Plant growth regulator containing 28-epiheight brassinolide and benzylaminopurine

Abstract

The invention discloses a plant growth regulator containing 28-epiheight brassinolide and benzylaminopurine, the mass ratio of the 28-epiheight brassinolide to the benzylaminopurine is (1 to 5): (124.75 to 1996), and the preferable ratio is (1 to 3): (249.5 to 1996). After the plant growth regulator containing the 28-epiheight brassinolide and the benzylaminopurine is applied to the leaf surfaces of the oilseed rape, the yield of the oilseed rape is obviously increased, and the quality of the oilseed rape is obviously improved.

Description

Technical Field

[0001] The invention belongs to the technical field of growth regulators, and particularly relates to a plant growth regulator comprising 28-epihomobrassinolide and benzylaminopurine. Background Art

[0002] In recent years, high quality, high yield and high efficiency have become the inevitable trend of the development of rapeseed industry. Rapeseed is one of the widely planted oil crops and occupies an important position in the field of oilseed planting. Rapeseed oil is one of the main edible vegetable oils, accounting for about 40% of the total edible vegetable oil. Rapeseed is one of the important sources of vegetable oil and feed protein. The average annual production of rapeseed oil is about 5.2 million tons and the annual feed meal is about 8 million tons, which plays an important role in ensuring the safety of edible oil. The evaluation of rapeseed quality involves four aspects, including seed quality, rapeseed quality, rapeseed oil quality and meal quality. Among them, rapeseed quality is one of the important aspects of rapeseed quality evaluation. Rapeseed quality evaluation indicators include oil content, erucic acid, glucosinolates and protein content. Among them, oleic acid, as a monounsaturated fatty acid, has become one of the key factors in measuring rapeseed quality due to its high nutritional value and health benefits; erucic acid is a specific long-chain fatty acid accumulated in rapeseed grains, which has adverse effects on human health; after being ingested by animals, glucosinolates may cause goiter, metabolic disorders, and growth and development stunting, etc. Reducing the content of erucic acid and glucosinolates is of great significance for improving the quality of rapeseed. Therefore, the target quality of rapeseed cultivation is to achieve higher oil content, oil yield and high-quality rapeseed oil. In practical production, high meal protein yield is also an important quality indicator. Therefore, how to efficiently increase rapeseed yield and quality and improve rapeseed agronomic traits has become the focus of current oil development.

[0003] Brassinolide is a new type of green and environmentally friendly plant growth regulator. It can promote the growth of vegetables, melons, fruits and other crops by soaking seeds with appropriate concentrations of brassinolide and spraying them on stems and leaves, making them brighter in color and thicker in leaves. It can also advance the time of tea leaf picking and make melons and fruits have higher sugar content, larger individuals, higher yields and better storage resistance. Benzylaminopurine is an organic compound and the first generation of synthetic cytokinins. It stimulates cell division to cause plant growth and development, inhibits respiratory kinases, and thus prolongs the freshness of green vegetables. It is also a widely used cytokinin added to plant growth medium.

[0004] However, there is no report on the application of 28-epihomobrassinolide and benzylaminopurine compounded plant growth regulators in increasing rapeseed yield, improving quality, and improving agronomic traits. Therefore, the present invention compounds 28-epihomobrassinolide with benzylaminopurine to study its effect on rapeseed growth regulation. Summary of the invention

[0005] The purpose of the present invention is to overcome the defects of the prior art and provide a plant growth regulator comprising 28-epihomobassinolide and benzylaminopurine, which can be used for rapeseed growth regulation to improve the quality and yield of rapeseed.

[0006] To achieve the above object, the present invention adopts the following technical solution:

[0007] A plant growth regulator comprising 28-epihomobrassinolide and benzylaminopurine, wherein the active ingredient of the plant growth regulator is a mixture of 28-epihomobrassinolide and benzylaminopurine.

[0008] Specifically, in the plant growth regulator comprising 28-epihomobassinolactone and benzylaminopurine, the mass ratio of 28-epihomobassinolactone to benzylaminopurine may be 1-5:124.75-1996. Among them, the mass ratio of 28-epihomobassinolactone to benzylaminopurine is further preferably 1-3:249.5-1996. The mass ratio of 28-epihomobassinolactone to benzylaminopurine is most preferably 1:499.

[0009] The plant growth regulator containing 28-epihomobassinolide and benzylaminopurine of the present invention can be made into a dosage form allowed by pesticides by using active ingredients and adjuvants. The dosage form includes but is not limited to wettable powder, soluble powder, water-dispersible granules, suspension, emulsifiable concentrate, emulsion in water, microemulsion or microcapsule, etc. The preferred dosage form is a soluble liquid.

[0010] The present invention also provides the use of the plant growth regulator containing 28-epihomobassinolide and benzylaminopurine in regulating the growth of rapeseed. The plant growth regulator can be applied during the growth period of rapeseed.

[0011] When the plant growth regulator comprising 28-epi-homobrassinolide and benzylaminopurine of the present invention is used to regulate the growth of rapeseed, preferably, the plant growth regulator can be applied to the rapeseed seedling stage and / or after transplanting and returning to green. Specifically, the total mass concentration of 28-epi-homobrassinolide and benzylaminopurine can be measured, and the plant growth regulator is diluted with water to prepare a liquid with an active ingredient concentration of 1.2574-20.01 mg / L (preferably a liquid with a total active ingredient concentration of 2.505-19.99 mg / L, most preferably a liquid of 5 mg / L), and sprayed on the aboveground leaf surface of rapeseed at the rapeseed seedling stage and / or after transplanting and returning to green. When the plant growth regulator of the present invention is used to regulate the growth of rapeseed, the plant growth regulator can effectively increase the plant height, stem thickness, number of effective branches, number of effective siliques, number of effective grains, and thousand-grain weight of rapeseed, improve the agronomic traits of rapeseed, and provide a basis for increasing yield.

[0012] The present invention also provides the use of the plant growth regulator containing 28-epihomobassinolide and benzylaminopurine in increasing the stress resistance of rapeseed. The plant growth regulator can effectively increase the stem thickness of rapeseed and improve the lodging resistance of rapeseed.

[0013] The present invention also provides the use of the plant growth regulator containing 28-epihomobrassinolide and benzylaminopurine in improving rapeseed quality, adjusting rapeseed fatty acid composition, or increasing rapeseed yield. When improving rapeseed quality, the oil content and crude protein content of rapeseed grains can be significantly increased, the glucosinolate content can be reduced, and the rapeseed quality can be effectively improved. When used to adjust the rapeseed fatty acid composition, the content of beneficial oils linoleic acid, oleic acid and linolenic acid can be effectively increased, and the content of inferior components such as erucic acid, stearic acid and palmitic acid can be reduced, which plays a role in improving the quality of rapeseed oil and provides a basis for further improving the economic benefits of rapeseed. When used to adjust rapeseed yield, the yield of rapeseed can be significantly increased, and the yield-increasing effect is extremely significant.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] The invention discloses a plant growth regulator comprising 28-epihomobrassinolide and benzylaminopurine. The invention can effectively improve the plant height, stem thickness, number of effective branches, number of effective siliques, number of effective grains, and thousand-grain weight of rapeseed after applying the plant growth regulator compounded with 28-epihomobrassinolide and benzylaminopurine to the aboveground leaf surface of rapeseed in the growing period, improve the agronomic traits of rapeseed, and provide a basis for increasing yield. In addition, the stress resistance of rapeseed can be increased, the lodging resistance of rapeseed can be improved, and the effects on improving rapeseed quality, regulating rapeseed fatty acid composition, and increasing rapeseed yield are remarkable. The plant growth regulator of the invention can significantly increase the oil content and crude protein content of rapeseed grains, reduce the content of glucosinolates, effectively improve the quality of rapeseed, and can also effectively increase the content of beneficial oils linoleic acid, oleic acid and linolenic acid, reduce the content of inferior components such as erucic acid, stearic acid and palmitic acid, play a role in improving the quality of rapeseed oil, and provide a basis for further improving the economic benefits of rapeseed. DETAILED DESCRIPTION

[0016] The technical solution of the present invention is further described in detail below in conjunction with the embodiments, but the protection scope of the present invention is not limited thereto.

[0017] Applying a compound plant growth regulator containing 28-epi-homobrassinolide and benzylaminopurine on the leaves during the rapeseed seedling stage and after transplanting can help increase the plant height, stem thickness, number of effective branches, number of effective siliques, number of effective grains and thousand-grain weight of rapeseed. Its various agronomic traits are significantly better than those of the blank treatment and the control agent. The increase in stem thickness can effectively improve the rapeseed's resistance to lodging and increase its stress resistance. After applying a composite plant growth regulator containing 28-epihomobrassinolide and benzylaminopurine, the quality of rapeseed was greatly improved, the content of inferior components such as erucic acid, stearic acid and palmitic acid was reduced, and the content of beneficial oils such as linoleic acid, oleic acid and linolenic acid was increased. The yield was increased compared with the treatment without applying the composite plant growth regulator containing 28-epihomobrassinolide and benzylaminopurine, and the oil content and crude protein content of rapeseed grains were increased, while the content of glucosinolates was reduced. The yield of the plot was significantly higher than that of the blank treatment and the control agent treatment. The yield-increasing effect reached a significant level, which can significantly increase the economic benefits of rapeseed.

[0018] 1. Experimental Drugs

[0019] Table 1 Experimental drug information

[0020] Chinese name common name Dosage form and content Production Unit 28-Epihomobrassinolide brassinolide 90%TC Yunnan Yunda Technology Agrochemical Co., Ltd. Benzylaminopurine 6-Benzylaminopurine 99%TC Jiangsu Fengyuan Bioengineering Co., Ltd.

[0021] Table 2 Comparative drug information

[0022] Chinese name Dosage form and content Production Unit 28-Epihomobrassinolide 0.004% SL Ningbo Sanjiang Yinong Chemical Co., Ltd. Benzylaminopurine 2% SL Chongqing Yiershuangfeng Technology Co., Ltd.

[0023] 2. Test methods

[0024] 1. Chemical treatment

[0025] Weigh 28-epihomobrassinolide technical and benzylaminopurine technical, dissolve them in DMF, dilute them with 0.1% Tween-80 aqueous solution, use them as mother solution for later use, and prepare drug solutions with corresponding concentrations according to requirements. 0.1% Tween-80 aqueous solution is used as blank control (CK).

[0026] 2 Experimental treatment

[0027] Except for the application of 28-epihomobrassinolide and benzylaminopurine, the same field management methods were adopted for each treatment. Seedlings were planted between 2-3 leaves, and seedlings were transplanted between 3-5 leaves. The soil was cultivated in time, and the weeding was carried out during the green period. The seedling density was 21.0×104 plants / hm 2 The experiment was carried out in a randomized block arrangement, with each treatment repeated 3 times and each plot area of ​​33.3m 2 . 2.5m wide protection rows were set around the experimental field.

[0028] 3. Application period and frequency

[0029] Application period: Rapeseed seedling stage and after transplanting;

[0030] Number of applications: 2 times;

[0031] Method of application: foliar spray.

[0032] 4 Test indicators

[0033] 4.1 Output indicators

[0034] After rapeseed matured, three representative sampling points were selected in each experimental plot, each with a length of 1 m 2 , harvest, thresh and measure yield.

[0035] 4.2 Economic traits

[0036] Three sampling points were selected in each experimental plot, and 10 plants were sampled continuously and taken back to the indoor test to measure the number of effective siliques per plant, the number of grains per silique, and the thousand-grain weight. At the time of harvest, the plots were harvested, dried, and weighed separately to calculate the yield of each plot.

[0037] 4.3 Quality Measurement

[0038] Grain samples were taken from each experimental plot, and the oil content, fatty acid composition, and glucosinolate content were measured using the NYDL-3000 intelligent multi-parameter grain and oil quality rapid tester, and the crude protein content was measured using the Kjeldahl method.

[0039] 5 Data processing

[0040] Excel 2023 software was used for data processing and calculation, and SPSS22.0 software was used for statistical analysis.

[0041] 6 Results Analysis

[0042] 6.1 Effects of 28-epihomobrassinolide and benzylaminopurine combined plant growth regulator on economic traits of rapeseed

[0043] Table 3 Effects of 28-epihomobrassinolide and benzylaminopurine combined plant growth regulator on economic traits of rapeseed (1)

[0044]

[0045] Note: Different letters in the table indicate significant differences (p<0.05), the same below.

[0046] Table 4 Effects of 28-epihomobrassinolide and benzylaminopurine combined plant growth regulator on economic traits of rapeseed (2)

[0047]

[0048]

[0049] As shown in Table 3-4, compared with the blank treatment, the treatments of spraying 28-epi-homograssinolide and benzylaminopurine compound plant growth regulator, i.e., treatment 3-27, showed significant increases in plant height, stem diameter, number of effective branches, number of effective siliques, number of effective grains, and thousand-grain weight; compared with the control agent, the treatments of spraying 28-epi-homograssinolide and benzylaminopurine compound plant growth regulator showed a trend of first increasing and then decreasing in plant height, stem diameter, number of effective branches, number of effective siliques, number of effective grains, and thousand-grain weight. Among treatments 3-27, the data of treatment 5 were the most significant, with a plant height of 198.4 cm, a stem diameter of 23.3 cm, a number of effective branches of 8.9 per plant, a number of effective horns of 379 per plant, a number of effective grains of 21.4 per horn, and a thousand-grain weight of 4.8 g, which were significantly higher than other treatments. Among them, the increase in stem diameter can effectively improve the resistance of rapeseed and play an important role in lodging resistance. In summary, the application of 28-epihomobassinolide and benzylaminopurine composite plant growth regulator can effectively improve the economic traits of rapeseed and provide a basis for increasing yield.

[0050] 6.2 Effects of 28-epihomobrassinolide and benzylaminopurine combined plant growth regulator on rapeseed quality

[0051] The effects of applying the combined plant growth regulator of 28-epihomobassinolide and benzylaminopurine on the oil content, crude protein and glucosinolate content of rapeseed are shown in Table 5.

[0052] Table 5 Effects of 28-epihomobrassinolide and benzylaminopurine combined plant growth regulator on rapeseed quality

[0053]

[0054]

[0055] As shown in Table 5, the application of 28-epihomobrassinolide and benzylaminopurine composite plant growth regulator has a significant effect on the oil content, crude protein content and glucosinolate content of rapeseed. With the increase of the application amount of 28-epihomobrassinolide and benzylaminopurine composite plant growth regulator, the oil content of rapeseed showed a trend of first increasing and then decreasing, gradually increasing from 40.3% in CK treatment to 42.77% in treatment 5 and reaching the maximum value, and then decreasing to 40.42% in 23 treatment. With the increase of the application amount of 28-epihomobrassinolide and benzylaminopurine composite plant growth regulator, the crude protein content of rapeseed showed a trend of first increasing and then decreasing. Compared with CK, it showed a continuous increase, gradually increasing from 21.7% in CK treatment to 25.59% in treatment 5, reaching the maximum value. From the perspective of glucosinolate content, its content showed a decreasing trend with the increase of the application amount of 28-epihomobrassinolide and benzylaminopurine compound plant growth regulator, and was significantly lower than that of CK treatment. In summary, the application of 28-epihomobrassinolide and benzylaminopurine compound plant growth regulator can significantly increase the oil content and crude protein content of rapeseed grains, reduce the glucosinolate content, and effectively improve the quality of rapeseed.

[0056] 6.3 Effects of 28-epihomobrassinolide and benzylaminopurine combined plant growth regulator on the fatty acid composition of rapeseed grain

[0057] Table 6 Effect of 28-epihomobrassinolide and benzylaminopurine composite plant growth regulator on the fatty acid composition of rapeseed grain

[0058]

[0059]

[0060] As shown in Table 6, the 28-epihomobrassinolide and benzylaminopurine compound plant growth regulator has a significant effect on the oleic acid, linolenic acid and stearic acid content of oleic acid seeds. With the increase of the application amount of the 28-epihomobrassinolide and benzylaminopurine compound plant growth regulator, the oleic acid content of rapeseed seeds first increased and then decreased, and was significantly higher than that of the CK treatment. Among the different treatment groups, the oleic acid, linoleic acid and linolenic acid content of rapeseed seeds was the highest in treatment 5 (oleic acid 70.89%, linoleic acid 17.49%, linolenic acid 11.38%), and the lowest in CK treatment (oleic acid 61.11%, linoleic acid 16.01%, linolenic acid 8.41%). The change trends of the mass fractions of erucic acid, stearic acid and palmitic acid in rapeseed seeds in different treatment groups were basically the same, and the overall content decreased. In summary, the application of 28-epihomobassinolide and benzylaminopurine composite plant growth regulator can effectively increase the content of beneficial oils oleic acid, linoleic acid and linolenic acid, reduce the content of inferior components erucic acid, stearic acid and palmitic acid, play a role in improving the quality of rapeseed oil, and provide a basis for further improving the economic benefits of rapeseed.

[0061] 6.4 Effect of 28-epihomobrassinolide and benzylaminopurine combined plant growth regulator on rapeseed yield

[0062] Table 7 Effect of 28-epihomobrassinolide and benzylaminopurine composite plant growth regulator on rapeseed yield

[0063]

[0064]

[0065] As shown in Table 7, in the treatments of applying the composite plant growth regulator of 28-epihomobrassinolide and benzylaminopurine, the average yield of rapeseed was significantly higher than that of the blank control group, and the yield increase rates in treatments 5-7, 10-12, 15-17, 20-21, and 25-26 were all higher than 10%, and the yield increase effect reached an extremely significant level. Among them, the yield of the plot in treatment 5 was the highest, which was 7.97kg, and the yield increase was 16.69%, which shows that the yield increase effect of applying the composite plant growth regulator of 28-epihomobrassinolide and benzylaminopurine to rapeseed was extremely significant.

[0066] 7 Conclusion

[0067] The test results show that spraying the composite plant growth regulator containing 28-epi-homobrassinolide and benzylaminopurine on the rapeseed seedling stage and after transplanting and returning to green is beneficial to increasing the plant height, stem thickness, number of effective branches, number of effective pods, number of effective grains and thousand-grain weight of rapeseed, and various agronomic traits are significantly better than those of blank treatment and control agents, and the quality is greatly improved; the yield is increased compared with the treatment without applying the composite plant growth regulator containing 28-epi-homobrassinolide and benzylaminopurine, and the yield increase effect reaches a significant level, which can significantly increase the economic benefits.

Claims

1. A plant growth regulator comprising 28-epihomobassinolide and benzylaminopurine, characterized in that: The active ingredient of the plant growth regulator is a mixture of 28-epihomobrassinolide and benzylaminopurine.

2. The plant growth regulator comprising 28-epihomobassinolide and benzylaminopurine as claimed in claim 1, characterized in that: The mass ratio of the 28-epihomobassinolide to benzylaminopurine is 1-5:124.75-1996.

3. The plant growth regulator comprising 28-epihomobassinolide and benzylaminopurine as claimed in claim 2, characterized in that: The mass ratio of the 28-epihomobrassinolide to benzylaminopurine is 1-3:249.5-1996.

4. The plant growth regulator comprising 28-epihomobassinolide and benzylaminopurine as claimed in claim 2, characterized in that: The mass ratio of the 28-epihomobassinolide to benzylaminopurine is 1:

499.

5. Use of the plant growth regulator comprising 28-epihomobassinolide and benzylaminopurine according to any one of claims 1 to 4 in regulating the growth of rapeseed.

6. Use of the plant growth regulator comprising 28-epihomobassinolide and benzylaminopurine in regulating rapeseed growth as claimed in claim 5, characterized in that: Plant growth regulators are applied to rapeseed during the seedling stage and / or after transplanting.

7. Use of the plant growth regulator comprising 28-epihomobassinolide and benzylaminopurine in regulating rapeseed growth as claimed in claim 5, characterized in that: The plant growth regulator is diluted with water to prepare a liquid with an active ingredient concentration of 1.2574-20.01 mg / L, measured by the total mass concentration of 28-epihomobassinolide and benzylaminopurine, and sprayed on the above-ground leaves of rapeseed during the rapeseed seedling stage and after transplanting.

8. Use of the plant growth regulator comprising 28-epihomobassinolide and benzylaminopurine in regulating rapeseed growth as claimed in claim 5, characterized in that: The plant growth regulator can effectively increase the plant height, stem thickness, number of effective branches, number of effective siliques, number of effective grains and thousand-grain weight of rapeseed, thereby improving the agronomic traits of rapeseed.

9. Use of the plant growth regulator comprising 28-epihomobassinolide and benzylaminopurine according to any one of claims 1 to 4 in increasing the stress resistance of rapeseed, characterized in that: The plant growth regulator can effectively increase the stem thickness of rapeseed and improve the lodging resistance of rapeseed.

10. Use of the plant growth regulator comprising 28-epihomobassinolide and benzylaminopurine according to any one of claims 1 to 4 in improving rapeseed quality, regulating rapeseed fatty acid composition, or increasing rapeseed yield.

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