Ginseng / purslane combined extract and application thereof

Abstract

The ginseng / purslane combined extract provided by the invention takes ginseng and heat-treated purslane as main raw materials, and the ginseng and the heat-treated purslane are extracted together, matched with each other and have a synergistic effect, so that active ingredients of the ginseng and the purslane are dissolved out to the maximum extent, and the effects of the ginseng and the purslane are fully exerted; the compound has obvious effects of improving the hypoxia endurance capacity of the body, promoting the metabolism and oxygen utilization rate of the body under the hypoxia condition, improving the anti-hypoxia capacity, resisting fatigue and recovering the mitochondrial function, can be used in daily life, and can be used in medicines, health-care products and food additives. The ginseng / purslane combined extract disclosed by the invention has the characteristics of simple preparation process, economy, safety, easiness in absorption and the like, and is suitable for industrial production.

Description

Technical Field

[0001] The invention belongs to the field of medical technology, and particularly relates to a ginseng / purslane combined extract and application thereof. Background Art

[0002] As society continues to progress, the pace of life and mental stress increase, and fatigue and other phenomena are common problems among people in "sub-healthy states" and are one of the important reasons for the decline in the body's motor ability and functional regulation. Oxygen is necessary for the existence of the current main life forms. Diseases caused by hypoxia and fatigue are common, such as heart failure, shock, chronic heart failure, and bronchiectasis. At the same time, common carbon monoxide poisoning, asphyxiation, anesthesia, and poisoning in life can also cause hypoxia or insufficient oxygen supply to the body. Even a short-term local lack of oxygen supply may cause irreversible damage to cells, tissues, and organs, causing some important physiological and pathological changes. How to improve human tolerance to hypoxia and effectively intervene in the damage of hypoxia to the human body are key issues that we must face and solve.

[0003] At present, the commonly used method to resist hypoxia is through pre-exercise, that is, pre-exposure to a tolerable hypoxic environment for a period of time, and then entering a hypoxic environment with a lower oxygen concentration after tolerance is developed. Although pre-exercise can enhance the body's adaptability to hypoxia to a certain extent, it is difficult for many people to implement due to the limitations of physical fitness, time, location and other conditions. Western medicine is mainly used to improve various symptoms caused by hypoxia, such as acetazolamide, which reduces the effective circulating blood volume and reduces the heart load through diuresis, thereby improving the ability to resist hypoxia, but long-term use will lead to more serious adverse reactions. Therefore, seeking effective ingredients from traditional Chinese medicine, using the modern "food supplement" concept, and applying medicinal materials with the same origin of medicine to the development of functional products is an inevitable trend in the current healthy development of traditional Chinese medicine, and it is also a need for the sustainable development of modernization.

[0004] Ginseng (Panax ginseng CAMey) is a traditional Chinese herbal medicine with outstanding effects in strengthening the body, improving immune function, resisting fatigue, improving intelligence, regulating the nervous system and blood circulation, etc. Its active ingredients include saponins, polysaccharides and amino acids. Purslane is a common wild vegetable in daily life, known as the "longevity vegetable". It has the effects of clearing away heat and dampness, detoxifying and reducing swelling, promoting diuresis and reducing swelling, and nourishing blood and beauty. Its anti-inflammatory and bactericidal effects are particularly prominent, and it is known as a natural antibiotic; it is also rich in protein, vitamin C, vitamin E and norepinephrine, which can promote the body to secrete insulin and regulate the body's sugar metabolism process. It is one of the medicinal and edible medicinal materials with high nutritional and medicinal value. The application of ginseng and many active ingredients in functional foods is a hot topic of current research. It can not only meet the needs of modern people's daily medical care, but also fully play a synergistic role with other medicinal and edible medicinal materials, enhance nutritional value, and promote the sustainable and healthy development of the ginseng industry. The two are organically combined to give full play to the synergistic effect of traditional Chinese medicine, promote the dissolution of effective ingredients, and play a unique pharmacological effect, improve the utilization efficiency of ginseng and purslane, and broaden its application range. At present, there are few reports on the research of synergistic compatibility of ginseng and purslane. CN1569127A discloses a purslane extract and ginsenoside compound preparation, which is prepared by reflux of petroleum ether and decoction of distilled water, and then made into ginsenoside compound preparation together with ginsenoside, which can be applied to the preparation of medicine and health food fields, but there is no report on the research of synergistic compatibility of ginseng and purslane. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a ginseng / purslane combined extract and its application in view of the deficiencies of the above-mentioned prior art. The present invention makes full use of the characteristics of the active ingredients in ginseng and purslane, extracts the two efficiently, promotes the effective dissolution of ginsenosides, amino acid glycosides, purslane polysaccharides and flavonoids, and maximizes the use efficiency of medicinal materials. It can effectively make up for the shortcomings of insufficient extraction of active ingredients, insignificant pharmacological effects and obvious side effects when ginseng or purslane is used alone, making the medicinal properties more mild, the active ingredients more prominent, and the nutritional and medicinal values ​​richer, and can be used in the fields of medicine or health products and food additives.

[0006] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0007] A ginseng / purslane combined extract is obtained by co-extracting ginseng and purslane raw materials with a solvent and then concentrating under reduced pressure. The weight parts of the ginseng and purslane raw materials are as follows: 3 to 12 parts of ginseng and 10 to 30 parts of purslane.

[0008] Preferably, the ginseng raw material includes 4-5 year old fresh ginseng, sun-dried ginseng or red ginseng.

[0009] Preferably, the purslane raw material is obtained by taking the aerial part of fresh purslane, washing and then heat treating it; the heat treatment method includes steaming or steaming with liquid auxiliary materials, the steaming time is 30 to 240 minutes, the steaming temperature is 90 to 120°C, after the steaming is completed, taking it out and drying it at 45 to 55°C for 24 to 48 hours; the liquid auxiliary material in the liquid auxiliary material steaming is any one of vinegar, rice wine, honey, rice water or ginger juice.

[0010] Preferably, the solvent is one or more of water, lower alcohol, lower ketone or ethyl acetate; the lower alcohol includes methanol, ethanol or n-butanol, and the lower ketone is acetone.

[0011] Preferably, the ratio of the ginseng and purslane raw materials to the solvent is 1:10 to 1:30;

[0012] Preferably, the co-extraction method comprises soaking extraction, reflux extraction, ultrasonic extraction, countercurrent extraction or supercritical extraction.

[0013] The ginseng / purslane combined extract of the present invention has ginsenoside content of 0.01-3.0%, amino acid glycoside content of 0.01-2.0%, purslane flavonoid content of 1-30.0%, and has the effects of resisting hypoxia, anti-fatigue and restoring mitochondrial function.

[0014] The present invention also provides an application of the ginseng / purslane combined extract in anti-hypoxia or anti-fatigue medicines, health products and food additives.

[0015] Preferably, the dosage forms of the anti-hypoxia or anti-fatigue drugs and health products include tablets, capsules, powder injections, injections, pills, soft capsules, granules or patches, etc. The ginseng / purslane combined extract of the present invention can also be mixed with other pharmaceutical carriers or food carriers, and the solid or liquid excipients used are well known in the art, for example: powders are powders for oral administration, and their excipients include lactose, starch, dextrin, calcium carbonate, synthetic or natural aluminum sulfate, magnesium oxide, magnesium stearate, sodium bicarbonate, dry yeast, etc.; the excipients of solutions include water, glycerol, 1,2-propylene glycol, monosaccharide syrup, ethanol, ethylene glycol, polyethylene glycol, sorbitol, etc.; the excipients of ointments can use hydrophobic agents or hydrophilic agents composed of fat oils, aqueous lanolin, vaseline, glycerol, beeswax, wood wax, liquid paraffin, etc.

[0016] The present invention has significant technical effects due to the adoption of the above technical solution:

[0017] 1. The present invention provides a ginseng / purslane combined extract, which takes ginseng and heat-treated purslane as main raw materials, and the two are extracted together, cooperate with each other, and synergistically enhance the effect, so as to dissolve the active ingredients of ginseng and purslane to the maximum extent, give full play to the efficacy of ginseng and purslane, and do not add any chemical components. It can effectively make up for the shortcomings of insufficient extraction of active ingredients, insignificant pharmacological effects, and the occurrence of adverse reactions such as dryness and violent effects when ginseng or purslane is used alone. The ginseng / purslane combined extract of the present invention has significant activity and mild medicinal properties, and can further improve the balance of the body.

[0018] 2. Through the analysis of the active ingredients and pharmacological effects of the ginseng / purslane combined extract, it can be seen that the active ingredient content of the ginseng / purslane combined extract is significantly improved, the enzymatic reaction is increased, the dissolution of amino acid glycoside active ingredients such as arginine disaccharide (AFG) in ginseng is promoted, and the dissolution rate of purslane flavonoid components is maximized. The ginseng / purslane combined extract of the present invention has a ginsenoside content of 0.01-3.0%, an amino acid glycoside content of 0.01-2.0%, and a purslane flavonoid content of 1-30.0%, which mainly has a significant effect on improving the body's hypoxia tolerance, promoting the body's metabolism and oxygen utilization under hypoxic conditions, improving anti-hypoxia ability, anti-fatigue and restoring mitochondrial function, and can be used in daily life, and can be used in medicines, health products and food additives.

[0019] 3. The ginseng / purslane combined extract of the present invention has the characteristics of simple preparation process, economy, safety and easy absorption, and is suitable for industrial production. It is a health preparation that can be used in anti-hypoxia, anti-fatigue drugs or functional foods, and can be taken for a long time. It broadens the use of dual-purpose medicinal materials for medicine and food, gives full play to the coordination effect of Chinese herbal medicine, and provides material reference for clinical medication.

[0020] The present invention is further described in detail below with reference to the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a liquid chromatography-mass spectrometry (LC / MS) diagram of the ginseng / purslane combined extract of the present invention. DETAILED DESCRIPTION

[0022] Example 1

[0023] This embodiment is a method for preparing a ginseng / purslane combined extract, specifically co-extracting ginseng and purslane, comprising the following steps:

[0024] Take the aerial part of fresh purslane, wash it, wrap it with gauze and place it in a vertical pressure steam sterilizer, use distilled water as a steam source, preheat it for 30 minutes, steam it under high pressure at 105°C for 60 minutes, then take it out and put it in an oven to dry at 45°C for 24 hours to obtain the heat-treated purslane; take 15g of the heat-treated purslane and 5g of 4-5 year old fresh ginseng, mix them, grind them, use 200g of 70% by volume ethanol aqueous solution for ultrasonic extraction 3 times, combine the extracts, and concentrate them under reduced pressure to obtain a ginseng / purslane combined extract.

[0025] Example 2

[0026] This embodiment is a method for preparing a ginseng / purslane combined extract, comprising the following steps:

[0027] Take the aerial part of fresh purslane, wash it, soak it in rice vinegar for 2 hours, put it in a steam cooker, use distilled water as a steam source, preheat it for 30 minutes, steam it at 90°C for 180 minutes, then take it out and put it in an oven to dry at 55°C for 24 hours to obtain the heat-treated purslane; take 30g of the heat-treated purslane and 12g of raw sun-dried ginseng, mix them, crush them, reflux extract them with 1260g of acetone for 3 times, combine the extracts, and concentrate them under reduced pressure to obtain a ginseng / purslane combined extract.

[0028] Example 3

[0029] This embodiment is a method for preparing a ginseng / purslane combined extract, comprising the following steps:

[0030] Take the aerial part of fresh purslane, wash it, soak it in rice wine for 2 hours, put it in a vertical pressure steam sterilizer, use distilled water as a steam source, preheat it for 30 minutes, steam it under high pressure at 90°C for 180 minutes, then take it out and put it in an oven for drying at 55°C for 30 hours to obtain the heat-treated purslane; take 25g of the heat-treated purslane and 10g of red ginseng, mix them, crush them, use 900g of ethyl acetate for supercritical extraction 3 times, combine the extracts, and concentrate them under reduced pressure to obtain a ginseng / purslane combined extract.

[0031] Example 4

[0032] This embodiment is a method for preparing a ginseng / purslane combined extract, comprising the following steps:

[0033] Take the aerial part of fresh purslane, wash it, soak it in rice water for 2 hours, put it in a vertical pressure steam sterilizer, use distilled water as a steam source, preheat it for 30 minutes, steam it under high pressure at 120°C for 90 minutes, then take it out and put it in an oven at 50°C for drying for 48 hours to obtain the heat-treated purslane; take 100g of the heat-treated purslane and 30g of red ginseng, mix them, crush them, soak and extract them with 2600g of n-butanol for 3 times, combine the extracts, and concentrate them under reduced pressure to obtain a ginseng / purslane combined extract.

[0034] Example 5

[0035] This embodiment is a method for preparing a ginseng / purslane combined extract, comprising the following steps:

[0036] Take the aerial part of fresh purslane, wash it, soak it in ginger juice for 2 hours, put it in a vertical pressure steam sterilizer, use distilled water as a steam source, preheat it for 30 minutes, steam it under high pressure at 100°C for 210 minutes, then take it out and put it in an oven at 50°C for drying for 36 hours to obtain the heat-treated purslane; take 20g of the heat-treated purslane and 8g of red ginseng, mix them, crush them, use 700g of 80% methanol aqueous solution with a volume fraction for countercurrent extraction 3 times, combine the extracts, and concentrate them under reduced pressure to obtain a ginseng / purslane combined extract.

[0037] Comparative Example 1

[0038] Purslane is extracted separately to prepare a purslane extract.

[0039] Take fresh purslane, wash it, wrap it with gauze and place it in a vertical pressure steam sterilizer. Put distilled water in the sterilizer as a steam source, preheat it for 30 minutes, steam it under high pressure at 105°C for 60 minutes, then take it out and put it in an oven at 45°C for drying for 24 hours to obtain the heat-treated purslane; take 15g of the heat-treated purslane, grind it, use 150g of 70% ethanol aqueous solution with a volume fraction of 70% for ultrasonic extraction 3 times, combine the extracts, and concentrate under reduced pressure to obtain the purslane extract.

[0040] Comparative Example 2

[0041] Ginseng is extracted separately to prepare a ginseng extract.

[0042] Take 5 g of fresh ginseng of 4 to 5 years old, wash it, crush it, and use 50 g of 70% ethanol aqueous solution with ultrasonic extraction for 3 times. Combine the extracts, concentrate them under reduced pressure, and obtain ginseng extract.

[0043] Example 6

[0044] This example is about the determination of effective ingredients of ginseng / purslane combined extract.

[0045] The ginseng / purslane combined extracts prepared in Examples 1 to 5, the purslane extracts prepared in Comparative Example 1, and the ginseng extracts prepared in Comparative Example 2 were subjected to the determination of the contents of different effective components, wherein the ginsenoside content was determined by high performance liquid chromatography (HPLC), the amino acid glycoside (AFG) content was determined by high performance liquid chromatography evaporative light scattering detector (HPLC-ELSD), and the purslane flavonoid content was determined by ultraviolet spectroscopy (UV). The results show that the ginsenoside content, amino acid glycoside (taking AFG as an example) and purslane flavonoid content in the ginseng / purslane combined extract are significantly higher than those in the single ginseng extract and the single purslane extract, and the ginsenoside content is increased by 60%, and it is rich in a variety of ginseng rare saponins, the AFG content is increased by 10%, and the purslane flavonoid components are increased by 65% ​​(as shown in Table 1). In addition, the purslane after heat treatment is more conducive to the dissolution of effective components, has a better taste effect, and is significantly less irritating. The results of Examples 2 to 5 show that the ginseng / purslane combined extract obtained by steaming and extracting after soaking in liquid auxiliary materials such as rice vinegar and rice wine has a significantly better content of active ingredients than purslane alone, and the extraction method has no obvious effect on the active ingredients. However, after steaming with liquid auxiliary materials, the effective ingredients of purslane are easier to dissolve, the taste of purslane is significantly enhanced, and the pharmacodynamic effect of the ginseng / purslane combined extract is also significantly improved.

[0046] Table 1 Content of active ingredients in ginseng / purslane combined extract

[0047] Group Ginsenosides(%) AFG(%) Purslane flavonoids (%) Total amount (%) Comparative Example 1 none none 16.18 16.18 Comparative Example 2 0.92 0.21 none 1.13 Example 1 1.48 0.25 25.90 27.63 Example 2 1.36 0.31 26.98 28.65 Example 3 1.52 0.27 27.86 29.65 Example 4 1.60 0.32 27.89 29.81 Example 5 1.54 0.26 28.21 30.01

[0048] The components of the ginseng / purslane combined extract obtained in Example 1 were analyzed by LC / MS, and the active ingredients were rich in the extract. Figure 1 Specific effective ingredients are shown in Table 2. The study found that the types and contents of ginsenosides increased when ginseng and purslane were extracted together, and the polysaccharides and proteins contained in purslane could effectively promote the transformation of ginsenosides, and rare saponin component groups such as ginsenoside Rg3, Rk1, Rg5 and Rh2 with high activity appeared, and small molecules such as AFG, the product of enzymatic reaction, were increased; on the contrary, trace elements such as amino acids, calcium and iron contained in ginseng could also promote the dissolution of purslane flavonoids and norepinephrine, making the nutritional and medicinal value of the two more abundant.

[0049] Table 2 LC / MS analysis of ginseng / purslane and their combined extracts

[0050]

[0051] Example 7

[0052] This example is an experiment on the anti-hypoxia tolerance of the ginseng / purslane combined extract.

[0053] After 1 week of adaptive feeding, male ICR mice were randomly divided into 5 groups, namely, a blank control group, a model group, a group administered with the ginseng / purslane combined extract prepared in Example 1, a group administered with the purslane extract prepared in Comparative Example 1, and a group administered with the ginseng extract prepared in Comparative Example 2. The administration groups were intragastrically administered with the ginseng / purslane combined extract once a day for 7 consecutive days, with a single administration dose of 400 mg kg -1 The blank control group and the model group were given equal amounts of saline by gavage for 7 consecutive days. On the 7th day, except for the blank control group, the mice in the model group and the three drug-treated groups were placed in a 150 mL inverted conical flask, the mouth of the flask was strictly sealed with the table, and a closed hypoxia model was constructed to observe the weight changes and survival time of the mice under normal pressure hypoxia. All mice were deprived of food but not water the night before the autopsy.

[0054] The results are shown in Table 3. After 7 days of administration, the weight of animals in each group increased, but there was no significant difference in weight between each administration group and the blank control group (P>0.05), indicating that each dose group had no effect on the weight of mice and was non-toxic. The study showed that the ginseng extract group alone and the ginseng / purslane combined extract group could prolong the survival time of mice under hypoxia to varying degrees, while the purslane extract group alone had no significant effect and died almost at the same time as the model group. It was also preliminarily proved that the ginseng / purslane combined extract group had better anti-hypoxia tolerance than the ginseng extract group alone (Table 3).

[0055] Table 3 Effects of ginseng / purslane and their combined extracts on body weight and survival time of mice ( n=8)

[0056] Group Weight at the beginning of the experiment (g) Body weight after 7 days of administration (g) Mouse survival time (min) Blank Group 24.9±1.6 29.8±1.7 —— Model Group 25.2±1.1 30.0±2.0 15.94±1.75 Comparative Example 1 25.4±1.3 29.9±1.7 16.03±1.54 Comparative Example 2 24.8±1.5 29.7±1.8 20.29±2.30 Example 1 24.7±1.5 30.2±1.6 <![CDATA[26.97±1.41 ** ]]>

[0057] Example 8

[0058] This example is about the effect of ginseng / purslane combined extract on mitochondrial function and tolerance of hypoxic mice.

[0059] In order to further explore the pharmacodynamic effects of the present invention on hypoxic mice, this example will comprehensively compare the effects of different extraction methods of the ginseng / purslane combination on mitochondrial function and tolerance function. Male ICR mice after 1 week of adaptive feeding were randomly divided into 9 groups, namely a blank control group, a model group, a group administered with the ginseng / purslane combination extract prepared in Examples 1 to 5, a group administered with the purslane extract prepared in Comparative Example 1, and a group administered with the ginseng extract prepared in Comparative Example 2. The administration group was intragastrically administered with the ginseng / purslane combination extract once a day for 7 consecutive days, with a single dose of 400 mL·kg -1The blank control group and the model group were given equal volumes of saline for continuous gavage for 7 days. On the 7th day, except for the blank control group, the other 8 groups of mice were placed in an oxygen-deficient environment. The oxygen-deficient environment was achieved by placing 10g of soda lime in a 500mL wide-mouth bottle to absorb carbon dioxide and water vapor. The eyeballs of the mice were removed and blood was collected 12 hours after the last gavage. The brain tissue of the mice was taken, washed, and frozen for later use. At the same time, the blood was collected and separated by a refrigerated centrifuge (12000r / min, 4℃, 10min, 2 times) to obtain serum, which was stored at -20℃. The effects of hypoxic conditions on mouse red blood cells, hematocrit, and hemoglobin were analyzed using a blood cell automatic analyzer.

[0060] After 7 days of administration, the weight of animals in each group increased, but there was no significant difference in weight between each administration group and the blank control group (P>0.05), indicating that each dose group had no effect on the weight of mice and was non-toxic. The study showed that the ginseng extract group (Comparative Example 2) and the ginseng / purslane combined extract group (Examples 1 to 5) could prolong the survival time of mice under hypoxia to varying degrees, while the purslane extract group (Comparative Example 1) had no obvious effect and died almost at the same time as the model group. This result was basically consistent with Example 7. Subsequently, the mitochondrial respiratory function of mice and the tolerance of mice were observed after 12 hours of hypoxia, and the results are shown in Table 4.

[0061] Table 4 Effects of ginseng / purslane combined extract on mitochondrial function and erythrocyte protein in hypoxic mice ( n=8)

[0062]

[0063]

[0064] Example 9

[0065] This example is an anti-fatigue experiment of the ginseng / purslane combined extract.

[0066] Based on the above clarification that the ginseng / purslane combined extract has a certain pharmacological effect on hypoxia capacity and mitochondrial function recovery, this example will further illustrate the scientific nature of the present invention through anti-fatigue experiments. This experiment will use Examples 1 to 5 and Comparative Examples 1 to 2 as test drugs, respectively, and use the swimming time of mice and the lactic acid concentration, blood urea nitrogen concentration and liver glycogen content in mice as indicators for evaluating the body's anti-fatigue, and examine the unique anti-fatigue effect of the ginseng / purslane combined extract on mice, so as to provide a scientific basis for developing it into a health food with the effect of alleviating fatigue in people in sub-healthy states.

[0067] After one week of adaptive feeding, male ICR mice were randomly divided into 9 groups, namely, a blank control group, a model group, Examples 1 to 5, and Comparative Examples 1 to 2. Two batches of experiments were conducted, and corresponding test drugs were administered by gavage. The model group was administered with saline at a dose of 400 mg kg -1 , gavage for 14 consecutive days, and the animals were free to eat and drink.

[0068] (1) Body mass measurement

[0069] The body weight of mice in each group was measured at the end of the experiment on the 7th day and the 14th day from the beginning of the experiment. The results are shown in Table 5:

[0070] Table 5 Changes in body weight of various mice before and after the first batch of experiments ( n=10)

[0071]

[0072]

[0073] As shown in Table 5, there was no significant difference among the groups in terms of initial body weight, intermediate body weight or final body weight of mice (P>0.05), indicating that the present invention had no effect on the body weight of mice.

[0074] (2) Weighted swimming test

[0075] 30 minutes after the last administration of the test drug in the first batch, except for the blank control group, the tail base of the mice in the other groups was loaded with a weight of 5% of body weight. The mice were placed in a swimming box with a water depth of 30 cm and a water temperature of (25±0.5)℃, and the time from the start of swimming to the time when the head completely sank into the water and could not float to the surface (death) was recorded as its loaded swimming time. The results are shown in Table 6.

[0076] (3) Blood lactate, serum urea nitrogen and liver glycogen determination

[0077] 30 minutes after the last administration of the test drug in the second batch, 20 μL of blood was collected from the orbital vein of the mice to measure the lactate concentration before swimming. Then the mice were placed in a swimming box with a water temperature of 30°C and a water depth of 35 cm for 90 minutes, and the eyeballs were immediately removed for blood collection. 20 μL was taken to measure the blood lactate level after swimming. The remaining blood sample was placed in a 4°C refrigerator for 3 hours and centrifuged at 3500r / min for 10 minutes; after separating the serum, the serum urea nitrogen was determined by fully automatic biochemical analysis. The animals were killed immediately after blood was collected, the liver was rinsed with physiological saline and dried with filter paper, 100 mg of the liver was accurately weighed, and the liver glycogen level was determined using a kit (anthrone method). The results are shown in Table 6.

[0078] Table 6 Effects of ginseng / purslane combined extract on anti-fatigue of mice ( n=8)

[0079]

[0080]

[0081] Note: Compared with the model group, *P<0.05, **P<0.01, ***P<0.001.

[0082] According to the comparison of weighted swimming time in Table 6, the ginseng / purslane combined extracts (Examples 1-5) prepared by different methods can prolong the weighted swimming time of mice to varying degrees, which is significantly better than the ginseng and purslane separate extraction groups (Comparative Examples 1-2). At the same time, by measuring the blood lactate concentration, serum urea nitrogen and liver glycogen content in mice after weighted exercise, it can be seen that compared with the blank control group, the blood lactate level and urea nitrogen content increased significantly, while the test groups of Examples 1-5 can inhibit the increase of their content to varying degrees, and the difference between different extraction methods is small; from the perspective of glycogen content, the test group of the present invention can maintain the glycogen content well, and is closer to the blank control group (P<0.001), indicating that the ginseng / purslane combined extract has a significant anti-fatigue effect.

[0083] The above experimental data fully illustrate that through the analysis of the active ingredients and pharmacological effects of the ginseng / purslane combined extract, it can be seen that the ginseng / purslane combined extraction is significantly better than the extraction of ginseng and purslane alone, and its active ingredient content is significantly improved, and the enzymatic reaction is increased, promoting the dissolution of amino acid glycoside active ingredients such as arginine disaccharide (AFG) in ginseng, and maximizing the dissolution rate of purslane flavonoids. The ginseng / purslane combined extract of the present invention has a ginsenoside content of 0.01-3.0%, an amino acid glycoside content of 0.01-2.0%, and a purslane flavonoid content of 1-30.0%. The pharmacodynamics experiment shows that the pharmacological effect of the ginseng / purslane combined extract is significantly higher than that of the single extraction. At the same time, the single purslane extract has no anti-hypoxia activity and anti-fatigue effect. After adding ginseng, it can be significantly better than the single ginseng treatment group, which fully demonstrates that the ginseng / purslane combined extract of the present invention has good anti-hypoxia ability and restores mitochondrial function, and can be made into effective anti-hypoxia or anti-fatigue drugs, health products and food additives, etc., effectively making up for the complex separation technology and single active ingredients, which limit its application and development. The present invention has the characteristics of economy, convenient operation, easier digestion and absorption by the body, etc., broadens the use of medicinal and edible dual-purpose medicinal materials, gives full play to the coordination effect of Chinese herbal medicine, and provides material reference for clinical medication.

[0084] The above is only a preferred embodiment of the present invention and does not limit the present invention in any way. Any simple modification, change and equivalent change made to the above embodiment according to the technical essence of the invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A ginseng / purslane combined extract, characterized in that: The ginseng / purslane combined extract is obtained by co-extracting ginseng and purslane raw materials with a solvent and then concentrating under reduced pressure. The weight proportions of the ginseng and purslane raw materials are as follows: 3 to 12 parts of ginseng and 10 to 30 parts of purslane.

2. The ginseng / purslane combined extract according to claim 1, characterized in that: The ginseng raw materials include 4-5 year old fresh ginseng, sun-dried ginseng or red ginseng.

3. The ginseng / purslane combined extract according to claim 1, characterized in that: The purslane raw material is obtained by taking the aerial part of fresh purslane, washing it and then subjecting it to heat treatment; the heat treatment method comprises steaming or steaming with liquid auxiliary materials, the steaming time is 30 to 240 minutes, the steaming temperature is 90 to 120° C. After the steaming is completed, the raw material is taken out and dried at 45 to 55° C. for 24 to 48 hours; the liquid auxiliary material in the liquid auxiliary material steaming is any one of vinegar, rice wine, honey, rice water or ginger juice.

4. The ginseng / purslane combined extract according to claim 1, characterized in that: The solvent is one or more of water, lower alcohol, lower ketone or ethyl acetate; the lower alcohol includes methanol, ethanol or n-butanol, and the lower ketone is acetone.

5. The ginseng / purslane combined extract according to claim 1, characterized in that: The ratio of the ginseng and purslane raw materials to the solvent is 1:10 to 1:

30.

6. The ginseng / purslane combined extract according to claim 1, characterized in that: The co-extraction method includes soaking extraction, reflux extraction, ultrasonic extraction, countercurrent extraction or supercritical extraction.

7. The ginseng / purslane combined extract according to claim 1, characterized in that: The ginseng / purslane combined extract has a ginsenoside content of 0.01-3.0%, an amino acid glycoside content of 0.01-2.0%, and a purslane flavonoid content of 1-30.0%.

8. Use of the ginseng / purslane combined extract according to any one of claims 1 to 7 in anti-hypoxia or anti-fatigue drugs, health products and food additives.

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