Pesticide composition containing Sulfiflumin and application thereof

Abstract

The present invention relates to pesticide compositions containing Sulfiflumin and LHP-560 or trifluorocide, and methods for their use in the control of pests and mites in agronomic and non-agronomic environments. The synergistic composition has a synergistic effect, the pest control effect can be improved, the insecticidal spectrum can be expanded, field pests occur in a mixed manner, and the effect on pests is stronger; and the two effective components have different action mechanisms, so that the pesticide resistance of pests can be delayed when the two effective components are mixed for use, the pesticide application amount can be reduced, the use frequency can be reduced, and the use cost can be reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide compounding, and particularly relates to a pesticide composition containing Sulfiflumin and LHP-560 or trifluoxetine, and a method for using the pesticide composition to prevent and control pests and mites in agricultural and non-agricultural environments. Background Art

[0002] In recent years, the occurrence of various crop pests has shown a trend of increasing year by year, and the area of ​​occurrence has increased year by year, causing great harm to crops, resulting in crop yield reduction, increased agricultural production costs and other problems. In order to improve the control effect of pests, farmers increase the dosage of pesticides or mix pesticides at will. This unscientific use of pesticides not only fails to achieve synergistic effects, but also leads to waste of pesticides, excessive residues, environmental pollution, and pests' resistance to pesticides. Therefore, it is urgent to develop a synergistic composition for pests that can achieve the effects of expanding the scope of control targets, reducing the dosage of pesticides, and delaying the development of resistance.

[0003] In the actual process of agricultural production, if the same pesticide is used continuously for a long time, pests will soon develop resistance, resulting in reduced control effectiveness, increased pesticide use, and aggravated pesticide residues in agricultural products and damage to the ecological environment. Compounding pesticides with completely different mechanisms of action is an effective way to delay the development of pest resistance, expand the insecticide spectrum, extend the service life, and reduce the amount of pesticides used. However, how to compound so that the drugs produce synergy rather than antagonism is a major difficulty.

[0004] Sulfiflumin, alias: (2Z)-2-[[2-fluoro-4-methyl-5-[(R)-(2,2,2-trifluoroethyl)sulfinyl]phenyl]imino]-3-(2,2,2-trifluoroethyl)-4-thiazolidinone[2377084-09-6]; molecular formula: C 14 H 11 F7N2O2S2; relative molecular mass: 436.368, its structural formula is as follows:

[0005] Summary of the invention

[0006] In order to improve the deficiencies of the prior art, the object of the present invention is to provide a synergistic composition containing Sulfiflumin and its application, wherein the composition comprises an active ingredient, wherein the active ingredient comprises a compound I and a compound II, wherein the compound I is selected from Sulfiflumin, and the compound II is selected from one of LHP-560 and trifluoxetine or a mixture thereof;

[0007] The structural formula of compound I is shown below:

[0008]

[0009] The structural formula of LHP-560 is shown below:

[0010]

[0011] According to an embodiment of the present invention, the compound LHP-560 is prepared by referring to the method of Example 1 in patent document CN 111909143 A, the entire text of which is incorporated herein by reference.

[0012] According to an embodiment of the present invention, the mass ratio of compound I to compound II is 80-1:1-80.

[0013] According to an embodiment of the present invention, the mass ratio of compound I to compound II is 50-1:1-50.

[0014] According to a preferred embodiment of the present invention, the mass ratio of compound I to compound II is 10-1:1-10.

[0015] As an example, the mass ratio of compound I to compound II is 80:1, 75:1, 70:1, 65:1, 60:1, 55:1, 50:1, 45:1, 40:1, 35:1, 30:1, 25:1, 20:1, 15:1, 10:1, 5:1, 2:1, 3:2, 1:1, 1:2, 2:3, 1:5, 1:10, 1:15, 1:20, 1:25, 1:30, 1:35, 1:40, 1:45, 1:50, 1:55, 1:60, 1:65, 1:70, 1:75, and 1:80.

[0016] According to an embodiment of the present invention, the synergistic composition comprises Sulfiflumin and LHP-560.

[0017] According to an embodiment of the present invention, the synergistic composition comprises Sulfiflumin and trifloxystrobin.

[0018] According to an embodiment of the present invention, the synergistic composition comprises Sulfiflumin, LHP-560 and trifloxystrobin.

[0019] According to an embodiment of the present invention, based on the total weight of the synergistic composition as 100%, the sum of the mass of the compound I and the compound II in the composition is 1-80%, and the rest is excipients; preferably 2-60%, for example 1%, 5%, 8%, 10%, 13%, 15%, 18%, 20%, 25%, 30%, 35%, 38%, 42%, 45%, 50%, 55%, 60%, 65%, 68%, 75%.

[0020] According to an embodiment of the present invention, the synergistic composition can be prepared as a liquid preparation or a solid preparation.

[0021] According to the embodiment of the present invention, the content range of the effective active ingredient in the preparation varies according to different preparation types. Generally, the liquid preparation contains 1% to 60% of the effective active ingredient by weight, and the rest is excipients, preferably 5% to 50%; the solid preparation contains 5% to 80% of the effective active ingredient by weight, and the rest is excipients, preferably 10% to 70%.

[0022] According to an embodiment of the present invention, the synergistic composition also includes excipients, and the excipients include at least one of the following: deionized water, organic solvents, emulsifiers, dispersants, wetting agents, thickeners, defoamers, stabilizers, binders, disintegrants, antifreeze agents, anticaking agents, suspending agents, film-forming agents, preservatives, colorants, polymer capsule wall materials, pH regulators or fillers.

[0023] According to an embodiment of the present invention, the synergistic composition can be diluted or used directly by the user before use. The preparation can be prepared by a processing method known to those skilled in the art, that is, the effective active ingredient is mixed with one or more of the auxiliary materials such as deionized water, organic solvents, emulsifiers, dispersants, wetting agents, thickeners, defoamers, stabilizers, binders, disintegrants, antifreeze agents, anticaking agents, suspending agents, film formers, preservatives, colorants, polymer capsule wall materials, pH regulators or fillers to prepare the synergistic composition.

[0024] According to the preparation provided by the present invention, the synergistic composition can be made into various dosage forms. Preferably, the dosage forms include water-dispersible granules, dispersible liquids, wettable powders, suspensions, aqueous emulsions, microemulsions, suspoemulsions, microcapsule suspensions, microcapsule suspension-suspension concentrates, suspension seed coatings, emulsifiable concentrates, and granules.

[0025] According to an embodiment of the present invention, the synergistic composition comprises the following components and contents when made into a wettable powder: 1% to 80% of compound I, 1% to 80% of compound II, 1% to 12% of dispersant, 1% to 8% of wetting agent, and the balance is filler.

[0026] Preferably, the compound I in the synergistic composition is 2% to 70%, 5% to 65%, 10% to 60%, 15% to 50%, etc.;

[0027] Compound II is 2% to 70%, 5% to 65%, 10% to 60%, 15% to 50%, etc.;

[0028] The dispersant is 1% to 8%, 2% to 7%, 3% to 6% or 4% to 5%;

[0029] The wetting agent is 1% to 8%, 2% to 7%, 3% to 6% or 4% to 5%.

[0030] According to an embodiment of the present invention, the synergistic composition comprises the following components and contents when prepared into water-dispersible granules: compound I 1% to 80%, compound II 1% to 80%, dispersant 1% to 12%, wetting agent 1% to 8%, disintegrant 1% to 10%, and the balance is filler.

[0031] Preferably, the compound I in the synergistic composition is 2% to 70%, 5% to 65%, 10% to 60%, 15% to 50%, etc.;

[0032] Compound II is 2% to 70%, 5% to 65%, 10% to 60%, 15% to 50%, etc.;

[0033] The dispersant is 1% to 8%, 2% to 7%, 3% to 6% or 4% to 5%;

[0034] The wetting agent is 1% to 8%, 2% to 7%, 3% to 6% or 4% to 5%;

[0035] The disintegrant is 1% to 18%, 2% to 16%, 3% to 15%, 4% to 12%, 5% to 10% or 6% to 8%.

[0036] According to an embodiment of the present invention, the synergistic composition comprises the following components and contents when prepared as a suspension: compound I 1% to 50%, compound II 1% to 50%, dispersant 1% to 12%, wetting agent 1% to 10%, thickener 0.1% to 8%, antifreeze agent 0.1% to 8%, and the balance is deionized water.

[0037] Preferably, the compound I in the synergistic composition is 2% to 45%, 5% to 40%, 10% to 35%, 15% to 30%, 20% to 25%, etc.;

[0038] Compound II is 2% to 45%, 5% to 40%, 10% to 35%, 15% to 30%, 20% to 25%, etc.;

[0039] Dispersant is 1% to 4% or 2% to 3%;

[0040] The wetting agent is 1% to 8%, 2% to 7%, 3% to 6% or 4% to 5%;

[0041] Thickener: 0.1% to 0.25%;

[0042] Antifreeze 1%~4% or 2%~3%.

[0043] According to an embodiment of the present invention, the synergistic composition comprises the following components and contents when prepared as an aqueous emulsion: compound I 1% to 50%, compound II 1% to 50%, organic solvent 1% to 60%, emulsifier 1% to 12%, antifreeze agent 0.1% to 8%, defoamer 0.01% to 2%, thickener 0.1% to 2%, and the balance is deionized water.

[0044] Preferably, the compound I in the synergistic composition is 2% to 45%, 5% to 40%, 10% to 35%, 15% to 30%, 20% to 25%, etc.;

[0045] Compound II is 2% to 45%, 5% to 40%, 10% to 35%, 15% to 30%, 20% to 25%, etc.;

[0046] The organic solvent is 1% to 90%, 2% to 80%, 5% to 70%, 10% to 60%, 15% to 50%, 20% to 50%, 30% to 40%, etc.;

[0047] Emulsifier 1% to 8%, 2% to 7%, 3% to 6% or 4% to 5%;

[0048] Antifreeze 1% to 7% or 2% to 6%;

[0049] Defoaming agent is 0.05%~1.5%, thickener is 0.1%~1.8%, 0.2%~1.5%.

[0050] According to an embodiment of the present invention, the synergistic composition comprises the following components and contents when prepared as a suspended seed coating agent: 1% to 50% of compound I, 1% to 50% of compound II, 1% to 12% of dispersant, 1% to 10% of wetting agent, 1% to 10% of antifreeze agent, 0.1% to 10% of anticaking agent, 0.1% to 5% of suspending agent, 1% to 10% of film-forming agent, 0.1% to 5% of preservative, 1% to 30% of colorant, 0.1% to 5% of pH adjuster, 0.1% to 8% of thickener, and the balance is deionized water.

[0051] Preferably, the compound I in the synergistic composition is 2% to 45%, 5% to 40%, 10% to 35%, 15% to 30%, 20% to 25%, etc.;

[0052] Compound II is 2% to 45%, 5% to 40%, 10% to 35%, 15% to 30%, 20% to 25%, etc.;

[0053] The dispersant is 2% to 10%, 3% to 8%, 4% to 7%, or 5% to 6%;

[0054] The wetting agent is 1% to 8%, 2% to 7%, 3% to 6% or 4% to 5%;

[0055] Antifreeze agent is 1% to 8%, 2% to 7%, 3% to 6% or 4% to 5%;

[0056] The anticaking agent is 0.5% to 9%, 1% to 8%, 2% to 7%, 3% to 6% or 4% to 5%;

[0057] The suspending agent is 0.2-4%, 0.5%-3.5%, 1%-3%, 1.5%-2.5%, or 1.8%-2%;

[0058] The film-forming agent is 2% to 9%, 3% to 8%, 4% to 7%, or 5% to 6%;

[0059] The preservative is 0.2% to 4%, 0.5% to 3.5%, 1% to 3%, 1.5% to 2.5%, or 1.8% to 2%;

[0060] The colorant is 2% to 25%, 3% to 20%, 5% to 15%, 8% to 12%, or 9% to 10%;

[0061] pH adjuster 0.1% to 5%, 0.2% to 4%, 0.5% to 3.5%, 1% to 3%, 1.5% to 2.5%, or 1.8% to 2%;

[0062] The thickener is 0.5% to 7, 1% to 6%, 2% to 5%, or 3% to 4%.

[0063] According to an embodiment of the present invention, the synergistic composition comprises the following components and contents when prepared into a microcapsule suspension: 1% to 50% of compound I, 1% to 50% of compound II, 1% to 30% of polymer capsule wall material, 2% to 10% of dispersant, 1% to 50% of organic solvent, 1% to 7% of emulsifier, 0.1% to 5% of pH regulator, 0.01% to 2% of defoamer, 0.1% to 8% of thickener, 0.1% to 8% of antifreeze agent, and the balance is deionized water.

[0064] Preferably, the compound I in the synergistic composition is 2% to 45%, 5% to 40%, 10% to 35%, 15% to 30%, 20% to 25%, etc.;

[0065] Compound II is 2% to 45%, 5% to 40%, 10% to 35%, 15% to 30%, 20% to 25%, etc.;

[0066] Polymer capsule wall material 2-25%, 5-20%, 6-18%, or 10-15%, etc.;

[0067] The dispersant is 3% to 8%, 4% to 7%, or 5% to 6%;

[0068] The organic solvent is 2% to 45%, 5% to 40%, 10% to 35%, 15% to 30%; or 20% to 25%, etc.;

[0069] The emulsifier is 2% to 6% or 4% to 5%;

[0070] pH adjuster 0.2-4%, 0.5%-3.5%, 1%-3%, 1.5%-2.5%, or 1.8%-2%;

[0071] Defoaming agent is 0.05-1.8%, 0.1%-1.5%, 0.5%-1.3%, 0.8%-1.0%;

[0072] Thickener is 0.2% to 7%, 0.5% to 6%, 1% to 5%, 1.5% to 4% or 2% to 3%;

[0073] The antifreeze agent is 0.5% to 7%, 1% to 6%, 2% to 5% or 3% to 4%.

[0074] According to an embodiment of the present invention, the synergistic composition comprises the following components and contents when prepared into a microcapsule suspension-suspension agent: 1% to 50% of compound I, 1% to 50% of compound II, 1% to 12% of polymer capsule wall material, 1% to 12% of dispersant, 1% to 8% of wetting agent, 1% to 50% of organic solvent, 1% to 8% of emulsifier, 0.01% to 2% of defoaming agent, 0.1% to 8% of thickener, 0.1% to 8% of pH regulator, 0.1% to 5% of antifreeze agent, and the balance is deionized water.

[0075] According to an embodiment of the present invention, the synergistic composition comprises the following components and contents when prepared into an emulsifiable concentrate: 1% to 50% of compound I, 1% to 50% of compound II, 1% to 50% of an organic solvent, 1% to 30% of an emulsifier, 1% to 10% of an antifreeze agent, 0.1% to 5% of a stabilizer, and the balance being an organic solvent.

[0076] Alternatively, the compound I is 0.1% to 50%, the compound II is 0.1% to 50%, the emulsifier is 0 to 12%, the stabilizer is 0% to 0.5%, and the balance is an organic solvent.

[0077] According to an embodiment of the present invention, the synergistic composition comprises the following components and contents when prepared as a microemulsion: 1% to 50% of compound I, 1% to 50% of compound II, 1% to 50% of an organic solvent, 1% to 30% of an emulsifier, 1% to 10% of an antifreeze agent, 0.1% to 5% of a stabilizer, and the remainder being deionized water.

[0078] According to an embodiment of the present invention, the synergistic composition comprises the following components and contents when prepared as a dispersible liquid: 1% to 50% of compound I, 1% to 50% of compound II, 1% to 30% of emulsifier, 1% to 10% of antifreeze agent, 0.1% to 5% of stabilizer, and the balance is an organic solvent.

[0079] According to an embodiment of the present invention, the composition comprises the following components and contents when made into granules: compound I 0.5% to 10%, compound II 0.5% to 10%, dispersant 1% to 12%, wetting agent 1% to 10%, binder 0.1% to 8%, and the balance is filler.

[0080] According to an embodiment of the present invention, the emulsifier can be selected from one of the following: sodium lignin sulfonate, agricultural milk, phenylphenol polyoxyethylene ether phosphate, tristyrylphenol polyoxyethylene ether phosphate triethanolamine salt, benzyl dimethylphenol polyoxyethyl ether, anhydrous sorbitan fatty acid ester polyoxyethylene ether, sorbitan anhydride oleate (Span-80), fatty alcohol polyoxyethylene ether, sodium alkyl naphthalene sulfonate, sodium isooctyl succinate sulfonate, nonylphenol polyoxyethylene ether phosphate, castor oil polyoxyethylene ether phosphate.

[0081] According to an embodiment of the present invention, the dispersant can be selected from: glycerol fatty acid polyoxyethylene ether, polyoxyethylene alkyl aryl ether, sodium lignin sulfonate, naphthalene sulfonate formaldehyde condensation product, fatty alcohol polyoxyethylene ether sulfate, naphthalene sulfonate formaldehyde condensation product, naphthalenesulfonic acid formaldehyde condensation product sodium salt, nonylphenol polyoxyethylene ether, polyoxyethylene lanolin alcohol, alkylphenol polyoxyethylene ether formaldehyde condensation product, fatty alcohol polyoxyethylene ether, fatty alcohol polyoxyethylene ether phosphate ester, polyoxyethylene sorbitan fatty acid ester, phosphate ester or more.

[0082] According to an embodiment of the present invention, the wetting agent can be selected from: trisiloxane polyoxyethylene ether, sodium N-lauroyl glutamate, sodium lauryl sulfate, sodium lauroyl sarcosinate, sodium methylnaphthalene sulfonate formaldehyde condensate, castor oil polyoxyethylene ether, triphenyl ethyl phenol polyoxyethylene ether, sodium dodecylbenzene sulfonate, sodium alkyl naphthalene sulfonate, sodium isooctyl succinate sulfonate, polyoxyethylene alkyl aryl ether, fatty alcohol polyether glycerol fatty acid polyoxyethylene ether, fatty alcohol polyoxyethylene ether or more.

[0083] According to an embodiment of the present invention, the binder can be selected from one or more of: xanthan gum, starch, urea-formaldehyde resin, gelatin, gum arabic, carboxymethyl cellulose, carboxyethyl cellulose, and polyvinyl alcohol.

[0084] According to an embodiment of the present invention, the disintegrant can be selected from one or more of sodium bicarbonate, ammonium sulfate, sodium sulfate, calcium sulfate, and magnesium chloride.

[0085] According to an embodiment of the present invention, the thickener may be selected from one or more of magnesium aluminum silicate, polyvinyl acetate, xanthan gum, gelatin, gum arabic, and polyvinyl alcohol.

[0086] According to an embodiment of the present invention, the defoaming agent can be selected from one or more of silicone oil, n-octanol, silicone, butyl phosphate, isobutyl phosphate and the like.

[0087] According to an embodiment of the present invention, the antifreeze agent can be selected from one or more of propylene glycol, ethylene glycol, glycerol, etc.

[0088] According to an embodiment of the present invention, the stabilizer can be selected from one or more of triethanolamine, epichlorohydrin, butyl glycidyl ether, triphenyl phosphite, N-soybean oil-based trimethylene diamine, dialkyl succinic acid acetate sulfonate, and the like.

[0089] According to an embodiment of the present invention, the filler includes a solid filler and a liquid filler, wherein the solid filler can be selected from one or more of kaolin, attapulgite, diatomaceous earth, white carbon black, bentonite, montmorillonite, calcium carbonate, and talc. The liquid filler can be selected from one or more of soybean oil, castor oil, and mineral oil.

[0090] According to an embodiment of the present invention, the organic solvent can be selected from: ethyl acetate, acetone, isopropanol, 2,2,2-trifluoroethanol, propylene carbonate, benzene, toluene, xylene, dimethylformamide, dimethyl sulfoxide, dichloromethane, cyclohexane, cyclohexanone, N-methylpyrrolidone, solvent oil (such as 150# solvent oil) or one or more thereof.

[0091] The present invention also provides the use of the synergistic composition for controlling pests and mites in agriculture and non-agriculture.

[0092] According to an embodiment of the present invention, the pests are pests of the order Lepidoptera, Hemiptera, Coleoptera, Thysanoptera or Diptera.

[0093] As an embodiment, the pest is selected from Plutella xylostella.

[0094] As an embodiment, the mite is selected from Panonychus citri, Tetranychus urticae or Tetranychus cinnabarinus.

[0095] The present invention also provides a method for controlling agricultural or non-agricultural pests and mites, comprising applying the synergistic composition as described above to plants where pests and mites exist.

[0096] The synergistic composition of the present invention can be provided in the form of a finished preparation or a single dosage form, which can be directly mixed before use, and then mixed with water to a desired concentration, and can be applied to crops in any manner, such as spraying, root irrigation, and painting. In specific applications, it can also be mixed with other agents such as growth regulators, soil regulators, herbicides, nematicides, etc.

[0097] The present invention also provides a composition for controlling invertebrate pests, which comprises a biologically effective amount of the synergistic composition as described above and at least one other component selected from a surfactant, a solid diluent and a liquid diluent, and the composition optionally contains an effective amount of at least one other biologically active compound or active agent.

[0098] As an embodiment, the invertebrate pest is selected from Plutella xylostella. The present invention also provides a method for controlling invertebrate pests, which comprises contacting the invertebrate pests or their environment with a biologically effective amount of the composition for controlling invertebrate pests as described above.

[0099] The present invention also provides a spray composition, which comprises the synergistic composition of the present invention and a propellant.

[0100] The present invention also provides a bait composition, which comprises the synergistic composition of the present invention as described above; one or more food materials; optionally an insect attractant; and optionally a wetting agent.

[0101] The synergistic composition of the present invention has the following advantages:

[0102] 1) It has a synergistic effect, which can improve the pest control effect; 2) It expands the insecticide spectrum, and the insect pests in the field are often mixed, which has a stronger effect on the pests; 3) The two active ingredients have different mechanisms of action, and the mixed use can delay the development of pest resistance; 4) It reduces the amount of pesticide applied, reduces the number of uses, and reduces the cost of use. DETAILED DESCRIPTION

[0103] The present invention will be described in further detail below in conjunction with specific embodiments. It should be understood that the following embodiments are only exemplary descriptions and explanations of the present invention and should not be construed as limiting the scope of protection of the present invention. All technologies implemented based on the above content of the present invention are included in the scope that the present invention is intended to protect.

[0104] Unless otherwise specified, the experimental methods used in the following examples are all conventional methods; the reagents, materials, etc. used in the following examples, unless otherwise specified, can be obtained from commercial channels.

[0105] The present invention uses a combination of indoor bioassay and field test to test the insecticidal effect of the composition. It should be noted that any modification, equivalent substitution and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

[0106] 1. Preparation Example:

[0107] Preparation Example 1: Wettable Powder

[0108]

[0109] The above materials are roughly crushed according to the mass ratio, put into the mixer and mixed evenly, and then the finished product is obtained after air flow crushing.

[0110] Preparation Example 2: Water Dispersible Granules

[0111]

[0112]

[0113] The effective active ingredients and various auxiliary agents are mixed evenly according to the mass ratio of the formula, and the powder is obtained after air flow crushing. A certain amount of water is added to mix and extrude granulate, and the finished product is obtained after drying and screening.

[0114] Preparation Example 3: Suspension

[0115]

[0116] The effective active ingredients and various auxiliary agents are mixed evenly according to the mass ratio of the formula, and the finished product is obtained after high-speed shearing and sand grinding.

[0117] Preparation Example 4: Emulsifiable Concentrate

[0118]

[0119] The above raw materials are mixed according to the mass ratio and dissolved into a uniform oil phase; after passing the inspection, they are measured and packaged to obtain the finished product.

[0120] Preparation Example 5: Emulsion in water

[0121]

[0122]

[0123] According to the formula requirements, the above raw materials are added into the batching kettle according to the mass ratio, and evenly mixed by a high-speed shearing machine to form a water emulsion. After passing the inspection, it is measured and packaged to obtain the finished product.

[0124] Preparation Example 6: Emulsifiable Concentrate

[0125]

[0126] The above raw materials are mixed according to the mass ratio and dissolved into a uniform oil phase; after passing the inspection, they are measured and packaged to obtain the finished product.

[0127] Preparation Example 7: Granules

[0128]

[0129] The components are fully mixed according to the mass ratio of the formula, crushed, moistened with water and then fully stirred, and then granulated with a screw extrusion granulator, dried and sieved to obtain the finished product.

[0130] 2. Indoor Toxicity Determination Example

[0131] (1) Test pests: two-spotted spider mite (Tetranychus urticae Koch), cinnabarinus spider mite (Tetranychus cinnabarinus), diamondback moth (Plutella xylostella (Linnaeus)).

[0132] (2) Determination method:

[0133] Pest toxicity assay

[0134] Pest toxicity determination The pests such as two-spotted spider mite, cinnabarinus spider mite and diamondback moth were used as test materials. The leaf dipping method (for diamondback moth) and spider mite slide dipping method (for mites) were used to determine the toxicity of compound I, compound II and their mixed preparations.

[0135] Referring to the Agricultural Industry Standard of the People's Republic of China NY / T 1154.12-2008 "Guidelines for Indoor Bioassay Tests of Pesticides" Insecticide Part 12: Slide Dipping Method for Spider Mites, the test agents (including Compound I and Compound II) were first prepared into 5 concentration gradients (based on the LC of the compounds on different pests) with a suitable solvent (the type of solvent is acetone, methanol, N, N dimethylformamide and dimethyl sulfoxide, etc., and is selected based on its solubility for the sample). 50 value, setting the concentration gradient according to the geometric ratio or arithmetic difference), compound I and compound II for mixing are respectively 50 The values ​​are set to a series of ratios according to mass ratios, and the final mass concentration (referring to the total mass of compound I and compound II) is prepared according to different ratios.

[0136] Cut the double-sided tape into 2 cm long pieces and stick them to one end of the slide. Then select healthy female adult mites and stick their backs to the double-sided tape (be careful not to stick to the mite legs, antennae and mouthparts). Place 30 mites per slide in a container lined with a wet sponge, cover with a lid, and place at (25±1)℃. After 2 hours, examine under a microscope, remove dead and injured individuals, and make up 30 mites per slide. Dip the slide in the drug solution and shake gently for 5 seconds, then take it out, absorb the excess drug solution with absorbent paper, place it in a white porcelain plate lined with a wet sponge, and cover it with a plastic film with good light transmittance. Repeat 4 times for each treatment, and set a treatment without drug (containing organic solvents and emulsifiers) as a blank control.

[0137] Referring to the Pesticide Part 14: Leaf Dipping Method of the Agricultural Industry Standard of the People's Republic of China NY / T 1154.14-2008 "Guidelines for Indoor Bioassay Tests of Pesticides", the test agents (including Compound I and Compound II) were first prepared into 5 concentration gradients (based on the LC of the compounds on different pests) with a suitable solvent (the type of solvent is acetone, methanol, N, N-dimethylformamide and dimethyl sulfoxide, etc., and is selected based on its solubility for the sample). 50 value, setting the concentration gradient according to the geometric ratio or arithmetic difference), compound I and compound II for mixing are respectively 50 The values ​​are set to a series of ratios according to mass ratios, and the final mass concentration (referring to the total mass of compound I and compound II) is prepared according to different ratios.

[0138] Immerse the leaf disc or leaf segment in the solution of the test agent for 10 seconds (the immersion time can be appropriately extended or shortened according to the characteristics of the agent), take it out and dry it, place it in a petri dish containing 1% water agar or moisturizing filter paper, and inoculate the test insects, with no less than 10 heads per repetition. Each treatment should be repeated at least 4 times, and a treatment without the agent (including all organic solvents and emulsifiers) should be set as a blank control.

[0139] At the same time, the co-toxicity coefficient (CTC value) of the two drugs in different ratios is calculated according to Sun Yunpei's method. The co-toxicity coefficient (CTC) of the compound preparation ≥ 120 shows a synergistic effect, CTC ≤ 80 shows an antagonistic effect; 80 < CTC < 120 shows an additive effect. The co-toxicity coefficient (CTC) of the mixture is calculated according to formula (1), formula (2), and formula (3):

[0140]

[0141] Where:

[0142] ATI——Actual Toxicity Index of Mixture;

[0143] S——LC of a single dose 50 , the unit is milligrams per liter (mg / L);

[0144] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L);

[0145] TTI=TI A ×P A +TI B ×P B (2)

[0146] Where:

[0147] TTI——Theoretical Toxicity Index of Mixtures;

[0148] TI A ——A: toxicity index of the agent;

[0149] P A ——The percentage of agent A in the mixture, in percentage (%);

[0150] TI B ——Toxicity index of agent B;

[0151] P B ——The percentage of agent B in the mixture, in percentage (%).

[0152]

[0153] Where:

[0154] CTC – Co-toxicity coefficient;

[0155] ATI——Actual Toxicity Index of Mixture;

[0156] TTI - Theoretical Toxicity Index of Mixtures.

[0157] When different types of pesticides are mixed, they usually show three types of effects, namely additive effect, synergistic effect and antagonistic effect, but it is impossible to predict what kind of effect it is. The synergistic composition of the present invention uses compound I and trifluoxetine or LHP-560 as effective active ingredients, and is illustrated by a bioassay example.

[0158] Experiment on the activity determination of pesticides for indoor use of spider mite

[0159] In order to verify the effect of different ratios of compound I and trifluoxetine on spider mites, indoor toxicity determination of different ratios of compound I and trifluoxetine on spider mites was carried out. The corresponding concentrations were prepared according to the weight percentage in the table, and indoor mite killing tests were carried out. The test results are as follows:

[0160] Table 1 The results of toxicity test of compound I and triflumuron monotherapy and their mixtures against spider mite

[0161] Active ingredients Ratio Regression equation <![CDATA[LC 50 ]]> Co-toxicity coefficient evaluate Compound I — y=3.7283+1.6399x 5.9633 — — Trifloxystrobin — y=3.8238+2.1495x 3.5252 — — Compound I: Trifluoxetine 50：1 y=3.8833+1.9335x 3.7803 155.6370 Efficiency Compound I: Trifluoxetine 10：1 y=4.3351+1.6453x 2.5357 221.2633 Efficiency Compound I: Trifluoxetine 1：1 y=4.5970+1.6640x 1.7466 253.6935 Efficiency Compound I: Trifluoxetine 1：10 y=4.7237+1.8294x 1.4160 258.5642 Efficiency Compound I: Trifluoxetine 1：50 y=4.4083+1.6435x 2.2910 155.1147 Efficiency

[0162] From the results in Table 1, it can be seen that the mixture of compound I and triflumuron in different ratios and dosages has a significant synergistic effect on the activity of two-spotted spider mite.

[0163] In order to verify the effect of different ratios of compound I and LHP-560 on spider mites, indoor toxicity test of different ratios of compound I and LHP-560 on spider mites was carried out. The corresponding concentrations were prepared according to the weight percentage in the table, and indoor mite killing test was carried out. The test results are as follows:

[0164] Table 2 Toxicity test results of compound I and LHP-560 single agent and their mixture to Tetranychus urticae

[0165] Active ingredients Ratio Regression equation <![CDATA[LC 50 ]]> Co-toxicity coefficient evaluate Compound I — y=3.7283+1.6399x 5.9633 — — LHP-560 — y=3.9688+1.9633x 3.3514 — — Compound I: LHP-560 50：1 y=3.9750+1.8618x 3.5528 165.3226 Efficiency Compound I: LHP-560 10：1 y=4.3631+1.5044x 2.6506 210.0955 Efficiency Compound I: LHP-560 1：1 y=4.627+1.6188x 1.6998 252.4505 Efficiency Compound I: LHP-560 1：10 y=4.7745+1.7553x 1.3443 259.6418 Efficiency Compound I: LHP-560 1：50 y=4.4537+1.5561x 2.2443 150.6225 Efficiency

[0166] From the results in Table 2, it can be seen that the mixture of compound I and LHP-560 in different ratios and dosages has a significant synergistic effect on the activity of two-spotted spider mites.

[0167] Experiment on the activity determination of indoor pesticides against Tetranychus cinnabarinus

[0168] In order to verify the effect of different ratios of compound I and trifluoxetine on spider mites, indoor toxicity tests of different ratios of compound I and trifluoxetine on spider mites were carried out. The corresponding concentrations were prepared according to the weight percentages in the table, and indoor mite killing tests were carried out. The test results are as follows:

[0169] Table 3 Toxicity test results of compound I and trifluprostol monotherapy and their mixtures against Tetranychus cinnabarinus

[0170] Active ingredients Ratio Regression equation <![CDATA[LC 50 ]]> Co-toxicity coefficient evaluate Compound I — y=4.4420+1.5276x 2.3190 — — Trifloxystrobin — y=3.9679+1.8917x 3.5121 — — Compound I: Trifluoxetine 50：1 y=4.7467+1.7692x 1.3905 167.8924 Efficiency Compound I: Trifluoxetine 10：1 y=4.6772+1.6104x 1.5866 150.8189 Efficiency Compound I: Trifluoxetine 1：1 y=4.7298+1.4181x 1.5508 180.1322 Efficiency Compound I: Trifluoxetine 1：10 y=4.5503+1.4565x 2.0359 164.8012 Efficiency Compound I: Trifluoxetine 1：50 y=4.5079+1.5245x 2.1027 165.3606 Efficiency

[0171] From the results in Table 3, it can be seen that the mixture of compound I and trifluoxetine in different ratios and dosages has a significant synergistic effect on the activity of Tetranychus cinnabarinus.

[0172] In order to verify the effect of different ratios of compound I and LHP-560 on spider mites, indoor toxicity tests on spider mites were carried out using different ratios of compound I and LHP-560. The corresponding concentrations were prepared according to the weight percentages in the table, and indoor mite killing tests were carried out. The test results are as follows:

[0173] Table 4 Toxicity test results of compound I and LHP-560 single agent and their mixture to Tetranychus cinnabarinus

[0174]

[0175]

[0176] From the results in Table 4, it can be seen that the mixture of compound I and LHP-560 in different ratios and dosages has a significant synergistic effect on the activity of Tetranychus cinnabarinus.

[0177] Experiment on the activity determination of indoor pesticides for Plutella xylostella

[0178] In order to verify the effects of different ratios of compound I and trifluoxetine on Plutella xylostella, indoor toxicity tests of different ratios of compound I and trifluoxetine on Plutella xylostella were carried out. The corresponding concentrations were prepared according to the weight percentages in the table, and indoor insecticidal tests were carried out. The test results are as follows:

[0179] Table 5 Toxicity test results of compound I and trifluproline single agent and its mixture to Plutella xylostella

[0180] Active ingredients Ratio Regression equation <![CDATA[LC 50 ]]> Co-toxicity coefficient evaluate Compound I — y=2.2593+1.6230x 48.8240 — — Trifloxystrobin — y=2.7637+1.8504x 16.1633 — — Compound I: Trifluoxetine 50：1 y=2.5718+1.6707x 28.4053 165.3353 Efficiency Compound I: Trifluoxetine 10：1 y=2.7405+1.6590x 23.0121 179.2434 Efficiency Compound I: Trifluoxetine 1：1 y=3.0926+1.6285x 14.8332 163.7307 Efficiency Compound I: Trifluoxetine 1：10 y=2.8231+2.0961x 10.9276 157.4891 Efficiency Compound I: Trifluoxetine 1：50 y=2.7889+2.0585x 11.8613 138.0796 Efficiency

[0181] From the results in Table 5, it can be seen that the mixture of compound I and triflumuron in different ratios and dosages has a significant synergistic effect on the activity of Plutella xylostella.

[0182] In order to verify the effects of different ratios of compound I and LHP-560 on diamondback moth, indoor toxicity tests of different ratios of compound I and LHP-560 on diamondback moth were carried out. The corresponding concentrations were prepared according to the weight percentages in the table, and indoor insecticidal tests were carried out. The test results are as follows:

[0183] Table 6 Toxicity test results of compound I and LHP-560 single agent and mixture to Plutella xylostella

[0184] Active ingredients Ratio Regression equation <![CDATA[LC 50 ]]> Co-toxicity coefficient evaluate Compound I — y=2.2593+1.6230x 48.8240 — — LHP-560 — y=6.5219+1.7081x 0.1285 — — Compound I: LHP-560 50：1 y=3.6777+2.1126x 4.2258 137.0965 Efficiency Compound I: LHP-560 10：1 y=5.2068+1.6923x 0.7548 182.4836 Efficiency Compound I: LHP-560 1：1 y=6.2622+1.2756x 0.1024 250.3178 Efficiency Compound I: LHP-560 1：10 y=6.4715+1.3425x 0.0801 176.4187 Efficiency Compound I: LHP-560 1：50 y=6.2693+1.2904x 0.1038 126.2640 Efficiency

[0185] From the results in Table 6, it can be seen that the mixture of compound I and LHP-560 in different ratios and dosages has a significant synergistic effect on the activity of Plutella xylostella.

[0186] 3. Field efficacy embodiment

[0187] In order to clarify the control effects of compound I, triflumuron and LHP-560 on two-spotted spider mites, citrus mites and diamondback moth, several efficacy tests were carried out in different regions of China. The efficacy calculation method is as follows:

[0188]

[0189]

[0190] Note: In the following table, it is referred to as “prevention effect”.

[0191] Field test on the efficacy of pesticides against Panonychus citrus

[0192] Test method: According to the application method specified in the National Standard of the People's Republic of China GB / T 17980.11-2000, the pesticide is usually applied when there are an average of 2 live mites per leaf.

[0193] Survey method: According to the survey method specified in the National Standard of the People's Republic of China GB / T 17980.11-2000, mark the tender shoots in the east, west, south, north and center of the tree in each plot, and survey the number of active mites on a total of 25 leaves. Use a handheld magnifying glass to directly observe the leaf surface and count the number of mites. If the damage is light, the number of leaves surveyed can be increased. Conduct a base survey on the eve of treatment, and conduct a survey once 1 day, 3 days, 10 days, 15 days, 20 days, and 30 days after treatment, and record the number of active mites.

[0194] Field test on the efficacy of pesticides against two-spotted spider mite

[0195] According to the application method specified in the "Guidelines for Field Efficacy Tests of Pesticides (GB / T 17980.17-2000)", peppers were sprayed on the stems and leaves.

[0196] Survey method: According to the survey method specified in the Guidelines for Field Efficacy Tests of Pesticides (GB / T 17980.17-2000), 20 leaves were taken from each plot to count the number of live and nymph mites. Surveys were conducted 1, 3, 7, and 14 days after the application of pesticides.

[0197] Field test on the efficacy of pesticides against Plutella xylostella

[0198] Test method: The pesticide application method is based on the National Standard of the People's Republic of China GB / T 17980.13-2000. The first application is carried out when there are sufficient larvae (1 to 3 pre-third instar larvae on each plant).

[0199] Survey method: According to the survey method specified in the National Standard of the People's Republic of China GB / T 17980.13-2000, count the number of live larvae of different ages on no less than 10 crops in each plot, and survey the entire plant. Investigate the insect population base before application, and investigate the number of live insects 1-3 days and 7-14 days after treatment. In the following table, "10% Compound I·Trifluanid WP (1:1)" means that the total mass fraction of the active ingredient is 10%, and 1:1 refers to the mass ratio of Compound I to Trifluanid, and so on.

[0200] Table 7 Field efficacy test of compound I and triflumuron against citrus mites

[0201]

[0202]

[0203] Note: Different letters after the data in the same column indicate significant differences at the P<0.05 level as tested by Duncan's new multiple range test, the same below.

[0204] It can be seen from the test results in Table 7 that when controlling citrus mites, the compound I and trifluoxetine combined with the single agent have significantly higher 1-day, 3-day, 10-day, 15-day, 20-day and 30-day control effects on citrus mites than the single agent at the same dosage of the active ingredient. The 30-day control effect can still reach more than 90.80%, and the duration of effect is relatively long.

[0205] Table 8 Field efficacy test of compound I and LHP-560 against Panonychus citri

[0206]

[0207]

[0208] The test results in Table 8 show that, when controlling Panonychus citri, the compound I and LHP-560 combined with the single agent have significantly higher 1-day, 3-day, 10-day, 15-day, 20-day and 30-day control effects on Panonychus citri than the single agent at the same dosage of active ingredients. The 30-day control effect can still reach more than 90.66%, with a longer duration.

[0209] Table 9 Field efficacy test of compound I combined with triflumuron against Tetranychus urticae

[0210]

[0211] The test results in Table 9 show that, when controlling spider mites, the compound I and trifluoxetine are compared with the single agent. At the same dosage of active ingredients, the compound I and trifluoxetine have significantly higher 1-day, 3-day, 7-day and 14-day control effects on spider mites than the single agent. The 14-day control effect can still reach more than 91.93%, and the duration of effect is relatively long.

[0212] Table 10 Field efficacy test of compound I combined with LHP-560 against Tetranychus urticae

[0213]

[0214]

[0215] The test results in Table 10 show that, when controlling spider mites, the compound I and LHP-560 combined with the single agent have significantly higher 1-day, 3-day, 7-day and 14-day control effects on spider mites than the single agent at the same dosage of active ingredients. The 14-day control effect can still reach more than 87.61%, and the duration of effect is relatively long.

[0216] Table 11 Field efficacy test of compound I and triflupromazine against Plutella xylostella

[0217]

[0218] The test results in Table 11 show that, when controlling diamondback moth, the compound I and trifluoxetine are compared with the single agent, and at the same dosage of the active ingredient, the compound I and trifluoxetine have significantly higher 1-day, 3-day and 7-day control effects on diamondback moth than the single agent. The 7-day control effect can still reach more than 77.66%.

[0219] Table 12 Field efficacy test of compound I and LHP-560 against Plutella xylostella

[0220]

[0221] The test results in Table 12 show that, when controlling diamondback moth, the compound I and LHP-560 combined with the single agent have significantly higher 1-day, 3-day and 7-day control effects on diamondback moth than the single agent at the same dosage of active ingredient. The 7-day control effect can still reach more than 92.73%, with a longer duration.

[0222] The above is an explanation of the embodiments of the present invention. However, the present invention is not limited to the above embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A synergistic composition containing Sulfiflumin, wherein the synergistic composition comprises an active ingredient, wherein the active ingredient comprises compound I and compound II, wherein: The compound I is selected from Sulfiflumin, and the compound II is selected from one of LHP-560 and trifluoxetine or a mixture thereof; Wherein, the structural formula of compound I is shown below: The structural formula of LHP-560 is shown below:

2. The synergistic composition according to claim 1, characterized in that The mass ratio of the compound I to the compound II is 80-1:1-80, for example, 50-1:1-50.

3. The synergistic composition according to claim 1 or 2, characterized in that: Based on the total weight of the synergistic composition being 100%, the sum of the weights of the compound I and the compound II in the composition is 1 to 80%, and the rest are auxiliary materials; Preferably, the synergistic composition comprises Sulfiflumin and LHP-560; Preferably, the synergistic composition comprises Sulfiflumin and trifloxystrobin; Preferably, the synergistic combination comprises Sulfiflumin, LHP-560 and trifloxystrobin.

4. The synergistic composition according to any one of claims 1 to 3, characterized in that The synergistic composition is prepared into a liquid preparation or a solid preparation; Preferably, when it is a liquid preparation, the liquid preparation contains 1% to 60% of the effective active ingredient by weight, and the rest are excipients; when it is a solid preparation, the solid preparation contains 5% to 80% of the effective active ingredient by weight, and the rest are excipients; Preferably, the auxiliary materials include at least one of the following: deionized water, organic solvents, emulsifiers, dispersants, wetting agents, thickeners, defoamers, stabilizers, binders, disintegrants, antifreeze agents, anticaking agents, suspending agents, film formers, preservatives, colorants, polymer capsule wall materials, pH regulators or fillers.

5. Use of the synergistic composition according to any one of claims 1 to 4 for controlling pests and mites in agriculture and non-agriculture; Preferably, the pest is of the order Lepidoptera, Hemiptera, Coleoptera, Thysanoptera or Diptera; Preferably, the pest is selected from Plutella xylostella. Preferably, the mite is selected from Panonychus citri, Tetranychus urticae or Tetranychus cinnabarinus.

6. A method for controlling agricultural or non-agricultural pests and mites, comprising applying the synergistic composition according to any one of claims 1 to 4 to plants where pests and mites exist.

7. A composition for controlling invertebrate pests, comprising a biologically effective amount of the synergistic composition according to any one of claims 1 to 4 and at least one other component selected from a surfactant, a solid diluent and a liquid diluent, the composition optionally containing an effective amount of at least one other biologically active compound or active agent; Preferably, the invertebrate pest is selected from Plutella xylostella.

8. A method for controlling invertebrate pests, comprising contacting the invertebrate pests or their environment with a biologically effective amount of the invertebrate pest controlling composition of claim 7. Preferably, the invertebrate pest is selected from Plutella xylostella.

9. A spray composition comprising the synergistic composition according to any one of claims 1 to 4 and a propellant.

10. A bait composition comprising the synergistic composition of any one of claims 1 to 4; one or more food materials; optionally an attractant; and optionally a wetting agent.

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