Antioxidant fiber mask containing tremella aurantialba polysaccharide

Abstract

The invention discloses an anti-oxidation fiber mask containing tremella aurantialba polysaccharide. The anti-oxidation fiber mask containing the tremella aurantialba polysaccharide is prepared from the following components in parts by weight: 6 to 30 parts of the tremella aurantialba polysaccharide, 10 to 45 parts of micromolecular collagen peptide and 30 to 80 parts of deionized water. The tremella aurantialba polysaccharide mask is prepared from the tremella aurantialba polysaccharide and the small-molecule collagen peptide according to a specific proportion, the tremella aurantialba polysaccharide serves as an exogenous antioxidant nutrient of an organism and can play a role in scavenging free radicals, and in addition, the tremella aurantialba polysaccharide has good biocompatibility and can be better attached to a mask, so that the mask is free of toxic and side effects. The anti-oxidation mask provides required nutrition for skin while resisting oxidation, and plays a positive role in regulating skin micro-ecology.

Description

Technical Field

[0001] The invention relates to the technical field of facial mask preparation, and in particular to an antioxidant fiber facial mask containing Tremella fuciformis polysaccharide. Background Art

[0002] Facial mask is a kind of beauty and skin care product applied on the face. It uses the mask as a carrier to absorb liquids with various skin care effects. As the mask liquid penetrates into the skin, the skin care effect is achieved. As people's demand for anti-oxidation increases, there are many types of anti-oxidation masks. However, in order to maintain the efficacy and preservation of the mask liquid, most antioxidant masks will add preservatives during preparation, which will result in too high a total amount of preservatives in the mask liquid. In addition, the mask base fabric on the market is mainly non-woven material. The thickness and porosity of the non-woven material directly affect the liquid holding capacity of the base fabric, resulting in the mask liquid unable to fully penetrate into the mask, low utilization rate, and unable to be fully absorbed by the skin, which in turn leads to itching, acne, and even local allergic redness and swelling symptoms, or the efficacy is relatively single and cannot meet people's daily maintenance needs.

[0003] Studies have shown that golden ear is rich in active polysaccharides, vitamins, minerals, proteins and amino acids. Golden ear polysaccharides have a high ability to scavenge hydroxyl free radicals. As an exogenous antioxidant nutrient, it prevents free radicals from damaging the body and cell tissues. In addition, golden ear polysaccharides have good biological activity and biocompatibility. They can interact with other polysaccharide components and fibrin in the skin in the extracellular matrix to generate an extracellular colloidal matrix containing a large amount of water, which plays a positive regulatory role in the skin microecology. It also has great market potential in the field of skin care. Based on this, how to apply golden ear polysaccharides to the preparation of facial masks and develop a facial mask that is antioxidant, easily absorbable and can meet daily skin care needs is one of the current research directions. Summary of the invention

[0004] The present invention aims to solve at least one of the technical problems existing in the prior art, and provides an antioxidant fiber mask containing Tremella fuciformis polysaccharide.

[0005] The present invention solves the technical problem by adopting the following technical solution: The present invention firstly provides an antioxidant fiber facial mask containing Tremella fuciformis polysaccharide. The antioxidant fiber facial mask containing Tremella fuciformis polysaccharide comprises, by weight: 6 to 30 parts of Tremella fuciformis polysaccharide, 10 to 45 parts of small molecule collagen peptides, and 30 to 80 parts of deionized water.

[0006] Preferably, the antioxidant fiber mask containing Tremella fuciformis polysaccharide comprises, by weight: 8 to 15 parts of Tremella fuciformis polysaccharide, 12 to 30 parts of small molecule collagen peptides, and 60 to 80 parts of deionized water.

[0007] Most preferably, the antioxidant fiber mask containing Tremella fuciformis polysaccharide comprises, by weight: 10 parts of Tremella fuciformis polysaccharide, 15 parts of small molecule collagen peptides, and 75 parts of deionized water.

[0008] Preferably, the method for extracting Tremella fuciformis polysaccharide comprises the following steps: S11. Pretreatment of Tremella fruiting bodies The Tremella fuciformis fruiting bodies were washed, dried to constant weight, crushed through a 40-80 mesh sieve, added with 12-18 times the weight of 92-98% ethanol solution, soaked for 12-36 hours, and filtered to obtain filtrate A and precipitate A; 12-18 times the volume of 92-98% ethanol solution was added to precipitate A, soaked for 12-36 hours, and filtered again to obtain filtrate B and precipitate B, and precipitate B was naturally air-dried to constant weight to obtain an experimental sample.

[0009] S12, Enzyme-assisted Extraction of Tremella Polysaccharide The experimental sample is dissolved in 70-90 times the weight of deionized water, the temperature is controlled at 75-85°C, and the extraction is carried out for 0.5-1.5h. After cooling to 45-55°C, 7.02-7.12% of the complex enzyme is added, wherein the complex enzyme is composed of cellulase, papain and pectinase in a weight ratio of 1:1: (0.65-0.85).

[0010] After 30-60 minutes of enzymatic hydrolysis, the centrifugal speed is set to 7000-9000 r / min, the enzymatic hydrolyzate is centrifuged for 20-40 minutes, the supernatant is taken, anhydrous ethanol is added to the supernatant until the concentration of the ethanol solution is 60-80%, and alcohol precipitation is carried out at 4°C for 18-30 hours, and then centrifuged at a speed of 7000-9000 r / min for 8-12 minutes, the precipitate is taken, and freeze-dried to obtain Tremella fuciformis polysaccharide.

[0011] Preferably, the molecular weight of the Tremella fuciformis polysaccharide is 200-2000 kD.

[0012] Most preferably, the molecular weight of the Tremella fuciformis polysaccharide is 500-800 kD.

[0013] Preferably, the preparation method of the antioxidant fiber mask containing Tremella fuciformis polysaccharide is: Tremella fuciformis polysaccharide, small molecule collagen peptide and water are mixed, stirred and mixed to obtain a spinning solution; finally, an anti-oxidative fiber mask containing Tremella fuciformis polysaccharide is obtained after electrostatic spinning and collection.

[0014] Preferably, the stirring speed is 80-120 rpm; the stirring time is 2-4 hours, and the temperature is 55-75°C.

[0015] Preferably, the electrospinning parameters are: the filament outlet is a No. 6 needle, the spinning voltage is 10-30 kV, the spinning pole distance is 12-18 cm, and the spinning rate is 0.1-0.5 mL / h.

[0016] Beneficial effects of the present invention: The antioxidant fiber mask containing Tremella fuciformis polysaccharide described in the present invention is prepared from Tremella fuciformis polysaccharide and small molecule collagen peptide according to a specific ratio. Tremella fuciformis polysaccharide, as an exogenous antioxidant nutrient of the body, can play an effect of scavenging free radicals. In addition, Tremella fuciformis polysaccharide has good biocompatibility and can be better attached to the mask. While providing the skin with the required nutrition while having anti-oxidation effect, it plays a positive regulatory role on the skin microecology. DETAILED DESCRIPTION

[0017] The following is a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0018] Example 1. The antioxidant fiber facial mask containing Tremella fuciformis polysaccharide of this embodiment comprises, by weight: 8 parts of Tremella fuciformis polysaccharide, 30 parts of small molecule collagen peptides, and 60 parts of deionized water.

[0019] The method for extracting Tremella fuciformis polysaccharide of the present embodiment comprises the following steps: S11. Pretreatment of Tremella fruiting bodies The Tremella fuciformis fruiting bodies were washed, dried to constant weight, crushed to pass through a 40-mesh sieve, added with 12 times the weight of 92% ethanol solution, soaked for 12 hours, and filtered to obtain filtrate A and precipitate A; 12 times the volume of 92% ethanol solution was added to precipitate A, soaked for 12 hours, and filtered again to obtain filtrate B and precipitate B, and precipitate B was naturally air-dried to constant weight to obtain an experimental sample.

[0020] S12, Enzyme-assisted Extraction of Tremella Polysaccharide The experimental sample was dissolved in 70 times the weight of deionized water, the temperature was controlled at 75°C, and the extraction was carried out for 0.5h. After cooling to 45°C, 7.02% of the complex enzyme was added, wherein the complex enzyme was composed of cellulase, papain and pectinase in a weight ratio of 1:1:0.65.

[0021] After 30 minutes of enzymatic hydrolysis, the centrifugal speed was set to 7000 r / min, the enzymatic hydrolyzate was centrifuged for 20 minutes, the supernatant was taken, anhydrous ethanol was added to the supernatant until the ethanol solution concentration was 75%, and alcohol precipitation was carried out at 4°C for 18 hours. Then, the mixture was centrifuged at 7000 r / min for 8 minutes, the precipitate was taken, and freeze-dried to obtain Tremella fuciformis polysaccharide.

[0022] The preparation method of the antioxidant fiber mask containing Tremella fuciformis polysaccharide of the present embodiment is: Tremella fuciformis polysaccharide, small molecule collagen peptide and water are mixed, stirred and mixed to obtain a spinning solution; finally, an antioxidant fiber mask containing Tremella fuciformis polysaccharide is obtained after electrostatic spinning and collection; the stirring speed is 100rmp; the stirring time is 3h, and the temperature is 60°C; the electrostatic spinning parameters are: the outlet is a No. 6 needle, the spinning voltage is 2kV, the spinning pole distance is 15m, and the spinning rate is 0.4L / h.

[0023] Example 2. The antioxidant fiber facial mask containing Tremella fuciformis polysaccharide of this embodiment comprises, by weight: 30 parts of Tremella fuciformis polysaccharide, 10 parts of small molecule collagen peptides, and 80 parts of deionized water.

[0024] The method for extracting Tremella fuciformis polysaccharide of the present embodiment comprises the following steps: S11. Pretreatment of Tremella fruiting bodies The Tremella fuciformis fruiting bodies were washed, dried to constant weight, crushed to pass through an 80-mesh sieve, added with 18 times the weight of 98% ethanol solution, soaked for 36 hours, and filtered to obtain filtrate A and precipitate A; 18 times the volume of 98% ethanol solution was added to precipitate A, soaked for 36 hours, and filtered again to obtain filtrate B and precipitate B, and precipitate B was naturally air-dried to constant weight to obtain an experimental sample.

[0025] S12, Enzyme-assisted Extraction of Tremella Polysaccharide The experimental sample was dissolved in 90 times the weight of deionized water, the temperature was controlled at 85°C, and the extraction was carried out for 1.5 hours. After cooling to 55°C, 7.12% of the complex enzyme was added, wherein the complex enzyme was composed of cellulase, papain and pectinase in a weight ratio of 1:1:0.85.

[0026] After 60 minutes of enzymatic hydrolysis, the centrifugal speed was set to 9000 r / min, the enzymatic hydrolyzate was centrifuged for 40 minutes, the supernatant was taken, anhydrous ethanol was added to the supernatant to a concentration of 80% of the ethanol solution, and the solution was precipitated at 4°C for 30 hours, and then centrifuged at 9000 r / min for 12 minutes. The precipitate was taken and freeze-dried to obtain Tremella fuciformis polysaccharide.

[0027] The preparation method of the antioxidant fiber mask containing Tremella fuciformis polysaccharide of the present embodiment is: Tremella fuciformis polysaccharide, small molecule collagen peptide and water are mixed, stirred and mixed to obtain a spinning solution; finally, an antioxidant fiber mask containing Tremella fuciformis polysaccharide is obtained after electrostatic spinning and collection; the stirring speed is 100rmp; the stirring time is 3h, and the temperature is 60°C; the electrostatic spinning parameters are: the outlet is a No. 6 needle, the spinning voltage is 2kV, the spinning pole distance is 15m, and the spinning rate is 0.4L / h.

[0028] Example 3. The antioxidant fiber facial mask containing Tremella fuciformis polysaccharide of this embodiment comprises, by weight: 10 parts of Tremella fuciformis polysaccharide, 15 parts of small molecule collagen peptides, and 75 parts of deionized water.

[0029] The method for extracting Tremella fuciformis polysaccharide of the present embodiment comprises the following steps: S11. Pretreatment of Tremella fruiting bodies The Tremella fuciformis fruiting bodies were washed, dried to constant weight, crushed and passed through a 60-mesh sieve, 15 times the weight of a 95% ethanol solution was added, the mixture was soaked for 24 hours, and filtered to obtain a filtrate A and a precipitate A; 15 times the volume of a 95% ethanol solution was added to the precipitate A, the mixture was soaked for 24 hours, and the mixture was filtered again to obtain a filtrate B and a precipitate B. The precipitate B was naturally air-dried to constant weight to obtain an experimental sample.

[0030] S12, Enzyme-assisted Extraction of Tremella Polysaccharide The experimental sample was dissolved in 70-90 times the weight of deionized water, the temperature was controlled at 80°C, extracted for 1 hour, cooled to 50°C, and then 7.07% of the complex enzyme was added, wherein the complex enzyme was composed of cellulase, papain and pectinase in a weight ratio of 1:1:0.75.

[0031] After 45 minutes of enzymatic hydrolysis, the centrifugal speed was set to 8000 r / min, the enzymatic hydrolyzate was centrifuged for 30 minutes, the supernatant was taken, anhydrous ethanol was added to the supernatant until the concentration of the ethanol solution was 80%, and the solution was precipitated at 4°C for 24 hours. The solution was then centrifuged at 8000 r / min for 10 minutes, the precipitate was taken, and freeze-dried to obtain Tremella fuciformis polysaccharide.

[0032] The preparation method of the antioxidant fiber mask containing Tremella fuciformis polysaccharide of the present embodiment is: Tremella fuciformis polysaccharide, small molecule collagen peptide and water are mixed, stirred and mixed to obtain a spinning solution; finally, an antioxidant fiber mask containing Tremella fuciformis polysaccharide is obtained after electrostatic spinning and collection; the stirring speed is 100rmp; the stirring time is 3h, and the temperature is 60°C; the electrostatic spinning parameters are: the outlet is a No. 6 needle, the spinning voltage is 2kV, the spinning pole distance is 15m, and the spinning rate is 0.4L / h.

[0033] Comparative Example 1. The difference from Example 3 is that Tremella fuciformis polysaccharide is not contained.

[0034] Comparative Example 2. The difference from Example 3 is that peach gum polysaccharide is used instead of Tremella fuciformis polysaccharide.

[0035] Comparative Example 3. The difference from Example 3 is that Tremella fuciformis polysaccharide replaces Tremella fuciformis polysaccharide.

[0036] The performance of the facial mask prepared by the present invention was tested and recorded as follows: 1.ABTS ＋ Determination of free radical scavenging ability ABTS ＋ The free radical scavenging ability is one of the important criteria for determining the antioxidant activity of polysaccharides. ＋ Comparison of free radical scavenging ability, testing the ABTS of this product ＋ The free radical scavenging ability, specific test results are shown in Table 1.

[0037] Table 1

[0038] 2. Skin elasticity test Skin elasticity is tested using the Skin Elasticity Tester MPA580. The test principle is based on the suction and stretching principles. A negative pressure is generated on the surface of the skin being tested to suck the skin into a specific test probe. The depth of the skin sucked into the test probe is measured by a non-contact optical test system.

[0039] The better the elasticity of the skin, the higher the Ue value and the lower the Uv value; the worse the elasticity of the skin, the lower the Ue value and the higher the Uv value. The test results are shown in Table 2.

[0040] Table 2

[0041] It can be seen from Table 1 and Table 2 that the components of the product of the present invention synergize and enhance the effect, have good antioxidant properties, and can provide the skin with the necessary nutrition, increase skin elasticity, and improve skin condition.

[0042] By comparing Examples 1-3 and Comparative Examples 1-3, it can be seen that the antioxidant fiber mask containing Tremella fuciformis polysaccharide described in the present invention, wherein the Tremella fuciformis polysaccharide has more superior antioxidant properties than peach gum polysaccharide and Tremella fuciformis polysaccharide. In the mask prepared under the various parameter conditions of the present invention, it can provide more nutrition to the skin, is more friendly to the skin, can effectively increase the elasticity of the skin, improve the skin microecology, and exert an antioxidant effect.

[0043] Inspired by the above-mentioned ideal embodiments of the present invention, it should be pointed out that the technical scope of the present invention is not limited to the contents of the specification. For ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. An antioxidant fiber mask containing Tremella fuciformis polysaccharide, characterized in that: The antioxidant fiber mask containing Tremella fuciformis polysaccharide comprises, by weight: 6 to 30 parts of Tremella fuciformis polysaccharide, 10 to 45 parts of small molecule collagen peptides, and 30 to 80 parts of deionized water.

2. The antioxidant fiber mask containing Tremella fuciformis polysaccharide according to claim 1, characterized in that: The antioxidant fiber facial mask containing Tremella fuciformis polysaccharide comprises, by weight: 8 to 15 parts of Tremella fuciformis polysaccharide, 12 to 30 parts of small molecule collagen peptides, and 60 to 80 parts of deionized water.

3. The antioxidant fiber mask containing Tremella fuciformis polysaccharide according to claim 1, characterized in that: The antioxidant fiber facial mask containing Tremella fuciformis polysaccharide comprises, by weight: 10 parts of Tremella fuciformis polysaccharide, 15 parts of small molecule collagen peptides, and 75 parts of deionized water.

4. The antioxidant fiber mask containing Tremella fuciformis polysaccharide according to claim 1, characterized in that: The method for extracting Tremella fuciformis polysaccharide comprises the following steps: S11. Pretreatment of Tremella fruiting bodies The Tremella fuciformis fruiting bodies were washed, dried to constant weight, crushed through a 40-80 mesh sieve, added with 12-18 times the weight of a 92-98% ethanol solution, soaked for 12-36 hours, and filtered to obtain a filtrate A and a precipitate A; 12-18 times the volume of a 92-98% ethanol solution was added to the precipitate A, soaked for 12-36 hours, and filtered again to obtain a filtrate B and a precipitate B, and the precipitate B was naturally air-dried to constant weight to obtain an experimental sample; S12, Enzyme-assisted Extraction of Tremella Polysaccharide The experimental sample is dissolved in 70-90 times the weight of deionized water, the temperature is controlled at 75-85°C, and the extraction is performed for 0.5-1.5h. After cooling to 45-55°C, 7.02-7.12% of the complex enzyme is added, wherein the complex enzyme is composed of cellulase, papain and pectinase in a weight ratio of 1:1: (0.65-0.85); After 30-60 minutes of enzymatic hydrolysis, the centrifugal speed is set to 7000-9000 r / min, the enzymatic hydrolyzate is centrifuged for 20-40 minutes, the supernatant is taken, anhydrous ethanol is added to the supernatant until the concentration of the ethanol solution is 60-80%, and alcohol precipitation is carried out at 4°C for 18-30 hours, and then centrifuged at a speed of 7000-9000 r / min for 8-12 minutes, the precipitate is taken, and freeze-dried to obtain Tremella fuciformis polysaccharide.

5. The antioxidant fiber mask containing Tremella fuciformis polysaccharide according to claim 4, characterized in that: The molecular weight of the Tremella fuciformis polysaccharide is 200-2000 kD.

6. The antioxidant fiber mask containing Tremella fuciformis polysaccharide according to claim 5, characterized in that: The molecular weight of the Tremella fuciformis polysaccharide is 500-800 kD.

7. The method for preparing an antioxidant fiber mask containing Tremella fuciformis polysaccharide according to any one of claims 1 to 6 is: Tremella fuciformis polysaccharide, small molecule collagen peptide and water are mixed, stirred and mixed to obtain a spinning solution; finally, an anti-oxidative fiber mask containing Tremella fuciformis polysaccharide is obtained after electrostatic spinning and collection.

8. The method for preparing the antioxidant fiber mask containing Tremella fuciformis polysaccharide according to claim 7, characterized in that: The stirring speed is 80-120 rpm; the stirring time is 2-4 hours, and the temperature is 55-75°C.

9. The method for preparing the antioxidant fiber mask containing Tremella fuciformis polysaccharide according to claim 5, characterized in that: The electrospinning parameters are as follows: the filament outlet is a No. 6 needle, the spinning voltage is 10-30 kV, the spinning pole distance is 12-18 cm, and the spinning rate is 0.1-0.5 mL / h.

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