Neural electrode modified by atomic-scale dispersed metal catalytic material at super normal temperature and preparation method

Abstract

The invention discloses a neural electrode modified by an atomic-scale dispersed metal catalytic material at an ultra-normal temperature and a preparation method thereof, and the preparation method comprises the following steps: carrying out roughening treatment, cleaning and drying on the surface of a metal wire to obtain a neural electrode substrate; coating and insulating other parts, except the electrode recording site, of the neural electrode substrate by using a polyimide solution; an atomic-scale dispersed metal catalytic material modified neural electrode interface is prepared in an ultra-normal-temperature environment in a constant-current mode of an electrochemical three-electrode system; according to the electrode, the effective contact area of an electrode interface can be increased, the electron transport capacity can be enhanced, the efficient biological catalytic activity of the atomic-scale dispersed metal catalytic material can be exerted, the inflammatory reaction of an electrode-tissue interface is reduced, scar tissue formation is reduced, the biocompatibility of the neural electrode is greatly improved, and the application prospect is wide. And the signal quality of long-term recording and the detection sensitivity are improved.

Description

Technical Field

[0001] The present invention belongs to the intersectional field of neural electrodes and materials, and relates to a neural electrode modified with atomic-level dispersed metal catalytic materials at super-normal temperature and a preparation method thereof. Background Art

[0002] Neural electrodes play a vital role in neuroscience research and the treatment of neurological diseases. They are the basic devices for realizing the interface between the brain and the machine. The key is to accurately monitor and manipulate the electrophysiological activities of the nervous system. With the advancement of science and technology, neural electrodes have played an important role in the diagnosis and treatment of neurological diseases, such as stroke, Parkinson's disease and epilepsy, because these diseases are usually closely related to abnormal neural electrical patterns in the brain. Although a series of breakthroughs and innovations have been made in this field, there are still some significant problems and limitations in the current clinical neural electrode systems, especially in terms of signal acquisition sensitivity and long-term stability.

[0003] Neural electrodes currently face huge challenges. Clinically, neural electrodes are required to ensure sufficiently low impedance and high signal acquisition sensitivity while not causing significant tissue damage during implantation. However, because the material stiffness of traditional implantable neural electrodes is much higher than the natural softness of brain tissue, a serious interface mismatch is formed between the electrode and brain tissue. This mismatch may not only trigger an inflammatory response, but may also cause damage to or even death of neurons, thereby reducing the therapeutic effect and signal quality. In addition, because the EEG signal itself is weak and the background noise is large, the signal frequency range is wide, and it is easily affected by environmental electromagnetic interference. At the same time, the low electron transfer efficiency and low charge injection capacity of traditional metal electrodes limit the electron transport between the tissue-electrode interface, further reducing the signal-to-noise ratio and acquisition sensitivity.

[0004] In view of the above challenges, future neural electrodes should focus on the development of new neural electrode materials and designs, aiming to improve the signal acquisition capabilities and long-term stability of the electrodes. Summary of the invention

[0005] The purpose of the present invention is to improve the shortcomings of existing neural electrodes and provide a neural electrode modified with atomic-level dispersed metal catalytic materials at super-normal temperatures.

[0006] The second object of the present invention is to provide a method for preparing a neural electrode modified with atomically dispersed metal catalytic materials at supernormal temperatures.

[0007] The technical solution of the present invention is summarized as follows:

[0008] A method for preparing a neural electrode modified with atomically dispersed metal catalytic materials at supernormal temperature comprises the following steps:

[0009] (1) roughening the surface of a metal wire with a diameter of 5 to 200 μm by sandpaper, ultrasonically cleaning it with deionized water, acetone, and anhydrous ethanol in sequence, and drying it to obtain a neural electrode substrate;

[0010] (2) preparing a polyimide solution, and using the polyimide solution to coat and insulate the other parts of the neural electrode substrate except the electrode recording site; or using an insulating sleeve to wrap and insulate the other parts of the neural electrode substrate except the electrode recording site;

[0011] (3) The neural electrode obtained in step (2) is used as a working electrode, a platinum electrode is used as a counter electrode, and Ag / AgCl is used as a reference electrode, and connected to an electrochemical workstation to form a three-electrode system. A dispersion of an atomically dispersed metal catalytic material with a concentration of 0.2-5 mg / mL is added to the electrolytic cell as an electrodeposition working solution, so that the recording site is submerged, and constant current deposition is performed under an ultra-normal temperature environment to uniformly deposit the atomically dispersed metal catalytic material on the surface of the recording site, and then dried to obtain a neural electrode modified with an atomically dispersed metal catalytic material at an ultra-normal temperature;

[0012] The preparation steps of the atomically dispersed metal catalytic material dispersion liquid with a concentration of 0.2-5 mg / mL are as follows: 0.2-5 mg of the atomically dispersed metal catalytic material is added into 1 mL of deionized water, and the atomically dispersed metal catalytic material is uniformly dispersed while being ultrasonically stirred to obtain the atomically dispersed metal catalytic material dispersion liquid with a concentration of 0.2-5 mg / mL;

[0013] The super-normal temperature environment is a constant temperature maintained at 50-90°C.

[0014] The metal wire is preferably gold, silver, copper, platinum, tungsten, iridium, nickel, platinum-iridium alloy, nickel-titanium alloy, tungsten-platinum alloy, nickel-cobalt alloy or stainless steel.

[0015] The mass concentration of the polyimide solution is preferably 30%-50%.

[0016] Preferably, the weight average molecular weight of the polyimide is 10,000-100,000; and the solvent of the polyimide solution is N,N-dimethylformamide, dimethyl sulfoxide, tetrahydrofuran or acetone.

[0017] Preferably, the atomically dispersed metal catalytic material is a bimetallic single-atom nanozyme M1M2 MANs or a metal-organic framework material M MOFs.

[0018] In the above-mentioned bimetallic single-atom nanozyme M1M2 MANs, M1 is selected from Fe, Cu, Rh, V, Pd, Pt, Ni, Mn, Zn, Ru, Ir, Cr, Zr, Mo, Re, Au, Cd, Tb, W, Ce or Co;

[0019] The M2 is selected from Fe, Cu, Rh, V, Pd, Pt, Ni, Mn, Zn, Ru, Ir, Cr, Zr, Mo, Re, Au, Cd, Tb, W, Ce or Co, and the M1 is different from M2.

[0020] The M in MMOFs is selected from Fe, Cu, Rh, V, Pd, Pt, Ni, Mn, Zn, Ru, Ir, Cr, Zr, Mo, Re, Au, Cd, Tb, W, Ce or Co.

[0021] The frequency of the ultrasound in step (3) is preferably 40 kHz, and the ultrasound time is 5-30 min.

[0022] Preferably, the deposition current of the constant current deposition is 0.01-0.5 A, and the deposition time is 100-1800 s.

[0023] The preparation method mentioned above prepares a neural electrode modified with atomically dispersed metal catalytic material at ultra-normal temperature.

[0024] Advantages of the present invention:

[0025] Atomically dispersed metal catalytic materials have stable and efficient biocatalytic activity, and the structure and catalytic active center of atomically dispersed metal catalytic materials can be precisely regulated. They have high selectivity for catalytic reactions and can maintain stable structure and activity for a long time. The raw materials for the synthesis of this material are easy to obtain, and the synthesis method is simple. At the same time, the present invention modifies the neural electrode in an ultra-normal temperature environment. In an ultra-normal temperature environment, the molecular thermal motion is more intense and the electrochemical efficiency is higher. Therefore, using atomically dispersed metal catalytic materials to modify the neural electrode interface at ultra-normal temperatures can not only increase the effective contact area of ​​the electrode interface and enhance the electron transport capacity, but also make the material more uniformly modified at the neural electrode recording site, thereby extending the service life of the electrode. Atomically dispersed metal catalytic materials can exert their biocatalytic activity in a long-term and stable manner, which helps to improve the long-term stability of the neural electrode interface, and improve the signal quality and detection sensitivity of long-term recordings. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 Schematic diagram of the electrodeposition device.

[0027] Figure 2 The electrochemical properties of a neural electrode modified with atomically dispersed metal catalytic materials at ultra-normal temperatures.

[0028] Figure 3 Structural characterization of a neural electrode modified with atomically dispersed metal catalytic materials at ultra-high temperatures.

[0029] Figure 4The electrophysiological signal acquisition performance of a neural electrode modified with atomically dispersed metal catalytic material at ultra-normal temperature. DETAILED DESCRIPTION

[0030] Preparation of bimetallic single-atom nanozymes M1M2 MANs Reference: Xiaoyu Liu, Zhen Wan, Ke Chen, et al. Mated-Atom Nanozymes with Efficient Assisted NAD + Replenishment for SkinRegeneratio n.Nano Lett.2024 24(16),4924-4935.

[0031] Preparation of metal organic framework materials M MOFs reference: W. Wang, L. Zhang, Z. Liu, et al. Selective Methionine Pool Exhaustion Mediated by a Sequential Positioned MOF Nanotransformer for Intense Cancer Immunotherapy. Adv. Mater. 2023, 35, 2211866.

[0032] Electrodeposition equipment Figure 1 In the figure, 1 is a constant temperature device; 2 is an atomic-level dispersed metal catalytic material dispersion; 3 is an electrolytic cell; 4 is a reference electrode; 5 is a working electrode; 6 is a counter electrode; and 7 is an electrochemical workstation.

[0033] The present invention will be further described below by means of specific embodiments.

[0034] The neural electrode modified with atomically dispersed metal catalytic materials at supernormal temperatures of the present invention includes but is not limited to a single needle or array electrode.

[0035] Example 1

[0036] A method for preparing a neural electrode modified with atomically dispersed metal catalytic materials at supernormal temperature comprises the following steps:

[0037] (1) The surface of a platinum-iridium alloy wire with a diameter of 100 μm was roughened by grinding with 500-mesh sandpaper, and ultrasonically cleaned with deionized water, acetone, and anhydrous ethanol in sequence, and dried to obtain a neural electrode substrate;

[0038] (2) preparing a polyimide solution with a mass concentration of 40% (the weight average molecular weight of the polyimide is 10,000-50,000, and the solvent is N,N-dimethylformamide), and using the polyimide solution to coat and insulate the other parts of the neural electrode substrate except the electrode recording site; (or using an insulating sleeve to wrap and insulate the other parts of the neural electrode substrate except the electrode recording site);

[0039] (3) The neural electrode obtained in step (2) is used as a working electrode, a platinum electrode is used as a counter electrode, and Ag / AgCl is used as a reference electrode, and connected to an electrochemical workstation to form a three-electrode system. A 1 mg / mL atomically dispersed metal catalytic material (FeCu MAN) dispersion is added to the electrolytic cell as an electrodeposition working solution, so that the recording site is submerged, and constant current deposition (deposition current is 0.1 A, deposition time is 900 s) is performed under an ultra-normal temperature (constant temperature is maintained at 70° C.) environment to uniformly deposit the atomically dispersed metal catalytic material on the surface of the recording site, and then dried to obtain a neural electrode modified with an atomically dispersed metal catalytic material at an ultra-normal temperature;

[0040] The preparation steps of the atomically dispersed metal catalytic material (FeCu MAN) dispersion with a concentration of 1 mg / mL are as follows: 1 mg of FeCu MAN is added to 1 mL of deionized water, and stirred while ultrasonicating (ultrasonic frequency 40 kHz, ultrasonic time 10 min) to uniformly disperse the atomically dispersed metal catalytic material to obtain an atomically dispersed metal catalytic material dispersion with a concentration of 1 mg / mL.

[0041] Example 2

[0042] A method for preparing a neural electrode modified with atomically dispersed metal catalytic materials at supernormal temperature comprises the following steps:

[0043] (1) and (2) are the same as those in Example 1 (1) and (2);

[0044] (3) The neural electrode obtained in step (2) is used as a working electrode, a platinum electrode is used as a counter electrode, and Ag / AgCl is used as a reference electrode. The electrodes are connected to an electrochemical workstation to form a three-electrode system. A 1 mg / mL atomically dispersed metal catalytic material (Cu MOF) dispersion is added to the electrolytic cell as an electrodeposition working solution, so that the recording site is submerged. Constant current deposition (deposition current of 0.1 A, deposition time of 900 s) is performed under an ultra-normal temperature (constant temperature maintained at 70° C.) environment to uniformly deposit the atomically dispersed metal catalytic material on the surface of the recording site, and the electrode is dried to obtain a neural electrode modified with an atomically dispersed metal catalytic material at an ultra-normal temperature;

[0045] The preparation steps of an atomically dispersed metal catalytic material (Cu MOF) dispersion with a concentration of 1 mg / mL are as follows: 1 mg CuMOF is added to 1 mL deionized water, and stirred while ultrasonicating (ultrasonic frequency 40 kHz, ultrasonic time 10 min) to uniformly disperse the atomically dispersed metal catalytic material to obtain an atomically dispersed metal catalytic material dispersion with a concentration of 1 mg / mL.

[0046] Experiments have shown that by replacing the platinum-iridium alloy in this embodiment with gold, silver, platinum, tungsten, iridium, nickel, nickel-titanium alloy, tungsten-platinum alloy, nickel-cobalt alloy or stainless steel, and the other parts being the same as in this embodiment, a neural electrode modified with atomically dispersed metal catalytic materials can be prepared respectively;

[0047] The Cu in the Cu MOF of this embodiment can be replaced by Fe, Rh, Pd, Pt, Ni, Zn, Ru, Ir, Cr, Zr, Mo, Re, Au, Cd, Tb, W, Ce or Co, such as Fe MOF, Rh MOF, Pt MOF, Ni MOF, Zn MOF, Ru MOF, Ir MOF, Cr MOF, ZrMOF, Mo MOF, Re MOF, Au MOF, Cd MOF, Tb MOF, W MOF, Ce MOF, Co MOF, etc., and the rest are the same as in this embodiment, to prepare a corresponding neural electrode modified with an atomically dispersed metal catalytic material.

[0048] Example 3

[0049] A method for preparing a neural electrode modified with atomically dispersed metal catalytic materials at supernormal temperature comprises the following steps:

[0050] (1) The surface of a gold wire with a diameter of 200 μm was roughened by grinding with 500-mesh sandpaper, and ultrasonically cleaned with deionized water, acetone, and anhydrous ethanol in sequence, and dried to obtain a neural electrode substrate;

[0051] (2) preparing a polyimide solution (weight average molecular weight of polyimide is 50,000-80,000, solvent is dimethyl sulfoxide) (mass concentration is 30%), and using the polyimide solution to coat and insulate the other parts of the neural electrode substrate except the electrode recording site; (or using an insulating sleeve to wrap and insulate the other parts of the neural electrode substrate except the electrode recording site);

[0052] (3) The neural electrode obtained in step (2) is used as a working electrode, a platinum electrode is used as a counter electrode, and Ag / AgCl is used as a reference electrode, and connected to an electrochemical workstation to form a three-electrode system. A 0.2 mg / mL atomically dispersed metal catalytic material (FeCo MAN) dispersion is added to the electrolytic cell as an electrodeposition working solution, so that the recording site is submerged. Constant current deposition (deposition current of 0.5 A, deposition time of 1800 s) is performed under an ultra-normal temperature (constant temperature maintained at 50° C.) environment to uniformly deposit the atomically dispersed metal catalytic material on the surface of the recording site, and then dried to obtain a neural electrode modified with an atomically dispersed metal catalytic material at an ultra-normal temperature;

[0053] The preparation steps of the atomically dispersed metal catalytic material (FeCo MAN) dispersion with a concentration of 0.2 mg / mL are as follows: 0.2 mg FeCo MAN is added to 1 mL deionized water, and stirred while ultrasonicating (ultrasonic frequency 40 kHz, ultrasonic time 5 min) to uniformly disperse the atomically dispersed metal catalytic material to obtain an atomically dispersed metal catalytic material dispersion with a concentration of 0.2 mg / mL.

[0054] Example 4

[0055] A method for preparing a neural electrode modified with atomically dispersed metal catalytic materials at supernormal temperature comprises the following steps:

[0056] (1) The surface of a copper wire with a diameter of 5 μm was roughened by grinding with 500-mesh sandpaper, and ultrasonically cleaned with deionized water, acetone, and anhydrous ethanol in sequence, and dried to obtain a neural electrode substrate;

[0057] (2) preparing a polyimide solution (weight average molecular weight of polyimide is 80,000-100,000, solvent is tetrahydrofuran, acetone can also be used) (mass concentration is 50%), and using the polyimide solution to coat and insulate the other parts of the neural electrode substrate except the electrode recording site; (or using an insulating sleeve to wrap and insulate the other parts of the neural electrode substrate except the electrode recording site);

[0058] (3) The neural electrode obtained in step (2) is used as a working electrode, a platinum electrode is used as a counter electrode, and Ag / AgCl is used as a reference electrode. The electrodes are connected to an electrochemical workstation to form a three-electrode system. A 5 mg / mL atomically dispersed metal catalytic material (FeMn MAN) dispersion is added to the electrolytic cell as an electrodeposition working solution, so that the recording site is submerged. Constant current deposition (deposition current of 0.01 A, deposition time of 100 s) is performed under an ultra-normal temperature (constant temperature maintained at 90° C.) environment to uniformly deposit the atomically dispersed metal catalytic material on the surface of the recording site, and the electrode is dried to obtain a neural electrode modified with an atomically dispersed metal catalytic material at an ultra-normal temperature;

[0059] The preparation steps of the atomically dispersed metal catalytic material (FeMn MAN) dispersion with a concentration of 5 mg / mL are as follows: 5 mg of FeMn MAN is added to 1 mL of deionized water, and stirred while ultrasonicating (ultrasonic frequency of 40 kHz, ultrasonic time of 30 min) to uniformly disperse the atomically dispersed metal catalytic material to obtain an atomically dispersed metal catalytic material dispersion with a concentration of 5 mg / mL;

[0060] Experiments have shown that by replacing the copper in this embodiment with silver, platinum, tungsten, iridium, nickel, nickel-titanium alloy, tungsten-platinum alloy, nickel-cobalt alloy or stainless steel, and the other aspects being the same as in this embodiment, a neural electrode modified with atomically dispersed metal catalytic materials can be prepared;

[0061] Cu, Rh, V, Pd, Pt, Ni, Mn, Zn, Ru, Ir, Cr, Zr, Mo, Re, Au, Cd, Tb, W, Ce or Co (M1 in M1M2MANs) are used to replace Fe in this embodiment respectively;

[0062] The Mn in this embodiment is replaced by Fe, Rh, V, Pd, Pt, Ni, Zn, Ru, Ir, Cr, Zr, Mo, Re, Au, Cd, Tb, W or Ce (M2 in M1M2 MANs);

[0063] M1M2 MANs are VFe MAN, PdRh MAN, PtNi MAN, NiZn MAN, ZnCr MAN, IrZr MAN, RePtMAN, AuIr MAN, CdMo MAN, CrCu MAN, ZrCu MAN, TbCe MAN, WTb MAN, CeW MAN, PdRe MAN, PtAu MAN, NiCd MAN, RhV MAN, MnRu MAN, RhMn MAN, CoRu MAN, RhCo MAN, CuRh MAN, MoRuMAN, RhRu MAN, RuPd MAN, etc. The rest are the same as in the present embodiment; a corresponding neural electrode modified by atomically dispersed metal catalytic materials at super-normal temperature is prepared.

[0064] Comparative Example 1

[0065] A method for preparing a neural electrode interface modified with atomically dispersed metal catalytic materials at room temperature comprises the following steps:

[0066] (1) and (2) are the same as steps (1) and (2) of Example 1;

[0067] (3) The neural electrode obtained in step (2) is used as a working electrode, a platinum electrode is used as a counter electrode, and Ag / AgCl is used as a reference electrode. The electrodes are connected to an electrochemical workstation to form a three-electrode system. A 1 mg / mL atomically dispersed metal catalytic material (FeCu MAN) dispersion is added to the electrolytic cell as an electrodeposition working solution so as to cover the recording site. Constant current deposition (deposition current of 0.1 A, deposition time of 900 s) is performed at room temperature (maintained at 27° C.) to uniformly deposit the atomically dispersed metal catalytic material on the surface of the recording site. The electrode is dried to obtain a neural electrode modified with an atomically dispersed metal catalytic material at room temperature. The corresponding preparation steps of the atomically dispersed metal catalytic material (FeCu MAN) dispersion are the same as those in Example 1.

[0068] Comparative Example 2

[0069] A method for preparing a neural electrode interface modified with atomically dispersed metal catalytic materials at room temperature comprises the following steps:

[0070] (1) and (2) are the same as steps (1) and (2) of Example 2;

[0071] (3) The neural electrode obtained in step (2) is used as a working electrode, a platinum electrode is used as a counter electrode, and Ag / AgCl is used as a reference electrode. The electrodes are connected to an electrochemical workstation to form a three-electrode system. A 1 mg / mL atomically dispersed metal catalytic material (Cu MOF) dispersion is added to the electrolytic cell as an electrodeposition working solution so that the recording site is submerged. Constant current deposition (deposition current of 0.1 A, deposition time of 900 s) is performed at room temperature (maintained at 27° C.) to uniformly deposit the atomically dispersed metal catalytic material on the surface of the recording site. The electrode is dried to obtain a neural electrode modified with an atomically dispersed metal catalytic material at room temperature. The corresponding preparation steps of the atomically dispersed metal catalytic material (Cu MOF) dispersion are the same as those in Example 2.

[0072] Comparative Example 3

[0073] The neural electrode prepared in steps (1) and (2) of Example 1.

[0074] Experimental Example 1

[0075] The electrochemical performance tests were performed on the neural electrodes prepared in Example 1, Example 2, Comparative Example 1, Comparative Example 2 and Comparative Example 3.

[0076] The electrochemical impedance results of the above five neural electrodes show that the impedance of the neural electrodes prepared in Examples 1 and 2 is significantly reduced, and the impedance is reduced by more than 50% at the characteristic frequency of 1000 Hz, while the impedance of the neural electrode prepared in Comparative Example 1 is only reduced by 24%. The reduction in impedance is conducive to improving the acquisition sensitivity of the neural electrodes. Figure 2In the cyclic voltammetry test, the area enclosed by the curve of the neural electrode prepared in Example 1, Example 2 and Comparative Example 1 is significantly increased, and Example 1 and Example 2 are more significant, and the charge storage capacity is significantly improved, as shown in FIG. Figure 2 In summary, the neural electrodes modified by ultra-normal temperature atomically dispersed metal catalytic materials can significantly outperform the neural electrodes modified by atomically dispersed metal catalytic materials under normal temperature.

[0077] Experimental Example 2

[0078] The surface structures of the nerve electrodes prepared in Examples 1, 2 and Comparative Example 3 were characterized. The surface structures of the nerve electrodes were characterized using a scanning electron microscope. The results showed that the surface of the nerve electrode prepared in Comparative Example 3 was smooth without significant protrusions, the surface of the nerve electrode prepared in Example 1 had a sheet-like structure, and the surface of the nerve electrode prepared in Example 2 had uniform spherical nanoparticles. Figure 3 This structure can increase the contact area between the neural electrode and the brain tissue, increase the active sites, provide more channels for the transmission of electrical signals, and help improve the biocompatibility of the neural electrode and the signal acquisition quality.

[0079] Experimental Example 3:

[0080] A biological application of a neural electrode modified with an atomically dispersed metal catalytic material comprises the following steps:

[0081] (1) Adult Wistar male mice were selected and the skin was prepared after anesthesia. The skin from behind the eyes to in front of the ears was exposed. The mice were fixed on a stereotaxic apparatus and the skull was exposed. The anterior and posterior fontanelles and the location of the neural electrode implantation (CA1: +3.5 mm anterior and posterior; ±2.5 mm lateral; 2 mm depth) were determined and a skull drill was used to drill a hole in the skull.

[0082] (2) After the neural electrode prepared in Example 1 (implanted into the left CA1) and the neural electrode prepared in Comparative Example 3 (implanted into the right CA1) were stabilized for 5 minutes, the electrophysiological signals of specific brain regions were recorded using an Intan electrophysiological signal recording device, and the signals were analyzed and processed to compare the signal recording capabilities of the two electrodes.

[0083] Experiments have shown that the amplitude of the local field potential signal collected by the neural electrode prepared in Example 1 is larger, and the energy of the signal is stronger in frequency domain analysis. At the same time, the neural electrode prepared in Example 1 can collect neural signals that the neural electrode prepared in Comparative Example 3 cannot collect.

[0084] Results (see Figure 4) was analyzed and it was found that the amplitude of the local field potential signal collected by the neural electrode prepared in Example 1 was higher than that of the neural electrode prepared in Comparative Example 3. At the same time, through time-frequency domain analysis, it was found that the signal energy collected by the neural electrode prepared in Comparative Example 3 was much lower than that of the neural electrode prepared in Example 1. This shows that the neural electrode prepared in Example 1 is superior to the neural electrode prepared in Comparative Example 3 in the performance of collecting local field potential signals, which helps to provide more possibilities in the field of neural engineering.

[0085] In this experimental example, the animal models include but are not limited to healthy adult mice, rabbits, epilepsy model mice, Parkinson's model mice, etc.

[0086] Specific brain regions for implantation include but are not limited to CA1, CA3, M1, M2, etc.

[0087] Experiments have shown that the neural electrodes prepared in Examples 2, 3, and 4 respectively replace the neural electrodes prepared in Example 1 of this experimental example, and other aspects are the same as this experimental example. Analysis shows that the signal collection capabilities of the neural electrodes prepared in Examples 2, 3, and 4 are similar to that of the neural electrodes prepared in Example 1.

Claims

1. A method for preparing a neural electrode modified with atomically dispersed metal catalytic materials at supernormal temperatures, characterized in that The steps include: (1) roughening the surface of a metal wire with a diameter of 5 to 200 μm by sandpaper, ultrasonically cleaning it with deionized water, acetone, and anhydrous ethanol in sequence, and drying it to obtain a neural electrode substrate; (2) preparing a polyimide solution, and using the polyimide solution to coat and insulate the other parts of the neural electrode substrate except the electrode recording site; or using an insulating sleeve to wrap and insulate the other parts of the neural electrode substrate except the electrode recording site; (3) The neural electrode obtained in step (2) is used as a working electrode, a platinum electrode is used as a counter electrode, and Ag / AgCl is used as a reference electrode, and connected to an electrochemical workstation to form a three-electrode system. A dispersion of an atomically dispersed metal catalytic material with a concentration of 0.2-5 mg / mL is added to the electrolytic cell as an electrodeposition working solution, so that the recording site is submerged, and constant current deposition is performed under an ultra-normal temperature environment to uniformly deposit the atomically dispersed metal catalytic material on the surface of the recording site, and then dried to obtain a neural electrode modified with an atomically dispersed metal catalytic material at an ultra-normal temperature; The preparation steps of the atomically dispersed metal catalytic material dispersion liquid with a concentration of 0.2-5 mg / mL are as follows: 0.2-5 mg of the atomically dispersed metal catalytic material is added into 1 mL of deionized water, and the atomically dispersed metal catalytic material is uniformly dispersed while being ultrasonically stirred to obtain the atomically dispersed metal catalytic material dispersion liquid with a concentration of 0.2-5 mg / mL; The super-normal temperature environment is a constant temperature maintained at 50-90°C.

2. The preparation method according to claim 1, characterized in that The metal wire is gold, silver, copper, platinum, tungsten, iridium, nickel, platinum-iridium alloy, nickel-titanium alloy, tungsten-platinum alloy, nickel-cobalt alloy or stainless steel.

3. The preparation method according to claim 1, characterized in that The mass concentration of the polyimide solution is 30%-50%.

4. The preparation method according to claim 1 or 3, characterized in that the weight average molecular weight of the polyimide is 10,000-100,000; and the solvent of the polyimide solution is N,N-dimethylformamide, dimethyl sulfoxide, tetrahydrofuran or acetone.

5. The preparation method according to claim 1, characterized in that The atomic-level dispersed metal catalytic material is a bimetallic single-atom nanozyme M1M2 MANs or a metal-organic framework material M MOFs.

6. The preparation method according to claim 5, characterized in that In the bimetallic single-atom nanozyme M1M2 MANs, M1 is selected from Fe, Cu, Rh, V, Pd, Pt, Ni, Mn, Zn, Ru, Ir, Cr, Zr, Mo, Re, Au, Cd, Tb, W, Ce or Co; M2 is selected from Fe, Cu, Rh, V, Pd, Pt, Ni, Mn, Zn, Ru, Ir, Cr, Zr, Mo, Re, Au, Cd, Tb, W, Ce or Co, and M1 and M2 are different.

7. The preparation method according to claim 5, characterized in that The M in the MMOFs is selected from Fe, Cu, Rh, V, Pd, Pt, Ni, Mn, Zn, Ru, Ir, Cr, Zr, Mo, Re, Au, Cd, Tb, W, Ce or Co.

8. The preparation method according to claim 1, characterized in that The ultrasound frequency in step (3) is 40 kHz, and the ultrasound time is 5-30 min.

9. The preparation method according to claim 1, characterized in that The deposition current of the constant current deposition is 0.01-0.5A, and the deposition time is 100-1800s.

10. A neural electrode modified with atomically dispersed metal catalytic materials at supernormal temperatures, prepared by the preparation method of any one of claims 1 to 9.

Images