Nanometer seed treatment suspending agent as well as preparation method and application thereof

Abstract

The invention provides a nano seed treatment suspending agent as well as a preparation method and application thereof, and belongs to the technical field of crop pest control. The nano seed treatment suspending agent is prepared from the following raw materials in parts by weight: 0.1-10 parts of metalaxyl-M, 0.1-10 parts of fludioxonil, 0.1-30 parts of thiabendazole, 1-15 parts of a dispersing agent, 2-5 parts of a wetting agent, 3-12 parts of a film-forming agent, 6-14 parts of a warning agent, 5-7 parts of an anti-freezing agent, 3-5 parts of a thickening agent, 0.2-0.8 part of a de-foaming agent and the balance of water, totaling 100 parts. According to the nano seed treatment suspending agent, the seed treatment suspending agent with the particle size distribution of 200-500 nm is obtained through the wetting agent, the dispersing agent, the thickening agent and other raw materials, the smaller particle size enables active drugs to permeate seed epidermis to enter seeds, infection of pathogenic bacteria is reduced, and through compounding of metalaxyl-M, fludioxonil and thiabendazole, the seed treatment effect is improved. The control effect on crop diseases is improved, plant growth is promoted, and the total dosage is reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of crop disease and insect pest control, and in particular relates to a nano seed treatment suspension and a preparation method and application thereof. Background Art

[0002] As we all know, pests and diseases of crops such as wheat, barley, corn, soybeans, and peanuts during their growth period are the main threats to agricultural safety production. For example, in normal years, in order to reduce the losses caused by peanut pests and diseases such as root rot, 5 to 6 sprayings are often carried out, and in years with large outbreaks, the number of times the drug is used is as high as 8 to 10 times. The traditional stem and leaf spraying method for controlling pests and diseases of crops such as peanuts has many times of application and low utilization rate of the drug, which can easily cause drug resistance of peanut pests and diseases, and also cause great pollution to the environment, thus causing a series of other social problems. In addition, many times of application and low utilization rate of the drug not only affect environmental safety, but also increase the cost of planting crops. Therefore, how to reduce the amount of pesticides applied and the number of times the pesticides are used are problems that need to be solved in current agricultural production.

[0003] Treating seeds with seed treatment agents is one of the economical and simple methods to prevent and control underground pests and soil-borne and seed-borne diseases of crops. Compared with conventional spraying, dusting and soil treatment, seed treatment technology is the most economical and effective method for preventing and controlling crop diseases and insect pests. It can reduce the amount of pesticides used and the number of times pesticides are used during the crop growth period. Therefore, seed treatment technology has become an important choice for preventing and controlling crop diseases and insect pests due to its simple and easy operation method, effectiveness and economy.

[0004] However, since the seed coat of crop seeds such as peanut seeds is relatively loose and the particles are large, pathogens may be generated inside the seed coat. Ordinary seed treatment suspensions in the prior art cannot penetrate the seed coat well. If the coating dosage is too small, good prevention and control effects will not be achieved, and if the dosage is too large, pesticide damage will occur. Therefore, ordinary seed treatment agents are not ideal for preventing and controlling seed-borne diseases of crops, have low safety, and need to be supplemented with foliar sprays. Multiple applications of pesticides are required during the growth period of crops to effectively control seed-borne diseases of crops such as peanut root rot, which requires a lot of manpower and material resources. Summary of the invention

[0005] In view of this, the object of the present invention is to provide a nano seed treatment suspension and a preparation method and application thereof, wherein the nano seed treatment suspension can improve the control effect of seed-borne diseases of crops.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The invention provides a nano seed treatment suspension, which is composed of the following raw materials in parts by weight: 0.1-10 parts of metalaxyl-M, 0.1-10 parts of fludioxonil, 0.1-30 parts of thiabendazole, 1-15 parts of a dispersant, 2-5 parts of a wetting agent, 3-12 parts of a film-forming agent, 6-14 parts of a warning agent, 5-7 parts of an antifreeze agent, 3-5 parts of a thickener, 0.2-0.8 parts of a defoaming agent, and water supplemented to 100 parts;

[0008] The dispersant is a hyperbranched polymer dispersant and / or a phosphate;

[0009] The thickener is magnesium aluminum silicate and cellulose, and the mass ratio of magnesium aluminum silicate to cellulose is 0.5 to 1.5:3;

[0010] The wetting agent is acetylenic glycol polyoxyethylene ether.

[0011] Preferably, the nano seed treatment suspension is composed of the following raw materials in parts by weight: 1-7 parts of metalaxyl-M, 0.5-3 parts of fludioxonil, 5-20 parts of thiabendazole, 3-10 parts of dispersant, 2-4 parts of wetting agent, 3-12 parts of film-forming agent, 6-14 parts of warning agent, 5-7 parts of antifreeze agent, 3-5 parts of thickener, 0.2-0.8 parts of defoaming agent, and water is added to 100 parts.

[0012] Preferably, the hyperbranched polymer dispersant is hyperbranched polymer dispersant F031, the phosphate is phosphate FS-3000, when the dispersant is a hyperbranched polymer dispersant and a phosphate, the mass ratio of the hyperbranched polymer dispersant to the phosphate is 2-4:3-4; the acetylene glycol polyoxyethylene ether is acetylene glycol polyoxyethylene ether MCQ-803; the cellulose is cellulose MC-S171.

[0013] Preferably, the film-forming agent includes polyacrylic acid resins; the warning agent includes at least one of acid scarlet and alkaline rose essence; the antifreeze agent includes at least one of glycerol and ethylene glycol; and the defoaming agent includes AF defoaming agent.

[0014] Preferably, the polyacrylic acid resin is polyacrylic acid resin 0062.

[0015] Preferably, the particle size of the nano seed treatment suspending agent is 200 to 500 nm.

[0016] The present invention provides a method for preparing the above-mentioned nano seed treatment suspension, comprising the following steps:

[0017] The metalaxyl-M, fludioxonil, thiabendazole, dispersant, wetting agent, film-forming agent, warning agent, antifreeze agent, thickener, defoaming agent and water are mixed evenly and then sand-milled to obtain a nano seed treatment suspension.

[0018] Preferably, the sand is ground to a particle size of 200 to 500 nm.

[0019] The present invention also provides an application of the nano seed treatment suspension or the preparation method in promoting plant growth, wherein the plant growth traits include one or more of plant seedling uniformity, absence of deformed seedlings, plant height and plant germination rate.

[0020] The present invention also provides an application of the nano seed treatment suspension or the preparation method in preventing and treating plant root rot.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] The invention provides a nano seed treatment suspension, a preparation method and an application thereof. The nano seed treatment suspension of the invention is a seed treatment suspension with a particle size distribution of 200 to 500 nm obtained by using raw materials such as a wetting agent, a dispersant and a thickener. The smaller particle size allows active drugs to penetrate the seed epidermis and enter the seeds, thereby reducing the infection of pathogenic bacteria. Meanwhile, by compounding three active ingredients of metalaxyl-M, fludioxonil and thiabendazole, the control effect on crop diseases is improved, plant growth is promoted and the total amount of drugs used is reduced. DETAILED DESCRIPTION

[0023] The present invention aims to solve the technical problems that the seed treatment agents in the prior art cannot penetrate the seed coat of crop seeds such as peanut seeds well, and cannot achieve high prevention effect at a low dosage with higher safety, resulting in unsatisfactory prevention and control effect on crop seed-borne diseases and low safety.

[0024] The invention provides a nano seed treatment suspension, which is composed of the following raw materials in parts by weight: 0.1-10 parts of metalaxyl-M, 0.1-10 parts of fludioxonil, 0.1-30 parts of thiabendazole, 1-15 parts of a dispersant, 2-5 parts of a wetting agent, 3-12 parts of a film-forming agent, 6-14 parts of a warning agent, 5-7 parts of an antifreeze agent, 3-5 parts of a thickener, 0.2-0.8 parts of a defoaming agent, and water supplemented to 100 parts;

[0025] The dispersant is a hyperbranched polymer dispersant and / or a phosphate;

[0026] The thickener is magnesium aluminum silicate and cellulose, and the mass ratio of magnesium aluminum silicate to cellulose is 0.5 to 1.5:3;

[0027] The wetting agent is acetylenic glycol polyoxyethylene ether.

[0028] In the present invention, metalaxyl-M is also called high-efficiency metalaxyl, and its chemical name is N-(2,6-xylyl)-N-(methoxyacetyl)-D-alanine methyl ester. Metalaxyl-M is a light brown, viscous, transparent liquid. The solubility of metalaxyl-M in water is 26g / L (25°C, pure product). Fludioxonil is also called fludioxonil and siloxane, and its chemical name is 4-(2,2-difluoro-1,3-benzodioxy-4-yl)pyrrole-3-carbonitrile. Prochloraz belongs to the imidazole fungicide, and its mechanism of action is to inhibit the formation of microtubules during fungal mitosis. Prochloraz has obvious preventive effect on diseases caused by ascomycetes, basidiomycetes and ascomycetes. The nano seed treatment suspension of the present invention improves the prevention and control effect of crop diseases by compounding the three active ingredients of metalaxyl-M, fludioxonil and prochloraz, while reducing the total amount of medicine used. In addition, the active ingredients are nano-scale particles that can effectively adhere to the surface of seeds and can effectively penetrate the seed coat and enter the interior of the seeds, further improving the control effect of the nano seed treatment suspension of the present application on crop diseases, especially improving the control effect on seed-borne diseases such as peanut root rot.

[0029] As one embodiment, the nano seed treatment suspension is preferably composed of the following raw materials in parts by weight: 1 to 7 parts of metalaxyl-M, 0.5 to 3 parts of fludioxonil, 5 to 20 parts of thiabendazole, 3 to 10 parts of dispersant, 2 to 4 parts of wetting agent, 3 to 12 parts of film-forming agent, 6 to 14 parts of warning agent, 5 to 7 parts of antifreeze agent, 3 to 5 parts of thickener, 0.2 to 0.8 parts of defoaming agent, and water is added to 100 parts.

[0030] The present invention can further promote the synergistic effect between the active ingredients by placing metalaxyl-M, fludioxonil and thiabendazole within the above-mentioned weight range, enhance the preventive and control effect of the nano seed treatment suspension of the present invention on crop seed-borne diseases such as peanut root rot, improve the preventive effect, and is conducive to further reducing the total amount of metalaxyl-M, fludioxonil and thiabendazole, thereby reducing the use of pesticides.

[0031] In the present invention, the hyperbranched polymer dispersant is a hyperbranched polymer dispersant F031, and the phosphate is a phosphate FS-3000. When the dispersant is a hyperbranched polymer dispersant and a phosphate, the mass ratio of the hyperbranched polymer dispersant and the phosphate is 2 to 4:3 to 4, and more preferably 3:3, 2:3 or 4:4. The dispersant has good dispersibility for metalaxyl-M, fludioxonil and thiabendazole, and the dispersed phase formed after dispersion has good stability, so that the nano seed treatment suspension of the present invention is stable, and the content of the active ingredient is stable and storage-resistant. The mass ratio of the hyperbranched polymer dispersant and the phosphate is controlled within the range of 2 to 4:3 to 4, so that the three active ingredients of the nano seed treatment suspension have good stability. In addition, since the dispersant has good dispersibility for active ingredients, the concentration of active ingredients can be made higher. When the same dispersed phase infiltrates the seed coat, it can carry more active ingredients into the seed, improve the utilization rate of the active ingredients, and provide the prevention effect of the nano seed treatment suspension on crop diseases. In the present invention, the hyperbranched polymer dispersant is purchased from Shanghai Maicai, with a trade name of F031, so the present invention will purchase from Shanghai Maicai, with a trade name of F031, named hyperbranched polymer dispersant F031; phosphates are purchased from Jieshi Chemical, with a trade name of FS-3000, so the present invention will purchase from Jieshi Chemical, with a trade name of FS-3000, and the phosphates are named phosphate FS-3000.

[0032] In the present invention, the film-forming agent includes polyacrylic acid resins, and the polyacrylic acid resins are polyacrylic acid resin 0062, which is purchased from Shanghai Keying under the trade name 0062. The present invention names the polyacrylic acid resin purchased from Shanghai Keying under the trade name 0062 as polyacrylic acid resin 0062; the film-forming agent has good film-forming property, and the formed film is uniform, stable, and not easy to fall off, and can effectively prevent the loss of active ingredients on the surface of seeds and inside seeds. The weight content of the film-forming agent can be specifically 5 parts, 7 parts, 8.5 parts, 9 parts or 10 parts. The content of the film-forming agent is controlled within the above range, so that when the nano seed treatment suspension is used to treat the seeds, a thin film can be formed on the surface of the seeds, and the film formation is fast, the film formation rate is high, the shedding rate is low, the film is uniform and stable, and the active ingredients and seeds can be effectively coated, so that the active ingredients can fully act on the seeds.

[0033] In the present invention, the antifreeze agent includes at least one of propylene glycol and ethylene glycol, and the weight proportion of the antifreeze agent can be 5 parts, 6 parts or 7 parts. The mass of the antifreeze agent is controlled within this range, so that the prepared nano seed treatment suspension has excellent stability even at a low temperature of -18°C.

[0034] In the present invention, the weight portion of the defoamer includes 0.3 to 0.5 parts, and further 0.3 to 0.4 parts. The defoamer includes AF defoamer, which is purchased from Huntsman, and the AF defoamer is also called silicone defoamer AF. The quality of the defoamer is controlled within this range, so that the prepared nano seed treatment suspension has qualified persistent foaming properties, and the amount of foam during the sand milling process is also very small, which greatly improves the sand milling efficiency.

[0035] In the present invention, the weight portion of the wetting agent is preferably 2 to 4 parts, specifically, it can be 2 parts, 3 parts or 4 parts. Controlling the content of the wetting agent within this range can improve the stability and uniformity of the nano seed treatment suspension and reduce the particle size of the active ingredient particles. The wetting agent is preferably acetylene glycol polyoxyethylene ether, such as acetylene glycol polyoxyethylene ether MCQ-803, purchased from Shanghai Maicai, trade name MCQ-803, so the acetylene glycol polyoxyethylene ether purchased from Shanghai Maicai, trade name MCQ-803 is named acetylene glycol polyoxyethylene ether MCQ-803; the wetting effect of the wetting agent is good, so that the nano seed treatment suspension of the present invention has good stability and small particle size of the active ingredient particles.

[0036] In the present invention, the thickener is magnesium aluminum silicate and cellulose, the mass ratio of magnesium aluminum silicate to cellulose is 0.5-1.5:3, more preferably 1:3, the magnesium aluminum silicate is purchased from Suzhou Guojian Huitou, trade name SF-04; the cellulose is purchased from Shanghai Maicai, trade name MC-S171, and the cellulose purchased from Shanghai Maicai, trade name MC-S171, is named cellulose MC-S171. The thickener of the present invention has a good thickening effect, can ensure the good flow of the nano seed treatment suspension of the present invention, and has good physical stability.

[0037] In the present invention, the warning agent is 6 to 14 parts, specifically, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts or 13 parts. The warning agent includes at least one of acid scarlet and alkaline rose essence. The warning agent has a bright color, and its addition can play a good warning role.

[0038] In the present invention, the particle size of the nano seed treatment suspending agent is preferably 200 to 500 nm.

[0039] The present invention provides a method for preparing the above-mentioned nano seed treatment suspension, comprising the following steps:

[0040] The metalaxyl-M, fludioxonil, thiabendazole, dispersant, wetting agent, film-forming agent, warning agent, antifreeze agent, thickener, defoaming agent and water are mixed evenly and then sand-milled to obtain a nano seed treatment suspension.

[0041] In the present invention, the sand milling is performed to a particle size of 200 to 500 nm. The present invention uses the sand milling effect to form tiny nanoparticles of active ingredients that are stably dispersed in the aqueous phase, so that the active ingredients can penetrate the seed coat and enter the interior of the seed, thereby improving the control effect of the active ingredients on crop seed-borne diseases. The above-mentioned preparation method of the present invention has easy-to-control conditions and good reproducibility, and the prepared nano seed treatment suspension is stable and uniform. By compounding the three active ingredients of metalaxyl, fludioxonil and thiabendazole, the control effect on crop seed-borne diseases is effectively improved, while the total amount of medication is reduced.

[0042] In the present invention, the particle size of the nanoparticles in the nano seed treatment suspension is less than or equal to 500nm, further, it can be 200-500nm, specifically, it can be 200nm, or less than or equal to 210nm, 300nm, 400nm or 500nm. The present invention sets the particle size of the active ingredient particles within this range, which can improve the penetration ability of the active ingredient particles in the nano seed treatment suspension to the seed coat, so as to improve the utilization rate of the active ingredient in the nano seed treatment suspension, and improve the control effect of the nano seed treatment suspension on crop seed-borne diseases.

[0043] It should be noted that the particle size of the oil phase particles is less than or equal to a certain numerical value, which does not mean that the particle size of all active ingredient particles in the nano seed treatment suspension is less than or equal to the numerical value, but means that the particle size of a certain number (such as 90%) of the active ingredient particles in the nano seed treatment suspension is less than or equal to the numerical value. For example, in a specific example, the particle size of the active ingredient particles in the nano seed treatment suspension is less than or equal to 500nm, which means that the particle size distribution of the oil phase particles in the nano seed treatment suspension is D 90 Less than or equal to 500nm.

[0044] The present invention also provides an application of the nano seed treatment suspension or the preparation method in promoting plant growth, wherein the plant growth trait comprises one of plant seedling uniformity, absence of deformed seedlings, plant height and plant germination rate.

[0045] The present invention also provides an application of the nano seed treatment suspension or the preparation method in preventing and treating plant root rot.

[0046] Peanut root rot is one of the most serious diseases and pests of peanuts. It is a seed-borne disease of peanuts caused by fungi. Ordinary seed treatment agents, such as ordinary suspension concentrates and emulsions, are not ideal for the prevention and treatment of peanut root rot, and their safety is relatively low. They need to be supplemented by liquid surface spray and applied multiple times during the peanut growth cycle to effectively control the damage of peanut root rot.

[0047] The nano seed treatment suspension of the present invention or the nano seed treatment suspension prepared by the above preparation method, through the compounding of three active ingredients of metalaxyl-M, fludioxonil and thiabendazole, makes the active ingredients play a synergistic role, thereby improving the prevention effect of the nano seed treatment suspension on peanut root rot. The nano-scale active ingredients can effectively adhere to the surface of the peanut seeds and penetrate the peanut seed coat into the interior of the peanut seeds, thereby improving the utilization rate of the active ingredients, further improving the bactericidal activity of the nano seed treatment suspension on the pathogen of peanut root rot, and improving the prevention effect of the nano seed treatment suspension on peanut root rot.

[0048] In some embodiments, the nano seed treatment suspension of the embodiment of the present application or the nano seed treatment suspension prepared by the preparation method of the embodiment of the present application is used to prevent and treat peanut root rot, and the dosage of the nano seed treatment suspension is less than or equal to 150g / 100kg seeds, and further, it can be 100g / 100kg seeds to 150g / 100kg seeds, and specifically, it can be 100g / 100kg seeds, 120g / 100kg seeds, 150g / 100kg seeds, etc. Typical but non-limiting dosages. Controlling the dosage of the nano seed treatment suspension in the prevention and treatment of peanut root rot within this range can improve the safety of the nano seed treatment suspension to peanuts while improving the prevention effect, and improve the germination rate of peanut seeds and the overall quality of peanut seedlings.

[0049] In the present invention, unless otherwise specified, all raw material components are commercially available products well known to those skilled in the art.

[0050] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0051] In the following embodiments, hyperbranched polymer dispersants (purchased from Shanghai Maicai; trade name F031), non-ionic polymer types (purchased from Indorama; trade name 2500), phosphates (purchased from Jieshi Chemical; trade name FS-3000), block polyethers (purchased from Indorama; trade name 5500), phosphates (purchased from Xingfei Chemical; trade name SK-33SC) and carboxylates (purchased from Wuxi Yijingfeng; trade name 7023A).

[0052] Alkylnaphthalene sulfonates (purchased from Aksu; trade name EFW), acetylene glycol polyoxyethylene ethers (purchased from Shanghai Maicai; trade name MCQ-803), polyacrylic acid resins (purchased from Shanghai Keying; trade name 0062), polyacrylic acid resins (purchased from Tianjin Dekema; ​​trade name KMM20), magnesium aluminum silicate (purchased from Suzhou Guojian Huitou; trade name SF-04) and cellulose (purchased from Shanghai Maicai; trade name MC-S171).

[0053] Example 1

[0054] A nano seed treatment suspension is composed of the following raw materials in weight content: 1.8g of metalaxyl-M, 2.3g of fludioxonil, 13.9g of thiabendazole, 3g of hyperbranched polymer dispersant (F031), 3g of phosphates (FS-3000), 2g of acetylene glycol polyoxyethylene ethers (MCQ-803), 5g of polyacrylic acid resins (0062), 10g of acid scarlet, 6g of glycerol, 1g of magnesium aluminum silicate (SF-04), 3g of cellulose (MC-S171), 0.4g of AF defoamer, and water is added to make up to 100g.

[0055] The preparation method of the nano seed treatment suspension concentrate comprises the following specific steps:

[0056] The above-mentioned weights of metalaxyl-M, fludioxonil, thiabendazole, hyperbranched polymer dispersant (F031), phosphates (FS-3000), acetylene glycol polyoxyethylene ethers (MCQ-803), polyacrylic acid resins (0062), acid scarlet, glycerol, magnesium aluminum silicate (SF-04), cellulose (MC-S171), AF defoamer and water were weighed, mixed evenly, and then sand-milled in a sand mill to 200 nm to obtain a nano seed treatment suspension.

[0057] Example 2

[0058] A nano seed treatment suspending agent is composed of the following raw materials in weight content: 3.3g of metalaxyl-M, 1.1g of fludioxonil, 6.6g of thiabendazole, 2g of a hyperbranched polymer dispersant (F031), 3g of phosphates (FS-3000), 2g of acetylene glycol polyoxyethylene ethers (MCQ-803), 10g of polyacrylic acid resins (0062), 12g of acid scarlet, 6g of glycerol, 1g of magnesium aluminum silicate (SF-04), 3g of cellulose (MC-S171), 0.3g of an AF defoaming agent, and water is added to make up to 100g.

[0059] The preparation method of the nano seed treatment suspension concentrate comprises the following specific steps:

[0060] The above-mentioned weights of metalaxyl-M, fludioxonil, thiabendazole, hyperbranched polymer dispersant (F031), phosphates (FS-3000), acetylene glycol polyoxyethylene ethers (MCQ-803), polyacrylic acid resins (0062), acid scarlet, glycerol, magnesium aluminum silicate (SF-04), cellulose (MC-S171), AF defoamer and water were weighed, mixed evenly, and then sand-milled in a sand mill to 200 nm to obtain a nano seed treatment suspension.

[0061] Example 3

[0062] A nano seed treatment suspending agent is composed of the following raw materials in weight content: 5g of metalaxyl-M, 2g of fludioxonil, 18g of thiabendazole, 4g of a hyperbranched polymer dispersant (F031), 4g of phosphates (FS-3000), 2g of acetylene glycol polyoxyethylene ethers (MCQ-803), 8g of polyacrylic acid resins (0062), 8g of acid scarlet, 6g of glycerol, 1g of magnesium aluminum silicate (SF-04), 3g of cellulose (MC-S171), 0.6g of an AF defoaming agent, and water is added to make up to 100g.

[0063] The preparation method of the nano seed treatment suspension concentrate comprises the following specific steps:

[0064] The above-mentioned weights of metalaxyl-M, fludioxonil, thiabendazole, hyperbranched polymer dispersant (F031), phosphates (FS-3000), acetylene glycol polyoxyethylene ethers (MCQ-803), polyacrylic acid resins (0062), acid scarlet, glycerol, magnesium aluminum silicate (SF-04), cellulose (MC-S171), AF defoamer and water were weighed, mixed evenly, and then sand-milled in a sand mill to 200 nm to obtain a nano seed treatment suspension.

[0065] Example 4

[0066] The difference between this embodiment and embodiment 1 is that 6 g of phosphate (FS-3000) is used in this embodiment to replace 3 g of hyperbranched polymer dispersant (F031) and 3 g of phosphate (FS-3000) in embodiment 1, and the remaining raw materials and their weight contents are the same as those in embodiment 1.

[0067] The steps of the method for preparing the nano seed treatment suspension in this embodiment are as follows:

[0068] The above-mentioned weights of metalaxyl-M, fludioxonil, thiabendazole, phosphates (FS-3000), acetylene glycol polyoxyethylene ethers (MCQ-803), polyacrylic acid resins (0062), acid red, glycerol, magnesium aluminum silicate (SF-04), cellulose (MC-S171), AF defoamer and water were weighed, mixed evenly, and then sand-milled in a sand mill to 200 nm to obtain a nano seed treatment suspension.

[0069] Example 5

[0070] The difference between this embodiment and embodiment 1 is that 6 g of hyperbranched polymer dispersant (F031) is used in this embodiment to replace 3 g of hyperbranched polymer dispersant (F031) and 3 g of phosphate (FS-3000) in embodiment 1, and the remaining raw materials and their weight contents are the same as those in embodiment 1.

[0071] The steps of the method for preparing the nano seed treatment suspension in this embodiment are as follows:

[0072] Weigh the above-mentioned weights of metalaxyl-M, fludioxonil, thiabendazole, hyperbranched polymer dispersant (F031), acetylene glycol polyoxyethylene ether (MCQ-803), polyacrylic acid resin (0062), acid red, glycerol, magnesium aluminum silicate (SF-04), cellulose (MC-S171), AF defoamer and water, mix them evenly, and then sand-mill them in a sand mill until the particle size is 200 m to obtain a nano seed treatment suspension.

[0073] Comparative Example 1

[0074] The difference between this comparative example and Example 1 is that in this comparative example, 6 g of non-ionic polymer type (2500) is used to replace 3 g of hyperbranched polymer dispersant (F031) and 3 g of phosphate (FS-3000) in Example 1, and the remaining raw materials and their weight contents are the same as those in Example 1.

[0075] The steps of the preparation method of the nano seed treatment suspension of this comparative example are as follows:

[0076] Weigh the above-mentioned weights of metalaxyl-M, fludioxonil, thiabendazole, non-ionic polymer type (2500), acetylene glycol polyoxyethylene ether (MCQ-803), polyacrylic resin (0062), acid red, glycerol, magnesium aluminum silicate (SF-04), cellulose (MC-S171), AF defoamer and water, mix them evenly, and then sand grind them in a sand mill to 200 nm to obtain a nano seed treatment suspension.

[0077] Comparative Example 2

[0078] The difference between this comparative example and Example 1 is that in this comparative example, 6 g of phosphate ester (SK-33SC) is used to replace 3 g of hyperbranched polymer dispersant (F031) and 3 g of phosphate (FS-3000) in Example 1, and the remaining raw materials and their weight contents are the same as those in Example 1.

[0079] The steps of the preparation method of the nano seed treatment suspension of this comparative example are as follows:

[0080] The above-mentioned weights of metalaxyl-M, fludioxonil, thiabendazole, phosphates (SK-33SC), acetylene glycol polyoxyethylene ethers (MCQ-803), polyacrylic acid resins (0062), acid red, glycerol, magnesium aluminum silicate (SF-04), cellulose (MC-S171), AF defoamer and water were weighed, mixed evenly, and then sand-milled in a sand mill to 200 nm to obtain a nano seed treatment suspension.

[0081] Comparative Example 3

[0082] The difference between this comparative example and Example 1 is that in this comparative example, 6 g of carboxylate (7023A) is used to replace 3 g of hyperbranched polymer dispersant (F031) and 3 g of phosphate (FS-3000) in Example 1, and the remaining raw materials and their weight contents are the same as those in Example 1.

[0083] The steps of the preparation method of the nano seed treatment suspension of this comparative example are as follows:

[0084] The above-mentioned weights of metalaxyl-M, fludioxonil, thiabendazole, carboxylates (7023A), acetylene glycol polyoxyethylene ethers (MCQ-803), polyacrylic acid resins (0062), acid scarlet, glycerol, magnesium aluminum silicate (SF-04), cellulose (MC-S171), AF defoamer and water were weighed, mixed evenly, and then sand-milled in a sand mill to 200 nm to obtain a nano seed treatment suspension.

[0085] Comparative Example 4

[0086] The difference between this comparative example and Example 1 is that in this comparative example, 6 g of block polyether (5500) is used to replace 3 g of hyperbranched polymer dispersant (F031) and 3 g of phosphate (FS-3000) in Example 1, and the remaining raw materials and their weight contents are the same as those in Example 1.

[0087] The steps of the preparation method of the nano seed treatment suspension of this comparative example are as follows:

[0088] The above-mentioned weights of metalaxyl-M, fludioxonil, thiabendazole, block polyethers (5500), acetylene glycol polyoxyethylene ethers (MCQ-803), polyacrylic acid resins (0062), acid red, glycerol, magnesium aluminum silicate (SF-04), cellulose (MC-S171), AF defoamer and water were weighed, mixed evenly, and then sand-milled in a sand mill to 200 nm to obtain a nano seed treatment suspension.

[0089] Test Example 1

[0090] The nano seed treatment suspension prepared in Examples 4-5 and Comparative Examples 1-4 was divided into two equal parts, one of which was stored at 0±2°C for 14 days (cold storage), and the other was stored at 54±2°C for 14 days (hot storage). After being taken out, it was left to stand at room temperature for 3 hours to observe whether the particle size had grown and the dispersibility of the effective ingredients.

[0091] Table 1 Results of cold storage and hot storage stability of different groups

[0092]

[0093]

[0094] The results in Table 1 show that the cold storage and hot storage stability of the nano seed treatment suspension prepared by using F031 or FS-3000 are high, which is conducive to improving the stability of the nano seed treatment suspension. Therefore, a mixed solution of a hyperbranched polymer dispersant and a phosphate ester can be used as a dispersant for the nano seed treatment suspension, so that the metalaxyl-M, fludioxonil and thiabendazole technical drugs are fully dispersed in the aqueous phase, and the metalaxyl-M, fludioxonil and thiabendazole technical drugs are dispersed. The suspension has good stability.

[0095] Comparative Example 5

[0096] The difference between this comparative example and Example 1 is that in this comparative example, 2 g of alkyl naphthalene sulfonate (EFW) is used to replace 2 g of acetylene glycol polyoxyethylene ether (MCQ-803) in Example 1, and the remaining raw materials and their weight contents are the same as those in Example 1.

[0097] The steps of the preparation method of the nano seed treatment suspension of this comparative example are as follows:

[0098] The above-mentioned weights of metalaxyl-M, fludioxonil, thiabendazole, hyperbranched polymer dispersant (F031), phosphates (FS-3000), alkylnaphthalene sulfonates (EFW), polyacrylic acid resins (0062), acid red, glycerol, magnesium aluminum silicate (SF-04), cellulose (MC-S171), AF defoamer and water were weighed, mixed evenly, and then sand-milled in a sand mill to 200 nm to obtain a nano seed treatment suspension.

[0099] Test Example 2

[0100] Take the nano seed treatment suspension of Example 1 and Comparative Example 5, observe the properties of each portion of nano seed treatment suspension, and divide each portion of nano seed treatment suspension into 2 equal parts, and conduct cold storage stability and hot storage stability investigations respectively. The 2 equal parts of nano seed treatment suspension were placed at 0±2°C for 4d (cold storage) and 54±2°C for 14d (hot storage), and then taken out and left to stand at room temperature for 3h to observe whether the particle size grows, the dispersibility of the effective ingredients, and the sand milling efficiency (set to 1 to 5 levels, 5 levels are the highest, wherein 5 levels are sand milled by ordinary vertical sand mill for 1.5 hours, 4 levels are sand milled by ordinary vertical sand mill for 3 hours, 3 levels are sand milled by ordinary vertical sand mill for 4.5 hours, 2 levels are sand milled by ordinary vertical sand mill for 6 hours, and 1 level is sand milled by ordinary vertical sand mill for 7.5 hours), and the results are shown in Table 2.

[0101] Table 2 Results of the stability and sand milling efficiency of nanoseed treated suspensions with different types of wetting agents

[0102]

[0103] The results in Table 2 show that, compared with Comparative Example 5, Example 1 uses MCQ-803 as a wetting agent for the nano seed treatment suspension, which can improve the stability and uniformity of the nano seed treatment suspension of the present application example and reduce the particle size of the active ingredient particles.

[0104] Example 6

[0105] The difference between this embodiment and embodiment 1 is that the weight content of acetylene glycol polyoxyethylene ether (MCQ-803) in this embodiment is 3 g, and the remaining raw materials and their weight contents are the same as those in embodiment 1.

[0106] The preparation method of the nano seed treatment suspension concentrate comprises the following specific steps:

[0107] The above-mentioned weights of metalaxyl-M, fludioxonil, thiabendazole, hyperbranched polymer dispersant (F031), phosphates (FS-3000), acetylene glycol polyoxyethylene ethers (MCQ-803), polyacrylic acid resins (0062), acid scarlet, glycerol, magnesium aluminum silicate (SF-04), cellulose (MC-S171), AF defoamer and water were weighed, mixed evenly, and then sand-milled in a sand mill to 200 nm to obtain a nano seed treatment suspension.

[0108] Example 7

[0109] The difference between this embodiment and embodiment 1 is that the weight content of acetylene glycol polyoxyethylene ether (MCQ-803) in this embodiment is 4 g, and the remaining raw materials and their weight contents are the same as those in embodiment 1.

[0110] The preparation method of the nano seed treatment suspension concentrate comprises the following specific steps:

[0111] The above-mentioned weights of metalaxyl-M, fludioxonil, thiabendazole, hyperbranched polymer dispersant (F031), phosphates (FS-3000), acetylene glycol polyoxyethylene ethers (MCQ-803), polyacrylic acid resins (0062), acid scarlet, glycerol, magnesium aluminum silicate (SF-04), cellulose (MC-S171), AF defoamer and water were weighed, mixed evenly, and then sand-milled in a sand mill to 200 nm to obtain a nano seed treatment suspension.

[0112] Comparative Example 6

[0113] The difference between this comparative example and Example 1 is that the weight content of acetylene glycol polyoxyethylene ether (MCQ-803) in this comparative example is 0.5 g, and the remaining raw materials and their weight contents are the same as those in Example 1.

[0114] The preparation method of the nano seed treatment suspension concentrate comprises the following specific steps:

[0115] The above-mentioned weights of metalaxyl-M, fludioxonil, thiabendazole, hyperbranched polymer dispersant (F031), phosphates (FS-3000), acetylene glycol polyoxyethylene ethers (MCQ-803), polyacrylic acid resins (0062), acid scarlet, glycerol, magnesium aluminum silicate (SF-04), cellulose (MC-S171), AF defoamer and water were weighed, mixed evenly, and then sand-milled in a sand mill to 200 nm to obtain a nano seed treatment suspension.

[0116] Comparative Example 7

[0117] The difference between this comparative example and Example 1 is that the weight content of the acetylene glycol polyoxyethylene ether (MCQ-803) in this comparative example is 1 g, and the remaining raw materials and their weight contents are the same as those in Example 1.

[0118] The preparation method of the nano seed treatment suspension concentrate comprises the following specific steps:

[0119] The above-mentioned weights of metalaxyl-M, fludioxonil, thiabendazole, hyperbranched polymer dispersant (F031), phosphates (FS-3000), acetylene glycol polyoxyethylene ethers (MCQ-803), polyacrylic acid resins (0062), acid scarlet, glycerol, magnesium aluminum silicate (SF-04), cellulose (MC-S171), AF defoamer and water were weighed, mixed evenly, and then sand-milled in a sand mill to 200 nm to obtain a nano seed treatment suspension.

[0120] Comparative Example 8

[0121] The difference between this comparative example and Example 1 is that the weight content of acetylene glycol polyoxyethylene ether (MCQ-803) in this comparative example is 1.5 g, and the other raw materials and their weight contents are the same as those in Example 1.

[0122] The preparation method of the nano seed treatment suspension concentrate comprises the following specific steps:

[0123] The above-mentioned weights of metalaxyl-M, fludioxonil, thiabendazole, hyperbranched polymer dispersant (F031), phosphates (FS-3000), acetylene glycol polyoxyethylene ethers (MCQ-803), polyacrylic acid resins (0062), acid scarlet, glycerol, magnesium aluminum silicate (SF-04), cellulose (MC-S171), AF defoamer and water were weighed, mixed evenly, and then sand-milled in a sand mill to 200 nm to obtain a nano seed treatment suspension.

[0124] Test Example 3

[0125] Take the nano seed treatment suspension of Comparative Examples 6 to 8, Example 1, Example 6 and Example 7, observe the properties and sand milling efficiency of each nano seed treatment suspension, and divide each nano seed treatment suspension into 2 equal parts. The 2 equal parts of nano seed treatment suspension were stored at 0±2°C for 14d (cold storage) and 54±2°C for 14d (hot storage), respectively, and left to stand at room temperature for 3h after being taken out, and observe whether the particle size grows and the sand milling efficiency (set to 1 to 5 levels, 5 levels are the highest, wherein 5 levels are sand milled for 1.5 hours by ordinary vertical sand mill, 4 levels are sand milled for 3 hours by ordinary vertical sand mill, 3 levels are sand milled for 4.5 hours by ordinary vertical sand mill, 2 levels are sand milled for 6 hours by ordinary vertical sand mill, and 1 level is sand milled for 7.5 hours by ordinary vertical sand mill), and the results are shown in Table 3.

[0126] Table 3 Stability and sand milling efficiency results of nano seed treated suspension with different amounts of wetting agent

[0127] Group Wetting agent dosage (g) Sanding efficiency Thermal storage stability Cold storage stability Comparative Example 6 0.5 Level 2 Particle size growth Stablize Comparative Example 7 1 Level 3 Relatively stable Stablize Comparative Example 8 1.5 Level 4 Stablize Stablize Example 1 2 Level 5 Stablize Stablize Example 6 3 Level 5 Stablize Stablize Example 7 4 Level 5 Stablize Stablize

[0128] The results in Table 3 show that when MCQ-803 is used as a wetting agent and the dosage of the wetting agent is 2 to 4 g, the wetting effect is good, and the hot storage stability and cold storage stability of the nano-seed treated suspension are good.

[0129] Example 8

[0130] The difference between this embodiment and embodiment 1 is that the weight content of the polyacrylic acid resin (0062) in this embodiment is 3 g, and the remaining raw materials and their weight contents are the same as those in embodiment 1.

[0131] The preparation method of the nano seed treatment suspension concentrate comprises the following specific steps:

[0132] The above-mentioned weights of metalaxyl-M, fludioxonil, thiabendazole, hyperbranched polymer dispersant (F031), phosphates (FS-3000), acetylene glycol polyoxyethylene ethers (MCQ-803), polyacrylic acid resins (0062), acid scarlet, glycerol, magnesium aluminum silicate (SF-04), cellulose (MC-S171), AF defoamer and water were weighed, mixed evenly, and then sand-milled in a sand mill to 200 nm to obtain a nano seed treatment suspension.

[0133] Example 9

[0134] The difference between this embodiment and embodiment 1 is that in this embodiment, 3 g of polyacrylic acid resin (KMM20) is used to replace 5 g of polyacrylic acid resin (0062) in embodiment 1, and the remaining raw materials and their weight contents are the same as those in embodiment 1.

[0135] The preparation method of the nano seed treatment suspension concentrate comprises the following specific steps:

[0136] The above-mentioned weights of metalaxyl-M, fludioxonil, thiabendazole, hyperbranched polymer dispersant (F031), phosphates (FS-3000), acetylene glycol polyoxyethylene ethers (MCQ-803), polyacrylic acid resins (KMM20), acid red, glycerol, magnesium aluminum silicate (SF-04), cellulose (MC-S171), AF defoamer and water were weighed, mixed evenly, and then sand-milled in a sand mill to 200 nm to obtain a nano seed treatment suspension.

[0137] Example 10

[0138] The difference between this embodiment and embodiment 1 is that in this embodiment, 5 g of polyacrylic acid resin (KMM20) is used to replace 5 g of polyacrylic acid resin (0062) in embodiment 1, and the remaining raw materials and their weight contents are the same as those in embodiment 1.

[0139] The preparation method of the nano seed treatment suspension concentrate comprises the following specific steps:

[0140] The above-mentioned weights of metalaxyl-M, fludioxonil, thiabendazole, hyperbranched polymer dispersant (F031), phosphates (FS-3000), acetylene glycol polyoxyethylene ethers (MCQ-803), polyacrylic acid resins (KMM20), acid red, glycerol, magnesium aluminum silicate (SF-04), cellulose (MC-S171), AF defoamer and water were weighed, mixed evenly, and then sand-milled in a sand mill to 200 nm to obtain a nano seed treatment suspension.

[0141] Test Example 4

[0142] Use a pipette to take 1 mL of the nano seed treatment suspension prepared in Example 1 and Examples 8 to 10, respectively, and add them to each culture dish. Then add 80 g of peanut seeds to each culture dish, cover and shake evenly for 5 minutes, open the lid, spread the peanut seeds flat to form a film, observe the surface of the peanut seeds, record the film formation time, and measure the coating uniformity and shedding rate. Among them, the film formation time is the time required for the nano seed treatment suspension on the surface of all wheat seeds to solidify into a film. The results are shown in Table 4.

[0143] Table 4 Results of film formation time, coating uniformity and shedding rate of peanut seeds treated with different nano seed treatment suspensions

[0144] Group Film former Film-forming agent addition amount / g Film forming time / min Uniformity / % Drop rate / % Example 8 0062 3 3 96.6 1.1 Example 1 0062 5 2 99.7 0.6 Example 9 KMM20 3 5 88.4 3.3 Example 10 KMM20 5 6 94.2 2.0

[0145] The results in Table 4 show that when the film-forming agent in the nano seed treatment suspension is 3 to 5 g of polyacrylic acid resin 0062 or 3 to 5 g of polyacrylic acid resin, the nano seed treatment suspension can achieve a higher coating uniformity and a lower coating shedding rate, and the film-forming time is shorter.

[0146] Comparative Example 9

[0147] The difference between this comparative example and Example 1 is that this comparative example uses 1g of magnesium aluminum silicate (SF-04) to replace 1g of magnesium aluminum silicate (SF-04) and 3g of cellulose (MC-S171) in Example 1, and the remaining raw materials and their weight contents are the same as those in Example 1.

[0148] The preparation method of the nano seed treatment suspension concentrate comprises the following specific steps:

[0149] The above-mentioned weights of metalaxyl-M, fludioxonil, thiabendazole, hyperbranched polymer dispersant (F031), phosphates (FS-3000), acetylene glycol polyoxyethylene ethers (MCQ-803), polyacrylic acid resins (0062), acid scarlet, glycerol, magnesium aluminum silicate (SF-04), AF defoamer and water were weighed, mixed evenly, and then sand-milled in a sand mill to 200 nm to obtain a nano seed treatment suspension.

[0150] Comparative Example 10

[0151] The difference between this comparative example and Example 1 is that this comparative example uses 3g of cellulose (MC-S171) to replace 1g of magnesium aluminum silicate (SF-04) and 3g of cellulose (MC-S171) in Example 1, and the remaining raw materials and their weight contents are the same as those in Example 1.

[0152] The preparation method of the nano seed treatment suspension concentrate comprises the following specific steps:

[0153] The above-mentioned weights of metalaxyl-M, fludioxonil, thiabendazole, hyperbranched polymer dispersant (F031), phosphates (FS-3000), acetylene glycol polyoxyethylene ethers (MCQ-803), polyacrylic acid resins (0062), acid red, glycerol, cellulose (MC-S171), AF defoamer and water were weighed, mixed evenly, and then sand-milled in a sand mill to 200 nm to obtain a nano seed treatment suspension.

[0154] Test Example 5

[0155] The nano seed treatment suspension of Comparative Examples 9-10 and Example 1 was divided into two equal parts, and stored at 0±2°C for 14 days (cold storage) and at 54±2°C for 14 days (hot storage), respectively. After being taken out, they were allowed to stand at room temperature for 3 hours to observe fluidity or stratification. The results are shown in Table 5.

[0156] Table 5 Fluidity and stability results of nanoseed treated suspensions containing different types of thickeners

[0157]

[0158]

[0159] The results in Table 5 show that when the thickener in the nano-seed treated suspension is 1g of SF-04 and 3g of MC-S171 by mass percentage, the nano-seed treated suspension has good fluidity and stability.

[0160] Test Example 6

[0161] The nano seed treatment suspension was taken for quality inspection, and the effects of the nano seed treatment suspension on the safety, germination rate and seedling quality of peanut seeds and the prevention effect on peanut root rot were investigated through field trials. Due to limited space, the quality inspection and field trials of the nano seed treatment suspension in Example 1 are used as an example to illustrate the nano seed treatment suspension in the embodiment of the present invention.

[0162] (1) Quality inspection of nano seed treatment suspension:

[0163] The nano seed treatment suspension in the above examples was subjected to quality inspection. The quality control standard of the nano seed treatment suspension was implemented according to HG / T 2467.1-2467.20-2003, GB 28132-2011 and NY 621-2002. The quality inspection results of the nano seed treatment suspension in Examples 1 to 10 all met the quality control standards.

[0164] Taking the nano seed treatment suspension concentrate of Example 1 as an example, the quality control standard, quality detection method and detection results of the nano seed treatment suspension concentrate of the examples of the present application are described below.

[0165] Five batches of nano seed treatment suspension samples of Example 1 were prepared according to the nano seed treatment suspension preparation method, and the five batches of samples were tested respectively. The quality control standards of the nano seed treatment suspension are shown in Table 6.

[0166] Table 6 Quality control standards for nano seed treatment suspension

[0167]

[0168] The content of active ingredients was determined by high performance liquid chromatography using a Hypersil C18 column. The chromatographic conditions were as follows: injection volume of 5.0 μL; column temperature of 35°C; detection wavelength of 273 nm; filtration through a 0.45 μm pore size membrane, ultrasonic degassing in an ultrasonic bath for 15 min, and a mixture of acetonitrile and water in a volume ratio of 25:75 as the mobile phase. The retention times of metalaxyl-M, fludioxonil and thiabendazole were approximately 7.66 min, 10.12 min and 16.36 min, respectively.

[0169] The pH value was determined in accordance with GB / T 1601-1993.

[0170] Persistent foaming test: Conduct according to 4.11 of HG / T 2467.5-2003.

[0171] Low temperature stability test: Conduct according to 4.10 of HG / T 2467.2-2003. After slight agitation, there should be no visible particles or oily matter.

[0172] Hot storage stability test: Conduct according to 4.11 of HG / T 2467.2-2003.

[0173] The film-forming property, coating uniformity and coating shedding rate were determined in accordance with NY 621-2002.

[0174] After testing, the test results of the above 5 batches of nano seed treatment suspension are detailed in Table 7. The test results of the 5 batches of nano seed treatment suspension are in line with the quality control standards in Table 6.

[0175] Table 7 Test results of 5 batches of nano seed treatment suspension

[0176]

[0177] The results in Table 7 show that the formula and preparation method of the nano seed treatment suspension of the present invention are feasible, the product quality of the nano seed treatment suspension is stable, meets the relevant quality standards, and has small batch differences and good method reproducibility.

[0178] (2) Field safety and efficacy tests

[0179] Field trials were conducted with peanut (Jihua No. 7) as the test crop to investigate the safety of the nano seed treatment suspension of Example 1 on peanuts and its effects on the germination rate, emergence rate, disease conditions and seedling quality of peanuts, as well as the control effect of the nano seed treatment suspension on peanut root rot, and to provide a scientific basis for the promotion and application of the nano seed treatment suspension in disease prevention technology in cold-region peanut fields.

[0180] Test conditions:

[0181] Experimental crop: Peanut (Jihua No. 7).

[0182] Test site and cultivation conditions: The test site was set up at the Yanjiagang Farm Test Base of the Institute of Plant Protection, Heilongjiang Academy of Agricultural Sciences. The test soil type was slightly swampy meadow soil with an organic matter content of about 3% and a pH of 6.3.

[0183] Target of prevention and control: Peanut root rot (Fusarium moniliforme Sheld.).

[0184] Test agents: the nano seed treatment suspension of Example 1 and commercially available 18% thiabendazole-methyl·thiabendazole FS (purchased from Syngenta, common seed treatment suspension).

[0185] Application method: The nano seed treatment suspension prepared in Example 1 was used as the test agent, and the commercially available 18% thiabendazole-methyl FS was used as the control agent. Both of them were peanut coating agents. During coating, the test agent and the control agent were mixed with the seeds in a barrel for coating.

[0186] Experimental process: There are 5 treatment groups as shown in Table 8, each treatment group has 3 replicates, each replicate has 4 plates (0.167m 2 / tray), the sowing amount was 120 g / tray, the seeds were soaked and germinated before sowing, and the peanut seeds were coated on April 10, 2024, soaked (20°C) on April 12, germinated on April 14, sown on April 17, seeded on April 24, and plant analysis was performed on May 9. Among them, when the peanut seeds of treatment groups 1-4 were coated, the above-mentioned nano seed treatment suspension and the commercially available 18% thiabendazole FS were diluted with water to prepare a coating solution, so that the weight ratio of the coating solution to the seeds was 1:80.

[0187] Table 8 Test agents and dosages in different groups

[0188] Treatment Group Test drug Dosage (g / 100kg seeds) 1 Nano seed treatment suspension 100 2 Nano seed treatment suspension 150 3 Commercially available 18% thiabendazole-methyl-pyridoxine-methyl FS 100 4 Commercially available 18% thiabendazole-methyl-pyridoxine-methyl FS 150 5 Blank control (clear water) ——

[0189] In the experiment, treatment groups 1 and 2 were used as experimental treatment groups, treatment groups 3 and 4 were used as control treatment groups, and treatment group 5 was used as a blank control treatment group. Safety, germination rate, incidence rate, and seedling quality were investigated, as well as the prevention effect on peanut root rot. The specific investigation methods are as follows:

[0190] Safety investigation: Observe whether the peanut seedlings emerge evenly and whether there are any deformities, twists, etc. after emergence.

[0191] Germination rate investigation: When sowing, a certain amount of seeds in each treatment group were set aside for germination, and the germination rate was investigated.

[0192] Incidence rate and prevention efficacy survey: Peanut root rot was investigated at the 2-leaf stage. The survey method was to visually inspect the entire area of ​​each treatment, investigate the number of diseased plants, and calculate the diseased plant rate and prevention efficacy according to the following formula.

[0193] Disease rate (%) = number of diseased plants ÷ total number of surveyed plants × 100%.

[0194] Prevention effect (%) = (ck-pt) ÷ ck × 100%.

[0195] In the above formula, ck is the diseased plant rate of the blank control treatment group, and pt is the diseased plant rate of the investigated treatment group.

[0196] Peanut seedling quality survey: The plant height, leaf age, root number, above-ground dry weight, underground dry weight, stem base width and fullness of peanut seedlings in different treatment groups were compared and analyzed at the 3.1-3.5 leaf stage. The specific method was to take about 100 plants from each treatment group, wash off the soil on the roots and tie them up for analysis.

[0197] The results of the field trials are as follows:

[0198] Safety Survey Results:

[0199] The peanuts in each treatment group showed uniform seedlings without deformed seedlings, and each treatment group was safe for peanuts. Among them, the control treatment groups (treatment groups 3 and 4) emerged slightly slower than the other treatment groups, but performed normally in the later stage without any impact. The experimental treatment groups (treatment groups 1 and 2) emerged uniformly without deformities or twists, and the experimental treatment groups performed normally. The nano seed treatment suspension of the embodiment of the present invention is safe for peanuts.

[0200] Germination rate survey results: A germination rate survey of peanut seeds was conducted on April 20. The results are shown in Table 9.

[0201] Table 9 Survey results of germination rate in different treatment groups

[0202]

[0203] The germination rate survey shows that the germination speed of each treatment group is different, but the difference between the same treatment groups is not much. As shown in Table 9, among the 5 treatment groups, the peanut germination rate of treatment group 1 with a nano seed treatment suspension of 100g / 100kg seeds is the highest, at 98.4%, and there are significant differences between treatment group 1 and treatment groups 2, 3 and 4. The germination rate of treatment group 1 with a nano seed treatment suspension of 100g / 100kg seeds is not significantly different from that of the blank control group.

[0204] Morbidity and efficacy survey results:

[0205] The survey results of the incidence and control efficacy of peanut root rot in the above five treatment groups are shown in Table 10.

[0206] Table 10 Survey results on the incidence and control efficacy of peanut root rot in different treatment groups

[0207] Treatment Group Number of diseased plants / area Number of diseased plants / tray Incidence (%) Prevention effect (%) 1 <![CDATA[0.3 cC ]]> <![CDATA[0.08 cC ]]> <![CDATA[0.0021 cC ]]> <![CDATA[99.7 aA ]]> 2 <![CDATA[0.0 cC ]]> <![CDATA[0.00 cC ]]> <![CDATA[0.00 cC ]]> <![CDATA[100.0 aA ]]> 3 <![CDATA[0.0 bB ]]> <![CDATA[0.00 bB ]]> <![CDATA[0.00 bB ]]> <![CDATA[85.01 bB ]]> 4 <![CDATA[3.5 bB ]]> <![CDATA[0.905 bB ]]> <![CDATA[0.0233 bB ]]> <![CDATA[85.02 bB ]]> 5 <![CDATA[148 aA ]]> <![CDATA[37.00 aA ]]> <![CDATA[0.9250 aA ]]> ——

[0208] Note: Different letters between any two treatment groups indicate significant differences, and the same letters indicate no significant differences.

[0209] As shown in Table 10, among the treatment groups 1 to 5, the number of diseased plants in the whole area, the number of diseased plants per tray and the incidence rate of the blank control treatment group are the highest, and the number of diseased plants in the whole area, the number of diseased plants per tray and the incidence rate of the blank control treatment group are significantly different from those of the other treatment groups. There are no significant differences in the number of diseased plants in the whole area, the number of diseased plants per tray, the incidence rate and the prevention effect between the experimental treatment groups (treatment group 1 and treatment group 2), and the prevention effect of the experimental treatment groups on peanut root rot is about 100%. There are no significant differences in the number of diseased plants in the whole area, the number of diseased plants per tray, the incidence rate and the prevention effect of the control treatment groups (treatment group 3 and treatment group 4), and the prevention effect of the control treatment groups is about 85%. There is a significant difference between the prevention effect of the experimental treatment group on peanut root rot and the control treatment group, and the prevention effect of the experimental treatment on peanut root rot is significantly greater than that of the control treatment group, indicating that the nano seed treatment suspension provided in the embodiment of the present application has a significant prevention and control effect on peanut root rot.

[0210] Field test results:

[0211] Peanuts in all treatments showed uniform seedlings, no deformed seedlings and buds, and were safe for peanut growth. The treatment with commercially available 18% thiabendazole FS had slower seedling emergence than other treatments, shorter plant height, and normal performance in the later stage.

[0212] The nano seed treatment suspension of the embodiment of the present application is used to coat peanut seeds, and the prevention effect on peanut root rot is very good, with a prevention effect of more than 99.7%. The commercially available 18% metalaxyl·fluoxetine·thiabendazole FS is used to coat peanut seeds, and the prevention effect on peanut root rot is about 85%. The prevention effect of the nano seed treatment suspension of the embodiment of the present application on peanut root rot is significantly better than the commercially available 18% metalaxyl·fluoxetine·thiabendazole FS, indicating that the nano seed treatment suspension of the embodiment of the present application is compounded with metalaxyl, fludioxonil, thiabendazole, dispersant, wetting agent, antifreeze agent, thickener, film-forming agent, warning agent, defoaming agent and water, and is processed into a nano preparation through a nano processing technology, which can be more conducive to the exertion of the efficacy of the active ingredients, enhance the efficacy of the active ingredients, and improve the prevention and control effect of the nano seed treatment suspension on peanut root rot.

[0213] Except for the blank control treatment group, there were no significant differences in the survey results of various survey items of peanut seedling quality among the treatment groups, and the nano seed treatment suspension of the embodiment of the present application had no adverse effects on various survey items of peanut seedling quality.

[0214] After comprehensively analyzing the safety of peanuts, the prevention effect of peanut root rot and the quality of peanut seedlings obtained from the experiments of each treatment group, the use of the nano seed treatment suspension of the embodiment of the present application for coating at 150g / 100kg to 200g / 100kg of seeds can effectively prevent and control peanut root rot.

[0215] The nano seed treatment suspensions of Example 2 and Example 3 were used for field tests, and the results also showed that the nano seed treatment suspensions of Example 2 and Example 3 had no significant effect on the safety of peanuts and the germination rate of peanut seeds, and had no adverse effects on various survey items of peanut seedling quality, and had good preventive and control effects on peanut root rot.

[0216] The above field test shows that peanut root rot can be effectively prevented and controlled by treating peanut seeds with the nano seed treatment suspension of the embodiment of the present application. No additional fungicide is needed to prevent and control peanut root rot in the early and middle stages of peanut growth. At the same time, peanut seeds are treated at a dosage of 100g / 100kg to 200g / 100kg of seeds. After peanuts are planted, there is basically no need to prevent and control peanut root rot within 90 days, and there is no adverse effect on peanut growth. This solves the technical problem that ordinary seed treatment agents such as seed treatment suspensions in the prior art have poor control effects on peanut root rot, low safety, need to be assisted by foliar spray, and need to apply pesticides multiple times during the growth period of peanuts to effectively control the hazards of diseases, which consumes a lot of manpower and material resources. The nano seed treatment suspension of the embodiment of the present application can effectively reduce the amount and frequency of pesticide use by reducing the particle size of the dispersed phase particles, increasing permeability, and improving the utilization rate of active ingredients, as well as enhancing the efficacy of active ingredients through compounding.

[0217] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A nano seed treatment suspending agent, characterized in that: The method is composed of the following raw materials in parts by weight: 0.1-10 parts of metalaxyl-M, 0.1-10 parts of fludioxonil, 0.1-30 parts of thiabendazole, 1-15 parts of dispersant, 2-5 parts of wetting agent, 3-12 parts of film-forming agent, 6-14 parts of warning agent, 5-7 parts of antifreeze agent, 3-5 parts of thickener, 0.2-0.8 parts of defoaming agent, and water is added to 100 parts; The dispersant is a hyperbranched polymer dispersant and / or a phosphate; The thickener is magnesium aluminum silicate and cellulose, and the mass ratio of magnesium aluminum silicate to cellulose is 0.5 to 1.5:3; The wetting agent is acetylenic glycol polyoxyethylene ether.

2. The nano seed treatment suspending agent according to claim 1, characterized in that The nano seed treatment suspension is composed of the following raw materials in parts by weight: 1-7 parts of metalaxyl-M, 0.5-3 parts of fludioxonil, 5-20 parts of thiabendazole, 3-10 parts of dispersant, 2-4 parts of wetting agent, 3-12 parts of film-forming agent, 6-14 parts of warning agent, 5-7 parts of antifreeze agent, 3-5 parts of thickener, 0.2-0.8 parts of defoaming agent, and water is added to 100 parts.

3. The nano seed treatment suspending agent according to claim 1, characterized in that The hyperbranched polymer dispersant is hyperbranched polymer dispersant F031, the phosphate is phosphate FS-3000, when the dispersant is a hyperbranched polymer dispersant and a phosphate, the mass ratio of the hyperbranched polymer dispersant to the phosphate is 2-4:3-4; the acetylene glycol polyoxyethylene ether is acetylene glycol polyoxyethylene ether MCQ-803; the cellulose is cellulose MC-S171.

4. The nano seed treatment suspending agent according to claim 1, characterized in that The film-forming agent includes polyacrylic acid resins; the warning agent includes at least one of acid scarlet and alkaline rose essence; the antifreeze agent includes at least one of propylene glycol and ethylene glycol; and the defoaming agent includes AF defoaming agent.

5. The nano seed treatment suspending agent according to claim 4, characterized in that The polyacrylic acid resin is polyacrylic acid resin 0062.

6. The nano seed treatment suspension according to any one of claims 1 to 5, characterized in that: The particle size of the nano seed treatment suspending agent is 200-500nm.

7. The method for preparing the nano seed treatment suspension according to any one of claims 1 to 6, characterized in that: The following steps are involved: The metalaxyl-M, fludioxonil, thiabendazole, dispersant, wetting agent, film-forming agent, warning agent, antifreeze agent, thickener, defoaming agent and water are mixed evenly and then sand-milled to obtain a nano seed treatment suspension.

8. The preparation method according to claim 7, characterized in that: The sand is ground to a particle size of 200 to 500 nm.

9. Use of the nano seed treatment suspension according to any one of claims 1 to 6 or the preparation method according to claim 7 or 8 in promoting plant growth, characterized in that: The plant growth traits include one or more of plant seedling uniformity, absence of deformed seedlings, plant height and plant germination rate.

10. Use of the nano seed treatment suspension according to any one of claims 1 to 6 or the preparation method according to claim 7 or 8 in preventing and controlling plant root rot.

Images