots blower. The larvae density is 10,000 to 15,000 fish / m 3 , the density of unicellular algae is 100-200 / ml, the water temperature is maintained at 21.4-23.6°C, the salinity (SAL) is 27.9-29.4, the pH value is 7.706-7.901, and the dissolved oxygen (DO) is 6.79-7.26 mg / L during the cultivation stage. Ostreidae fertilized eggs and larvae are fed on the third day after hatching, followed by Brachionus sp., and an appropriate amount of Artemia (Brine shrimp) is fed every day after 10 days. Shrimp) or small copepods (Copepoda) and their nauplii, the content of the oyster fertilized eggs and their larvae is 2.0-4.0 pieces / ml, the density of the rotifer is 0.8-2.0 pieces / ml, and the density of the Artemia or small copepods and their nauplii is 0.05-1.5 pieces / ml; after 20 days, the fry and juvenile fish seedlings are fed with a compound feed, which is a SS-type fry and juvenile fish seedlings compound feed developed in Japan and produced in Weifang, Shandong. After 30 days, all the fry and juvenile fish are fed with the compound feed, 3-4 times a day, and the feeding amount is based on the feeding activity and satiety of the fry observed with the naked eye;

[0050] In the present invention, the juvenile fish are cultivated in a micro-flow water manner, and a Roots blower is used to increase oxygen. The juvenile fish density is 0.5-10,000 / m 3 , juvenile density 0.04~0.50 thousand / m 3 The water change rate is 10-20% per day, the water temperature during the cultivation period is 22.7-24.6℃, the salinity (SAL) is 28.5-29.3, the pH value is 7.706-7.901, and the dissolved oxygen content (DO) is 6.50-7.13mg / L. The fish are fed with S-LL1 (floating) marine fish compound feed produced in Weifang, Shandong, 3-4 times a day, and the feeding amount is 30-50g / 10,000 fish.

[0051] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention.

[0052] The experimental methods or test methods described in the following examples are conventional methods unless otherwise specified; the raw materials and auxiliary agents are obtained from conventional commercial channels or prepared by conventional methods unless otherwise specified.

[0053] Example 1

[0054] S1. Selection of broodstock:

[0055] From February to March, 106 wild grey-finned seabream were transported to the indoor taming pool of the Fujian Aquatic Seed Research Center in Zhangzhou Development Zone, Fujian Province. During the taming period, the whole pool was sprayed with 0.2ppm concentrated glutaraldehyde solution (for aquaculture) once every 3 days for 3 times to prevent and treat bacterial diseases caused by epidermal damage. After that, the drug was used once every 15 days. During the taming period, the water was aerated and the water was aerated after 24 hours. After the state stabilized, the healthy individuals with no external injuries on the body surface and normal body shape without disabilities were selected as broodstock for backup;

[0056] S2. Nutritional enhancement of broodstock:

[0057] After being fed with live seawater or freshwater shrimp for 12 days, the fish are fed with iced fish or shellfish for 15 days, and finally are fed with homemade feed for nutritional fortification. The homemade feed is a formula of adding 62.18% of fish paste, 37.62% of eel powder, 0.1% of multivitamins and 0.1% of probiotics. The feed is fed twice a day, and the daily feeding amount is 3.0% of the fish body weight. Under the conditions of water temperature of 22.4-25.1°C, pH 7.71-7.91, salinity of 29.1, and DO 6.89-8.12 mg / l, after 2 months of artificial domestication and intensive cultivation, 69 fish survive, with a survival rate of 65.09%; 20 female fish with mature gonads and 20 male fish are used as breeding broodstock, and the gonad maturity rate reaches 57.97%;

[0058] S3. Artificial insemination:

[0059] Artificial induction of female broodstock whose gonads have developed to stage III to IV is carried out by using a combination of luteinizing hormone-releasing hormone LRH-A3 and human chorionic gonadotropin HCG (produced by Ningbo Sansheng Biotechnology Co., Ltd., with a dose of 8.0 μg / kg+700 IU / kg calculated based on the body weight of the broodstock). The dose for male broodstock is halved, with a male-to-female ratio of 1:1. Each batch of broodstock is injected with hormones twice in the dorsal fin or pectoral fin, with an interval of 24 hours each time. Under the conditions of water temperature of 20.0-25.5°C, DO of 6.89-9.77 mg / l, pH of 7.791-8.022, and salinity of 27.8-29.1, the broodstock can achieve natural spawning and fertilization, and obtain fertilized eggs with a fertilization rate of 84.2%;

[0060] S4. Artificial incubation

[0061] 800,000 fertilized eggs were placed in a conical glass fiber reinforced plastic hatching bucket (specification 800x 800) for aquaculture for artificial aeration hatching, the fertilized egg density was 150-200 eggs / L, under the conditions of water temperature 21.8°C, DO7.58mg / l, pH7.69, and salinity 27.9, 748,000 fry were hatched after 18-22 hours, the average total length of the newly hatched fry was 1.66mm, and the fertilized egg hatching rate was 93.5%;

[0062] S5. Seedling cultivation:

[0063] 748,000 larvae of the grayfin seabream were transferred to 52m 2 In an indoor cement culture pond with a water depth of 1.4 m, feeding began on the third day after hatching until gray-finned seabream fry were obtained;

[0064] During the larvae-juvenile stage, the fish were cultured in a pond rich in unicellular algae and aerated by Roots blowers. The larvae density was 10,270 / m 3, the density of unicellular algae is 100-200 / ml; the water temperature during the cultivation period is 21.4-23.6℃, ​​the salinity (SAL) is 27.9-29.4, the pH value is 7.706-7.901, and the dissolved oxygen (DO) is 6.79-7.26mg / L; on the third day after hatching, oyster fertilized eggs and their larvae are fed, followed by Brachionus rotifers, and after 10 days, an appropriate amount of Artemia or small copepods and their nauplii are fed every day. Generally, oysters are often kept in the water. The density of oyster fertilized eggs and their larvae is 2.0-4.0 / ml, the density of rotifers is 0.8-2.0 / ml, and the density of Artemia or small copepods and their nauplii is 0.05-1.5 / ml. After 20 days, a small amount of SS-type (larvae and juveniles breeding) compound feed developed in Japan and produced in Weifang, Shandong Province is used for domestication. After 30 days, all larvae and juveniles are fed with compound feed, 4 times a day. The feeding amount is based on the feeding activity and satiety of the juveniles observed with the naked eye.

[0065] The juvenile-juvenile stage is cultivated in micro-flow water, and aeration is provided by Roots blowers. The juvenile density is 5,000 to 10,000 fish / m 3 , juvenile density 0.04~0.50 thousand / m 3 , the water change rate is 10-20% per day, the water temperature during the cultivation period is 22.7-24.6℃, the salinity (SAL) is 28.5-29.3, the pH value is 7.706-7.901, the dissolved oxygen (DO) is 6.50-7.13mg / L, and the fish are fed with S-LL1 type (floating) marine fish compound feed (larvae and fry breeding) produced in Weifang, Shandong Province, three times a day, and the feeding amount is 35g / 10,000 fish;

[0066] Through the above steps, after 60 days of cultivation, a total of 76,200 fry with an average total length of 3.51 cm and an average weight of 0.80 g were obtained, with a survival rate of 10.19%.

[0067] Comparative Example 1

[0068] The difference from Example 1 is that no nutritional enrichment cultivation of broodstock was carried out, and the results showed that there were no gonadal mature broodstock by the breeding season.

[0069] Comparative Example 2

[0070] The difference from Example 1 is that the density of fertilized eggs in step S4 is 29.0 eggs / L. The results show that the hatching rate of fertilized eggs is 49.0%.

[0071] Comparative Example 3

[0072] The difference from Example 1 is that in the larvae-juvenile stage of step S5, the pH value is 8.022-9.169. The results show that after 60 days of cultivation, the survival rate of the fry is 4.85%.

[0073] Comparative Example 4

[0074] The difference from Example 1 is that in the juvenile-young fish stage of step S5, the water temperature during the incubation period is 24.9-27.4° C. The results show that after 60 days of incubation, the survival rate of the young fish is 6.73%.

[0075] Comparative Example 5

[0076] The difference from Example 1 is that in the larvae-juvenile stage of step S5, the salinity (SAL) is 19.7-23.8. The results show that after 60 days of cultivation, the survival rate of the fry is 4.73%.

[0077] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for artificial breeding of grayfin porgy, characterized in that: The following steps are involved: S1. Selection of broodstock: The wild grey-finned seabream is transported to the domestication pond, and healthy individuals with no external injuries on the body surface, normal body shape and no disabilities are selected as broodstock for future use; S2. Nutritional enhancement of broodstock: After being fed with live seawater or freshwater shrimp for 10-15 days, the fish are fed with fresh ice fish or shellfish for 10-15 days, and finally transitioned to being fed with homemade feed for nutritional fortification. The homemade feed is a formula with added fish paste, eel meal, multivitamins and probiotics. The daily feeding amount is 3.0-5.0% of the fish body weight, and the fish are fed 1-2 times a day. Wild grayfin seabream are fed until the gonads mature, and the fish are used as breeding broodstock for standby use; S3. Artificial insemination: Injecting oxytocin luteinizing hormone releasing hormone and chorionic gonadotropin into the breeding broodstock in step S2 for artificial induction of labor, wherein the injection dose of the luteinizing hormone releasing hormone is 5.0-10.0 μg / kg, the injection dose of the chorionic gonadotropin is 300-1000 IU / kg, the male-female ratio is 1:1, and each batch of breeding broodstock is injected 3-5 times, each time interval is 24-36 hours, and the breeding broodstock naturally spawns and fertilizes to obtain fertilized eggs; S4. Artificial incubation: The fertilized eggs in step S3 are placed in an incubation bucket for artificial incubation until hatching, thereby obtaining gray-finned seabream fry; S5. Seedling cultivation: The grayfin thorny seabream fry described in step S4 is transferred to a cultivation pond, and food is started to be fed after the third day of hatching until grayfin thorny seabream fry is obtained.

2. The artificial breeding method of grayfin porgy according to claim 1, characterized in that: Step S1: In the early stage of domestication, the domestication pool is disinfected with a disinfectant, once every 2-3 days, 2-3 times in a row, and then once every 15 days. During the disinfection period, the water is purified and aerated, and the domestication is carried out with running water and aeration for 24 hours.

3. The artificial breeding method of grayfin porgy according to claim 1, characterized in that: The injection dose of the male breeding broodstock in step S3 is half of the injection dose of the female breeding broodstock.

4. The artificial breeding method of grayfin porgy according to claim 1, characterized in that: The injection site in step S3 is the dorsal fin or pectoral fin of the breeding parent fish.

5. The artificial breeding method of grayfin porgy according to claim 1, characterized in that: The environmental conditions for artificial induction of labor and fertilization in step S3 are water temperature 20.0-25.5° C., DO 6.89-9.77 mg / l, pH 7.791-8.022, and salinity 27.8-29.

1.

6. The artificial breeding method of grayfin porgy according to claim 1, characterized in that: The density of the fertilized eggs in step S4 is 150-200 eggs / L, the incubation conditions are water temperature 21.8°C, DO 7.58mg / l, pH 7.69, salinity 27.9, and the incubation time is 18-22h.

7. The artificial breeding method of grayfin roughy according to claim 1, characterized in that: The grayfin seabream fry described in step S4 have an average total length of 1.66 mm and a fertilized egg hatching rate of 93.5%.

8. The artificial breeding method of grayfin porgy according to claim 1, characterized in that: Step S5 includes the larvae-juvenile stage and the juvenile-young fish stage.

9. The artificial breeding method of grayfin roughy according to claim 1, characterized in that: During the larvae-juvenile stage, the fish are fed with unicellular algae for static water cultivation and oxygenation. The larvae density is 10,000 to 15,000 fish / m 3 , the density of unicellular algae is 100-200 / ml, the water temperature is maintained at 21.4-23.6°C, the salinity is 27.9-29.4, the pH value is 7.706-7.901, and the dissolved oxygen content is 6.79-7.26mg / L during the cultivation stage, and the fertilized oyster eggs and larvae thereof are fed on the third day after hatching, followed by the Brachionus rotifer, and after 10 days, an appropriate amount of Artemia or small copepods and nauplii thereof are fed every day, wherein the content of the fertilized oyster eggs and larvae thereof is 2.0-4.0 / ml, the density of the rotifer is 0.8-2.0 / ml, and the density of the Artemia or small copepods and nauplii thereof is 0.05-1.5 / ml; after 20 days, the fry and juvenile fish seedling formula feed is fed, and after 30 days, all the fry and juvenile fish are fed with the fry and juvenile formula feed, 3-4 times a day, and the feeding amount is based on the feeding activity and satiety state of the fry observed with the naked eye.

10. The artificial breeding method of grayfin roughy according to claim 1, characterized in that: The juvenile-juvenile stage is cultivated in a micro-flow water manner and oxygenated at the same time. The juvenile density is 0.5-10,000 fish / m 3 , juvenile density 0.04~0.50 thousand / m 3 The water change rate is 10-20% per day, the water temperature during the cultivation period is 22.7-24.6℃, the salinity is 28.5-29.3, the pH value is 7.706-7.901, the dissolved oxygen content is 6.50-7.13mg / L, and the fish are fed with marine fish compound feed, 3-4 times a day, and the feeding amount is 30-50g / 10,000 fish.

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