Device and method suitable for southern orius minutus breeding and predation function reaction determination

Abstract

The invention belongs to the field of insect feeding and collection, and particularly relates to a device and method suitable for southern orius minutus feeding and predation function reaction determination.The device comprises an egg hatching chamber, a nymph feeding chamber and an adult feeding chamber which are connected through a first plastic channel and a second plastic channel respectively; the first plastic channel and the second plastic channel are each provided with a valve, a first filter screen is arranged in the first plastic channel, and a second filter screen is arranged in the second plastic channel. The device is simple to operate, convenient to observe, convenient to replace preys, convenient to collect and capable of reducing inter-species disability and measuring the predation and parasitism functions of insects.

Description

Technical Field

[0001] The invention belongs to the field of insect breeding and collection, and in particular relates to a device and method suitable for breeding southern flower stink bugs and measuring predation function responses. Background Art

[0002] In the prevention and control of small pests such as western flower thrips, whiteflies and aphids, environmentally friendly prevention and control methods have always been the methods generally sought by researchers. Among them, natural enemy biological control is considered to be a more promising prevention and control measure. The natural enemy insects of the above-mentioned small pests can be divided into two categories: parasitic natural enemies and predatory natural enemies. Among them, the southern flower stink bug Orius similis belongs to the family of Hemiptera, and is an important predatory natural enemy insect. It has a long period of occurrence in the field, a large amount of prey, and can prey on a large number of pests. It has a good control effect on many small pests such as western flower thrips, aphids and whiteflies. It is a type of dominant natural enemy insect widely distributed in the southern ecosystem of my country.

[0003] At present, the main devices for individually raising and measuring the functional responses of natural enemies such as southern flower stink bugs are transparent plastic jars, plastic lunch boxes, culture dishes, etc., but due to the intraspecific cannibalism of predatory natural enemies such as southern flower stink bugs, it is difficult to raise them in large numbers in the same relatively small space during experiments. In traditional experimental devices or instruments, frequently adding water to the leaves to maintain freshness can easily cause the adult insects to escape or die, and this process disturbs them greatly.

[0004] Therefore, how to provide a device suitable for the feeding and predation functional response measurement of southern flower stink bugs and its use method has become an issue that technicians in this field urgently need to consider. Summary of the invention

[0005] In view of the problems existing in the above-mentioned prior art, the present invention discloses a device and a method for use suitable for the breeding and predation functional response measurement of southern flower stink bugs. The device of the present invention is simple to operate, convenient for observation, convenient for changing prey, convenient for collection, and reduces intraspecific cannibalism, and specifically discloses the following technical solutions:

[0006] A device suitable for rearing southern flower stink bugs and measuring their predation function response comprises an egg hatching chamber, a nymph rearing chamber and an adult rearing chamber, wherein the egg hatching chamber, the nymph rearing chamber and the adult rearing chamber are respectively connected via a first plastic channel and a second plastic channel, wherein the first plastic channel and the second plastic channel are both provided with valves, a first filter screen is provided in the first plastic channel, and a second filter screen is provided in the second plastic channel.

[0007] Furthermore, the egg hatching chamber includes a first plastic cover with gauze, a first plastic tank body and a first leaf "L"-shaped preservation tube, wherein the first plastic cover with gauze is threadedly connected to the first plastic tank body, and the first leaf "L"-shaped preservation tube is arranged on one side of the first plastic tank body and is connected to the first plastic tank body.

[0008] Furthermore, the nymph breeding chamber includes a second plastic cover with gauze, a second plastic tank body, a second leaf "L"-shaped preservation tube, a first cotton ball and a first syringe needle, the second plastic cover with gauze is threadedly connected to the second plastic tank body, the second leaf "L"-shaped preservation tube is arranged on one side of the second plastic tank body and is connected to the second plastic tank body, the first cotton ball is arranged inside the second plastic tank body, the first syringe needle is arranged on the tank wall of the second plastic tank body, and the needle tip of the first syringe needle is located on the surface of the first cotton ball.

[0009] Furthermore, the adult breeding chamber includes a third plastic cover with gauze, a third plastic tank body, a third leaf "L"-shaped preservation tube, a second syringe needle and a second cotton ball, the third plastic cover with gauze is threadedly connected to the third plastic tank body, the third leaf "L"-shaped preservation tube is arranged on one side of the third plastic tank body and is connected to the third plastic tank body, the second cotton ball is arranged inside the third plastic tank body, the second syringe needle is arranged on the tank wall of the third plastic tank body, and the needle tip of the second syringe needle is located on the surface of the second cotton ball.

[0010] Furthermore, the first plastic channel and the second plastic channel are both grey translucent plastic channels, and the first plastic tank body, the second plastic tank body and the third plastic tank body are all transparent tank bodies.

[0011] Furthermore, it also includes a vertical fresh-keeping tube, which is connected and arranged at the bottom of the first plastic tank body, the second plastic tank body and the third plastic tank body.

[0012] Furthermore, it also includes a plug, which is detachably plugged into the tube openings of the first leaf "L"-shaped fresh-keeping tube, the second leaf "L"-shaped fresh-keeping tube and the third leaf "L"-shaped fresh-keeping tube.

[0013] A method for measuring the functional response of feeding and predation of southern flower stink bugs is implemented by using the above-mentioned device for measuring the functional response of feeding and predation of southern flower stink bugs, and comprises the following steps:

[0014] S01. The entire device is placed in a suitable temperature and humidity environment, beans are placed in the adult breeding room and the forsythia branches are inserted into the third leaf "L" type preservation tube to provide a place for the southern flower bugs to lay eggs; bean leaves with whitefly nymphs are inserted into the third leaf "L" type preservation tube to provide food for the southern flower bugs, honey water is drawn by a syringe through the needle of the second syringe and injected into the second cotton ball to provide spare food for the southern flower bugs and the device is moisturized, and preparations for adult breeding are made;

[0015] S02. Place the southern flower bugs within 24 hours of initial emergence into the adult rearing room and observe them every 24 hours. When they lay eggs on beans and winter jasmine branches, remove them and transfer them to the egg incubation room;

[0016] S03. After observing that the eggs are about to hatch or just hatched, open the first valve, insert a large number of bean leaves carrying whitefly nymphs into the second leaf "L"-shaped preservation tube to provide food and provide a hiding place for southern flower stink bugs to reduce the mutual cannibalism of nymphs, use a syringe to draw honey water through the needle of the first syringe to inject the honey water into the first cotton ball to provide spare food and device moisture for southern flower stink bugs, and spray jasmonic acid on it to attract southern flower stink bugs nymphs to come for food, so as to reduce the mutual cannibalism of eggs;

[0017] S04. When the nymphs are observed to be about to emerge, open the valve on the second plastic channel and add bean leaves, jasmine flowers and beans with whiteflies to the adult rearing room to complete the entire rearing cycle.

[0018] Furthermore, when it is necessary to raise and observe the southern flower stink bug individually, only one egg needs to be placed in the egg incubation chamber and the operation steps S01-S04 are performed.

[0019] Furthermore, when it is necessary to measure the predation functional response of the southern flower stink bug, it is only necessary to put a fixed number of southern flower stink bug nymphs or adults into the corresponding breeding room, close the valves on both sides, and provide a predetermined number of pests, and follow the operating steps S01-S04 to complete the measurement of the predation functional response.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] Compared with transparent plastic jars and culture dishes, the present invention is more suitable for individual and small-batch experimental breeding of southern flower bugs at different developmental stages, reduces intraspecific cannibalism, and requires shorter operation time and more convenient operation during the breeding of southern flower bugs, making it easier to observe and collect and replace prey, and easier to complete the test requirements. The device of the present invention can complete the determination of the predation functional response of southern flower bugs. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the overall result when the present invention is in a feeding state;

[0023] Figure 2 This is a schematic diagram of the internal structure of the incubation chamber;

[0024] Figure 3 It is a structural schematic diagram of the nymph rearing room of the present invention;

[0025] Figure 4 This is a schematic diagram of the structure of the adult insect breeding room of the present invention;

[0026] Figure 5 The bar graph is a graph showing the effect of conventional operation on adults of Siberian stink bugs in Example 3;

[0027] Figure 6 The graph is a functional response curve of the predation of Similis minutus on Bemisia tabaci in different devices in Example 3;

[0028] Figure 7 This is a curve diagram of the searching effect of southern flower stink bug on whitefly in different devices in Example 3.

[0029] Among them, 1. Egg hatching room; 1.1. First plastic cover with gauze; 1.2. First plastic tank body; 1.3. First leaf "L" shaped fresh-keeping tube; 2. Nymph rearing room; 2.1. Second plastic cover with gauze; 2.2. Second plastic tank body; 2.3. Second leaf "L" shaped fresh-keeping tube; 2.4. First cotton ball; 2.5. First syringe needle; 2.6. Hole; 3. Adult rearing room; 3.1. Third plastic cover with gauze; 3.2. Plastic tank body; 3.3. Third leaf "L" shaped fresh-keeping tube; 3.4. Second syringe needle; 3.5. Second cotton ball; 4. First plastic channel; 5. Second plastic channel; 6. Valve; 7. Vertical fresh-keeping tube. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] Example 1

[0032] See attached Figure 1-4, the present embodiment discloses a device suitable for the breeding of southern flower stink bugs and the determination of predation function response, comprising: an egg hatching chamber 1, a nymph breeding chamber 2 and an adult breeding chamber 3, the egg hatching chamber 1, the nymph breeding chamber 2 and the adult breeding chamber 3 are respectively connected by a first plastic channel 4 and a second plastic channel 5, the first plastic channel 4 and the second plastic channel 5 are both provided with a valve 6, a first filter screen 4.1 is provided in the first plastic channel 4, and a second filter screen 5.1 is provided in the second plastic channel. In the present embodiment, the three breeding chambers have the same size, each breeding chamber has a diameter of 10 cm and a height of 5 cm, the first plastic channel 4 and the second plastic channel 5 have a diameter of 1.5 cm and a length of 3.5 cm, the first plastic channel 4 and the second plastic channel 5 and the valve 6 thereon are used to control the passage of southern flower stink bugs, and prevent the old southern flower stink bugs from killing each other, and the first filter screen 4.1 and the second filter screen 5.1 are used for screening, and the eggs, nymphs and adults are separated by setting the first filter screen 4.1 and the second filter screen 5.1 of different calibers.

[0033] The egg hatching chamber 1 comprises a first plastic cover with a gauze mesh 1.1, a first plastic tank body 1.2 and a first leaf "L"-shaped preservation tube 1.3. The first plastic cover with a gauze mesh 1.1 is threadedly connected to the first plastic tank body 1.2. The first leaf "L"-shaped preservation tube 1.3 is arranged on one side of the first plastic tank body 1.2 and is connected to the first plastic tank body 1.2. Specifically, a hole 2.6 is arranged on the connection side of the first plastic tank body 1.2. The first leaf "L"-shaped preservation tube 1.3 and the first plastic channel 4 are fixed to the first plastic tank body 1.2 by hot melt adhesive.

[0034] The nymph breeding chamber 2 comprises a second plastic cover with gauze 2.1, a second plastic tank body 2.2, a second leaf "L"-shaped fresh-keeping tube 2.3, a first cotton ball 2.4 and a first syringe needle 2.5. The second plastic cover with gauze 2.1 is threadedly connected to the second plastic tank body 2.2, the second leaf "L"-shaped fresh-keeping tube 2.3 is arranged on one side of the second plastic tank body 2.2 and is connected to the second plastic tank body 2.2, the first cotton ball 2.4 is arranged inside the second plastic tank body 2.2, the first syringe needle 2.5 is arranged on the tank wall of the second plastic tank body 2.2, the needle tip of the first syringe needle 2.5 is located on the surface of the first cotton ball 2.4, the first syringe needle 2.5 is used to add honey water to the first cotton ball 2.4, and the honey water cotton ball is used to provide a partial food source for the nymphs and maintain humidity for the device.

[0035] The adult breeding chamber 3 comprises a third plastic cover with gauze 3.1, a third plastic tank body 3.2, a third leaf "L"-shaped preservation tube 3.3, a second syringe needle 3.4 and a second cotton ball 3.5. The third plastic cover with gauze 3.1 is threadedly connected to the third plastic tank body 3.2, the third leaf "L"-shaped preservation tube 3.3 is arranged on one side of the third plastic tank body 3.2 and is connected to the third plastic tank body 3.2, the second cotton ball 3.5 is arranged inside the third plastic tank body 3.2, the second syringe needle 3.4 is arranged on the tank wall of the third plastic tank body 3.2, and the needle tip of the second syringe needle 3.4 is located on the surface of the second cotton ball 3.5.

[0036] The first plastic channel 4 and the second plastic channel 5 are both grey translucent plastic channels, and the first plastic tank body 1.2, the second plastic tank body 2.2 and the third plastic tank body 3.2 are all transparent tank bodies.

[0037] As a preferred embodiment of the present invention, it also includes a vertical fresh-keeping tube 7, which is connected and arranged at the bottom of the first plastic tank body 1.2, the second plastic tank body 2.2 and the third plastic tank body 3.2.

[0038] As a preferred embodiment of the present invention, it also includes a plugging device that is detachably plugged into the tube openings of the first leaf "L"-shaped preservation tube 1.3, the second leaf "L"-shaped preservation tube 2.3 and the third leaf "L"-shaped preservation tube 3.3 to prevent southern small flower stink bugs from escaping from the "L"-shaped preservation tubes.

[0039] Example 2

[0040] This embodiment discloses a determination method using the determination device in Embodiment 1, comprising the following steps:

[0041] S01. The entire device is placed in a suitable temperature and humidity environment, and beans are placed in the adult breeding room 3 and the forsythia branches are inserted into the third leaf "L" type preservation tube 3.3 to provide a place for the laying of eggs by the adults of southern flower bugs; the bean leaves with whitefly nymphs are inserted into the third leaf "L" type preservation tube 3.3 to provide food for the adults of southern flower bugs, and honey water is drawn by a syringe through the second syringe needle 3.4 to inject honey water into the second cotton ball 3.5 to provide spare food and device moisture for southern flower bugs, and prepare for adult breeding;

[0042] S02. The southern flower stink bugs within 24 hours of initial emergence were placed in the adult breeding room 3 and observed every 24 hours. After laying eggs in beans and winter jasmine branches, the two were removed and transferred to the egg incubation room 1;

[0043] S03. After observing that the eggs are about to hatch or just hatched, open the first valve 6, insert a large number of bean leaves with whitefly nymphs into the second leaf "L" type preservation tube 2.3 to provide food and provide a place for southern flower stink bugs to avoid, reduce the mutual cannibalism of nymphs, use a syringe to draw honey water through the first syringe needle 2.5 to inject honey water into the first cotton ball 2.4 to provide spare food and device moisture for southern flower stink bugs, and spray jasmonic acid therein to attract southern flower stink bugs nymphs to come foraging, so as to reduce the mutual cannibalism of eggs;

[0044] S04. When the nymphs are observed to be about to emerge, the valve 6 on the second plastic channel 5 is opened, and bean leaves with whiteflies, winter jasmine and beans are added to the adult rearing chamber 3, thereby completing the entire rearing cycle.

[0045] When it is necessary to raise and observe the southern flower stink bug individually, only one egg needs to be placed in the egg hatching chamber 1 and the above operation is performed.

[0046] When it is necessary to measure the predation functional response of southern flower bugs, it is only necessary to put a fixed number of southern flower bug nymphs or adults into the corresponding breeding room, close the valves on both sides, and provide a certain number of pests to complete the measurement of the predation functional response according to the above operation.

[0047] Example 3

[0048] This embodiment and the conventional test device for Siberian stink bugs were used to conduct a comparative test on feeding and predation function response. The test was conducted in a controllable constant temperature box under the same conditions, using the device of the present invention and transparent plastic jars, culture dishes and other devices to compare the experimental operation time, completion degree, insect survival rate and other indicators.

[0049] Feeding test: Feeding starts from eggs, taking 100 eggs of Siberian small flower stink bug within 24 hours of first laying per device, repeating 3 groups, the egg size and development degree are kept consistent, and feeding is carried out according to the operating steps in the present invention, transparent plastic jars, and culture dishes, respectively, using the same management method, and the time required for the operation and the disturbance are counted, and the data such as the death number of nymphs of Siberian small flower stink bug, the escape rate of adults, and the development time are observed and counted.

[0050] Predation functional response: 3rd instar whitefly nymphs (5, 10, 15, 20, 25, 30 heads / box) were placed in each feeding room of this device, and 1 southern flower stink bug adult was placed in the corresponding adult feeding room, and the rest of the devices were operated in the same way. After 24 hours, the predation amount of southern flower stink bug adults and nymphs on 3rd instar whitefly nymphs at each density was observed and counted, and each treatment was repeated 3 times. The predation functional response data of southern flower stink bug were processed by Microsoft Excel 2019 and fitted by Origin2024b to obtain the model of dynamic relationship in the predator-prey system.

[0051] If P1 = 0, the parasitism of the parasitoid increases linearly with the increase of prey density, which belongs to Holling I type;

[0052] If P1 < 0, and the parasite's parasitism increases with the increase of prey density and then remains at a stable state, it belongs to Holling II type;

[0053] If P1>0, and the amount of parasitism by the parasite fluctuates in an "S" shape with the prey density, it belongs to Holling type III.

[0054] (1) Experiment on rearing southern flower stink bug

[0055] Table 1 The time required for raising southern flower bug eggs in different devices

[0056]

[0057] Note: The data in the table are mean ± standard error. The same lowercase letters indicate no significant difference, and different lowercase letters indicate significant difference. The significance of the difference was calculated using SPSS27.

[0058] The operation time required for raising southern flower stink bugs using different breeding devices is shown in Table 1.

[0059] Under the same conditions, when 100 southern flower stink bug eggs were raised at the same time, the time required for the device of the present invention to complete leaf access, leaf watering, egg access, and complete the entire operation was significantly short, which were 3.17 min, 0.83 min, 2.53 min, and 7.43 min, respectively (leaf access: F 2,8 =1137.00, P<0.001; leaf water addition: F 2,8 =94.9, P<0.001; egg access: F 2,8 =271.662, P<0.001; Total time to complete: F 2,8 =157.494, P<0.001; meanwhile, the device has a significantly higher leaf water retention time than the other two commonly used devices, which can last for 5.15 days (F 2,8 =2923.69, P<0.001).

[0060] Effects of routine operations on adults of southern flower bugs

[0061] See also Figure 5 When replacing the leaves, the culture dish disturbed the southern flower bug the most, and the present invention disturbed the southern flower bug the least (1.33 times). 2,8=96.257, P<0.001). There were significant differences in the adult escape rates caused by replacing plants (leaves) among the three devices. The lowest was 0.67% for the device of the present invention, followed by 6.33% for the transparent plastic jar, and the highest was 15.33% for the culture dish (F 3,11 =54.704, P<0.001).

[0062] (2) Functional response of southern flower bug

[0063] Table 2 Functional response parameters of southern flower bug adults in different devices

[0064]

[0065] From Table 1, it can be seen that when the three devices were used to determine the functional response model of the southern flower stink bug, the P1 values ​​were all less than 1, and all belonged to the Holling II type. Figure 6 It can be seen that as the density of whitefly nymphs increases, the predation ability of southern flower bugs gradually increases and tends to stabilize when the density reaches about 15 nymphs. Figure 7 In the experiment, the searching effect gradually decreased with the increase of the density of whitefly nymphs, and the predation amount, functional response model and searching effect of southern flower stink bug under the three devices showed very similar trends.

[0066] (I) Analysis of the results of rearing southern flower stink bugs:

[0067] The results of the southern flower bug rearing test show that the device of the present invention is significantly superior to the other two traditional insect rearing devices in rearing southern flower bugs. The device and method of the present invention are suitable for individual and small batch experimental rearing of southern flower bugs at different developmental stages. It can be seen from the rearing test that the device of the present invention is more convenient to operate, more time-saving, and is not easy to cause adult insects to escape during rearing. Its transparent gauze and transparent acrylic plate structure are also conducive to observation.

[0068] When raised in transparent plastic tanks, southern flower stink bugs are prone to intraspecific cannibalism due to their small size and space, making them unsuitable for long-term breeding. They also need to be added with water in a centrifuge tube to keep the leaves fresh, and need to be regularly added with water in a short period of time, which increases the operation process. Similar reasons also exist for culture dishes, and the gaps in the culture dishes themselves are too large, making it very easy for adults to escape.

[0069] (II) Analysis of the results of the test on the predation function of southern flower stink bug

[0070] The test results of the predation function of southern flower bugs at different developmental stages show that the device of the present invention is similar to the transparent plastic tank in structure and the results are similar in this respect and can achieve the expected effect, indicating that the device of the present invention is competent for the predation function response test of southern flower bugs.

[0071] The present invention describes in detail the rearing, predation functional response and results of southern flower stink bugs. However, by using the method and structure of the present invention, with only slight adjustments, such as replacement of host plants, replacement of natural enemies, replacement of pests, size and shape of each compartment, etc., some pests such as cabbage loopers, diamondback moths, whiteflies and some natural enemy insects such as trichogrammatids, tomato leafminers, and tobacco blind bugs can be reared and their functional responses can be measured.

[0072] In summary, the device of the present invention requires shorter operation time in the process of raising southern flower stink bugs, is more convenient to operate, and is easier to complete the test requirements. The device of the present invention can complete the determination of the predation function response of southern flower stink bugs.

[0073] The above description is only a preferred embodiment of the present invention and does not limit the technical scope of the present invention. Therefore, any slight modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A device suitable for the breeding of Siberian stink bugs and the determination of predation functional response, characterized in that: include: An egg hatching chamber (1), a nymph rearing chamber (2) and an adult rearing chamber (3), wherein the egg hatching chamber (1), the nymph rearing chamber (2) and the adult rearing chamber (3) are respectively connected via a first plastic channel (4) and a second plastic channel (5), wherein the first plastic channel (4) and the second plastic channel (5) are both provided with a valve (6), a first filter screen (4.1) is provided in the first plastic channel (4), and a second filter screen (5.1) is provided in the second plastic channel.

2. A device suitable for the breeding and predation functional response determination of Siberian stink bug according to claim 1, characterized in that: The egg hatching chamber (1) comprises a first plastic cover with a gauze mesh (1.1), a first plastic tank body (1.2) and a first leaf "L"-shaped fresh-keeping tube (1.3); the first plastic cover with a gauze mesh (1.1) is threadedly connected to the first plastic tank body (1.2); the first leaf "L"-shaped fresh-keeping tube (1.3) is arranged on one side of the first plastic tank body (1.2) and is in communication with the first plastic tank body (1.2).

3. A device suitable for the breeding and predation functional response determination of Siberian stink bug according to claim 2, characterized in that: The nymph breeding chamber (2) comprises a second plastic cover with gauze (2.1), a second plastic tank body (2.2), a second leaf "L"-shaped fresh-keeping tube (2.3), a first cotton ball (2.4) and a first syringe needle (2.5); the second plastic cover with gauze (2.1) is threadedly connected to the second plastic tank body (2.2); the second leaf "L"-shaped fresh-keeping tube (2.3) is arranged on one side of the second plastic tank body (2.2) and is connected to the second plastic tank body (2.2); the first cotton ball (2.4) is arranged inside the second plastic tank body (2.2); the first syringe needle (2.5) is arranged on the tank wall of the second plastic tank body (2.2); and the needle tip of the first syringe needle (2.5) is located on the surface of the first cotton ball (2.4).

4. A device suitable for the breeding and predation functional response determination of Siberian stink bug according to claim 3, characterized in that: The adult insect breeding chamber (3) comprises a third plastic cover with a gauze mesh (3.1), a third plastic tank body (3.2), a third leaf "L"-shaped fresh-keeping tube (3.3), a second syringe needle (3.4) and a second cotton ball (3.5); the third plastic cover with a gauze mesh (3.1) is threadedly connected to the third plastic tank body (3.2); the third leaf "L"-shaped fresh-keeping tube (3.3) is arranged on one side of the third plastic tank body (3.2) and is connected to the third plastic tank body (3.2); the second cotton ball (3.5) is arranged inside the third plastic tank body (3.2); the second syringe needle (3.4) is arranged on the tank wall of the third plastic tank body (3.2); and the needle tip of the second syringe needle (3.4) is located on the surface of the second cotton ball (3.5).

5. A device suitable for the breeding and predation functional response determination of Siberian stink bug according to claim 4, characterized in that: The first plastic channel (4) and the second plastic channel (5) are both grey translucent plastic channels, and the first plastic tank body (1.2), the second plastic tank body (2.2) and the third plastic tank body (3.2) are all transparent tank bodies.

6. A device suitable for the breeding and predation functional response determination of Siberian stink bug according to claim 4, characterized in that: It also comprises a vertical fresh-keeping tube (7), which is arranged in communication at the bottom of the first plastic tank body (1.2), the second plastic tank body (2.2) and the third plastic tank body (3.2).

7. A device suitable for the breeding and predation functional response determination of Siberian stink bug according to claim 4, characterized in that: It also includes a plug which is detachably plugged into the tube openings of the first leaf "L"-shaped fresh-keeping tube (1.3), the second leaf "L"-shaped fresh-keeping tube (2.3) and the third leaf "L"-shaped fresh-keeping tube (3.3).

8. A method for measuring the functional response of feeding and predation of Siberian stink bugs, characterized in that: The method is implemented by using the device for feeding and measuring the predation function response of Siberian stink bugs as described in any one of claims 1 to 7, comprising the following steps: S01. The entire device is placed in a suitable temperature and humidity environment, and beans are placed in the adult breeding room (3) and the forsythia branches are inserted into the third leaf "L" type preservation tube (3.3) to provide a place for the southern flower bugs to lay eggs; the bean leaves with whitefly nymphs are inserted into the third leaf "L" type preservation tube (3.3) to provide food for the southern flower bugs, and honey water is drawn by a syringe through the second syringe needle (3.4) to inject the honey water into the second cotton ball (3.5) to provide spare food for the southern flower bugs and the device is moisturized, and the adult breeding is prepared; S02. Place the southern flower bug within 24 hours of initial emergence into the adult breeding room (3) and observe it every 24 hours. After laying eggs on beans and winter jasmine branches, remove the two and transfer them to the egg incubation room (1); S03. After observing that the eggs are about to hatch or just hatched, open the first valve (6), insert a large number of bean leaves carrying whitefly nymphs into the second leaf "L"-shaped preservation tube (2.3) to provide food and provide a hiding place for southern flower stink bugs to reduce the mutual cannibalism of nymphs, use a syringe to draw honey water through the first syringe needle (2.5) and inject the honey water into the first cotton ball (2.4) to provide spare food for southern flower stink bugs and moisturize the device, and spray jasmonic acid on it to attract southern flower stink bug nymphs to come for food, so as to reduce the mutual cannibalism of eggs; S04. When the nymphs are observed to be about to emerge, the valve (6) on the second plastic channel (5) is opened, and bean leaves, jasmine flowers and beans with whiteflies are added to the adult rearing room (3), thereby completing the entire rearing cycle.

9. A method for determining the functional response of Siberian stink bug breeding and predation according to claim 8, characterized in that: When it is necessary to raise and observe the southern flower stink bug individually, only one egg needs to be placed in the egg hatching chamber (1) and the operation steps S01-S04 are followed.

10. A method for determining the functional response of Siberian stink bug breeding and predation according to claim 8, characterized in that: When it is necessary to measure the predation functional response of southern flower stink bugs, it is only necessary to put a fixed number of southern flower stink bugs nymphs or adults into the corresponding breeding room, close the valves on both sides, and provide a predetermined number of pests, and follow the operating steps S01-S04 to complete the measurement of the predation functional response.

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