Extraction device for nano antibody drug component detection

Abstract

The invention relates to the technical field of drug component detection, in particular to an extraction device for nano-antibody drug component detection, which comprises an extraction tank, a nano ultrafiltration membrane, a pressure increasing mechanism, a pressure increasing mechanism, a pressure increasing mechanism, a pressure increasing mechanism, a pressure increasing mechanism, a pressure increasing mechanism, a pressure increasing mechanism, a pressure increasing mechanism, a pressure increasing mechanism, a pressure increasing mechanism and a pressure increasing mechanism, and is characterized in that the pressure increasing mechanism is inserted into the upper end of the extraction tank; the stirring mechanism is arranged at the bottom of the extraction tank; the square pipe is communicated with the upper end opening of the extraction tank, and two polishing mechanisms are symmetrically and movably arranged on the two sides of the square pipe. The two polishing mechanisms are driven to move reversely through the opposite pressing mechanism, so that the two polishing mechanisms synchronously move towards the interior of a square tube, two polishing belts of the two polishing mechanisms are pressed on the two sides of a tablet correspondingly, and protective layers on the two sides of a nano antibody drug are polished and removed; the solution does not contain large-particle molecules in the protective layer, so that the resistance of nano-antibody drug component molecules passing through the nano ultrafiltration membrane is reduced, and the extraction efficiency of nano-antibody drug components is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of drug component detection, in particular to an extraction device for detecting nano antibody drug components. Background Art

[0002] Drug component testing is an important step in drug production. Through drug component extraction and testing, it is determined whether the active ingredients of the drug are qualified. Ordinary drugs usually use filter membranes to filter and separate drug solutions to extract the active ingredients in the drugs. For some nano-antibody drugs, the molecular structure of the active ingredients is smaller, and ordinary filter membranes cannot separate and extract the nano-antibody drug components well, so they need to be filtered through nano-ultrafiltration membranes.

[0003] After searching, a Chinese patent with publication number: CN117538133A discloses an extraction device for detecting antibody drug components, including: a base, on the upper side of which are installed multiple mixing tubes and a base, the multiple mixing tubes are distributed side by side and arranged in parallel with the base; sample tubes, the multiple sample tubes are placed inside the base and are respectively located on the axial extension lines of each mixing tube; a feeding box, the multiple feeding boxes are respectively connected to each mixing tube, and a solenoid valve is provided at the connecting position; a water supply pipe, which is connected to each mixing tube, and a solenoid valve is provided at the connecting position; an equipment box, which is connected to the base and is arranged on the upper side of each mixing tube and the base.

[0004] Based on the above search and combined with actual problems, it is found that the extraction device cannot be applied to nano-antibody drugs with smaller molecular structures. Since nano-antibody drugs need to pass through a nano-ultrafiltration membrane to separate and extract the nano-antibody drug component molecules, the filter pores are smaller, so the nano-antibody drug solution is subject to greater resistance, resulting in lower nano-antibody drug extraction efficiency. In addition, since nano-antibody drugs are generally in the form of sheets, a protective layer is usually provided on the outside of the tablet, such as a PEG polymer layer, a liposome layer, a protein shell layer, etc. The protective layer can help the nano-antibody drug to circulate and distribute better in the body, thereby improving the efficacy of the nano-antibody drug. However, when extracting the components in the nano-antibody drug, these protective layers will be dissolved together with the nano-antibody drug components, and the molecular structure of the protective layer material is generally larger than the molecular size of the nano-antibody drug components. Therefore, when extracting the nano-antibody drug components through a nano-ultrafiltration membrane, it will increase the difficulty of the nano-antibody drug passing through the filter pores of the nano-ultrafiltration membrane, further reducing the extraction efficiency of the nano-antibody drug. Summary of the invention

[0005] The object of the present invention is to provide an extraction device for detecting nano-antibody drug components to solve the problems raised in the above-mentioned background technology.

[0006] The technical solution of the present invention is: an extraction device for detecting nano-antibody drug components, comprising an extraction tank, a solvent filling pipe is inserted at the upper end of the extraction tank, and also comprises: a nano-ultrafiltration membrane, a pressurizing mechanism is arranged between the nano-ultrafiltration membrane and the extraction tank; a stirring mechanism is arranged at the bottom of the extraction tank; a square tube connected to the upper port of the extraction tank, two grinding mechanisms are symmetrically and movably arranged on both sides of the square tube, and a pressure mechanism is arranged between the two grinding mechanisms and the square tube; the grinding mechanism comprises two rectangular plates movably arranged on one side of the square tube, belt rollers are rotatably connected at the four corners between the two rectangular plates, and a grinding motor is installed on one side of one of the rectangular plates, the driving end of the grinding motor is connected to one end of one of the belt rollers, and the outer sides of the four belt rollers are rotatably sleeved with grinding belts, and a plurality of scraping strips are evenly spaced on the outer side of the grinding belt.

[0007] Preferably, the counter-pressure mechanism includes a straight rail fixed on one side of the square tube, a counter-pressure driving motor is installed at one end of the straight rail, a threaded shaft located on the inner side of the straight rail is fixed to the driving end of the counter-pressure driving motor, and two threaded sliders are rotatably connected to the outer ends of the threaded shaft through two threads in opposite directions, and two counter-pressure connecting rods are symmetrically rotatably connected on both sides of the two threaded sliders, and one end of the two counter-pressure connecting rods located on the same side are rotatably connected to one side of the rectangular plate at the corresponding position.

[0008] Preferably, a blocking plate is slidably inserted at the middle position of the lower end of the square tube to isolate the square tube and the extraction tank, and a first electric cylinder is installed at the position of the blocking plate on the outside of the square tube, and one end of the blocking plate is fixed to the telescopic end of the first electric cylinder through an extension plate.

[0009] Preferably, a receiving groove is provided inside the baffle plate, and a grid plate is provided on the upper side of the baffle plate.

[0010] Preferably, cleaning drive motors are installed on both sides of the square tube at positions above the two grinding belts, cleaning disks are fixed to the driving ends of the two cleaning drive motors, and bristles are evenly arranged on the edges of the two cleaning disks.

[0011] Preferably, an inner support plate is fixed between the two rectangular plates on the same side at one side position, and one side of the inner support plate is in contact with the inner side of the grinding belt.

[0012] Preferably, two guiding inclined surfaces are symmetrically arranged on the inner upper end of the square tube.

[0013] Preferably, the boosting mechanism includes a liquid inlet pipe inserted at the bottom end of the extraction tank and a liquid outlet pipe inserted at the liquid inlet end of the nano ultrafiltration membrane, a pressure cylinder is connected between the liquid inlet pipe and the liquid outlet pipe, a second electric cylinder is installed at one end of the pressure cylinder, a first piston plate located on the inner side of the pressure cylinder is fixed to the telescopic end of the second electric cylinder, a plurality of piston guide rods are slidably inserted inside the first piston plate, a second piston plate is fixed at one end of each of the piston guide rods, and a pressure-maintaining spring is sleeved on the outer side of each piston guide rod, the first piston plate and the second piston plate are both slidably connected to the inner side of the pressure cylinder, and a one-way valve is provided on the inner side of the liquid inlet pipe and the liquid outlet pipe.

[0014] Preferably, the stirring mechanism includes a stirring motor installed at the lower end of the outer side of the extraction tank, the driving end of the stirring motor is fixed with a driving shaft extending to the inside of the extraction tank, the upper end of the driving shaft is fixed with a plurality of stirring rods arranged in a circle, and a fixed ring is fixed at the middle position of the outer side of the driving shaft, the lower end of the outer side of the driving shaft is connected to a movable ring through a spline, two crushing rollers are rotatably connected to both sides of the movable ring, and the upper end of the movable ring is elastically connected to the fixed ring through a pressure spring.

[0015] Preferably, multiple crushing convex points are evenly fixed on the outer sides of the two crushing rollers.

[0016] The present invention provides an extraction device for detecting nano antibody drug components through improvement, which has the following improvements and advantages compared with the prior art:

[0017] First, the present invention drives the two grinding mechanisms to move in the opposite direction through the pressing mechanism, so that the two grinding mechanisms move synchronously toward the inside of the square tube, and the two grinding belts of the two grinding mechanisms are respectively pressed on the two sides of the tablet. When the two grinding belts of the two grinding mechanisms rotate in the opposite direction, the protective layers on the two sides of the tablet can be polished and removed to ensure the purity of the nano-antibody drug. When the nano-antibody drug is dissolved in the solvent, the solution does not contain molecules of larger particles in the protective layer, so that the resistance encountered by the nano-antibody drug component molecules when passing through the filter pores of the nano-ultrafiltration membrane is smaller, thereby improving the extraction efficiency of the nano-antibody drug components.

[0018] Second, the present invention stirs the tablets and solvent put into the extraction tank through a stirring mechanism, and simultaneously rolls and crushes the tablets, thereby increasing the contact area between the nano-antibody drug and the solvent, increasing the speed at which the nano-antibody drug dissolves in the solvent, and ensuring the uniformity of the distribution of the nano-antibody drug components in the solution, thereby further improving the extraction efficiency of the nano-antibody drug components.

[0019] Thirdly, the present invention uses a pressurizing mechanism to press the nano-antibody drug solution into the interior of the nano-ultrafiltration membrane at a certain pressure, and continuously applies pressure to the nano-antibody drug solution to increase the speed at which the nano-antibody drug component molecules pass through the filter pores of the nano-ultrafiltration membrane, thereby further improving the extraction efficiency of the nano-antibody drug components. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1 It is a schematic diagram of the structure of the present invention from a first viewing angle;

[0022] Figure 2 It is a partial enlarged structural schematic diagram of the present invention;

[0023] Figure 3 It is a first cross-sectional structural schematic diagram of the present invention;

[0024] Figure 4 For the present invention Figure 3 A schematic diagram of the enlarged structure at A in the middle;

[0025] Figure 5 It is a second cross-sectional structural schematic diagram of the present invention;

[0026] Figure 6 For the present invention Figure 5 A schematic diagram of the enlarged structure at B in the middle;

[0027] Figure 7 For the present invention Figure 5 Schematic diagram of the enlarged structure at point C in the middle.

[0028] Reference numerals:

[0029] 1. Extraction tank; 2. Nano ultrafiltration membrane; 3. Square tube; 4. Solvent filling pipe; 5. Cleaning drive motor; 6. Cleaning plate; 101. Rectangular plate; 102. Grinding motor; 103. Belt roller; 104. Grinding belt; 105. Scraper; 106. Straight rail; 107. Counter-pressure drive motor; 108. Threaded shaft; 109. Threaded slider; 110. Counter-pressure connecting rod; 111. Blocking plate; 112. Grid plate; 113. First electric cylinder; 114. Extension Plate; 115, inner support plate; 116, guide slope; 201, pressure cylinder; 202, liquid inlet pipe; 203, liquid outlet pipe; 204, second electric cylinder; 205, first piston plate; 206, piston guide rod; 207, second piston plate; 208, pressure-maintaining spring; 301, stirring motor; 302, driving shaft; 303, stirring rod; 304, fixed ring; 305, movable ring; 306, pressure spring; 307, crushing roller; 308, crushing convex point. DETAILED DESCRIPTION

[0030] The present invention is described in detail below, and the technical solutions in the embodiments of the present invention are clearly and completely described. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] The present invention provides an extraction device for detecting a nano antibody drug component through improvement. The technical solution of the present invention is:

[0032] like Figures 1 to 7 As shown, the embodiment of the present invention provides an extraction device for detecting a nano antibody drug component, comprising an extraction tank 1, a solvent filling pipe 4 is inserted at the upper end of the extraction tank 1, and further comprising: a nano ultrafiltration membrane 2, a pressurizing mechanism is arranged between the nano ultrafiltration membrane 2 and the extraction tank 1; a stirring mechanism is arranged at the bottom of the extraction tank 1; a square tube 3 connected to the upper port of the extraction tank 1, two grinding mechanisms are symmetrically arranged on both sides of the square tube 3, and a counter-pressure mechanism is arranged between the two grinding mechanisms and the square tube 3; the grinding mechanism includes two rectangular plates 101 (such as Figure 2 and Figure 6 As shown, belt rollers 103 are rotatably connected at the four corners between the two rectangular plates 101, and a grinding motor 102 is installed on one side of one of the rectangular plates 101, and the driving end of the grinding motor 102 is connected to one end of one of the belt rollers 103, and the outer rotating sleeves of the four belt rollers 103 are provided with grinding belts 104, and multiple scraping strips 105 are evenly spaced on the outside of the grinding belt 104.

[0033] Furthermore, the counter-pressure mechanism includes a straight rail 106 fixed to one side of the square tube 3, a counter-pressure driving motor 107 is installed at one end of the straight rail 106, a threaded shaft 108 located on the inner side of the straight rail 106 is fixed to the driving end of the counter-pressure driving motor 107, two threaded sliders 109 are rotatably connected to the outer ends of the threaded shaft 108 through two threads in opposite directions, and two counter-pressure connecting rods 110 are symmetrically rotatably connected to the two sides of the two threaded sliders 109, and one end of the two counter-pressure connecting rods 110 located on the same side is rotatably connected to one side of the rectangular plate 101 at the corresponding position;

[0034] The pressing mechanism is used to drive the two grinding mechanisms to move synchronously toward the inside of the square tube 3, thereby applying pressure to both sides of the tablet, thereby facilitating grinding and removing the protective layer on the outer side of the tablet.

[0035] Furthermore, a blocking plate 111 is slidably inserted at the middle position of the lower end of the square tube 3 to isolate the square tube 3 and the extraction tank 1, and a first electric cylinder 113 is installed at the position of the blocking plate 111 on the outer side of the square tube 3, and one end of the blocking plate 111 is fixed to the telescopic end of the first electric cylinder 113 through an extension plate 114;

[0036] The telescopic end of the first electric cylinder 113 is controlled to extend to drive the blocking plate 111 to extend to the outside of the square tube 3, and then the tablets with the protective layer polished off fall into the interior of the extraction tank 1. The solvent for dissolving the nano antibody drug is added into the extraction tank 1 through the solvent filling pipe 4 to dissolve the tablets.

[0037] Furthermore, a receiving groove is provided inside the baffle plate 111, and a grid plate 112 is provided on the upper side of the baffle plate 111;

[0038] The scraped-off protective layer debris falls into the receiving groove inside the blocking plate 111 , preventing the protective layer debris from falling into the extraction tank 1 along with the tablets.

[0039] Furthermore, cleaning drive motors 5 are installed on both sides of the square tube 3 above the two grinding belts 104, and cleaning discs 6 are fixed to the driving ends of the two cleaning drive motors 5, and bristles are evenly arranged on the edges of the two cleaning discs 6;

[0040] The cleaning drive motor 5 drives the cleaning disc 6 to rotate, and the cleaning disc 6 drives multiple bristles on its outer side to rotate at high speed, which can clean the protective layer debris adhering to the outer side of the grinding belt 104, keep the outer side of the grinding belt 104 clean, and prevent the protective layer debris from sticking to the surface of the tablet again.

[0041] Furthermore, an inner support plate 115 is fixed between the two rectangular plates 101 on the same side at one side, and one side of the inner support plate 115 is in contact with the inner side of the grinding belt 104;

[0042] The inner support plate 115 supports the inner side of the grinding belt 104 so that the supported section of the grinding belt 104 maintains a straight line shape, thereby applying equal pressure to the multiple tablets stacked inside the square tube 3, thereby maintaining the uniformity of grinding the multiple tablets.

[0043] Furthermore, two guiding inclined surfaces 116 are symmetrically arranged on the inner upper end of the square tube 3;

[0044] Under the guidance of the two guiding inclined surfaces 116, the tablets fall vertically on the inner side of the square tube 3, so that the two sides of the tablets correspond to the two grinding belts 104 respectively, thereby improving the grinding uniformity.

[0045] Furthermore, the pressurizing mechanism includes a liquid inlet pipe 202 inserted at the bottom end of the extraction tank 1 and a liquid outlet pipe 203 inserted at the liquid inlet end of the nano ultrafiltration membrane 2, a pressure cylinder 201 is connected between the liquid inlet pipe 202 and the liquid outlet pipe 203, a second electric cylinder 204 is installed at one end of the pressure cylinder 201, a first piston plate 205 located on the inner side of the pressure cylinder 201 is fixed to the telescopic end of the second electric cylinder 204, a plurality of piston guide rods 206 are slidably inserted inside the first piston plate 205, a second piston plate 207 is fixed at one end of each piston guide rod 206, and a pressure retaining spring 208 is sleeved on the outer side of each piston guide rod 206, the first piston plate 205 and the second piston plate 207 are both slidably connected to the inner side of the pressure cylinder 201, and a one-way valve is provided on the inner side of the liquid inlet pipe 202 and the liquid outlet pipe 203;

[0046] The nano-antibody drug solution is pressed into the nano-ultrafiltration membrane 2 at a certain pressure through the booster mechanism, and pressure is continuously applied to the nano-antibody drug solution to increase the speed at which the nano-antibody drug component molecules pass through the filter pores of the nano-ultrafiltration membrane 2, thereby further improving the extraction efficiency of the nano-antibody drug components.

[0047] Furthermore, the stirring mechanism includes a stirring motor 301 installed at the lower end of the outer side of the extraction tank 1, a driving shaft 302 extending to the inside of the extraction tank 1 is fixed to the driving end of the stirring motor 301, a plurality of stirring rods 303 arranged in a circumference are fixed to the upper end of the driving shaft 302, and a fixed ring 304 is fixed to the outer side of the driving shaft 302 at the middle position, a movable ring 305 is connected to the lower end of the outer side of the driving shaft 302 through a spline, two crushing rollers 307 are rotatably connected to the two sides of the movable ring 305, and the upper end of the movable ring 305 is elastically connected to the fixed ring 304 through a pressure spring 306;

[0048] The tablets and solvent put into the extraction tank 1 are stirred by the stirring mechanism, and the tablets are rolled and crushed at the same time, thereby increasing the contact area between the nano-antibody drug and the solvent, increasing the speed at which the nano-antibody drug dissolves in the solvent, and ensuring the uniformity of the distribution of the nano-antibody drug components in the solution, thereby further improving the extraction efficiency of the nano-antibody drug components.

[0049] Furthermore, multiple crushing convex points 308 are evenly fixed on the outer sides of the two crushing rollers 307;

[0050] The crushing protrusions 308 improve the crushing effect on the tablet, making the tablet dissolve faster.

[0051] Working principle: When in use, the tablets to be extracted are dropped from the upper end of the square tube 3. Under the guidance of the two guiding slopes 116, the tablets fall vertically on the inner side of the square tube 3. Since some nano-antibody drugs are provided with a protective layer on the outside, including a PEG polymer layer, a liposome layer, a protein shell layer, etc., the protective layer can help the nano-antibody drugs to circulate and distribute better in the body, thereby improving the efficacy of the nano-antibody drugs. However, when extracting the components in the nano-antibody drugs, these protective layers will be dissolved together with the nano-antibody drug components, and the molecular structure of the protective layer material is generally larger than the molecular size of the nano-antibody drug components. Therefore, when the nano-antibody drug components are extracted through the nano-ultrafiltration membrane 2, it will increase the difficulty of the nano-antibody drug passing through the filter pores of the nano-ultrafiltration membrane 2, thereby reducing the extraction efficiency of the nano-antibody drug.

[0052] Therefore, it is necessary to process the nano antibody drug before dissolution. After the nano antibody drug falls into the inner side of the square tube 3, the pressure driving motor 107 of the pressure mechanism is controlled to drive the threaded shaft 108 to rotate. Since the outer ends of the threaded shaft 108 are connected to two threaded sliders 109 by two threads rotating in opposite directions, the threaded shaft 108 drives the two threaded sliders 109 to move in opposite directions through the threads when the threaded shaft 108 rotates. The two threaded sliders 109 respectively drive the four pressure connecting rods 110 to rotate in opposite directions, thereby driving the two grinding mechanisms on both sides to move synchronously toward the inner side of the square tube 3, so that the outer sides of the two grinding belts 104 of the two grinding mechanisms are respectively attached to the two sides of the tablet, and then the two grinding motors 102 of the two grinding mechanisms are controlled. In reverse operation, the grinding motor 102 drives the belt rollers 103 at the corresponding positions to rotate, thereby driving the two grinding belts 104 to rotate in the opposite direction. The two grinding belts 104 apply downward friction to the tablets, so that the tablets are closely attached to the upper side of the grid plate 112. When the two grinding belts 104 rotate, they drive the multiple scraping strips 105 on the outside to scrape the two sides of the tablets, so that the protective layers on both sides of the tablets can be scraped off, thereby ensuring the purity of the nano antibody drug. When the nano antibody drug is dissolved in the solvent, the solution does not contain the molecules of the larger particles in the protective layer, so that the nano antibody drug component molecules are subjected to less resistance when passing through the filter holes of the nano ultrafiltration membrane 2, thereby improving the extraction efficiency of the nano antibody drug components;

[0053] The scraped protective layer debris falls into the receiving groove inside the baffle plate 111, and then the telescopic end of the first electric cylinder 113 is controlled to extend to drive the baffle plate 111 and the grid plate 112 to extend to the outside of the square tube 3, and then the tablets with the protective layer polished off fall into the inside of the extraction tank 1, and the solvent for dissolving the nano antibody drug is added into the extraction tank 1 through the solvent filling pipe 4;

[0054] At the same time, the stirring motor 301 of the stirring mechanism drives the driving shaft 302 to rotate, and the driving shaft 302 drives the multiple stirring rods 303 on its outer side to rotate to stir the nano antibody drug and the solvent in the extraction tank 1, thereby improving the dissolution efficiency of the nano antibody drug and the solvent. At the same time, when the driving shaft 302 rotates, it can drive the movable ring 305 to rotate synchronously, and the movable ring 305 drives the two crushing rollers 307 on both sides thereof to rotate synchronously around the axis of the driving shaft 302. Because under the pressure of the pressure spring 306, the pressure spring 306 applies downward pressure to the movable ring 305, and the movable ring 305 applies downward pressure to the two crushing rollers 307, so that the two crushing rollers 307 apply a certain pressure to the nano antibody drug at the bottom of the extraction tank 1, so that when the two crushing rollers 307 rotate, the nano antibody drug can be rolled and crushed, thereby improving the dissolution efficiency of the nano antibody drug, thereby helping to improve the extraction efficiency of the nano antibody drug component;

[0055] After the nano antibody drug is completely dissolved in the solvent, the telescopic end of the second electric cylinder 204 of the pressurizing mechanism is retracted, and the telescopic end of the second electric cylinder 204 drives the first piston plate 205 and the second piston plate 207 to move toward one end of the pressure cylinder 201. Since both the inner sides of the liquid inlet pipe 202 and the liquid outlet pipe 203 are provided with a one-way valve, the conduction direction of the two one-way valves is upward. Therefore, when the first piston plate 205 and the second piston plate 207 move toward one end, the space at the other end of the pressure cylinder 201 is enlarged, so that the nano antibody drug solution inside the extraction tank 1 is sucked in and then the second electric cylinder 204 is controlled. The telescopic end of the pressure cylinder 201 extends outward, pushing the first piston plate 205 and the second piston plate 207 to move toward the other end of the pressure cylinder 201, pressing the nano antibody drug solution at the other end of the pressure cylinder 201 into the inside of the liquid outlet pipe 203, and then flowing into the inner side of the nano ultrafiltration membrane 2 through the liquid outlet pipe 203. The pore size of the nano ultrafiltration membrane 2 can be selected according to the size of the component molecules in the nano antibody drug, so that the nano antibody drug components can pass through the filter pores of the nano ultrafiltration membrane 2, while molecules with larger sizes cannot pass through, thereby effectively extracting the nano antibody drug components, thereby improving the extraction efficiency and extraction purity;

[0056] Since the second piston plate 207 and the first piston plate 205 are elastically connected by multiple pressure-maintaining springs 208, when the second piston plate 207 squeezes the nano-antibody drug solution inside the pressure cylinder 201, it will be subjected to the reaction force of the nano-antibody drug solution, thereby compressing the multiple pressure-maintaining springs 208. When the telescopic end of the second electric cylinder 204 stops moving, the multiple compressed pressure-maintaining springs 208 can still continue to apply thrust to the second piston plate 207. The second piston plate 207 applies a certain pressure to the nano-antibody drug solution, thereby keeping the nano-antibody drug solution inside the nano-ultrafiltration membrane 2 continuously under pressure, increasing the speed at which the nano-antibody drug molecules pass through the filter pores, and further improving the extraction efficiency of the nano-antibody drug components.

[0057] The above description enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. An extraction device for detecting a nano-antibody drug component, comprising an extraction tank (1), wherein a solvent filling pipe (4) is inserted at the upper end of the extraction tank (1), characterized in that: Also includes: A nano-ultrafiltration membrane (2), wherein a pressurizing mechanism is provided between the nano-ultrafiltration membrane (2) and the extraction tank (1); A stirring mechanism arranged at the bottom of the extraction tank (1); A square tube (3) connected to the upper port of the extraction tank (1), two grinding mechanisms being symmetrically and movably arranged on both sides of the square tube (3), and a pressing mechanism being arranged between the two grinding mechanisms and the square tube (3); The grinding mechanism comprises two rectangular plates (101) movably arranged on one side of the square tube (3); belt rollers (103) are rotatably connected between the two rectangular plates (101) at four corners; a grinding motor (102) is installed on one side of one of the rectangular plates (101); a driving end of the grinding motor (102) is connected to one end of one of the belt rollers (103); grinding belts (104) are rotatably sleeved on the outer sides of the four belt rollers (103); and a plurality of scraping strips (105) are equidistantly arranged on the outer sides of the grinding belts (104).

2. The extraction device for detecting a nano-antibody drug component according to claim 1, characterized in that: The counter-pressure mechanism comprises a straight rail (106) fixed on one side of the square tube (3); a counter-pressure driving motor (107) is installed on one end of the straight rail (106); a threaded shaft (108) located on the inner side of the straight rail (106) is fixed to the driving end of the counter-pressure driving motor (107); two threaded sliders (109) are rotatably connected to the outer ends of the threaded shaft (108) through two threads in opposite directions; two counter-pressure connecting rods (110) are symmetrically rotatably connected to the two sides of the two threaded sliders (109); one end of the two counter-pressure connecting rods (110) located on the same side are both rotatably connected to one side of the rectangular plate (101) at the corresponding position.

3. The extraction device for detecting a nano antibody drug component according to claim 1, characterized in that: A blocking plate (111) is slidably inserted at the middle position of the lower end of the square tube (3) to isolate the square tube (3) and the extraction tank (1), and a first electric cylinder (113) is installed at a position on the outer side of the square tube (3) corresponding to the blocking plate (111), and one end of the blocking plate (111) is fixed to the telescopic end of the first electric cylinder (113) via an extension plate (114).

4. The extraction device for detecting a nano-antibody drug component according to claim 3, characterized in that: The baffle plate (111) is provided with a receiving groove inside, and a grid plate (112) is arranged on the upper side of the baffle plate (111).

5. The extraction device for detecting a nano-antibody drug component according to claim 2, characterized in that: Cleaning drive motors (5) are installed on both sides of the square tube (3) above the two grinding belts (104), and cleaning discs (6) are fixed to the driving ends of the two cleaning drive motors (5), and bristles are evenly arranged on the edges of the two cleaning discs (6).

6. The extraction device for detecting a nano antibody drug component according to claim 1, characterized in that: An inner support plate (115) is fixed between the two rectangular plates (101) located on the same side at one side edge, and one side of the inner support plate (115) is in contact with the inner side of the grinding belt (104).

7. The extraction device for detecting a nano-antibody drug component according to claim 1, characterized in that: Two guiding inclined surfaces (116) are symmetrically arranged on the inner upper end of the square tube (3).

8. The extraction device for detecting a nano-antibody drug component according to claim 1, characterized in that: The pressurizing mechanism comprises a liquid inlet pipe (202) inserted at the bottom end of the extraction tank (1) and a liquid outlet pipe (203) inserted at the liquid inlet end of the nano-ultrafiltration membrane (2); a pressure cylinder (201) is connected between the liquid inlet pipe (202) and the liquid outlet pipe (203); a second electric cylinder (204) is installed at one end of the pressure cylinder (201); a first piston plate (205) located on the inner side of the pressure cylinder (201) is fixed to the telescopic end of the second electric cylinder (204); A plurality of piston guide rods (206) are slidably inserted inside the plug plate (205), a second piston plate (207) is fixed to one end of each of the piston guide rods (206), and a pressure retaining spring (208) is sleeved on the outer side of each piston guide rod (206), the first piston plate (205) and the second piston plate (207) are both slidably connected to the inner side of the pressure cylinder (201), and a one-way valve is provided on the inner side of the liquid inlet pipe (202) and the liquid outlet pipe (203).

9. The extraction device for detecting a nano antibody drug component according to claim 1, characterized in that: The stirring mechanism comprises a stirring motor (301) mounted at the lower end of the outer side of the extraction tank (1); a driving shaft (302) extending into the interior of the extraction tank (1) is fixed to the driving end of the stirring motor (301); a plurality of stirring rods (303) arranged in a circumferential pattern are fixed to the upper end of the driving shaft (302); a fixed ring (304) is fixed to the outer side of the driving shaft (302) at a middle position; a movable ring (305) is connected to the lower end of the outer side of the driving shaft (302) via a spline; two crushing rollers (307) are rotatably connected to the two sides of the movable ring (305); and the upper end of the movable ring (305) is elastically connected to the fixed ring (304) via a pressure spring (306).

10. A nano-antibody drug component detection extraction device according to claim 9, characterized in that: Multiple crushing convex points (308) are evenly fixed on the outer sides of the two crushing rollers (307).

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