Composition containing Phellodendron amurense bark extract and preparation method and application thereof

Abstract

The present invention discloses a composition containing a Phellodendron bark extract and a preparation method and application thereof, which specifically belongs to the field of daily chemical preparations; the composition containing the Phellodendron bark extract includes components: Phellodendron bark extract, Schisandra chinensis extract, Rheum palmatum extract, Kochia scoparia extract; the Kochia scoparia extract is fermented by Pleurotus eryngii and Phellodendron acutum. The present invention provides a composition containing the Phellodendron bark extract, which has excellent ability to inhibit Propionibacterium acnes and good anti-inflammatory and soothing effects; the resident skin topical agent provided by the present invention can effectively inhibit the growth of skin epidermal bacteria, reduce the occurrence of skin inflammation, promote skin stratum corneum metabolism, improve facial gloss, repair acne-damaged skin, and has significant acne-removing, antibacterial, anti-inflammatory and soothing effects.

Description

Technical Field

[0001] The invention belongs to the field of daily chemical preparations, and particularly relates to a composition containing a Phellodendron amurense bark extract and a preparation method and application thereof. Background Art

[0002] With the acceleration of modern life and the intensification of environmental pollution, skin problems have become the focus of consumers, especially acne, inflammation, bacterial infection and sensitive skin, which have brought great troubles to people's quality of life and mental health. Although traditional chemical synthetic skin care products can alleviate these symptoms to a certain extent, their potential side effects and irritation often make consumers stay away.

[0003] Natural plants are widely used in the field of skin care due to their unique biological activity and mildness. Plant extracts are usually rich in a variety of active ingredients, such as alkaloids, flavonoids, polyphenols, etc. These ingredients are excellent in inhibiting bacterial growth, reducing inflammatory responses, and promoting skin repair. However, the effect of a single plant extract is often limited, and it needs to be used in combination with multiple plant extracts to achieve a synergistic effect.

[0004] Fermented products or extracts of fermented products refer to products that are produced by converting the sugars contained in natural materials into alcohol through the fermentation and maturation process without using any other solvents, thereby enriching the effective ingredients such as flavonoids, vitamin C, vitamin P, amino acids, catechins, etc. in the natural materials, thereby improving the extraction efficiency. In addition, sometimes for natural materials that are irritating or toxic, the fermentation extraction method can be used to stabilize or reduce the toxicity of the natural materials or convert them into stable derivatives, which can greatly improve the efficacy.

[0005] The research focus of the present invention is to rationally compound a variety of natural plant extracts through scientific proportions and fermentation extraction technology to form a composition with multiple functions. This composition can directly target the cause of acne, inhibit bacterial reproduction, and reduce inflammatory reactions, thereby effectively eliminating acne. Summary of the invention

[0006] In view of the deficiencies of the prior art, the object of the present invention is to provide a plant extract composition with acne-removing, anti-inflammatory, antibacterial and soothing effects, which can directly target the cause of acne, inhibit bacterial reproduction, and reduce inflammatory reactions, thereby effectively eliminating acne.

[0007] To achieve the above object, the present invention discloses the following technical solutions:

[0008] In a first aspect, the present invention provides a composition containing a Phellodendron amurense bark extract, comprising the following components by weight:

[0009]

[0010] Furthermore, the preparation method of the Kochia scoparia fruit extract comprises the following steps:

[0011] (1) taking a seed solution of Trichoderma tricholoma and a seed solution of Pholiota capillaris and mixing them evenly to obtain a composite fungus seed solution;

[0012] (2) grinding the Kochia scoparia fruit, adding twice the weight of water after grinding, soaking at 40° C. for 1 day, and centrifuging to obtain a Kochia scoparia fruit soaking extract;

[0013] (3) Inoculating the composite bacteria seed solution into a fermentation medium at an inoculation rate of 9-12 v / v%, and adding the Kochia scoparia extract of step (2) to start fermentation culture, fermenting and culturing for 4-6 days at a temperature of 25-30° C., a rotation speed of 100-200 r / min, and a ventilation volume of 1-1.5 vvm, centrifuging, taking the supernatant and filtering to obtain a filtrate, the filtrate is concentrated under reduced pressure, and then freeze-dried to obtain a Kochia scoparia extract.

[0014] Preferably, the method for preparing the seed solution of Trichoderma trichoderma comprises:

[0015] S1. Cut off the fruiting body tissue fragments of Trichoderma tricholoma, inoculate them into PDA medium, and culture them for 8 days;

[0016] S2. Pick the mycelium from the PDA medium and inoculate it into a sterile PDA medium. After culturing at 25°C in the dark for 15 days, illuminate it for 3 days, select the strains with faster color change and neat mycelium growth and inoculate them into the seed medium. Cultivate them at a temperature of 28°C and a shaking speed of 150 r / min for 7 days to obtain the seed solution of the hairy-stalked money mushroom.

[0017] The seed culture medium is composed of glucose, peptone, beef extract, KH 2 PO 4 MgSO 4 , Vitamin B 1 and water, with a pH of 6.0.

[0018] Preferably, the method for preparing the Phellodendron acutum seed liquid comprises:

[0019] A1. Cut the fruiting body tissue fragments of Pholiota acuta, inoculate them into PDA medium, and culture them for 8 days;

[0020] A2. Pick the mycelium from the PDA medium and insert it into the sterile PDA medium. After culturing in dark for 15 days, light for 3 days, select the strain with faster color change and neat mycelium growth and inoculate it into the seed medium, and culture for 7 days to obtain the sharp scale Pholiota cap seed solution;

[0021] The seed culture medium is composed of glucose, peptone, beef extract, KH 2 PO4 MgSO 4 , Vitamin B 1 and water, with a pH of 6.0.

[0022] Preferably, in the step (1), the mass ratio of the mixture of the Trichosanthes trichosanthes seed solution and the Pholiota capillaris seed solution is 2:1.

[0023] Preferably, the fermentation medium consists of glucose, peptone, yeast extract, KH 2 PO 4 MgSO 4 , Vitamin B 1 and water, and the pH of the fermentation medium is 5.5.

[0024] In a second aspect, the present invention provides use of the composition containing the Phellodendron amurense bark extract described in the first aspect in the preparation of a skin care product with acne-removing, antibacterial, anti-inflammatory and / or soothing effects.

[0025] In a third aspect, the present invention provides a leave-on skin external preparation, comprising the composition containing the Phellodendron amurense bark extract according to the first aspect;

[0026] The amount of the composition containing the Phellodendron amurense bark extract added to the leave-on skin external preparation is ≤1 wt %.

[0027] Furthermore, the leave-on skin topical preparation also contains a moisturizer, a preservative, a thickener, a chelating agent, a pH regulator and a solvent.

[0028] In a fourth aspect, the present invention provides a method for preparing the residual skin external preparation according to the third aspect, comprising the following steps:

[0029] The moisturizer, thickener, chelating agent, preservative and solvent are stirred and mixed at a temperature of 60-90°C and uniformly dispersed, then the temperature is lowered to below 45°C and the composition containing the Phellodendron amurense bark extract is added and continued to be stirred, and finally a pH adjuster is added to adjust the pH to 6.5-7 to obtain a leave-on skin topical preparation.

[0030] In the present invention:

[0031] Phellodendron amurense bark extract is a natural plant extract ingredient derived from the bark of the Rutaceae plant Phellodendron amurense. It is rich in various alkaloids and flavonoids, mainly including berberine, phellodendronine, magnolia pine, jatrorrhizine, palmatine, etc., as well as various active ingredients such as phellodendron lactone and phellodendron ketone. These active ingredients give it excellent antioxidant, anti-inflammatory, pore-shrinking, oil-control and antibacterial effects. Berberine and other ingredients have a significant inhibitory effect on a variety of pathogens, and can directly act on the bacterial cell wall to destroy its structure, thereby inhibiting the growth and reproduction of bacteria. At the same time, antioxidant ingredients such as flavonoids can scavenge free radicals, protect cells from oxidative damage, help improve the skin microenvironment, and promote the repair and regeneration of damaged skin cells.

[0032] Schisandra extract is rich in lignans, polysaccharides and volatile oils, which can play an antioxidant role by scavenging free radicals, and also show a protective effect on the oxidative stress of skin cells. Schisandra extract has an excellent scavenging effect on DPPH, and can reduce the apoptosis of HaCaT cells induced by tert-butyl hydroperoxide (tBHP), reduce the level of reactive oxygen species (ROS) in cells, and thus effectively reduce oxidative damage. In addition, Schisandra oil can directly scavenge free radicals, reduce the oxidative damage of the skin caused by free radicals, and increase the thickness of the epidermis, more effectively protecting the skin from external stimuli and damage.

[0033] Rhubarb palmatum extract is a natural extract extracted from the Rheum genus of the Polygonaceae family. The main extracted ingredients include rhubarb and chrysophanol, which can promote blood circulation in the skin, improve skin permeability, promote the absorption of effective ingredients, and have antibacterial effects, remove acne, and astringe the skin.

[0034] Kochia fruit extract is a natural plant ingredient extracted from Kochia fruit, which has the effects of anti-oxidation, anti-inflammatory, moisturizing and soothing the skin. It is rich in polyphenolic compounds, can effectively inhibit the generation of free radicals, protect the skin from environmental pollution and ultraviolet radiation damage, in addition, Kochia fruit extract also has anti-inflammatory effects, can soothe skin discomfort and inflammatory reactions caused by environmental pollution and ultraviolet radiation. The present invention has found that the mixed fermentation of Kochia fruit crude extract using hairy-stalked money mushroom and sharp-scaled yellow umbrella can promote the effect of the overall formula of Phellodendron bark extract, Schisandra extract, palm leaf rhubarb extract and Kochia fruit extract on antibacterial, anti-inflammatory and soothing. This is because the composite fermentation process of hairy-stalked money mushroom and sharp-scaled yellow umbrella enriches the active functional ingredients in the Kochia fruit crude extract, and the fermentation products of hairy-stalked money mushroom and sharp-scaled yellow umbrella are rich in small molecule polypeptides, polysaccharides, vitamins and other active ingredients, which have antioxidant, anti-inflammatory and antibacterial effects, and also have a gain effect on the overall formula.

[0035] Beneficial effects of the present invention:

[0036] 1. The composition provided by the present invention has excellent ability to inhibit Propionibacterium acnes and good anti-inflammatory and soothing effects;

[0037] 2. The resident skin topical agent provided by the present invention can effectively inhibit the growth of bacteria in the skin epidermis, reduce the occurrence of skin inflammation, promote the turnover of the skin stratum corneum, improve facial gloss, repair acne-damaged skin, and has significant acne-removing, antibacterial, anti-inflammatory and soothing effects. DETAILED DESCRIPTION

[0038] To better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. It should be understood by those skilled in the art that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0039] The experimental methods used in the embodiments and comparative examples are conventional methods unless otherwise specified; the materials, reagents, etc. used are all commercially available unless otherwise specified; the percentages mentioned in the embodiments and comparative examples are all mass percentages unless otherwise specified.

[0040] In the present invention:

[0041] Phellodendron amurense bark extract is Phellodendron amurense extract, INCI name is PHELLODENDRON AMURENSE EXTRACT, purchased from Ningxia Vanilla Biotechnology Co., Ltd.;

[0042] Schisandra chinensis extract is Schisandra chinensis extract, INCI name is SCHIZANDRACHINENSIS EXTRACT, purchased from Youshuo Biotechnology Co., Ltd.

[0043] Rheum palmatum extract is Rheum palmatum root / stalk extract, INCI name is RHEUMPALMATUM ROOT / STALK EXTRACT, purchased from Waterless Biotechnology Co., Ltd.

[0044] Kochia fruit is the fruit part of the plant of Bassiascoparia (L.) AJScott of the Amaranthaceae family, purchased from Guangzhou Qingping Medicinal Materials Wholesale Market;

[0045] Flammulina velutipes, strain number CGMCC 3.7402, was purchased from China General Microbiological Culture Collection Center;

[0046] Pholiota squarrosoides, strain number GDMCC NO.: 5.310, purchased from Guangdong Microbiological Culture Collection Center

[0047] Other raw materials are commercially available.

[0048] Preparation of Kochia scoparia fruit extract:

[0049] Step (1), preparing seed solution:

[0050] Cut tissue fragments from the fruiting bodies of the hairy-stalked money mushroom under sterile conditions, inoculate them in a PDA medium, and culture them at a temperature of 28° C. for 8 days; pick 20 pieces of agar with mycelium from the PDA medium under sterile conditions, inoculate them into a sterile PDA medium again, culture them in the dark at 25° C. for 15 days, and then illuminate them for 3 days, select strains with faster color change and neat mycelium growth as mother strains, and then inoculate them into seed culture medium, culture them at a temperature of 28° C. and a shaking speed of 150 r / min for 7 days to obtain the hairy-stalked money mushroom seed liquid;

[0051] Cut tissue fragments from the fruiting bodies of Pholiota acuminata under sterile conditions, inoculate them in PDA medium, and culture them at a temperature of 28° C. for 8 days; pick 20 pieces of agar with mycelium from the PDA medium under sterile conditions, inoculate them into sterile PDA medium again, culture them in the dark at 25° C. for 15 days, and then illuminate them for 3 days, select strains with faster color change and neat mycelium growth as mother strains, and then inoculate them into seed medium, culture them at a temperature of 28° C. and a shaking speed of 150 r / min for 7 days to obtain the Pholiota acuminata seed solution;

[0052] The seed culture medium in step (1) is: pH = 6.0, based on its total mass fraction of 100%, composed of 2.5% glucose, 0.1% peptone, 0.2% beef extract, KH 2 PO 4 0.1%, MgSO 4 0.15%, Vitamin B 1 0.01%, the balance is water.

[0053] Step (2), grinding the Kochia scoparia fruit, adding 2 times the weight of water, soaking at 40° C. for 1 day, and centrifuging to obtain the Kochia scoparia fruit soaking extract.

[0054] Step (3), pre-mixing the seed liquid of the Psoralea corylifolia and the Pholiota adzuki bean seed liquid prepared in step (1) in a volume ratio of 2:1, inoculating the mixture into a 5L fermentation medium fermentation tank with an inoculation amount of 9-12%, adding the Kochia scoparia soaking extract of step (2) to start fermentation culture, wherein the mass ratio of the Kochia scoparia soaking extract to the composite bacteria seed liquid is 2:1, fermenting and culturing for 4-6 days at a temperature of 25-30°C, a rotation speed of 100-200r / min, and a ventilation volume of 1-1.5vvm, performing deep fermentation to obtain a mixture of edible fungi fermentation and Kochia scoparia soaking extract, centrifuging, and filtering the supernatant;

[0055] The fermentation medium in step (3) has a pH of 5.5 and is composed of 3% glucose, 0.4% peptone, 0.4% yeast extract, and KH 2 PO 4 0.15%,MgSO 4 0.15%, Vitamin B 1 0.01%, the balance is water.

[0056] Step (4), the filtrate obtained by filtering in step (3) is concentrated under reduced pressure at 60° C. to a moisture content of 8%, then freeze-dried, and crushed through a 120-mesh sieve to obtain a powder, which is the Kochia scoparia fruit extract.

[0057] The specific fermentation preparation parameters are shown in Table 1.

[0058] Table 1 Fermentation preparation parameters of Kochia scoparia extract

[0059]

[0060] In Table 1:

[0061] Kochia scoparia extract ⑤: only inoculate with the seed liquid of Trichoderma tricholoma, and the rest of the steps are consistent with the fermentation parameters of Kochia scoparia extract 1;

[0062] Kochia scoparia extract ⑥: only inoculate with Pholiota acuminata seed liquid, and the rest of the steps are consistent with the fermentation parameters of Kochia scoparia extract 1;

[0063] Kochia scoparia extract ⑦: not shown in Table 2, the preparation steps are as follows: grind the Kochia scoparia and add 2 times the weight of water, soak at 40°C for 1 day, centrifuge, spray-dry the obtained Kochia scoparia extract to obtain Kochia scoparia extract ⑦.

[0064] Preparation of a composition containing abercrombie and fitch bark extract:

[0065] The raw materials were accurately weighed according to the mass fractions in Table 2, and put into a mixing and blending machine to mix evenly to obtain a composition containing a Phellodendron amurense bark extract;

[0066] Table 2 Composition raw materials weight parts

[0067]

[0068] Note: “-” in the table means not added.

[0069] Performance Testing

[0070] In vitro inhibition test of the composition on Propionibacterium acnes:

[0071] Test sample: The composition 1-15 prepared above was diluted and dissolved with sterile water to form a test sample solution with a concentration of 0.01 wt%. Blank group: sterile water.

[0072] Test method: Inoculate the standard strain on the slant of Clostridium enrichment medium with agar powder, and culture it anaerobically at 37℃±1℃ for 48-72h; elute Propionibacterium acnes with 5mL Clostridium enrichment medium, collect the eluate in a 10mL centrifuge tube, and adjust the bacterial solution concentration to 10 by turbidimetry. 5 ~10 6 CFU / mL, use the plate method to count live bacteria; pour 18-20mL of Clostridium enrichment agar culture base into a disposable culture dish, with a pouring thickness of about 5-6mm. After the plate solidifies, aseptically absorb 100μL of Propionibacterium acnes bacterial solution on the surface of the culture medium, and quickly spread the bacterial solution evenly with a sterile coating rod; place a sterilized Oxford cup (a round stainless steel tube with an inner diameter of 6mm, an outer diameter of 8mm, and a height of 10mm) on the coated culture medium, press gently to make it contact with the culture medium without gaps, each Oxford cup is not less than 14mm from the edge of the plate, and the centers of the two Oxford cups are not less than 30mm apart. Add about 200μL of the sample to be tested to the Oxford cup, do not let it overflow, and make 3 groups of parallel for each sample to be tested, anaerobically culture at 37℃±1℃ for 48-72h, and observe the results.

[0073] Observe the transparent ring inhibition zone with naked eyes and measure the diameter of the inhibition zone with a vernier caliper. After recording 3 sets of parallel data, take the average value.

[0074] The test results are shown in Table 3.

[0075] Table 3 Antibacterial results

[0076] Group Diameter of inhibition zone (mm) Blank group 0 Composition 1 17.34 Composition 2 18.66 Composition 3 17.52 Composition 4 17.69 Composition 5 13.23 Composition 6 12.17 Composition 7 11.59 Composition 8 6.48 Composition 9 11.03 Composition 10 10.67 Composition 11 10.35 Composition 12 10.58 Composition 13 5.71 Composition 14 6.26 Composition 15 7.11

[0077] Result analysis:

[0078] According to Table 3, it can be seen that the inhibitory effect of compositions 1-4 on Propionibacterium acnes is significantly better than that of compositions 5-15, indicating that the compositions provided by the present invention have excellent ability to inhibit Propionibacterium acnes;

[0079] Compared with compositions 5-7, composition 1 has better antibacterial effect than compositions 5-7, indicating that the antibacterial effect of the overall formula is enhanced after mixed fermentation of Kochia scoparia extract, Psoralea corylifolia and Pholiotaepira acuminatum in the composition.

[0080] Inflammatory factor inhibition experiment

[0081] Samples to be tested: Take a certain amount of composition 1-15 and dilute them with physiological saline to 0.2wt%, 0.5wt%, and 1wt%, respectively.

[0082] Experimental method: RAW264.7 macrophages were used as the research object, and the cell inflammation model was established by stimulating the cells with lipopolysaccharide (LPS) (bacterial endotoxin). Macrophages were inoculated into 12-well plates and incubated in an incubator at 37°C and 5% CO. 2 After incubation for 24 h under ventilation conditions, dilutions of compositions 1-15 (0.2 wt%, 0.5 wt%, 1 wt%) were added respectively, and LPS (1 μg / mL) was added 2 h later, which was recorded as T 1 , and set up a group without adding diluent and only adding LPS, denoted as T 总 The group without LPS and only with diluent was recorded as T 0 , stimulated for 24 hours, collected the supernatant, centrifuged and tested. The ELISA kit was used to analyze the release level of the pro-inflammatory inflammatory factor TNF-α of RAW264.7.

[0083] The TNF-α inhibition rate was calculated by the following formula:

[0084] TNF-α inhibition rate (%) = [(T 总 -T 1 ) / (T 总 -T 0 )]×100%.

[0085] The higher the TNF-α inhibition rate, the better the anti-inflammatory and soothing effects of the composition. The TNF-α inhibition test results are shown in Table 4.

[0086] Table 4 TNF-α inhibition rate

[0087]

[0088] Result analysis:

[0089] According to the results in Table 4, the composition provided by the present invention has a significant inhibitory effect on the inflammatory factor TNF-α. The inhibition rates of composition 2 at concentrations of 1% (wt), 0.5% (wt), and 0.2% (wt) on the inflammatory factor TNF-α are as high as 27.661%, 24.491%, and 18.575%, respectively, having good anti-inflammatory and soothing effects.

[0090] Preparation of application examples

[0091] Weigh the raw materials according to the mass percentage in Table 5;

[0092] Step (1), weighing a certain amount of acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, xanthan gum, glycerin, allantoin and deionized water, mixing and stirring, heating to 90° C., stirring and homogenizing until completely dissolved, and keeping warm for 20 minutes to obtain phase A for use;

[0093] Step (2), weighing a certain amount of 1,3-propylene glycol, butylene glycol, 1,2-pentanediol, a preservative, a chelating agent and an emulsifier, dissolving them in distilled water to obtain phase B; cooling phase A to 60° C., adding the pre-dissolved phase B, and homogenizing and dispersing until completely mixed to obtain a mixed phase;

[0094] Step (3), cooling the mixed phase to 45°C, adding the above-prepared composition and pH adjuster in sequence, and stirring and dispersing them homogeneously until they are completely dissolved;

[0095] Step (4) cooling to 38° C., discharging the material, and obtaining the residual skin topical preparation Application Example 1-3.

[0096] Table 5 Application Example Raw Materials Mass Percentage

[0097]

[0098] Sample stability test:

[0099] 1. Heat resistance test: The constant temperature incubator was adjusted to 40°C. The application examples 1-3 prepared above were used as three samples to be tested. Three samples of each sample were placed in a transparent glass bottle with a sample volume of 20 ml / bottle. After sealing, the bottle was placed in a constant temperature incubator. After three months, the bottle was taken out and returned to room temperature to observe the changes in appearance.

[0100] 2. Cold resistance test: The constant temperature incubator was adjusted to -10℃. The application examples 1-3 prepared above were used as three samples to be tested. Three samples of each sample were put into transparent glass bottles with a sample volume of 20ml / bottle. After sealing, the bottles were placed in a constant temperature incubator. After three months, the bottles were taken out and returned to room temperature to observe the changes in appearance.

[0101] 3. Room temperature test: The application examples 1-3 prepared above are used as three samples to be tested respectively. Three tubes of each sample to be tested are placed in a transparent glass bottle with a sample volume of 20 ml / bottle. After sealing, place the bottles at room temperature for 6 months and observe the changes in the appearance of the samples.

[0102] No precipitation or settling was observed in the heat resistance test, cold resistance test, and room temperature test, and the original appearance was maintained.

[0103] Human skin patch test:

[0104] The above-prepared Application Examples 1-3 were subjected to the human skin patch test in the "Technical Specifications for Safety of Cosmetics".

[0105] The skin reaction was observed according to the standard at 30 minutes (after the indentation disappears), 24 hours and 48 hours, and the observation results were recorded (see Table 6).

[0106] Table 6 Human safety test results

[0107] Number 30 min 24h 48h Application Example 1 Grade 0, 30 people Grade 0, 30 people Grade 0, 30 people Application Example 2 Grade 0, 30 people Grade 0, 30 people Grade 0, 30 people Application Example 3 Grade 0, 30 people Grade 0, 30 people Grade 0, 30 people

[0108] Human efficacy test:

[0109] Test subjects: 30 acne patients aged 20-35 were randomly recruited, 10 in each group, and the acne patients were graded as Ⅱ. Subjects were excluded if they were pregnant or planning to be pregnant, or if they used immunosuppressants or antihistamines; if they used other products for facial acne within one month; if they had severe systemic diseases and were currently taking systemic medications, or if they were allergic to drugs.

[0110] Samples to be tested: Application Examples 1-3.

[0111] Testing process: 5 days before the test, stop using products with similar functions. After the test begins, clean your face every morning and evening, spray toner, and then use 5ml of the sample, evenly apply it on the face, and use it continuously for 28 days.

[0112] 1. Facial microbial activity

[0113] The images of the subjects' faces were collected by VISIA, and the porphyrin values ​​of the faces were analyzed using image analysis software. The values ​​on the 1st day and the 28th day were recorded.

[0114] Calculation formula for ΔT: ΔT = T 28 -T 1

[0115] T 28 : Average porphyrin value of each group on day 28;

[0116] T 1 : The average porphyrin value of each group on day 1.

[0117] 2. Skin glossiness

[0118] The subjects' faces were measured using a skin gloss meter, and the values ​​were recorded on the 1st day and the 28th day.

[0119] Calculation formula for ΔL: ΔL=L 28 -L 1

[0120] L 28 : Average glossiness of each group on the 28th day;

[0121] L 1 : Average glossiness of each group on the first day.

[0122] 3. User experience

[0123] On the 28th day, record whether the user's face feels tight, uncomfortable, or itchy after using the sample, whether the complexion is rosy and shiny, and whether acne is obvious.

[0124] The results of the human efficacy test of the samples are shown in Table 7.

[0125] Table 7 Human efficacy test results

[0126]

[0127] Test result analysis:

[0128] As can be seen from Table 7, the resident skin topical preparation provided by the present invention can effectively inhibit the growth of bacteria in the skin epidermis, reduce the occurrence of skin inflammation, promote the turnover of the skin stratum corneum, improve facial gloss, repair acne-damaged skin, and has significant acne-removing, antibacterial, anti-inflammatory and soothing effects.

[0129] Although the specific embodiments of the present invention are described above, it should be understood by those skilled in the art that this is only for illustration and the protection scope of the present invention is defined by the appended claims. Those skilled in the art may make various changes or modifications to these embodiments without departing from the principles and essence of the present invention, but these changes and modifications all fall within the protection scope of the present invention.

Claims

1. A composition containing a Phellodendron amurense bark extract, characterized in that: The composition consists of the following components in parts by mass: 1.3-2.0 parts of Phellodendron amurense bark extract; 0.3-1.0 part of Schisandra chinensis extract; 1.12-1.52 parts of Rheum palmatum extract; Kochia scoparia fruit extract 0.5-1.0 part; The preparation method of the Kochia scoparia fruit extract comprises the following steps: (1) taking the seed liquid of the tricholoma tricholoma and the seed liquid of the tricholoma acuminatum and mixing them evenly to obtain a composite fungus seed liquid; (2) Grinding the Kochia scoparia fruit, adding twice the weight of water after grinding, soaking at 40° C. for 1 day, and centrifuging to obtain a Kochia scoparia fruit soaking extract; (3) Inoculating the composite bacteria seed solution into a fermentation medium at an inoculation rate of 9-12 v / v%, and adding the Kochia scoparia extract obtained in step (2) to start fermentation culture, fermenting and culturing for 4-6 days at a temperature of 25-30° C., a rotation speed of 100-200 r / min, and a ventilation volume of 1-1.5 vvm, centrifuging, filtering the supernatant to obtain a filtrate, concentrating the filtrate under reduced pressure, and then freeze-drying to obtain a Kochia scoparia extract; In the step (1), the mass ratio of the mixture of the Psoralea corylifolia seed liquid and the Pholiotaepilla acuminata seed liquid is 2:

1.

2. The composition containing the Phellodendron chinense bark extract according to claim 1, characterized in that The preparation method of the seed liquid of Trichoderma trichoderma comprises: S1. Cut off the fruiting body tissue fragments of Trichoderma tricholoma, inoculate them into PDA medium, and culture them for 8 days; S2. Pick the mycelium from the PDA medium and inoculate it into a sterile PDA medium. After culturing at 25°C in the dark for 15 days, illuminate it for 3 days, select the strains with faster color change and neat mycelium growth and inoculate them into the seed medium. Cultivate them at a temperature of 28°C and a shaking speed of 150 r / min for 7 days to obtain the seed solution of the hairy-stalked money mushroom. The seed culture medium consists of glucose, peptone, beef extract, KH2PO4, MgSO4, vitamin B1 and water, and the pH value is 6.

0.

3. The composition containing the Phellodendron chinense bark extract according to claim 1, characterized in that The preparation method of the Pholiotaepus acuminata seed liquid comprises: A1. Cut the fruiting body tissue fragments of Pholiota acuta, inoculate them into PDA medium, and culture them for 8 days; A2. Pick the mycelium from the PDA medium and insert it into the sterile PDA medium. After culturing in dark for 15 days, light for 3 days, select the strain with faster color change and neat mycelium growth and inoculate it into the seed medium, and culture for 7 days to obtain the sharp scale Pholiota cap seed solution; The seed culture medium consists of glucose, peptone, beef extract, KH2PO4, MgSO4, vitamin B1 and water, and the pH value is 6.

0.

4. The composition containing the Phellodendron amurense bark extract according to claim 1, characterized in that The fermentation medium is prepared from glucose, peptone, yeast extract, KH2PO4, MgSO4, vitamin B1 and water, and the pH value of the fermentation medium is 5.

5.

5. Use of the composition containing the Phellodendron amurense bark extract according to any one of claims 1 to 4 in the preparation of a skin care product with acne-removing, antibacterial, anti-inflammatory and / or soothing effects.

6. A leave-on skin external preparation, characterized in that: A composition comprising a Phellodendron amurense bark extract according to any one of claims 1 to 4; The amount of the composition containing the Phellodendron amurense bark extract added to the leave-on skin external preparation is ≤1 wt %.

7. The leave-on skin external preparation according to claim 6, characterized in that: It also contains humectants, preservatives, thickeners, chelating agents, pH adjusters and solvents.

8. The method for preparing the leave-on skin external preparation according to claim 6 or 7, characterized in that: The following steps are involved: The moisturizer, thickener, chelating agent, preservative and solvent are stirred and mixed at a temperature of 60-90°C and uniformly dispersed, then the temperature is lowered to below 45°C and the composition containing the Phellodendron amurense bark extract is added and continued to be stirred, and finally a pH adjuster is added to adjust the pH to 6.5-7 to obtain a leave-on skin topical preparation.

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