A traditional Chinese medicine composition for clearing away heat, detoxifying, resisting inflammation and reducing swelling, and its preparation method and use

Abstract

The present invention discloses a Chinese medicine composition for clearing away heat and detoxifying, resisting inflammation and reducing swelling, and its preparation method and use, which belongs to the technical field of Chinese medicine. The Chinese medicine composition is made of the following raw materials in parts by weight: 25-35 parts of cardamine, 40-80 parts of dandelion, 20-60 parts of radish seeds and 20-60 parts of honeysuckle. The preparation method comprises the following steps: (1) extracting the raw materials of the formula amount by alcohol, separating the solid and liquid, and obtaining an alcohol extract and a residue; (2) extracting the residue obtained in step (1) by water, separating the solid and liquid, and obtaining a water extract; (3) combining the alcohol extract obtained in step (1) and the water extract obtained in step (2), and concentrating and drying. The present invention utilizes cardamine to compound with dandelion, radish seeds and honeysuckle, and plays a synergistic role in clearing away heat and detoxifying, resisting inflammation and reducing swelling, and has a good therapeutic effect on upper respiratory tract infections, acute and chronic pharyngitis, tonsillitis, bronchitis and fever caused by colds.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicines, and in particular to a traditional Chinese medicine composition for clearing away heat and detoxifying, resisting inflammation and reducing swelling, and a preparation method and use thereof. Background Art

[0002] Cardamine hirsuta L. is a 1-2 year old herbaceous plant of the genus Cardamine in the family Cruciferae. It grows mostly on hillsides, roadsides, wastelands and grass in cultivated land below 1,000 meters above sea level. It grows wild all over the country. It is also widely distributed in temperate regions around the world. Cardamine hirsuta is sweet, warm and non-toxic. It has the effects of relieving cough and reducing phlegm, promoting blood circulation, stopping diarrhea and reducing weight. It can also be used to treat whooping cough, chronic bronchitis, diarrhea in children, traumatic injuries and other diseases.

[0003] Liu Jiangzhou et al. (Liu Jiangzhou et al. Study on the analgesic and anti-inflammatory effects of Cardamine macrophylla water extract [J]. China Medical Herald, 2011, 27 (8): 12-14.) found in the study on the analgesic and anti-inflammatory effects of Cardamine macrophylla water extract that Cardamine macrophylla water extract can significantly prolong the time of licking the hind limbs of mice caused by the hot plate; reduce the number of twisting reactions of mice caused by acetic acid; have a significant inhibitory effect on mouse ear swelling and rat foot swelling; reduce the permeability of capillaries; and significantly inhibit the exudation of mouse peritoneal inflammation caused by acetic acid. This shows that Cardamine macrophylla has analgesic and anti-inflammatory effects. CN109303794A discloses a selenium-rich Cardamine macrophylla extract, which is extracted from the aboveground stems and leaves of Cardamine macrophylla. The invention discloses that the selenium-rich Cardamine macrophylla extract has a significant therapeutic effect in liver protection. CN109758493A discloses the use of Cardamine and its extract in the preparation of medicine for preventing or treating arrhythmia. The Cardamine and its extract can effectively prevent or treat arrhythmia and reduce the incidence of ventricular fibrillation and the duration of arrhythmia.

[0004] As a traditional Chinese herbal medicine, there is currently little research on the efficacy of Cardamine, and there is still much room for development in the medicinal value of Cardamine. Summary of the invention

[0005] The object of the present invention is to provide a Chinese medicine composition for clearing away heat and detoxifying, resisting inflammation and reducing swelling, as well as a preparation method and use thereof. The Chinese medicine composition is prepared by combining Cardamine with Taraxacum mongolicum, Radish Seed and Honeysuckle, and has the effects of clearing away heat and detoxifying, resisting inflammation and reducing swelling, and has a good therapeutic effect on upper respiratory tract infection caused by cold, acute and chronic pharyngitis, tonsillitis, bronchitis and fever.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a Chinese medicine composition for clearing away heat, detoxifying, resisting inflammation and reducing swelling, which is made from the following raw materials: cardamine, dandelion, radish seed and honeysuckle.

[0008] In some embodiments, the Chinese medicine composition comprises the following raw materials by weight: 25-35 parts of cardamine, 40-80 parts of dandelion, 20-60 parts of radish seeds and 20-60 parts of honeysuckle; preferably 28-32 parts of cardamine, 50-70 parts of dandelion, 30-50 parts of radish seeds and 30-50 parts of honeysuckle; further preferably 30 parts of cardamine, 60 parts of dandelion, 40 parts of radish seeds and 40 parts of honeysuckle.

[0009] In some embodiments, the weight ratio of Cardamine, Taraxacum officinale and Radish Seed is 3:6:4.

[0010] In a second aspect, the present invention provides a method for preparing the Chinese medicine composition, comprising the following steps:

[0011] (1) extracting the formulated amount of Cardamine, Taraxacum, Radish Seed and Honeysuckle by alcohol, separating the solid and liquid, and obtaining an alcohol extract and a residue;

[0012] (2) extracting the residue obtained in step (1) with water, separating the solid and the liquid, and obtaining a water extract;

[0013] (3) The alcohol extract obtained in step (1) and the water extract obtained in step (2) are combined, concentrated and dried to obtain the traditional Chinese medicine composition.

[0014] In some embodiments, the alcohol extraction in step (1) is: extracting at least once using 40-95% ethanol by volume; preferably, the extraction temperature is 70-80°C, and the amount of ethanol added is 5-10 times the total amount of Cardamine, Taraxacum, Radish Seed and Honeysuckle.

[0015] In some embodiments, the water extraction in step (2) is: decocting with water at least once; preferably, the amount of water added is 6-8 times the total amount of Cardamine, Taraxacum, Radish Seed and Honeysuckle.

[0016] In some embodiments, the concentration in step (3) is: concentration under reduced pressure (-0.08 to -0.09 MPa) at 50-70°C to a relative density of 1.10-1.13.

[0017] In some embodiments, the drying in step (3) is: vacuum drying, freeze drying, spray drying or air drying; preferably spray drying at 40-60°C.

[0018] The solid-liquid separation mentioned in the present invention is a conventional technical means in the art, and its purpose is to achieve separation of solid and liquid. Therefore, the present invention is not limited to its specific implementation method, and centrifugation, filtration or filter pressing can be selected according to actual needs.

[0019] In a third aspect, the present invention also provides the use of the above-mentioned Chinese medicine composition in the preparation of heat-clearing, detoxifying, anti-inflammatory and detumescent preparations.

[0020] Preferably, the heat-clearing and detoxifying, anti-inflammatory and detumescence effects include at least one of the following symptoms: upper respiratory tract infection, acute and chronic pharyngitis, tonsillitis or bronchitis.

[0021] In a fourth aspect, the present invention further provides a preparation for clearing away heat, detoxifying, resisting inflammation and reducing swelling, comprising the above-mentioned Chinese medicine composition and pharmaceutically acceptable excipients.

[0022] Preferably, the dosage form of the preparation includes oral solution, tablet, pill, capsule, granule, powder, injection or suspension.

[0023] The beneficial effects of the present invention are:

[0024] (1) The test results show that the present invention uses Cardamine, Taraxacum, Radish Seed and Honeysuckle to play a synergistic role in anti-inflammatory and swelling.

[0025] (2) The present invention selects Cardamine, dandelion, radish seed and honeysuckle to form a compound, which has good effects of clearing away heat and detoxifying, anti-inflammatory and detumescent, and has good therapeutic effects on upper respiratory tract infections caused by colds, acute and chronic pharyngitis, tonsillitis, bronchitis and fever.

[0026] (3) The present invention expands the new use of Cardamine in treating cold symptoms. DETAILED DESCRIPTION

[0027] The following examples are only used to help understand the method of the present invention and its core idea. It should be noted that for those of ordinary skill in the art, without departing from the principles of the present invention, the present invention may also be subjected to several improvements and modifications, which also fall within the scope of protection of the claims of the present invention. The following description of the disclosed embodiments enables professionals and technicians in this field to implement or use the present invention. It will be apparent to professionals and technicians in this field that various modifications to these embodiments, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but may be applied to a wider range consistent with the principles and novel features disclosed herein. Although any methods and materials similar or equivalent to those described in the present invention may be used in the implementation or testing of the present invention, preferred methods and materials are listed herein.

[0028] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

[0029] Example 1 Chinese medicine composition for clearing away heat, detoxifying, resisting inflammation and reducing swelling

[0030] The raw materials of the Chinese medicine composition are 30 parts of cardamine, 60 parts of dandelions, 40 parts of radish seeds and 40 parts of honeysuckle by weight.

[0031] The preparation method of the Chinese medicine composition is:

[0032] (1) adding 8 times the amount of 85% ethanol to the formulated amount of Cardamine, Taraxacum, Radish Seed and Honeysuckle, extracting once at 75°C for 2 hours, filtering to obtain an alcohol extract and a residue;

[0033] (2) adding 7 times the amount of water to the residue obtained in step (1), boiling once for 2 hours, filtering, and obtaining a water extract;

[0034] (3) The alcohol extract obtained in step (1) and the water extract obtained in step (2) are combined, concentrated under reduced pressure (-0.08 MPa) at 60° C. to a relative density of 1.1-1.13, and spray-dried at 60° C. to obtain the traditional Chinese medicine composition.

[0035] Example 2 Chinese medicine composition for clearing away heat, detoxifying, anti-inflammatory and detumescence

[0036] The raw materials of the Chinese medicine composition are 25 parts of cardamine, 40 parts of dandelion, 60 parts of radish seeds and 60 parts of honeysuckle by weight.

[0037] The preparation method of the Chinese medicine composition is the same as that of Example 1.

[0038] Example 3 Chinese medicine composition for clearing away heat, detoxifying, resisting inflammation and reducing swelling

[0039] The raw materials of the Chinese medicine composition are 35 parts of cardamine, 80 parts of dandelion, 20 parts of radish seeds and 20 parts of honeysuckle by weight.

[0040] The preparation method of the Chinese medicine composition is the same as that of Example 1.

[0041] Example 4 Chinese medicine composition for clearing away heat, detoxifying, and resisting inflammation and swelling

[0042] The raw materials of the Chinese medicine composition are 28 parts of cardamine, 50 parts of dandelions, 50 parts of radish seeds and 50 parts of honeysuckle by weight.

[0043] The preparation method of the Chinese medicine composition is the same as that of Example 1.

[0044] Example 5 Chinese medicine composition for clearing away heat, detoxifying, resisting inflammation and reducing swelling

[0045] The raw materials of the Chinese medicine composition are 32 parts of cardamine, 70 parts of dandelions, 30 parts of radish seeds and 30 parts of honeysuckle by weight.

[0046] The preparation method of the Chinese medicine composition is the same as that of Example 1.

[0047] Comparative Example 1: Chinese medicine composition for clearing away heat, detoxifying, anti-inflammatory and detumescent properties

[0048] The difference between this comparative example and Example 1 is that dandelion is replaced by houttuynia cordata which also has the effects of clearing away heat and detoxifying, and promoting diuresis and relieving stranguria.

[0049] Specifically: in parts by weight, the raw materials of the Chinese medicine composition are 30 parts of Cardamine, 60 parts of Houttuynia cordata, 40 parts of Radish Seeds and 40 parts of Honeysuckle.

[0050] The preparation method of the Chinese medicine composition is basically the same as that of Example 1.

[0051] Comparative Example 2: Chinese medicine composition for clearing away heat, detoxifying, anti-inflammatory and detumescent properties

[0052] The difference between this comparative example and Example 1 is that Cardamine is not contained.

[0053] Specifically: in parts by weight, the raw materials of the Chinese medicine composition are 70 parts of dandelions, 50 parts of radish seeds and 50 parts of honeysuckle.

[0054] The preparation method of the Chinese medicine composition is basically the same as that of Example 1.

[0055] Comparative Example 3: Chinese medicine composition for clearing away heat, detoxifying, anti-inflammatory and detumescent properties

[0056] The difference between this comparative example and Example 1 is that only Cardamine is used.

[0057] Specifically: by weight, the Chinese medicine composition comprises 170 parts of Cardamine.

[0058] The preparation method of the Chinese medicine composition is basically the same as that of Example 1.

[0059] Comparative Example 4: Chinese medicine composition for clearing away heat, detoxifying, anti-inflammatory and detumescent properties

[0060] The difference between this comparative example and Example 1 is that the weight ratios of the Cardamine, Taraxacum and Radish Seed are different.

[0061] Specifically: in parts by weight, the raw materials of the Chinese medicine composition are 40 parts of Cardamine, 85 parts of Taraxacum, 5 parts of Radish Seed and 40 parts of Honeysuckle.

[0062] The preparation method of the Chinese medicine composition is basically the same as that of Example 1.

[0063] 1. Evaluation of anti-inflammatory effect

[0064] 1. Test samples

[0065] The Chinese medicine compositions provided by Examples 1-5 and Comparative Examples 1-4.

[0066] 2. Test methods

[0067] KM mice (weight 20-22g, male, provided by Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.) were randomly divided into a model group and a drug administration group according to their weight, wherein the drug administration group was gavaged with the Chinese medicine composition provided in the embodiment or the comparative example, with a dosage of 50mg / kg, and the model group was gavaged with an equal volume of normal saline, once a day, for 7 consecutive days. One hour after the drug administration on the 7th day, 40μl of xylene was evenly applied to the front and back of the left ear of each group of mice, and the right ear was not applied as a control. After 6 hours of inflammation, the mice were killed by neck dislocation, the two ears were cut off, and the ear pieces were taken from the same part with a 6mm puncher and weighed. The difference in weight between the left and right ear pieces was used as the swelling degree investigation index.

[0068] The results are shown in Table 1.

[0069] Table 1 Swelling results

[0070]

[0071] Note: Different letters in the same column indicate significant differences among the groups, P < 0.05.

[0072] The results show:

[0073] Compared with the model group, the swelling of the auricles of the mice in Example 1-5 groups were significantly different (P<0.05), indicating that the Chinese medicine composition of the present invention has a good anti-inflammatory and detumescent effect on xylene-induced auricle inflammation in mice.

[0074] Compared with the model group, there was no significant difference in the swelling of the auricles of the mice in the comparative examples 1 and 3 groups (P>0.05), indicating that the Chinese medicine compositions prepared in comparative examples 1 and 3 of the present invention have no anti-inflammatory and detumescent effects on xylene-induced auricular inflammation in mice.

[0075] By comparing Example 1 with Comparative Example 2 and Comparative Example 3, it was found that in the technical solution claimed for protection by the present invention, Cardamine, Taraxacum Officinale, Radish Seed and Honeysuckle were combined to play a synergistic role in anti-inflammatory and detumescent effects.

[0076] 2. Evaluation of the effect of treating pharyngitis

[0077] 1. Test samples

[0078] The Chinese medicine compositions provided by Examples 1-5 and Comparative Examples 1-4.

[0079] 2. Test methods

[0080] SD rats (weight 180-220g, male, provided by Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.) were randomly divided into a normal group, a model group and a drug administration group according to their weight. Except for the normal group, the rats in the other groups were sprayed with 2.5% ammonia water (0.3mL / kg) into the pharynx for 14 consecutive days, twice a day, and the normal group was sprayed with an equal volume of saline. Starting from the 7th day, the drug administration group was gavaged with the Chinese medicine composition of the corresponding embodiment or comparative example, with a gavage dose of 1g crude drug / kg, once a day, and the normal group and the model group were gavaged with an equal volume of physiological saline for 7 consecutive days.

[0081] One hour after the last intragastric administration, blood was collected from the abdominal artery of the rats, and the levels of 1L-1β and TNF in the serum were determined using the kit.

[0082] The results are shown in Table 2.

[0083] Table 2 Serum 1L-1β and TNF levels (mean ± standard deviation)

[0084]

[0085] Note: Different letters in the same column indicate significant differences among the groups, P < 0.05.

[0086] The results show:

[0087] Compared with the normal group, the levels of 1L-1β and TNF in the serum of rats in the model group were significantly increased (P<0.05), indicating that the pharyngitis model was successfully established.

[0088] Compared with the model group, the 1L-1β and TNF levels in the serum of rats in Examples 1-5 were significantly reduced (P < 0.05), indicating that the Chinese medicine composition provided by Examples 1-5 of the present invention can significantly reduce the 1L-1β and TNF levels in the serum of rats with pharyngitis model, eliminate inflammatory factors, and play a role in treating pharyngitis.

[0089] Compared with the module group, there was no significant difference in the 1L-1β and TNF levels in the serum of rats in the comparative examples 1-4 groups (P>0.05), indicating that the Chinese medicine compositions provided in comparative examples 1-4 of the present invention have no effect on reducing the 1L-1β and TNF levels in the serum of rats with pharyngitis model.

[0090] 3. Evaluation of the effect of clearing heat and detoxifying

[0091] 1. Test samples

[0092] The Chinese medicine compositions provided by Examples 1-5, Comparative Example 1 and Comparative Example 4.

[0093] 2. Experimental Animals

[0094] SD rats, weighing 180-220 g, male, were provided by Beijing Weitonglihua Experimental Animal Technology Co., Ltd.

[0095] 3. Test methods

[0096] The SD rats were adaptively fed for 3 days, fasted for more than 12 hours before the experiment, and the rectal temperature of the animals was measured twice in the morning of the experiment, which was the basal body temperature. According to the basal body temperature, the SD rats were randomly divided into a model group and a drug administration group, wherein the composition group was gavaged with the Chinese medicine composition of the corresponding embodiment or comparative example, with a dosage of 50 mg / kg, and the other groups were gavaged with an equal volume of normal saline. 1 hour after the administration, 15% dry yeast (10 mL / kg) was subcutaneously injected to induce fever in each group of SD rats. After the fever, the rectal temperature of each group of SD rats was measured at 0.5h, 1h, 2h and 3h, and the temperature change value ΔT of each group of SD rats at different times after the fever was induced was calculated.

[0097] The calculation method is: ΔT=T t -T0, where T t It is the body temperature at a certain time point after fever, and T0 is the basal body temperature.

[0098] 4. Results

[0099] The results are shown in Table 3.

[0100] Table 3 Heat-clearing effect

[0101]

[0102] Note: Different letters in the same column indicate significant differences among the groups, P < 0.05.

[0103] The results show:

[0104] From the model group, we can see that after the SD rats were subcutaneously injected with yeast solution, the body temperature of the rats increased significantly, indicating that the model was successfully established.

[0105] The SD rats in Example 1-5 groups began to take effect 2 hours after intragastric administration, and the body temperature changes at 2 hours and 3 hours after administration were significantly different from those in the model group (P < 0.05); there was no significant difference in body temperature between the comparative example 1 group and the comparative example 4 group and the model group (P > 0.05), indicating that the Chinese medicine compositions provided in Examples 1-5 of the present invention have a heat-clearing effect, while the Chinese medicine compositions provided in Comparative Examples 1 and 4 have no heat-clearing effect.

[0106] The above is a further description of the present invention in conjunction with specific embodiments, but these embodiments are merely exemplary and do not constitute any limitation on the scope of the present invention. It should be understood by those skilled in the art that the details and forms of the technical solution of the present invention may be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the scope of protection of the present invention.

Claims

1. A Chinese medicine composition for clearing away heat, detoxifying, resisting inflammation and reducing swelling, characterized in that: The invention is prepared from the following raw materials in parts by weight: 25-35 parts of cardamine, 40-80 parts of dandelion, 20-60 parts of radish seeds and 20-60 parts of honeysuckle, and the weight ratio of the cardamine, dandelion and radish seeds is 3:6:

4.

2. The Chinese medicine composition according to claim 1, characterized in that: The raw materials are as follows by weight: 28-32 parts of cardamine, 50-70 parts of dandelion, 30-50 parts of radish seeds and 30-50 parts of honeysuckle.

3. The method for preparing the Chinese medicine composition according to any one of claims 1 to 2, characterized in that: The steps include: (1) extracting the formulated amount of Cardamine, Taraxacum, Radish Seed and Honeysuckle by alcohol, separating the solid and liquid, and obtaining an alcohol extract and a residue; (2) extracting the residue obtained in step (1) with water, separating the solid and the liquid, and obtaining a water extract; (3) The alcohol extract obtained in step (1) and the water extract obtained in step (2) are combined, concentrated and dried to obtain the traditional Chinese medicine composition.

4. The preparation method according to claim 3, characterized in that: The alcohol extraction in step (1) is: extracting at least once with 40-95% ethanol by volume; And / or the extraction temperature is 70-80°C.

5. The preparation method according to claim 3, characterized in that: The water extraction in step (2) is: boiling with water at least once; And / or the amount of water added is 6-8 times the total amount of Cardamine, Taraxacum and Honeysuckle.

6. Use of the Chinese medicine composition according to any one of claims 1 to 2 or the Chinese medicine composition prepared by the preparation method according to any one of claims 3 to 5 in the preparation of heat-clearing and detoxicating, anti-inflammatory and detumescent preparations.

7. A preparation for clearing away heat, detoxifying, anti-inflammatory and detumescent properties, characterized in that: The composition is prepared from the Chinese medicine composition described in any one of claims 1 to 2 or the Chinese medicine composition prepared by the preparation method described in any one of claims 3 to 5 and pharmaceutically acceptable excipients.

8. The preparation according to claim 7, characterized in that The dosage form of the preparation includes oral solution, tablet, pill, capsule, granule, powder, injection or suspension.

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