Sample analyzer and liquid discharge control method

Abstract

The embodiment of the invention provides a sample analyzer and a liquid discharge control method, and the liquid discharge control method comprises the steps: controlling a driving mechanism of the sample analyzer to drive a pipetting needle of the sample analyzer to move into a reaction container, and controlling the driving mechanism to drive the pipetting needle to perform liquid discharge operation. The liquid discharging operation at least comprises the step of discharging the first liquid, the second liquid and isolation gas located between the first liquid and the second liquid in the pipetting needle; wherein when the pipetting needle exhausts the isolation gas, the tip of the pipetting needle is located at a first position, and the height of the first position is smaller than or equal to the height of the liquid level of liquid in the reaction container when the pipetting needle exhausts the isolation gas, so that a liquid splashing area caused by bubble volley fragmentation in the liquid drainage process is greatly reduced; the needle tip pollution area of the reagent needle is controllable, so that the reagent needle is convenient to clean.

Description

Technical Field

[0001] The present application relates to the technical field of medical devices, and in particular to a sample analyzer and a liquid discharge control method. Background Art

[0002] The sample analyzer uses a pipette to add reagents and samples to the reaction container respectively, so that the reagents and samples are fully mixed to form a reaction solution. The transmittance of the reaction solution is then detected to achieve the purpose of detection, thereby determining the concentration of each component in the sample to assist in clinical diagnosis.

[0003] During the process of adding liquids such as samples and / or reagents to the reaction container with a pipette needle, if two different first liquids and second liquids are added to the pipette needle at the same time, an isolation gas is usually provided between the first liquid and the second liquid. When the pipette needle discharges liquid into the reaction container, the first liquid, the second liquid, and the isolation gas between the first liquid and the second liquid in the needle need to be discharged into the reaction container. In the prior art, the pipette needle usually discharges the first liquid, the isolation gas, and the second liquid at the same position, which results in the needle tip being relatively high from the liquid level of the reaction container when the isolation gas is discharged, usually about 10 mm.

[0004] However, if the isolation gas is discharged into the air at a higher position, bubbles may be generated at the tip of the pipette needle. If the bubbles burst, the outer wall of the pipette needle near the tip may be contaminated by small droplets produced by the bursting of the bubbles. In addition, the contamination position and size of the contaminated area are random, which is not conducive to the cleaning of the pipette needle, resulting in poor cleaning effect of the pipette needle. Ultimately, if the sample and / or reagent are added through the pipette needle with poor cleaning effect, there may be a risk of sample cross-contamination, thereby reducing the accuracy and reliability of the test results. Summary of the invention

[0005] The main purpose of the embodiments of the present application is to provide a sample analyzer and a liquid discharge control method, which aims to reduce the risk of splashing caused by the bursting of bubbles generated at the tip of the pipette needle during the process of discharging isolation gas, thereby making the contaminated area of ​​the tip of the pipette needle controllable and facilitating the cleaning of the pipette needle.

[0006] In a first aspect, an embodiment of the present application provides a sample analyzer, comprising:

[0007] A reaction device is provided with at least one reaction position, wherein the reaction position is used to place a reaction container;

[0008] A pipette needle, at least used to perform a liquid discharge operation, wherein the liquid discharge operation at least includes discharging a first liquid, a second liquid, and an isolation gas between the first liquid and the second liquid in the pipette needle;

[0009] A driving mechanism, at least used to drive the pipetting needle to perform the liquid discharging operation; and

[0010] A controller is connected to the driving mechanism and is used to:

[0011] Controlling the driving mechanism to drive the pipetting needle to move into the reaction container, and controlling the driving mechanism to drive the pipetting needle to perform a liquid discharge operation, so as to discharge the first liquid, the isolation gas, and the second liquid in the pipetting needle into the reaction container;

[0012] Wherein, when the pipette needle expels the isolation gas, the needle tip of the pipette needle is in a first position, and the height of the first position is less than or equal to the liquid level of the liquid in the reaction container when the pipette needle expels the isolation gas, or, the height of the first position is within 2 mm above the liquid level of the liquid carried by the reaction container when the pipette needle expels the isolation gas.

[0013] In a second aspect, an embodiment of the present application provides a sample analyzer, comprising:

[0014] A reaction device is provided with at least one reaction position, wherein the reaction position is used to place a reaction container;

[0015] A pipette needle, at least used to perform a liquid discharge operation, wherein the liquid discharge operation at least includes discharging a first liquid, a second liquid, and an isolation gas between the first liquid and the second liquid in the pipette needle;

[0016] A driving mechanism, at least used to drive the pipetting needle to perform the liquid discharging operation; and

[0017] A controller is connected to the driving mechanism and is used to:

[0018] Controlling the driving mechanism to drive the pipetting needle to move into the reaction container, and controlling the driving mechanism to drive the pipetting needle to perform a liquid discharge operation, so as to discharge the first liquid, the isolation gas, and the second liquid in the pipetting needle into the reaction container;

[0019] Wherein, when the pipette needle expels the isolation gas, the needle tip of the pipette needle is in a first position, and the height of the first position is less than or equal to the liquid level height of the liquid in the reaction container when the pipette needle expels the isolation gas, or, the height of the first position is within 2 mm above the liquid level of the liquid carried by the reaction container when the pipette needle expels the isolation gas.

[0020] In a third aspect, an embodiment of the present application further provides a sample analyzer, comprising:

[0021] A reaction device is provided with at least one reaction position, wherein the reaction position is used to place a reaction container;

[0022] A pipette needle, at least used to perform a liquid discharge operation, wherein the liquid discharge operation at least includes discharging a first liquid, a second liquid, and an isolation gas between the first liquid and the second liquid in the pipette needle;

[0023] A driving mechanism, at least used to drive the pipetting needle to perform the liquid discharging operation; and

[0024] A controller connected to the driving mechanism;

[0025] Wherein, the sample analyzer is provided with different first liquid discharge modes and second liquid discharge modes;

[0026] When the sample analyzer is in the first liquid discharge mode, the controller is used to:

[0027] Controlling the driving mechanism to drive the pipetting needle to move into the reaction container, and controlling the driving mechanism to drive the pipetting needle to perform a liquid discharge operation, so as to discharge the first liquid, the isolation gas, and the second liquid in the pipetting needle into the reaction container;

[0028] Wherein, the first liquid is a concentrated reagent, and when the pipette needle discharges the isolation gas, the needle tip of the pipette needle is in a first position, and the height of the first position is lower than the liquid level of the liquid carried by the reaction container when the pipette needle discharges the isolation gas, or, the height of the first position is within 2 mm above the liquid level of the liquid carried by the reaction container when the pipette needle discharges the isolation gas.

[0029] In a fourth aspect, an embodiment of the present application further provides a liquid discharge control method, which is applied to a sample analyzer, the method comprising: controlling a driving mechanism of the sample analyzer to drive a pipette needle of the sample analyzer to move into a reaction container, and controlling the driving mechanism to drive the pipette needle to perform a liquid discharge operation, wherein the liquid discharge operation at least comprises discharging a first liquid, a second liquid, and an isolation gas located between the first liquid and the second liquid in the pipette needle;

[0030] Wherein, when the pipette needle expels the isolation gas, the needle tip of the pipette needle is in a first position, and the height of the first position is less than or equal to the liquid level of the liquid in the reaction container when the pipette needle expels the isolation gas, or, the height of the first position is within 2 mm above the liquid level of the liquid carried by the reaction container when the pipette needle expels the isolation gas.

[0031] The embodiment of the present application provides a sample analyzer and a liquid discharge control method, wherein the sample analyzer is provided with a reaction device, a pipette needle, a driving mechanism and a controller. The reaction device is provided with at least one reaction position for placing a reaction container; the pipette needle is at least used to perform a liquid discharge operation, and the liquid discharge operation at least includes discharging a first liquid, a second liquid and an isolation gas between the first liquid and the second liquid in the pipette needle; the driving mechanism is at least used to drive the pipette needle to perform the liquid discharge operation; the controller is connected to the driving mechanism and is used to:

[0032] Control the driving mechanism to drive the pipette needle to move into the reaction container, and control the driving mechanism to drive the pipette needle to perform a liquid discharge operation to discharge the first liquid, the isolation gas and the second liquid in the pipette needle into the reaction container; wherein, when the pipette needle discharges the isolation gas, the needle tip of the pipette needle is in a first position, and the height of the first position is less than or equal to the liquid level height of the liquid in the reaction container when the pipette needle discharges the isolation gas, or, the height of the first position is within 2 mm above the liquid level of the liquid carried by the reaction container when the pipette needle discharges the isolation gas.

[0033] Based on the isolation gas in the pipette needle being located between the first liquid and the second liquid, the isolation gas needs to be discharged before the second liquid is discharged. In the embodiment of the present application, when the pipette needle discharges the isolation gas, the needle tip of the pipette needle is in the first position, and the height of the first position is less than or equal to the liquid level of the liquid in the reaction container when the pipette needle discharges the isolation gas, or the height of the first position is within 2 mm above the liquid level of the liquid carried by the reaction container when the pipette needle discharges the isolation gas. By discharging the isolation gas below the liquid level or near the liquid level that is less than 2 mm above the liquid level, the bubbles generated during the isolation gas discharge process are dissolved in the solution in the reaction container, or burst at the liquid level of the solution, and the bubbles burst at the liquid level have their splashing force greatly reduced under the influence of the liquid surface tension, thereby greatly reducing the splashing area caused by the shattering of bubbles in mid-air during the discharge process, so that the needle tip contamination area of ​​the reagent needle is controllable and convenient for cleaning the reagent needle.

[0034] It should be understood that the above general description and the following detailed description are merely exemplary and explanatory and cannot limit the disclosure of the embodiments of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0036] Figure 1 is a block diagram of components of a sample analyzer provided in one embodiment;

[0037] Figure 2 is a schematic structural diagram of a sample analyzer provided in one embodiment;

[0038] Figure 3 is a schematic structural diagram of a sample dispensing device of a sample analyzer in one embodiment;

[0039] Figure 4 is a schematic diagram of a first scene in which a pipette needle of a sample analyzer performs a liquid discharging operation in one embodiment;

[0040] Figure 5 is a schematic diagram of a second scene in which a pipette needle of a sample analyzer performs a liquid discharging operation in one embodiment;

[0041] Figure 6 is a schematic diagram of a third scenario in which a pipette needle of a sample analyzer performs a liquid discharging operation in one embodiment;

[0042] Figure 7 is a schematic diagram of a fourth scenario in which a pipette needle of a sample analyzer performs a liquid discharging operation in one embodiment;

[0043] Figure 8 This is a flow chart of a liquid discharge control method provided by one implementation. DETAILED DESCRIPTION

[0044] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0045] In the description of this application, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0046] The flowcharts shown in the accompanying drawings are only examples and do not necessarily include all the contents and operations / steps, nor must they be executed in the order described. For example, some operations / steps may also be decomposed, combined or partially merged, so the actual execution order may change according to actual conditions.

[0047] Some implementation methods of the present application are described in detail below in conjunction with the accompanying drawings. In the absence of conflict, the features in the following embodiments and implementation methods can be combined with each other.

[0048] Please refer to Figure 1 The present application provides a sample analyzer 100 for analyzing a sample to be tested to obtain a corresponding analysis result.

[0049] In some embodiments, the sample analyzer includes, but is not limited to, at least one of the following: a biochemical analyzer, an immunoassay analyzer, a coagulation analyzer, and a urine analyzer.

[0050] like Figure 1 As shown, the sample analyzer 100 includes a dispensing device 10 , a sample supply device 20 , a reagent supply device 30 , a reaction device 40 and a detection device 50 .

[0051] The sample supply device 20 is used to provide samples to be tested, the reagent supply device 30 is used to provide reagents that react with the samples, and the reaction device 40 is provided with at least one reaction position, which is used to place a reaction container 4011 (see Figure 2 ), the reaction container includes but is not limited to a reaction cup. The dispensing device 10 is used to inject the sample provided by the sample supply device 20 and the reagent provided by the reagent supply device 30 into the reaction container so that the sample and the reagent are mixed to form a reaction liquid. The detection device 50 is used to detect the reaction liquid in the reaction container 4011 to obtain a corresponding detection result.

[0052] See also Figures 2 to 3In some embodiments, the sample supply device 20 may include a sample delivery module (SDM) and a front track; the sample supply device 20 may also be a sample tray, which includes a plurality of sample positions such as sample tubes. The sample tray can dispatch samples to corresponding positions by rotating its tray components, for example, dispatching samples to positions where the dispensing device 10 absorbs samples. The dispensing device 10 is used to absorb samples and discharge them into the reaction container 4011 to be loaded with samples.

[0053] In some embodiments, the dispensing device 10 includes a sample dispensing device 10a, wherein the sample dispensing device 10a is used to absorb the sample supplied by the sample supply device 20 and move the sample to a preset position, such as the sample dispensing device 10a absorbs the sample from the sample supply device 20 and discharges it into the reaction container 4011 to be loaded with the sample.

[0054] The sample dispensing device 10a includes a sample needle 101 and a first driving mechanism 104, wherein the sample needle 101 is used to perform a sample suction operation or a sample discharge operation. The first driving mechanism 104 is used to drive the sample needle 101 to perform a sample suction operation or a sample discharge operation.

[0055] Optionally, the first driving mechanism 104 includes a first moving component 102 and a first power component 103. The first moving component 102 is used to drive the sample needle 101 to move in two-dimensional or three-dimensional space. For example, the sample needle 101 is driven by the first moving component 102 to perform two-dimensional or three-dimensional movement in space, thereby driving the sample needle 101 to move to absorb the sample carried by the sample supply device 20.

[0056] The first power component 103 is used to quantitatively absorb the sample through the sample needle 101. For example, the sample to be tested is a blood sample to be tested. The sample needle 101 is driven by the first moving component 102 to move to the sample tube containing the blood sample carried on the sample supply device 20, and absorbs the blood sample to be tested under the drive of the first power component 103, and transports the blood sample to be tested to the reaction container 4011 of the reaction device 40, so that the blood sample to be tested absorbed by the dispensing device 10 and the reagent provided by the reagent supply device 20 are mixed in the reaction container 4011 to prepare a reaction solution.

[0057] like Figure 3As shown, in some embodiments, the first movable assembly 102 includes a support frame 1021, and the support frame 1021 is fixed on a support rod 1022, and the support rod 1022 can move vertically and rotate. The support frame 1021 is driven by the support rod 1022 to achieve vertical movement and horizontal rotation. The sample needle 101 is set on the support frame 1021, and can reach the target position under the drive of the support frame 1021. Exemplarily, the first movable assembly 102 also includes a driver 1023 for driving the support rod 1022 to move, such as a stepping motor, but of course it is not limited to this. Optionally, the sample needle 101 is detachably connected to the first movable assembly 102, or fixedly connected.

[0058] In some embodiments, the first power component 103 includes a pipeline 1031 and a power unit 1033, wherein the pipeline 1031 is used to transport fluid medium, one end of the pipeline 1031 is connected to the sample needle 101, and the other end is connected to the power unit 1033, so that the flow direction of the fluid medium in the pipeline 1031 is changed under the action of the power unit 1033, so that the sample needle 101 can transfer the sample, wherein the power unit 1033 includes but is not limited to a pump and a syringe.

[0059] In some embodiments, the dispensing device 10 also includes a reagent dispensing device 10b, and the reagent supply device 30 includes a reagent carrying component 301 for carrying the reagent. The reagent dispensing device 10b of the dispensing device 10 absorbs the reagent carried by the reagent supply device 30 and provides it to a reaction container placed in the reaction device 40, so as to add the reagent to the reaction container to be loaded with the reagent, wherein the reagent includes but is not limited to a colorimetric reagent, a diluent, a substrate solution, an enzyme-labeled reagent, etc.

[0060] In some embodiments, the reagent carrying component 301 may be a reagent disk, which is a disk-shaped component having multiple positions for carrying reagent containers. The reagent carrying component 301 can rotate and drive the reagent container it carries to rotate, and is used to rotate the reagent container to a specific position, such as a position where the reagent is sucked by the reagent dispensing device 10b. The number of the reagent carrying components 301 may be one or more.

[0061] In some embodiments, the reagent dispensing device 10b includes a reagent needle and a second driving mechanism, wherein the reagent needle is used to perform a reagent aspiration operation or a reagent discharge operation. The second driving mechanism is used to drive the reagent needle to perform a reagent aspiration operation or a reagent discharge operation.

[0062] Optionally, the second driving mechanism has the same structure as the first driving mechanism 104, that is, the second driving mechanism includes a second moving assembly and a second power assembly. The reagent needle is driven by the second moving assembly to perform two-dimensional or three-dimensional movement in space, so that the reagent needle can move and cooperate with the second power assembly to absorb the reagent carried by the reagent carrying component 301, and move to the reaction container 4011 to be added with the reagent, and discharge the reagent into the reaction container 4011.

[0063] In some embodiments, the second moving assembly and the first moving assembly 102 have the same structure, which will not be described in detail herein.

[0064] In some embodiments, the second power assembly and the first power assembly 103 have the same structure, which will not be described in detail herein.

[0065] In some embodiments, the reagent dispensing device 10b does not add reagents through a reagent needle, but adds the reagents in the reagent tube to the reaction container 4011 through a dedicated pipeline. In this type of embodiment, there is only a sample needle 101, but no reagent needle.

[0066] It can be understood that, depending on the different body fluids to be tested and the different test items, there are different ways to add samples and reagents. For example, both samples and reagents can be added using the sample needle 101, or the sample is added using the sample needle 101 and the reagent is added using the reagent needle, or only the sample is added using the sample needle 101 and the reagent is added using other ways. That is, the sample dispensing device 10a of the dispensing device 10 is used for both sample transfer and reagent transfer; or the sample dispensing device 10a of the dispensing device 10 is used for sample transfer and the reagent dispensing device 10b is used for reagent transfer; or the sample dispensing device 10a of the dispensing device 10 is used for sample transfer and the reagent is connected to the reagent container carrying the reagent through a dedicated pipeline to add the reagent to the reaction container 4011.

[0067] Therefore, the sample needle 101 and / or the reagent needle are also referred to as a pipetting needle, that is, the pipetting needle includes at least one of the sample needle 101 and the reagent needle. The sample needle 101 performs a sample aspiration operation or a discharge operation, or the reagent needle performs a reagent aspiration operation or a discharge operation, which can be referred to as the pipetting needle performing a pipetting operation, and the pipetting operation includes a pipetting operation and a discharge operation. That is, the aspiration operation includes a reagent aspiration operation and / or a sample aspiration operation, and the discharge operation includes a reagent discharge operation and / or a sample discharge operation.

[0068] In some embodiments, the sample supply device 20 and the reagent supply device 30 constitute a liquid supply device, that is, the liquid supply device includes the sample supply device 20 for providing a sample and the reagent supply device 30 for providing a reagent.

[0069] In some embodiments, the reaction device 40 has a carrying portion 401, and the carrying portion 401 is provided with at least one reaction position for placing a reaction container 4011, the reaction container 4011 is used to receive the sample supplied by the sample supply device 20 and the reagent supplied by the reagent supply device 30, and provide a reaction place for the sample and the reagent to mix to form a reaction liquid, and the reaction device 40 is also used to incubate the reaction liquid in the reaction container 4011.

[0070] For example, the supporting portion 401 of the reaction device 40 may be a reaction disk, such as Figure 2 As shown, it is arranged in a disc-shaped component, having one or more reaction positions for placing reaction vessels 4011. The reaction disc can rotate under the drive of a third driving mechanism and drive the reaction vessels 4011 placed therein to rotate, so as to schedule the reaction vessels 4011 in the reaction disc and incubate the reaction liquid in the reaction vessels 4011.

[0071] It is understood that the reaction position can be not only arranged on the reaction disk of the reaction device 40, but also arranged independently of the reaction disk of the reaction device 40. The reaction position being arranged independently of the reaction disk means that the arrangement of the reaction position does not interfere with the rotation of the reaction disk itself.

[0072] In some embodiments, the detection device 50 is used to measure the reaction solution in the reaction container or the reaction solution after incubation to obtain the reaction data of the sample. For example, the detection device 50 includes a photometric mechanism, which is used to detect the luminescence intensity of the reaction solution in the reaction container and calculate the concentration of the component to be measured in the sample through a calibration curve.

[0073] In other embodiments, the detection device 50 includes an electrical detection mechanism (eg, an impedance detection mechanism) or a detection mechanism based on other principles (eg, an imaging detection mechanism).

[0074] Optionally, the detection device 50 is separately disposed on the peripheral side of the reaction device 40 .

[0075] In some embodiments, the sample analyzer 100 further includes at least one cleaning device 60, and the cleaning device 60 is used to clean the target device, wherein the target device includes but is not limited to a sample needle, a reagent needle, and a reaction container.

[0076] In some embodiments, the sample analyzer 100 also includes a controller 70, which is communicatively connected with the dispensing device 10, the sample supply device 20, the reagent supply device 30, the reaction device 40 and the detection device 50 to control at least one of the dispensing device 10, the sample supply device 20, the reagent supply device 30 and the reaction device 40 to complete a preset operation, such as controlling the dispensing device 10 to complete the transfer and dispensing of samples and / or reagents, or controlling the reaction device 40 to complete the incubation of the reaction liquid, or controlling the dispensing device 10, the sample supply device 20, the reagent supply device 30, the reaction device 40 and the detection device 50 to cooperate to complete the detection of the sample to be tested.

[0077] It is understood that the controller 70 may be one or more and may be arranged to control any one of the dispensing device 10, the sample supply device 20, the reagent supply device 30, the reaction device 40 and the detection device 50, or at least two of them. Alternatively, the controller 70 may be arranged independently, which is not limited here.

[0078] In some embodiments, the controller 70 includes at least a processor 701, a memory 702, a communication interface (not shown), and an I / O interface (not shown). The processor 701, the memory 702, the communication interface, and the I / O interface communicate via a bus. The processor 701 may be a central processing unit (CPU), and the processor may also be other general-purpose processors, digital signal processors (DSP), application-specific integrated circuits (ASIC), field-programmable gate arrays (FPGA), or other programmable logic devices, discrete gates or transistor logic devices, discrete hardware components, etc. Among them, the general-purpose processor may be a microprocessor or the processor may also be any conventional processor, etc.

[0079] The memory 702 is equipped with various computer programs such as operating systems and applications for the processor 701 to execute and the data required for executing the computer programs. During the analysis of the sample to be tested, if there is data that needs to be stored locally, it can be stored in the memory 702. The I / O interface includes but is not limited to serial interfaces such as USB, IEEE1394 or RS-232C, parallel interfaces such as SCSI, IDE or IEEE1284, and analog signal interfaces composed of D / A converters and converters. An input component is connected to the I / O interface, and the user can use the input component to directly input data to the controller 70. The input component includes but is not limited to a keyboard, a mouse, a touch screen or a control button. The display component can be connected to the controller 70 through the I / O interface to communicate with the controller 70 to provide relevant information prompts. The communication interface can be an interface of any communication protocol currently known, and the communication interface communicates with the outside world through a network. The controller 70 can transmit data between any components connected through the network with a preset communication protocol through the communication interface.

[0080] In some embodiments, the controller 70 is used to implement the following control method steps, specifically, the processor 701 of the controller 70 calls the computer program in the memory 702 to implement the following method steps:

[0081] Controlling the driving mechanism to drive the pipette needle to move into the reaction container, and controlling the driving mechanism to drive the pipette needle to perform a liquid discharge operation, so as to discharge the first liquid, the isolation gas and the second liquid in the pipette needle into the reaction container;

[0082] When the pipette needle expels the isolation gas, the needle tip of the pipette needle is in a first position, and the height of the first position is less than or equal to the liquid level of the liquid in the reaction container when the pipette needle expels the isolation gas, or, the height of the first position is within a preset height range above the liquid level of the liquid carried by the reaction container when the pipette needle expels the isolation gas.

[0083] Optionally, the preset height is less than or equal to 2 mm, preferably, the preset height is less than or equal to 1 mm. That is, when the pipette needle discharges the isolation gas, the tip of the pipette needle is located within a height of 2 mm above the liquid level of the liquid carried by the reaction container at that time, preferably, when the pipette needle discharges the isolation gas, the tip of the pipette needle is located within a height of 1 mm above the liquid level of the liquid carried by the reaction container at that time.

[0084] Optionally, the inner diameter of the needle port of the pipette needle for aspirating or discharging liquid is d, and the height of the first position is within a range of less than or equal to 2d above the liquid surface of the liquid carried by the reaction container when the pipette needle discharges the isolation gas. Optionally, the first liquid includes a concentrated reagent and the second liquid includes a diluent.

[0085] Exemplarily, for ease of explanation, the pipetting needle is taken as a reagent needle in the embodiments of the present application for explanation, but the pipetting needle is not limited to being only a reagent needle.

[0086] like Figure 4 As shown, the reagent needle carries a first volume of a first liquid a, a second volume of an isolation gas b and a second liquid c, wherein the first liquid a is located at the tip of the reagent needle, the second liquid c is located at the end of the reagent needle away from the tip, and the isolation gas b is located between the first liquid a and the second liquid b. The reagent needle performs a liquid discharge operation, which at least includes discharging the first liquid a, the second liquid c and the isolation gas b located between the first liquid a and the second liquid c in the reagent needle.

[0087] That is, when the reagent needle performs the liquid discharge operation, the first volume of the first liquid a, the second volume of the isolation gas b, and the third volume of the second liquid c in the reagent needle are sequentially discharged from the reagent needle, wherein the liquid volume of the second liquid c corresponding to the third volume can be set according to the detection item requirements of the sample analyzer. That is, the liquid volume corresponding to the third volume of the second liquid c discharged from the reagent needle can be set according to the detection item requirements.

[0088] Especially in the scenario where the first liquid a is a concentrated reagent and the second liquid c is a diluent, since the concentrated reagent is discharged first, after the first volume of concentrated reagent carried in the reagent needle is discharged, the isolation gas discharge is performed. Since there is still residual concentrated reagent at the tip of the reagent needle, bubbles may be generated in the process of discharging the isolation gas head. When the bubbles break, small droplets of concentrated reagent are randomly scattered on the outer surface of the reagent needle, which may cause reagent crystals on the outer surface of the reagent needle and is difficult to clean.

[0089] When the reagent needle performs the diluent discharge operation, the discharge volume of the diluent is determined according to the dilution ratio of the test item to the concentrated reagent.

[0090] Therefore, in the embodiment of the present application, during the liquid discharge process of the reagent needle, the controller 70 controls the second driving mechanism to drive the reagent needle to move into the reaction container 4011, and controls the driving mechanism to drive the reagent needle to perform the liquid discharge operation, so as to discharge the first liquid a (such as concentrated reagent), isolation gas b and second liquid c (such as diluent) in the reagent needle into the reaction container 4011; wherein, when the reagent needle discharges the isolation gas b, the needle tip of the reagent needle is in a first position, and the height of the first position is H1, and H1 is less than or equal to the liquid level height of the liquid in the reaction container when the reagent needle discharges the isolation gas b.

[0091] When the reagent needle discharges the isolation gas b, the height of the reagent needle tip is less than or equal to the liquid level of the liquid in the reaction container 4011 when the reagent needle discharges the isolation gas b, so that the reagent needle discharges the isolation gas b below the liquid surface or above the liquid surface, so that the bubbles generated during the discharge of the isolation gas b are dissolved in the solution in the reaction container 4011, or burst at the liquid surface of the solution, and the splashing force of the bubbles burst at the liquid surface is greatly reduced under the influence of the liquid surface tension, thereby greatly reducing the splashing area caused by the bubble shattering in mid-air, so that the contaminated area of ​​the reagent needle tip can be controlled for easy cleaning of the reagent needle.

[0092] like Figure 5 As shown, during the liquid discharge process of the reagent needle, the controller 70 controls the second driving mechanism to drive the reagent needle to move into the reaction container 4011, and controls the driving mechanism to drive the reagent needle to perform the liquid discharge operation to discharge the first liquid a, the isolation gas b and the second liquid c in the reagent needle into the reaction container 4011. When the reagent needle discharges the isolation gas b, the needle tip of the reagent needle is in the first position, the first position is above the liquid surface, and the position height of the first position is H1. When the reagent needle discharges the isolation gas b, the liquid level height of the liquid carried by the reaction container 4011 is H0. At this time, the distance between the needle tip of the reagent needle and the liquid surface is less than the preset height D, D≤2mm, preferably, D≤1mm. That is, H1>H0, and H1-H0<D.

[0093] Because the distance between the reagent needle tip and the liquid surface is less than the preset height D when the reagent needle discharges the isolation gas b, the distance between the bubble and the liquid surface is small, so that the bubble may contact the liquid surface as it gradually grows larger, and the splashing force of the bubble is greatly reduced under the influence of the liquid surface tension, thereby greatly reducing the splashing area caused by the bubble breakage, making the contaminated area of ​​the reagent needle tip controllable and facilitating the cleaning of the reagent needle.

[0094] Alternatively, when the reagent needle is discharging the isolation gas b, the distance between the needle tip of the reagent needle and the liquid surface is less than a preset height D, D≤2d, d is the inner diameter of the needle hole of the reagent needle, and based on the small distance between the bubble and the liquid surface, the bubble may contact the liquid surface as it gradually grows larger, and the splashing force of the bubble is greatly reduced under the influence of the liquid surface tension, thereby greatly reducing the splashing area caused by the bubble breakage, making the contaminated area of ​​the needle tip of the reagent needle controllable and facilitating the cleaning of the reagent needle.

[0095] In some embodiments, when the pipetting needle completes the discharge of the second liquid into the reaction container, the needle tip height of the pipetting needle is less than or equal to the liquid level height of the liquid in the reaction container.

[0096] See also Figure 4 and Figure 5, exemplarily, in the embodiment of the present application, completing the discharge of the second liquid c means that the reagent needle discharges the third volume of the second liquid c into the reaction container 4011 according to the requirements of the detection item. After the discharge of the third volume of the second liquid c is completed, the needle tip height of the reagent needle is less than or equal to the liquid level height of the liquid in the reaction container 4011 at this time, that is, after the discharge of the second liquid c is completed, the needle tip of the reagent needle is below the liquid surface of the solution in the reaction container 4011, or the needle tip of the reagent needle just contacts the liquid surface of the solution in the reaction container 4011, so as to avoid the reagent needle in the process of discharging liquid. Due to insufficient positioning accuracy of the distance between the reagent needle and the reaction container, the reagent needle may cause unstable pressure during discharge, unstable overshoot and back suction, thereby causing liquid hanging on the needle tip of the reagent needle, which ultimately affects the accuracy and precision of the results of the sample analysis test.

[0097] In some embodiments, during the liquid discharging operation performed by the pipette needle, the height of the discharge position of the second liquid is greater than the height of the discharge position of the isolation gas.

[0098] Optionally, during the process of the pipetting needle performing the liquid discharge operation, the height of the discharge position of the isolation gas is greater than or equal to the height of the discharge position of the first liquid.

[0099] Exemplarily, during the liquid discharge operation of the reagent needle, under the premise of satisfying the aforementioned isolation gas discharge height, the position height corresponding to the start discharge position of the first liquid a is less than or equal to the position height corresponding to the start discharge position of the isolation gas b. And / or, the position height corresponding to the start discharge position of the second liquid c is greater than the position height corresponding to the start discharge position of the isolation gas b.

[0100] In some embodiments, the height of the discharge position of the second liquid is determined according to the amount of the second liquid to be discharged by the pipette needle. Alternatively, the height of the discharge position of the second liquid carried in the needle body of the pipette needle is determined according to the amount of the second liquid carried in the pipette needle.

[0101] Exemplarily, the position at which the reagent needle discharges the second liquid c is set according to the amount of second liquid c to be discharged, so that the discharge position of the second liquid c from the reagent needle meets preset requirements, so as to avoid splashing during the discharge of the second liquid c due to the starting discharge position of the second liquid c being too high, or to avoid contamination caused by a larger area of ​​the outer surface of the reagent needle being soaked by the solution in the reaction container after the discharge of the second liquid c is completed due to the discharge position of the second liquid c being too low.

[0102] In some embodiments, the volume of the isolation gas carried in the needle body of the pipette needle is determined according to the amount of the second liquid in the needle body of the pipette needle.

[0103] like Figure 4 As shown, exemplarily, based on the isolation gas position b between the first liquid a and the second liquid c, the larger the amount of the second liquid c carried in the reagent needle, the greater the pressure on the isolation gas b may be, and the stronger the compression force on the isolation gas b. Therefore, in order to enable the isolation gas b to achieve the isolation effect of the first liquid a and the second liquid c, the volume of the isolation gas b is positively correlated with the amount of the second liquid c carried in the reagent needle, that is, the larger the amount of the second liquid c carried in the reagent needle, the larger the volume of the isolation gas b.

[0104] In some embodiments, the sample analyzer further comprises a liquid supply device for providing at least a first liquid, and before controlling the driving mechanism to drive the pipette needle to move into the reaction container, the controller is further configured to:

[0105] Controlling the driving mechanism to perform a liquid aspiration operation on the liquid supply device to aspirate the first liquid into the pipetting needle;

[0106] Wherein, the controller is also used to control the driving mechanism to drive the pipette needle to inhale the isolation gas before performing the liquid aspiration operation.

[0107] Exemplarily, the liquid supply device includes a sample supply device 20 for providing a sample and a reagent supply device 30 for providing a reagent, and the first liquid includes a sample liquid and / or a reagent.

[0108] For the convenience of explanation in this application, an example is taken in which the supply device is the reagent supply device 30, the first liquid is the concentrated reagent, and the second liquid is the diluent.

[0109] Before the reagent needle performs the discharge operation into the reaction container, the controller 70 controls the second driving mechanism to drive the reagent needle to inhale the isolation gas, and controls the second driving mechanism to drive the reagent needle to perform the aspiration operation toward the reagent supply device 30 to inhale the first liquid a (concentrated reagent) into the pipetting needle.

[0110] The second liquid c (diluent) can be sucked into the reagent needle by the second driving mechanism before the isolation gas is sucked in, or the second liquid c can be delivered to the reagent needle by the reagent supply device 30 before the isolation gas is sucked in. Alternatively, the second liquid c can be delivered to the reagent needle by the reagent supply device 30 after the isolation gas is sucked in, so that the isolation gas is located between the first liquid a and the second liquid c in the reagent needle.

[0111] For example, the reagent needle is a hollow structure, and has a liquid inlet and a liquid outlet, the liquid outlet is located at the needle tip, and the liquid inlet is connected to the reagent supply device 30 and the second drive mechanism through a pipeline. Before inhaling the isolation gas, the controller 70 controls the reagent supply device 30 to deliver a preset amount of the second liquid c to the reagent needle. After the second liquid c is delivered, the controller 70 controls the second drive mechanism to drive the reagent needle to inhale a preset volume of isolation gas b, and controls the second drive mechanism to drive the reagent needle to inhale a fixed amount of the first liquid a into the reagent supply device 30, so that the isolation gas b is located between the first liquid a and the second liquid c in the reagent needle.

[0112] In some embodiments, the driving mechanism includes a moving component for driving the pipetting needle to move, and a power component for providing the pipetting needle with a pipetting power; during the process of the pipetting needle performing the discharge operation, the controller is further used to:

[0113] Controlling the power assembly to drive the pipette needle to discharge the first liquid and the isolation gas into the reaction container;

[0114] During the process of discharging the isolation gas or after the isolation gas is discharged, the moving component is controlled to drive the pipetting needle to lift and the power component is controlled to drive the pipetting needle to discharge the second liquid into the reaction container.

[0115] Exemplarily, taking the pipette needle as a reagent needle as an example, the controller 70 drives the reagent needle to move in a two-dimensional or three-dimensional space by controlling the second moving mechanism, and provides the reagent needle with liquid suction or liquid discharge power by controlling the second power component.

[0116] When it is necessary to control the reagent needle to discharge liquid into the reaction container 4011, the controller controls the second moving mechanism to drive the reagent needle to move into the reaction container 4011, and controls the second power component to drive the reagent needle to discharge the first liquid a and isolation gas b in the reagent needle into the reaction container 4011.

[0117] During the process of discharging the isolation gas b or after the isolation gas b is discharged, the second moving component is controlled to drive the reagent needle to lift and the second power component is controlled to drive the reagent needle to discharge the second liquid c into the reaction container 4011 .

[0118] When the reagent needle discharges the isolation gas b, the needle tip of the reagent needle is at the first position, the height of the first position is H1, and H1 is less than or equal to the liquid level height of the liquid in the reaction container 4011 when the reagent needle discharges the isolation gas b.

[0119] Alternatively, the height of the first position is within a preset height range D above the liquid level of the liquid carried by the reaction container 4011 when the reagent needle expels the isolation gas b, that is, the liquid level height of the liquid carried by the reaction container 4011 when the reagent needle expels the isolation gas b is H0, H1-H0<D.

[0120] That is, the height of the position at which the reagent needle starts to discharge the second liquid c is greater than the height of the position at which the reagent needle starts to discharge the isolation gas b.

[0121] In some embodiments, during the process in which the pipette needle performs the liquid discharging operation, the controller is further configured to:

[0122] Controlling the moving assembly to drive the pipetting needle to move into the reaction container, and when the needle tip of the pipetting needle is at the first position, controlling the power assembly to drive the pipetting needle to discharge the first liquid and the isolation gas in the needle body of the pipetting needle;

[0123] After the isolation gas is discharged, the moving component is controlled to drive the pipette needle to rise to a second position, and at the second position, the power component is controlled to drive the pipette needle to discharge the second liquid in the needle body of the pipette needle, and the position height of the second position is greater than the position height of the first position.

[0124] See also Figure 4 or Figure 5 , exemplarily, when it is necessary to control the reagent needle to discharge liquid into the reaction container 4011, the second moving component is controlled to drive the reagent needle to move into the reaction container 4011, and when the needle tip of the reagent needle is at the first position, the second power component is controlled to drive the reagent needle to discharge the first liquid a and the isolation gas b in the needle body of the reagent needle. The height of the first position is H1, which is less than or equal to the liquid level height of the liquid in the reaction container when the reagent needle discharges the isolation gas b.

[0125] Alternatively, the height of the first position is within a preset height range D above the liquid level of the liquid carried by the reaction container 4011 when the reagent needle expels the isolation gas b, that is, the liquid level height of the liquid carried by the reaction container 4011 when the reagent needle expels the isolation gas b is H0, H1-H0<D.

[0126] After the isolation gas b is exhausted, the second moving component is controlled to drive the reagent needle to rise to the second position, and the second power component is controlled at the second position to drive the reagent needle to begin to exhaust the second liquid c in the needle body of the reagent needle, and the position height of the second position is greater than the position height of the first position. For example, the position height of the first position is H1, and the position height of the second position is H2, and H2>H1.

[0127] Optionally, when the reagent needle completes the discharge operation of the second liquid c, the needle tip of the reagent needle is also in the second position.

[0128] Optionally, when the reagent needle completes the discharge operation of the second liquid c, the needle tip height of the reagent needle is less than or equal to the liquid level height of the reaction container at that time.

[0129] In some embodiments, during the process in which the pipette needle performs the liquid discharging operation, the controller is further configured to:

[0130] Controlling the moving assembly to drive the pipetting needle to move into the reaction container, and when the needle tip of the pipetting needle is at the third position, controlling the power assembly to drive the pipetting needle to discharge the first liquid in the needle body of the pipetting needle;

[0131] After completing the discharge of the first liquid, the moving component is controlled to drive the pipette needle to rise, and during the lifting process of the pipette needle, the power component is controlled to drive the pipette needle to discharge the isolation gas and the second liquid in the needle body of the pipette needle into the reaction container; wherein, when the pipette needle discharges the isolation gas, the needle tip of the pipette needle is in the first position, and the position height of the third position is lower than the position height of the first position.

[0132] See also Figure 6 When it is necessary to control the reagent needle to discharge liquid into the reaction container 4011, the second moving component is controlled to drive the reagent needle to move into the reaction container 4011, and when the needle tip of the reagent needle is in the third position, the second power component is controlled to drive the reagent needle to discharge the first liquid a in the needle body of the reagent needle.

[0133] After the first liquid a is discharged, the second moving component is controlled to drive the reagent needle to rise, and during the raising process of the reagent needle, the second power component is controlled to drive the reagent needle to discharge the isolation gas b and the second liquid c in the needle body of the reagent needle into the reaction container 4011; wherein, when the reagent needle begins to discharge the isolation gas b, the needle tip of the reagent needle is in the first position, the position height of the third position is lower than the position height of the first position, the position height of the third position is H3, the position height of the first position is H1, and H1>H3.

[0134] Optionally, when the reagent needle starts to discharge the second liquid c, the needle tip of the reagent needle is in the second position, and the position height of the second position is H2, H2>H1>H3, and / or, when the reagent needle completes the second liquid c discharge operation, the needle tip of the reagent needle is in the second position, and the position height of the second position is H2, H2>H1, and the second liquid discharge position is higher than the first position, which can avoid contamination caused by a large area of ​​the outer surface of the needle being immersed in the liquid surface during the second liquid discharge process.

[0135] Optionally, when the reagent needle completes the discharge operation of the second liquid c, the needle tip height of the reagent needle is less than or equal to the liquid level height of the reaction container at that time, so as to avoid liquid splashing at the needle tip when discharging the second liquid.

[0136] In some embodiments, during the process of the pipetting needle performing the liquid discharging operation, the controller executes:

[0137] Controlling the moving component to drive the pipetting needle to move into the reaction container, and when the tip of the pipetting needle reaches the first position, controlling the power component to drive the pipetting needle to discharge the first liquid, the isolation gas and part of the second liquid in the needle body of the pipetting needle;

[0138] During the discharge of the second liquid, the moving component is controlled to drive the pipetting needle to rise, and during the lifting of the pipetting needle, the power component is controlled to drive the pipetting needle to discharge another portion of the second liquid into the reaction container.

[0139] See also Figure 7 When it is necessary to control the reagent needle to discharge liquid into the reaction container 4011, the second moving component is controlled to drive the reagent needle to move into the reaction container 4011, and when the needle tip of the reagent needle reaches the first position, at this time, the position height of the needle tip of the reagent needle is H1, and the controller 70 controls the second power component to drive the reagent needle to discharge the first liquid a, isolation gas b and part of the second liquid c in the needle body of the reagent needle.

[0140] After the reagent needle discharges part of the second liquid c, the controller 70 controls the second moving component to drive the reagent needle to rise, and controls the second power component to drive the reagent needle to discharge another part of the second liquid c into the reaction container 4011 during the reagent needle rising process.

[0141] When the second liquid c discharge operation is completed, the needle tip of the reagent needle is at the second position, and the position height of the second position is H2, H2>H1.

[0142] Optionally, when the reagent needle completes the discharge operation of the second liquid c, the needle tip height of the reagent needle is less than or equal to the liquid level height of the reaction container at that time.

[0143] In some embodiments, the sample analyzer is provided with different first and second liquid discharge modes;

[0144] When the sample analyzer is in the first liquid discharge mode, the controller is used to:

[0145] Controlling the driving mechanism to drive the pipetting needle to move into the reaction container, and controlling the driving mechanism to drive the pipetting needle to perform a liquid discharge operation, so as to discharge the first liquid, the isolation gas, and the second liquid in the pipetting needle into the reaction container;

[0146] Wherein, the first liquid is a concentrated reagent, and when the pipette needle discharges the isolation gas, the needle tip of the pipette needle is in a first position, and the height of the first position is lower than the liquid level of the liquid carried by the reaction container when the pipette needle discharges the isolation gas, or, the height of the first position is within a preset height range above the liquid level of the liquid carried by the reaction container when the pipette needle discharges the isolation gas.

[0147] Exemplarily, the sample analyzer is provided with different first discharge modes and second discharge modes, that is, the first discharge mode and the second discharge mode are adapted to different detection items, and different discharge modes are adapted based on different detection items, thereby making the sample analyzer more scenario-applicable.

[0148] In some embodiments, when the sample analyzer is in the second liquid discharge mode, the pipette needle is at least used to discharge the third liquid in the pipette needle into the reaction container, and the controller is further used to:

[0149] The driving mechanism is controlled to drive the pipetting needle to move into the reaction container, and when the needle tip of the pipetting needle is at the fourth position, the driving mechanism is controlled to drive the pipetting needle to discharge the third liquid into the reaction container.

[0150] Exemplarily, when the sample analyzer is in the second liquid discharge mode, the reagent needle is used to discharge the third liquid in the reagent needle into the reaction container while performing a liquid discharge operation into the reaction container. When the reagent needle is discharging the third liquid, the controller 70 controls the second driving mechanism to drive the reagent needle to move into the reaction container 4011, and when the needle tip of the reagent needle is in the fourth position, controls the second driving mechanism to drive the reagent needle to discharge the third liquid into the reaction container.

[0151] Optionally, when the reagent needle completes discharging the third liquid, the needle tip of the reagent needle is still in the fourth position, and when the third liquid is completed, the needle tip height of the reagent needle is less than or equal to the liquid level height of the liquid in the reaction container at this time.

[0152] Optionally, the third liquid is different from the first liquid and the second liquid. For example, the third liquid is a mixed solution of the first liquid and the second liquid.

[0153] The following will describe the liquid discharge control method provided in the embodiment of the present application in combination with the working principle of the sample analyzer 100 .

[0154] See also Figure 8 , an embodiment of the present application also provides a liquid discharge control method, which is applied to the aforementioned sample analyzer.

[0155] like Figure 8 As shown, the liquid discharge control method includes steps S101 to S102.

[0156] Step S101: controlling the driving mechanism of the sample analyzer to drive the pipette needle of the sample analyzer to move into a reaction container.

[0157] Step S102: controlling the driving mechanism to drive the pipette needle to perform a liquid discharge operation, wherein the liquid discharge operation at least includes discharging a first liquid, a second liquid, and an isolation gas between the first liquid and the second liquid in the pipette needle;

[0158] Wherein, when the pipette needle expels the isolation gas, the needle tip of the pipette needle is in a first position, and the height of the first position is less than or equal to the liquid level height of the liquid in the reaction container when the pipette needle expels the isolation gas, or, the height of the first position is within a preset height range above the liquid level of the liquid carried by the reaction container when the pipette needle expels the isolation gas.

[0159] In some embodiments, the driving mechanism includes a moving component for driving the pipetting needle to move, and a power component for providing the pipetting needle with a pipetting power; the driving mechanism controlling the sample analyzer drives the pipetting needle of the sample analyzer to move into a reaction container, and controls the driving mechanism to drive the pipetting needle to perform a liquid discharge operation, including:

[0160] Controlling the moving component to drive the pipetting needle to move into the reaction container, and controlling the power component to drive the pipetting needle to discharge the first liquid and the isolation gas into the reaction container;

[0161] During the process of discharging the isolation gas or after the isolation gas is discharged, the moving component is controlled to drive the pipetting needle to lift and the power component is controlled to drive the pipetting needle to discharge the second liquid into the reaction container.

[0162] It should be noted that those skilled in the art can clearly understand that, for the convenience and brevity of description, the specific working process of the above-described discharge control method can refer to the corresponding working process of the aforementioned sample analyzer and will not be repeated here.

[0163] It should be understood that the terms used in this application specification are only for the purpose of describing specific embodiments and are not intended to limit the application. As used in this application specification and the appended claims, unless the context clearly indicates otherwise, the singular forms "a", "an" and "the" are intended to include plural forms.

[0164] It should also be understood that the term "and / or" used in the specification of this application and the appended claims refers to any combination of one or more of the associated listed items and all possible combinations, including these combinations. It should be noted that, in this article, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or system including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or system. In the absence of further restrictions, an element defined by the sentence "including a..." does not exclude the presence of other identical elements in the process, method, article or system including the element.

[0165] The serial numbers of the embodiments of the present application are for description only and do not represent the advantages and disadvantages of the embodiments. The above is only a specific implementation of the present application, but the protection scope of the present application is not limited thereto. Any technician familiar with the technical field can easily think of various equivalent modifications or replacements within the technical scope disclosed in the present application, and these modifications or replacements should be included in the protection scope of the present application. Therefore, the protection scope of the present application shall be based on the protection scope of the claims.

Claims

1. A sample analyzer, characterized in that: include: A reaction device is provided with at least one reaction position, wherein the reaction position is used to place a reaction container; A pipette needle, at least used to perform a liquid discharge operation, wherein the liquid discharge operation at least includes discharging a first liquid, a second liquid, and an isolation gas between the first liquid and the second liquid in the pipette needle; A driving mechanism, at least used to drive the pipetting needle to perform the liquid discharging operation; and A controller is connected to the driving mechanism and is used to: Controlling the driving mechanism to drive the pipetting needle to move into the reaction container, and controlling the driving mechanism to drive the pipetting needle to perform a liquid discharge operation, so as to discharge the first liquid, the isolation gas, and the second liquid in the pipetting needle into the reaction container; Wherein, when the pipette needle expels the isolation gas, the needle tip of the pipette needle is in a first position, and the height of the first position is less than or equal to the liquid level of the liquid in the reaction container when the pipette needle expels the isolation gas, or, the height of the first position is within 2 mm above the liquid level of the liquid carried by the reaction container when the pipette needle expels the isolation gas.

2. The sample analyzer according to claim 1, characterized in that: When the pipetting needle completes discharging the second liquid into the reaction container, the needle tip height of the pipetting needle is less than or equal to the liquid level height of the liquid in the reaction container.

3. The sample analyzer according to claim 1, characterized in that: During the process in which the pipette needle performs the liquid discharging operation, the height of the discharge position of the second liquid is greater than the height of the discharge position of the isolation gas.

4. The sample analyzer according to claim 3, characterized in that: During the process of the pipetting needle performing the liquid discharging operation, the height of the discharge position of the isolation gas is greater than or equal to the height of the discharge position of the first liquid.

5. The sample analyzer according to claim 1, characterized in that: The height of the discharge position of the second liquid is determined according to the amount of the second liquid to be discharged by the pipette needle; and / or the volume of the isolation gas carried in the needle body of the pipette needle is determined according to the amount of the second liquid in the needle body of the pipette needle.

6. The sample analyzer according to claim 1, characterized in that: The first liquid includes a concentrated reagent, and the second liquid includes a diluent.

7. The sample analyzer according to claim 1, characterized in that: The sample analyzer further comprises a liquid supply device for providing at least a first liquid. Before controlling the driving mechanism to drive the pipette needle to move into the reaction container, the controller is further configured to: Controlling the driving mechanism to drive the pipetting needle to perform a liquid aspiration operation toward the liquid supply device, so as to aspirate the first liquid into the pipetting needle; Wherein, the controller is also used to control the driving mechanism to drive the pipette needle to inhale the isolation gas before performing the liquid aspiration operation.

8. The sample analyzer according to any one of claims 1 to 7, characterized in that: The driving mechanism includes a moving component for driving the pipetting needle to move, and a power component for providing the pipetting needle with a pipetting power; during the process of the pipetting needle performing the discharge operation, the controller is also used to: Controlling the power assembly to drive the pipette needle to discharge the first liquid and the isolation gas into the reaction container; During the process of discharging the isolation gas or after the isolation gas is discharged, the moving component is controlled to drive the pipetting needle to lift and the power component is controlled to drive the pipetting needle to discharge the second liquid into the reaction container.

9. The sample analyzer according to claim 8, characterized in that: During the process in which the pipette needle performs the liquid discharging operation, the controller is further used to: Controlling the moving assembly to drive the pipetting needle to move into the reaction container, and when the needle tip of the pipetting needle is at the first position, controlling the power assembly to drive the pipetting needle to discharge the first liquid and the isolation gas in the needle body of the pipetting needle; After the isolation gas is discharged, the moving component is controlled to drive the pipette needle to rise to a second position, and at the second position, the power component is controlled to drive the pipette needle to discharge the second liquid in the needle body of the pipette needle, and the position height of the second position is greater than the position height of the first position.

10. The sample analyzer according to claim 8, characterized in that: During the process in which the pipette needle performs the liquid discharging operation, the controller is further used to: Controlling the moving assembly to drive the pipetting needle to move into the reaction container, and when the needle tip of the pipetting needle is at the third position, controlling the power assembly to drive the pipetting needle to discharge the first liquid in the needle body of the pipetting needle; After completing the discharge of the first liquid, the moving component is controlled to drive the pipette needle to rise, and during the lifting process of the pipette needle, the power component is controlled to drive the pipette needle to discharge the isolation gas and the second liquid in the needle body of the pipette needle into the reaction container; wherein, when the pipette needle discharges the isolation gas, the needle tip of the pipette needle is in the first position, and the position height of the third position is lower than the position height of the first position.

11. The sample analyzer according to claim 8, characterized in that: During the process of the pipetting needle performing the liquid discharging operation, the controller executes: Controlling the moving assembly to drive the pipetting needle to move into the reaction container, and when the tip of the pipetting needle reaches the first position, controlling the power assembly to drive the pipetting needle to discharge the first liquid, the isolation gas, and part of the second liquid in the needle body of the pipetting needle; During the discharge of the second liquid, the moving component is controlled to drive the pipetting needle to rise, and during the lifting of the pipetting needle, the power component is controlled to drive the pipetting needle to discharge another portion of the second liquid into the reaction container.

12. A sample analyzer, characterized in that: The sample analyzer comprises: A reaction device is provided with at least one reaction position, wherein the reaction position is used to place a reaction container; A pipette needle, at least used to perform a liquid discharge operation, wherein the liquid discharge operation at least includes discharging a first liquid, a second liquid, and an isolation gas between the first liquid and the second liquid in the pipette needle; A driving mechanism, at least used to drive the pipetting needle to perform the liquid discharging operation, wherein the driving mechanism includes a moving component used to drive the pipetting needle to move, and a power component used to provide the pipetting needle with a liquid discharging power; and A controller is connected to the driving mechanism and is used to: Controlling the moving component to drive the pipetting needle to move into the reaction container, and controlling the power component to drive the pipetting needle to discharge the first liquid and the isolation gas into the reaction container; During the isolation gas discharge process or after the isolation gas discharge is completed, the moving component is controlled to drive the pipetting needle to lift and the power component is controlled to drive the pipetting needle to discharge the second liquid into the reaction container.

13. The sample analyzer according to claim 12, characterized in that: When the pipette needle discharges the isolation gas, the needle tip of the pipette needle is in a first position, and when the pipette needle discharges the second liquid, the needle tip of the pipette needle is in a second position, and the position height of the first position is smaller than the position height of the second position.

14. The sample analyzer according to claim 13, characterized in that: When the pipetting needle finishes discharging the second liquid into the reaction container, the needle tip height of the pipetting needle is less than or equal to the liquid level height of the liquid in the reaction container.

15. A sample analyzer, characterized in that: The sample analyzer comprises: A reaction device is provided with at least one reaction position, wherein the reaction position is used to place a reaction container; A pipette needle, at least used to perform a liquid discharge operation, wherein the liquid discharge operation at least includes discharging a first liquid, a second liquid, and an isolation gas between the first liquid and the second liquid in the pipette needle; A driving mechanism, at least used to drive the pipetting needle to perform the liquid discharging operation; and A controller connected to the driving mechanism; Wherein, the sample analyzer is provided with different first liquid discharge modes and second liquid discharge modes; When the sample analyzer is in the first liquid discharge mode, the controller is used to: Controlling the driving mechanism to drive the pipetting needle to move into the reaction container, and controlling the driving mechanism to drive the pipetting needle to perform a liquid discharge operation, so as to discharge the first liquid, the isolation gas, and the second liquid in the pipetting needle into the reaction container; Wherein, the first liquid is a concentrated reagent, and when the pipette needle discharges the isolation gas, the needle tip of the pipette needle is in a first position, and the height of the first position is lower than the liquid level of the liquid carried by the reaction container when the pipette needle discharges the isolation gas, or, the height of the first position is within 2 mm above the liquid level of the liquid carried by the reaction container when the pipette needle discharges the isolation gas.

16. The sample analyzer according to claim 15, characterized in that: When the sample analyzer is in the second liquid discharge mode, the pipette needle is at least used to discharge the third liquid in the pipette needle into the reaction container, and the controller is further used to: The driving mechanism is controlled to drive the pipetting needle to move into the reaction container, and when the needle tip of the pipetting needle is at the fourth position, the driving mechanism is controlled to drive the pipetting needle to discharge the third liquid into the reaction container.

17. A method for controlling liquid discharge, characterized in that: Applied to a sample analyzer, the method comprises: Controlling the driving mechanism of the sample analyzer to drive the pipette needle of the sample analyzer to move into the reaction container, and controlling the driving mechanism to drive the pipette needle to perform a liquid discharge operation, wherein the liquid discharge operation at least includes discharging a first liquid, a second liquid, and an isolation gas between the first liquid and the second liquid in the pipette needle; Wherein, when the pipette needle expels the isolation gas, the needle tip of the pipette needle is in a first position, and the height of the first position is less than or equal to the liquid level of the liquid in the reaction container when the pipette needle expels the isolation gas, or, the height of the first position is within 2 mm above the liquid level of the liquid carried by the reaction container when the pipette needle expels the isolation gas.

18. The method according to claim 17, characterized in that The driving mechanism includes a moving component for driving the pipetting needle to move, and a power component for providing the pipetting needle with a pipetting power; the driving mechanism for controlling the sample analyzer drives the pipetting needle of the sample analyzer to move into a reaction container, and controls the driving mechanism to drive the pipetting needle to perform a liquid discharge operation, including: Controlling the moving component to drive the pipetting needle to move into the reaction container, and controlling the power component to drive the pipetting needle to discharge the first liquid and the isolation gas into the reaction container; During the process of discharging the isolation gas or after the isolation gas is discharged, the moving component is controlled to drive the pipetting needle to lift and the power component is controlled to drive the pipetting needle to discharge the second liquid into the reaction container.

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