Culture medium for endophytic growth-promoting bacteria of tree root system and preparation method of culture medium

Abstract

The invention provides a culture medium for endophytic growth-promoting bacteria of a tree root system and a preparation method of the culture medium, and belongs to the technical field of biological agents. According to the culture medium disclosed by the invention, through the synergistic effect of all the components, required nutritional ingredients can be provided so as to support and enlarge the growth of strains in culture, and the activity of the strains can also be remarkably improved. The improvement of the activity further enhances the growth promoting effect of the strain, thereby being beneficial to improving the quality of plants such as fruit nursery stocks and the like. In this way, the growth condition of the fruit tree nursery stock is remarkably improved, and then the overall yield is increased. Therefore, the invention has wide application prospect and remarkable economic value in the fields of agriculture and gardening.

Description

Technical Field

[0001] The present invention relates to the technical field of biological microbial agents, and in particular to a culture medium for growth-promoting bacteria endophytic in tree roots and a preparation method thereof. Background Art

[0002] The plant rhizosphere is a good habitat for bacteria. Plant roots provide a good ecological niche for bacteria. Bacteria that can colonize the plant rhizosphere continuously and stably and are affected by plants are called rhizobacteria. Plant rhizosphere bacteria have rich genetic diversity. The population density of rhizosphere bacteria is 100 times higher than that of non-rhizosphere soil. Up to 15% of the roots may be covered by microcolonies of various bacteria. Bacteria use nutrients released by plants (root secretions, lysates) to grow and reproduce, and also synthesize metabolites and secrete them into the rhizosphere. Some metabolites are sensed as signal transduction compounds by neighboring cells of the same microcolony, other bacterial cells or host plant cells (Van Loon, 2007). In 1978, JW Kloepper of Auburn University in the United States first proposed the concept of plant growth-promoting rhiz obacteria (PGPR). PGPR is a group of rhizobacteria that colonize the rhizosphere of plants and are closely related to plant roots. When inoculated into plant seeds, roots, tubers, rhizobium or soil, they can promote plant growth. PGPR is a self-living bacterium. Although some strains can invade plant tissues, they do not cause obvious symptoms of infection. PGPR does not include rhizobia and Frankia, which form a symbiotic structure with plants. The symbiotic nitrogen-adding effect of rhizobia and Frankia does not fall within the scope of the growth-promoting effect of PGPR. Associated nitrogen-fixing bacteria should fall within the scope of PGPR. Some rhizobia can also be considered as PGPR when they have a growth-promoting effect on non-leguminous plants. PGPR directly or indirectly promotes plant growth through one or more growth-promoting mechanisms, including direct stimulation of plants, increased nutrient absorption by plants, inhibition of plant diseases, and induction of plant systemic resistance. Direct growth-promoting effects include the production of stimulating plant hormones, volatile compounds (volatiles), and ACC deaminase, etc., reducing the ethylene level in the plant, improving the nutritional status of the plant (such as promoting the release of insoluble phosphorus, potassium and trace elements, non-symbiotic nitrogen fixation, etc.), and stimulating the plant to produce induced systemic resistance (induced systemic resistance, ISR). Indirect growth-promoting effects include the production of antibiotics, antimicrobial proteins or iron carriers to reduce diseases, promote beneficial symbiotic effects (the formation of nodules and mycorrhizae), and degrade biological exogenous substances such as pesticides. In most studies, a PGPR usually promotes plant growth through multiple modes of action.

[0003] In the prior art, growth-promoting rhizobacteria (PGPR) generally refers to rhizosphere microorganisms that can directly or indirectly promote plant growth, increase crop yields, and prevent and control pests and diseases. More than 20 species of PGPR have been discovered at home and abroad, including Pseudomonas, Bacillus, Agrobacterium, Flavobacterium, Serratia, etc. The screening of excellent PGPR strains generally involves selecting excellent PGPR strains from the rhizosphere microorganisms of plants, soil in non-pathogenic habitats, or marine microbial resources for mutagenesis breeding, or using genetic engineering methods such as gene cloning and gene deletion to obtain new high-efficiency and low-toxic production strains.

[0004] Chinese patent CN112266888A discloses a method for improving the biomass and spore production of root growth-promoting bacteria of Bacillus plants. The invention uses a wide range of raw materials such as crop straw and cassava residue as a carrier matrix of Bacillus plant root growth-promoting bacteria, which is low in cost and convenient for storing root growth-promoting bacteria of Bacillus plants, and maintains activity during transportation. At the same time, crop straw and cassava residue can also be fertilizers for plants, which can be degraded back into the soil to provide nutrition for the growth of plants, and are environmentally friendly and energy-saving. In addition, the carrier matrix formed by crop straw and cassava residue is mixed with limestone powder and isoleucine in advance. Limestone powder has a rich calcium carbonate component, and calcium carbonate and isoleucine promote Bacillus to produce spores, thereby improving the growth-promoting effect of crops.

[0005] However, the above method requires further mixing with a carrier matrix after the strain is expanded and cultured to prepare a bacterial agent. In the actual production process, this process is difficult to achieve. In the current technical system, there is a lack of a culture medium that can play a more efficient role. Summary of the invention

[0006] The purpose of the present invention is to provide a culture medium and a preparation method for endophytic growth-promoting bacteria in tree roots, thereby providing an effective means for the development of endophytic growth-promoting bacteria agents in tree roots.

[0007] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions: The present invention provides a tree root endophytic growth-promoting bacteria culture medium, comprising the following components: Trypticase soy broth, ammonium acetate, magnesium sulfate, pyridoxine, aspartic acid, aloin, and water.

[0008] Preferably, the culture medium comprises the following components in parts by weight: 20-40 parts of tryptic casein soy broth, 1-3 parts of ammonium acetate, 1-3 parts of magnesium sulfate, 0.5-1.5 parts of pyridoxine, 1-3 parts of aspartic acid, 1-3 parts of aloin and 800-1200 parts of water.

[0009] Preferably, the culture medium comprises the following components in parts by weight: Trypticase soy broth 30 parts, ammonium acetate 2 parts, magnesium sulfate 2 parts, pyridoxine 1 part, aspartic acid 2 parts, aloin 2 parts and water 1000 parts.

[0010] The present invention also provides a method for preparing the above-mentioned tree root endophytic growth-promoting bacteria culture medium, comprising the following steps: The tryptic casein soy broth, ammonium acetate, magnesium sulfate, pyridoxine, aspartic acid, aloin and water are mixed, the pH value is adjusted and then sterilized to obtain the tree root endophytic growth-promoting bacteria culture medium.

[0011] Preferably, the pH is 6.8-7.2.

[0012] Preferably, the sterilization temperature is 120-122°C.

[0013] Preferably, the sterilization time is 20 to 40 minutes.

[0014] The present invention also provides a method for preparing a growth-promoting bacterial agent, comprising the following steps: The endophytic growth-promoting bacteria in the roots of trees are inoculated into the above-mentioned endophytic growth-promoting bacteria culture medium for tree roots, and the growth-promoting bacteria agent is obtained by cultivation.

[0015] Preferably, the endophytic growth-promoting bacteria in the tree roots are boron-aggregating bacteria, and the culture temperature is 25-30°C.

[0016] The present invention also provides a growth-promoting bacterial agent prepared by the above preparation method.

[0017] Technical effects and advantages of the present invention: The culture medium involved in the present invention can not only provide the required nutrients to support and expand the growth of the strains in culture through the synergistic effect between its components, but also significantly improve the activity of these strains. The improvement of this activity further enhances the growth-promoting effect of the strains, thereby helping to improve the quality of plants such as fruit tree seedlings. In this way, the growth conditions of fruit tree seedlings are significantly improved, thereby increasing the overall yield. Therefore, the present invention has broad application prospects and significant economic value in the fields of agriculture and horticulture.

[0018] The culture medium preparation method involved in the present invention is characterized by simple operation and high cost-effectiveness, and is suitable for large-scale production. The provided culture medium provides a suitable growth environment for endophytic growth-promoting bacteria in tree roots, and significantly improves the survival rate and growth-promoting ability of the strain, thereby enhancing the efficiency and reliability of the product in practical applications. The present invention not only provides an innovative technical solution for the cultivation and application of endophytic growth-promoting bacteria in tree roots, but also has significant practical application value in promoting plant growth and increasing crop yield and quality. DETAILED DESCRIPTION

[0019] The present invention provides a tree root endophytic growth-promoting bacteria culture medium, comprising the following components: Tryptic casein soy broth, ammonium acetate, magnesium sulfate, pyridoxine, aspartic acid, aloin and water. Preferably, in parts by weight, the culture medium comprises the following components: 20-40 parts of tryptic casein soy broth, 1-3 parts of ammonium acetate, 1-3 parts of magnesium sulfate, 0.5-1.5 parts of pyridoxine, 1-3 parts of aspartic acid, 1-3 parts of aloin and 800-1200 parts of water. Preferably, in parts by weight, the culture medium comprises the following components: 30 parts of tryptic casein soy broth, 2 parts of ammonium acetate, 2 parts of magnesium sulfate, 1 part of pyridoxine, 2 parts of aspartic acid, 2 parts of aloin and 1000 parts of water.

[0020] The component formula of the culture medium for endophytic growth-promoting bacteria in the root system of trees involved in the present invention has the following significant advantages: First, the culture medium formula can provide extremely rich nutrients, which can fully meet the growth requirements of endophytic growth-promoting bacteria in the root system of trees, thereby ensuring that these beneficial microorganisms can thrive in a good environment. Secondly, by accurately controlling the proportion of each component in the culture medium, the reproduction speed and activity of the growth-promoting bacteria can be effectively promoted, thereby improving its colonization ability and work efficiency in the root system of plants. Third, the preparation method of the culture medium is simple and easy, the operation steps are simple, and it is suitable for large-scale production and application, which not only reduces the production cost, but also improves the production efficiency, making the culture medium highly feasible in practical applications. Fourth, the growth-promoting bacteria prepared using the culture medium can effectively improve the growth rate and quality of plants, make plants grow healthier and stronger, thereby improving the yield and quality of crops. Fifth, the use of the culture medium helps to reduce the use of fertilizers and pesticides, thereby reducing the burden on the environment, being environmentally friendly, and conforming to the concept of sustainable development. Finally, this culture medium and its preparation method provide a new solution for the healthy growth of plants such as fruit tree seedlings. It can not only improve the growth environment of plants, but also enhance the disease resistance of plants, thus playing an important role in agricultural production.

[0021] The present invention also provides a method for preparing the above-mentioned tree root endophytic growth-promoting bacteria culture medium, comprising the following steps: Tryptic casein soy broth, ammonium acetate, magnesium sulfate, pyridoxine, aspartic acid, aloin and water are mixed, and the pH is adjusted and then sterilized to obtain the tree root endophytic growth-promoting bacteria culture medium. Preferably, the pH is 6.8-7.2. Preferably, the sterilization temperature is 120-122° C. Preferably, the sterilization time is 20-40 min.

[0022] The present invention also provides a method for preparing a growth-promoting agent, comprising the following steps: inoculating the growth-promoting bacteria endophytic to the tree root system into the above-mentioned tree root system endophytic growth-promoting bacteria culture medium, and culturing to obtain the growth-promoting agent. Preferably, the tree root system endophytic growth-promoting bacteria is polyboron-grasping bacteria, and the culture temperature is 25-30°C. The present invention also provides a growth-promoting agent obtained by the above-mentioned preparation method.

[0023] In the present invention, the growth-promoting bacteria agent may also be added with other components, such as trace elements, vitamins and growth hormones required for plant growth, to further enhance the effect of the growth-promoting bacteria agent. In addition, the growth-promoting bacteria agent may also be mixed with other biological fertilizers or chemical fertilizers to achieve a better plant growth promotion effect. In practical applications, the growth-promoting bacteria agent can be used for the root systems of various plants such as fruit trees, vegetables, and flowers to promote their growth and increase yield. By using the growth-promoting bacteria agent of the present invention, the use of chemical fertilizers and pesticides can be reduced, thereby reducing agricultural production costs. At the same time, it is environmentally friendly and helps to achieve sustainable agricultural development.

[0024] In the present invention, the preparation method of the growth-promoting agent also includes the following steps: mixing the growth-promoting agent with water to prepare a liquid form suitable for absorption by plant roots. The growth-promoting agent in liquid form can be more conveniently applied to the rhizosphere of plants to promote the absorption and utilization of nutrients by plants. In addition, the application method of the growth-promoting agent also includes directly applying the growth-promoting agent to the soil, or sowing it with seeds to promote seed germination and seedling growth. During the application process, the application amount and frequency of the growth-promoting agent can be adjusted according to the type and growth stage of the plant to achieve the best growth-promoting effect. The growth-promoting agent of the present invention can effectively improve the disease resistance of plants, reduce the occurrence of diseases and insect pests, thereby reducing the use of chemical pesticides and further protecting the ecological environment.

[0025] In the present invention, the growth-promoting bacteria agent is preferably applied to fruit trees, and the application methods include the following: 1. Evenly spread in the rhizosphere soil of fruit trees; 2. Irrigate after mixing with water; 3. Apply after mixing with organic fertilizer; 4. Apply directly to the roots of fruit trees. The above application methods can be flexibly selected according to the type of fruit tree, growth stage and soil conditions to ensure that the growth-promoting bacteria agent can effectively play a role and promote the healthy growth of fruit trees. In addition, during the application process, the growth-promoting bacteria agent of the present invention can also improve the soil structure and increase the number of beneficial microorganisms in the soil, thereby further improving the growth environment of fruit trees.

[0026] In a specific embodiment of the present invention, the growth-promoting bacteria agent is diluted at a ratio of 1:500 and then applied to the roots. It is applied twice during the flowering period and the fruit expansion period of the apple tree, and the application amount each time is 5 liters / mu (calculated based on the original solution volume).

[0027] The microbial agent product involved in the present invention has a wide range of application value. It can not only be applied to a variety of different types of seedlings, but also can be combined with a variety of different carriers to prepare a more stable and efficient microbial agent product. This microbial agent product is of great significance in agricultural production. First, it can significantly improve the resistance of plants to various diseases, thereby effectively reducing the economic losses caused by diseases. Secondly, the microbial agent can promote the healthy development of plant roots, enhance the plant's ability to absorb water and nutrients, and thus improve the overall growth of plants. By improving the plant rhizosphere environment, the microbial agent helps to promote the proliferation of beneficial microorganisms, thereby forming a healthy soil microecological system. This healthy soil microecology can not only provide better growth conditions for plants, but also further enhance the resistance of plants to diseases. In terms of fruit tree planting, the microbial agent can effectively improve the quality of fruits, including improving the color, taste and nutritional value of fruits, making fruits more delicious and having higher nutritional value. The microbial agent product provided by the present invention is suitable for organic agriculture, which is fully in line with the green and environmentally friendly planting concept. It helps to produce pollution-free agricultural products and meet consumers' demand for healthy and safe food. Therefore, this microbial agent not only has a significant promoting effect on agricultural production, but also has important significance for environmental protection and sustainable development.

[0028] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0029] The biological materials used in the present invention are derived from: Variovorax boronicumulans was purchased from the National Strain Resource Bank with the strain number of CCTCC AB 2012269 and the platform resource number of 1542C0001WAB212269.

[0030] Example 1 Prepare the culture medium: 30 g of tryptic casein soy broth, 2 g of ammonium acetate, 2 g of magnesium sulfate, 1 g of pyridoxine, 2 g of aspartic acid, 2 g of aloin and 1 L of distilled water, adjust the pH of the system to 7.0 with 0.1 mol / L sodium hydroxide or 0.1 mol / L hydrochloric acid, heat to dissolve evenly, and maintain at 121°C for 30 min to obtain the tree root endophytic growth-promoting bacteria culture medium.

[0031] The activated polyborax phagocytes were inoculated into the above culture medium by aseptic operation, and cultured at 28°C, 150 rpm, and dark conditions for 72 hours to obtain a tree root growth-promoting bacterial agent.

[0032] Example 2 Prepare the culture medium: 30 g of tryptic casein soy broth, 1.5 g of ammonium acetate, 2.5 g of magnesium sulfate, 1 g of pyridoxine, 2 g of aspartic acid, 2 g of aloin and 1 L of distilled water, adjust the pH of the system to 7.0 with 0.1 mol / L sodium hydroxide or 0.1 mol / L hydrochloric acid, heat to dissolve evenly, and maintain at 121°C for 30 min to obtain the tree root endophytic growth-promoting bacteria culture medium.

[0033] The activated polyborax phagocytes were inoculated into the above culture medium by aseptic operation, and cultured at 28°C, 150 rpm, and dark conditions for 72 hours to obtain a tree root growth-promoting bacterial agent.

[0034] Example 3 Prepare the culture medium: 30 g of tryptic casein soy broth, 2.5 g of ammonium acetate, 1.5 g of magnesium sulfate, 1.5 g of pyridoxine, 2 g of aspartic acid, 2 g of aloin and 1 L of distilled water, adjust the pH of the system to 7.0 with 0.1 mol / L sodium hydroxide or 0.1 mol / L hydrochloric acid, heat to dissolve evenly, and maintain at 121°C for 30 min to obtain the tree root endophytic growth-promoting bacteria culture medium.

[0035] The activated polyborax phagocytes were inoculated into the above culture medium by aseptic operation, and cultured at 27°C, 150 rpm, and dark conditions for 72 hours to obtain a tree root growth-promoting bacterial agent.

[0036] Example 4 Prepare the culture medium: 30 g of tryptic casein soy broth, 2 g of ammonium acetate, 2 g of magnesium sulfate, 0.5 g of pyridoxine, 2.5 g of aspartic acid, 1.5 g of aloin and 1 L of distilled water, adjust the pH of the system to 7.0 with 0.1 mol / L sodium hydroxide or 0.1 mol / L hydrochloric acid, heat to dissolve evenly, and maintain at 121°C for 30 min to obtain the tree root endophytic growth-promoting bacteria culture medium.

[0037] The activated polyborax phagocytes were inoculated into the above culture medium by aseptic operation, and cultured at a constant temperature of 28°C, 150rpm, and dark culture conditions for 48 hours to obtain a tree root growth-promoting bacterial agent.

[0038] Example 5 Prepare the culture medium: 30 g of tryptic casein soy broth, 1.8 g of ammonium acetate, 2 g of magnesium sulfate, 1 g of pyridoxine, 1.5 g of aspartic acid, 2.5 g of aloin and 1 L of distilled water, adjust the pH of the system to 7.0 with 0.1 mol / L sodium hydroxide or 0.1 mol / L hydrochloric acid, heat to dissolve evenly, and maintain at 121°C for 30 min to obtain the tree root endophytic growth-promoting bacteria culture medium.

[0039] The activated polyborax phagocytes were inoculated into the above culture medium by aseptic operation, and cultured at 29°C, 150 rpm, and dark conditions for 72 hours to obtain a tree root growth-promoting bacterial agent.

[0040] Example 6 Prepare the culture medium: 30 g of tryptic casein soy broth, 2.2 g of ammonium acetate, 2.2 g of magnesium sulfate, 1 g of pyridoxine, 2 g of aspartic acid, 2 g of aloin and 1 L of distilled water, adjust the pH of the system to 7.0 with 0.1 mol / L sodium hydroxide or 0.1 mol / L hydrochloric acid, heat to dissolve evenly, and maintain at 121°C for 30 min to obtain the tree root endophytic growth-promoting bacteria culture medium.

[0041] The activated polyborax phagocytes were inoculated into the above culture medium by aseptic operation, and cultured at 28°C, 150 rpm, and dark conditions for 72 hours to obtain a tree root growth-promoting bacterial agent.

[0042] Example 7 Prepare the culture medium: 35 g of tryptic casein soy broth, 1.5 g of ammonium acetate, 2.5 g of magnesium sulfate, 1 g of pyridoxine, 1.8 g of aspartic acid, 2 g of aloin and 1 L of distilled water, adjust the pH of the system to 7.0 with 0.1 mol / L sodium hydroxide or 0.1 mol / L hydrochloric acid, heat to dissolve evenly, and maintain at 121°C for 30 min to obtain the tree root endophytic growth-promoting bacteria culture medium.

[0043] The activated polyborax phagocytes were inoculated into the above culture medium by aseptic operation, and cultured at 28°C, 150 rpm, and dark conditions for 72 hours to obtain a tree root growth-promoting bacterial agent.

[0044] Example 8 Prepare the culture medium: 25 g of tryptic casein soy broth, 2.5 g of ammonium acetate, 1.5 g of magnesium sulfate, 1.5 g of pyridoxine, 2 g of aspartic acid, 2.2 g of aloin and 1 L of distilled water, adjust the pH of the system to 7.0 with 0.1 mol / L sodium hydroxide or 0.1 mol / L hydrochloric acid, heat to dissolve evenly, and maintain at 121°C for 30 min to obtain the tree root endophytic growth-promoting bacteria culture medium.

[0045] The activated polyborax phagocytes were inoculated into the above culture medium by aseptic operation, and cultured at 27°C, 150 rpm, and dark conditions for 72 hours to obtain a tree root growth-promoting bacterial agent.

[0046] Example 9 Prepare the culture medium: 35 g of tryptic casein soy broth, 2 g of ammonium acetate, 2 g of magnesium sulfate, 0.5 g of pyridoxine, 2.5 g of aspartic acid, 1.8 g of aloin and 1 L of distilled water, adjust the pH of the system to 7.0 with 0.1 mol / L sodium hydroxide, heat to dissolve evenly, and maintain at 121°C for 30 min to obtain the tree root endophytic growth-promoting bacteria culture medium.

[0047] The activated polyborax was inoculated into the above culture medium by aseptic operation, and cultured at 28°C, 100 rpm, and dark conditions for 72 hours to obtain a tree root growth-promoting bacterial agent.

[0048] Example 10 Prepare the culture medium: 30 g of tryptic casein soy broth, 2 g of ammonium acetate, 2 g of magnesium sulfate, 1 g of pyridoxine, 1.5 g of aspartic acid, 2.5 g of aloin and 1 L of distilled water, adjust the pH of the system to 7.0 with 0.1 mol / L sodium hydroxide or 0.1 mol / L hydrochloric acid, heat to dissolve evenly, and maintain at 121°C for 30 min to obtain the tree root endophytic growth-promoting bacteria culture medium.

[0049] The activated polyborax was inoculated into the above culture medium by aseptic operation, and cultured at 29°C, 200 rpm, and dark conditions for 72 hours to obtain a tree root growth-promoting bacterial agent.

[0050] Comparative Example Prepare the culture medium: 30 g of tryptic casein soy broth, 3 g of sucrose and 1 L of distilled water, adjust the pH of the system to 7.0 with 0.1 mol / L sodium hydroxide or 0.1 mol / L hydrochloric acid, heat to dissolve evenly, and maintain at 121°C for 30 minutes to obtain the tree root endophytic growth-promoting bacteria culture medium.

[0051] The activated polyborax phagocytes were inoculated into the above culture medium by aseptic operation, and cultured at 28°C, 150 rpm, and dark conditions for 72 hours to obtain a tree root growth-promoting bacterial agent.

[0052] Experimental example An experiment was conducted to explore the efficacy of the invented microbial agent. The experimental site was set up in an orchard, and the research subjects were five-year-old apple seedlings. The experimental design included an experimental group (using the tree root growth-promoting microbial agent in Example 1) and two control groups, namely control group 1 (using the tree root growth-promoting microbial agent in the comparative example) and control group 2 (no tree root growth-promoting microbial agent was applied). Appropriate distances were maintained between the experimental plots of each group to avoid mutual influence.

[0053] During the experiment, the growth-promoting bacteria provided by the present invention was diluted at a ratio of 1:500 and then used for root irrigation. It was applied twice, during the flowering period and the fruit expansion period of the apple tree, with each application amount of 5 liters / mu (calculated based on the original liquid volume).

[0054] After harvest, the average yield of apples in each group was recorded. The results are shown in Table 1 below: Table 1 Yield results

[0055] The data processing results showed that the yield increase in the experimental group was significantly higher than that in the control group (P < 0.05).

[0056] The results show that the tree root growth-promoting bacteria agent provided by the present invention has a significant effect on promoting the growth of apple trees and increasing yield. The average single fruit weight, average single plant yield and average per mu yield of apple trees in the experimental group were higher than those in the control group, indicating that the growth-promoting bacteria agent can effectively promote plant growth and increase crop yield. In addition, compared with the control group 1, although the control group 1 applied the growth-promoting bacteria agent, its effect was significantly lower than that of the experimental group, which further proves the superiority of the growth-promoting bacteria agent obtained by culturing the specific culture medium provided by the present invention in promoting plant growth. The experimental results show that the growth-promoting bacteria agent of the present invention has good application prospects and economic value in practical applications.

[0057] In fact, the growth-promoting bacteria of the present invention not only has a significant promoting effect on the growth and yield of apple trees, but also shows good application effects in the planting of other fruit trees. For example, the growth-promoting bacteria of the present invention are applied to fruit trees such as peach trees, pear trees and citrus trees, and the promotion of plant growth and the increase in yield are also observed. In addition, the growth-promoting bacteria can also improve the resistance of fruit trees to diseases and pests, reduce the use of pesticides, and further promote the healthy growth of fruit trees and the improvement of fruit quality. In practical applications, the growth-promoting bacteria of the present invention can be combined with existing agricultural management measures to provide an efficient and environmentally friendly solution for fruit tree planting. Through the growth-promoting bacteria of the present invention, agricultural producers can achieve precise management of fruit tree growth, improve the overall economic benefits of orchards, and have a positive impact on environmental sustainability.

[0058] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A culture medium for endophytic growth-promoting bacteria in tree roots, characterized in that: Includes the following components: Trypticase soy broth, ammonium acetate, magnesium sulfate, pyridoxine, aspartic acid, aloin, and water.

2. The tree root endophytic growth-promoting bacteria culture medium according to claim 1, characterized in that: The culture medium comprises the following components in parts by weight: 20-40 parts of tryptic casein soy broth, 1-3 parts of ammonium acetate, 1-3 parts of magnesium sulfate, 0.5-1.5 parts of pyridoxine, 1-3 parts of aspartic acid, 1-3 parts of aloin and 800-1200 parts of water.

3. The tree root endophytic growth-promoting bacteria culture medium according to claim 2, characterized in that: The culture medium comprises the following components in parts by weight: Trypticase soy broth 30 parts, ammonium acetate 2 parts, magnesium sulfate 2 parts, pyridoxine 1 part, aspartic acid 2 parts, aloin 2 parts and water 1000 parts.

4. The method for preparing a culture medium for endophytic growth-promoting bacteria in tree roots according to any one of claims 1 to 3, characterized in that: The following steps are involved: The tryptic casein soy broth, ammonium acetate, magnesium sulfate, pyridoxine, aspartic acid, aloin and water are mixed, the pH value is adjusted and then sterilized to obtain the tree root endophytic growth-promoting bacteria culture medium.

5. The method for preparing a culture medium for endophytic growth-promoting bacteria in tree roots according to claim 4, characterized in that: The pH is 6.8~7.

2.

6. The method for preparing a culture medium for endophytic growth-promoting bacteria in tree roots according to claim 4, characterized in that: The sterilization temperature is 120-122°C.

7. The method for preparing a culture medium for endophytic growth-promoting bacteria in tree roots according to claim 4, characterized in that: The sterilization time is 20 to 40 minutes.

8. A method for preparing a growth-promoting bacterium agent, characterized in that: The following steps are involved: The endophytic growth-promoting bacteria in the roots of trees are inoculated into the endophytic growth-promoting bacteria culture medium for trees according to any one of claims 1 to 3, and the growth-promoting bacteria agent is obtained by cultivation.

9. The method for preparing the growth-promoting bacterium according to claim 8, characterized in that: The endophytic growth-promoting bacteria in the tree root system are polyboric phagocytic bacteria, and the culture temperature is 25-30°C.

10. A growth-promoting bacterial agent obtained by the preparation method according to claim 8 or 9.

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