Aronia melanocarpa fruit product capable of improving sleep and resisting oxidation and preparation method of aronia melanocarpa fruit product

Abstract

The invention belongs to the field of functional foods or health foods, and particularly relates to an aronia melanocarpa fruit product capable of improving sleep and resisting oxidation and a preparation method of the aronia melanocarpa fruit product. The aronia melanocarpa fruit beverage is prepared from the following components in parts by weight: 20 to 30 parts of aronia melanocarpa fruit juice, 0.5 to 2 parts of gamma-aminobutyric acid, 0.5 to 2 parts of tea leaf theanine, 0.5 to 2 parts of rhizoma gastrodiae, 0.1 to 1 part of semen myristicae, 0.1 to 1 part of fructus mume, 0.05 to 0.15 part of passion flower extract and 0.05 to 0.15 part of saffron crocus extract. Wherein the aronia melanocarpa fruits are used as a main raw material, other medicinal and edible raw materials are used as auxiliary materials, and the product can be taken daily, is used for improving symptoms such as insomnia and the like and has a very good anti-oxidation effect.

Description

Technical Field

[0001] The invention belongs to the field of functional foods or health foods, and particularly relates to an aronia nigra fruit product having the functions of improving sleep and resisting oxidation and a preparation method thereof. Background Art

[0002] Sleep is a necessary process for human physiological activities, which is controlled by the biological clock, the sleep-wake balance mechanism in the body, and will and behavior. Insomnia refers to a sleep disorder characterized by frequent and persistent difficulty falling asleep or maintaining sleep, accompanied by fatigue, attention deficit, and emotional instability, under the influence of factors such as genetic variation, life stress, major life events, and abnormal brain structure and function. With the accelerated pace of life in modern society, the increasing pressure of survival and the continuous changes in lifestyle, human sleep is under serious threat, and the incidence of insomnia is increasing year by year.

[0003] At present, the drugs for treating insomnia mainly include antidepressants, melatonin drugs, etc., but there are many adverse reactions such as dependence, tolerance, damage to cognitive function and withdrawal reactions. Therefore, it is necessary to develop a product that can safely and effectively calm the nerves and help sleep, and improve sleep quality, in order to meet the urgent needs of the vast number of people with insomnia and sleep disorders who hope to sleep well through diet. At the same time, if the product has antioxidant effects and can improve skin aging, it will have broad market prospects. Summary of the invention

[0004] To this end, the present invention provides a black fruit Aronia oleracea berry product with the function of improving sleep. The product uses black fruit Aronia oleracea berry as a main raw material, supplemented with other raw materials of medicinal and edible properties. The product can be taken daily to improve symptoms such as insomnia, and the product has a good antioxidant effect.

[0005] The technical contents of the present invention are:

[0006] A aronia nigra fruit product for improving sleep and resisting oxidation comprises, by weight, 20-30 parts of aronia nigra juice, 0.5-2 parts of gamma-aminobutyric acid, 0.5-2 parts of tea theanine, 0.5-2 parts of gastrodia elata, 0.1-1 parts of nutmeg, 0.1-1 parts of ebony, 0.05-0.15 parts of passionflower extract and 0.05-0.15 parts of saffron extract.

[0007] Preferably, the composition comprises, by weight, 22-28 parts of Aronia nigra juice, 0.7-1.2 parts of γ-aminobutyric acid, 0.8-1.2 parts of tea theanine, 0.8-1.2 parts of Gastrodia elata, 0.3-0.6 parts of nutmeg, 0.3-0.6 parts of ebony, 0.08-0.12 parts of passionflower extract and 0.1-0.13 parts of saffron extract.

[0008] Preferably, the weight ratio of the Aronia nigra juice, nutmeg and black plum is (22-28): (0.3-0.6): (0.3-0.6).

[0009] Further preferably, the weight ratio of the Aronia nigra juice, nutmeg and ebony is 25:0.5:0.5.

[0010] Preferably, the weight ratio of Aronia nigra juice, γ-aminobutyric acid, tea amino acids and Gastrodia elata is (22-28): (0.7-1.2): (0.8-1.2): (0.8-1.2).

[0011] Further preferably, the weight ratio of the Aronia nigromaculata juice, γ-aminobutyric acid, tea amino acids and Gastrodia elata is 25:1:1:1.

[0012] Preferably, the preparation of the above-mentioned Aronia nigra juice comprises the following steps: adding an enzyme preparation to Aronia nigra pulp, performing enzymolysis at 40-50°C for 3-4h, filtering out the pomace, and then inactivating the enzyme at 90°C for 5min to obtain Aronia nigra juice.

[0013] Preferably, the added amount of the enzyme preparation is 0.04-0.05%, and the enzyme preparation consists of cellulase and pectinase in a weight ratio of 5:4.

[0014] More preferably, the Aronia nigra fruits are selected, washed, stemmed and squeezed to obtain Aronia nigra fruit pulp.

[0015] More preferably, the enzymatic activity of the cellulase is 30,000 U / g, and the enzymatic activity of the pectinase is 50,000 U / g.

[0016] Preferably, fruit concentrated juice is also included, specifically, by weight, 12-25 parts of peach concentrated juice, 5-15 parts of pear concentrated juice and 1-9 parts of blueberry concentrated juice.

[0017] Preferably, additives are also included, specifically, by weight, 0.1-1.5 parts of konjac flour, 20-35 parts of oligofructose, and 5-15 parts of oligogalactose.

[0018] Preferably, it also includes flavoring agents, specifically, by weight, it also includes 0.1-1.5 parts of citric acid, 0.01-0.12 parts of stevioside and 0.1-0.5 parts of flavors.

[0019] A black fruit aronia fruit product for improving sleep and anti-oxidation, comprising, by weight, 25 parts of black fruit aronia juice, 1 part of gamma-aminobutyric acid, 1 part of tea theanine, 1 part of gastrodia elata, 0.5 parts of nutmeg, 0.5 parts of black plum, 0.1 parts of passion fruit extract, 0.1 parts of saffron extract, 20 parts of peach concentrated juice, 10 parts of pear concentrated juice, 5 parts of blueberry concentrated juice, 0.6 parts of konjac flour, 30 parts of oligofructose, 10 parts of galacto-oligosaccharide, 0.5 parts of citric acid, 0.06 parts of stevioside and 0.3 parts of flavor.

[0020] A method for preparing the product comprises the steps of uniformly mixing Aronia nigra juice, gamma-aminobutyric acid, tea theanine, Gastrodia elata, nutmeg, black plum, passion fruit extract and saffron extract with water and then sterilizing the mixture.

[0021] Preferably, the sterilization temperature is 80-100°C and the sterilization time is 30-50 minutes.

[0022] The above products can be used in foods or health products for calming nerves, promoting sleep and resisting oxidation.

[0023] Compared with existing sleep aid products, the present invention has the following advantages:

[0024] 1. In the sleep-improving and anti-oxidative Aronia nigra fruit product provided by the present invention, gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter in the central nervous system, and plays a key role in regulating the balance of excitation and inhibition of neurons in the central nervous system. GABA can affect the GABA content level and receptor expression in the central nervous system through the vagus nerve pathway in the gut-brain axis. GABA can also improve sleep through bidirectional communication pathways of the gut-brain axis such as the neuroendocrine pathway and the immune pathway. Intake of GABA can increase the activity of glucose phospholipase, thereby promoting energy metabolism in the animal brain, activating cerebral blood flow, increasing oxygen supply, and ultimately restoring brain cell function and improving nerve function.

[0025] Theanine in tea has the effects of relaxation, improving memory, lowering blood pressure, and protecting nerves. It can actively cooperate with GABA to relax the body and mind and enter a sleep state, thereby improving sleep quality and sleep time.

[0026] Gastrodia elata is sweet and mild in nature, and enters the liver meridian. The main active ingredients of Gastrodia elata are gastrodin, p-hydroxybenzyl alcohol, p-hydroxybenzaldehyde and other ingredients. Gastrodin can cause sleep waveforms in the alpha waves of the electroencephalogram of normal adults, and has the effects of calming the wind and relieving convulsions, calming and promoting sleep.

[0027] Nutmeg, also known as meat fruit and jade fruit, contains methyl allyl alcohol in volatile nutmeg oil, which can inhibit the central nervous system and prolong people's sleep time.

[0028] Black plums, which contain organic acids such as malic acid and citric acid, can promote the secretion of melatonin in the brain and help improve sleep quality. Black plum decoction can significantly prolong the sleep time of mice and has certain sedative, hypnotic and anticonvulsant effects.

[0029] Passionflower extract mainly contains alkaloids, volatile oils, flavonoids and other bioactive substances, which have various pharmacological effects such as anti-anxiety, sedation, sleep regulation, anticonvulsant and anti-epilepsy, etc. In other countries, in addition to using passionflower extract as a dietary supplement, passionflower extract is also used in combination with other herbs to treat anxiety, insomnia, sedation, etc.

[0030] The fruit of Aronia nigra, also known as wild aronia and immortal berry, contains not only carbohydrates, proteins, vitamins, carotenoids and trace elements, but also rich polyphenolic compounds such as proanthocyanidins, anthocyanidins, flavonoids, phenolic acids, etc. It has multiple functions such as anti-oxidation, anti-fatigue, anti-aging, and has certain medicinal value. Anthocyanidins, as a natural antioxidant, are composed of a large number of phenolic hydroxyl groups, which can block free radical chain reactions in reactions with active oxygen and play an antioxidant role. Due to the high content of polyphenolic compounds in Aronia nigra, especially anthocyanidins, Aronia nigra has a strong antioxidant capacity.

[0031] Saffron extract, also known as crocus, has shown that crocin has good free radical scavenging ability and reduces oxidative damage, thus helping to protect the body from oxidative stress.

[0032] The ingredients for improving sleep provided by the present invention are mainly γ-aminobutyric acid, tea theanine, gastrodia elata, nutmeg, ebony and passion fruit extracts, and the antioxidant ingredients are mainly aronia nigra juice and saffron extract. In the present invention, it is known from mouse animal experiments that aronia nigra juice and saffron extract can also affect the sleep effect of the product; it is known from DPPH free radical scavenging rate and hydroxyl free radical elimination rate experiments that gastrodia elata, nutmeg and ebony also have an effect on the free radical elimination rate, and the above components cooperate synergistically to achieve better sleep-aiding effect and antioxidant effect.

[0033] 2. The method for preparing Aronia nigra juice provided by the present invention adopts a combination of pectinase and cellulase to enzymolyze Aronia nigra fruits, thereby increasing the juice yield of Aronia nigra fruits, reducing the astringency of the Aronia nigra fruits themselves, improving the taste while retaining its effective ingredients to the maximum extent, effectively improving its antioxidant capacity, and making the obtained Aronia nigra juice clearer.

[0034] 3. The black fruit aronia fruit product for improving sleep and anti-oxidation provided by the present invention is added with peach concentrated juice, pear concentrated juice, blueberry concentrated juice, oligofructose, oligogalactose, citric acid and steviol glycoside and other flavoring substances, but the taste still has a very obvious Chinese medicine flavor. After adding black fruit aronia juice, the taste is softer, satisfying the consumer's demand for taste. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 This is a radar chart of sensory evaluation scores for aronia nut products. DETAILED DESCRIPTION

[0036] The following non-limiting examples can enable those skilled in the art to more fully understand the present invention, but do not limit the present invention in any way. The following content is merely an exemplary description of the scope of the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, which should also fall within the scope of the present invention.

[0037] The present invention is further described below by way of specific examples. The various chemical reagents used in the examples of the present invention are obtained through conventional commercial channels unless otherwise specified. Products from different manufacturers have no significant effect on the effects.

[0038] Among them, γ-aminobutyric acid was purchased from Daoqin Biotechnology (Shanghai) Co., Ltd. with a γ-aminobutyric acid of 98%; tea amino acids were purchased from Shanghai Nord Bio-Industry Co., Ltd.; Gastrodia elata was purchased from Xi'an Youshuo Biotechnology Co., Ltd. as Gastrodia elata extract, with a specification of 98% gastrodin; nutmeg was purchased from Shaanxi Huike Plant Development Co., Ltd. with a specification of 10:1; black plum was purchased from Shaanxi Huike Plant Development Co., Ltd. with a specification of 10:1; passion fruit extract was purchased from Xi'an Ruiying Biotechnology Co., Ltd. with a specification of passion fruit flavonoids 5%; saffron extract was purchased from Spanish plant extract ingredient supplier Natac; peach concentrate was purchased from Fujian Lvquan Food Co., Ltd. with a specification of TA: 1.5%±0.3%; pear concentrate was purchased from Fujian Lvquan Food Co., Ltd. with a specification of TA: ≥0.8%; blueberry concentrate was purchased from Daxinganling Lingbei Cold Zone Biotechnology Co., Ltd., with a Brix degree of 42; Cassia seed powder was purchased from Shaanxi Hengruikang Health Industry Co., Ltd. with a specification of 10:1; grape seed extract was purchased from Shaanxi Yiruinong Biotechnology Co., Ltd. with a specification of 95%.

[0039] Preparation Example 1 Aronia nigra juice

[0040] The present embodiment provides a black fruit aronia juice, and the preparation method thereof comprises the following steps: (1) selecting, washing, removing stems and squeezing black fruit aronia fruits to obtain black fruit aronia pulp; (2) adding an enzyme preparation to the black fruit aronia pulp for enzymolysis at 45°C for 3 hours, filtering out the pomace, and then inactivating the enzyme at 90°C for 5 minutes to obtain black fruit aronia juice. The amount of the enzyme preparation added is 0.05% of the weight of the black fruit aronia pulp, and the enzyme preparation is composed of cellulase (30000U / g) and pectinase (50000U / g) in a weight ratio of 5:4.

[0041] Example 1

[0042] This embodiment provides a black fruit aronia fruit product for improving sleep and anti-oxidation, including 25 parts of black fruit aronia juice, 1 part of γ-aminobutyric acid, 1 part of tea theanine, 1 part of gastrodia elata, 0.5 parts of nutmeg, 0.5 parts of black plum, 0.1 parts of passion fruit extract, 0.1 parts of saffron extract, 20 parts of peach concentrated juice, 10 parts of pear concentrated juice, 5 parts of blueberry concentrated juice, 0.6 parts of konjac flour, 30 parts of oligofructose, 10 parts of oligogalactose, 0.5 parts of citric acid, 0.06 parts of steviol glycoside, 0.3 parts of essence and 394 parts of water. The black fruit aronia juice is obtained in Preparation Example 1.

[0043] Its preparation method is:

[0044] (1) adding konjac flour, oligofructose, galacto-oligosaccharide, citric acid, steviol glycoside and essence into water and mixing and stirring uniformly to obtain a mixed phase A;

[0045] (2) adding aronia juice, peach concentrate juice, pear concentrate juice, and blueberry concentrate juice to the mixed phase A to obtain a mixed phase B;

[0046] (3) Add γ-aminobutyric acid, tea theanine, gastrodia elata, nutmeg, ebony, passion fruit extract and saffron extract to mixed phase B, mix and stir evenly to obtain mixed phase C, stir for 30 min, and sterilize at 90 °C for 30 min to obtain the black fruit glandular rib aronia fruit product.

[0047] Example 2

[0048] This embodiment provides a black fruit aronia fruit product for improving sleep and anti-oxidation, including 20 parts of black fruit aronia juice, 2 parts of γ-aminobutyric acid, 0.5 parts of tea theanine, 2 parts of gastrodia elata, 0.1 parts of nutmeg, 1 part of black plum, 0.05 parts of passion fruit extract, 0.15 parts of saffron extract, 12 parts of peach concentrated juice, 15 parts of pear concentrated juice, 1 part of blueberry concentrated juice, 1.5 parts of konjac flour, 20 parts of oligofructose, 15 parts of oligogalactose, 0.1 parts of citric acid, 0.12 parts of steviol glycoside, 0.1 parts of essence and 410 parts of water. The black fruit aronia juice is obtained in Preparation Example 1.

[0049] The preparation method is the same as that of Example 1.

[0050] Example 3

[0051] This embodiment provides a black fruit aronia fruit product for improving sleep and anti-oxidation, including 30 parts of black fruit aronia juice, 0.5 parts of γ-aminobutyric acid, 2 parts of tea theanine, 0.5 parts of gastrodia elata, 1 part of nutmeg, 0.1 parts of black plum, 0.15 parts of passion fruit extract, 0.05 parts of saffron extract, 25 parts of peach concentrated juice, 5 parts of pear concentrated juice, 9 parts of blueberry concentrated juice, 0.1 parts of konjac flour, 35 parts of oligofructose, 5 parts of oligogalactose, 1.5 parts of citric acid, 0.01 parts of steviol glycoside, 0.5 parts of essence and 385 parts of water. The black fruit aronia juice is obtained in Preparation Example 1.

[0052] The preparation method is the same as that of Example 1.

[0053] Example 4

[0054] This embodiment provides a black fruit aronia fruit product for improving sleep and anti-oxidation, including 22 parts of black fruit aronia juice, 1.2 parts of γ-aminobutyric acid, 0.8 parts of tea theanine, 1.2 parts of gastrodia elata, 0.3 parts of nutmeg, 0.6 parts of black plum, 0.08 parts of passion fruit extract, 0.13 parts of saffron extract, 16 parts of peach concentrated juice, 12 parts of pear concentrated juice, 4 parts of blueberry concentrated juice, 0.9 parts of konjac flour, 28 parts of oligofructose, 12 parts of oligogalactose, 0.2 parts of citric acid, 0.09 parts of steviol glycosides, 0.2 parts of essence and 400 parts of water. The black fruit aronia juice is obtained in Preparation Example 1.

[0055] The preparation method is the same as that of Example 1.

[0056] Example 5

[0057] This embodiment provides a black fruit aronia fruit product for improving sleep and anti-oxidation, including 28 parts of black fruit aronia juice, 0.7 parts of γ-aminobutyric acid, 1.2 parts of tea theanine, 0.8 parts of gastrodia elata, 0.6 parts of nutmeg, 0.3 parts of black plum, 0.12 parts of passion fruit extract, 0.1 parts of saffron extract, 22 parts of peach concentrate juice, 8 parts of pear concentrate juice, 7 parts of blueberry concentrate juice, 0.5 parts of konjac flour, 33 parts of oligofructose, 8 parts of oligogalactose, 0.8 parts of citric acid, 0.05 parts of steviol glycoside, 0.35 parts of essence and 388 parts of water. The black fruit aronia juice is obtained in Preparation Example 1.

[0058] The preparation method is the same as that of Example 1.

[0059] Comparative Example 1

[0060] This comparative example provides a sleep-improving and anti-oxidative Aronia nigra fruit product, which is different from Example 1 only in that Aronia nigra fruit juice is not included, but the total weight of γ-aminobutyric acid, tea theanine, Gastrodia elata, nutmeg, ebony plum, passion fruit extract and saffron extract is the same as the total weight of Aronia nigra fruit juice, γ-aminobutyric acid, tea theanine, Gastrodia elata, nutmeg, ebony plum, passion fruit extract and saffron extract in Example 1. The remaining components and preparation methods are the same as those in Example 1.

[0061] Comparative Example 2

[0062] This comparative example provides a sleep-improving and anti-oxidative Aronia nigra fruit product, which is different from Example 1 only in that Gastrodia elata is not included, but the total weight of Aronia nigra juice, γ-aminobutyric acid, tea theanine, nutmeg, ebony plum, passion fruit extract and saffron extract is the same as the total weight of Aronia nigra juice, γ-aminobutyric acid, tea theanine, Gastrodia elata, nutmeg, ebony plum, passion fruit extract and saffron extract in Example 1. The remaining components and preparation methods are the same as those in Example 1.

[0063] Comparative Example 3

[0064] This comparative example provides a sleep-improving and anti-oxidative Aronia nigra fruit product, which differs from Example 1 only in that nutmeg is not included, but the total weight of Aronia nigra juice, γ-aminobutyric acid, tea theanine, Gastrodia elata, ebony plum, passion fruit extract and saffron extract is the same as the total weight of Aronia nigra juice, γ-aminobutyric acid, tea theanine, Gastrodia elata, nutmeg, ebony plum, passion fruit extract and saffron extract in Example 1. The remaining components and preparation methods are the same as those in Example 1.

[0065] Comparative Example 4

[0066] This comparative example provides a sleep-improving and anti-oxidative Aronia nigra fruit product, which is different from Example 1 only in that black plum is not included, but the total weight of Aronia nigra juice, γ-aminobutyric acid, tea theanine, Gastrodia elata, nutmeg, passion fruit extract and saffron extract is the same as the total weight of Aronia nigra juice, γ-aminobutyric acid, tea theanine, Gastrodia elata, nutmeg, black plum, passion fruit extract and saffron extract in Example 1. The remaining components and preparation methods are the same as those in Example 1.

[0067] Comparative Example 5

[0068] This comparative example provides an Aronia nigra fruit product for improving sleep and anti-oxidation, which is different from Example 1 only in that nutmeg is replaced by Cassia seed powder, and the other components and preparation methods are the same as those in Example 1.

[0069] Comparative Example 6

[0070] This comparative example provides an Aronia nigra fruit product for improving sleep and anti-oxidation, which is different from Example 1 only in that the saffron extract is replaced by grape seed extract, and the other components and preparation methods are the same as those in Example 1.

[0071] Comparative Example 7

[0072] This comparative example provides a sleep-improving and anti-oxidative Aronia nigra fruit product, which is different from Example 1 only in that Aronia nigra juice, nutmeg and ebony are not within the preferred range, and includes 19 parts of Aronia nigra juice, 1 part of γ-aminobutyric acid, 1 part of tea theanine, 1 part of Gastrodia elata, 3 parts of nutmeg, 4 parts of ebony, 0.1 part of passion fruit extract, 0.1 part of saffron extract, 20 parts of peach concentrate juice, 10 parts of pear concentrate juice, 5 parts of blueberry concentrate juice, 0.6 parts of konjac flour, 30 parts of oligofructose, 10 parts of oligogalactose, 0.5 parts of citric acid, 0.06 parts of stevioside, 0.3 parts of essence and 394 parts of water. The preparation methods are the same as those in Example 1.

[0073] Comparative Example 8

[0074] This comparative example provides a black fruit Aronia oleracea fruit product for improving sleep and anti-oxidation, and the only difference between the comparative example and Example 1 is that the preparation method of the black fruit Aronia oleracea juice is different. In this comparative example, the preparation method of the black fruit Aronia oleracea juice is: selecting, washing, removing stems and squeezing the black fruit Aronia oleracea fruits to obtain black fruit Aronia oleracea pulp, and filtering out the pomace to obtain the black fruit Aronia oleracea juice.

[0075] Experimental Example 1

[0076] Experimental animals and groups:

[0077] KM male mice, weighing 18-22g, were randomly divided into 5 experimental groups, and the mouse weight observation experiment, direct sleep experiment, prolonged pentobarbital sleep experiment, pentobarbital sodium subthreshold dose hypnosis experiment and pentobarbital sodium sleep latency test were performed respectively, with 140 mice in each experimental group, and each experimental group was divided into blank control group, Example 1 group, Example 2 group, Example 3 group, Example 4 group, Example 5 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, Comparative Example 5 group, Comparative Example 6 group, Comparative Example 7 group and Comparative Example 8 group, a total of 14 groups, wherein the mice of Examples 1-5 and Comparative Examples 1-8 were gavaged with the corresponding products, and the dosage was 190 mg / kg·bw, and the blank control group was gavaged with normal saline, and each group of animals was gavaged once a day, and the gavage intervention was continued for 28 days. The weight of the mice was measured once a week.

[0078] Experimental methods

[0079] 1 Effects on mouse body weight

[0080] The body weights of mice were analyzed 7, 14, 21, and 28 days after oral administration of the Aronia nigra fruit product. The results are shown in Table 1.

[0081] 2. Direct sleep effect experiment in mice

[0082] Each group of mice was given the test sample every day, and the righting reflex disappeared for 60 s within 1 h after the last administration. The number and time of sleeping mice were recorded.

[0083] 3. Prolonged Pentobarbital Sleep Experiment

[0084] According to the Technical Specifications for Inspection and Evaluation of Health Food, 30 minutes after the last oral administration of the sample, each group of mice was intraperitoneally injected with 50 mg / kg·bw sodium pentobarbital, with an injection volume of 0.1 mL / 10 g·bw. The disappearance of the righting reflex of the mouse for more than 60 seconds was used as the criterion for falling asleep, and the time from the disappearance to the recovery of the righting reflex of the mouse was used as the sleeping time of the animal. The test substance was compared to see whether it prolonged the sleeping time of the mice injected with sodium pentobarbital.

[0085] 4. Subthreshold dose hypnosis experiment of sodium pentobarbital

[0086] According to the Technical Specifications for Inspection and Evaluation of Health Food, 30 minutes after the last oral administration of the sample, each group of mice was intraperitoneally injected with 30 mg / kg·bw sodium pentobarbital, with an injection volume of 0.1 mL / 10g·bw. The disappearance of the righting reflex of mice for more than 60 seconds was used as the criterion for falling asleep. The number of animals that fell asleep in each group within 30 minutes was recorded and the incidence of sleep was calculated. Incidence of sleep (%) = (number of animals that fell asleep / number of animals in each group) × 100. Compare whether the test substance increases the incidence of sleep in mice injected with subthreshold doses of sodium pentobarbital.

[0087] 5. Sodium barbital sleep latency test

[0088] According to the Technical Specifications for Inspection and Evaluation of Health Food, 20 minutes after the last oral administration of the sample, each group of mice was intraperitoneally injected with 280 mg / kg·bw sodium pentobarbital, with an injection volume of 0.1 mL / 10g·bw. The disappearance of the righting reflex of mice for more than 60 seconds was used as the criterion for falling asleep, and the time from the injection of sodium pentobarbital to the disappearance of the righting reflex was used as the sleep latency. The test substances were compared to see whether they could shorten the sleep latency of mice injected with sodium pentobarbital.

[0089] Experimental results: The results of body weight analysis are shown in Table 1.

[0090] Table 1 Effects of Aronia nigra fruit products on body weight of mice (g)

[0091]

[0092] The results of the prolonged sodium pentobarbital sleep test, the sodium pentobarbital subthreshold dose hypnosis test, and the sodium pentobarbital sleep latency test are shown in Table 2.

[0093] Table 2 Effects of Aronia nigra fruit products on sleep quality of insomnia mice

[0094]

[0095] Note: Compared with the blank control group, ** indicates P <0.01,* indicates P <0.05; compared with Example 1, ## indicates P <0.01, # indicates P <0.05.

[0096] Result analysis:

[0097] The weight of the experimental animals was analyzed before and after the intervention. The weight change reflects the growth status and health of the mice to a certain extent. As shown in Table 1, there was no significant difference in the initial weight of the mice in each group before the experiment ( P >0.05), and there was no significant difference in the body weight of mice in each group after oral administration for 7, 14, 21, and 28 days (P >0.05). During the experiment, the fur of mice in each group was shiny, and their diet and activities were normal, indicating that Examples 1-5 and Comparative Examples 1-8 had no significant effect on the weight of mice.

[0098] The results of the direct sleep experiment on mice show that after the sample was administered, the animals did not fall asleep, and the number of animals that fell asleep and the sleep time were both 0, indicating that the formula of the present invention has no direct sleep effect.

[0099] As shown in Table 2, compared with the blank control group, Examples 1-5 all have extremely significant differences ( P <0.01), there was no significant difference between Examples 1-5, which shows that Examples 1-5 can prolong the sleep time of mice, increase the incidence of sleep and shorten the sleep latency, which means that the product prepared by the present invention has a very good effect of calming the nerves and helping sleep.

[0100] All comparative examples were analyzed for significance with Example 1. The length of sleep time can evaluate the quality of sleep. From the data in Table 2, it can be seen that compared with Example 1, the sleep time of mice in Comparative Examples 1 and 6 was significantly shortened, indicating that Aronia nigra juice and saffron extract can affect the sleep time of mice in the entire product.

[0101] Compared with Example 1, the sleep time of mice in Comparative Examples 2-5 has extremely significant difference, indicating that Gastrodia elata, nutmeg and ebony plum in this product have a greater impact on the sleep of the entire product.

[0102] Compared with Example 1, Comparative Example 7 showed a significant difference in the sleep time of mice, indicating that the weight ratio of Aronia nigra juice, nutmeg and black plum can affect the sleep time of mice. Compared with Example 1, Comparative Example 8 showed no significant difference in the sleep of mice, indicating that the preparation method of Aronia nigra juice has little effect on the sleep effect of mice.

[0103] In terms of sleep incidence, Comparative Examples 1-8 are all lower than Example 1. In addition, sleep latency indicates the speed of falling asleep, and is also one of the indicators for evaluating sleep quality. Compared with Example 1, the sleep latency of Comparative Examples 1, 4, and 6 is significantly prolonged, Comparative Example 8 has no significant difference, and the sleep latency of the remaining comparative examples is extremely significantly different. This shows that the black fruit glandular rib aronia fruit product provided by the present invention can effectively improve the sleep quality of mice, and the components work together to achieve the effect of calming the nerves and helping sleep.

[0104] Experimental Example 2 Antioxidant Test

[0105] Test samples: products prepared in Examples 1-5 and Comparative Examples 1-8

[0106] (1) Determination of DPPH free radical scavenging rate

[0107] Take 2 mL of sample and add 2 mL of 0.2 mmol / L DPPH ethanol mixed solution, shake well, and let stand for 30 min in a dark place, then measure the absorbance value A1 at 517 nm. Replace DPPH solution with anhydrous ethanol solution as the blank group, and measure the absorbance value A2. Replace the sample solution with an equal volume of distilled water as the control group, and measure the absorbance value A0. The calculation formula for DPPH free radical scavenging rate is as follows:

[0108] DPPH free radical scavenging rate (%) = [1-(A1-A2) / A0] × 100%.

[0109] (2) Determination of hydroxyl radical scavenging rate.

[0110] Add 1 mL of 9 mmol / L ferrous sulfate solution, 1 mL of 9 mmol / L salicylic acid-ethanol solution, 1 mL of sample solution, and 1 mL of 8.8 mmol / L hydrogen peroxide solution to the colorimetric tube, and dilute to 10 mL with distilled water. Incubate at 37 °C for 30 min, use blank reagent as reference, and measure the absorbance value A1 at 510 nm. The absorbance value of the blank reagent is recorded as A0, and the hydroxyl radical scavenging rate is calculated as follows:

[0111] Hydroxyl free radical scavenging rate (%) = (A0-A1) / A0×100%

[0112] Experimental results:

[0113] Table 3 Antioxidant capacity of Aronia nigra fruit products

[0114]

[0115] Note: Compared with Example 1, ** indicates P <0.01,* indicates P <0.05

[0116] Result analysis:

[0117] As shown in Table 3, the DPPH free radical scavenging rate and hydroxyl free radical scavenging rate of Example 1 are the strongest, which are higher than those of other Examples 2-5 and Comparative Examples 1-8; there are no significant differences between the examples, and there are significant differences between Comparative Examples 1-8 and Example 1, among which the DPPH free radical scavenging rate and hydroxyl free radical scavenging rate of Comparative Example 1 are the weakest, and the antioxidant capacity is poor, indicating that the antioxidant capacity is significantly reduced after the lack of black fruit glandular rib aronia juice. When black fruit glandular rib aronia and other components work synergistically, the anthocyanin and anthocyanin active ingredients in the black fruit glandular rib aronia fruit can play a good antioxidant effect. Comparative Example 8 is significantly different from Example 1, indicating that the preparation method of black fruit glandular rib aronia juice affects the antioxidant capacity of the product, and the synergistic cooperation between the components of the product provided by the present invention achieves a better antioxidant capacity.

[0118] Experimental Example 3 Oral Sensation Evaluation

[0119] With reference to the sensory evaluation of compound beverages, a sensory evaluation table for Aronia nigra fruit products was developed, as shown in Table 4. Ten personnel trained in food sensory evaluation were selected to form a sensory evaluation team to evaluate the beverages prepared in Example 1, Comparative Example 1 and Comparative Example 8 from five aspects, respectively. Each sample was repeated 3 times, and the final results were averaged. The results are shown in Table 4. Figure 1 .

[0120] Table 4 Sensory scoring criteria

[0121]

[0122] The experimental results are as follows Figure 1 As shown, the sensory score of Example 1 is generally better than that of Comparative Example 1 and Comparative Example 8, indicating that after adding the black fruit aronia juice, the beverage has a uniform color and the aroma of black fruit aronia berries, and the black fruit aronia fruit has a rich taste and uniform tissue morphology after enzymatic hydrolysis. The black fruit aronia fruit product of the present invention is a healthy drink that integrates edible and health care functions, has certain sleep improvement and anti-oxidation effects, and can be liked and accepted by consumers.

[0123] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A sleep-improving and anti-oxidative Aronia nigra fruit product, characterized in that: Calculated by weight, the invention comprises 20-30 parts of aronia juice, 0.5-2 parts of gamma-aminobutyric acid, 0.5-2 parts of tea theanine, 0.5-2 parts of gastrodia elata, 0.1-1 parts of nutmeg, 0.1-1 parts of ebony, 0.05-0.15 parts of passionflower extract and 0.05-0.15 parts of saffron extract.

2. The product according to claim 1, characterized in that Calculated by weight, the invention comprises 22-28 parts of aronia juice, 0.7-1.2 parts of gamma-aminobutyric acid, 0.8-1.2 parts of tea theanine, 0.8-1.2 parts of gastrodia elata, 0.3-0.6 parts of nutmeg, 0.3-0.6 parts of ebony, 0.08-0.12 parts of passionflower extract and 0.1-0.13 parts of saffron extract.

3. The product according to claim 2, characterized in that The weight ratio of the Aronia nigra juice, nutmeg and black plum is (22-28): (0.3-0.6): (0.3-0.6).

4. The product according to claim 2, characterized in that The weight ratio of the Aronia nigra juice, gamma-aminobutyric acid, tea amino acids and Gastrodia elata is (22-28): (0.7-1.2): (0.8-1.2): (0.8-1.2).

5. The product according to any one of claims 1 to 4, characterized in that: The preparation method of the Aronia nigra juice comprises the following steps: adding an enzyme preparation to Aronia nigra pulp, performing enzymolysis at 40-50° C. for 3-4 hours, filtering out the pomace, and inactivating the enzyme to obtain Aronia nigra juice, wherein the enzyme preparation is added in an amount of 0.4-0.5%, and is composed of cellulase and pectinase in a weight ratio of 5:

4.

6. The product according to claim 1, characterized in that It also includes 12-25 portions of peach juice concentrate, 5-15 portions of pear juice concentrate and 1-9 portions of blueberry juice concentrate.

7. The product according to claim 1, characterized in that The invention also comprises 0.1-1.5 parts of konjac flour, 20-35 parts of oligofructose and 5-15 parts of oligogalactose; 0.1-1.5 parts of citric acid, 0.01-0.12 parts of stevioside and 0.1-0.5 parts of essence.

8. The product according to claim 7, comprising, by weight, 25 parts of aronia juice, 1 part of gamma-aminobutyric acid, 1 part of tea theanine, 1 part of gastrodia elata, 0.5 parts of nutmeg, 0.5 parts of ebony, 0.1 parts of passionflower extract and 0.1 parts of saffron extract, 20 parts of peach concentrate juice, 10 parts of pear concentrate juice, 5 parts of blueberry concentrate juice, 0.6 parts of konjac flour, 30 parts of oligofructose, 10 parts of galacto-oligosaccharide, 0.5 parts of citric acid, 0.06 parts of steviol glycoside and 0.3 parts of flavor.

9. A method for preparing the product according to any one of claims 1 to 8, characterized in that: The method comprises the steps of uniformly mixing aronia juice, gamma-aminobutyric acid, tea theanine, gastrodia elata, nutmeg, black plum, passion fruit extract and saffron extract with water and then sterilizing the mixture.

10. Use of the product according to any one of claims 1 to 8 in food or health products for calming nerves, promoting sleep and resisting oxidation.

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