Sodium selenite composite liquid for increasing selenium content of soybeans and application of sodium selenite composite liquid

Abstract

The invention discloses a sodium selenite composite liquid for increasing the selenium content of soybeans and application thereof, relates to the field of soybeans, and aims to increase the selenium content, especially the organic selenium content, of Dongnong beans 358 so as to improve the cholesterol reduction capability of the Dongnong beans 358. The provided sodium selenite composite liquid is composed of a sodium selenite solution, arbuscular mycorrhizal fungi and a ganoderma lucidum sporocarp solution. Wherein the concentration of sodium selenite in the sodium selenite composite liquid is 30-100 mg / L, the total bacterial count of arbuscular mycorrhizal fungi is 1 * 10 < 8 >-1 * 10 < 10 > CFU / mL, and the concentration of the ganoderma lucidum sporocarp solution is 10-20 mg / L. The ganoderma lucidum sporocarp is added, so that the selenium enrichment amount of Dongnong bean 358 soybeans is remarkably increased, the conversion rate of organic selenium is improved, the selenium content of Dongnong bean 358 is increased, especially the content of organic selenium is increased, and the cholesterol lowering effect of Dongnong bean 358 is improved. The market value is improved.

Description

Technical Field

[0001] The invention belongs to the field of soybeans, and particularly relates to a sodium selenite composite liquid for increasing the selenium content of soybeans. Background Art

[0002] As an essential micronutrient for the human body with antioxidant properties, selenium has the functions of preventing cancer, enhancing the body's immunity, preventing and treating cardiovascular diseases, and delaying human aging. Organic selenium, especially protein selenium and amino acid selenium, has the advantages of high bioavailability, strong biological activity and high safety, and has become a hot topic in current research. Soybeans have a certain ability to enrich selenium, can actively absorb selenium in the environment and convert inorganic selenium into organic selenium. Soy peptides are a mixture obtained by separating and purifying soybean protein after protease hydrolysis. The amino acid absorption and transport rate of soybean peptides are higher than that of proteins and free amino acids containing the same amount of amino acids. It has functional characteristics such as regulating immune function, anti-oxidation, lowering blood pressure, lowering cholesterol, and anti-fatigue. Northeast Agricultural University, Dongnong Bean 358 has a large amount of cholesterol-lowering special protein enrichment, high arginine content, high protein, and low allergenicity. The cholesterol-lowering soybean protein powder 'Dongnong Bean 358-SPI' produced with 'Dongnong Bean 358' has the same effect as the cholesterol-lowering drug "fenofibrate" in reducing total cholesterol, triglycerides, and low-density lipoprotein levels in the blood, and increasing the high-density lipoprotein content, which can avoid the adverse side effects of "fenofibrate". 'Dongnong Bean 358-SPI' is currently the first cholesterol-lowering soybean protein powder in China and abroad that can achieve drug therapy effects through diet therapy. It has the characteristics of good protein universality and outstanding cholesterol-lowering effects. Current studies have shown that selenium can lower blood cholesterol levels and help prevent heart disease, atherosclerosis and other diseases. Fu Di et al. (1997) specifically studied the effects of selenium on blood lipids and fatty liver. The results showed that selenium supplementation in the case of high blood lipids can significantly reduce cholesterol, triglycerides and low-density lipoprotein levels, while significantly increasing high-density lipoprotein levels. Therefore, increasing the selenium content of Dongnong Bean 358 will help improve its cholesterol-lowering ability. Summary of the invention

[0003] The purpose of the present invention is to increase the selenium content of Dongnong Bean 358, especially the organic selenium content, and thus enhance its cholesterol-lowering ability, and provide a sodium selenite complex liquid for increasing the selenium content of soybeans.

[0004] The invention discloses a sodium selenite composite liquid for improving the selenium content of soybeans. The sodium selenite composite liquid is composed of a sodium selenite solution, arbuscular mycorrhizal fungi and a ganoderma lucidum fruiting body solution. The concentration of sodium selenite in the sodium selenite composite liquid is 30-100 mg / L, the total number of arbuscular mycorrhizal fungi is 1×10 8 ~1×10 10CFU / mL, the concentration of Ganoderma lucidum fruiting body solution is 10-20 mg / L.

[0005] Furthermore, the concentration of sodium selenite in the sodium selenite bacterial agent solution is 50-80 mg / L.

[0006] Furthermore, the concentration of the Ganoderma lucidum fruiting body solution is 15-20 mg / L.

[0007] Furthermore, the ganoderma lucidum fruiting body is passed through 80-100 meshes.

[0008] The invention discloses an application of a sodium selenite composite liquid for increasing the selenium content of soybeans. The sodium selenite composite liquid is used in the soybean seedling stage to increase the selenium content and the organic selenium content of the soybeans.

[0009] Furthermore, the sodium selenite bacterial agent composite liquid is used during the soybean seedling period, and the specific method of use is as follows:

[0010] The disinfected and cleaned Dongnongdou 358 soybean seeds are added to a sodium selenite complex solution containing a sodium selenite solution and a Ganoderma lucidum fruiting body solution, and soaked at a temperature of 20 to 36°C for 2 to 12 hours. The soaked seeds are then placed on a seedling tray, covered with soil, and the arbuscular mycorrhizal fungi in the sodium selenite complex solution are added, followed by cultivation at a temperature of 23 to 37°C for 96 to 120 hours.

[0011] Furthermore, the soaking is carried out at a temperature of 25 to 36° C. for 48 to 120 hours.

[0012] Furthermore, the disinfected and cleaned soybean seeds are added to a sodium selenite complex solution and soaked for 4 to 8 hours at a temperature of 25 to 36° C.; wherein the concentration of sodium selenite in the sodium selenite complex solution is 50 to 100 mg / L, and the concentration of the Ganoderma lucidum fruiting body solution is 15 to 20 mg / L.

[0013] The present invention has the following beneficial effects:

[0014] In the soybean seedling cultivation, the present invention significantly improves the selenium enrichment of Dongnongdou 358 soybean by adding Ganoderma lucidum fruiting bodies in the existing sodium selenite seed soaking process, and finds that it also has the ability to improve the conversion rate of organic selenium. The scheme of the present invention can make the conversion rate of organic selenium of Dongnongdou 358 soybean in seedling cultivation reach about 56%. After adding Ganoderma lucidum fruiting bodies, the present invention further optimizes the seed soaking process according to the characteristics of Dongnongdou 358, and obtains a process suitable for soaking seeds with Ganoderma lucidum fruiting bodies and sodium selenite, which further improves the ability of soybean to enrich selenium. In order to further improve the ability of soybean to enrich selenium, the present invention adds arbuscular mycorrhizal fungi in seedling cultivation, so that the selenium enrichment ability of soybean is significantly improved. The present invention increases the selenium content of Dongnongdou 358, especially the content of organic selenium, and improves its cholesterol-lowering effect. Improve its market value. DETAILED DESCRIPTION

[0015] In order to make the objectives, technical solutions and advantages of the embodiments of the present invention more clearly understood, the spirit of the contents disclosed by the present invention will be described in detail below. After understanding the embodiments of the contents of the present invention, any technician in the relevant technical field can change and modify them according to the techniques taught by the contents of the present invention without departing from the spirit and scope of the contents of the present invention.

[0016] The exemplary embodiments of the present invention and the description thereof are used to explain the present invention but are not intended to limit the present invention.

[0017] Example 1

[0018] The method for increasing the selenium content of soybeans in this embodiment is as follows:

[0019] The disinfected and cleaned Dongnongdou 358 soybean seeds were added to a sodium selenite complex solution containing a sodium selenite solution and a Ganoderma lucidum fruiting body solution, and soaked for 6 hours at a temperature of 35±1°C. The soaked seeds were then placed on a seedling tray, covered with soil, and then the arbuscular mycorrhizal fungi in the sodium selenite complex solution were added. The seeds were then cultured for 120 hours at a temperature of 25±1°C. The concentration of sodium selenite in the sodium selenite complex solution was 80 mg / L, and the total number of arbuscular mycorrhizal fungi was 1×10 8 ~1×10 10 CFU / mL, the concentration of the Ganoderma lucidum fruiting body solution is 15 mg / L, and the Ganoderma lucidum fruiting body is prepared into a solution with a concentration of 15 mg / L after passing through 80-100 meshes.

[0020] The soaking temperature, soaking time, sodium selenite concentration, Ganoderma lucidum fruiting body solution concentration, total selenium of total arbuscular mycorrhizal fungi count, and organic selenium conversion capacity after 120 hours of cultivation in this example are shown in Table 1. Among them, selenium was determined by hydride generation atomic fluorescence spectrometry. Organic selenium was determined by continuous dialysis, and organic selenium conversion rate (%) = organic selenium / total selenium × 100%.

[0021] Table 1

[0022]

[0023] As shown in Table 1, the enrichment of selenium in soybeans was significantly increased by adding ganoderma lucidum fruiting bodies in this embodiment. In addition, during the soaking, the soaking temperature, soaking time, concentration of sodium selenite, concentration of ganoderma lucidum fruiting body solution, and total number of arbuscular mycorrhizal fungi all had an effect on the enrichment of total selenium, while the conversion of organic selenium was affected by the concentration of sodium selenite and the concentration of ganoderma lucidum fruiting body solution. This embodiment found that the addition of ganoderma lucidum fruiting bodies and the absence of ganoderma lucidum fruiting bodies had a greater effect on the conversion of selenium into organic selenium in soybeans, and also played an important role in the enrichment of total selenium.

Claims

1. A sodium selenite composite liquid for increasing the selenium content of soybeans, characterized in that The sodium selenite composite solution is composed of a sodium selenite solution, arbuscular mycorrhizal fungi and a ganoderma lucidum fruiting body solution; wherein the concentration of sodium selenite in the sodium selenite composite solution is 30-100 mg / L, the total number of arbuscular mycorrhizal fungi is 1×10 8 ~1×10 10 CFU / mL, the concentration of Ganoderma lucidum fruiting body solution is 10-20 mg / L.

2. The sodium selenite composite solution for increasing the selenium content of soybeans according to claim 1, characterized in that The concentration of sodium selenite in the sodium selenite bacterial agent liquid is 50-80 mg / L.

3. The sodium selenite composite solution for increasing the selenium content of soybeans according to claim 1, characterized in that The concentration of the ganoderma lucidum fruiting body solution is 15-20 mg / L.

4. A sodium selenite composite solution for increasing the selenium content of soybeans according to claim 1 or 3, characterized in that The ganoderma lucidum fruiting body is 80-100 meshes.

5. The use of a sodium selenite composite liquid for increasing the selenium content of soybeans as claimed in claim 1, characterized in that The sodium selenite composite liquid is used during the soybean seedling raising period to increase the selenium content and organic selenium content of the soybean.

6. The use according to claim 5, characterized in that The sodium selenite bacterial agent composite liquid is used during the soybean seedling raising period, and the specific method of use is as follows: The disinfected and cleaned Dongnongdou 358 soybean seeds are added to a sodium selenite complex solution containing a sodium selenite solution and a Ganoderma lucidum fruiting body solution, and soaked at a temperature of 20 to 36°C for 2 to 12 hours. The soaked seeds are then placed on a seedling tray, covered with soil, and the arbuscular mycorrhizal fungi in the sodium selenite complex solution are added, followed by cultivation at a temperature of 23 to 37°C for 96 to 120 hours.

7. The use according to claim 6, characterized in that The soaking is carried out at a temperature of 25 to 36° C. for 48 to 120 hours.

8. The use according to claim 6, characterized in that The disinfected and cleaned soybean seeds are added into a sodium selenite complex solution and soaked for 4 to 8 hours at a temperature of 25 to 36° C., wherein the concentration of sodium selenite in the sodium selenite complex solution is 50 to 100 mg / L, and the concentration of the ganoderma lucidum fruiting body solution is 15 to 20 mg / L.

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