Biopharmaceutical stem cell reaction system and method for cell biological genetic engineering

Abstract

The invention discloses a biopharmacy stem cell reaction system and method for cell biological genetic engineering.The biopharmacy stem cell reaction system for the cell biological genetic engineering.The biopharmacy stem cell reaction system for the cell biological genetic engineeringcomprises a bottom plate, a mounting base is mounted on the bottom plate, a swing mechanism is arranged in the mounting base, and two fixing frames are fixedly connected to the bottom plate; a track plate is arranged on the fixing frames, a reaction tank is mounted on the swinging mechanism, an oscillating mechanism is arranged between the reaction tank and the swinging mechanism, the oscillating mechanism is movably connected with the track plate, a cleaning mechanism is arranged in the reaction tank, a transmission mechanism is arranged on the reaction tank, and a driving mechanism is arranged on one of the fixing frames. The driving mechanism is movably connected with the transmission mechanism and the swinging mechanism.

Description

Technical Field

[0001] The present invention relates to the field of genetic engineering technology, in particular to a biopharmaceutical stem cell reaction system and method for cell biological genetic engineering. Background Art

[0002] Stem cells are a type of multipotent cells with the ability to self-replicate. Under certain conditions, they can differentiate into a variety of functional cells. Stem cells are divided into embryonic stem cells and adult stem cells according to their developmental stage. They have broad prospects in the fields of regenerative medicine, biopharmaceuticals, and drug screening. When stem cells are cultured in vitro, stem cells are rapidly proliferated in a reaction device. In order to ensure the uniformity of the culture medium, the culture medium needs to be stirred to ensure that the stem cells can divide quickly and avoid local unevenness. However, due to the existing reaction device, stirring is performed by using a stirring shaft. However, since stem cells are animal cells, there is only a thin layer of cell membrane on the outer surface. When they come into contact with the stirring shaft, the stirring shaft is a rigid object, which will cause the cell membrane of the stem cells to appear. The damage leads to the death of stem cells, affects the number of stem cells, and is not conducive to the rapid proliferation of stem cells. To solve the above problems, a technical solution is disclosed in Patent No.: CN202210660001.3, but this technical solution still has some problems when used. When this technical solution is used, when the cells need to be mixed during the reaction, it is still necessary to use a stirring part to stir the mixed liquid, so it may still cause cell wall breakage during mixing, thereby affecting the number of stem cells, and during the mixing process, the cells are easy to adhere to the side wall of the reaction tank, which not only affects the mixing efficiency but also affects the number of cells discharged, thereby affecting the practicality and ease of use of the equipment.

[0003] Based on this, the present invention designs a biopharmaceutical stem cell reaction system and method for cell biological genetic engineering to solve the above problems. Summary of the invention

[0004] The purpose of the present invention is to provide a biopharmaceutical stem cell reaction system and method for cell biological genetic engineering to solve the problem that the cell wall of the device proposed in the above background technology is prone to rupture during use, thereby affecting the number of cells.

[0005] To achieve the above object, the present invention provides the following technical solutions: A biopharmaceutical stem cell reaction system and method for cell biological genetic engineering, comprising a base plate, a mounting seat installed on the base plate, a swing mechanism arranged in the mounting seat, two fixing frames fixedly connected to the base plate, a track plate arranged on the fixing frames, a reaction tank installed on the swing mechanism, an oscillation mechanism arranged between the reaction tank and the oscillation mechanism, the oscillation mechanism and the track plate movably connected, a cleaning mechanism arranged in the reaction tank, a transmission mechanism arranged on the reaction tank, a driving mechanism arranged on one of the fixing frames, and the driving mechanism movably connected to the transmission mechanism and the oscillation mechanism respectively.

[0006] As a further solution of the present invention, the rocking mechanism includes a rack, a limit groove, a first limit rod, a first rotating column and a first gear. The mounting seat is slidably connected with a rack, limit grooves are provided on both sides of the rack, a first limit rod is provided between the limit groove and the mounting seat, the mounting seat is rotatably connected with a first rotating column, the first rotating column is installed with a first gear, and the first gear is meshingly connected with the rack.

[0007] As a further solution of the present invention, the oscillation mechanism includes a spring, a second limiting rod, a connecting rod and an auxiliary wheel. Two springs are fixedly connected to the reaction tank, one end of the spring is fixedly connected to the rocking mechanism, a second limiting rod is provided in the spring, a connecting rod is fixedly connected to the lower side of the reaction tank, two auxiliary wheels are provided on the connecting rod, and the auxiliary wheels are rollingly connected to the track plate.

[0008] As a further solution of the present invention, the cleaning mechanism includes a guide rod, a threaded rod, a movable frame and a cleaning plate. The reaction tank is provided with a guide rod and a threaded rod. The threaded rod is rotatably connected in the reaction tank. A movable frame is provided between the threaded rod and the guide rod. A cleaning plate is installed on the lower side of the movable frame. The cleaning plate is in a truncated cone shape. The cleaning plate is movably connected to the inner wall of the reaction tank.

[0009] As a further solution of the present invention, the transmission mechanism includes a speed regulating box, a transmission rod, a torsion spring, a second gear and a half gear. The reaction tank is installed with a speed regulating box, the speed regulating box is rotatably connected to the transmission rod, the transmission rod is sleeved with a torsion spring, the transmission rod is transmission-connected to the cleaning mechanism, the speed regulating box is provided with a second gear, one of the fixed frames is provided with a half gear, and the half gear is meshingly connected with the second gear.

[0010] As a further solution of the present invention, the driving mechanism includes a second rotating column, a third rotating column, a driving motor and a telescopic rod. The second rotating column and the third rotating column are rotatably connected to the fixed frame. The second rotating column is transmission-connected to the swing mechanism, and the third rotating column is transmission-connected to the transmission mechanism. The driving motor is installed on the fixed frame, and the output end of the driving motor is fixedly connected to the telescopic rod, and the telescopic rod is movably engaged with the second rotating column and the third rotating column, respectively.

[0011] As a further solution of the present invention, a baffle is fixedly connected inside the reaction tank, a silicone layer is provided on the surface of the baffle, a plurality of through holes are opened on the baffle, and a plurality of silicone bumps are installed on the baffle.

[0012] A biopharmaceutical stem cell reaction method for cell biological genetic engineering, the specific steps of the reaction method are as follows: Step 1: Place the material to be reacted in the reaction tank, then control the driving mechanism and the swing mechanism to engage, and then control the driving mechanism to work; Step 2: When the driving mechanism is working, the swing mechanism controls the reaction tank installed on its upper side to swing left and right, and the materials in the reaction tank are mixed during the swinging process; Step 3: When the reaction tank is shaking, the oscillating mechanism moves on the track plate to keep the reaction tank oscillating, thereby accelerating its mixing efficiency and ensuring its mixing quality; Step 4: After the equipment has mixed for a period of time, the reaction tank stops swinging. At this time, the driving mechanism controls the cleaning mechanism through the transmission mechanism to work, and then the materials attached to the inner wall of the reaction tank are cleaned into the mixed liquid, so as to continue mixing to ensure the quality of the mixing, and finally the reacted materials are discharged from the reaction tank.

[0013] Compared with the prior art, the present invention has the following beneficial effects: The present invention places the material that needs to react in the reaction tank, and then controls the driving mechanism and the swinging mechanism to engage in the machine. When the driving mechanism controls the swinging mechanism to work, the reaction tank swings left and right under the drive of the swinging mechanism. At the same time, during the swinging process, the material in the reaction tank will rock left and right, thereby mixing the material. The stirring of the stirring part of the device is reduced during mixing, thereby preventing the cells from being broken during the reaction. When the reaction tank is swinging, the material in the reaction tank can pass through the through holes and protrusions on the baffle plate, thereby increasing the mixing efficiency. When the reaction tank is swinging, the oscillation mechanism on the lower side of the reaction tank passes through the track of the track plate, so that the reaction tank passes through the spring in the oscillation mechanism to maintain the vibration of the reaction tank when it moves, thereby making the reaction tank more efficient during the reaction and mixing more efficient. At the same time, the cell breakage is reduced, the quality of the cell reaction is guaranteed, and the practicability and ease of use of the device are further improved.

[0014] After the equipment has been reacting for a period of time, the material in the reaction tank begins to adhere to the inner wall of the reaction tank, which in turn affects the quality and quantity of the reaction. At this time, the reaction tank is stopped from being swung, and the driving mechanism and the transmission mechanism are controlled to be connected for transmission. Then the driving mechanism controls the cleaning mechanism through the transmission mechanism to work, and then the cleaning mechanism moves from top to bottom. During the movement of the cleaning mechanism from top to bottom, the cleaning mechanism can effectively clean the material attached to the side wall of the reaction tank and make it enter the liquid in the reaction tank. Then the equipment is continued to be controlled to swing and mix, and repeated work is performed to effectively ensure the mixing quality of the equipment and reduce the adhesion of the liquid, thereby improving the practicability and efficiency of the equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for describing the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0016] Figure 1 This is a schematic diagram of the structure of the present invention from a front side perspective; Figure 2 This is a schematic diagram of the structure of the present invention from a rear side perspective; Figure 3 It is a schematic diagram of the cross-sectional structure of the present invention from a partial rear side perspective; Figure 4 It is a schematic diagram of the structure of the present invention from a local bottom side perspective; Figure 5 This is a schematic diagram of the partial front side cross-sectional structure of the present invention. Figure 6 The present invention is a flow chart of the reaction method.

[0017] In the accompanying drawings, the components represented by the reference numerals are listed as follows: 1. Base plate; 2. Mounting seat; 3. Swing mechanism; 4. Rack; 5. Limiting groove; 6. First limiting rod; 7. First rotating column; 8. First gear; 9. Fixed frame; 10. Track plate; 11. Reactor; 12. Oscillation mechanism; 13. Spring; 14. Second limiting rod; 15. Connecting rod; 16. Auxiliary wheel; 17. Cleaning mechanism; 18. Guide rod; 19. Threaded rod; 20. Moving frame; 21. Cleaning plate; 22. Transmission mechanism; 23. Speed ​​regulating box; 24. Transmission rod; 25. Torsion spring; 26. Second gear; 27. Half gear; 28. Driving mechanism; 29. ​​Second rotating column; 30. Third rotating column; 31. Driving motor; 32. Telescopic rod; 33. Baffle; 34. Through hole; 35. Bump. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] See also Figure 1-Figure 6 , the present invention provides a technical solution: A biopharmaceutical stem cell reaction system and method for cell biological genetic engineering, comprising a bottom plate, a mounting seat installed on the bottom plate, a swing mechanism inside the mounting seat, two fixed frames fixedly connected to the bottom plate, a track plate on the fixed frame, a reaction tank installed on the swing mechanism, an oscillation mechanism between the reaction tank and the oscillation mechanism, the oscillation mechanism and the track plate are movably connected, a cleaning mechanism is provided in the reaction tank, a transmission mechanism is provided on the reaction tank, a driving mechanism is provided on one of the fixed frames, and the driving mechanism is movably connected to the transmission mechanism and the oscillation mechanism respectively; During operation, the material to be reacted is placed in the reaction tank, and then the driving mechanism and the swinging mechanism are controlled to engage in the machine. When the driving mechanism controls the swinging mechanism to work, the reaction tank swings left and right under the drive of the swinging mechanism. At the same time, during the swinging process, the material in the reaction tank will swing left and right, thereby mixing the material. This is to reduce the stirring of the stirring part of the equipment during mixing, thereby preventing the cells from being broken during the reaction. When the reaction tank is swinging, the material in the reaction tank can pass through the through holes and bumps on the baffle plate, thereby increasing the mixing efficiency. When the reaction tank is swinging, the oscillation mechanism on the lower side of the reaction tank passes through the track of the track plate, so that the reaction tank passes through the spring in the oscillation mechanism to maintain the vibration of the reaction tank when it moves, thereby making the reaction tank more efficient during the reaction and mixing more efficient, while reducing The cell disruption ensures the quality of the cell reaction and further improves the practicability and ease of use of the equipment. After the equipment has been reacting for a period of time, the material in the reaction tank appears to adhere to the inner wall of the reaction tank, which in turn affects the quality and quantity of the reaction. At this time, the reaction tank is stopped from swinging, and the driving mechanism and the transmission mechanism are controlled to be connected for transmission. Then the driving mechanism controls the cleaning mechanism through the transmission mechanism to work, and then the cleaning mechanism moves from top to bottom. During the movement of the cleaning mechanism from top to bottom, the cleaning mechanism can effectively clean the material attached to the side wall of the reaction tank and make it enter the liquid in the reaction tank. Then the equipment is continued to be controlled to swing and mix, and repeated work is performed to effectively ensure the mixing quality of the equipment and reduce the adhesion of the liquid, thereby improving the practicability and efficiency of the equipment.

[0020] As a further solution of the present invention, the swing mechanism includes a rack, a limit groove, a first limit rod, a first rotating column and a first gear, the mounting seat is slidably connected with the rack, both sides of the rack are provided with limit grooves, a first limit rod is provided between the limit groove and the mounting seat, the mounting seat is rotatably connected with the first rotating column, the first gear is installed on the first rotating column, and the first gear is meshed with the rack; During operation, the control driving mechanism and the first rotating column are engaged, and the first rotating column controls the first gear to rotate. When the first gear rotates, the first gear drives the rack to move in the limiting groove under the limitation of the first limiting rod, so that the rack swings left and right. When the rack swings, the reaction tank can be effectively controlled to swing left and right, and then the materials in the reaction tank are oscillated and mixed, thereby effectively reducing the contact between the material and the stirring part and reducing cell breakage.

[0021] As a further solution of the present invention, the oscillation mechanism includes a spring, a second limiting rod, a connecting rod and an auxiliary wheel. Two springs are fixedly connected to the reaction tank, one end of the spring is fixedly connected to the swing mechanism, a second limiting rod is arranged inside the spring, a connecting rod is fixedly connected to the lower side of the reaction tank, two auxiliary wheels are arranged on the connecting rod, and the auxiliary wheels are rollingly connected to the track plate; During operation, when the reaction tank swings left and right, the auxiliary wheels move in the track plate, and the auxiliary wheels follow the track of the track plate to move. At the same time, when the auxiliary wheels move up and down following the track of the track plate, the length of the spring is simultaneously expanded and contracted, and then the reaction tank vibrates during the swinging process, thereby further improving the reaction efficiency and quality of the reaction tank.

[0022] As a further solution of the present invention, the cleaning mechanism includes a guide rod, a threaded rod, a movable frame and a cleaning plate. The guide rod and the threaded rod are arranged in the reaction tank. The threaded rod is rotatably connected in the reaction tank. A movable frame is arranged between the threaded rod and the guide rod. A cleaning plate is installed on the lower side of the movable frame. The cleaning plate is in a truncated cone shape. The cleaning plate is movably connected to the inner wall of the reaction tank. During operation, when the reaction tank stops swinging, the driving mechanism controls the rotation of the threaded rod through the transmission mechanism. At this time, the movable rack threadedly connected to the threaded rod moves under the control of the guide rod. When the movable rack moves downward, the cleaning plate on the movable rack can effectively clean the cells adhering to the side wall of the reaction tank into the active liquid inside the reaction tank, thereby further ensuring the reaction quality and reaction quantity of the cells, and further improving the practicality and ease of use of the equipment.

[0023] As a further solution of the present invention, the transmission mechanism includes a speed regulating box, a transmission rod, a torsion spring, a second gear and a half gear. The speed regulating box is installed on the reaction tank, the speed regulating box is rotatably connected to the transmission rod, the transmission rod is sleeved with a torsion spring, the transmission rod is transmission-connected to the cleaning mechanism, the speed regulating box is provided with a second gear, one of the fixed frames is provided with a half gear, and the half gear is meshingly connected with the second gear; During operation, when the driving mechanism controls the half gear to rotate, the half gear drives the second gear to rotate. At this time, the second gear controls the transmission rod to rotate through the speed regulating box. When the transmission rod rotates, the transmission rod controls the torsion spring to be compressed. At the same time, the transmission rod controls the threaded rod in the cleaning mechanism to rotate, so that the cleaning mechanism cleans the side wall of the reaction tank. When the half gear and the second gear are disengaged, the torsion spring is reset, and then the cleaning mechanism is reset. In this way, the work is repeated, which can effectively ensure the cleanliness of the inner wall of the reaction tank and prevent cells from being attached to the inner wall of the reaction tank.

[0024] As a further solution of the present invention, the driving mechanism includes a second rotating column, a third rotating column, a driving motor and a telescopic rod, the second rotating column and the third rotating column are rotatably connected to the fixed frame, the second rotating column is transmission-connected to the swing mechanism, the third rotating column is transmission-connected to the transmission mechanism, the driving motor is installed on the fixed frame, the output end of the driving motor is fixedly connected to the telescopic rod, and the telescopic rod is movably connected to the second rotating column and the third rotating column respectively; During operation, when the telescopic rod is shortened, the telescopic rod controls the second rotating column to rotate. At this time, the second rotating column controls the swing mechanism to swing, thereby ensuring that the materials inside the reaction tank are mixed. At the same time, when the telescopic rod is extended, the telescopic rod and the third rotating column are engaged. At this time, the third rotating column drives the transmission mechanism to work, and then the cleaning mechanism cleans the inner wall of the reaction tank, thereby effectively ensuring the cleanliness of the inner wall.

[0025] As a further solution of the present invention, a baffle is fixedly connected inside the reaction tank, a silicone layer is provided on the surface of the baffle, a plurality of through holes are opened on the baffle, and a plurality of silicone bumps are installed on the baffle; During operation, when the material in the reaction tank is shaken, the material will flow out through the through holes on the baffle. At the same time, the protrusions can make the material have greater resistance during mixing and make the mixing efficiency higher, further improving the practicability of the equipment and the mixing effect.

[0026] A biopharmaceutical stem cell reaction method for cell biological genetic engineering, the specific steps of the reaction method are as follows: Step 1: Place the material to be reacted in the reaction tank, then control the driving mechanism and the swing mechanism to engage, and then control the driving mechanism to work; Step 2: When the driving mechanism is working, the swing mechanism controls the reaction tank installed on its upper side to swing left and right, and the materials in the reaction tank are mixed during the swinging process; Step 3: When the reaction tank is shaking, the oscillating mechanism moves on the track plate to keep the reaction tank oscillating, thereby accelerating its mixing efficiency and ensuring its mixing quality; Step 4: After the equipment has mixed for a period of time, the reaction tank stops swinging. At this time, the driving mechanism controls the cleaning mechanism through the transmission mechanism to work, and then the materials attached to the inner wall of the reaction tank are cleaned into the mixed liquid, so as to continue mixing to ensure the quality of the mixing, and finally the reacted materials are discharged from the reaction tank.

Claims

1. A biopharmaceutical stem cell reaction system for cell biological genetic engineering, comprising a base plate (1), on which a mounting seat (2) is mounted, characterized in that: The mounting seat (2) is provided with a swing mechanism (3), two fixing frames (9) are fixedly connected to the bottom plate (1), a track plate (10) is provided on the fixing frames (9), a reaction tank (11) is installed on the swing mechanism (3), an oscillating mechanism (12) is provided between the reaction tank (11) and the swing mechanism (3), the oscillating mechanism (12) and the track plate (10) are movably connected, a cleaning mechanism (17) is provided in the reaction tank (11), a transmission mechanism (22) is provided on the reaction tank (11), a driving mechanism (28) is provided on one of the fixing frames (9), and the driving mechanism (28) is movably connected to the transmission mechanism (22) and the swing mechanism (3), respectively.

2. The biopharmaceutical stem cell reaction system for cell biological genetic engineering according to claim 1, characterized in that: The rocking mechanism (3) comprises a rack (4), a limiting groove (5), a first limiting rod (6), a first rotating column (7) and a first gear (8); the mounting seat (2) is slidably connected with the rack (4); limiting grooves (5) are provided on both sides of the rack (4); a first limiting rod (6) is provided between the limiting groove (5) and the mounting seat (2); the mounting seat (2) is rotatably connected with the first rotating column (7); the first gear (8) is mounted on the first rotating column (7); and the first gear (8) and the rack (4) are meshingly connected.

3. The biopharmaceutical stem cell reaction system for cell biological genetic engineering according to claim 1, characterized in that: The oscillating mechanism (12) comprises a spring (13), a second limiting rod (14), a connecting rod (15) and an auxiliary wheel (16); the reaction tank (11) is fixedly connected to two springs (13); one end of the spring (13) is fixedly connected to the oscillating mechanism (3); a second limiting rod (14) is arranged inside the spring (13); a connecting rod (15) is fixedly connected to the lower side of the reaction tank (11); two auxiliary wheels (16) are arranged on the connecting rod (15); and the auxiliary wheels (16) are rollingly connected to the track plate (10).

4. The biopharmaceutical stem cell reaction system for cell biological genetic engineering according to claim 1, characterized in that: The cleaning mechanism (17) comprises a guide rod (18), a threaded rod (19), a movable frame (20) and a cleaning plate (21). The reaction tank (11) is provided with the guide rod (18) and the threaded rod (19). The threaded rod (19) is rotatably connected in the reaction tank (11). The movable frame (20) is provided between the threaded rod (19) and the guide rod (18). The cleaning plate (21) is mounted on the lower side of the movable frame (20). The cleaning plate (21) is in a truncated cone shape. The cleaning plate (21) is movably connected to the inner wall of the reaction tank (11).

5. The biopharmaceutical stem cell reaction system for cell biological genetic engineering according to claim 1, characterized in that: The transmission mechanism (22) comprises a speed regulating box (23), a transmission rod (24), a torsion spring (25), a second gear (26) and a half gear (27). The reaction tank (11) is mounted with the speed regulating box (23). The speed regulating box (23) is rotatably connected to the transmission rod (24). The transmission rod (24) is sleeved with a torsion spring (25). The transmission rod (24) is transmission-connected to the cleaning mechanism (17). The speed regulating box (23) is provided with a second gear (26). One of the fixing frames (9) is provided with a half gear (27). The half gear (27) is meshingly connected to the second gear (26).

6. The biopharmaceutical stem cell reaction system for cell biological genetic engineering according to claim 1, characterized in that: The driving mechanism (28) comprises a second rotating column (29), a third rotating column (30), a driving motor (31) and a telescopic rod (32); the second rotating column (29) and the third rotating column (30) are rotatably connected to the fixed frame (9); the second rotating column (29) is transmission-connected to the swing mechanism (3); the third rotating column (30) is transmission-connected to the transmission mechanism (22); the driving motor (31) is mounted on the fixed frame (9); the output end of the driving motor (31) is fixedly connected to the telescopic rod (32); the telescopic rod (32) is movably engaged with the second rotating column (29) and the third rotating column (30), respectively.

7. The biopharmaceutical stem cell reaction system for cell biological genetic engineering according to claim 1, characterized in that: A baffle (33) is fixedly connected inside the reaction tank (11), a silicone layer is provided on the surface of the baffle (33), a plurality of through holes (34) are opened on the baffle (33), and a plurality of silicone bumps (35) are installed on the baffle (33).

8. A biopharmaceutical stem cell reaction method for cell biological genetic engineering, applicable to the biopharmaceutical stem cell reaction system for cell biological genetic engineering according to any one of claims 1 to 7, characterized in that: The specific steps of this reaction method are as follows: Step 1: placing the material to be reacted in the reaction tank (11), then controlling the driving mechanism (28) and the swing mechanism (3) to engage, and then controlling the driving mechanism (28) to operate; Step 2: When the driving mechanism (28) is working, the swing mechanism (3) controls the reaction tank (11) installed on its upper side to swing left and right, and the materials in the reaction tank (11) are mixed during the swinging process; Step 3: while the reaction tank (11) is being shaken, the shaking mechanism (12) moves on the track plate (10), thereby maintaining the shaking of the reaction tank (11), thereby accelerating its mixing efficiency and ensuring its mixing quality; Step 4: After the equipment has mixed for a period of time, the reaction tank (11) stops swinging. At this time, the driving mechanism (28) controls the cleaning mechanism (17) to work through the transmission mechanism (22), and then the materials attached to the inner wall of the reaction tank (11) are cleaned into the mixed liquid, so that the mixing continues to ensure the quality of the mixing. Finally, the reacted materials are discharged from the reaction tank (11).

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